MXPA02007665A - Trazado de despliegue diferencial de proteinas. - Google Patents

Trazado de despliegue diferencial de proteinas.

Info

Publication number
MXPA02007665A
MXPA02007665A MXPA02007665A MXPA02007665A MXPA02007665A MX PA02007665 A MXPA02007665 A MX PA02007665A MX PA02007665 A MXPA02007665 A MX PA02007665A MX PA02007665 A MXPA02007665 A MX PA02007665A MX PA02007665 A MXPA02007665 A MX PA02007665A
Authority
MX
Mexico
Prior art keywords
proteins
present
mapping
methods
differential
Prior art date
Application number
MXPA02007665A
Other languages
English (en)
Inventor
Bathsheba E Chong
Original Assignee
Univ Michigan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Michigan filed Critical Univ Michigan
Publication of MXPA02007665A publication Critical patent/MXPA02007665A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N2030/022—Column chromatography characterised by the kind of separation mechanism
    • G01N2030/027—Liquid chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • G01N30/52—Physical parameters
    • G01N2030/524—Physical parameters structural properties
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/461—Flow patterns using more than one column with serial coupling of separation columns
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/80—Fraction collectors
    • G01N30/82—Automatic means therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/86—Signal analysis
    • G01N30/8651—Recording, data aquisition, archiving and storage

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La presente invencion se refiere a metodos y sistemas de separacion de proteina capaces de resolver y caracterizar grandes numeros de proteinas celulares. En particular, la presente invencion proporciona un nuevo sistema de trazado de masa y metodos para el despliegue diferencial de proteinas. La presente invencion proporciona ademas nuevos metodos para desplegar expresion de proteina diferencial entre dos muestras. En particular, la presente invencion proporciona un nuevo metodo para trazar la expresion diferencial de proteinas en celulas cancerosas, pre-cancerosas y no cancerosas.
MXPA02007665A 2000-02-08 2001-02-07 Trazado de despliegue diferencial de proteinas. MXPA02007665A (es)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US18091100P 2000-02-08 2000-02-08
US23932600P 2000-10-10 2000-10-10
US23932500P 2000-10-10 2000-10-10
US25944801P 2001-01-03 2001-01-03
US25981601P 2001-01-04 2001-01-04
PCT/US2001/003887 WO2001059460A2 (en) 2000-02-08 2001-02-07 Mapping of differential display of proteins

Publications (1)

Publication Number Publication Date
MXPA02007665A true MXPA02007665A (es) 2004-08-23

Family

ID=27539042

Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA02007665A MXPA02007665A (es) 2000-02-08 2001-02-07 Trazado de despliegue diferencial de proteinas.

Country Status (7)

Country Link
US (1) US7069151B2 (es)
EP (1) EP1256001A2 (es)
JP (1) JP2003534533A (es)
AU (1) AU2001234880A1 (es)
CA (1) CA2400460C (es)
MX (1) MXPA02007665A (es)
WO (1) WO2001059460A2 (es)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080096284A1 (en) * 2000-02-08 2008-04-24 Regents Of The University Of Michigan Protein separation and analysis
EP1385869A2 (en) * 2000-02-08 2004-02-04 The Regents Of The University Of Michigan Protein mapping
US20030064527A1 (en) * 2001-02-07 2003-04-03 The Regents Of The University Of Michigan Proteomic differential display
US20050230315A1 (en) * 2003-01-13 2005-10-20 Regents Of The University Of Michigan Protein microarray system
US6931325B2 (en) 2001-02-07 2005-08-16 Regents Of The University Of Michigan Three dimensional protein mapping
AU2002356910A1 (en) * 2001-11-05 2003-07-09 Irm, Llc Methods and devices for proteomics data complexity reduction
DE60328511D1 (de) * 2002-10-30 2009-09-03 Varian Inc Verwendung von temperatur- und strömungsprofilen bei analytischen prozessen auf gradientenelutionsbasis
ATE405592T1 (de) * 2003-01-13 2008-09-15 Univ Michigan Verfahren zur bildung eines protein- mikroarraysystems
US20050009114A1 (en) * 2003-05-15 2005-01-13 Board Of Regents, The University Of Texas System Identification and quantification of organ-specific proteins derived from human allogeneic cells using proteomics
US7425700B2 (en) * 2003-05-22 2008-09-16 Stults John T Systems and methods for discovery and analysis of markers
US20040236603A1 (en) * 2003-05-22 2004-11-25 Biospect, Inc. System of analyzing complex mixtures of biological and other fluids to identify biological state information
GB0322356D0 (en) * 2003-09-24 2003-10-22 Micromass Ltd Mass spectrometer
US20050244973A1 (en) * 2004-04-29 2005-11-03 Predicant Biosciences, Inc. Biological patterns for diagnosis and treatment of cancer
CA2574062A1 (en) 2004-07-20 2006-01-26 Symphogen A/S Anti-rhesus d recombinant polyclonal antibody and methods of manufacture
RU2426795C2 (ru) 2004-07-20 2011-08-20 Симфоген А/С Способ структурной характеристики рекомбинантного поликлонального белка или поликлональной клеточной линии
US20060214104A1 (en) * 2004-10-26 2006-09-28 Invitrogen Corporation Compositions and methods for analyzing biomolecules using mass spectroscopy
WO2006134056A1 (en) * 2005-06-14 2006-12-21 Cellzome Ag Process for the identification of novel enzyme interacting compounds
US20090136960A1 (en) * 2006-03-24 2009-05-28 The Regents Of The University Of Michigan Methods and compositions for the identification of cancer markers
US20110171619A1 (en) * 2009-05-28 2011-07-14 Daniel Leo Sweeney Representation of molecules as sets of masses of complementary subgroups and contiguous complementary subgroups
EP3746794A1 (en) 2018-01-31 2020-12-09 Regeneron Pharmaceuticals, Inc. A dual-column lc-ms system and methods of use thereof
EP4010456A4 (en) 2019-08-05 2023-09-13 Seer, Inc. SYSTEMS AND METHODS FOR SAMPLE PREPARATION, DATA GENERATION AND ANALYSIS OF CORONA PROTEIN

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0617048A1 (en) 1993-03-26 1994-09-28 Waters Investments Limited Method of capillary isoelectric focusing of proteins and peptides with fraction collection for post-run analysis
US5498545A (en) 1994-07-21 1996-03-12 Vestal; Marvin L. Mass spectrometer system and method for matrix-assisted laser desorption measurements
DE69627333T2 (de) 1995-06-26 2004-02-12 PerSeptive Biosystems, Inc., Framingham Automatisierte, kontinuierliche mehrdimensionale hochgeschwindigkeitsmolekularselektion und -analyse
US5670054A (en) 1996-04-04 1997-09-23 Warner Lambert Company Method and system for identification, purification, and quantitation of reaction components
JP2001504235A (ja) 1997-03-14 2001-03-27 トランスジエノミツク・インコーポレーテツド ポリヌクレオチド分離のバンド列の表示
NZ516848A (en) * 1997-06-20 2004-03-26 Ciphergen Biosystems Inc Retentate chromatography apparatus with applications in biology and medicine
EP1153300A2 (en) * 1999-02-16 2001-11-14 THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES Lcm (laser capture microdissection) for cellular protein analysis
US6534764B1 (en) * 1999-06-11 2003-03-18 Perseptive Biosystems Tandem time-of-flight mass spectrometer with damping in collision cell and method for use
EP1385869A2 (en) 2000-02-08 2004-02-04 The Regents Of The University Of Michigan Protein mapping
MXPA02007664A (es) 2000-02-08 2004-08-23 Univ Michigan Separacion y representacion de proteinas.
CA2301451A1 (en) * 2000-03-20 2001-09-21 Thang T. Pham Method for analysis of analytes by mass spectrometry
CA2446337A1 (en) 2001-05-02 2002-11-07 Regents Of The University Of Michigan Differential display protein maps

Also Published As

Publication number Publication date
AU2001234880A1 (en) 2001-08-20
US20020039747A1 (en) 2002-04-04
JP2003534533A (ja) 2003-11-18
CA2400460C (en) 2009-01-06
WO2001059460A3 (en) 2002-02-07
EP1256001A2 (en) 2002-11-13
CA2400460A1 (en) 2001-08-16
US7069151B2 (en) 2006-06-27
WO2001059460A2 (en) 2001-08-16

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