BR0011033A - Métodos de purificação de dna e dna purificado - Google Patents

Métodos de purificação de dna e dna purificado

Info

Publication number
BR0011033A
BR0011033A BR0011033-7A BR0011033A BR0011033A BR 0011033 A BR0011033 A BR 0011033A BR 0011033 A BR0011033 A BR 0011033A BR 0011033 A BR0011033 A BR 0011033A
Authority
BR
Brazil
Prior art keywords
purified dna
dna
methods
plasmid dna
purification
Prior art date
Application number
BR0011033-7A
Other languages
English (en)
Inventor
Natarajan Ramasubramanyan
Original Assignee
Bio Science Contract Productio
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22474295&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BR0011033(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Bio Science Contract Productio filed Critical Bio Science Contract Productio
Publication of BR0011033A publication Critical patent/BR0011033A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Saccharide Compounds (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

"MéTODOS DE PURIFICAçãO DE DNA E DNA PURIFICADO". A presente invenção fornece métodos para a purificação de DNA de plasmídio que inclui remoção de impurezas da célula hospedeira, tais como endotoxinas, RNA, proteínas e DNA cromossómico, de uma solução aquosa contendo DNA de plasmídio e métodos para separação e purificação de DNA de plasmídio superespiralado de uma solução aquosa contendo uma mistura de DNA de plasmídio superespiralado e clivado ou relaxado usando suportes de cromatografia de interação hidrofóbicos.
BR0011033-7A 1999-05-28 2000-05-26 Métodos de purificação de dna e dna purificado BR0011033A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13677299P 1999-05-28 1999-05-28
PCT/US2000/014527 WO2000073318A1 (en) 1999-05-28 2000-05-26 Methods of dna purification and purified dna

Publications (1)

Publication Number Publication Date
BR0011033A true BR0011033A (pt) 2003-07-01

Family

ID=22474295

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0011033-7A BR0011033A (pt) 1999-05-28 2000-05-26 Métodos de purificação de dna e dna purificado

Country Status (21)

Country Link
EP (1) EP1187840B2 (pt)
JP (2) JP5320528B2 (pt)
KR (1) KR100688349B1 (pt)
CN (1) CN1264854C (pt)
AT (1) ATE433456T1 (pt)
AU (1) AU782153B2 (pt)
BR (1) BR0011033A (pt)
CA (1) CA2375215C (pt)
CZ (1) CZ20014274A3 (pt)
DE (1) DE60042366D1 (pt)
DK (1) DK1187840T4 (pt)
ES (1) ES2328008T5 (pt)
HU (1) HUP0301869A2 (pt)
IL (3) IL146695A0 (pt)
MX (1) MXPA01012213A (pt)
NO (1) NO20015703L (pt)
NZ (1) NZ515645A (pt)
PL (1) PL203013B1 (pt)
RO (1) RO122855B1 (pt)
RU (1) RU2001135838A (pt)
WO (1) WO2000073318A1 (pt)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL146695A0 (en) * 1999-05-28 2002-07-25 Bio Science Contract Productio Methods of dna purification and purified dna
GB0107634D0 (en) * 2001-03-27 2001-05-16 Fermentas Ab Nucleic acid purification
GB0127803D0 (en) * 2001-11-20 2002-01-09 Glaxo Group Ltd Processing nucleic acid
KR100463415B1 (ko) * 2001-12-12 2004-12-23 씨제이 주식회사 세균내ｄｎａ 또는 ｒｎａ로부터 리포폴리사카라이드를제거하는 방법
SE0200543D0 (sv) * 2002-02-21 2002-02-21 Amersham Biosciences Ab Method of separation using aromatic thioether ligands
US20040127648A1 (en) * 2002-12-31 2004-07-01 Ciphergen Biosystems, Inc. Sorbent and method for the separation of plasmid DNA
EA009447B1 (ru) * 2003-09-17 2007-12-28 Сентельон Способ получения плазмидной днк фармацевтического качества
CN101006170A (zh) * 2004-04-19 2007-07-25 森特利昂公司 纯化质粒dna的方法
KR100612869B1 (ko) * 2004-11-08 2006-08-14 삼성전자주식회사 고정화된 금속-리간드 복합체에 의한 세포 용해 방법
JP4811773B2 (ja) * 2005-06-08 2011-11-09 肇 柄谷 核酸の精製法
WO2007114762A1 (en) * 2006-03-31 2007-10-11 Ge Healthcare Bio-Sciences Ab Method of separation of deoxyribonucleic acids
US20120220729A1 (en) 2011-02-28 2012-08-30 Boehringer Ingelheim International Gmbh Liquid phase separation of plasmid dna isoforms and topoisomers
WO2015050191A1 (ja) 2013-10-03 2015-04-09 協和発酵バイオ株式会社 二重鎖リボ核酸の精製方法
JP7075359B2 (ja) * 2016-06-14 2022-05-25 バイオジェン・エムエイ・インコーポレイテッド オリゴヌクレオチドの精製のための疎水性相互作用クロマトグラフィー
WO2019178500A1 (en) * 2018-03-15 2019-09-19 Limelight Bio, Inc. Synthetic dna vectors and methods of use
US11865480B2 (en) 2019-05-07 2024-01-09 Electronics and Telecommunications Research Institute Cancer Rop Co., Ltd. Nucleic acid purification device and nucleic acid purification method
TW202124722A (zh) 2019-09-18 2021-07-01 美商英特佳樂帝克醫療公司 合成dna載體及其使用方法
CN111721871A (zh) * 2020-06-24 2020-09-29 南京济群生物科技有限公司 一种质粒超螺旋dna含量的高分离度检测方法
KR20220018187A (ko) 2020-08-06 2022-02-15 주식회사 바이오솔루션 플라스미드 dna 추출키트
KR20220018185A (ko) 2020-08-06 2022-02-15 주식회사 바이오솔루션 플라스미드 dna 추출방법
US20230374487A1 (en) * 2020-10-05 2023-11-23 Sartorius Bia Separations D.O.O. Improved removal of rna and contaminants from dna plasmid preparations by hydrophobic interaction chromatography

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5622960A (en) * 1992-04-14 1997-04-22 The United States Of America As Represented By The Department Of Health And Human Services Topoisomerase II inhibitors and therapeutic uses therefor
US5561064A (en) 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA
US5747663A (en) 1994-02-07 1998-05-05 Qiagen Gmbh Process for the depletion or removal of endotoxins
US5707850A (en) 1995-11-09 1998-01-13 Cole; Kenneth D. Concentration and size-fractionation of nucleic acids and viruses in porous media
US5707812A (en) 1996-08-06 1998-01-13 Vical Incorporated Purification of plasmid DNA during column chromatography
US6214586B1 (en) * 1997-12-08 2001-04-10 Genzyme Corporation Method for purifying plasmid DNA and plasmid DNA substantially free of genomic DNA
DE69922740T2 (de) * 1998-05-11 2005-12-08 Tosoh Corp., Shinnanyo Methode zur Trennung von Nucleinsäuren mittels Flüssigchromatographie
JP2000035423A (ja) * 1998-05-11 2000-02-02 Tosoh Corp 液体クロマトグラフィ―による核酸の分離法
IL146695A0 (en) * 1999-05-28 2002-07-25 Bio Science Contract Productio Methods of dna purification and purified dna

Also Published As

Publication number Publication date
IL146695A0 (en) 2002-07-25
EP1187840A4 (en) 2002-09-18
AU5165500A (en) 2000-12-18
JP2012050463A (ja) 2012-03-15
NZ515645A (en) 2004-07-30
DK1187840T3 (da) 2009-10-19
PL364051A1 (en) 2004-12-13
NO20015703L (no) 2002-01-03
EP1187840B1 (en) 2009-06-10
MXPA01012213A (es) 2003-06-24
ATE433456T1 (de) 2009-06-15
CZ20014274A3 (cs) 2003-01-15
CN1264854C (zh) 2006-07-19
DE60042366D1 (de) 2009-07-23
DK1187840T4 (da) 2013-02-04
KR20020092778A (ko) 2002-12-12
JP5320528B2 (ja) 2013-10-23
CA2375215C (en) 2012-11-20
IL146695A (en) 2012-12-31
CN1390229A (zh) 2003-01-08
IL222755A (en) 2015-08-31
JP2003524405A (ja) 2003-08-19
KR100688349B1 (ko) 2007-02-28
HUP0301869A2 (hu) 2003-08-28
RO122855B1 (ro) 2010-03-30
RU2001135838A (ru) 2004-01-20
PL203013B1 (pl) 2009-08-31
IL222755A0 (en) 2012-12-31
CA2375215A1 (en) 2000-12-07
EP1187840B2 (en) 2012-12-19
ES2328008T3 (es) 2009-11-06
ES2328008T5 (es) 2013-02-25
WO2000073318A1 (en) 2000-12-07
EP1187840A1 (en) 2002-03-20
AU782153B2 (en) 2005-07-07
NO20015703D0 (no) 2001-11-22

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Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 5A, 6A E 7A ANUIDADES.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 1977 DE 11/12/2007.