ES2168300T3 - Separacion de proteinas. - Google Patents

Separacion de proteinas.

Info

Publication number
ES2168300T3
ES2168300T3 ES94920412T ES94920412T ES2168300T3 ES 2168300 T3 ES2168300 T3 ES 2168300T3 ES 94920412 T ES94920412 T ES 94920412T ES 94920412 T ES94920412 T ES 94920412T ES 2168300 T3 ES2168300 T3 ES 2168300T3
Authority
ES
Spain
Prior art keywords
pct
protein
proteins
sec
date
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94920412T
Other languages
English (en)
Inventor
Birgitte Mahler Nilsson
Mads Aage Laustsen
Anders Rancke-Madsen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novozymes AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes AS filed Critical Novozymes AS
Application granted granted Critical
Publication of ES2168300T3 publication Critical patent/ES2168300T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C07K1/306—Extraction; Separation; Purification by precipitation by crystallization
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Electrical Discharge Machining, Electrochemical Machining, And Combined Machining (AREA)

Abstract

LA PRESENTE INVENCION SE REFIERE A UN METODO DE SEPARACION DE UNA PROTEINA, EN PARTICULAR UNA ENZIMA, DE UNA SOLUCION ACUOSA DE PROTEINAS QUE CONSISTE (A) EN PROPORCIONAR UNA MEZCLA ACUOSA DE PROTEINAS CON UNA CONCENTRACION DE SAL A O INFERIOR A 1.5 MOLAR, A LA CUAL SE HA AÑADIDO UN POLIMERO HIDROSOLUBLE, Y (B) RECUPERARLA PROTEINA EN FORMA CRISTALINA.
ES94920412T 1993-07-09 1994-06-23 Separacion de proteinas. Expired - Lifetime ES2168300T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DK93830A DK83093D0 (da) 1993-07-09 1993-07-09 Fremgangsmaade

Publications (1)

Publication Number Publication Date
ES2168300T3 true ES2168300T3 (es) 2002-06-16

Family

ID=8098038

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94920412T Expired - Lifetime ES2168300T3 (es) 1993-07-09 1994-06-23 Separacion de proteinas.

Country Status (13)

Country Link
US (1) US5728559A (es)
EP (1) EP0707594B1 (es)
JP (1) JPH08512294A (es)
CN (1) CN1054159C (es)
AT (1) ATE209214T1 (es)
AU (1) AU7121494A (es)
BR (1) BR9407047A (es)
DE (1) DE69429172T2 (es)
DK (2) DK83093D0 (es)
ES (1) ES2168300T3 (es)
FI (1) FI112949B (es)
PT (1) PT707594E (es)
WO (1) WO1995001989A1 (es)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000506145A (ja) * 1996-03-08 2000-05-23 ノボ ノルディスク アクティーゼルスカブ 硫化塩によるタンパク質の結晶化
US6207437B1 (en) * 1996-03-11 2001-03-27 Genencor International, Inc. Crystalline protease and method for producing same
CN1216898C (zh) * 1996-03-14 2005-08-31 诺沃奇梅兹有限公司 利用固体吸附材料以提高的产率结晶蛋白质的方法
US6031082A (en) * 1996-03-14 2000-02-29 Novo Nordisk A/S Increased yields of a crystallized protein by using a solid adsorption material
EP0894091B1 (en) * 1996-03-15 2003-03-05 Novozymes A/S Method for purification of a protein from a protein containing solution
WO2000060345A1 (en) * 1999-04-06 2000-10-12 University Of Alabama At Birmingham Research Foundation Method for screening crystallization conditions in solution crystal growth
US7250305B2 (en) * 2001-07-30 2007-07-31 Uab Research Foundation Use of dye to distinguish salt and protein crystals under microcrystallization conditions
US7214540B2 (en) * 1999-04-06 2007-05-08 Uab Research Foundation Method for screening crystallization conditions in solution crystal growth
US7244396B2 (en) * 1999-04-06 2007-07-17 Uab Research Foundation Method for preparation of microarrays for screening of crystal growth conditions
US7247490B2 (en) * 1999-04-06 2007-07-24 Uab Research Foundation Method for screening crystallization conditions in solution crystal growth
US20020164812A1 (en) * 1999-04-06 2002-11-07 Uab Research Foundation Method for screening crystallization conditions in solution crystal growth
WO2001087468A1 (en) * 2000-05-18 2001-11-22 Novozymes A/S Microfiltration using activated carbon
US6663602B2 (en) * 2000-06-16 2003-12-16 Novo Nordisk A/S Injection device
US7670429B2 (en) * 2001-04-05 2010-03-02 The California Institute Of Technology High throughput screening of crystallization of materials
EP1456361B1 (en) * 2001-12-11 2012-02-29 Novozymes A/S Process for harvesting crystalline particles from fermentation broth
US7118891B2 (en) 2001-12-11 2006-10-10 Novozymes A/S Crystal harvest from fermentation broth
US20070026528A1 (en) * 2002-05-30 2007-02-01 Delucas Lawrence J Method for screening crystallization conditions in solution crystal growth
US20040007672A1 (en) * 2002-07-10 2004-01-15 Delucas Lawrence J. Method for distinguishing between biomolecule and non-biomolecule crystals
US20040185449A1 (en) * 2003-03-20 2004-09-23 Quinn John J. Method for preparing assay samples
US7459021B2 (en) * 2003-09-03 2008-12-02 Shmuel Bukshpan Methods and apparatus for rapid crystallization of biomolecules
US7507552B1 (en) 2004-04-16 2009-03-24 Takeda San Diego, Inc. Crystallization of histone deacetylase 2
PL1804865T3 (pl) 2004-10-21 2010-03-31 Novo Nordisk As Mechanizm wkręcania dla nawijanych penów
EP1877121B1 (en) * 2005-04-24 2015-09-23 Novo Nordisk A/S Injection device
US8298194B2 (en) * 2006-03-10 2012-10-30 Novo Nordisk A/S Injection device and a method of changing a cartridge in the device
CN101400394B (zh) * 2006-03-10 2012-07-04 诺沃-诺迪斯克有限公司 具有传动部件的注射装置
EP2004671B1 (en) * 2006-03-30 2015-12-09 Novo Nordisk A/S Two-phase precipitation of proteins
DE602007004972D1 (de) 2006-05-16 2010-04-08 Novo Nordisk As Getriebemechanismus für ein injektionsgerät
BRPI0712028A2 (pt) * 2006-05-18 2011-12-20 Novo Nordisk As dispositivo de injenção com um meio de travamento de modo
FR2902799B1 (fr) 2006-06-27 2012-10-26 Millipore Corp Procede et unite de preparation d'un echantillon pour l'analyse microbiologique d'un liquide
US8569464B2 (en) 2006-12-21 2013-10-29 Emd Millipore Corporation Purification of proteins
US8362217B2 (en) 2006-12-21 2013-01-29 Emd Millipore Corporation Purification of proteins
WO2008079280A1 (en) 2006-12-21 2008-07-03 Millipore Corporation Purification of proteins
DK2125865T3 (da) 2007-03-15 2014-10-06 Novozymes As Opløsning af proteasekrystaller i fermenteringssuppe
US8353878B2 (en) * 2007-03-23 2013-01-15 Novo Nordisk A/S Injection device comprising a locking nut
DE102008029401A1 (de) * 2008-06-23 2009-12-24 Bayer Technology Services Gmbh Verfahren zur Kristallisation von Peptiden und Proteinen
US20100190963A1 (en) 2008-12-16 2010-07-29 Millipore Corporation Stirred Tank Reactor And Method
KR101827855B1 (ko) 2010-05-17 2018-02-12 이엠디 밀리포어 코포레이션 생체분자 정제용 자극 반응성 중합체
AR087980A1 (es) 2011-09-22 2014-04-30 Danisco Us Inc Actividad de dnasa endogena para reducir el contenido de adn
EP2797650A2 (en) 2011-12-29 2014-11-05 Novo Nordisk A/S Torsion-spring based wind-up autoinjector pen with dial-up/dial-down dosing mechanism
WO2023200284A1 (ko) * 2022-04-15 2023-10-19 재단법인대구경북과학기술원 생체 시료 내 지질 연관 단백질의 질량 분석을 위한 지질 연관 단백질의 정제 및 농축 방법

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4340676A (en) * 1980-09-24 1982-07-20 University Patents, Inc. Method of crystallizing ribulose, 1,5-bisphosphate carboxylase/oxygenase from photosynthetic organisms, particularly plant leaves
US4400471A (en) * 1980-11-03 1983-08-23 Johal Sarjit S Preparation and crystallization of Fraction I protein from plant sources
US5244800A (en) * 1990-04-27 1993-09-14 The Uab Research Foundation Crystals of human complement factor d that are triclinic

Also Published As

Publication number Publication date
FI112949B (fi) 2004-02-13
EP0707594B1 (en) 2001-11-21
JPH08512294A (ja) 1996-12-24
PT707594E (pt) 2002-05-31
AU7121494A (en) 1995-02-06
FI960073A0 (fi) 1996-01-08
FI960073A (fi) 1996-01-08
DK83093D0 (da) 1993-07-09
DE69429172T2 (de) 2002-07-11
DE69429172D1 (de) 2002-01-03
BR9407047A (pt) 1996-08-13
EP0707594A1 (en) 1996-04-24
DK0707594T3 (da) 2002-05-21
WO1995001989A1 (en) 1995-01-19
US5728559A (en) 1998-03-17
CN1054159C (zh) 2000-07-05
ATE209214T1 (de) 2001-12-15
CN1126477A (zh) 1996-07-10

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