DD298426A5 - Verfahren zur herstellung von neuen plasmiden und deren verwendung bei der gewinnung eines plasminogenaktivators - Google Patents

Verfahren zur herstellung von neuen plasmiden und deren verwendung bei der gewinnung eines plasminogenaktivators Download PDF

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Publication number
DD298426A5
DD298426A5 DD90342894A DD34289490A DD298426A5 DD 298426 A5 DD298426 A5 DD 298426A5 DD 90342894 A DD90342894 A DD 90342894A DD 34289490 A DD34289490 A DD 34289490A DD 298426 A5 DD298426 A5 DD 298426A5
Authority
DD
German Democratic Republic
Prior art keywords
plasmid
coli
rscu
plasmids
structural gene
Prior art date
Application number
DD90342894A
Other languages
German (de)
English (en)
Inventor
Regina E Brigelius-Flohe
Leopold Flohe
Wolfgang Hillen
Gerd Steffens
Wolfgang Strassburger
Martin R F Wilhelm
Original Assignee
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Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by ������@������������k�� filed Critical ������@������������k��
Publication of DD298426A5 publication Critical patent/DD298426A5/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6462—Plasminogen activators u-Plasminogen activator (3.4.21.73), i.e. urokinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
DD90342894A 1989-07-19 1990-07-18 Verfahren zur herstellung von neuen plasmiden und deren verwendung bei der gewinnung eines plasminogenaktivators DD298426A5 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE3923866 1989-07-19

Publications (1)

Publication Number Publication Date
DD298426A5 true DD298426A5 (de) 1992-02-20

Family

ID=6385369

Family Applications (1)

Application Number Title Priority Date Filing Date
DD90342894A DD298426A5 (de) 1989-07-19 1990-07-18 Verfahren zur herstellung von neuen plasmiden und deren verwendung bei der gewinnung eines plasminogenaktivators

Country Status (30)

Country Link
US (1) US5637503A (el)
EP (1) EP0408945B1 (el)
JP (1) JP3236284B2 (el)
KR (1) KR0167761B1 (el)
AT (1) ATE131534T1 (el)
AU (1) AU631662B2 (el)
CA (1) CA2020656C (el)
DD (1) DD298426A5 (el)
DE (2) DE4020438A1 (el)
DK (1) DK0408945T3 (el)
ES (1) ES2083399T3 (el)
FI (1) FI102386B (el)
GR (1) GR3019294T3 (el)
HK (1) HK1005193A1 (el)
HR (1) HRP920596B1 (el)
HU (1) HU214243B (el)
IE (1) IE901849A1 (el)
IL (1) IL94529A (el)
LT (1) LT3611B (el)
LV (1) LV10120B (el)
MT (1) MTP1063B (el)
NO (1) NO304952B1 (el)
NZ (1) NZ234542A (el)
PL (1) PL166199B1 (el)
PT (1) PT94776B (el)
RU (1) RU2073720C1 (el)
SI (1) SI9011347B (el)
SK (1) SK306790A3 (el)
YU (1) YU48371B (el)
ZA (1) ZA904006B (el)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3777528A (en) * 1969-11-27 1973-12-11 Cefilac Method of hot piercing metal billets

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4101736A1 (de) * 1991-01-22 1992-07-23 Gruenenthal Gmbh Neue als plasminogenaktivatoren einsetzbare polypeptide, dafuer codierende plasmide und verfahren zu deren herstellung und deren verwendung
DE4440892A1 (de) * 1994-11-17 1996-05-23 Gruenenthal Gmbh Proteine mit fibrinolytischen und gerinnungshemmenden Eigenschaften
DE4442665A1 (de) * 1994-11-30 1996-06-05 Gruenenthal Gmbh Chimäre Proteine mit fibrinolytischen und thrombinhemmenden Eigenschaften
KR100467034B1 (ko) * 1996-12-27 2006-04-07 주식회사 엘지생활건강 압축성형타입오일케익화장료및그의제조방법
KR100372233B1 (ko) * 1997-03-12 2003-06-11 주식회사 코리아나화장품 미백 파우더, 그의 제조방법 및 그를 함유하는 메이크업 미백 화장료
KR100341638B1 (ko) * 2000-03-17 2002-06-22 유상옥,송운한 기능성 표면처리 분체의 제조 방법 및 이를 함유하는메이크업 화장료 조성물
JP4124639B2 (ja) 2002-12-17 2008-07-23 株式会社日本触媒 大腸菌を用いたｓ−ヒドロキシニトリルリアーゼの製造方法
US7906276B2 (en) * 2004-06-30 2011-03-15 Kimberly-Clark Worldwide, Inc. Enzymatic detection techniques
EP2147105B1 (en) 2007-05-02 2013-04-24 Merial Limited Dna plasmids having improved expression and stability
RU2553533C2 (ru) * 2013-10-23 2015-06-20 Общество с ограниченной ответственностью "Международный биотехнологический центр "Генериум" ("МБЦ "Генериум") Способ выделения и очистки рекомбинантной человеческой проурокиназы м5
CN104555732A (zh) * 2015-01-18 2015-04-29 河南省中原起重机械总厂 一种多功能门式起重机

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4558010A (en) * 1980-04-03 1985-12-10 Abbott Laboratories Recombinant deoxyribonucleic acid which codes for plasminogen activator and method of making plasminogen activator protein therefrom
US4370417A (en) * 1980-04-03 1983-01-25 Abbott Laboratories Recombinant deoxyribonucleic acid which codes for plasminogen activator
FI88932C (fi) * 1982-04-15 1993-07-26 Genentech Inc Framstaellning av funktionellt maenskligt urokinasprotein
US4710464A (en) * 1984-09-27 1987-12-01 Eli Lilly And Company Transcription terminators
WO1986004351A1 (en) * 1985-01-25 1986-07-31 Sagami Chemical Research Center Stabilized human prourokinase
FR2594845B1 (fr) * 1986-02-21 1989-12-01 Genetica Preparation par voie microbiologique de l'activateur tissulaire humain du plasminogene (t-pa) et conversion de l'enzyme ainsi obtenue en sa forme active
FI100106B (fi) * 1986-12-05 1997-09-30 Novartis Ag Menetelmä plasminogeenin yksisäikeisen yhdistelmäaktivaattorin valmist amiseksi
EP0303028A1 (en) * 1987-08-10 1989-02-15 GRUPPO LEPETIT S.p.A. Method for preparing single chain urokinase
JP2970811B2 (ja) * 1987-10-30 1999-11-02 オンコゲン ア リミテッド パートナーシップ 原核生物細胞中にポリペプチドを調製するための発現システム

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3777528A (en) * 1969-11-27 1973-12-11 Cefilac Method of hot piercing metal billets

Also Published As

Publication number Publication date
AU5883290A (en) 1991-01-24
SK280028B6 (sk) 1999-07-12
LV10120A (lv) 1994-05-10
PT94776A (pt) 1991-03-20
SI9011347A (sl) 1998-04-30
LTIP776A (en) 1995-01-31
ZA904006B (en) 1991-03-27
YU48371B (sh) 1998-07-10
FI102386B1 (fi) 1998-11-30
RU2073720C1 (ru) 1997-02-20
HRP920596B1 (en) 1999-12-31
KR910003105A (ko) 1991-02-26
PL286087A1 (en) 1991-03-11
US5637503A (en) 1997-06-10
DE4020438A1 (de) 1991-01-31
JP3236284B2 (ja) 2001-12-10
EP0408945A1 (de) 1991-01-23
PL166199B1 (pl) 1995-04-28
SK306790A3 (en) 1999-07-12
SI9011347B (sl) 1999-02-28
ATE131534T1 (de) 1995-12-15
CA2020656A1 (en) 1991-01-20
DE59009961D1 (de) 1996-01-25
PT94776B (pt) 1997-09-30
IL94529A (en) 1997-11-20
LT3611B (en) 1995-12-27
NO902720D0 (no) 1990-06-19
FI102386B (fi) 1998-11-30
IL94529A0 (en) 1991-03-10
NZ234542A (en) 1991-09-25
HRP920596A2 (en) 1998-06-30
HU903970D0 (en) 1990-11-28
FI903640A0 (fi) 1990-07-18
DK0408945T3 (da) 1996-04-09
LV10120B (en) 1994-10-20
HUT54211A (en) 1991-01-28
EP0408945B1 (de) 1995-12-13
JPH0365189A (ja) 1991-03-20
HU214243B (hu) 1998-03-02
GR3019294T3 (en) 1996-06-30
CA2020656C (en) 2001-05-22
NO304952B1 (no) 1999-03-08
MTP1063B (en) 1991-04-08
ES2083399T3 (es) 1996-04-16
HK1005193A1 (en) 1998-12-24
YU134790A (sh) 1992-12-21
AU631662B2 (en) 1992-12-03
NO902720L (no) 1991-01-21
KR0167761B1 (ko) 1999-01-15
IE901849A1 (en) 1991-06-19

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ENJ Ceased due to non-payment of renewal fee