CN1198924C - 在丝状真菌中表达克隆 - Google Patents

在丝状真菌中表达克隆 Download PDF

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Publication number
CN1198924C
CN1198924C CN98812563.3A CN98812563A CN1198924C CN 1198924 C CN1198924 C CN 1198924C CN 98812563 A CN98812563 A CN 98812563A CN 1198924 C CN1198924 C CN 1198924C
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cdna
dna
expression
gene
vector
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Expired - Lifetime
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CN98812563.3A
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English (en)
Chinese (zh)
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CN1283227A (zh
Inventor
J·M·范丹布林克
G·C·M·塞尔坦
J·P·T·W·范丹弘波夫
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Koninklijke DSM NV
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DSM NV
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1086Preparation or screening of expression libraries, e.g. reporter assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Mushroom Cultivation (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Peptides Or Proteins (AREA)
CN98812563.3A 1997-12-22 1998-12-22 在丝状真菌中表达克隆 Expired - Lifetime CN1198924C (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP97204079.4 1997-12-22
EP97204079 1997-12-22

Publications (2)

Publication Number Publication Date
CN1283227A CN1283227A (zh) 2001-02-07
CN1198924C true CN1198924C (zh) 2005-04-27

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ID=8229117

Family Applications (1)

Application Number Title Priority Date Filing Date
CN98812563.3A Expired - Lifetime CN1198924C (zh) 1997-12-22 1998-12-22 在丝状真菌中表达克隆

Country Status (13)

Country Link
US (1) US20020155536A1 (https=)
EP (2) EP1854890A1 (https=)
JP (3) JP4410413B2 (https=)
CN (1) CN1198924C (https=)
AT (1) ATE367439T1 (https=)
AU (1) AU736111C (https=)
BR (1) BR9814404A (https=)
CA (1) CA2313445C (https=)
DE (1) DE69838106T2 (https=)
DK (1) DK1042461T3 (https=)
ES (1) ES2287989T3 (https=)
PL (1) PL341760A1 (https=)
WO (1) WO1999032617A2 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102286483B (zh) * 2004-04-16 2014-06-04 中化帝斯曼制药有限公司荷兰公司 用于在真菌细胞中表达基因的真菌启动子

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AU5354401A (en) * 2000-04-13 2001-10-30 Emalfarb, Mark Aaron High-throughput screening of expressed DNA libraries in filamentous fungi
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WO2004078919A2 (en) * 2003-02-27 2004-09-16 Midwest Research Institute Superactive cellulase formulation using cellobiohydrolase-1 from penicillium funiculosum
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US9045748B2 (en) 2004-05-27 2015-06-02 Novozymes, Inc. Methods for transforming and expression screening of filamentous fungal cells with a DNA library
EA014853B1 (ru) * 2004-10-15 2011-02-28 ДСМ АйПи АССЕТС Б.В. Амидаза из aspergillus niger и применение амидазы для получения пищевого продукта с пониженным содержанием акриламида
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US8551751B2 (en) 2007-09-07 2013-10-08 Dyadic International, Inc. BX11 enzymes having xylosidase activity
CN102741421B (zh) 2009-03-10 2015-03-25 帝斯曼知识产权资产管理有限公司 用于提高多肽产率的方法
DK2406372T3 (da) 2009-03-10 2017-11-27 Dsm Ip Assets Bv Prægastrisk esterase og derivater deraf
EP2421986A1 (en) 2009-04-22 2012-02-29 DSM IP Assets B.V. Process for the production of a recombinant polypeptide of interest
EP3293264A1 (en) 2009-07-22 2018-03-14 DSM IP Assets B.V. Improved host cell for the production of a compound of interest
IN2012DN03186A (https=) 2009-11-04 2015-09-25 Dsm Ip Assets Bv
EP2531590A2 (en) 2010-02-04 2012-12-12 DSM IP Assets B.V. Process for preparing filamentous fungal strains having a sexual cycle and a process for preparing sexually crossed filamentous fungal strains
EA201201125A1 (ru) 2010-02-11 2013-03-29 ДСМ АйПи АССЕТС Б.В. Клетка-хозяин, способная продуцировать ферменты, пригодные для деградации лигноцеллюлозного материала
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US20150197760A1 (en) 2012-06-19 2015-07-16 Dsm Ip Assets B.V. Promoters for expressing a gene in a cell
JP6468561B2 (ja) 2012-07-19 2019-02-13 ディーエスエム アイピー アセッツ ビー.ブイ.Dsm Ip Assets B.V. AgsE欠損株
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WO2014142647A1 (en) 2013-03-14 2014-09-18 Wageningen Universiteit Fungals strains with improved citric acid and itaconic acid production
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WO2014202620A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202622A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
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CN105940103B (zh) * 2014-01-31 2021-06-15 株式会社Api 哌可酸4位羟化酶和利用该酶的4-羟基氨基酸的制造法
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US10450554B2 (en) 2014-05-19 2019-10-22 Dsm Ip Assets B.V. Proline specific endoprotease
WO2015177152A1 (en) 2014-05-19 2015-11-26 Dsm Ip Assets B.V. Proline-specific endoprotease
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AR104205A1 (es) 2015-04-09 2017-07-05 Dsm Ip Assets Bv Fosfolipasa c
WO2016193292A1 (en) 2015-06-02 2016-12-08 Dsm Ip Assets B.V. Use of ice structuring protein afp19 expressed in filamentous fungal strains for preparing food
EP4361240A3 (en) 2015-11-17 2024-07-31 DSM IP Assets B.V. Preparation of a stable beer
WO2018019948A1 (en) 2016-07-29 2018-02-01 Dsm Ip Assets B.V. Polypeptides having cellulolytic enhancing activity and uses thereof
ES2784318T3 (es) * 2016-09-20 2020-09-24 Clariant Int Ltd Método para una expresión selectiva independiente de la fuente de carbono de secuencias que codifican una proteína en una célula de hongo filamentoso
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102286483B (zh) * 2004-04-16 2014-06-04 中化帝斯曼制药有限公司荷兰公司 用于在真菌细胞中表达基因的真菌启动子

Also Published As

Publication number Publication date
JP2002509698A (ja) 2002-04-02
EP1042461B1 (en) 2007-07-18
CA2313445A1 (en) 1999-07-01
WO1999032617A2 (en) 1999-07-01
JP2005261438A (ja) 2005-09-29
US20020155536A1 (en) 2002-10-24
EP1042461A2 (en) 2000-10-11
AU2515199A (en) 1999-07-12
ATE367439T1 (de) 2007-08-15
DK1042461T3 (da) 2007-09-24
JP4410413B2 (ja) 2010-02-03
EP1854890A1 (en) 2007-11-14
CA2313445C (en) 2012-03-20
ES2287989T3 (es) 2007-12-16
BR9814404A (pt) 2000-10-10
JP2010046080A (ja) 2010-03-04
DE69838106D1 (de) 2007-08-30
CN1283227A (zh) 2001-02-07
WO1999032617A3 (en) 1999-09-10
AU736111B2 (en) 2001-07-26
AU736111C (en) 2004-02-19
PL341760A1 (en) 2001-05-07
DE69838106T2 (de) 2008-04-03

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