DE69736667T2 - Verfahren zum nachweis und amplifikation von nukleinsäuresequenzen unter verbrauch von modifizierten oligonukleotiden mit erhöhter zielschmelztemperatur (tm) - Google Patents

Verfahren zum nachweis und amplifikation von nukleinsäuresequenzen unter verbrauch von modifizierten oligonukleotiden mit erhöhter zielschmelztemperatur (tm) Download PDF

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Publication number
DE69736667T2
DE69736667T2 DE69736667T DE69736667T DE69736667T2 DE 69736667 T2 DE69736667 T2 DE 69736667T2 DE 69736667 T DE69736667 T DE 69736667T DE 69736667 T DE69736667 T DE 69736667T DE 69736667 T2 DE69736667 T2 DE 69736667T2
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probe
oligonucleotide
hybridization
target
oligonucleotides
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DE69736667D1 (de
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McClellan Michael San Diego BECKER
Mehrdad San Diego MAJLESSI
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Gen Probe Inc
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Gen Probe Inc
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
DE69736667T 1996-07-16 1997-07-15 Verfahren zum nachweis und amplifikation von nukleinsäuresequenzen unter verbrauch von modifizierten oligonukleotiden mit erhöhter zielschmelztemperatur (tm) Expired - Lifetime DE69736667T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US2181896P 1996-07-16 1996-07-16
US21818P 1996-07-16
PCT/US1997/012347 WO1998002582A2 (en) 1996-07-16 1997-07-15 Methods for detecting and amplifying nucleic acid sequences using modified oligonucleotides having increased target specific t¿m?

Publications (2)

Publication Number Publication Date
DE69736667D1 DE69736667D1 (de) 2006-10-26
DE69736667T2 true DE69736667T2 (de) 2007-09-06

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DE69736667T Expired - Lifetime DE69736667T2 (de) 1996-07-16 1997-07-15 Verfahren zum nachweis und amplifikation von nukleinsäuresequenzen unter verbrauch von modifizierten oligonukleotiden mit erhöhter zielschmelztemperatur (tm)

Country Status (11)

Country Link
US (3) US6130038A (OSRAM)
EP (1) EP0912767B1 (OSRAM)
JP (2) JP2000514307A (OSRAM)
KR (1) KR20000023814A (OSRAM)
AT (1) ATE339514T1 (OSRAM)
AU (1) AU726821B2 (OSRAM)
CA (1) CA2260749A1 (OSRAM)
DE (1) DE69736667T2 (OSRAM)
DK (1) DK0912767T3 (OSRAM)
ES (1) ES2270467T3 (OSRAM)
WO (1) WO1998002582A2 (OSRAM)

Families Citing this family (100)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5714380A (en) 1986-10-23 1998-02-03 Amoco Corporation Closed vessel for isolating target molecules and for performing amplification
US7070925B1 (en) 1996-07-16 2006-07-04 Gen-Probe Incorporated Method for determining the presence of an RNA analyte in a sample using a modified oligonucleotide probe
AU726821B2 (en) * 1996-07-16 2000-11-23 Gen-Probe Incorporated Methods for detecting and amplifying nucleic acid sequences using modified oligonucleotides having increased target specific Tm
DE69840669D1 (de) 1997-04-10 2009-04-30 Stichting Katholieke Univ Pca3, pca3-gene und verfahren zu ihrer verwendung
WO2000001850A2 (en) 1998-07-02 2000-01-13 Gen-Probe Incorporated Molecular torches
JP2002521070A (ja) 1998-07-31 2002-07-16 ジェン−プローブ・インコーポレーテッド 可逆的阻害プローブ
US6087112A (en) 1998-12-30 2000-07-11 Oligos Etc. Inc. Arrays with modified oligonucleotide and polynucleotide compositions
WO2000070093A1 (en) * 1999-05-13 2000-11-23 Oligos Etc. Inc. Arrays with modified oligonucleotide and polynucleotide compositions
AU7742100A (en) * 1999-09-29 2001-04-30 Oligos Etc. Inc. Arrays with modified oligonucleotide and polynucleotide compositions
US7368545B1 (en) 1999-09-29 2008-05-06 Diagnocure Inc. PCA3 messenger RNA species in benign and malignant prostate tissues
US6844151B1 (en) 1999-09-29 2005-01-18 Oligos Etc. Inc. Methods for production of arrays with modified oligonucleotide and polynucleotide compositions
GB2355791B (en) * 1999-10-28 2004-10-20 Molecular Light Tech Res Ltd Detection of mRNA expression using chemiluminescent labelled probes
US6924108B2 (en) * 1999-12-21 2005-08-02 Ingeneus Corporation Nucleic acid binding enhancement by conjugation with nucleotides, nucleosides, bases and/or their analogues
US7833715B1 (en) * 2000-01-11 2010-11-16 Nanogen Recognomics Gmbh Biomolecules having multiple attachment moieties for binding to a substrate surface
JP2004500119A (ja) 2000-03-31 2004-01-08 メルク　エンド　カムパニー　インコーポレーテッド デルマセンター・ヴァリァビリスｇａｂａ依存性塩素イオンチャンネル
JP4950414B2 (ja) * 2000-08-03 2012-06-13 独立行政法人産業技術総合研究所 核酸の測定方法、それに用いる核酸プローブ及びその方法によって得られるデータを解析する方法
AU7104200A (en) 2000-09-01 2002-03-22 Gen Probe Inc Amplification of hiv-1 sequences for detection of sequences associated with drug-resistance mutations
WO2002022890A2 (en) * 2000-09-12 2002-03-21 Gen-Probe Incorporated Compositions, methods and kits for determining the presence of cryptosporidium organisms in a test sample
DE60115811T2 (de) * 2000-10-25 2006-08-03 Roche Diagnostics Gmbh Amplifizierung unter Verwendung von modifizierten Primern
EP1251183A3 (en) * 2001-04-18 2003-12-10 Exiqon A/S Improved helper probes for detection of a target sequence by a capture oligonucleotide
EP1588139A4 (en) 2001-05-31 2006-10-04 Merck & Co Inc Rhesus monkey, dog and ferret melanin-concentrating hormone type 2 receptor
JP2005517381A (ja) * 2001-06-12 2005-06-16 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア Ｗｉｔ３．０、軟組織創傷治癒を制御する新規遺伝子
US20030165859A1 (en) * 2001-10-23 2003-09-04 Invitrogen Corporation Primers and methods for the detection and discrimination of nucleic acids
US20040009506A1 (en) * 2002-03-29 2004-01-15 Genentech, Inc. Methods and compositions for detection and quantitation of nucleic acid analytes
US7211382B2 (en) * 2002-04-09 2007-05-01 Orchid Cellmark Inc. Primer extension using modified nucleotides
US7199107B2 (en) * 2002-05-23 2007-04-03 Isis Pharmaceuticals, Inc. Antisense modulation of kinesin-like 1 expression
US20030235827A1 (en) * 2002-06-25 2003-12-25 Orchid Biosciences, Inc. Methods and compositions for monitoring primer extension and polymorphism detection reactions
CN100354298C (zh) * 2002-06-28 2007-12-12 兰华生物科技公司 使用单核苷酸多态性组分析受损样品的方法和组合物
WO2004013346A2 (en) * 2002-08-02 2004-02-12 Orchid Biosciences, Inc. Methods and compositions for genotyping
US7192700B2 (en) * 2002-12-20 2007-03-20 Orchid Cellmark Inc. Methods and compositions for conducting primer extension and polymorphism detection reactions
US7319041B2 (en) * 2002-09-27 2008-01-15 Siemens Medical Solutions Diagnostic Applications of acridinium compounds and derivatives in homogeneous assays
WO2004067726A2 (en) * 2003-01-29 2004-08-12 Keck Graduate Institute Isothermal reactions for the amplification of oligonucleotides
ES2427853T3 (es) 2003-02-07 2013-11-04 Diagnocure Inc. Procedimiento para detectar cáncer de próstata en una muestra
AU2004276722B2 (en) 2003-05-19 2009-03-12 Gen-Probe Incorporated Compositions, methods and kits for determining the presence of trichomonas vaginalis in a test sample
WO2005083114A1 (en) * 2004-02-26 2005-09-09 Thomsen Bioscience A/S Method, kit and system for enhanced nested pcr
JP5020818B2 (ja) * 2004-07-01 2012-09-05 ジェン−プローブ・インコーポレーテッド 生物学的試料中の核酸を検出するための方法及び組成物
US7713697B2 (en) * 2004-08-27 2010-05-11 Gen-Probe Incorporated Methods and kits for amplifying DNA
CA2577122C (en) 2004-08-27 2017-06-13 Gen-Probe Incorporated Single-primer nucleic acid amplification methods
CA2491067A1 (en) 2004-12-24 2006-06-24 Stichting Katholieke Universiteit Mrna rations in urinary sediments and/or urine as a prognostic marker for prostate cancer
CA2584230C (en) 2005-05-06 2012-11-27 Gen-Probe Incorporated Methods of nucleic acid target capture
AU2006254891B2 (en) * 2005-06-06 2011-04-14 Gen-Probe Incorporated Compositions, methods and kits for determining the presence of Chlamydophila pneumoniae in a test sample
DK2017356T3 (da) 2006-06-06 2012-01-30 Gen Probe Inc Mærkede oligonukleotider og anvendelse deraf i fremgangsmåder til amplifikation af nukleinsyrer
WO2008016988A1 (en) * 2006-08-01 2008-02-07 Gen-Probe Incorporated Methods of nonspecific target capture of nucleic acids
AU2007284651B2 (en) 2006-08-09 2014-03-20 Institute For Systems Biology Organ-specific proteins and methods of their use
US8183359B2 (en) * 2007-03-01 2012-05-22 Gen-Probe Incorporated Kits for amplifying DNA
EP1978111B1 (en) 2007-04-02 2013-03-27 Gen-Probe Incorporated Compositions, kits and related methods for the detection and/or monitoring of Pseudomonas aeruginosa
US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
EP2238264B1 (en) 2007-12-21 2012-08-01 Gen-Probe Incorporated Detection of antibiotic-resistant microorganisms
US20090215050A1 (en) * 2008-02-22 2009-08-27 Robert Delmar Jenison Systems and methods for point-of-care amplification and detection of polynucleotides
US9434988B2 (en) * 2008-04-30 2016-09-06 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
EP3150727B1 (en) 2008-04-30 2019-07-10 Integrated DNA Technologies Inc. Rnase-h-based assays utilizing modified rna monomers
US8911948B2 (en) * 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
WO2009140374A2 (en) 2008-05-13 2009-11-19 Gen-Probe Incorporated Inactivatable target capture oligomers for use in the selective hybridization and capture of target nucleic acid sequences
EP2297358A2 (en) 2008-05-30 2011-03-23 Gen-Probe Incorporated Compositions, kits and related methods for the detection and/or monitoring of salmonella
JP2012509675A (ja) 2008-11-25 2012-04-26 ジェン−プロウブ インコーポレイテッド 低分子ｒｎａを検出するための組成物および方法、ならびにその使用
SG171914A1 (en) 2008-12-02 2011-07-28 Chiralgen Ltd Method for the synthesis of phosphorus atom modified nucleic acids
EP2910647A1 (en) 2008-12-30 2015-08-26 Gen-Probe Incorporated Compositions, kits and related methods for the detection and/or monitoring of listeria
WO2010126913A1 (en) 2009-04-27 2010-11-04 Gen-Probe Incorporated Methods and kits for use in the selective amplification of target sequences
IN2012DN00720A (OSRAM) 2009-07-06 2015-06-19 Ontorii Inc
AU2010286368B2 (en) 2009-08-31 2015-09-03 Gen-Probe Incorporated Dengue virus assay
CA2896129C (en) 2010-01-22 2015-12-29 Gen-Probe Incorporated Probes for detecting the presence of trichomonas vaginalis in a sample
EP2588629B1 (en) 2010-06-30 2017-05-17 Gen-Probe Incorporated Method and apparatus for identifying analyte-containing samples using single-read determination of analyte and process control signals
US12043866B2 (en) 2010-08-13 2024-07-23 Envirologix Inc. Compositions and methods for quantifying a nucleic acid sequence in a sample
WO2012039448A1 (ja) 2010-09-24 2012-03-29 株式会社キラルジェン 不斉補助基
MX347361B (es) 2011-07-19 2017-04-12 Wave Life Sciences Ltd Metodos para la sintesis de acidos nucleicos funcionalizados.
EP2758547B1 (en) 2011-09-21 2015-09-16 Gen-Probe Incorporated Methods for amplifying nucleic acid using tag-mediated displacement
US9352312B2 (en) 2011-09-23 2016-05-31 Alere Switzerland Gmbh System and apparatus for reactions
NZ701145A (en) 2012-04-09 2016-09-30 Envirologix Inc Compositions and methods for quantifying a nucleic acid sequence in a sample
EP3301188B1 (en) * 2012-04-16 2020-10-07 Life Technologies Corporation Methods for the preparation of rna libraries
EP2872485B1 (en) 2012-07-13 2020-12-16 Wave Life Sciences Ltd. Asymmetric auxiliary group
EP2872147B1 (en) 2012-07-13 2022-12-21 Wave Life Sciences Ltd. Method for making chiral oligonucleotides
AU2013287630B2 (en) 2012-07-13 2017-05-25 Shin Nippon Biomedical Laboratories, Ltd. Chiral nucleic acid adjuvant
AU2013202793B2 (en) 2012-07-31 2014-09-18 Gen-Probe Incorporated System, method and apparatus for automated incubation
WO2014140167A1 (en) 2013-03-14 2014-09-18 Dsm Ip Assets B.V. Cell wall deconstruction enzymes of malbranchea cinnamomea and uses thereof
WO2014140165A1 (en) 2013-03-14 2014-09-18 Dsm Ip Assets B.V. Cell wall deconstruction enzymes of paecilomyces byssochlamydoides and uses thereof
US9347095B2 (en) 2013-03-15 2016-05-24 Bio-Rad Laboratories, Inc. Digital assays for mutation detection
DK3066219T3 (en) 2013-11-08 2019-03-11 Ionis Pharmaceuticals Inc METHODS FOR DETECTING OIGONUCLEOTIDES
EP3095460A4 (en) 2014-01-15 2017-08-23 Shin Nippon Biomedical Laboratories, Ltd. Chiral nucleic acid adjuvant having anti-allergic activity, and anti-allergic agent
US10144933B2 (en) 2014-01-15 2018-12-04 Shin Nippon Biomedical Laboratories, Ltd. Chiral nucleic acid adjuvant having immunity induction activity, and immunity induction activator
JPWO2015108048A1 (ja) 2014-01-15 2017-03-23 株式会社新日本科学 抗腫瘍作用を有するキラル核酸アジュバンド及び抗腫瘍剤
CN106068325B (zh) 2014-01-16 2021-07-09 波涛生命科学有限公司 手性设计
EP3134551B1 (en) 2014-04-22 2020-06-03 Envirologix Inc. Compositions and methods for enhancing and/or predicting dna amplification
BR112017008082A2 (pt) 2014-10-20 2017-12-26 Envirologix Inc composições e métodos para detectar um vírus de rna
KR102657306B1 (ko) 2014-12-08 2024-04-12 버그 엘엘씨 전립선암의 진단 및 치료에서 필라민을 포함하는 마커의 용도
CN114250274A (zh) 2015-04-24 2022-03-29 阿提拉生物系统公司 利用有限核苷酸组成的引物扩增
US10329601B2 (en) * 2015-12-28 2019-06-25 Ionian Technologies, Inc. Nicking and extension amplification reaction (NEAR) of Streptococcus species
US12460254B2 (en) 2018-08-08 2025-11-04 Gen-Probe Incorporated Compositions, methods and kits for detecting Mycoplasma genitalium
WO2020154513A1 (en) 2019-01-25 2020-07-30 Gen-Probe Incorporated Detection of drug-resistant mycoplasma genitalium
AU2020265250A1 (en) 2019-04-30 2021-11-18 Larimar Therapeutics, Inc. Frataxin-sensitive markers for determining effectiveness of frataxin replacement therapy
AU2020299621B2 (en) 2019-07-03 2025-10-30 Gen-Probe Incorporated Oligonucleotides for use in determining the presence of Trichomonas vaginalis in a sample.
CA3152309A1 (en) 2019-08-23 2021-03-04 Gen-Probe Incorporated Compositions, methods and kits for detecting treponema pallidum
US20230243001A1 (en) 2020-07-17 2023-08-03 Gen-Probe Incorporated Detection of Macrolide-Resistant Mycoplasma Genitalium
WO2022047359A1 (en) 2020-08-31 2022-03-03 Berg Llc Protein biomarkers for pancreatic cancer
US20230059578A1 (en) 2021-04-06 2023-02-23 Berg Llc Protein markers for estrogen receptor (er)-positive-like and estrogen receptor (er)-negative-like breast cancer
US20240402177A1 (en) 2021-04-06 2024-12-05 Bpgbio, Inc. Protein markers for estrogen receptor (er)-positive luminal a (la)-like and luminal b1 (lb1)-like breast cancer
WO2022216798A1 (en) 2021-04-06 2022-10-13 Berg Llc Protein markers for the prognosis of breast cancer progression
WO2023196937A1 (en) 2022-04-06 2023-10-12 Larimar Therapeutics, Inc. Frataxin-sensitive markers for monitoring frataxin replacement therapy
WO2023240201A1 (en) 2022-06-08 2023-12-14 Larimar Therapeutics, Inc. Frataxin-sensitive markers for monitoring progression and treatment of leigh syndrome
WO2024220373A1 (en) 2023-04-15 2024-10-24 Accent Therapeutics, Inc. Assays for monitoring inhibition of rna helicase dhx9
WO2025155991A2 (en) 2024-01-19 2025-07-24 Bpgbio, Inc. Predictive biomarkers for response to coenzyme q10 treatment in pancreatic cancer

Family Cites Families (66)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5087558A (en) 1981-09-25 1992-02-11 Webster John A Jr Method for identifying and characterizing organisms
US4766062A (en) * 1984-05-07 1988-08-23 Allied Corporation Displacement polynucleotide assay method and polynucleotide complex reagent therefor
US4820630A (en) * 1984-11-23 1989-04-11 Digene Diagnostics, Incorporated Assay for nucleic acid sequences, particularly genetic lesions, using interactive labels
JPS61243182A (ja) * 1985-04-19 1986-10-29 Nitsukan Kogyo Kk 金属メツキされたチタン酸カリウム繊維およびその製造方法
US4822733A (en) * 1985-05-28 1989-04-18 Amoco Corporation Lifetime-resolved assay procedures
US4797355A (en) * 1985-06-13 1989-01-10 Amgen Inc. Methods for attaching polynucleotides to supports
US4996143A (en) * 1985-12-23 1991-02-26 Syngene, Inc. Fluorescent stokes shift probes for polynucleotide hybridization
EP0232967B1 (en) * 1986-01-10 1993-04-28 Amoco Corporation Competitive homogeneous assay
US4925785A (en) * 1986-03-07 1990-05-15 Biotechnica Diagnostics, Inc. Nucleic acid hybridization assays
US5091519A (en) * 1986-05-01 1992-02-25 Amoco Corporation Nucleotide compositions with linking groups
WO1989001050A1 (en) 1987-07-31 1989-02-09 The Board Of Trustees Of The Leland Stanford Junior University Selective amplification of target polynucleotide sequences
US5030557A (en) * 1987-11-24 1991-07-09 Ml Technology Venture Means and method for enhancing nucleic acid hybridization
US5403711A (en) 1987-11-30 1995-04-04 University Of Iowa Research Foundation Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved
US5118801A (en) * 1988-09-30 1992-06-02 The Public Health Research Institute Nucleic acid process containing improved molecular switch
CA2008096A1 (en) * 1989-01-19 1990-07-19 Samuel Rose Nucleic acid amplification using single primer
CA2031536A1 (en) * 1989-04-05 1990-10-06 Kevin R. Kearney Hybridization promotion reagents
IE81145B1 (en) 1989-04-20 2000-05-03 Thomas Gerard Barry Generation of specific probes for target nucleotide sequences
EP0426843B1 (en) * 1989-05-24 1995-09-13 Amoco Corporation Nucleic acid probes for the detection of neisseria gonorrhoeae
US5849504A (en) 1989-08-11 1998-12-15 Amoco Corporation Nucleic acid probes and methods for detecting Pneumocystis carinii
US5134066A (en) * 1989-08-29 1992-07-28 Monsanto Company Improved probes using nucleosides containing 3-dezauracil analogs
CA2025178A1 (en) * 1989-10-02 1991-04-03 William G. Weisburg Nucleic acid probes for the detection of lyme disease spirochetes
JPH05503011A (ja) * 1989-12-01 1993-05-27 ザ　ボード　オブ　トラスティーズ　オブ　リーランド　スタンフォード　ジュニア　ユニバーシティ 高特異性分子集成体の促進
US5914396A (en) 1990-01-11 1999-06-22 Isis Pharmaceuticals, Inc. 2'-O-modified nucleosides and phosphoramidites
US5536638A (en) 1990-04-18 1996-07-16 N.V. Innogenetics S.A. Hybridization probes derived from the spacer region between the 16S and 23S rRNA genes for the detection of Neisseria gonorrhoeae
US5811533A (en) 1990-06-11 1998-09-22 Nexstar Pharmaceuticals, Inc. High-affinity oligonucleotide ligands to vascular endothelial growth factor (VEGF)
US5614617A (en) * 1990-07-27 1997-03-25 Isis Pharmaceuticals, Inc. Nuclease resistant, pyrimidine modified oligonucleotides that detect and modulate gene expression
US5386023A (en) * 1990-07-27 1995-01-31 Isis Pharmaceuticals Backbone modified oligonucleotide analogs and preparation thereof through reductive coupling
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
EP0513397B1 (en) * 1990-11-29 1999-04-14 Kumiai Chemical Industry Co., Ltd. Benzohydroximoylazole derivative, production thereof and insecticide
US5641625A (en) * 1992-05-22 1997-06-24 Isis Pharmaceuticals, Inc. Cleaving double-stranded DNA with peptide nucleic acids
US5435248A (en) * 1991-07-09 1995-07-25 The Ensign-Bickford Company Extended range digital delay detonator
JP3739785B2 (ja) * 1991-11-26 2006-01-25 アイシス ファーマシューティカルズ，インコーポレイティド 修飾されたピリミジンを含有するオリゴマーを使用する増強された三重らせんおよび二重らせんの成形
DK0618925T4 (da) * 1991-12-24 2012-07-09 Isis Pharmaceuticals Inc Antisense-oligonukleotider
US5700922A (en) * 1991-12-24 1997-12-23 Isis Pharmaceuticals, Inc. PNA-DNA-PNA chimeric macromolecules
US5652099A (en) * 1992-02-12 1997-07-29 Conrad; Michael J. Probes comprising fluorescent nucleosides and uses thereof
DE69207580T2 (de) * 1992-03-23 1996-08-08 Hoffmann La Roche Verfahren zum DNS-Nachweis
EP0651759B1 (en) * 1992-07-23 2003-12-10 Isis Pharmaceuticals, Inc. Novel 2'-o-alkyl nucleosides and phosphoramidites processes for the preparation and uses thereof
US5445933A (en) * 1992-09-15 1995-08-29 Boehringer Mannheim Corporation Strand displacement assay and complex useful therefor
EP0601889A2 (en) * 1992-12-10 1994-06-15 Maine Medical Center Research Institute Nucleic acid probes
WO1994019023A1 (en) * 1993-02-19 1994-09-01 Isis Pharmaceuticals, Inc. Cyclobutyl antisense oligonucleotides, methods of making and use thereof
CA2105364A1 (en) * 1993-09-01 1995-03-02 Eric T. Kool Stem-loop oligonucleotides containing parallel and antiparallel binding domains
US6156501A (en) 1993-10-26 2000-12-05 Affymetrix, Inc. Arrays of modified nucleic acid probes and methods of use
US5925517A (en) * 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5827648A (en) * 1995-12-13 1998-10-27 Chiron Corporation Differential hybridization for relative quantification of variant populations
SE9400522D0 (sv) * 1994-02-16 1994-02-16 Ulf Landegren Method and reagent for detecting specific nucleotide sequences
WO1995032305A1 (en) * 1994-05-19 1995-11-30 Dako A/S Pna probes for detection of neisseria gonorrhoeae and chlamydia trachomatis
ZA956776B (en) * 1994-08-25 1996-03-20 Akzo Nobel Nv A process for making the product of a nucleic acid amplification reaction incapable of being a target for further amplification a diagnostic assay employing said process a kit and a container suitable for carrying out said process for assay
US5656427A (en) * 1994-08-29 1997-08-12 Gen-Probe Incorporated Nucleic acid hybridization assay probes, helper probes and amplification oligonucleotides targeted to Mycoplasma pneumoniae nucleic acid
US5681702A (en) * 1994-08-30 1997-10-28 Chiron Corporation Reduction of nonspecific hybridization by using novel base-pairing schemes
US6001564A (en) * 1994-09-12 1999-12-14 Infectio Diagnostic, Inc. Species specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial pathogens and associated antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories
US5514551A (en) * 1994-10-14 1996-05-07 Gen-Probe Incorporated Compositions for the detection of Chlamydia trachomatis
EP0826066B1 (en) * 1995-05-05 2000-09-13 The Perkin-Elmer Corporation Methods and reagents for combined pcr amplification and hybridization probing assay
DE69638318D1 (de) 1995-06-07 2011-02-17 Gilead Sciences Inc Nukleinsäureliganden, die an DNA-Polymerasen binden und diese inhibieren
US5731148A (en) 1995-06-07 1998-03-24 Gen-Probe Incorporated Adduct protection assay
US5747252A (en) * 1995-06-07 1998-05-05 Gen-Probe Incorporated Nucleic acid probes and amplification oligonucleotides for Neisseria species
JP3898228B2 (ja) * 1996-04-12 2007-03-28 ザ パブリック ヘルス リサーチ インスティチュート オブ ザ シティー オブ ニューヨーク インク 検出プローブ、キット及びアッセイ
US6218107B1 (en) 1996-05-22 2001-04-17 Gen-Probe Incorporated Compositions and methods for detecting the presence of Mycobacterium kansassii
US5898031A (en) * 1996-06-06 1999-04-27 Isis Pharmaceuticals, Inc. Oligoribonucleotides for cleaving RNA
AU726821B2 (en) * 1996-07-16 2000-11-23 Gen-Probe Incorporated Methods for detecting and amplifying nucleic acid sequences using modified oligonucleotides having increased target specific Tm
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6117635A (en) * 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US7070925B1 (en) * 1996-07-16 2006-07-04 Gen-Probe Incorporated Method for determining the presence of an RNA analyte in a sample using a modified oligonucleotide probe
US6747252B2 (en) * 1996-11-15 2004-06-08 Kenneth J. Herzog Multiple head induction sealer apparatus and method
US5846726A (en) * 1997-05-13 1998-12-08 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
US6210015B1 (en) * 1999-10-04 2001-04-03 Kenneth Alan Ross Stand for cakes or pastry

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US6903206B1 (en) 2005-06-07
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ES2270467T3 (es) 2007-04-01
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JP4527789B2 (ja) 2010-08-18
US20050106610A1 (en) 2005-05-19
AU726821B2 (en) 2000-11-23
ATE339514T1 (de) 2006-10-15
DE69736667D1 (de) 2006-10-26
WO1998002582A2 (en) 1998-01-22
US6130038A (en) 2000-10-10
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