WO2002029396A3 - Verfahren und vorrichtung zur 2d-elektrophorese in grossen gelen - Google Patents

Verfahren und vorrichtung zur 2d-elektrophorese in grossen gelen Download PDF

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Publication number
WO2002029396A3
WO2002029396A3 PCT/DE2001/003869 DE0103869W WO0229396A3 WO 2002029396 A3 WO2002029396 A3 WO 2002029396A3 DE 0103869 W DE0103869 W DE 0103869W WO 0229396 A3 WO0229396 A3 WO 0229396A3
Authority
WO
WIPO (PCT)
Prior art keywords
electrophoresis
porous
dimension
protein
separating
Prior art date
Application number
PCT/DE2001/003869
Other languages
English (en)
French (fr)
Other versions
WO2002029396A2 (de
Inventor
Joachim Klose
Original Assignee
Protagen Ag
Joachim Klose
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Protagen Ag, Joachim Klose filed Critical Protagen Ag
Priority to CA002424298A priority Critical patent/CA2424298A1/en
Priority to JP2002532919A priority patent/JP2004510978A/ja
Priority to EP01986344A priority patent/EP1322950A2/de
Priority to US10/398,408 priority patent/US20040069631A1/en
Priority to AU2002220491A priority patent/AU2002220491A1/en
Publication of WO2002029396A2 publication Critical patent/WO2002029396A2/de
Publication of WO2002029396A3 publication Critical patent/WO2002029396A3/de

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/24—Extraction; Separation; Purification by electrochemical means
    • C07K1/26—Electrophoresis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/24—Extraction; Separation; Purification by electrochemical means
    • C07K1/26—Electrophoresis
    • C07K1/28—Isoelectric focusing
    • C07K1/285—Isoelectric focusing multi dimensional electrophoresis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Peptides Or Proteins (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

Die Erfindung bezieht sich auf ein Verfahren und eine Vorrichtung zur Auftrennung komplexer Proteingemische mit Hilfe der hochauflösenden zweidimensionalen Elektrophorese (2D-Elektrophorese, 2DE) in grossen Gelen. Das Wesen der Erfindung besteht darin, dass die Trennung der Proteine in der ersten Dimension in hohlen porösen proteindurchlässigen Materialien vorgenommen wird und in der zweiten Dimension die porösen Materialien unverändert in eine Glaskassette eingebracht werden, die ein Flachgel enthält, in das die Proteine während der Elektrophorese durch die Wand des porösen Materials wandern. Die 2DE bildet zusammen mit der Massenspektrometrie die Basistechnik z.B. für die Proteomanalyse, d.h. für die Auftrennung und Identifizierung des Gesamtproteins eines Zelltyps, Organs oder Organismus.
PCT/DE2001/003869 2000-10-05 2001-10-04 Verfahren und vorrichtung zur 2d-elektrophorese in grossen gelen WO2002029396A2 (de)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA002424298A CA2424298A1 (en) 2000-10-05 2001-10-04 Method and device for carrying out 2d electrophoresis in large gels
JP2002532919A JP2004510978A (ja) 2000-10-05 2001-10-04 大きなゲルの中で２ｄの電気泳動を行なう方法及び装置
EP01986344A EP1322950A2 (de) 2000-10-05 2001-10-04 Verfahren und vorrichtung zur 2d-elektrophorese in grossen gelen
US10/398,408 US20040069631A1 (en) 2000-10-05 2001-10-04 Method and device for carrying out 2d electrophoresis in large gels
AU2002220491A AU2002220491A1 (en) 2000-10-05 2001-10-04 Method and device for carrying out 2D electrophoresis in large gels

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10050838.3 2000-10-05
DE10050838A DE10050838A1 (de) 2000-10-05 2000-10-05 Verfahren und Vorrichtung zur 2D-Elektrophorese in großen Gelen

Publications (2)

Publication Number Publication Date
WO2002029396A2 WO2002029396A2 (de) 2002-04-11
WO2002029396A3 true WO2002029396A3 (de) 2002-12-05

Family

ID=7659706

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2001/003869 WO2002029396A2 (de) 2000-10-05 2001-10-04 Verfahren und vorrichtung zur 2d-elektrophorese in grossen gelen

Country Status (7)

Country Link
US (1) US20040069631A1 (de)
EP (1) EP1322950A2 (de)
JP (1) JP2004510978A (de)
AU (1) AU2002220491A1 (de)
CA (1) CA2424298A1 (de)
DE (1) DE10050838A1 (de)
WO (1) WO2002029396A2 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1353171A3 (de) * 2002-04-12 2004-11-03 Tecan Trading AG Streifenhalter, Kammer, Kassette und 2D-Gelelektrophoresen-Verfahren
WO2004023131A1 (de) * 2002-09-03 2004-03-18 Proteosys Ag Isoelektrische fokussierung auf immobilisierten ph-gradienten
DE102005041638A1 (de) * 2005-08-27 2007-03-01 Wita Gmbh 2 D-Gel-Elektrophorese
US20070209939A1 (en) * 2006-03-10 2007-09-13 Protein Forest, Inc. Two-dimensional transfer device
JP2007256037A (ja) * 2006-03-23 2007-10-04 Gunma Prefecture 二次元電気泳動システムにおける等電点電気泳動用ゲル
WO2013118775A1 (ja) * 2012-02-07 2013-08-15 シャープ株式会社 二次元電気泳動キット、二次元電気泳動キットの製造方法、製造方法及び二次元電気泳動チップ

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0393478A2 (de) * 1989-04-18 1990-10-24 Millipore Corporation Verfahren zur Übertragung eines Gels und Verbundwerkstoff
WO2001085830A1 (en) * 2000-05-05 2001-11-15 University Of Louisville Research Foundation, Inc. Improved electrophoresis gel support

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3795600A (en) * 1973-03-23 1974-03-05 Instrumentation Specialties Co Electrophoresis and method apparatus
US4747919A (en) * 1987-01-16 1988-05-31 Large Scale Biology Corporation Apparatus and method for electrophoresis in tubes
US5534121A (en) * 1994-05-16 1996-07-09 The United States Of America As Represented By The Department Of Health And Human Services Preparative two dimensional gel electrophoresis system
WO1996009537A1 (en) * 1994-09-19 1996-03-28 Novel Experimental Technology Plastic mold for electrophoresis gel

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0393478A2 (de) * 1989-04-18 1990-10-24 Millipore Corporation Verfahren zur Übertragung eines Gels und Verbundwerkstoff
WO2001085830A1 (en) * 2000-05-05 2001-11-15 University Of Louisville Research Foundation, Inc. Improved electrophoresis gel support

Also Published As

Publication number Publication date
US20040069631A1 (en) 2004-04-15
DE10050838A1 (de) 2002-04-25
AU2002220491A1 (en) 2002-04-15
JP2004510978A (ja) 2004-04-08
CA2424298A1 (en) 2003-04-04
EP1322950A2 (de) 2003-07-02
WO2002029396A2 (de) 2002-04-11

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