ATE242780T1 - Verfahren zur isolierung von nukleinsäure und vorrichtung hierfür - Google Patents

Verfahren zur isolierung von nukleinsäure und vorrichtung hierfür

Info

Publication number
ATE242780T1
ATE242780T1 AT99947774T AT99947774T ATE242780T1 AT E242780 T1 ATE242780 T1 AT E242780T1 AT 99947774 T AT99947774 T AT 99947774T AT 99947774 T AT99947774 T AT 99947774T AT E242780 T1 ATE242780 T1 AT E242780T1
Authority
AT
Austria
Prior art keywords
nucleic acid
filter
retentate
retained
isolation
Prior art date
Application number
AT99947774T
Other languages
English (en)
Inventor
Andrew Martin Mitchell
Neil James Butt
Christopher Peter Jones
Original Assignee
Whatman Bioscience Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9822141.9A external-priority patent/GB9822141D0/en
Application filed by Whatman Bioscience Ltd filed Critical Whatman Bioscience Ltd
Application granted granted Critical
Publication of ATE242780T1 publication Critical patent/ATE242780T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT99947774T 1998-10-09 1999-10-08 Verfahren zur isolierung von nukleinsäure und vorrichtung hierfür ATE242780T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB9822141.9A GB9822141D0 (en) 1998-10-09 1998-10-09 Isolation method and apparatus
GB9918193A GB2346370B (en) 1998-10-09 1999-08-02 Nucleic acid isolation method and apparatus
PCT/GB1999/003357 WO2000021973A1 (en) 1998-10-09 1999-10-08 Isolation method for nucleic acid and apparatus

Publications (1)

Publication Number Publication Date
ATE242780T1 true ATE242780T1 (de) 2003-06-15

Family

ID=26314493

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99947774T ATE242780T1 (de) 1998-10-09 1999-10-08 Verfahren zur isolierung von nukleinsäure und vorrichtung hierfür

Country Status (6)

Country Link
US (2) US20020010323A1 (de)
EP (1) EP1119576B1 (de)
AT (1) ATE242780T1 (de)
AU (1) AU6114199A (de)
DE (1) DE69908795T2 (de)
WO (1) WO2000021973A1 (de)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6844158B1 (en) * 1997-12-22 2005-01-18 Hitachi Chemical Co., Ltd. Direct RT-PCR on oligonucleotide-immobilized PCR microplates
US6958392B2 (en) * 1998-10-09 2005-10-25 Whatman, Inc. Methods for the isolation of nucleic acids and for quantitative DNA extraction and detection for leukocyte evaluation in blood products
WO2001077316A2 (en) * 2000-04-11 2001-10-18 Bureco Ag Method of nucleic acid recovery
GB2363381B (en) 2000-06-12 2004-11-24 Cambridge Molecular Tech Solution
WO2002014560A1 (en) * 2000-08-16 2002-02-21 Whatman, Inc. Transfusion medicine leukodepletion filter devices as a source of genetic material for genotyping studies
US6638428B2 (en) * 2000-10-31 2003-10-28 Hitachi Chemical Research Center, Inc. Method of preventing formation of bubbles during filtration operations
JP5416326B2 (ja) * 2000-10-31 2014-02-12 ヒタチ ケミカル リサーチ センター インコーポレイテッド 核ｍＲＮＡの収集および使用方法
US20030003600A1 (en) * 2001-07-02 2003-01-02 Fuji Photo Film Co., Ltd. Biochemical analysis kit and method for exposing stimulable phosphor sheet
KR100764966B1 (ko) * 2001-07-09 2007-10-08 아사히 가세이 가부시키가이샤 부직포를 이용한 핵산 정제법 및 검출법
GB0129662D0 (en) 2001-12-11 2002-01-30 Whatman Internat Ltd Stabilisation of double-stranded nucleic acids
FR2838066B1 (fr) * 2002-04-09 2004-06-25 Genesystems Procede et automate d'extraction d'acides nucleiques a partir d'un melange complexe
EP1504121A4 (de) * 2002-04-24 2005-06-29 Hitachi Chemical Res Ct Inc Vorrichtung und verfahren zur quantifizierung von mrna aus vollblut mit hohem durchsatz
US7745180B2 (en) 2002-04-24 2010-06-29 Hitachi Chemical Co., Ltd. Device and method for high-throughput quantification of mRNA from whole blood
US20060094015A1 (en) * 2002-10-04 2006-05-04 Whatman Inc. Methods and materials for using chemical compounds as a tool for nucleic acid storage on media of nucleic acid purification systems
US20050227225A1 (en) * 2004-04-07 2005-10-13 Roche Molecular Systems, Inc. Stabilization of biomolecules in samples
DE102005047736B4 (de) * 2005-09-29 2008-08-14 Aj Innuscreen Gmbh Verfahren und System zur Isolierung von Nukleinsäuren aus beliebigen komplexen Ausgangsmaterialien
US10125388B2 (en) 2007-10-31 2018-11-13 Akonni Biosystems, Inc. Integrated sample processing system
US20090111193A1 (en) 2007-10-31 2009-04-30 Cooney Christopher G Sample preparation device
US7759112B2 (en) * 2007-10-31 2010-07-20 Akonni Biosystems, Inc. Apparatus, system, and method for purifying nucleic acids
US9428746B2 (en) 2007-10-31 2016-08-30 Akonni Biosystems, Inc. Method and kit for purifying nucleic acids
CN103173432B (zh) 2011-12-22 2020-08-04 通用电气公司 分离核酸的方法及装置
KR101915675B1 (ko) 2012-02-07 2018-11-06 주식회사 미코바이오메드 초고속 핵산 추출 장치, 및 이를 이용하는 핵산 추출 방법
WO2014151177A1 (en) 2013-03-15 2014-09-25 3M Innovative Properties Company Sample concentrator and method of use
US11214823B2 (en) 2015-12-22 2022-01-04 Canon U.S.A., Inc. Sample-to-answer system for microorganism detection featuring target enrichment, amplification and detection
CN105624152B (zh) * 2016-03-01 2019-02-19 中国人民解放军第二军医大学 一种用于核酸扩增的酵母样真菌总dna无仪器提取方法
GB201703383D0 (en) 2017-03-02 2017-04-19 Gargle Tech Ltd Testing for particulates
WO2018202864A1 (fr) 2017-05-05 2018-11-08 bioMérieux Procédé de détection d'une réponse cellulaire immune
KR102114148B1 (ko) * 2018-01-03 2020-05-22 재단법인 구미전자정보기술원 식품으로부터 미생물을 검출하는 방법
JP7454264B2 (ja) 2018-09-05 2024-03-22 ヒーロー サイエンティフィック リミテッド 粒子検査
WO2021181338A1 (en) * 2020-03-11 2021-09-16 Hero Scientific Ltd. Testing for viruses and cellular biomarkers
CA3202405A1 (en) 2021-01-06 2022-07-14 Zvi Feldman Filtration sampling devices
KR102412078B1 (ko) 2021-12-23 2022-06-22 홍승범 다공성 이온 전하 결정체를 이용한 핵산 추출방법

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3639949A1 (de) * 1986-11-22 1988-06-09 Diagen Inst Molekularbio Verfahren zur trennung von langkettigen nukleinsaeuren
DE4034036C2 (de) * 1990-10-26 1994-03-03 Diagen Inst Molekularbio Vorrichtung und Verfahren zur Isolierung von Nukleinsäuren aus Zellsuspensionen
US5652141A (en) * 1990-10-26 1997-07-29 Oiagen Gmbh Device and process for isolating nucleic acids from cell suspension
US5187083A (en) * 1990-11-13 1993-02-16 Specialty Laboratories, Inc. Rapid purification of DNA
US5234824A (en) * 1990-11-13 1993-08-10 Specialty Laboratories, Inc. Rapid purification of DNA
EP0814156B1 (de) * 1996-06-18 2003-03-05 The Institute Of Physical & Chemical Research Verfahren zur Reinigung von DNS
WO1998011989A1 (en) * 1996-09-18 1998-03-26 Promega Corporation Improved filtration system and method

Also Published As

Publication number Publication date
DE69908795T2 (de) 2004-04-22
AU6114199A (en) 2000-05-01
US20040054163A1 (en) 2004-03-18
US20020010323A1 (en) 2002-01-24
EP1119576A1 (de) 2001-08-01
EP1119576B1 (de) 2003-06-11
DE69908795D1 (de) 2003-07-17
WO2000021973A1 (en) 2000-04-20

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