ATE367439T1 - Expressionsklonierung in filamentösen pilzen - Google Patents
Expressionsklonierung in filamentösen pilzenInfo
- Publication number
- ATE367439T1 ATE367439T1 AT98966868T AT98966868T ATE367439T1 AT E367439 T1 ATE367439 T1 AT E367439T1 AT 98966868 T AT98966868 T AT 98966868T AT 98966868 T AT98966868 T AT 98966868T AT E367439 T1 ATE367439 T1 AT E367439T1
- Authority
- AT
- Austria
- Prior art keywords
- expression cloning
- filamentous fungi
- interest
- dna sequences
- proteins
- Prior art date
Links
- 238000001400 expression cloning Methods 0.000 title abstract 2
- 241000233866 Fungi Species 0.000 title 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 230000002538 fungal effect Effects 0.000 abstract 1
- 238000002955 isolation Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP97204079 | 1997-12-22 |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE367439T1 true ATE367439T1 (de) | 2007-08-15 |
Family
ID=8229117
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT98966868T ATE367439T1 (de) | 1997-12-22 | 1998-12-22 | Expressionsklonierung in filamentösen pilzen |
Country Status (13)
Country | Link |
---|---|
US (1) | US20020155536A1 (de) |
EP (2) | EP1854890A1 (de) |
JP (3) | JP4410413B2 (de) |
CN (1) | CN1198924C (de) |
AT (1) | ATE367439T1 (de) |
AU (1) | AU736111C (de) |
BR (1) | BR9814404A (de) |
CA (1) | CA2313445C (de) |
DE (1) | DE69838106T2 (de) |
DK (1) | DK1042461T3 (de) |
ES (1) | ES2287989T3 (de) |
PL (1) | PL341760A1 (de) |
WO (1) | WO1999032617A2 (de) |
Families Citing this family (64)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7883872B2 (en) | 1996-10-10 | 2011-02-08 | Dyadic International (Usa), Inc. | Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose |
ATE376057T1 (de) | 1999-10-01 | 2007-11-15 | Novozymes As | Enzymgranulat |
WO2001079558A1 (en) * | 2000-04-13 | 2001-10-25 | Mark Aaron Emalfarb | High-throughput screening of expressed dna libraries in filamentous fungi |
US7122330B2 (en) | 2000-04-13 | 2006-10-17 | Mark Aaron Emalfarb | High-throughput screening of expressed DNA libraries in filamentous fungi |
BR0017339A (pt) | 2000-09-21 | 2004-07-27 | Dsm Nv | Xilanases de talaromices |
JP4119248B2 (ja) | 2000-12-07 | 2008-07-16 | ディーエスエム アイピー アセッツ ビー.ブイ. | カルボキシ末端プロリンを有するペプチドに富むタンパク質加水分解物 |
AU2002358179A1 (en) | 2001-12-21 | 2003-07-09 | Dsm Ip Assets B.V. | New rennets |
TWI328612B (en) | 2002-07-25 | 2010-08-11 | Dsm Ip Assets Bv | Genes and their encoded polypeptides involved in the biosynthetic pathway of vitamin b12, vectors and host cells comprising the genes, and process for producing vitamin b12 |
WO2004078919A2 (en) * | 2003-02-27 | 2004-09-16 | Midwest Research Institute | Superactive cellulase formulation using cellobiohydrolase-1 from penicillium funiculosum |
US20050059024A1 (en) | 2003-07-25 | 2005-03-17 | Ambion, Inc. | Methods and compositions for isolating small RNA molecules |
CN102286483B (zh) * | 2004-04-16 | 2014-06-04 | 中化帝斯曼制药有限公司荷兰公司 | 用于在真菌细胞中表达基因的真菌启动子 |
CA2565888C (en) * | 2004-05-27 | 2014-10-14 | Novozymes, Inc. | Methods for transforming and expression screening of filamentous fungal cells with a dna library |
BRPI0516523A (pt) * | 2004-10-15 | 2008-09-09 | Dsm Ip Assets Bv | processo para a produção de alimento |
JP2008527985A (ja) | 2005-01-24 | 2008-07-31 | ディーエスエム アイピー アセッツ ビー.ブイ. | 糸状菌細胞において目的の化合物を産生させるための方法 |
US20090209007A1 (en) * | 2005-03-18 | 2009-08-20 | Roelf Bernhard Meima | Method of Expression Cloning in a Host Cell |
EA014783B1 (ru) | 2005-11-29 | 2011-02-28 | ДСМ АйПи АССЕТС Б.В. | Связывающий днк-сайт активатора транскрипции, пригодный для экспрессии генов |
CN101400694B (zh) | 2005-12-01 | 2013-07-24 | 阿德库端木营养控股有限责任公司 | 用于工业生产柠檬酸的基因 |
ES2534282T3 (es) | 2006-06-29 | 2015-04-21 | Dsm Ip Assets B.V. | Un método para lograr la expresión polipeptídica mejorada |
EP2511372A1 (de) | 2006-11-02 | 2012-10-17 | DSM IP Assets B.V. | Verbesserte Herstellung von sekretierten Proteinen durch filamentöse Pilze |
WO2008073914A2 (en) | 2006-12-10 | 2008-06-19 | Dyadic International Inc. | Expression and high-throughput screening of complex expressed dna libraries in filamentous fungi |
EP2631295A3 (de) | 2007-02-15 | 2014-01-01 | DSM IP Assets B.V. | Rekombinante Hostzelle zur Herstellung einer Verbindung von Interesse |
CA2676686C (en) | 2007-02-20 | 2015-12-15 | Dsm Ip Assets B.V. | Novel sialidase |
CA2736661A1 (en) | 2007-09-07 | 2009-03-12 | Dyadic International, Inc. | Novel fungal enzymes |
BRPI1009189A2 (pt) | 2009-03-10 | 2015-09-15 | Dsm Ip Assets Bv | método para melhorar o rendimento de um polipetídeo |
EP2406372B1 (de) | 2009-03-10 | 2017-08-23 | DSM IP Assets B.V. | Prägastrische esterase und derivate davon |
US20120041171A1 (en) | 2009-04-22 | 2012-02-16 | Dsm Ip Assets B.V. | Process for the production of a recombinant polypeptide of interest |
EP2456872B1 (de) | 2009-07-22 | 2017-08-30 | DSM IP Assets B.V. | Verbesserte wirtszelle für die herstellung von relevanten produkten |
WO2011054899A1 (en) | 2009-11-04 | 2011-05-12 | Dsm Ip Assets B.V. | Talaromyces transformants |
US20130011876A1 (en) | 2010-02-04 | 2013-01-10 | Dsm Ip Assetts, B.V. | Process for preparing filamentous fungal strains having a sexual cycle and a process for preparing sexually crossed filamentous fungal strains |
WO2011098577A1 (en) | 2010-02-11 | 2011-08-18 | Dsm Ip Assets B.V. | Host cell capable of producing enzymes useful for degradation of lignocellulosic material |
EP2588616B1 (de) | 2010-07-01 | 2018-11-14 | DSM IP Assets B.V. | Verfahren zur herstellung einer bestimmten verbindung |
WO2012007446A1 (en) | 2010-07-13 | 2012-01-19 | Dsm Ip Assets B.V. | Use of a mutarotase in the production of dried powders |
WO2012007445A1 (en) | 2010-07-13 | 2012-01-19 | Dsm Ip Assets B.V. | Mutarotase in crystallization |
US20150197760A1 (en) | 2012-06-19 | 2015-07-16 | Dsm Ip Assets B.V. | Promoters for expressing a gene in a cell |
US10035986B2 (en) | 2012-07-19 | 2018-07-31 | Dsm Ip Assets B.V. | AgsE-deficient strain |
ES2923460T3 (es) | 2013-02-04 | 2022-09-27 | Dsm Ip Assets Bv | Polipéptido degradador de carbohidratos y sus usos |
WO2014142647A1 (en) | 2013-03-14 | 2014-09-18 | Wageningen Universiteit | Fungals strains with improved citric acid and itaconic acid production |
WO2014202624A2 (en) | 2013-06-19 | 2014-12-24 | Dsm Ip Assets B.V. | Rasamsonia gene and use thereof |
WO2014202620A2 (en) | 2013-06-19 | 2014-12-24 | Dsm Ip Assets B.V. | Rasamsonia gene and use thereof |
WO2014202622A2 (en) | 2013-06-19 | 2014-12-24 | Dsm Ip Assets B.V. | Rasamsonia gene and use thereof |
WO2014202621A1 (en) | 2013-06-20 | 2014-12-24 | Dsm Ip Assets B.V. | Carbohydrate degrading polypeptide and uses thereof |
WO2015082488A1 (en) | 2013-12-02 | 2015-06-11 | Dsm Ip Assets B.V. | Ice structuring protein |
JP6729963B2 (ja) * | 2014-01-31 | 2020-07-29 | 株式会社エーピーアイ コーポレーション | ピペコリン酸4位水酸化酵素およびそれを利用した4−ヒドロキシアミノ酸の製造法 |
WO2015177152A1 (en) | 2014-05-19 | 2015-11-26 | Dsm Ip Assets B.V. | Proline-specific endoprotease |
US10450554B2 (en) | 2014-05-19 | 2019-10-22 | Dsm Ip Assets B.V. | Proline specific endoprotease |
WO2015177153A1 (en) | 2014-05-19 | 2015-11-26 | Dsm Ip Assets B.V. | Proline-specific endoprotease |
DK3242950T3 (da) | 2015-01-06 | 2021-12-20 | Dsm Ip Assets Bv | Crispr-cas-system til en trådformet svampeværtscelle |
AR104205A1 (es) | 2015-04-09 | 2017-07-05 | Dsm Ip Assets Bv | Fosfolipasa c |
MX2017015126A (es) | 2015-06-02 | 2018-03-28 | Dsm Ip Assets Bv | Uso de proteina estructurante del hielo. |
US10913938B2 (en) | 2016-07-29 | 2021-02-09 | Dsm Ip Assets B.V. | Polypeptides having cellulolytic enhancing activity and uses thereof |
ES2784318T3 (es) * | 2016-09-20 | 2020-09-24 | Clariant Int Ltd | Método para una expresión selectiva independiente de la fuente de carbono de secuencias que codifican una proteína en una célula de hongo filamentoso |
EP3516056A1 (de) | 2016-09-23 | 2019-07-31 | DSM IP Assets B.V. | Guide-rna-expressionssystem für eine wirtszelle |
WO2018114938A1 (en) | 2016-12-21 | 2018-06-28 | Dsm Ip Assets B.V. | Lipolytic enzyme variants |
BR112019012559A2 (pt) | 2016-12-21 | 2019-11-26 | Dsm Ip Assets Bv | variantes de enzima lipolítica |
EP3559222A1 (de) | 2016-12-21 | 2019-10-30 | DSM IP Assets B.V. | Varianten lipolytischer enzyme |
WO2018114912A1 (en) | 2016-12-21 | 2018-06-28 | Dsm Ip Assets B.V. | Lipolytic enzyme variants |
US20200063166A1 (en) | 2017-03-13 | 2020-02-27 | Dsm Ip Assets B.V. | Zinc binuclear cluster transcriptional regulator-deficient strain |
WO2018127611A1 (en) | 2017-04-06 | 2018-07-12 | Dsm Ip Assets B.V. | Self-guiding integration construct (sgic) |
CN112424326A (zh) | 2018-05-07 | 2021-02-26 | 帝斯曼知识产权资产管理有限公司 | 用于酶促油脱胶的方法 |
MX2020014191A (es) | 2018-06-19 | 2021-03-09 | Dsm Ip Assets Bv | Variantes de enzimas lipoliticas. |
US20220389458A1 (en) | 2019-11-04 | 2022-12-08 | Dsm Ip Assets B.V. | Low volume transfection |
JP2021093966A (ja) * | 2019-12-18 | 2021-06-24 | 本田技研工業株式会社 | 糸状菌への遺伝子導入方法及びその利用 |
WO2022269549A2 (en) | 2021-06-24 | 2022-12-29 | Fonterra Co-Operative Group Limited | Recombinant proteins |
WO2023222614A1 (en) | 2022-05-16 | 2023-11-23 | Dsm Ip Assets B.V. | Lipolytic enzyme variants |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DK122686D0 (da) * | 1986-03-17 | 1986-03-17 | Novo Industri As | Fremstilling af proteiner |
EP0429628B1 (de) * | 1989-06-16 | 1998-09-02 | Genencor International, Inc. | Dna-sequenzen, vektoren und fusionspolypeptide zur erhöhung der sekretion gewünschter polypeptide von filamentösen fungi |
DK0481008T3 (da) * | 1989-07-07 | 1998-05-04 | Unilever Plc | Fremgangsmåde til fremstilling af et protein ved anvendelse af en svamp transformeret ved multikopiintegration af en ekspressionsvektor. |
JPH0595787A (ja) * | 1991-10-04 | 1993-04-20 | Jozo Shigen Kenkyusho:Kk | ホスホグリセリン酸キナ−ゼ遺伝子プロモ−タ−、 同タ−ミネ−タ−または/及びこれらからなる 遺伝子発現用ユニツト、並びに、ホスホグリセリ ン酸キナ−ゼのゲノム遺伝子及びcDNA遺伝子 |
MX9206979A (es) * | 1991-12-04 | 1993-07-01 | Novo Nordisk As | Metodo de clonacion de proteinas en levaduras |
JPH05268964A (ja) * | 1992-03-30 | 1993-10-19 | Meiji Milk Prod Co Ltd | 糸状菌プロモーター |
EP1321523A3 (de) * | 1993-07-23 | 2004-03-03 | DSM IP Assets B.V. | Selektionmarker-genfreie rekombinante Stämme: Verfahren zur ihrer Herstellung und die Verwendung dieser Stämme |
JP3638657B2 (ja) * | 1995-03-29 | 2005-04-13 | 株式会社資生堂 | Dna修復酵素 |
HUP9801871A3 (en) * | 1995-04-24 | 1999-12-28 | Chromaxome Corp San Diego | Methods for generating and screening novel metabolic pathways |
JP3343567B2 (ja) * | 1995-06-29 | 2002-11-11 | 大関株式会社 | 糸状菌のエンハンサーdna塩基配列およびその用途 |
DK0873398T3 (da) * | 1996-01-10 | 2005-08-15 | Novozymes As | Fremgangsmåde til in vivo produktion af et mutantbibliotek i celler |
-
1998
- 1998-12-22 AT AT98966868T patent/ATE367439T1/de active
- 1998-12-22 CN CN98812563.3A patent/CN1198924C/zh not_active Expired - Lifetime
- 1998-12-22 BR BR9814404-9A patent/BR9814404A/pt not_active Application Discontinuation
- 1998-12-22 PL PL98341760A patent/PL341760A1/xx unknown
- 1998-12-22 EP EP07112086A patent/EP1854890A1/de not_active Withdrawn
- 1998-12-22 JP JP2000525536A patent/JP4410413B2/ja not_active Expired - Fee Related
- 1998-12-22 DK DK98966868T patent/DK1042461T3/da active
- 1998-12-22 AU AU25151/99A patent/AU736111C/en not_active Ceased
- 1998-12-22 ES ES98966868T patent/ES2287989T3/es not_active Expired - Lifetime
- 1998-12-22 WO PCT/EP1998/008577 patent/WO1999032617A2/en active IP Right Grant
- 1998-12-22 CA CA2313445A patent/CA2313445C/en not_active Expired - Fee Related
- 1998-12-22 EP EP98966868A patent/EP1042461B1/de not_active Revoked
- 1998-12-22 DE DE69838106T patent/DE69838106T2/de not_active Expired - Lifetime
-
2001
- 2001-11-05 US US09/993,164 patent/US20020155536A1/en active Pending
-
2005
- 2005-05-25 JP JP2005152703A patent/JP2005261438A/ja active Pending
-
2009
- 2009-10-20 JP JP2009241780A patent/JP2010046080A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
US20020155536A1 (en) | 2002-10-24 |
JP2010046080A (ja) | 2010-03-04 |
AU736111B2 (en) | 2001-07-26 |
EP1042461A2 (de) | 2000-10-11 |
DE69838106D1 (de) | 2007-08-30 |
ES2287989T3 (es) | 2007-12-16 |
JP2005261438A (ja) | 2005-09-29 |
EP1042461B1 (de) | 2007-07-18 |
WO1999032617A3 (en) | 1999-09-10 |
WO1999032617A2 (en) | 1999-07-01 |
DK1042461T3 (da) | 2007-09-24 |
AU2515199A (en) | 1999-07-12 |
DE69838106T2 (de) | 2008-04-03 |
PL341760A1 (en) | 2001-05-07 |
JP2002509698A (ja) | 2002-04-02 |
AU736111C (en) | 2004-02-19 |
EP1854890A1 (de) | 2007-11-14 |
JP4410413B2 (ja) | 2010-02-03 |
CA2313445A1 (en) | 1999-07-01 |
CN1283227A (zh) | 2001-02-07 |
CA2313445C (en) | 2012-03-20 |
BR9814404A (pt) | 2000-10-10 |
CN1198924C (zh) | 2005-04-27 |
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