DK1163260T3 - Fremgangsmåde til adskillelse af proteiner - Google Patents

Fremgangsmåde til adskillelse af proteiner

Info

Publication number
DK1163260T3
DK1163260T3 DK00914217T DK00914217T DK1163260T3 DK 1163260 T3 DK1163260 T3 DK 1163260T3 DK 00914217 T DK00914217 T DK 00914217T DK 00914217 T DK00914217 T DK 00914217T DK 1163260 T3 DK1163260 T3 DK 1163260T3
Authority
DK
Denmark
Prior art keywords
proteins
separating proteins
atps
protein
partitioning
Prior art date
Application number
DK00914217T
Other languages
English (en)
Inventor
Merja Penttilae
Tiina Nakari-Setaelae
Richard Fagerstroem
Maria-Regina Kula
Klaus Selber
Markus Linder
Folke Tjerneld
Original Assignee
Valtion Teknillinen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from FI990667A external-priority patent/FI990667A0/fi
Application filed by Valtion Teknillinen filed Critical Valtion Teknillinen
Application granted granted Critical
Publication of DK1163260T3 publication Critical patent/DK1163260T3/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/16Extraction; Separation; Purification by chromatography
    • C07K1/20Partition-, reverse-phase or hydrophobic interaction chromatography
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K7/00Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04Linear peptides containing only normal peptide links
    • C07K7/06Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K7/00Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04Linear peptides containing only normal peptide links
    • C07K7/08Linear peptides containing only normal peptide links having 12 to 20 amino acids
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
DK00914217T 1999-03-25 2000-03-24 Fremgangsmåde til adskillelse af proteiner DK1163260T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FI990667A FI990667A0 (fi) 1999-03-25 1999-03-25 Menetelmä molekyylien erottamiseksi
FI991782 1999-08-20
PCT/FI2000/000249 WO2000058342A1 (en) 1999-03-25 2000-03-24 Process for partitioning of proteins

Publications (1)

Publication Number Publication Date
DK1163260T3 true DK1163260T3 (da) 2009-08-17

Family

ID=26160722

Family Applications (1)

Application Number Title Priority Date Filing Date
DK00914217T DK1163260T3 (da) 1999-03-25 2000-03-24 Fremgangsmåde til adskillelse af proteiner

Country Status (14)

Country Link
US (2) US7060669B1 (da)
EP (1) EP1163260B1 (da)
JP (1) JP4570787B2 (da)
KR (1) KR100819187B1 (da)
CN (1) CN1249079C (da)
AT (1) ATE431834T1 (da)
AU (1) AU778477B2 (da)
CA (1) CA2367826A1 (da)
DE (1) DE60042235D1 (da)
DK (1) DK1163260T3 (da)
IL (2) IL145337A0 (da)
NO (1) NO20014534L (da)
NZ (1) NZ514891A (da)
WO (1) WO2000058342A1 (da)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI120310B (fi) * 2001-02-13 2009-09-15 Valtion Teknillinen Parannettu menetelmä erittyvien proteiinien tuottamiseksi sienissä
FI116198B (fi) 2001-07-16 2005-10-14 Valtion Teknillinen Polypeptidien immobilisointimenetelmä
MX2007000978A (es) 2004-07-27 2007-04-10 Unilever Nv Productos alimenticios aireados que contienen hidrofobina.
US7241734B2 (en) 2004-08-18 2007-07-10 E. I. Du Pont De Nemours And Company Thermophilic hydrophobin proteins and applications for surface modification
US7147912B2 (en) 2004-08-18 2006-12-12 E. I. Du Pont De Nemours And Company Amphipathic proteinaceous coating on nanoporous polymer
DE102005005737A1 (de) * 2005-02-07 2006-08-17 Basf Ag Neue Hydrophobinfusionsproteine, deren Herstellung und Verwendung
CA2596661C (en) 2005-02-07 2013-12-10 Basf Aktiengesellschaft Novel hydrophobin fusion products, production and use thereof
MX2007012021A (es) 2005-03-31 2008-03-14 Basf Ag El uso de polipeptidos como promotores de adhesion.
US20100170142A1 (en) * 2005-04-01 2010-07-08 Basf Aktiengesellschaft Use of Proteins as Demulsifying Agents
DE502006005460D1 (de) 2005-04-01 2010-01-07 Basf Se Bohrspülung enthaltend hydrophobin
US8535535B2 (en) * 2005-04-01 2013-09-17 Basf Se Use of hydrophobin as a phase stabilizer
DE102005027139A1 (de) * 2005-06-10 2006-12-28 Basf Ag Neue Cystein-verarmte Hydrophobinfusionsproteine, deren Herstellung und Verwendung
PT1926398E (pt) 2005-09-23 2011-02-15 Unilever Nv Produtos aerados com teor reduzido de creme
WO2007039065A1 (en) 2005-09-23 2007-04-12 Unilever Plc Low ph aerated products
DE102005048720A1 (de) 2005-10-12 2007-04-19 Basf Ag Verwendung von Proteinen als Antischaum-Komponente in Kraftstoffen
ATE394935T1 (de) 2005-12-21 2008-05-15 Unilever Nv Gefrorene belüftete süssspeisen
US8263343B2 (en) 2005-12-22 2012-09-11 Ge Healthcare Bio-Science Ab Preparation of biomolecules
DE602007009795D1 (de) * 2006-01-31 2010-11-25 Unilever Nv Mit luft durchsetzte zusammensetzungen mit hydrophobin
ATE529712T1 (de) 2006-08-15 2011-11-15 Basf Se Verfahren zur herstellung von trockenen freifliessenden hydrophobinzubereitungen
JP5245227B2 (ja) * 2006-08-23 2013-07-24 三菱瓦斯化学株式会社 疎水性化合物の起泡による分離法
CN100453642C (zh) * 2006-10-10 2009-01-21 山西职工医学院 一种过氧化物酶的提取方法
KR100836171B1 (ko) 2007-01-19 2008-06-09 인하대학교 산학협력단 분배 향상 물질이 접합된 생물물질을 이용한 수성이상계추출법에서의 친화성 분리 방법
KR100859791B1 (ko) * 2007-05-23 2008-09-23 인하대학교 산학협력단 상 형성 물질과 결합된 분자인식 리간드를 이용하여 상형성과 분자특이적 분리를 동시에 수행하는 방법
ES2319844B1 (es) * 2007-09-12 2010-02-16 Universidad Miguel Hernandez De Elche (70%) Procedimiento de separacion de proteinas recombinantes en sistemas acuosos de dos fases.
BRPI0816256A2 (pt) * 2007-09-13 2015-03-17 Basf Se Uso de hidrofobina, e, processo para a preparação de um agente para a absorção melhorada de substâncias ativas no uso tópico
AU2008229927B2 (en) 2007-10-25 2009-08-06 Unilever Plc Aerated fat-continuous products
DE102008013490A1 (de) * 2008-03-10 2009-09-17 Bayer Technology Services Gmbh Verfahren zur Reinigung therapeutischer Proteine
WO2010027590A2 (en) * 2008-09-05 2010-03-11 The Regents Of The University Of Michigan Solution microarrays and uses thereof
CA2704702C (en) 2009-06-02 2018-06-12 Unilever Plc Aerated baked products
EP2548022B1 (en) * 2010-03-18 2016-03-16 The Regents Of The University Of Michigan Multiphase microarrays and uses thereof
BR112013003275A2 (pt) 2010-08-12 2016-06-14 Unilever Nv composição de tratamento oral
US20120040396A1 (en) * 2010-08-16 2012-02-16 Amyris, Inc. Methods for purifying bio-organic compounds
WO2012022749A1 (en) 2010-08-20 2012-02-23 Unilever Plc Hair treatment composition
ES2559517T3 (es) 2010-10-04 2016-02-12 Unilever N.V. Procedimiento de producción de un hidrato de gas comestible
EP2629629B1 (en) * 2010-10-20 2015-05-13 Unilever PLC Foaming agents comprising hydrophobin
CN101955509A (zh) * 2010-11-03 2011-01-26 温州大学 萃取酵母菌中的谷胱甘肽的方法
CA2832975A1 (en) * 2011-04-15 2012-10-18 Danisco Us Inc. Methods of purifying hydrophobin
FI20115704L (fi) * 2011-07-01 2013-01-02 Teknologian Tutkimuskeskus Vtt Oy Vierasproteiinien tuoton parantaminen
MY162541A (en) * 2011-07-29 2017-06-15 Toray Industries Method of manufacturing filter aid
KR101677959B1 (ko) * 2011-08-10 2016-11-21 한국과학기술원 리파아제 활성이 증대된 양친매성 펩타이드-리파아제 결합체 및 이의 용도
EP2631296A1 (en) 2012-02-22 2013-08-28 Kimmo Koivu A method for hydrophobin production in plants and methods to produce hydrophobin multimers in plants and microbes
FI127353B (en) * 2013-07-05 2018-04-13 Teknologian Tutkimuskeskus Vtt Oy Concentration and purification of hydrophobins and antibodies by a phase separation method
FI126194B (en) 2013-09-13 2016-08-15 Teknologian Tutkimuskeskus Vtt Oy Ways to form fibrous product
US8906972B1 (en) 2013-12-27 2014-12-09 King Fahd University Of Petroleum And Minerals Aqueous two phase (ATPSs) of pH-responsive poly(phosphonate-alt-sulfur dioxide) with PEG
MX2018005199A (es) * 2015-10-26 2018-07-06 Basf Se Productos para el cuidado bucal y metodos que comprenden hlps.

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5139943A (en) 1989-06-13 1992-08-18 Genencor International, Inc. Processes for the recovery of microbially produced chymosin
DE4001238A1 (de) * 1990-01-18 1991-07-25 Basf Ag Verfahren zur anreicherung bzw. reinigung von biomolekuelen
SE9003534D0 (sv) * 1990-11-06 1990-11-06 Kabigen Ab A method for isolating and purifying peptides and proteins
EP0574050A1 (en) 1992-05-19 1993-12-15 Gist-Brocades N.V. Large scale separation and purification of fermentation product
ES2196009T3 (es) 1992-07-08 2003-12-16 Unilever Nv Procedimiento para inmovilizar enzimas en la pared celular de una celula microbiana mediante la produccion de una proteina de fusion.
AU4712193A (en) 1992-08-19 1994-03-15 Alko Group Limited Fungal promoters active in the presence of glucose
EP0682710B1 (en) 1993-02-10 2003-10-29 Unilever Plc Isolation process using immobilized proteins with specific binding capacities
US5882520A (en) * 1995-10-26 1999-03-16 The University Of Montana Use of arabinogalactan in aqueous two phase extractions
JP5483301B2 (ja) * 2005-06-03 2014-05-07 Jx日鉱日石エネルギー株式会社 緩衝器用油圧作動油組成物

Also Published As

Publication number Publication date
AU3562100A (en) 2000-10-16
CA2367826A1 (en) 2000-10-05
WO2000058342A1 (en) 2000-10-05
EP1163260A1 (en) 2001-12-19
IL145337A (en) 2009-09-22
US20060084164A1 (en) 2006-04-20
US7060669B1 (en) 2006-06-13
NZ514891A (en) 2003-10-31
KR100819187B1 (ko) 2008-04-04
IL145337A0 (en) 2002-06-30
NO20014534D0 (no) 2001-09-18
CN1249079C (zh) 2006-04-05
KR20010108400A (ko) 2001-12-07
DE60042235D1 (de) 2009-07-02
JP2002543766A (ja) 2002-12-24
NO20014534L (no) 2001-11-26
JP4570787B2 (ja) 2010-10-27
CN1357005A (zh) 2002-07-03
US7335492B2 (en) 2008-02-26
ATE431834T1 (de) 2009-06-15
EP1163260B1 (en) 2009-05-20
AU778477B2 (en) 2004-12-09

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