DE69719497D1 - Verfahren zur proteinaufreinigung aus einer proteinhaltigen lösung - Google Patents

Verfahren zur proteinaufreinigung aus einer proteinhaltigen lösung

Info

Publication number
DE69719497D1
DE69719497D1 DE69719497T DE69719497T DE69719497D1 DE 69719497 D1 DE69719497 D1 DE 69719497D1 DE 69719497 T DE69719497 T DE 69719497T DE 69719497 T DE69719497 T DE 69719497T DE 69719497 D1 DE69719497 D1 DE 69719497D1
Authority
DE
Germany
Prior art keywords
protein
containing solution
purification
cellulase
crystalline form
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69719497T
Other languages
English (en)
Other versions
DE69719497T2 (de
Inventor
Anders Rancke-Madsen
Aage Laustsen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novozymes AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes AS filed Critical Novozymes AS
Publication of DE69719497D1 publication Critical patent/DE69719497D1/de
Application granted granted Critical
Publication of DE69719497T2 publication Critical patent/DE69719497T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C07K1/306—Extraction; Separation; Purification by precipitation by crystallization
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification
    • Y10S435/816—Enzyme separation or purification by solubility

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
DE69719497T 1996-03-15 1997-03-14 Verfahren zur proteinaufreinigung aus einer proteinhaltigen lösung Expired - Lifetime DE69719497T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK30596 1996-03-15
PCT/DK1997/000113 WO1997034919A1 (en) 1996-03-15 1997-03-14 Method for purification of a protein from a protein containing solution

Publications (2)

Publication Number Publication Date
DE69719497D1 true DE69719497D1 (de) 2003-04-10
DE69719497T2 DE69719497T2 (de) 2003-10-30

Family

ID=8092063

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69719497T Expired - Lifetime DE69719497T2 (de) 1996-03-15 1997-03-14 Verfahren zur proteinaufreinigung aus einer proteinhaltigen lösung

Country Status (10)

Country Link
US (1) US6087148A (de)
EP (1) EP0894091B1 (de)
JP (1) JP2000506534A (de)
CN (1) CN1216899C (de)
AT (1) ATE233784T1 (de)
AU (1) AU2151997A (de)
DE (1) DE69719497T2 (de)
DK (1) DK0894091T3 (de)
IN (1) IN190120B (de)
WO (1) WO1997034919A1 (de)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997034919A1 (en) * 1996-03-15 1997-09-25 Novo Nordisk A/S Method for purification of a protein from a protein containing solution
SG121739A1 (en) * 1998-04-27 2006-05-26 Altus Pharmaceuticals Inc Stabilized protein crystals, formulations containing them and methods of making them
DE19859876A1 (de) * 1998-12-23 2000-06-29 Fraunhofer Ges Forschung Verfahren zur Kristallisation aus Lösungen
GB9910975D0 (en) 1999-05-13 1999-07-14 Univ Strathclyde Rapid dehydration of proteins
US6663602B2 (en) 2000-06-16 2003-12-16 Novo Nordisk A/S Injection device
DE60231534D1 (de) 2001-10-25 2009-04-23 Kyowa Hakko Kogyo Kk Kristall von oxidiertem glutathion und verfahren zu dessen herstellung
US7674885B2 (en) * 2002-11-01 2010-03-09 Bayer Healthcare Llc Process for concentration of macromolecules
CA2584760C (en) 2004-10-21 2013-12-24 Novo Nordisk A/S Dial-down mechanism for wind-up pen
US8088900B2 (en) 2006-03-30 2012-01-03 Novo Nordisk A/S Two-phase precipitation of proteins
WO2007132019A1 (en) 2006-05-16 2007-11-22 Novo Nordisk A/S A gearing mechanism for an injection device
WO2007134954A1 (en) 2006-05-18 2007-11-29 Novo Nordisk A/S An injection device with mode locking means
DE102008029401A1 (de) * 2008-06-23 2009-12-24 Bayer Technology Services Gmbh Verfahren zur Kristallisation von Peptiden und Proteinen
FR2943686B1 (fr) * 2009-03-30 2013-11-01 Roquette Freres Procede d'obtention d'une preparation de beta-amylases a partir des fractions solubles de plantes amidonnieres
BR112014016148A8 (pt) 2011-12-29 2017-07-04 Novo Nordisk As mecanismo de aumento/redução para dispositivo de injeção automático e dispositivo de injeção automático à base de mola de torção
WO2023200284A1 (ko) * 2022-04-15 2023-10-19 재단법인대구경북과학기술원 생체 시료 내 지질 연관 단백질의 질량 분석을 위한 지질 연관 단백질의 정제 및 농축 방법
WO2024032881A1 (en) * 2022-08-10 2024-02-15 Arla Foods Amba Crystallisation of a protein capable of crystallizing under salting-in conditions using multiple protein feeds

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3664926A (en) * 1970-02-26 1972-05-23 Merck & Co Inc Crystallization of l-asparaginase
DE4001238A1 (de) * 1990-01-18 1991-07-25 Basf Ag Verfahren zur anreicherung bzw. reinigung von biomolekuelen
DK115890D0 (da) * 1990-05-09 1990-05-09 Novo Nordisk As Enzym
DK83093D0 (da) * 1993-07-09 1993-07-09 Novo Nordisk As Fremgangsmaade
WO1997034919A1 (en) * 1996-03-15 1997-09-25 Novo Nordisk A/S Method for purification of a protein from a protein containing solution

Also Published As

Publication number Publication date
WO1997034919A1 (en) 1997-09-25
CN1216899C (zh) 2005-08-31
EP0894091B1 (de) 2003-03-05
ATE233784T1 (de) 2003-03-15
DK0894091T3 (da) 2003-06-10
JP2000506534A (ja) 2000-05-30
IN190120B (de) 2003-06-14
CN1213377A (zh) 1999-04-07
EP0894091A1 (de) 1999-02-03
AU2151997A (en) 1997-10-10
US6087148A (en) 2000-07-11
DE69719497T2 (de) 2003-10-30

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