DE60042235D1 - Prozess zur trennung von proteinen - Google Patents

Prozess zur trennung von proteinen

Info

Publication number
DE60042235D1
DE60042235D1 DE60042235T DE60042235T DE60042235D1 DE 60042235 D1 DE60042235 D1 DE 60042235D1 DE 60042235 T DE60042235 T DE 60042235T DE 60042235 T DE60042235 T DE 60042235T DE 60042235 D1 DE60042235 D1 DE 60042235D1
Authority
DE
Germany
Prior art keywords
proteins
separating proteins
atps
protein
partitioning
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60042235T
Other languages
English (en)
Inventor
Merja Penttilae
Tiina Nakari-Setaelae
Richard Fagerstroem
Klaus Selber
Maria-Regina Kula
Markus Linder
Folke Tjerneld
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Valtion Teknillinen Tutkimuskeskus
Original Assignee
Valtion Teknillinen Tutkimuskeskus
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from FI990667A external-priority patent/FI990667A0/fi
Application filed by Valtion Teknillinen Tutkimuskeskus filed Critical Valtion Teknillinen Tutkimuskeskus
Application granted granted Critical
Publication of DE60042235D1 publication Critical patent/DE60042235D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
DE60042235T 1999-03-25 2000-03-24 Prozess zur trennung von proteinen Expired - Lifetime DE60042235D1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FI990667A FI990667A0 (fi) 1999-03-25 1999-03-25 Menetelmä molekyylien erottamiseksi
FI991782 1999-08-20
PCT/FI2000/000249 WO2000058342A1 (en) 1999-03-25 2000-03-24 Process for partitioning of proteins

Publications (1)

Publication Number Publication Date
DE60042235D1 true DE60042235D1 (de) 2009-07-02

Family

ID=26160722

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60042235T Expired - Lifetime DE60042235D1 (de) 1999-03-25 2000-03-24 Prozess zur trennung von proteinen

Country Status (14)

Country Link
US (2) US7060669B1 (de)
EP (1) EP1163260B1 (de)
JP (1) JP4570787B2 (de)
KR (1) KR100819187B1 (de)
CN (1) CN1249079C (de)
AT (1) ATE431834T1 (de)
AU (1) AU778477B2 (de)
CA (1) CA2367826A1 (de)
DE (1) DE60042235D1 (de)
DK (1) DK1163260T3 (de)
IL (2) IL145337A0 (de)
NO (1) NO20014534L (de)
NZ (1) NZ514891A (de)
WO (1) WO2000058342A1 (de)

Families Citing this family (45)

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FI120310B (fi) * 2001-02-13 2009-09-15 Valtion Teknillinen Parannettu menetelmä erittyvien proteiinien tuottamiseksi sienissä
FI116198B (fi) * 2001-07-16 2005-10-14 Valtion Teknillinen Polypeptidien immobilisointimenetelmä
ES2285631T3 (es) 2004-07-27 2007-11-16 Unilever N.V. Productos alimenticios aireados que contienen hidrofobinas.
US7241734B2 (en) 2004-08-18 2007-07-10 E. I. Du Pont De Nemours And Company Thermophilic hydrophobin proteins and applications for surface modification
US7147912B2 (en) 2004-08-18 2006-12-12 E. I. Du Pont De Nemours And Company Amphipathic proteinaceous coating on nanoporous polymer
US7892788B2 (en) 2005-02-07 2011-02-22 Basf Se Hydrophobin fusion products, production and use thereof
DE102005005737A1 (de) * 2005-02-07 2006-08-17 Basf Ag Neue Hydrophobinfusionsproteine, deren Herstellung und Verwendung
EP1866150B1 (de) 2005-03-31 2016-10-19 Basf Se Metallische substrate mit polypeptiden als haftvermittler
DK1869138T3 (da) 2005-04-01 2010-03-01 Basf Se Borevæske indeholdende hydrophobin
US8535535B2 (en) 2005-04-01 2013-09-17 Basf Se Use of hydrophobin as a phase stabilizer
US20100170142A1 (en) * 2005-04-01 2010-07-08 Basf Aktiengesellschaft Use of Proteins as Demulsifying Agents
DE102005027139A1 (de) 2005-06-10 2006-12-28 Basf Ag Neue Cystein-verarmte Hydrophobinfusionsproteine, deren Herstellung und Verwendung
ATE493898T1 (de) 2005-09-23 2011-01-15 Unilever Nv Durchlüftete produkte mit verringerter aufrahmung
JP5027138B2 (ja) 2005-09-23 2012-09-19 ユニリーバー・ナームローゼ・ベンノートシヤープ 低ｐＨ空気混入製品
DE102005048720A1 (de) 2005-10-12 2007-04-19 Basf Ag Verwendung von Proteinen als Antischaum-Komponente in Kraftstoffen
ES2306039T3 (es) 2005-12-21 2008-11-01 Unilever N.V. Dulce aireado congelado.
US8263343B2 (en) 2005-12-22 2012-09-11 Ge Healthcare Bio-Science Ab Preparation of biomolecules
ES2351479T3 (es) * 2006-01-31 2011-02-07 Unilever Plc Composiciones aireadas que comprenden hidrofobina.
US8096484B2 (en) 2006-08-15 2012-01-17 Basf Se Method for the production of dry free-flowing hydrophobin preparations
JP5245227B2 (ja) * 2006-08-23 2013-07-24 三菱瓦斯化学株式会社 疎水性化合物の起泡による分離法
CN100453642C (zh) * 2006-10-10 2009-01-21 山西职工医学院 一种过氧化物酶的提取方法
KR100836171B1 (ko) * 2007-01-19 2008-06-09 인하대학교 산학협력단 분배 향상 물질이 접합된 생물물질을 이용한 수성이상계추출법에서의 친화성 분리 방법
KR100859791B1 (ko) * 2007-05-23 2008-09-23 인하대학교 산학협력단 상 형성 물질과 결합된 분자인식 리간드를 이용하여 상형성과 분자특이적 분리를 동시에 수행하는 방법
ES2319844B1 (es) * 2007-09-12 2010-02-16 Universidad Miguel Hernandez De Elche (70%) Procedimiento de separacion de proteinas recombinantes en sistemas acuosos de dos fases.
CA2698293A1 (en) * 2007-09-13 2009-03-26 Thomas Subkowski Use of hydrophobin polypeptides as penetration intensifiers
NZ571979A (en) 2007-10-25 2010-05-28 Unilever Plc Aerated fat-continuous products
DE102008013490A1 (de) * 2008-03-10 2009-09-17 Bayer Technology Services Gmbh Verfahren zur Reinigung therapeutischer Proteine
US9469869B2 (en) * 2008-09-05 2016-10-18 The Regents Of The University Of Michigan Solution microarrays and uses thereof
CA2704702C (en) 2009-06-02 2018-06-12 Unilever Plc Aerated baked products
EP2548022B1 (de) * 2010-03-18 2016-03-16 The Regents Of The University Of Michigan Mehrphasen-mikroarrays und verwendungen davon
MX2013001718A (es) 2010-08-12 2013-10-28 Unilever Nv Composiciones de cuidado oral.
US20120040396A1 (en) * 2010-08-16 2012-02-16 Amyris, Inc. Methods for purifying bio-organic compounds
WO2012022749A1 (en) 2010-08-20 2012-02-23 Unilever Plc Hair treatment composition
EA023709B1 (ru) 2010-10-04 2016-07-29 Унилевер Н.В. Способ получения пищевого газового гидрата
US20130216655A1 (en) 2010-10-20 2013-08-22 Andrew Richard Cox Foaming agents comprising hydrophobin
CN101955509A (zh) * 2010-11-03 2011-01-26 温州大学 萃取酵母菌中的谷胱甘肽的方法
MX2013011906A (es) * 2011-04-15 2013-11-21 Danisco Us Inc Metodos para purificar hidrofobina.
FI20115704L (fi) 2011-07-01 2013-01-02 Teknologian Tutkimuskeskus Vtt Oy Vierasproteiinien tuoton parantaminen
CN103702754B (zh) * 2011-07-29 2017-04-26 东丽株式会社 助滤剂的制造方法
CN103687880B (zh) * 2011-08-10 2016-09-14 韩国科学技术院 具有增加的脂肪酶活性的两亲性肽-脂肪酶缀合物及其用途
EP2631296A1 (de) 2012-02-22 2013-08-28 Kimmo Koivu Verfahren zur Hydrophobinherstellung in Pflanzen und Verfahren zur Herstellung von Hydrophobinmultimeren in Pflanzen und Mikroben
FI127353B (en) * 2013-07-05 2018-04-13 Teknologian Tutkimuskeskus Vtt Oy Concentration and purification of hydrophobins and antibodies by a phase separation method
FI126194B (en) 2013-09-13 2016-08-15 Teknologian Tutkimuskeskus Vtt Oy Ways to form fibrous product
US8906972B1 (en) 2013-12-27 2014-12-09 King Fahd University Of Petroleum And Minerals Aqueous two phase (ATPSs) of pH-responsive poly(phosphonate-alt-sulfur dioxide) with PEG
WO2017072103A1 (en) * 2015-10-26 2017-05-04 Basf Se Oral care products and methods comprising hlps

Family Cites Families (9)

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Publication number Priority date Publication date Assignee Title
US5139943A (en) 1989-06-13 1992-08-18 Genencor International, Inc. Processes for the recovery of microbially produced chymosin
DE4001238A1 (de) * 1990-01-18 1991-07-25 Basf Ag Verfahren zur anreicherung bzw. reinigung von biomolekuelen
SE9003534D0 (sv) * 1990-11-06 1990-11-06 Kabigen Ab A method for isolating and purifying peptides and proteins
EP0574050A1 (de) 1992-05-19 1993-12-15 Gist-Brocades N.V. Trennung und Reinigung von Fermentationsprodukt in grossen Massstab
DK0673427T3 (da) 1992-07-08 2003-07-21 Unilever Nv Enzymatisk proces under anvendelse af enzymer immobiliseret på cellevæggen af en eukaryotisk mikrobiel celle ved fremstilling af et fusionsprotein
WO1994004673A1 (en) * 1992-08-19 1994-03-03 Alko Group Ltd. Fungal promoters active in the presence of glucose
AU6139794A (en) 1993-02-10 1994-08-29 Unilever Plc Immobilized proteins with specific binding capacities and their use in processes and products
US5882520A (en) * 1995-10-26 1999-03-16 The University Of Montana Use of arabinogalactan in aqueous two phase extractions
JP5483301B2 (ja) * 2005-06-03 2014-05-07 Ｊｘ日鉱日石エネルギー株式会社 緩衝器用油圧作動油組成物

Also Published As

Publication number Publication date
IL145337A0 (en) 2002-06-30
AU3562100A (en) 2000-10-16
NO20014534D0 (no) 2001-09-18
EP1163260B1 (de) 2009-05-20
AU778477B2 (en) 2004-12-09
US20060084164A1 (en) 2006-04-20
ATE431834T1 (de) 2009-06-15
JP4570787B2 (ja) 2010-10-27
KR100819187B1 (ko) 2008-04-04
DK1163260T3 (da) 2009-08-17
CN1249079C (zh) 2006-04-05
JP2002543766A (ja) 2002-12-24
CN1357005A (zh) 2002-07-03
WO2000058342A1 (en) 2000-10-05
US7060669B1 (en) 2006-06-13
EP1163260A1 (de) 2001-12-19
CA2367826A1 (en) 2000-10-05
KR20010108400A (ko) 2001-12-07
IL145337A (en) 2009-09-22
US7335492B2 (en) 2008-02-26
NZ514891A (en) 2003-10-31
NO20014534L (no) 2001-11-26

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Legal Events

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