DE69322591D1 - Verfahren zur Reinigung von Amylase - Google Patents

Verfahren zur Reinigung von Amylase

Info

Publication number
DE69322591D1
DE69322591D1 DE69322591T DE69322591T DE69322591D1 DE 69322591 D1 DE69322591 D1 DE 69322591D1 DE 69322591 T DE69322591 T DE 69322591T DE 69322591 T DE69322591 T DE 69322591T DE 69322591 D1 DE69322591 D1 DE 69322591D1
Authority
DE
Germany
Prior art keywords
purified
amylase
relates
present
precipitation agent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69322591T
Other languages
English (en)
Other versions
DE69322591T2 (de
Inventor
Ivan C Good
Chong Y Kim
Jayarama K Shetty
Kent M Sproat
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Genencor International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor International Inc filed Critical Genencor International Inc
Publication of DE69322591D1 publication Critical patent/DE69322591D1/de
Application granted granted Critical
Publication of DE69322591T2 publication Critical patent/DE69322591T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/14—Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Nutrition Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Chemical Or Physical Treatment Of Fibers (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Ultra Sonic Daignosis Equipment (AREA)
  • Measuring And Recording Apparatus For Diagnosis (AREA)
  • Peptides Or Proteins (AREA)
  • Detergent Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
DE69322591T 1992-10-20 1993-09-28 Verfahren zur Reinigung von Amylase Expired - Lifetime DE69322591T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/963,542 US5281526A (en) 1992-10-20 1992-10-20 Method of purification of amylase by precipitation with a metal halide and 4-hydroxybenzic acid or a derivative thereof

Publications (2)

Publication Number Publication Date
DE69322591D1 true DE69322591D1 (de) 1999-01-28
DE69322591T2 DE69322591T2 (de) 1999-05-12

Family

ID=25507369

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69322591T Expired - Lifetime DE69322591T2 (de) 1992-10-20 1993-09-28 Verfahren zur Reinigung von Amylase

Country Status (10)

Country Link
US (1) US5281526A (de)
EP (1) EP0594235B1 (de)
JP (1) JPH06197758A (de)
AT (1) ATE174623T1 (de)
BR (1) BR9304293A (de)
CA (1) CA2108699C (de)
DE (1) DE69322591T2 (de)
DK (1) DK0594235T3 (de)
FI (1) FI934622A (de)
MX (1) MX9306478A (de)

Families Citing this family (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2213122A1 (en) * 1994-12-28 1996-07-04 Genencor International, Inc. Enzyme stabilization
DE50107849D1 (de) 2000-07-28 2005-12-01 Henkel Kgaa Neues amylolytisches enzym aus bacillus sp. a 7-7 (dsm 12368) sowie wasch- und reinigungsmittel mit diesem neuen amylolytischen enzym
US20030189215A1 (en) * 2002-04-09 2003-10-09 Jong-Lam Lee Method of fabricating vertical structure leds
US8017351B2 (en) * 2005-06-24 2011-09-13 Novozymes A/S Amylases for pharmaceutical use
CN103120264B (zh) * 2006-07-13 2015-07-08 诺维信公司 细菌淀粉酶在牛类动物的饲料中的用途
KR20090101193A (ko) * 2006-12-21 2009-09-24 다니스코 유에스 인크. 바실러스 종 １９５ 의 알파-아밀라아제 폴리펩티드에 대한 조성물 및 용도
MX2009009378A (es) 2007-03-09 2009-09-22 Danisco Us Inc Genencor Div Variantes de alfa-amilasa de especies de bacillus alcalifilico, composiciones que comprenden las variantes de alfa-amilasa, y metodos de uso.
US20080300790A1 (en) * 2007-05-29 2008-12-04 James Kirunda Kakaire Environmental data delivery - edd
US9040278B2 (en) * 2008-06-06 2015-05-26 Danisco Us Inc. Production of glucose from starch using alpha-amylases from Bacillus subtilis
WO2009149283A1 (en) * 2008-06-06 2009-12-10 Danisco Us Inc. Saccharification enzyme composition
US8323945B2 (en) 2008-06-06 2012-12-04 Danisco Us Inc. Variant alpha-amylases from Bacillus subtilis and methods of uses, thereof
DK2337837T4 (en) 2008-09-25 2017-02-06 Danisco Us Inc ALPHA-AMYLASE MIXTURES AND PROCEDURES FOR USING IT
CN102341495A (zh) 2009-03-10 2012-02-01 丹尼斯科美国公司 巨大芽孢杆菌菌株DSM90相关的α-淀粉酶及其使用方法
EP2417254B1 (de) 2009-04-08 2014-05-21 Danisco US Inc. Alpha-amylasen in verbindung mit halomonas-stamm wdg195 sowie verfahren zur verwendung davon
CN102647918B (zh) 2009-08-07 2015-05-27 丹尼斯科美国公司 用于淀粉加工的α-淀粉酶混合物及其使用方法
WO2011049945A2 (en) 2009-10-23 2011-04-28 Danisco Us Inc. Methods for reducing blue saccharide
US8815559B2 (en) 2010-02-18 2014-08-26 Danisco Us Inc. Amylase from nesterenkonia and methods of use, thereof
RU2014121491A (ru) 2011-10-28 2015-12-10 ДАНИСКО ЮЭс ИНК. Варианты вариантной альфа-амилазы, образующей мальтогексаозу
IN2014DN03298A (de) 2011-12-22 2015-06-26 Danisco Us Inc
BR112014027275A2 (pt) 2012-05-11 2017-08-08 Danisco Us Inc uso de alfa-amilase de aspergillus clavatus para sacarificação
WO2014007921A1 (en) 2012-06-08 2014-01-09 Danisco Us Inc. Variant alpha amylases with enhanced activity on starch polymers
US20150218606A1 (en) 2012-08-16 2015-08-06 Danisco Us Inc. Method of using alpha-amylase from aspergillus clavatus and pullulanase for saccharification
WO2014081622A1 (en) 2012-11-20 2014-05-30 Danisco Us Inc. Amylase with maltogenic properties
US20160115509A1 (en) 2012-12-11 2016-04-28 Danisco Us Inc. Trichoderma reesei host cells expressing a glucoamylase from aspergillus fumigatus and methods of use thereof
EP2931911A1 (de) 2012-12-14 2015-10-21 Danisco US Inc. Verfahren zur verwendung von alpha-amylase aus aspergillus fumigatus und isoamylase zur verzuckerung
EP2904105A1 (de) 2012-12-20 2015-08-12 Danisco US Inc. Verfahren zur verwendung von alpha-amylase aus aspergillus terreus und pullulanase für eine verzuckerung
EP2935575B1 (de) 2012-12-21 2018-04-18 Danisco US Inc. Alpha-amylase-varianten
WO2014099525A1 (en) 2012-12-21 2014-06-26 Danisco Us Inc. Paenibacillus curdlanolyticus amylase, and methods of use, thereof
US20160017305A1 (en) 2013-03-11 2016-01-21 Danisco Us Inc. Alpha-amylase combinatorial variants
WO2014200656A1 (en) 2013-06-13 2014-12-18 Danisco Us Inc. Alpha-amylase from streptomyces umbrinus
WO2014200658A1 (en) 2013-06-13 2014-12-18 Danisco Us Inc. Alpha-amylase from promicromonospora vindobonensis
WO2014200657A1 (en) 2013-06-13 2014-12-18 Danisco Us Inc. Alpha-amylase from streptomyces xiamenensis
EP3011020A1 (de) 2013-06-17 2016-04-27 Danisco US Inc. Alpha-amylase aus einem mitglied der bacillaceae-familie
EP3052622B1 (de) 2013-10-03 2018-09-19 Danisco US Inc. Alpha-amylasen aus einem untersatz von exiguobakterium und verfahren zur verwendung davon
DK3060659T3 (da) 2013-10-03 2019-09-09 Danisco Us Inc Alfa-amylaser fra exiguobacterium og fremgangsmåder til anvendelse deraf
JP6560214B2 (ja) 2013-11-20 2019-08-14 ダニスコ・ユーエス・インク プロテアーゼ開裂に対する感受性を低減した変異体α−アミラーゼ、及びその使用方法
WO2015094809A1 (en) 2013-12-19 2015-06-25 Danisco Us Inc. Chimeric fungal alpha-amylases comprising carbohydrate binding module and the use thereof
US20170006902A1 (en) 2014-02-13 2017-01-12 Danisco Us Inc. Strategy for sucrose reduction and generation of insoluble fiber in juices
WO2016138315A1 (en) 2015-02-25 2016-09-01 Danisco Us Inc Alpha-glucosidase, compositions & methods
CN108779448B (zh) 2015-12-09 2023-08-18 丹尼斯科美国公司 α-淀粉酶组合变体
WO2017173324A2 (en) 2016-04-01 2017-10-05 Danisco Us Inc. Alpha-amylases, compositions & methods
WO2017173190A2 (en) 2016-04-01 2017-10-05 Danisco Us Inc. Alpha-amylases, compositions & methods
WO2019055940A1 (en) 2017-09-18 2019-03-21 Dupont Nutrition Biosciences Aps ENZYMES RIBOFLAVINASES AND THEIR USE FOR REMOVING AROMA DURING BREWING
CN111699251A (zh) 2017-12-19 2020-09-22 杜邦营养生物科学有限公司 磷脂在食品中的改善的酶促修饰
MX2021000149A (es) 2018-07-06 2021-03-25 Dupont Nutrition Biosci Aps Aditivos para piensos que contienen xilanasa para piensos para animales a base de cereales.
CA3120360A1 (en) 2018-11-20 2020-05-28 Dupont Nutrition Biosciences Aps Engineered robust high tm-phytase clade polypeptides and fragments thereof
WO2021102238A1 (en) 2019-11-20 2021-05-27 Dupont Nutrition Biosciences Aps Thermostable phytase variants
US20230038728A1 (en) 2019-12-19 2023-02-09 Leon MARCHAL Diet formulations
BR112022017034A2 (pt) 2020-02-28 2022-12-13 Dupont Nutrition Biosci Aps Composições de ração
EP4213632A1 (de) 2020-09-21 2023-07-26 DuPont Nutrition Biosciences ApS Kombination aus nichtmaltogener exoamylase und glucoamylase zur verbesserung der brotelastizität und zur reduzierung der menge an zugesetzten zuckern
EP4355872A2 (de) 2021-06-18 2024-04-24 International N&H Denmark ApS Proteasen zur verminderung der biertrübung
WO2023092018A1 (en) 2021-11-17 2023-05-25 Dupont Nutrition Biosciences Aps Improved enzymatic modification of galactolipids in food
WO2024015974A1 (en) 2022-07-15 2024-01-18 Dupont Nutrition Biosciences Aps Improved enzymatic modification of phospholipids in food
WO2024124013A1 (en) 2022-12-09 2024-06-13 International N&H Denmark Aps Feed formulations comprising a phytase for dairy ruminant animals

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2695863A (en) * 1952-04-16 1954-11-30 Purdue Research Foundation Process for preparing alpha amylase
JPS52108B1 (de) * 1971-04-19 1977-01-05
US4676986A (en) * 1983-05-11 1987-06-30 Sills Alexander M Schwanniomyces castellii strains and brewing process
ES8602115A1 (es) * 1983-10-11 1985-11-01 Espanola Petrol Procedimiento para producir -amilasas termoestables por cultivo de microorganismos a altas temperaturas
US4717662A (en) * 1985-01-31 1988-01-05 Miles Laboratories, Inc. Thermal stabilization of alpha-amylase
US4659667A (en) * 1985-02-26 1987-04-21 Miles Laboratories, Inc. Process to recover crystalline enzymes and crystalline enzymes produced thereby
US4673647A (en) * 1985-05-06 1987-06-16 Miles Laboratories, Inc. Process to solubilize enzymes and an enzyme liquid product produced thereby
US4728613A (en) * 1985-09-04 1988-03-01 Miles Laboratories, Inc. Method for the recovery of extracellular enzymes from whole fermentation beer
JPH02502786A (ja) * 1987-12-23 1990-09-06 ギスト　ブロカデス　ナームローゼ　フェンノートチャップ 精製された工業酵素及びその調製方法
US5041377A (en) * 1988-03-18 1991-08-20 Genencor International Inc. Subtilisin crystallization process
JP2886921B2 (ja) * 1988-03-18 1999-04-26 ジェネンカー インコーポレイテッド スブチリシンの結晶化法
DE69004101T2 (de) * 1989-12-21 1994-03-24 Novonordisk As Verfahren zur kristallisation von enzymen.

Also Published As

Publication number Publication date
FI934622A0 (fi) 1993-10-20
DE69322591T2 (de) 1999-05-12
BR9304293A (pt) 1994-04-26
CA2108699C (en) 2005-03-29
FI934622A (fi) 1994-04-21
DK0594235T3 (da) 1999-08-23
US5281526A (en) 1994-01-25
EP0594235B1 (de) 1998-12-16
MX9306478A (es) 1994-06-30
EP0594235A3 (de) 1995-03-15
JPH06197758A (ja) 1994-07-19
ATE174623T1 (de) 1999-01-15
CA2108699A1 (en) 1994-04-21
EP0594235A2 (de) 1994-04-27

Similar Documents

Publication Publication Date Title
DE69322591D1 (de) Verfahren zur Reinigung von Amylase
KR830005136A (ko) 옥타히드로-1h-인돌-2-카르복실산 치환아실 유도체의 제조방법
DE69412644T2 (de) Verfahren zur herstellung von leukotriene-antagonisten
ATE382344T1 (de) Verfahren zur rückgewinnung von statinverbindungen aus einer fermentationsbrühe
NO20045491L (no) Fremgangsmate for fremstilling av dioksan eddiksyreester
KR860002513A (ko) 세팔로스포린 유도체의 제조방법
NO944215L (no) Fremgangsmåte for stereospesifikk hydrolyse av piperidindion-derivator
CA2187210A1 (en) Method for purifying diacetylrhein
KR870002150A (ko) 알킬카르바모일옥시메틸 세펨화합물의 제조방법
DE69632919D1 (de) Verfahren zur herstellung von 2-chlor-5-chloromethyl-thiazol
ATE384067T1 (de) Verfahren zur herstellung von 3-cephemderivaten
DE69609815T2 (de) Verfahren zur reinigung von pentafluorethan
ATE71383T1 (de) Verfahren zur herstellung von cephalosporinestern.
DE60133395D1 (de) Verfahren zur herstellung von alpha'-chlorketonen
DE69700903D1 (de) Verfahren zur Isolierung eines pharmazeutisch verträglichen Alkalimetallsalzes der Clavulansäure
US3864332A (en) Method of preparing {60 -aminobenzlpenicillin
ATE155132T1 (de) Verfahren zur herstellung von amino- thiadiazolylacetylhaliden
KR890006547A (ko) 용해성이 개량된 α-L-아스파르틸-L-페닐알라닌 메틸에스테르 제조법
KR960701062A (ko) 효소적 아실화 후 세파클로르의 개선된 단리 방법(improved process for isolation of cefaclor after enzymatic acylation)
DE69416117D1 (de) Verfahren zur herstellung von kondensierten carbapenemderivaten
KR960004334A (ko) 신규한 아세틸살리실산 유도체 및 그의 제조방법
ATE61583T1 (de) Verfahren zur herstellung von benzimidazolthiolen.
DE68907146D1 (de) Verfahren zur reinigung von d-hydroxyphenoxypropionsaeure.
EP0417432A1 (de) Verfahren zur Herstellung von 3-Hydroxy-2-ketosäuren
ATE260888T1 (de) Verfahren zur herstellung von optisch aktiven n- geschützten n-methyl-phenylalanin-derivaten

Legal Events

Date Code Title Description
8364 No opposition during term of opposition