MXPA02007664A - Separacion y representacion de proteinas. - Google Patents
Separacion y representacion de proteinas.Info
- Publication number
- MXPA02007664A MXPA02007664A MXPA02007664A MXPA02007664A MXPA02007664A MX PA02007664 A MXPA02007664 A MX PA02007664A MX PA02007664 A MXPA02007664 A MX PA02007664A MX PA02007664 A MXPA02007664 A MX PA02007664A MX PA02007664 A MXPA02007664 A MX PA02007664A
- Authority
- MX
- Mexico
- Prior art keywords
- separation
- present
- display
- protein separation
- protein
- Prior art date
Links
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/38—Flow patterns
- G01N30/46—Flow patterns using more than one column
- G01N30/461—Flow patterns using more than one column with serial coupling of separation columns
- G01N30/463—Flow patterns using more than one column with serial coupling of separation columns for multidimensional chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N2030/022—Column chromatography characterised by the kind of separation mechanism
- G01N2030/027—Liquid chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/50—Conditioning of the sorbent material or stationary liquid
- G01N30/52—Physical parameters
- G01N2030/524—Physical parameters structural properties
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8804—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 automated systems
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
- G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
- G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
- G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44795—Isoelectric focusing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/38—Flow patterns
- G01N30/46—Flow patterns using more than one column
- G01N30/461—Flow patterns using more than one column with serial coupling of separation columns
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/72—Mass spectrometers
- G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/80—Fraction collectors
- G01N30/82—Automatic means therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/86—Signal analysis
- G01N30/8651—Recording, data aquisition, archiving and storage
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/24—Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
- Y10T436/255—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.] including use of a solid sorbent, semipermeable membrane, or liquid extraction
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Hematology (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
La presente invencion se refiere a metodos de separacion de proteinas de multi-fase capaces de resolver y caracterizar grandes numeros de proteinas celulares, incluyendo metodos para facilitar eficazmente la transferencia de muestras de proteina entre las fases de separacion. En particular, la presente invencion proporciona un sistema automatizado para la separacion, identificacion, y caracterizacion de muestras de proteina. La presente invencion proporciona de esta manera metodos mejorados para el analisis de muestras que contienen grandes numeros de proteinas.
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US18091100P | 2000-02-08 | 2000-02-08 | |
US23932500P | 2000-10-10 | 2000-10-10 | |
US23932600P | 2000-10-10 | 2000-10-10 | |
US25944801P | 2001-01-03 | 2001-01-03 | |
PCT/US2001/003917 WO2001058925A2 (en) | 2000-02-08 | 2001-02-07 | Protein separation and display |
Publications (1)
Publication Number | Publication Date |
---|---|
MXPA02007664A true MXPA02007664A (es) | 2004-08-23 |
Family
ID=27497425
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MXPA02007664A MXPA02007664A (es) | 2000-02-08 | 2001-02-07 | Separacion y representacion de proteinas. |
Country Status (7)
Country | Link |
---|---|
US (1) | US20020098595A1 (es) |
EP (1) | EP1358202A2 (es) |
JP (1) | JP2004500567A (es) |
AU (1) | AU2001236726A1 (es) |
CA (1) | CA2400484A1 (es) |
MX (1) | MXPA02007664A (es) |
WO (1) | WO2001058925A2 (es) |
Families Citing this family (31)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20050230315A1 (en) * | 2003-01-13 | 2005-10-20 | Regents Of The University Of Michigan | Protein microarray system |
CA2400460C (en) | 2000-02-08 | 2009-01-06 | The Regents Of The University Of Michigan | Mapping of differential display of proteins |
US20030064527A1 (en) * | 2001-02-07 | 2003-04-03 | The Regents Of The University Of Michigan | Proteomic differential display |
US20040214233A1 (en) * | 2003-01-13 | 2004-10-28 | The Regents Of The University Of Michigan | Protein microarray system |
US6931325B2 (en) | 2001-02-07 | 2005-08-16 | Regents Of The University Of Michigan | Three dimensional protein mapping |
ATE327512T1 (de) * | 2001-12-08 | 2006-06-15 | Micromass Ltd | Massenspektrometrie-verfahren |
EP1391723B1 (en) * | 2002-08-16 | 2006-06-21 | Agilent Technologies Inc. a Delaware Corporation | Method and apparatus for analyzing a mixture of sample substances |
DE10393475B4 (de) * | 2002-10-09 | 2014-09-25 | Waters Technologies Corp. (N.D.Ges.D. Staates Delaware) | Verfahren und Vorrichtung zur Identifizierung von Verbindungen in einer Probe |
US20040137526A1 (en) * | 2002-10-15 | 2004-07-15 | The Regents Of The University Of Michigan | Multidimensional protein separation system |
US7648678B2 (en) | 2002-12-20 | 2010-01-19 | Dako Denmark A/S | Method and system for pretreatment of tissue slides |
US6969452B2 (en) * | 2003-02-28 | 2005-11-29 | Combisep, Inc. | Two-dimensional protein separations using chromatofocusing and multiplexed capillary gel electrophoresis |
US20040180449A1 (en) * | 2003-03-11 | 2004-09-16 | Gilson, Inc. | Multidimensional chromatography with ion exchange solid phase extraction |
GB2404193A (en) * | 2003-07-21 | 2005-01-26 | Amersham Biosciences Ab | Automated chromatography/mass spectrometry analysis |
GB2404194A (en) * | 2003-07-21 | 2005-01-26 | Amersham Biosciences Ab | Automated chromatography/mass spectrometry analysis |
BRPI0413076B8 (pt) * | 2003-07-29 | 2021-07-27 | Abbot Laboratories Gmbh | processo analítico para composições de pancreatina e comparáveis |
FR2859113B1 (fr) * | 2003-08-29 | 2006-02-17 | Centre Nat Rech Scient | Procede de separation bidimensionnelle par electrophorese capillaire realise dans un seul capillaire |
KR100516389B1 (ko) * | 2003-09-08 | 2005-09-23 | 한국과학기술원 | 작은 열 충격 단백질을 함유하는 단백질 분해 방지용조성물 및 이를 이용한 이차원 전기영동법 |
ATE443870T1 (de) | 2004-07-20 | 2009-10-15 | Symphogen As | Verfahren zur charakterisierung einer polyklonalen zellinie |
WO2006007850A1 (en) | 2004-07-20 | 2006-01-26 | Symphogen A/S | Anti-rhesus d recombinant polyclonal antibody and methods of manufacture |
WO2006133392A1 (en) * | 2005-06-08 | 2006-12-14 | Vanderbilt University | Sampling of blood analytes |
US20090136960A1 (en) * | 2006-03-24 | 2009-05-28 | The Regents Of The University Of Michigan | Methods and compositions for the identification of cancer markers |
EP2132217A2 (en) * | 2007-02-26 | 2009-12-16 | Alltech Associates Inc. | Ultra-fast chromatography |
WO2009036193A1 (en) * | 2007-09-11 | 2009-03-19 | Cancer Prevention And Cure, Ltd. | Identification of proteins in human serum indicative of pathologies of human lung tissues |
JP2011505560A (ja) * | 2007-11-30 | 2011-02-24 | ウオーターズ・テクノロジーズ・コーポレイシヨン | サンプル中におけるミネラルコルチコイドの存在または不存在の決定 |
US7834313B2 (en) * | 2008-08-08 | 2010-11-16 | Quest Diagnostics Investments Incorporated | Mass spectrometry assay for plasma-renin |
JP5569761B1 (ja) * | 2013-03-29 | 2014-08-13 | シャープ株式会社 | 分析方法 |
JP6127790B2 (ja) * | 2013-07-12 | 2017-05-17 | 株式会社島津製作所 | 液体クロマトグラフ用制御装置および制御方法 |
CN105363426B (zh) * | 2015-12-08 | 2018-10-16 | 复旦大学 | 一种介孔二氧化硅复合材料结合质谱鉴定肽段的方法 |
CN105536748B (zh) * | 2015-12-09 | 2018-10-16 | 复旦大学 | 一种纳米复合材料结合质谱鉴定磷酸化肽段的方法 |
CA3233933A1 (en) * | 2017-01-30 | 2018-08-02 | Regeneron Pharmaceuticals, Inc. | Compositions and methods for reducing bioburden in chromatography |
WO2021252990A1 (en) * | 2020-06-13 | 2021-12-16 | Complete Omics Inc. | Method and system for neoantigen analysis |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SE7908454L (sv) * | 1979-10-11 | 1981-04-12 | Pharmacia Fine Chemicals Ab | Medium for isoelektrisk fokusering |
US5451662A (en) * | 1987-10-23 | 1995-09-19 | Schering Corporation | Method of purifying protein |
EP0617048A1 (en) * | 1993-03-26 | 1994-09-28 | Waters Investments Limited | Method of capillary isoelectric focusing of proteins and peptides with fraction collection for post-run analysis |
US5498545A (en) * | 1994-07-21 | 1996-03-12 | Vestal; Marvin L. | Mass spectrometer system and method for matrix-assisted laser desorption measurements |
DE69627333T2 (de) * | 1995-06-26 | 2004-02-12 | PerSeptive Biosystems, Inc., Framingham | Automatisierte, kontinuierliche mehrdimensionale hochgeschwindigkeitsmolekularselektion und -analyse |
US5670054A (en) * | 1996-04-04 | 1997-09-23 | Warner Lambert Company | Method and system for identification, purification, and quantitation of reaction components |
EP0984978A4 (en) * | 1997-03-14 | 2002-01-09 | Transgenomic Inc | TAPE PATTERN DISPLAY OF POLYNUCLEOTIS SEPARATIONS |
US6534764B1 (en) * | 1999-06-11 | 2003-03-18 | Perseptive Biosystems | Tandem time-of-flight mass spectrometer with damping in collision cell and method for use |
US6545268B1 (en) * | 2000-04-10 | 2003-04-08 | Perseptive Biosystems | Preparation of ion pulse for time-of-flight and for tandem time-of-flight mass analysis |
-
2001
- 2001-02-07 AU AU2001236726A patent/AU2001236726A1/en not_active Abandoned
- 2001-02-07 JP JP2001558072A patent/JP2004500567A/ja not_active Withdrawn
- 2001-02-07 CA CA002400484A patent/CA2400484A1/en not_active Abandoned
- 2001-02-07 MX MXPA02007664A patent/MXPA02007664A/es unknown
- 2001-02-07 EP EP01908914A patent/EP1358202A2/en not_active Withdrawn
- 2001-02-07 WO PCT/US2001/003917 patent/WO2001058925A2/en not_active Application Discontinuation
- 2001-02-07 US US09/778,548 patent/US20020098595A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
WO2001058925A3 (en) | 2003-09-04 |
WO2001058925A2 (en) | 2001-08-16 |
US20020098595A1 (en) | 2002-07-25 |
EP1358202A2 (en) | 2003-11-05 |
AU2001236726A1 (en) | 2001-08-20 |
JP2004500567A (ja) | 2004-01-08 |
CA2400484A1 (en) | 2001-08-16 |
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