HUP0301869A2 - DNS-tisztítási eljárások és a tisztított DNS - Google Patents

DNS-tisztítási eljárások és a tisztított DNS

Info

Publication number
HUP0301869A2
HUP0301869A2 HU0301869A HUP0301869A HUP0301869A2 HU P0301869 A2 HUP0301869 A2 HU P0301869A2 HU 0301869 A HU0301869 A HU 0301869A HU P0301869 A HUP0301869 A HU P0301869A HU P0301869 A2 HUP0301869 A2 HU P0301869A2
Authority
HU
Hungary
Prior art keywords
dna
methods
plasmid
purification
purified
Prior art date
Application number
HU0301869A
Other languages
English (en)
Inventor
Natarajan Ramasubramanyan
Original Assignee
Cambrex Bio Science, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22474295&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=HUP0301869(A2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Cambrex Bio Science, Inc. filed Critical Cambrex Bio Science, Inc.
Publication of HUP0301869A2 publication Critical patent/HUP0301869A2/hu

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Saccharide Compounds (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Peptides Or Proteins (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A találmány plazmidot tartalmazó vizes oldatból plazmid DNS tisztításieljárásokat biztosít, beleértve a gazdasejt szennyezők, mint példáulaz endotoxinok, RNS-ek, fehérjék és a gazdasejt DNS eltávolítását,valamint olyan eljárásokról gondoskodik, melyek hidrofób közegsegítségével a szupertekeredett, töréses vagy relaxált plazmid DNS-keveréket tartalmazó vizes oldatból a szupertekeredett plazmid DNSelkülönítését és tisztítását biztosítják. Ó
HU0301869A 1999-05-28 2000-05-26 DNS-tisztítási eljárások és a tisztított DNS HUP0301869A2 (hu)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13677299P 1999-05-28 1999-05-28
PCT/US2000/014527 WO2000073318A1 (en) 1999-05-28 2000-05-26 Methods of dna purification and purified dna

Publications (1)

Publication Number Publication Date
HUP0301869A2 true HUP0301869A2 (hu) 2003-08-28

Family

ID=22474295

Family Applications (1)

Application Number Title Priority Date Filing Date
HU0301869A HUP0301869A2 (hu) 1999-05-28 2000-05-26 DNS-tisztítási eljárások és a tisztított DNS

Country Status (21)

Country Link
EP (1) EP1187840B2 (hu)
JP (2) JP5320528B2 (hu)
KR (1) KR100688349B1 (hu)
CN (1) CN1264854C (hu)
AT (1) ATE433456T1 (hu)
AU (1) AU782153B2 (hu)
BR (1) BR0011033A (hu)
CA (1) CA2375215C (hu)
CZ (1) CZ20014274A3 (hu)
DE (1) DE60042366D1 (hu)
DK (1) DK1187840T4 (hu)
ES (1) ES2328008T5 (hu)
HU (1) HUP0301869A2 (hu)
IL (3) IL146695A0 (hu)
MX (1) MXPA01012213A (hu)
NO (1) NO20015703L (hu)
NZ (1) NZ515645A (hu)
PL (1) PL203013B1 (hu)
RO (1) RO122855B1 (hu)
RU (1) RU2001135838A (hu)
WO (1) WO2000073318A1 (hu)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5320528B2 (ja) * 1999-05-28 2013-10-23 ロンザ・バイオロジックス・インク Ｄｎａ精製方法および精製されたｄｎａ
GB0107634D0 (en) * 2001-03-27 2001-05-16 Fermentas Ab Nucleic acid purification
GB0127803D0 (en) * 2001-11-20 2002-01-09 Glaxo Group Ltd Processing nucleic acid
KR100463415B1 (ko) * 2001-12-12 2004-12-23 씨제이 주식회사 세균내ｄｎａ 또는 ｒｎａ로부터 리포폴리사카라이드를제거하는 방법
SE0200543D0 (sv) * 2002-02-21 2002-02-21 Amersham Biosciences Ab Method of separation using aromatic thioether ligands
US20040127648A1 (en) * 2002-12-31 2004-07-01 Ciphergen Biosystems, Inc. Sorbent and method for the separation of plasmid DNA
ES2376173T3 (es) * 2003-09-17 2012-03-09 Aventis Pharma S.A. Método de preparación de adn plasm�?dico de grado farmacéutico.
KR20060135062A (ko) * 2004-04-19 2006-12-28 센텔리옹 에스아에스 플라스미드 ｄｎａ의 정제 방법
KR100612869B1 (ko) * 2004-11-08 2006-08-14 삼성전자주식회사 고정화된 금속-리간드 복합체에 의한 세포 용해 방법
JP4811773B2 (ja) * 2005-06-08 2011-11-09 肇 柄谷 核酸の精製法
US20090047734A1 (en) * 2006-03-31 2009-02-19 Ge Healthcare Bio-Sciences Ab Method of separation of deoxyribonucleic acids
US20120220729A1 (en) 2011-02-28 2012-08-30 Boehringer Ingelheim International Gmbh Liquid phase separation of plasmid dna isoforms and topoisomers
US20160215010A1 (en) 2013-10-03 2016-07-28 Kyowa Hakko Bio Co., Ltd. Method for purifying double-stranded ribonucleic acid
WO2017218454A1 (en) * 2016-06-14 2017-12-21 Biogen Ma Inc. Hydrophobic interaction chromatography for purification of oligonucleotides
WO2019178500A1 (en) * 2018-03-15 2019-09-19 Limelight Bio, Inc. Synthetic dna vectors and methods of use
US11865480B2 (en) 2019-05-07 2024-01-09 Electronics and Telecommunications Research Institute Cancer Rop Co., Ltd. Nucleic acid purification device and nucleic acid purification method
AU2020351204A1 (en) 2019-09-18 2022-04-21 Intergalactic Therapeutics, Inc. Synthetic DNA vectors and methods of use
CN111721871A (zh) * 2020-06-24 2020-09-29 南京济群生物科技有限公司 一种质粒超螺旋dna含量的高分离度检测方法
KR20220018185A (ko) 2020-08-06 2022-02-15 주식회사 바이오솔루션 플라스미드 dna 추출방법
KR20220018187A (ko) 2020-08-06 2022-02-15 주식회사 바이오솔루션 플라스미드 dna 추출키트
JP2023545998A (ja) * 2020-10-05 2023-11-01 ザルトリウス ビーアイエー セパレーションズ ディー．オー．オー． 疎水性相互作用クロマトグラフィーによるｄｎａプラスミド調製物からのｒｎａ及び不純物の改善された除去

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5622960A (en) * 1992-04-14 1997-04-22 The United States Of America As Represented By The Department Of Health And Human Services Topoisomerase II inhibitors and therapeutic uses therefor
US5561064A (en) 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA
DE59507433D1 (de) 1994-02-07 2000-01-20 Qiagen Gmbh Verfahren zur herstellung endotoxinfreier oder an endotoxin abgereicherter nucleinsäuren und/oder oligonucleotide für die gentherapie
US5707850A (en) 1995-11-09 1998-01-13 Cole; Kenneth D. Concentration and size-fractionation of nucleic acids and viruses in porous media
US5707812A (en) 1996-08-06 1998-01-13 Vical Incorporated Purification of plasmid DNA during column chromatography
US6214586B1 (en) * 1997-12-08 2001-04-10 Genzyme Corporation Method for purifying plasmid DNA and plasmid DNA substantially free of genomic DNA
JP2000035423A (ja) * 1998-05-11 2000-02-02 Tosoh Corp 液体クロマトグラフィ―による核酸の分離法
US6441160B2 (en) * 1998-05-11 2002-08-27 Tosoh Corporation Separating plasmids from contaminants using hydrophobic or hydrophobic and ion exchange chromatography
JP5320528B2 (ja) * 1999-05-28 2013-10-23 ロンザ・バイオロジックス・インク Ｄｎａ精製方法および精製されたｄｎａ

Also Published As

Publication number Publication date
DE60042366D1 (de) 2009-07-23
CA2375215A1 (en) 2000-12-07
AU782153B2 (en) 2005-07-07
JP2012050463A (ja) 2012-03-15
DK1187840T4 (da) 2013-02-04
IL146695A (en) 2012-12-31
IL222755A0 (en) 2012-12-31
JP5320528B2 (ja) 2013-10-23
NO20015703L (no) 2002-01-03
RU2001135838A (ru) 2004-01-20
MXPA01012213A (es) 2003-06-24
PL203013B1 (pl) 2009-08-31
WO2000073318A1 (en) 2000-12-07
NO20015703D0 (no) 2001-11-22
KR100688349B1 (ko) 2007-02-28
EP1187840B2 (en) 2012-12-19
JP2003524405A (ja) 2003-08-19
RO122855B1 (ro) 2010-03-30
ATE433456T1 (de) 2009-06-15
NZ515645A (en) 2004-07-30
CN1390229A (zh) 2003-01-08
PL364051A1 (en) 2004-12-13
EP1187840A1 (en) 2002-03-20
ES2328008T3 (es) 2009-11-06
ES2328008T5 (es) 2013-02-25
IL146695A0 (en) 2002-07-25
CN1264854C (zh) 2006-07-19
AU5165500A (en) 2000-12-18
CA2375215C (en) 2012-11-20
IL222755A (en) 2015-08-31
CZ20014274A3 (cs) 2003-01-15
EP1187840A4 (en) 2002-09-18
DK1187840T3 (da) 2009-10-19
BR0011033A (pt) 2003-07-01
EP1187840B1 (en) 2009-06-10
KR20020092778A (ko) 2002-12-12

Similar Documents

Publication Publication Date Title
HUP0301869A2 (hu) DNS-tisztítási eljárások és a tisztított DNS
DE69732360D1 (de) Plasmide dna reinigung durch peg-fällung und säulen-chromatographie
DK0923592T3 (da) Fremgangsmåder til rensning af nukleinsyrer
IT8721618A0 (it) Prodecimento per la depurazione bilogica di acque reflue.
BR0211470A (pt) Sistema de reciclagem de gás, e, processo para a purificação e a reciclagem de um gás
DE60120151D1 (de) Isolierung von mesenchymalen stammzellen und deren verwendung
PT1200578E (pt) Metodo para purificacao de adn de plasmideo sem solventes organicos e sem arnase utilizando filtracao de fluxo tangencial
DE50015677D1 (de) Methode zur isolierung von dna aus biologischen materialien
DE60327471D1 (de) Verfahren und zusammensetzungen zur reinigung von nukleinsäure aus einer wirtszelle
CY1107777T1 (el) Μεθοδος για τον κλιμακουμενο καθαρισμο πλασμιδιακου dna
DE60114348D1 (de) Verfahren zur Reinigung von Gasen
PE20030298A1 (es) Filtros de agua y procesos para la utilizacion de los mismos
DE50208601D1 (de) Verfahren zur aufreinigung von bakterienzellen und zellbestandteilen
ATE420167T1 (de) Reinigungsverfahren für plasmid-dna
HK1042116A1 (zh) 淨化或隔離過濾病毒核酸的方法
WO2002044414A3 (en) Purification of dna sequencing reactions using silica magnetic particles
ZA200005663B (en) Method of water purification.
WO2000050644A3 (en) Methods for purifying dna using immobilized capture probes
MXPA02001009A (es) Virus de la influenza atenuado util como vacuna.
BR9807061A (pt) Purificação de dna em uma centrìfuga de fluxo contìnuo
IL129727A0 (en) 1-(3-Aminoindazol-5-YL)-3-phenylmethyl-cyclic ureas useful as HIV protease inhibitors
WO2002003061A3 (de) Verfahren zur identifizierung von die für behandlung von virusinfektionen geeigneten stoffen
WO2006034009A3 (en) Purification of biomolecules from contaminating intact nucleic acids
Lashchevskij et al. Use of the methods of acoustic coagulation of aerosols for purification of gaseous effluents from highly dispersed radioactive aerosols; Ispol'zovanie metoda akusticheskoj koagulyatsii aehrozolej dlya ochistki gazovykh vybrosov ot vysokodispersnykh radioaktivnykh aehrozolej
ES2162560A1 (es) Uso de fluoxetina, paroxetina y otros isrs para la fabricacion de medicamentos con el fin de aumentar la capacidad de abstenerse de substancias o actividades que crean dependencia.