ES2168300T3 - Separacion de proteinas. - Google Patents

Separacion de proteinas.

Info

Publication number
ES2168300T3
ES2168300T3 ES94920412T ES94920412T ES2168300T3 ES 2168300 T3 ES2168300 T3 ES 2168300T3 ES 94920412 T ES94920412 T ES 94920412T ES 94920412 T ES94920412 T ES 94920412T ES 2168300 T3 ES2168300 T3 ES 2168300T3
Authority
ES
Spain
Prior art keywords
pct
protein
proteins
sec
date
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94920412T
Other languages
English (en)
Inventor
Birgitte Mahler Nilsson
Mads Aage Laustsen
Anders Rancke-Madsen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novozymes AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes AS filed Critical Novozymes AS
Application granted granted Critical
Publication of ES2168300T3 publication Critical patent/ES2168300T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C07K1/306—Extraction; Separation; Purification by precipitation by crystallization
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Electrical Discharge Machining, Electrochemical Machining, And Combined Machining (AREA)

Abstract

LA PRESENTE INVENCION SE REFIERE A UN METODO DE SEPARACION DE UNA PROTEINA, EN PARTICULAR UNA ENZIMA, DE UNA SOLUCION ACUOSA DE PROTEINAS QUE CONSISTE (A) EN PROPORCIONAR UNA MEZCLA ACUOSA DE PROTEINAS CON UNA CONCENTRACION DE SAL A O INFERIOR A 1.5 MOLAR, A LA CUAL SE HA AÑADIDO UN POLIMERO HIDROSOLUBLE, Y (B) RECUPERARLA PROTEINA EN FORMA CRISTALINA.
ES94920412T 1993-07-09 1994-06-23 Separacion de proteinas. Expired - Lifetime ES2168300T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DK93830A DK83093D0 (da) 1993-07-09 1993-07-09 Fremgangsmaade

Publications (1)

Publication Number Publication Date
ES2168300T3 true ES2168300T3 (es) 2002-06-16

Family

ID=8098038

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94920412T Expired - Lifetime ES2168300T3 (es) 1993-07-09 1994-06-23 Separacion de proteinas.

Country Status (13)

Country Link
US (1) US5728559A (es)
EP (1) EP0707594B1 (es)
JP (1) JPH08512294A (es)
CN (1) CN1054159C (es)
AT (1) ATE209214T1 (es)
AU (1) AU7121494A (es)
BR (1) BR9407047A (es)
DE (1) DE69429172T2 (es)
DK (2) DK83093D0 (es)
ES (1) ES2168300T3 (es)
FI (1) FI112949B (es)
PT (1) PT707594E (es)
WO (1) WO1995001989A1 (es)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE310745T1 (de) * 1996-03-08 2005-12-15 Novozymes As Kristallisation eines proteins mit einem schwefelsalz
US6207437B1 (en) 1996-03-11 2001-03-27 Genencor International, Inc. Crystalline protease and method for producing same
EP0942921B1 (en) * 1996-03-14 2005-11-30 Novozymes A/S Increased yields of a crystallized protein by using a solid adsorption material
US6031082A (en) * 1996-03-14 2000-02-29 Novo Nordisk A/S Increased yields of a crystallized protein by using a solid adsorption material
ATE233784T1 (de) * 1996-03-15 2003-03-15 Novozymes As Verfahren zur proteinaufreinigung aus einer proteinhaltigen lösung
US20030022383A1 (en) * 1999-04-06 2003-01-30 Uab Research Foundation Method for screening crystallization conditions in solution crystal growth
MXPA01009999A (es) * 1999-04-06 2003-07-14 Uab Research Foundation Metodo para examinar las condiciones de cristalizacion en el crecimiento de cristales en solucion.
US7250305B2 (en) * 2001-07-30 2007-07-31 Uab Research Foundation Use of dye to distinguish salt and protein crystals under microcrystallization conditions
US7214540B2 (en) * 1999-04-06 2007-05-08 Uab Research Foundation Method for screening crystallization conditions in solution crystal growth
US7247490B2 (en) * 1999-04-06 2007-07-24 Uab Research Foundation Method for screening crystallization conditions in solution crystal growth
US7244396B2 (en) * 1999-04-06 2007-07-17 Uab Research Foundation Method for preparation of microarrays for screening of crystal growth conditions
EP1289629B1 (en) * 2000-05-18 2004-08-04 Novozymes A/S Microfiltration using activated carbon
US6663602B2 (en) 2000-06-16 2003-12-16 Novo Nordisk A/S Injection device
US7670429B2 (en) * 2001-04-05 2010-03-02 The California Institute Of Technology High throughput screening of crystallization of materials
AU2002349300A1 (en) * 2001-12-11 2003-06-23 Novozymes A/S Process for harvesting crystalline particles from fermentation broth
US7118891B2 (en) 2001-12-11 2006-10-10 Novozymes A/S Crystal harvest from fermentation broth
US20070026528A1 (en) * 2002-05-30 2007-02-01 Delucas Lawrence J Method for screening crystallization conditions in solution crystal growth
WO2004005898A1 (en) * 2002-07-10 2004-01-15 Uab Research Foundation Method for distinguishing between biomolecule and non-biomolecule crystals
US20040185449A1 (en) * 2003-03-20 2004-09-23 Quinn John J. Method for preparing assay samples
EP1664398A4 (en) * 2003-09-03 2009-08-26 Shmuel Bukshpan METHODS AND DEVICES FOR REALIZING RAPID CRYSTALLIZATION OF BIOLOGICAL MOLECULES
US7507552B1 (en) 2004-04-16 2009-03-24 Takeda San Diego, Inc. Crystallization of histone deacetylase 2
ATE444090T1 (de) 2004-10-21 2009-10-15 Novo Nordisk As Wählmechanismus für einen drehstift
CN101163514B (zh) 2005-04-24 2012-01-25 诺和诺德公司 注射装置
EP1996259B1 (en) * 2006-03-10 2012-08-15 Novo Nordisk A/S An injection device and a method of changing a cartridge in the device
EP1996260B1 (en) * 2006-03-10 2015-09-23 Novo Nordisk A/S An injection device having a gearing arrangement
EP2004671B1 (en) * 2006-03-30 2015-12-09 Novo Nordisk A/S Two-phase precipitation of proteins
US8226618B2 (en) 2006-05-16 2012-07-24 Novo Nordisk A/S Gearing mechanism for an injection device
PL2023982T3 (pl) * 2006-05-18 2012-11-30 Novo Nordisk As Urządzenie wstrzykujące ze środkami blokującymi
FR2902799B1 (fr) 2006-06-27 2012-10-26 Millipore Corp Procede et unite de preparation d'un echantillon pour l'analyse microbiologique d'un liquide
US8569464B2 (en) 2006-12-21 2013-10-29 Emd Millipore Corporation Purification of proteins
US20100267933A1 (en) 2006-12-21 2010-10-21 Moya Wilson Purification of proteins
US8362217B2 (en) 2006-12-21 2013-01-29 Emd Millipore Corporation Purification of proteins
DK2125865T3 (da) 2007-03-15 2014-10-06 Novozymes As Opløsning af proteasekrystaller i fermenteringssuppe
RU2468829C2 (ru) * 2007-03-23 2012-12-10 Ново Нордиск А/С Инъекционное устройство, содержащее стопорную гайку
DE102008029401A1 (de) * 2008-06-23 2009-12-24 Bayer Technology Services Gmbh Verfahren zur Kristallisation von Peptiden und Proteinen
DK2370561T3 (da) 2008-12-16 2019-10-21 Emd Millipore Corp Omrøringstankreaktor og fremgangsmåde
EP3597671B1 (en) 2010-05-17 2022-09-21 EMD Millipore Corporation Stimulus responsive polymers for the purification of biomolecules
MX352973B (es) 2011-09-22 2017-12-15 Danisco Us Inc Actividad de adnasa endogena para reducir el contenido de adn.
JP6069351B2 (ja) 2011-12-29 2017-02-01 ノボ・ノルデイスク・エー／エス ダイアルアップ／ダイアルダウン投与機構を有するねじりバネ式ねじ巻き自動注射器
WO2023200284A1 (ko) * 2022-04-15 2023-10-19 재단법인대구경북과학기술원 생체 시료 내 지질 연관 단백질의 질량 분석을 위한 지질 연관 단백질의 정제 및 농축 방법

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4340676A (en) * 1980-09-24 1982-07-20 University Patents, Inc. Method of crystallizing ribulose, 1,5-bisphosphate carboxylase/oxygenase from photosynthetic organisms, particularly plant leaves
US4400471A (en) * 1980-11-03 1983-08-23 Johal Sarjit S Preparation and crystallization of Fraction I protein from plant sources
US5244800A (en) * 1990-04-27 1993-09-14 The Uab Research Foundation Crystals of human complement factor d that are triclinic

Also Published As

Publication number Publication date
WO1995001989A1 (en) 1995-01-19
FI960073A (fi) 1996-01-08
DE69429172D1 (de) 2002-01-03
EP0707594B1 (en) 2001-11-21
CN1054159C (zh) 2000-07-05
BR9407047A (pt) 1996-08-13
JPH08512294A (ja) 1996-12-24
PT707594E (pt) 2002-05-31
CN1126477A (zh) 1996-07-10
AU7121494A (en) 1995-02-06
ATE209214T1 (de) 2001-12-15
EP0707594A1 (en) 1996-04-24
DE69429172T2 (de) 2002-07-11
FI960073A0 (fi) 1996-01-08
FI112949B (fi) 2004-02-13
DK83093D0 (da) 1993-07-09
US5728559A (en) 1998-03-17
DK0707594T3 (da) 2002-05-21

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