DE2246695C3 - Verfahren zur Herstellung von Cholesterinoxidase und Verwendung derselben zur Bestimmung von Cholesterin - Google Patents

Verfahren zur Herstellung von Cholesterinoxidase und Verwendung derselben zur Bestimmung von Cholesterin

Info

Publication number
DE2246695C3
DE2246695C3 DE2246695A DE2246695A DE2246695C3 DE 2246695 C3 DE2246695 C3 DE 2246695C3 DE 2246695 A DE2246695 A DE 2246695A DE 2246695 A DE2246695 A DE 2246695A DE 2246695 C3 DE2246695 C3 DE 2246695C3
Authority
DE
Germany
Prior art keywords
cholesterol
cholesterol oxidase
activity
oxidase
enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DE2246695A
Other languages
German (de)
English (en)
Other versions
DE2246695A1 (de
DE2246695B2 (de
Inventor
William Harrow Middlesex Richmond
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Research Development Corp UK
Original Assignee
National Research Development Corp UK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National Research Development Corp UK filed Critical National Research Development Corp UK
Publication of DE2246695A1 publication Critical patent/DE2246695A1/de
Publication of DE2246695B2 publication Critical patent/DE2246695B2/de
Application granted granted Critical
Publication of DE2246695C3 publication Critical patent/DE2246695C3/de
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/60—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving cholesterol
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S210/00—Liquid purification or separation
    • Y10S210/918—Miscellaneous specific techniques
    • Y10S210/922—Oil spill cleanup, e.g. bacterial
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification
    • Y10S435/815—Enzyme separation or purification by sorption
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/863—Mycobacterium
    • Y10S435/866—Mycobacterium smegmatis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/872—Nocardia
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/913—Aspergillus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/921—Candida
    • Y10S435/923—Candida lipolytica
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/921—Candida
    • Y10S435/924—Candida tropicalis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/94—Saccharomyces
    • Y10S435/942—Saccharomyces cerevisiae

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DE2246695A 1971-09-22 1972-09-22 Verfahren zur Herstellung von Cholesterinoxidase und Verwendung derselben zur Bestimmung von Cholesterin Expired DE2246695C3 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB4409571 1971-09-22
GB2865072*[A GB1385319A (en) 1971-09-22 1972-06-19 Enzyme preparations
US289581A US3907642A (en) 1971-09-22 1972-09-15 Cholesterol oxidase and method of extracting from nocardia
US435892A US3907645A (en) 1971-09-22 1974-01-23 Cholesterol assay

Publications (3)

Publication Number Publication Date
DE2246695A1 DE2246695A1 (de) 1973-03-29
DE2246695B2 DE2246695B2 (de) 1980-11-06
DE2246695C3 true DE2246695C3 (de) 1981-08-20

Family

ID=27448749

Family Applications (3)

Application Number Title Priority Date Filing Date
DE2246695A Expired DE2246695C3 (de) 1971-09-22 1972-09-22 Verfahren zur Herstellung von Cholesterinoxidase und Verwendung derselben zur Bestimmung von Cholesterin
DE2265122A Expired DE2265122C2 (de) 1971-09-22 1972-09-22 Verfahren zur Bestimmung von Cholesterin
DE2265121A Expired DE2265121C3 (de) 1971-09-22 1972-09-22 Enzympräparat zur Bestimmung von Cholesterin und Verfahren zu seiner Herstellung

Family Applications After (2)

Application Number Title Priority Date Filing Date
DE2265122A Expired DE2265122C2 (de) 1971-09-22 1972-09-22 Verfahren zur Bestimmung von Cholesterin
DE2265121A Expired DE2265121C3 (de) 1971-09-22 1972-09-22 Enzympräparat zur Bestimmung von Cholesterin und Verfahren zu seiner Herstellung

Country Status (6)

Country Link
US (3) US3907642A (enExample)
CH (2) CH596553A5 (enExample)
DE (3) DE2246695C3 (enExample)
FR (1) FR2187125A5 (enExample)
GB (1) GB1385319A (enExample)
NL (1) NL168268C (enExample)

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GB1385319A (en) * 1971-09-22 1975-02-26 Nat Res Dev Enzyme preparations
DE2224131B2 (de) * 1972-05-17 1974-06-27 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren zur Gewinnung des Enzyms Cholesterinoxydase, welches Cholesterin mit O tief 2 zu Cholestenon und H tief 2 O tief 2 oxydiert
DE2264847C2 (de) * 1972-05-17 1978-11-30 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren und Reagenz zur Bestimmung von Cholesterin
US4045296A (en) * 1972-08-09 1977-08-30 Beckman Instruments, Inc. Rate sensing batch analysis method and enzyme used therein
US4164448A (en) * 1973-12-07 1979-08-14 Boehringer Mannheim Gmbh Activation of cholesterol oxidase for cholesterol assay
US3884764A (en) * 1974-03-25 1975-05-20 Eastman Kodak Co Method and composition for blood serum cholesterol analysis
CA1056282A (en) * 1974-03-25 1979-06-12 Charles T. Goodhue Multilayer analytical elements for use in the assay of cholesterol
US3979283A (en) * 1974-09-25 1976-09-07 Bioteknika International, Inc. Microbial degradation of DDT
DE2456586C3 (de) * 1974-11-29 1979-09-27 Boehringer Mannheim Gmbh, 6800 Mannheim Verwendung von bestimmten Mikroorganismenstämmen zur Gewinnung von Cholesterinoxidase, welche Cholesterin mit O2 zu Cholestenon + H2 O2 oxidiert
JPS552277B2 (enExample) * 1974-12-27 1980-01-19
JPS527484A (en) * 1975-07-04 1977-01-20 Kikkoman Corp Process for preparing cholesterol oxidase
US4035237A (en) * 1975-11-07 1977-07-12 Eastman Kodak Company Method for the preparation of cholesterol oxidase
CA1067844A (en) * 1975-12-11 1979-12-11 Eastman Kodak Company Method and medium for producing cholesterol oxidase
US4052263A (en) * 1975-12-11 1977-10-04 Eastman Kodak Company Production of cholesterol esterase using Nocardia cholesterolicum
US4040908A (en) * 1976-03-12 1977-08-09 Children's Hospital Medical Center Polarographic analysis of cholesterol and other macromolecular substances
US4184921A (en) * 1976-03-25 1980-01-22 Boehringer Mannheim Gmbh Process and reagent for determining cholesterol
US4043870A (en) * 1976-05-11 1977-08-23 The Upjohn Company Process of purifying cholesterol oxidase
US4066083A (en) * 1976-06-03 1978-01-03 Pentapharm A.G. Sterile surgical collagen product
US4161425A (en) * 1976-07-01 1979-07-17 Beckman Instruments, Inc. Enzymatic reagent system for total cholesterol assay using oxygen-rate method
DE2649749A1 (de) * 1976-10-29 1978-05-11 Boehringer Mannheim Gmbh Verfahren und reagens zur bestimmung von gesamtcholesterin oder gebundenem cholesterin
USRE32016E (en) * 1976-12-10 1985-10-29 Eastman Kodak Company Analysis of lactic acid or lactate using lactate oxidase
US4166763A (en) * 1976-12-10 1979-09-04 Eastman Kodak Company Analysis of lactic acid or lactate using lactate oxidase
US4275151A (en) * 1977-02-03 1981-06-23 Eastman Kodak Company Hydrolysis of protein-bound cholesterol esters
US4250255A (en) * 1977-07-11 1981-02-10 Eastman Kodak Company Assay method for isoenzyme activity
US4414326A (en) * 1978-04-24 1983-11-08 Goldberg Jack M Diagnostic agents
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US4215993A (en) * 1978-12-04 1980-08-05 Data Medical Associates, Inc. Precipitating reagent and method for isolation and determination of high density lipoproteins in human serum
US4247631A (en) * 1979-01-31 1981-01-27 Millipore Corporation Reagent and method for the analytic determination of hydrogen peroxide
US4378429A (en) * 1979-08-23 1983-03-29 Modrovich Ivan Endre Enzymatic method and stabilized solutions for determining total cholesterol in human serum
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DE3165392D1 (en) * 1980-07-10 1984-09-13 Ivan Endre Modrovich Stabilized enzymatic solutions and method for determining total cholesterol in human serum
US4374930A (en) * 1981-10-19 1983-02-22 Eastman Kodak Company Method for the purification of cholesterol oxidase
US5047327A (en) * 1982-04-02 1991-09-10 Ivan E. Modrovich Time-stable liquid cholesterol assay compositions
US5062958A (en) * 1988-05-12 1991-11-05 Biotrol, Inc. Method for water purification
US5156954A (en) * 1989-03-08 1992-10-20 Cholestech Corporation Assay device and method using a signal-modulating compound
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US5487978A (en) * 1988-11-03 1996-01-30 Gds Technology, Inc. Method, composition and device for the determination of cholesterol using cholesterol oxidase obtained from bacterial strain NRRL B-18713
CA2002890C (en) * 1988-11-14 1999-12-14 Susan Long Expression of dna sequences derived from nocardioform microorganisms
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US4948297A (en) * 1989-07-24 1990-08-14 Fleming Joseph W Correction method for contaminated sites
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US5876671A (en) * 1993-03-24 1999-03-02 The Regents Of The University Of California Office Of Technology Transfer Sonication standard laboratory module
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US6057147A (en) * 1997-01-21 2000-05-02 Overland; Bert A. Apparatus and method for bioremediation of hydrocarbon-contaminated objects
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US9079786B2 (en) * 2006-06-20 2015-07-14 Johannes van Leeuwen Purification of thin stillage from dry-grind corn milling with fungi
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2265121C3 (de) * 1971-09-22 1979-07-26 National Research Development Corp., London Enzympräparat zur Bestimmung von Cholesterin und Verfahren zu seiner Herstellung

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Patent Citations (1)

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Non-Patent Citations (1)

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Title
In Betracht gezogene ältere Patente: DE-PS 22 65 121

Also Published As

Publication number Publication date
FR2187125A5 (enExample) 1974-01-11
NL168268B (nl) 1981-10-16
DE2265122C2 (de) 1982-08-19
NL168268C (nl) 1985-01-16
NL7212811A (enExample) 1973-03-26
US3907642A (en) 1975-09-23
US3899376A (en) 1975-08-12
CH588077A5 (enExample) 1977-05-31
GB1385319A (en) 1975-02-26
DE2265121C3 (de) 1979-07-26
US3907645A (en) 1975-09-23
DE2265121A1 (de) 1976-05-06
DE2265121B2 (de) 1978-11-02
DE2265122A1 (de) 1976-05-06
DE2246695A1 (de) 1973-03-29
CH596553A5 (enExample) 1978-03-15
DE2246695B2 (de) 1980-11-06

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