DK1187840T4 - Fremgangsmåder til DNA-oprensning - Google Patents

Fremgangsmåder til DNA-oprensning

Info

Publication number
DK1187840T4
DK1187840T4 DK00936329.2T DK00936329T DK1187840T4 DK 1187840 T4 DK1187840 T4 DK 1187840T4 DK 00936329 T DK00936329 T DK 00936329T DK 1187840 T4 DK1187840 T4 DK 1187840T4
Authority
DK
Denmark
Prior art keywords
plasmid dna
methods
purification
aqueous solution
supercoiled
Prior art date
Application number
DK00936329.2T
Other languages
English (en)
Other versions
DK1187840T3 (da
Inventor
Natarajan Ramasubramanyan
Original Assignee
Lonza Biolog Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22474295&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK1187840(T4) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Lonza Biolog Inc filed Critical Lonza Biolog Inc
Publication of DK1187840T3 publication Critical patent/DK1187840T3/da
Application granted granted Critical
Publication of DK1187840T4 publication Critical patent/DK1187840T4/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Saccharide Compounds (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Peptides Or Proteins (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DK00936329.2T 1999-05-28 2000-05-26 Fremgangsmåder til DNA-oprensning DK1187840T4 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13677299P 1999-05-28 1999-05-28
PCT/US2000/014527 WO2000073318A1 (en) 1999-05-28 2000-05-26 Methods of dna purification and purified dna

Publications (2)

Publication Number Publication Date
DK1187840T3 DK1187840T3 (da) 2009-10-19
DK1187840T4 true DK1187840T4 (da) 2013-02-04

Family

ID=22474295

Family Applications (1)

Application Number Title Priority Date Filing Date
DK00936329.2T DK1187840T4 (da) 1999-05-28 2000-05-26 Fremgangsmåder til DNA-oprensning

Country Status (21)

Country Link
EP (1) EP1187840B2 (da)
JP (2) JP5320528B2 (da)
KR (1) KR100688349B1 (da)
CN (1) CN1264854C (da)
AT (1) ATE433456T1 (da)
AU (1) AU782153B2 (da)
BR (1) BR0011033A (da)
CA (1) CA2375215C (da)
CZ (1) CZ20014274A3 (da)
DE (1) DE60042366D1 (da)
DK (1) DK1187840T4 (da)
ES (1) ES2328008T5 (da)
HU (1) HUP0301869A2 (da)
IL (3) IL146695A0 (da)
MX (1) MXPA01012213A (da)
NO (1) NO20015703L (da)
NZ (1) NZ515645A (da)
PL (1) PL203013B1 (da)
RO (1) RO122855B1 (da)
RU (1) RU2001135838A (da)
WO (1) WO2000073318A1 (da)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL203013B1 (pl) * 1999-05-28 2009-08-31 Cambrex Bio Science Baltimore Sposób oczyszczania plazmidowego DNA, sposób oddzielania superskręconego plazmidowego DNA, sposób oddzielania endotoksyny od plazmidowego DNA, sposób wzbogacania ilości superskręconego DNA względem rozluźnionego DNA, sposób usuwania lipopolisacharydu
GB0107634D0 (en) * 2001-03-27 2001-05-16 Fermentas Ab Nucleic acid purification
GB0127803D0 (en) * 2001-11-20 2002-01-09 Glaxo Group Ltd Processing nucleic acid
KR100463415B1 (ko) * 2001-12-12 2004-12-23 씨제이 주식회사 세균내ｄｎａ 또는 ｒｎａ로부터 리포폴리사카라이드를제거하는 방법
SE0200543D0 (sv) 2002-02-21 2002-02-21 Amersham Biosciences Ab Method of separation using aromatic thioether ligands
US20040127648A1 (en) * 2002-12-31 2004-07-01 Ciphergen Biosystems, Inc. Sorbent and method for the separation of plasmid DNA
MXPA06003004A (es) * 2003-09-17 2006-06-23 Centelion Metodo de preparacion de acido desoxirribonucleico plasmidico de grado farmaceutico.
CA2559368A1 (en) * 2004-04-19 2005-10-27 Centelion Method for purifying plasmid dna
KR100612869B1 (ko) * 2004-11-08 2006-08-14 삼성전자주식회사 고정화된 금속-리간드 복합체에 의한 세포 용해 방법
JP4811773B2 (ja) * 2005-06-08 2011-11-09 肇 柄谷 核酸の精製法
US20090047734A1 (en) * 2006-03-31 2009-02-19 Ge Healthcare Bio-Sciences Ab Method of separation of deoxyribonucleic acids
US20120220729A1 (en) 2011-02-28 2012-08-30 Boehringer Ingelheim International Gmbh Liquid phase separation of plasmid dna isoforms and topoisomers
WO2015050191A1 (ja) 2013-10-03 2015-04-09 協和発酵バイオ株式会社 二重鎖リボ核酸の精製方法
CA3027288A1 (en) * 2016-06-14 2017-12-21 Biogen Ma Inc. Hydrophobic interaction chromatography for purification of oligonucleotides
KR20200132893A (ko) * 2018-03-15 2020-11-25 인터갈락틱 테라퓨틱스, 인크. 합성 dna 벡터 및 사용 방법
US11865480B2 (en) 2019-05-07 2024-01-09 Electronics and Telecommunications Research Institute Cancer Rop Co., Ltd. Nucleic acid purification device and nucleic acid purification method
GB2606844A (en) 2019-09-18 2022-11-23 Intergalactic Therapeutics Inc Synthetic DNA vectors and methods of use
CN111721871A (zh) * 2020-06-24 2020-09-29 南京济群生物科技有限公司 一种质粒超螺旋dna含量的高分离度检测方法
KR20220018185A (ko) 2020-08-06 2022-02-15 주식회사 바이오솔루션 플라스미드 dna 추출방법
KR20220018187A (ko) 2020-08-06 2022-02-15 주식회사 바이오솔루션 플라스미드 dna 추출키트
WO2022073995A1 (en) * 2020-10-05 2022-04-14 Bia Separations D.O.O. Improved removal of rna and contaminants from dna plasmid preparations by hydrophobic interaction chromatography

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5622960A (en) * 1992-04-14 1997-04-22 The United States Of America As Represented By The Department Of Health And Human Services Topoisomerase II inhibitors and therapeutic uses therefor
US5561064A (en) 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA
CA2182388C (en) 1994-02-07 2007-08-07 Metin Colpan Process for producing endotoxin-free or endotoxin-poor nucleic acids and/or oligonucleotides for gene therapy
US5707850A (en) 1995-11-09 1998-01-13 Cole; Kenneth D. Concentration and size-fractionation of nucleic acids and viruses in porous media
US5707812A (en) 1996-08-06 1998-01-13 Vical Incorporated Purification of plasmid DNA during column chromatography
US6214586B1 (en) * 1997-12-08 2001-04-10 Genzyme Corporation Method for purifying plasmid DNA and plasmid DNA substantially free of genomic DNA
EP0964057B1 (en) * 1998-05-11 2004-12-22 Tosoh Corporation Method for separating nucleic acids by means of liquid chromatography
JP2000035423A (ja) * 1998-05-11 2000-02-02 Tosoh Corp 液体クロマトグラフィ―による核酸の分離法
PL203013B1 (pl) * 1999-05-28 2009-08-31 Cambrex Bio Science Baltimore Sposób oczyszczania plazmidowego DNA, sposób oddzielania superskręconego plazmidowego DNA, sposób oddzielania endotoksyny od plazmidowego DNA, sposób wzbogacania ilości superskręconego DNA względem rozluźnionego DNA, sposób usuwania lipopolisacharydu

Also Published As

Publication number Publication date
JP2012050463A (ja) 2012-03-15
MXPA01012213A (es) 2003-06-24
DE60042366D1 (de) 2009-07-23
RU2001135838A (ru) 2004-01-20
CA2375215A1 (en) 2000-12-07
KR100688349B1 (ko) 2007-02-28
EP1187840A1 (en) 2002-03-20
JP2003524405A (ja) 2003-08-19
NZ515645A (en) 2004-07-30
KR20020092778A (ko) 2002-12-12
AU5165500A (en) 2000-12-18
HUP0301869A2 (hu) 2003-08-28
DK1187840T3 (da) 2009-10-19
RO122855B1 (ro) 2010-03-30
EP1187840B2 (en) 2012-12-19
AU782153B2 (en) 2005-07-07
ATE433456T1 (de) 2009-06-15
NO20015703L (no) 2002-01-03
CN1390229A (zh) 2003-01-08
EP1187840B1 (en) 2009-06-10
PL364051A1 (en) 2004-12-13
IL146695A0 (en) 2002-07-25
NO20015703D0 (no) 2001-11-22
CZ20014274A3 (cs) 2003-01-15
IL222755A0 (en) 2012-12-31
WO2000073318A1 (en) 2000-12-07
ES2328008T5 (es) 2013-02-25
PL203013B1 (pl) 2009-08-31
CN1264854C (zh) 2006-07-19
JP5320528B2 (ja) 2013-10-23
BR0011033A (pt) 2003-07-01
IL222755A (en) 2015-08-31
ES2328008T3 (es) 2009-11-06
CA2375215C (en) 2012-11-20
EP1187840A4 (en) 2002-09-18
IL146695A (en) 2012-12-31

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