DE60129432D1 - Verfahren zur rückfaltung von proteinen mit freien cysteinresten - Google Patents
Verfahren zur rückfaltung von proteinen mit freien cysteinrestenInfo
- Publication number
- DE60129432D1 DE60129432D1 DE60129432T DE60129432T DE60129432D1 DE 60129432 D1 DE60129432 D1 DE 60129432D1 DE 60129432 T DE60129432 T DE 60129432T DE 60129432 T DE60129432 T DE 60129432T DE 60129432 D1 DE60129432 D1 DE 60129432D1
- Authority
- DE
- Germany
- Prior art keywords
- proteins
- recycling
- rest
- novel methods
- free cystein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/56—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule
- A61K47/59—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes
- A61K47/60—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes the organic macromolecular compound being a polyoxyalkylene oligomer, polymer or dendrimer, e.g. PEG, PPG, PEO or polyglycerol
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P5/00—Drugs for disorders of the endocrine system
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
- C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/505—Erythropoietin [EPO]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/515—Angiogenesic factors; Angiogenin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/53—Colony-stimulating factor [CSF]
- C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/555—Interferons [IFN]
- C07K14/56—IFN-alpha
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormones [GH] (Somatotropin)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Zoology (AREA)
- Toxicology (AREA)
- Endocrinology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Vascular Medicine (AREA)
- Epidemiology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Diabetes (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US20461700P | 2000-05-16 | 2000-05-16 | |
US204617P | 2000-05-16 | ||
PCT/US2001/016088 WO2001087925A2 (en) | 2000-05-16 | 2001-05-16 | Methods for refolding proteins containing free cysteine residues |
Publications (2)
Publication Number | Publication Date |
---|---|
DE60129432D1 true DE60129432D1 (de) | 2007-08-30 |
DE60129432T2 DE60129432T2 (de) | 2008-04-17 |
Family
ID=22758675
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE60129432T Expired - Lifetime DE60129432T2 (de) | 2000-05-16 | 2001-05-16 | Verfahren zur rückfaltung von proteinen mit freien cysteinresten |
Country Status (13)
Country | Link |
---|---|
US (3) | US7306931B2 (de) |
EP (1) | EP1284987B1 (de) |
JP (2) | JP4873818B2 (de) |
CN (1) | CN1318443C (de) |
AT (1) | ATE367398T1 (de) |
AU (2) | AU2001274853B2 (de) |
CA (1) | CA2408851C (de) |
DE (1) | DE60129432T2 (de) |
ES (1) | ES2290142T3 (de) |
HK (1) | HK1050014A1 (de) |
IL (2) | IL152804A0 (de) |
NZ (1) | NZ522847A (de) |
WO (1) | WO2001087925A2 (de) |
Families Citing this family (117)
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US20080076706A1 (en) * | 1997-07-14 | 2008-03-27 | Bolder Biotechnology, Inc. | Derivatives of Growth Hormone and Related Proteins, and Methods of Use Thereof |
US7270809B2 (en) * | 1997-07-14 | 2007-09-18 | Bolder Biotechnology, Inc. | Cysteine variants of alpha interferon-2 |
US6753165B1 (en) * | 1999-01-14 | 2004-06-22 | Bolder Biotechnology, Inc. | Methods for making proteins containing free cysteine residues |
US7495087B2 (en) | 1997-07-14 | 2009-02-24 | Bolder Biotechnology, Inc. | Cysteine muteins in the C-D loop of human interleukin-11 |
US8288126B2 (en) | 1999-01-14 | 2012-10-16 | Bolder Biotechnology, Inc. | Methods for making proteins containing free cysteine residues |
US6677433B2 (en) * | 1999-01-27 | 2004-01-13 | The Regents Of The University Of California | Stabilization of hypoallergenic, hyperdigestible previously reduced proteins |
KR100356140B1 (ko) * | 1999-07-08 | 2002-10-19 | 한미약품공업 주식회사 | 인간 과립구 콜로니 자극인자 변이체 및 이의 생산 방법 |
NZ522847A (en) | 2000-05-16 | 2004-11-26 | Bolder Biotechnology Inc | Methods for refolding proteins containing free cysteine residues |
US7173003B2 (en) | 2001-10-10 | 2007-02-06 | Neose Technologies, Inc. | Granulocyte colony stimulating factor: remodeling and glycoconjugation of G-CSF |
US7214660B2 (en) | 2001-10-10 | 2007-05-08 | Neose Technologies, Inc. | Erythropoietin: remodeling and glycoconjugation of erythropoietin |
EP1314739A1 (de) | 2001-11-22 | 2003-05-28 | Bayer Ag | Verfahren zur Renaturierung rekombinanter, Disulfid-enthaltender Proteine bei hohen Proteinkonzentrationen in Gegenwart von Aminen |
DE60335608D1 (de) | 2002-02-27 | 2011-02-17 | Pharmain Corp | Zusammensetzungen zur abgabe von therapeutika und anderen materialien und verfahren zu ihrer herstellung und verwendung |
US7635463B2 (en) | 2002-02-27 | 2009-12-22 | Pharmain Corporation | Compositions for delivery of therapeutics and other materials |
WO2003078461A1 (en) | 2002-03-20 | 2003-09-25 | Biopolymed Inc. | Preparation of g-csf stoichiometrically conjugated with biocompatible polymers at cystein residue |
CA2498319A1 (en) * | 2002-09-09 | 2004-03-18 | Nautilus Biotech | Rational evolution of cytokines for higher stability, the cytokines and encoding nucleic acid molecules |
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US7314613B2 (en) | 2002-11-18 | 2008-01-01 | Maxygen, Inc. | Interferon-alpha polypeptides and conjugates |
PL219741B1 (pl) | 2002-12-26 | 2015-07-31 | Mountain View Pharmaceuticals | Sposób zwiększania antyproliferacyjnego potencjału nieglikozylowanego interferonu-beta-1b in vitro, koniugat wytworzony tym sposobem i jego zastosowanie oraz kompozycja farmaceutyczna zawierająca koniugat i jej zastosowanie |
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SI1596887T1 (sl) | 2003-02-26 | 2022-05-31 | Nektar Therapeutics | Konjugati polimer-del faktorja VIII |
US20060104968A1 (en) | 2003-03-05 | 2006-05-18 | Halozyme, Inc. | Soluble glycosaminoglycanases and methods of preparing and using soluble glycosaminogly ycanases |
AU2004227937B2 (en) | 2003-03-31 | 2007-09-20 | Xencor, Inc | Methods for rational pegylation of proteins |
US7642340B2 (en) | 2003-03-31 | 2010-01-05 | Xencor, Inc. | PEGylated TNF-α variant proteins |
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2001
- 2001-05-16 NZ NZ522847A patent/NZ522847A/en not_active IP Right Cessation
- 2001-05-16 CN CNB018128556A patent/CN1318443C/zh not_active Expired - Fee Related
- 2001-05-16 JP JP2001585144A patent/JP4873818B2/ja not_active Expired - Fee Related
- 2001-05-16 IL IL15280401A patent/IL152804A0/xx unknown
- 2001-05-16 CA CA2408851A patent/CA2408851C/en not_active Expired - Lifetime
- 2001-05-16 AU AU2001274853A patent/AU2001274853B2/en not_active Ceased
- 2001-05-16 AU AU7485301A patent/AU7485301A/xx active Pending
- 2001-05-16 ES ES01941504T patent/ES2290142T3/es not_active Expired - Lifetime
- 2001-05-16 US US10/276,358 patent/US7306931B2/en not_active Expired - Lifetime
- 2001-05-16 DE DE60129432T patent/DE60129432T2/de not_active Expired - Lifetime
- 2001-05-16 AT AT01941504T patent/ATE367398T1/de not_active IP Right Cessation
- 2001-05-16 EP EP01941504A patent/EP1284987B1/de not_active Expired - Lifetime
- 2001-05-16 WO PCT/US2001/016088 patent/WO2001087925A2/en active IP Right Grant
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- 2007-12-11 US US12/001,639 patent/US20090269804A1/en not_active Abandoned
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CN1318443C (zh) | 2007-05-30 |
US20040018586A1 (en) | 2004-01-29 |
CA2408851A1 (en) | 2001-11-22 |
EP1284987B1 (de) | 2007-07-18 |
EP1284987A2 (de) | 2003-02-26 |
JP5517991B2 (ja) | 2014-06-11 |
IL152804A0 (en) | 2003-06-24 |
JP4873818B2 (ja) | 2012-02-08 |
AU7485301A (en) | 2001-11-26 |
CN1443194A (zh) | 2003-09-17 |
HK1050014A1 (en) | 2003-06-06 |
NZ522847A (en) | 2004-11-26 |
US20110189124A1 (en) | 2011-08-04 |
US8932828B2 (en) | 2015-01-13 |
DE60129432T2 (de) | 2008-04-17 |
JP2011173911A (ja) | 2011-09-08 |
IL152804A (en) | 2010-11-30 |
AU2001274853C1 (en) | 2001-11-26 |
WO2001087925A3 (en) | 2002-08-01 |
WO2001087925A2 (en) | 2001-11-22 |
ATE367398T1 (de) | 2007-08-15 |
US7306931B2 (en) | 2007-12-11 |
US20090269804A1 (en) | 2009-10-29 |
ES2290142T3 (es) | 2008-02-16 |
CA2408851C (en) | 2011-07-12 |
AU2001274853B2 (en) | 2007-03-22 |
JP2004508014A (ja) | 2004-03-18 |
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