DE69322591T2 - Verfahren zur Reinigung von Amylase - Google Patents

Verfahren zur Reinigung von Amylase

Info

Publication number
DE69322591T2
DE69322591T2 DE69322591T DE69322591T DE69322591T2 DE 69322591 T2 DE69322591 T2 DE 69322591T2 DE 69322591 T DE69322591 T DE 69322591T DE 69322591 T DE69322591 T DE 69322591T DE 69322591 T2 DE69322591 T2 DE 69322591T2
Authority
DE
Germany
Prior art keywords
purified
amylase
relates
present
precipitation agent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69322591T
Other languages
English (en)
Other versions
DE69322591D1 (de
Inventor
Ivan C Good
Chong Y Kim
Jayarama K Shetty
Kent M Sproat
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Genencor International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor International Inc filed Critical Genencor International Inc
Publication of DE69322591D1 publication Critical patent/DE69322591D1/de
Application granted granted Critical
Publication of DE69322591T2 publication Critical patent/DE69322591T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/14—Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Nutrition Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Chemical Or Physical Treatment Of Fibers (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Ultra Sonic Daignosis Equipment (AREA)
  • Measuring And Recording Apparatus For Diagnosis (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Detergent Compositions (AREA)
  • Peptides Or Proteins (AREA)
DE69322591T 1992-10-20 1993-09-28 Verfahren zur Reinigung von Amylase Expired - Lifetime DE69322591T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/963,542 US5281526A (en) 1992-10-20 1992-10-20 Method of purification of amylase by precipitation with a metal halide and 4-hydroxybenzic acid or a derivative thereof

Publications (2)

Publication Number Publication Date
DE69322591D1 DE69322591D1 (de) 1999-01-28
DE69322591T2 true DE69322591T2 (de) 1999-05-12

Family

ID=25507369

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69322591T Expired - Lifetime DE69322591T2 (de) 1992-10-20 1993-09-28 Verfahren zur Reinigung von Amylase

Country Status (10)

Country Link
US (1) US5281526A (de)
EP (1) EP0594235B1 (de)
JP (1) JPH06197758A (de)
AT (1) ATE174623T1 (de)
BR (1) BR9304293A (de)
CA (1) CA2108699C (de)
DE (1) DE69322591T2 (de)
DK (1) DK0594235T3 (de)
FI (1) FI934622A (de)
MX (1) MX9306478A (de)

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US20030189215A1 (en) * 2002-04-09 2003-10-09 Jong-Lam Lee Method of fabricating vertical structure leds
JP2008546396A (ja) * 2005-06-24 2008-12-25 ノボザイムス アクティーゼルスカブ 薬学的に使用するためのアミラーゼ
HUE028879T2 (en) * 2006-07-13 2017-01-30 Dsm Ip Assets Bv Use of bacterial amylases in feed for bovine animals
RU2459867C2 (ru) * 2006-12-21 2012-08-27 ДАНИСКО ЮЭс, ИНК., ДЖЕНЕНКОР ДИВИЖН КОМПОЗИЦИИ НА ОСНОВЕ ПОЛИПЕПТИДА α-АМИЛАЗЫ ИЗ BACILLUS, ВИД 195, И ИХ ПРИМЕНЕНИЕ
MX2009009378A (es) * 2007-03-09 2009-09-22 Danisco Us Inc Genencor Div Variantes de alfa-amilasa de especies de bacillus alcalifilico, composiciones que comprenden las variantes de alfa-amilasa, y metodos de uso.
US20080300790A1 (en) * 2007-05-29 2008-12-04 James Kirunda Kakaire Environmental data delivery - edd
CN102131924B (zh) * 2008-06-06 2017-06-09 丹尼斯科美国公司 来自枯草芽孢杆菌的变体α‑淀粉酶及其使用方法
EP2310521A2 (de) * 2008-06-06 2011-04-20 Danisco US Inc. Glucoseherstellung aus stärke unter verwendung von alpha-amylasen aus bacillus subtilis
JP5599113B2 (ja) * 2008-06-06 2014-10-01 ダニスコ・ユーエス・インク 糖化酵素組成物及びその糖化方法
BRPI0920891B1 (pt) 2008-09-25 2023-01-10 Danisco Us Inc Mistura de alfa-amilase e método para produção de um açúcar fermentável
WO2010104675A1 (en) 2009-03-10 2010-09-16 Danisco Us Inc. Bacillus megaterium strain dsm90-related alpha-amylases, and methods of use, thereof
CN102388131B (zh) 2009-04-08 2014-04-30 丹尼斯科美国公司 盐单胞菌属菌株WDG195相关α-淀粉酶及其使用方法
MX2012001243A (es) 2009-08-07 2012-03-26 Danisco Us Inc Combinacion de alfa-amilasa para procesamiento de almidon y metodo de uso de la misma.
WO2011049945A2 (en) 2009-10-23 2011-04-28 Danisco Us Inc. Methods for reducing blue saccharide
WO2011102933A1 (en) 2010-02-18 2011-08-25 Danisco Us Inc. Amylase from nesterenkonia and methods of use, thereof
BR112014010045B1 (pt) 2011-10-28 2021-12-21 Danisco Us Inc Polipeptídeo de alfa-amilase variante formadoras de malto-hexaose, composição compreendendo o mesmo, célula hospedeira, e métodos para remover uma mancha ou sujeira amilácea, para sacarificar uma composição que compreende amido, produção de uma composição alimentícia de desengomagem de um têxtil
CA2858252A1 (en) 2011-12-22 2013-06-27 Danisco Us Inc. Variant alpha-amylases and methods of use, thereof
CA2870830A1 (en) 2012-05-11 2013-11-14 Danisco Us Inc. Use of alpha-amylase from aspergillus clavatus for saccharification
CN104379737B (zh) 2012-06-08 2018-10-23 丹尼斯科美国公司 对淀粉聚合物具有增强的活性的变体α淀粉酶
BR112015002653A2 (pt) 2012-08-16 2018-06-12 Danisco Us Inc método de produção de glicose a partir de amido empregando a alfa-amilase de aspergillus clavatus e uma pululanase
WO2014081622A1 (en) 2012-11-20 2014-05-30 Danisco Us Inc. Amylase with maltogenic properties
EP2931872B1 (de) 2012-12-11 2018-01-17 Danisco US Inc. Trichoderma-reesei-wirtszellen zur expression einer glukoamylase aus aspergillus fumigatus und verfahren zur verwendung davon
WO2014093125A1 (en) 2012-12-14 2014-06-19 Danisco Us Inc. Method of using alpha-amylase from aspergillus fumigatus and isoamylase for saccharification
WO2014099415A1 (en) 2012-12-20 2014-06-26 Danisco Us Inc. Method of using alpha-amylase from aspergillus terreus and pullulanase for saccharification
WO2014099525A1 (en) 2012-12-21 2014-06-26 Danisco Us Inc. Paenibacillus curdlanolyticus amylase, and methods of use, thereof
EP2935575B1 (de) 2012-12-21 2018-04-18 Danisco US Inc. Alpha-amylase-varianten
WO2014164777A1 (en) 2013-03-11 2014-10-09 Danisco Us Inc. Alpha-amylase combinatorial variants
WO2014200656A1 (en) 2013-06-13 2014-12-18 Danisco Us Inc. Alpha-amylase from streptomyces umbrinus
WO2014200657A1 (en) 2013-06-13 2014-12-18 Danisco Us Inc. Alpha-amylase from streptomyces xiamenensis
WO2014200658A1 (en) 2013-06-13 2014-12-18 Danisco Us Inc. Alpha-amylase from promicromonospora vindobonensis
EP3011020A1 (de) 2013-06-17 2016-04-27 Danisco US Inc. Alpha-amylase aus einem mitglied der bacillaceae-familie
EP3060659B1 (de) 2013-10-03 2019-05-29 Danisco US Inc. Alpha-amylase aus exiguobacterium, und deren verfahren und anwendung
WO2015050724A1 (en) 2013-10-03 2015-04-09 Danisco Us Inc. Alpha-amylases from a subset of exiguobacterium, and methods of use, thereof
EP3071691B1 (de) 2013-11-20 2019-10-23 Danisco US Inc. Variante alpha-amylasen mit verringerter suszeptibilität gegenüber proteasespaltung und verfahren zur verwendung davon
WO2015094809A1 (en) 2013-12-19 2015-06-25 Danisco Us Inc. Chimeric fungal alpha-amylases comprising carbohydrate binding module and the use thereof
WO2015123063A1 (en) 2014-02-13 2015-08-20 Danisco Us Inc. Strategy for sucrose reduction and generation of insoluble fiber in juices
EP3262180A1 (de) 2015-02-25 2018-01-03 Danisco US Inc. Alpha-glucosidase, zusammensetzungen und verfahren
JP2019500058A (ja) 2015-12-09 2019-01-10 ダニスコ・ユーエス・インク α−アミラーゼ組み合わせ変異体
WO2017173190A2 (en) 2016-04-01 2017-10-05 Danisco Us Inc. Alpha-amylases, compositions & methods
WO2017173324A2 (en) 2016-04-01 2017-10-05 Danisco Us Inc. Alpha-amylases, compositions & methods
US20200216787A1 (en) 2017-09-18 2020-07-09 Dupont Nutrition Biosciences Aps Riboflavinase enzymes and their use to prevent off flavor in brewing
MX2020006432A (es) 2017-12-19 2020-10-16 Dupont Nutrition Biosci Aps Modificacion enzimatica mejorada de fosfolipidos en alimentos.
EP3818154A1 (de) 2018-07-06 2021-05-12 DuPont Nutrition Biosciences ApS Xylanase-haltige futterzusatzstoffe für tierfutter auf getreidebasis
JP2022513085A (ja) 2018-11-20 2022-02-07 デュポン ニュートリション バイオサイエンシス エーピーエス 改変ロバスト高Ｔｍ－フィターゼ分岐群ポリペプチド及びその断片
US20230009832A1 (en) 2019-11-20 2023-01-12 Dupont Nutrition Biosciences Aps Thermostable phytase variants
WO2021127360A1 (en) 2019-12-19 2021-06-24 Dupont Nutrition Biosciences Aps Diet formulations
BR112022017034A2 (pt) 2020-02-28 2022-12-13 Dupont Nutrition Biosci Aps Composições de ração
CA3192286A1 (en) 2020-09-21 2022-03-24 Jacob Flyvholm Cramer Combination of nonmaltogenic exoamylase and glucoamylase for improving bread resilience and reducing amount of added sugars
WO2022266456A2 (en) 2021-06-18 2022-12-22 Dupont Nutrition Biosciences Aps Proteases for beer haze reduction
WO2023092018A1 (en) 2021-11-17 2023-05-25 Dupont Nutrition Biosciences Aps Improved enzymatic modification of galactolipids in food
WO2024015974A1 (en) 2022-07-15 2024-01-18 Dupont Nutrition Biosciences Aps Improved enzymatic modification of phospholipids in food

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US2695863A (en) * 1952-04-16 1954-11-30 Purdue Research Foundation Process for preparing alpha amylase
JPS52108B1 (de) * 1971-04-19 1977-01-05
US4676986A (en) * 1983-05-11 1987-06-30 Sills Alexander M Schwanniomyces castellii strains and brewing process
ES526406A0 (es) * 1983-10-11 1985-11-01 Espanola Petrol Procedimiento para producir -amilasas termoestables por cultivo de microorganismos a altas temperaturas
US4717662A (en) * 1985-01-31 1988-01-05 Miles Laboratories, Inc. Thermal stabilization of alpha-amylase
US4659667A (en) * 1985-02-26 1987-04-21 Miles Laboratories, Inc. Process to recover crystalline enzymes and crystalline enzymes produced thereby
US4673647A (en) * 1985-05-06 1987-06-16 Miles Laboratories, Inc. Process to solubilize enzymes and an enzyme liquid product produced thereby
US4728613A (en) * 1985-09-04 1988-03-01 Miles Laboratories, Inc. Method for the recovery of extracellular enzymes from whole fermentation beer
EP0322082A1 (de) * 1987-12-23 1989-06-28 Gist-Brocades N.V. Gereinigtes industrielles Enzym und Verfahren zur Herstellung davon
JP2886921B2 (ja) * 1988-03-18 1999-04-26 ジェネンカー インコーポレイテッド スブチリシンの結晶化法
US5041377A (en) * 1988-03-18 1991-08-20 Genencor International Inc. Subtilisin crystallization process
ES2060360T3 (es) * 1989-12-21 1994-11-16 Novo Nordisk As Metodo para la cristalizacion de enzimas.

Also Published As

Publication number Publication date
ATE174623T1 (de) 1999-01-15
JPH06197758A (ja) 1994-07-19
FI934622A0 (fi) 1993-10-20
US5281526A (en) 1994-01-25
CA2108699A1 (en) 1994-04-21
BR9304293A (pt) 1994-04-26
MX9306478A (es) 1994-06-30
DK0594235T3 (da) 1999-08-23
EP0594235A3 (de) 1995-03-15
EP0594235A2 (de) 1994-04-27
EP0594235B1 (de) 1998-12-16
CA2108699C (en) 2005-03-29
DE69322591D1 (de) 1999-01-28
FI934622A (fi) 1994-04-21

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Legal Events

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8364 No opposition during term of opposition