MX9306478A - Metodo de purificacion de amilasa, la amilasa purificada asi obtenida en los usos de la imilasa purificada. - Google Patents

Metodo de purificacion de amilasa, la amilasa purificada asi obtenida en los usos de la imilasa purificada.

Info

Publication number
MX9306478A
MX9306478A MX9306478A MX9306478A MX9306478A MX 9306478 A MX9306478 A MX 9306478A MX 9306478 A MX9306478 A MX 9306478A MX 9306478 A MX9306478 A MX 9306478A MX 9306478 A MX9306478 A MX 9306478A
Authority
MX
Mexico
Prior art keywords
purified
amylase
imilase
purification
relates
Prior art date
Application number
MX9306478A
Other languages
English (en)
Inventor
Kent M Sproat
Ivan C Good
Chong Y Kim
Jayarama K Shetty
Original Assignee
Solvay Enzymes Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Solvay Enzymes Inc filed Critical Solvay Enzymes Inc
Publication of MX9306478A publication Critical patent/MX9306478A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/14—Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation

Abstract

El presente invento se refiere a un método para la preparación de una amilasa purificada recuperada de un caldo de fermentación, que comprende la adición de un agente de precipitación que es una sal haluro, más preferiblemente cloruro sódico o potàsico. De acuerdo con una realización preferida, se añade un agente de precipitación adicional que es un compuesto orgánico, más preferiblemente un éster alquílico del ácido 4-hidroxibenzoico. El presente invento también se refiere a las amilasas purificadas así obtenidas y los usos de estas amilasas purificadas.
MX9306478A 1992-10-20 1993-10-19 Metodo de purificacion de amilasa, la amilasa purificada asi obtenida en los usos de la imilasa purificada. MX9306478A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/963,542 US5281526A (en) 1992-10-20 1992-10-20 Method of purification of amylase by precipitation with a metal halide and 4-hydroxybenzic acid or a derivative thereof

Publications (1)

Publication Number Publication Date
MX9306478A true MX9306478A (es) 1994-06-30

Family

ID=25507369

Family Applications (1)

Application Number Title Priority Date Filing Date
MX9306478A MX9306478A (es) 1992-10-20 1993-10-19 Metodo de purificacion de amilasa, la amilasa purificada asi obtenida en los usos de la imilasa purificada.

Country Status (10)

Country Link
US (1) US5281526A (es)
EP (1) EP0594235B1 (es)
JP (1) JPH06197758A (es)
AT (1) ATE174623T1 (es)
BR (1) BR9304293A (es)
CA (1) CA2108699C (es)
DE (1) DE69322591T2 (es)
DK (1) DK0594235T3 (es)
FI (1) FI934622A (es)
MX (1) MX9306478A (es)

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CN102803481A (zh) 2009-10-23 2012-11-28 丹尼斯科美国公司 用于减少蓝色糖的方法
US8815559B2 (en) 2010-02-18 2014-08-26 Danisco Us Inc. Amylase from nesterenkonia and methods of use, thereof
EP3572505A3 (en) 2011-10-28 2019-12-11 Danisco US Inc. Variant maltohexaose-forming alpha-amylase variants
IN2014DN03298A (es) 2011-12-22 2015-06-26 Danisco Us Inc
CA2870830A1 (en) 2012-05-11 2013-11-14 Danisco Us Inc. Use of alpha-amylase from aspergillus clavatus for saccharification
CN104379737B (zh) 2012-06-08 2018-10-23 丹尼斯科美国公司 对淀粉聚合物具有增强的活性的变体α淀粉酶
CN104583412A (zh) 2012-08-16 2015-04-29 丹尼斯科美国公司 采用棒曲霉α-淀粉酶和支链淀粉酶由淀粉制备葡萄糖的方法
US20180112203A1 (en) 2012-11-20 2018-04-26 Danisco Us Inc. Amylase with maltogenic properties
EP2931872B1 (en) 2012-12-11 2018-01-17 Danisco US Inc. Trichoderma reesei host cells expressing a glucoamylase from aspergillus fumigatus and methods of use thereof
CA2894513A1 (en) 2012-12-14 2014-06-19 Danisco Us Inc. Method of using alpha-amylase from aspergillus fumigatus and isoamylase for saccharification
EP2904105A1 (en) 2012-12-20 2015-08-12 Danisco US Inc. Method of using alpha-amylase from aspergillus terreus and pullulanase for saccharification
CN104884614A (zh) 2012-12-21 2015-09-02 丹尼斯科美国公司 α-淀粉酶变体
WO2014099525A1 (en) 2012-12-21 2014-06-26 Danisco Us Inc. Paenibacillus curdlanolyticus amylase, and methods of use, thereof
EP2970929A1 (en) 2013-03-11 2016-01-20 Danisco US Inc. Alpha-amylase combinatorial variants
WO2014200658A1 (en) 2013-06-13 2014-12-18 Danisco Us Inc. Alpha-amylase from promicromonospora vindobonensis
WO2014200656A1 (en) 2013-06-13 2014-12-18 Danisco Us Inc. Alpha-amylase from streptomyces umbrinus
WO2014200657A1 (en) 2013-06-13 2014-12-18 Danisco Us Inc. Alpha-amylase from streptomyces xiamenensis
US20160130571A1 (en) 2013-06-17 2016-05-12 Danisco Us Inc. Alpha-Amylase from Bacillaceae Family Member
US20160186102A1 (en) 2013-10-03 2016-06-30 Danisco Us Inc. Alpha-amylases from exiguobacterium, and methods of use, thereof
US20160160199A1 (en) 2013-10-03 2016-06-09 Danisco Us Inc. Alpha-amylases from exiguobacterium, and methods of use, thereof
BR112016010551A2 (pt) 2013-11-20 2017-12-05 Danisco Us Inc variantes de alfa-amilases tendo suscetibilidade reduzida à clivagem da protease e métodos de uso das mesmas
WO2015094809A1 (en) 2013-12-19 2015-06-25 Danisco Us Inc. Chimeric fungal alpha-amylases comprising carbohydrate binding module and the use thereof
WO2015123063A1 (en) 2014-02-13 2015-08-20 Danisco Us Inc. Strategy for sucrose reduction and generation of insoluble fiber in juices
WO2016138315A1 (en) 2015-02-25 2016-09-01 Danisco Us Inc Alpha-glucosidase, compositions & methods
BR112018011755A2 (pt) 2015-12-09 2018-12-04 Danisco Us Inc variantes combinatórias de alfa-amilase
WO2017173190A2 (en) 2016-04-01 2017-10-05 Danisco Us Inc. Alpha-amylases, compositions & methods
WO2017173324A2 (en) 2016-04-01 2017-10-05 Danisco Us Inc. Alpha-amylases, compositions & methods
WO2019055940A1 (en) 2017-09-18 2019-03-21 Dupont Nutrition Biosciences Aps ENZYMES RIBOFLAVINASES AND THEIR USE FOR REMOVING AROMA DURING BREWING
US11744254B2 (en) 2017-12-19 2023-09-05 Dupont Nutrition Biosciences Aps Enzymatic modification of phospholipids in food
MX2021000149A (es) 2018-07-06 2021-03-25 Dupont Nutrition Biosci Aps Aditivos para piensos que contienen xilanasa para piensos para animales a base de cereales.
JP2022513085A (ja) 2018-11-20 2022-02-07 デュポン ニュートリション バイオサイエンシス エーピーエス 改変ロバスト高Ｔｍ－フィターゼ分岐群ポリペプチド及びその断片
US20230009832A1 (en) 2019-11-20 2023-01-12 Dupont Nutrition Biosciences Aps Thermostable phytase variants
WO2021127360A1 (en) 2019-12-19 2021-06-24 Dupont Nutrition Biosciences Aps Diet formulations
WO2021173974A1 (en) 2020-02-28 2021-09-02 Dupont Nutrition Biosciences Aps Feed compositions
US20230404088A1 (en) 2020-09-21 2023-12-21 Dupont Nutrition Biosciences Aps Combination of nonmaltogenic exoamylase and glucoamylase for improving bread resilience and reducing amount of added sugars
EP4355872A2 (en) 2021-06-18 2024-04-24 International N&H Denmark ApS Proteases for beer haze reduction
WO2023092018A1 (en) 2021-11-17 2023-05-25 Dupont Nutrition Biosciences Aps Improved enzymatic modification of galactolipids in food
WO2024015974A1 (en) 2022-07-15 2024-01-18 Dupont Nutrition Biosciences Aps Improved enzymatic modification of phospholipids in food

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Also Published As

Publication number Publication date
FI934622A0 (fi) 1993-10-20
CA2108699A1 (en) 1994-04-21
BR9304293A (pt) 1994-04-26
FI934622A (fi) 1994-04-21
JPH06197758A (ja) 1994-07-19
US5281526A (en) 1994-01-25
EP0594235A3 (en) 1995-03-15
CA2108699C (en) 2005-03-29
EP0594235B1 (en) 1998-12-16
EP0594235A2 (en) 1994-04-27
DK0594235T3 (da) 1999-08-23
ATE174623T1 (de) 1999-01-15
DE69322591D1 (de) 1999-01-28
DE69322591T2 (de) 1999-05-12

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Legal Events

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FG Grant or registration
MM Annulment or lapse due to non-payment of fees