DE60042366D1 - Methoden zur reinigung von dns - Google Patents

Methoden zur reinigung von dns

Info

Publication number
DE60042366D1
DE60042366D1 DE60042366T DE60042366T DE60042366D1 DE 60042366 D1 DE60042366 D1 DE 60042366D1 DE 60042366 T DE60042366 T DE 60042366T DE 60042366 T DE60042366 T DE 60042366T DE 60042366 D1 DE60042366 D1 DE 60042366D1
Authority
DE
Germany
Prior art keywords
plasmid dna
methods
dna
purification
aqueous solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60042366T
Other languages
English (en)
Inventor
Natarajan Ramasubramanyan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lonza Biologics Inc
Original Assignee
Lonza Biologics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22474295&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE60042366(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Lonza Biologics Inc filed Critical Lonza Biologics Inc
Application granted granted Critical
Publication of DE60042366D1 publication Critical patent/DE60042366D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Saccharide Compounds (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Peptides Or Proteins (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE60042366T 1999-05-28 2000-05-26 Methoden zur reinigung von dns Expired - Lifetime DE60042366D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13677299P 1999-05-28 1999-05-28
PCT/US2000/014527 WO2000073318A1 (en) 1999-05-28 2000-05-26 Methods of dna purification and purified dna

Publications (1)

Publication Number Publication Date
DE60042366D1 true DE60042366D1 (de) 2009-07-23

Family

ID=22474295

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60042366T Expired - Lifetime DE60042366D1 (de) 1999-05-28 2000-05-26 Methoden zur reinigung von dns

Country Status (21)

Country Link
EP (1) EP1187840B2 (de)
JP (2) JP5320528B2 (de)
KR (1) KR100688349B1 (de)
CN (1) CN1264854C (de)
AT (1) ATE433456T1 (de)
AU (1) AU782153B2 (de)
BR (1) BR0011033A (de)
CA (1) CA2375215C (de)
CZ (1) CZ20014274A3 (de)
DE (1) DE60042366D1 (de)
DK (1) DK1187840T4 (de)
ES (1) ES2328008T5 (de)
HU (1) HUP0301869A2 (de)
IL (3) IL146695A0 (de)
MX (1) MXPA01012213A (de)
NO (1) NO20015703L (de)
NZ (1) NZ515645A (de)
PL (1) PL203013B1 (de)
RO (1) RO122855B1 (de)
RU (1) RU2001135838A (de)
WO (1) WO2000073318A1 (de)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1264854C (zh) * 1999-05-28 2006-07-19 坎布雷科斯生物科学巴尔的摩公司 Dna纯化方法和纯化的dna
GB0107634D0 (en) * 2001-03-27 2001-05-16 Fermentas Ab Nucleic acid purification
GB0127803D0 (en) * 2001-11-20 2002-01-09 Glaxo Group Ltd Processing nucleic acid
KR100463415B1 (ko) * 2001-12-12 2004-12-23 씨제이 주식회사 세균내ｄｎａ 또는 ｒｎａ로부터 리포폴리사카라이드를제거하는 방법
SE0200543D0 (sv) 2002-02-21 2002-02-21 Amersham Biosciences Ab Method of separation using aromatic thioether ligands
US20040127648A1 (en) * 2002-12-31 2004-07-01 Ciphergen Biosystems, Inc. Sorbent and method for the separation of plasmid DNA
EA009447B1 (ru) * 2003-09-17 2007-12-28 Сентельон Способ получения плазмидной днк фармацевтического качества
MXPA06011568A (es) * 2004-04-19 2006-12-20 Centelion Metodo para purificar adn plasmidico.
KR100612869B1 (ko) * 2004-11-08 2006-08-14 삼성전자주식회사 고정화된 금속-리간드 복합체에 의한 세포 용해 방법
JP4811773B2 (ja) * 2005-06-08 2011-11-09 肇 柄谷 核酸の精製法
US20090047734A1 (en) * 2006-03-31 2009-02-19 Ge Healthcare Bio-Sciences Ab Method of separation of deoxyribonucleic acids
US20120220729A1 (en) 2011-02-28 2012-08-30 Boehringer Ingelheim International Gmbh Liquid phase separation of plasmid dna isoforms and topoisomers
WO2015050191A1 (ja) 2013-10-03 2015-04-09 協和発酵バイオ株式会社 二重鎖リボ核酸の精製方法
CN109641928B (zh) * 2016-06-14 2022-08-30 比奥根Ma公司 用于寡核苷酸纯化的疏水性相互作用色谱法
EP3765023A4 (de) * 2018-03-15 2022-01-19 Intergalactic Therapeutics, Inc. Synthetische dns-vektoren und verwendungsverfahren
US11865480B2 (en) 2019-05-07 2024-01-09 Electronics and Telecommunications Research Institute Cancer Rop Co., Ltd. Nucleic acid purification device and nucleic acid purification method
EP4031168A4 (de) 2019-09-18 2023-10-11 Intergalactic Therapeutics, Inc. Synthetische dns-vektoren und verwendungsverfahren
CN111721871A (zh) * 2020-06-24 2020-09-29 南京济群生物科技有限公司 一种质粒超螺旋dna含量的高分离度检测方法
KR20220018187A (ko) 2020-08-06 2022-02-15 주식회사 바이오솔루션 플라스미드 dna 추출키트
KR20220018185A (ko) 2020-08-06 2022-02-15 주식회사 바이오솔루션 플라스미드 dna 추출방법
WO2022073995A1 (en) * 2020-10-05 2022-04-14 Bia Separations D.O.O. Improved removal of rna and contaminants from dna plasmid preparations by hydrophobic interaction chromatography

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5622960A (en) * 1992-04-14 1997-04-22 The United States Of America As Represented By The Department Of Health And Human Services Topoisomerase II inhibitors and therapeutic uses therefor
US5561064A (en) 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA
DE59505786D1 (de) 1994-02-07 1999-06-02 Qiagen Gmbh Verfahren zur abreicherung oder entfernung von endotoxinen
US5707850A (en) 1995-11-09 1998-01-13 Cole; Kenneth D. Concentration and size-fractionation of nucleic acids and viruses in porous media
US5707812A (en) 1996-08-06 1998-01-13 Vical Incorporated Purification of plasmid DNA during column chromatography
US6214586B1 (en) * 1997-12-08 2001-04-10 Genzyme Corporation Method for purifying plasmid DNA and plasmid DNA substantially free of genomic DNA
JP2000035423A (ja) * 1998-05-11 2000-02-02 Tosoh Corp 液体クロマトグラフィ―による核酸の分離法
EP0964057B1 (de) * 1998-05-11 2004-12-22 Tosoh Corporation Methode zur Trennung von Nucleinsäuren mittels Flüssigchromatographie
CN1264854C (zh) * 1999-05-28 2006-07-19 坎布雷科斯生物科学巴尔的摩公司 Dna纯化方法和纯化的dna

Also Published As

Publication number Publication date
WO2000073318A1 (en) 2000-12-07
NO20015703L (no) 2002-01-03
CA2375215C (en) 2012-11-20
PL364051A1 (en) 2004-12-13
RU2001135838A (ru) 2004-01-20
MXPA01012213A (es) 2003-06-24
BR0011033A (pt) 2003-07-01
IL146695A (en) 2012-12-31
HUP0301869A2 (hu) 2003-08-28
CZ20014274A3 (cs) 2003-01-15
IL222755A (en) 2015-08-31
RO122855B1 (ro) 2010-03-30
AU5165500A (en) 2000-12-18
JP2003524405A (ja) 2003-08-19
EP1187840B2 (de) 2012-12-19
KR100688349B1 (ko) 2007-02-28
CN1264854C (zh) 2006-07-19
ES2328008T5 (es) 2013-02-25
ATE433456T1 (de) 2009-06-15
DK1187840T3 (da) 2009-10-19
AU782153B2 (en) 2005-07-07
PL203013B1 (pl) 2009-08-31
IL222755A0 (en) 2012-12-31
JP5320528B2 (ja) 2013-10-23
DK1187840T4 (da) 2013-02-04
NO20015703D0 (no) 2001-11-22
IL146695A0 (en) 2002-07-25
CA2375215A1 (en) 2000-12-07
EP1187840B1 (de) 2009-06-10
KR20020092778A (ko) 2002-12-12
EP1187840A1 (de) 2002-03-20
NZ515645A (en) 2004-07-30
CN1390229A (zh) 2003-01-08
JP2012050463A (ja) 2012-03-15
ES2328008T3 (es) 2009-11-06
EP1187840A4 (de) 2002-09-18

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