DE60234811D1 - Verfahren und zusammensetzungen zur extraktion von proteinen aus zellen - Google Patents
Verfahren und zusammensetzungen zur extraktion von proteinen aus zellenInfo
- Publication number
- DE60234811D1 DE60234811D1 DE60234811T DE60234811T DE60234811D1 DE 60234811 D1 DE60234811 D1 DE 60234811D1 DE 60234811 T DE60234811 T DE 60234811T DE 60234811 T DE60234811 T DE 60234811T DE 60234811 D1 DE60234811 D1 DE 60234811D1
- Authority
- DE
- Germany
- Prior art keywords
- cells
- proteins
- extraction
- compositions
- detergent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title abstract 3
- 102000004169 proteins and genes Human genes 0.000 title abstract 3
- 108090000623 proteins and genes Proteins 0.000 title abstract 3
- 238000000605 extraction Methods 0.000 title 1
- 239000000203 mixture Substances 0.000 title 1
- 241000700605 Viruses Species 0.000 abstract 1
- 239000003599 detergent Substances 0.000 abstract 1
- 230000000415 inactivating effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/804—Single cell protein
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/766,043 US7229789B1 (en) | 2001-01-19 | 2001-01-19 | Methods and compositions for extracting proteins from cells |
| PCT/US2002/001670 WO2002057478A1 (en) | 2001-01-19 | 2002-01-18 | Methods and compositions for extracting proteins from cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE60234811D1 true DE60234811D1 (de) | 2010-02-04 |
Family
ID=25075221
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60234811T Expired - Lifetime DE60234811D1 (de) | 2001-01-19 | 2002-01-18 | Verfahren und zusammensetzungen zur extraktion von proteinen aus zellen |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US7229789B1 (de) |
| EP (1) | EP1356076B1 (de) |
| JP (1) | JP4372421B2 (de) |
| AT (1) | ATE452984T1 (de) |
| CA (1) | CA2434844A1 (de) |
| DE (1) | DE60234811D1 (de) |
| ES (1) | ES2336548T3 (de) |
| WO (1) | WO2002057478A1 (de) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7229789B1 (en) * | 2001-01-19 | 2007-06-12 | N.V. Organon | Methods and compositions for extracting proteins from cells |
| US20040048315A1 (en) | 2002-08-28 | 2004-03-11 | Pharmacia Corporation | Method for the preparation of growth hormone and antagonist thereof having lower levels of isoform impurities thereof |
| CN1703421B (zh) | 2002-09-20 | 2010-06-23 | 法玛西亚公司 | 降低peg化蛋白的聚集体水平的方法 |
| CA2504129A1 (en) | 2002-11-01 | 2004-05-21 | Promega Corporation | Cell lysis compositions, methods of use, apparatus, and kit |
| DE10309691B4 (de) * | 2003-02-28 | 2018-10-04 | Hans-Knöll-Institut für Naturstoff-Forschung e.V. | Gewinnung von löslichen Proteinen aus der Biomasse rekombinanter Mikroorganismen |
| US7935798B2 (en) * | 2004-02-27 | 2011-05-03 | Dow Global Technologies Llc | Method for the extraction of intracellular proteins from a fermentation broth |
| WO2006074178A2 (en) * | 2005-01-04 | 2006-07-13 | Invitrogen Corporation | Alkyl phosphine detergent products and extraction processes |
| DE102005015005A1 (de) * | 2005-04-01 | 2006-10-05 | Qiagen Gmbh | Verfahren zur Behandlung einer Biomoleküle enthaltenden Probe |
| AU2008335778B2 (en) | 2007-12-11 | 2014-04-10 | The Scripps Research Institute | In vivo unnatural amino acid expression in the methylotrophic yeast Pichia pastoris |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5011915A (en) | 1987-10-26 | 1991-04-30 | Merck & Co., Inc. | Process for purifying recombinant hepatitis antigens |
| US5407810A (en) | 1993-08-20 | 1995-04-18 | Genentech, Inc. | Aqueous multiple-phase isolation of polypeptide |
| US7229789B1 (en) * | 2001-01-19 | 2007-06-12 | N.V. Organon | Methods and compositions for extracting proteins from cells |
-
2001
- 2001-01-19 US US09/766,043 patent/US7229789B1/en active Active
-
2002
- 2002-01-18 JP JP2002558530A patent/JP4372421B2/ja not_active Expired - Lifetime
- 2002-01-18 DE DE60234811T patent/DE60234811D1/de not_active Expired - Lifetime
- 2002-01-18 WO PCT/US2002/001670 patent/WO2002057478A1/en active Application Filing
- 2002-01-18 AT AT02707529T patent/ATE452984T1/de not_active IP Right Cessation
- 2002-01-18 EP EP02707529A patent/EP1356076B1/de not_active Expired - Lifetime
- 2002-01-18 CA CA002434844A patent/CA2434844A1/en not_active Abandoned
- 2002-01-18 US US10/052,690 patent/US6821752B2/en not_active Expired - Lifetime
- 2002-01-18 ES ES02707529T patent/ES2336548T3/es not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP1356076B1 (de) | 2009-12-23 |
| EP1356076A4 (de) | 2004-03-17 |
| US20030003534A1 (en) | 2003-01-02 |
| CA2434844A1 (en) | 2002-07-25 |
| EP1356076A1 (de) | 2003-10-29 |
| US7229789B1 (en) | 2007-06-12 |
| JP4372421B2 (ja) | 2009-11-25 |
| US6821752B2 (en) | 2004-11-23 |
| ES2336548T3 (es) | 2010-04-14 |
| ATE452984T1 (de) | 2010-01-15 |
| WO2002057478A1 (en) | 2002-07-25 |
| JP2004520040A (ja) | 2004-07-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE10290025T1 (de) | Verfahren zur Differenzierung von Stammzellen in Insulin-produzierende Zellen | |
| DE60328383D1 (de) | Zusammensetzungen zur zuführung von nukleinsäuren an zellen | |
| DE60326012D1 (de) | Verfahren zur Konzentration von Proteinen | |
| DE60207148D1 (de) | Vorrichtung und Verfahren zur Gewinnung von Zellen | |
| DE69721893D1 (de) | Verfahren zur Abscheidung von Zellen aus Blut | |
| DE69940321D1 (de) | Zusammensetzung und verfahren zur identifizierung von retikulierten zellen | |
| DE69939945D1 (de) | Verfahren zur isolierung von stammzellen | |
| BR0213786A (pt) | Peptìdeos efetivos no tratamento de tumores e outras condições que requerem a remoção ou destruição de células | |
| DE60238493D1 (de) | Is von seltenen zellen verwendete markierte zellen | |
| DE60234425D1 (de) | Verfahren zur optimaliserung von verkerhrsinformationsinhalt | |
| DE60117686D1 (de) | Verfahren und vorrichtung zur abtrennung von ionenarten aus einer flüssigkeit | |
| DE60135498D1 (de) | Produktion von ganzen Antikörpern in prokaryontischen Zellen | |
| DE69908795D1 (de) | Verfahren zur isolierung von nukleinsäure und vorrichtung hierfür | |
| BR0211199A (pt) | Peptìdeos efetivos no tratamento de tumores e outras condições que requerem a remoção ou destruição de células | |
| DE60139716D1 (de) | Vorrichtung zur vorbereitung von zellen | |
| ATE366801T1 (de) | Zusammensetzungen und verfahren zur trennung von zellen | |
| DE60234811D1 (de) | Verfahren und zusammensetzungen zur extraktion von proteinen aus zellen | |
| ATE466080T1 (de) | Formulierungen und verfahren zum denaturieren von proteinen | |
| DE502006002073D1 (de) | Verfahren zur verhinderung der zeitabhängigen rna-expression in biologischen zellen | |
| ATE410438T1 (de) | Verfahren zum entfernen von viren in fibrinogenlösungen | |
| DE60323579D1 (de) | Methode zur Separierung von Nukleinsäuren und dafür verwendbare Reagenz | |
| ATE384263T1 (de) | Verfahren zur bestimmung von biologischen agenzien in lebenden zielzellen | |
| DE602004023272D1 (de) | Verfahren zur erkennung, analyse und isolation von entstehenden proteinen | |
| DE50204289D1 (de) | Vorrichtung und verfahren zur selektion von selbstbeweglichen biologischen spezies, insbesondere von samenzellen | |
| DE50106557D1 (de) | Verfahren zur aufarbeitung von emulsionspolymerisaten |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition | ||
| R082 | Change of representative |
Ref document number: 1356076 Country of ref document: EP Representative=s name: FDST PATENTANWAELTE FREIER DOERR STAMMLER TSCH, DE |
|
| R081 | Change of applicant/patentee |
Ref document number: 1356076 Country of ref document: EP Owner name: FUJIFILM DIOSYNTH BIOTECHNOLOGIES U.S.A., INC., US Free format text: FORMER OWNER: N.V. ORGANON, 5349 OSS, NL Effective date: 20120525 |
|
| R082 | Change of representative |
Ref document number: 1356076 Country of ref document: EP Representative=s name: FDST PATENTANWAELTE FREIER DOERR STAMMLER TSCH, DE Effective date: 20120703 Ref document number: 1356076 Country of ref document: EP Representative=s name: FDST PATENTANWAELTE FREIER DOERR STAMMLER TSCH, DE Effective date: 20120525 Ref document number: 1356076 Country of ref document: EP Representative=s name: , Effective date: 20120703 Ref document number: 1356076 Country of ref document: EP Representative=s name: , Effective date: 20120710 Ref document number: 1356076 Country of ref document: EP Representative=s name: , Effective date: 20120525 |