ATE233784T1 - Verfahren zur proteinaufreinigung aus einer proteinhaltigen lösung - Google Patents

Verfahren zur proteinaufreinigung aus einer proteinhaltigen lösung

Info

Publication number
ATE233784T1
ATE233784T1 AT97914167T AT97914167T ATE233784T1 AT E233784 T1 ATE233784 T1 AT E233784T1 AT 97914167 T AT97914167 T AT 97914167T AT 97914167 T AT97914167 T AT 97914167T AT E233784 T1 ATE233784 T1 AT E233784T1
Authority
AT
Austria
Prior art keywords
protein
solution containing
purification
cellulase
crystalline form
Prior art date
Application number
AT97914167T
Other languages
English (en)
Inventor
Anders Rancke-Madsen
Mads Aage Laustsen
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of ATE233784T1 publication Critical patent/ATE233784T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C07K1/306—Extraction; Separation; Purification by precipitation by crystallization
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification
    • Y10S435/816—Enzyme separation or purification by solubility
AT97914167T 1996-03-15 1997-03-14 Verfahren zur proteinaufreinigung aus einer proteinhaltigen lösung ATE233784T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK30596 1996-03-15
PCT/DK1997/000113 WO1997034919A1 (en) 1996-03-15 1997-03-14 Method for purification of a protein from a protein containing solution

Publications (1)

Publication Number Publication Date
ATE233784T1 true ATE233784T1 (de) 2003-03-15

Family

ID=8092063

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97914167T ATE233784T1 (de) 1996-03-15 1997-03-14 Verfahren zur proteinaufreinigung aus einer proteinhaltigen lösung

Country Status (10)

Country Link
US (1) US6087148A (de)
EP (1) EP0894091B1 (de)
JP (1) JP2000506534A (de)
CN (1) CN1216899C (de)
AT (1) ATE233784T1 (de)
AU (1) AU2151997A (de)
DE (1) DE69719497T2 (de)
DK (1) DK0894091T3 (de)
IN (1) IN190120B (de)
WO (1) WO1997034919A1 (de)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0894091B1 (de) * 1996-03-15 2003-03-05 Novozymes A/S Verfahren zur proteinaufreinigung aus einer proteinhaltigen lösung
AU757991B2 (en) * 1998-04-27 2003-03-13 Altus Pharmaceuticals, Inc. Stabilized protein crystals, formulations containing them and methods of making them
DE19859876A1 (de) * 1998-12-23 2000-06-29 Fraunhofer Ges Forschung Verfahren zur Kristallisation aus Lösungen
GB9910975D0 (en) 1999-05-13 1999-07-14 Univ Strathclyde Rapid dehydration of proteins
US6663602B2 (en) 2000-06-16 2003-12-16 Novo Nordisk A/S Injection device
DE60231534D1 (de) * 2001-10-25 2009-04-23 Kyowa Hakko Kogyo Kk Kristall von oxidiertem glutathion und verfahren zu dessen herstellung
JP4500683B2 (ja) * 2002-11-01 2010-07-14 バイエル・ヘルスケア・エルエルシー 巨大分子濃縮方法
ATE444090T1 (de) 2004-10-21 2009-10-15 Novo Nordisk As Wählmechanismus für einen drehstift
WO2007113188A1 (en) * 2006-03-30 2007-10-11 Novo Nordisk A/S Two-phase precipitation of proteins
DE602007004972D1 (de) 2006-05-16 2010-04-08 Novo Nordisk As Getriebemechanismus für ein injektionsgerät
AU2007253481B2 (en) 2006-05-18 2013-01-17 Novo Nordisk A/S An injection device with mode locking means
DE102008029401A1 (de) * 2008-06-23 2009-12-24 Bayer Technology Services Gmbh Verfahren zur Kristallisation von Peptiden und Proteinen
FR2943686B1 (fr) * 2009-03-30 2013-11-01 Roquette Freres Procede d'obtention d'une preparation de beta-amylases a partir des fractions solubles de plantes amidonnieres
JP6069351B2 (ja) 2011-12-29 2017-02-01 ノボ・ノルデイスク・エー／エス ダイアルアップ／ダイアルダウン投与機構を有するねじりバネ式ねじ巻き自動注射器
WO2023200284A1 (ko) * 2022-04-15 2023-10-19 재단법인대구경북과학기술원 생체 시료 내 지질 연관 단백질의 질량 분석을 위한 지질 연관 단백질의 정제 및 농축 방법
WO2024032881A1 (en) * 2022-08-10 2024-02-15 Arla Foods Amba Crystallisation of a protein capable of crystallizing under salting-in conditions using multiple protein feeds

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3664926A (en) * 1970-02-26 1972-05-23 Merck & Co Inc Crystallization of l-asparaginase
DE4001238A1 (de) * 1990-01-18 1991-07-25 Basf Ag Verfahren zur anreicherung bzw. reinigung von biomolekuelen
DK115890D0 (da) * 1990-05-09 1990-05-09 Novo Nordisk As Enzym
DK83093D0 (da) * 1993-07-09 1993-07-09 Novo Nordisk As Fremgangsmaade
EP0894091B1 (de) * 1996-03-15 2003-03-05 Novozymes A/S Verfahren zur proteinaufreinigung aus einer proteinhaltigen lösung

Also Published As

Publication number Publication date
EP0894091B1 (de) 2003-03-05
CN1213377A (zh) 1999-04-07
JP2000506534A (ja) 2000-05-30
US6087148A (en) 2000-07-11
DK0894091T3 (da) 2003-06-10
CN1216899C (zh) 2005-08-31
EP0894091A1 (de) 1999-02-03
DE69719497T2 (de) 2003-10-30
DE69719497D1 (de) 2003-04-10
IN190120B (de) 2003-06-14
WO1997034919A1 (en) 1997-09-25
AU2151997A (en) 1997-10-10

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