DE3877197T2 - Verfahren zur herstellung von hirudin. - Google Patents

Verfahren zur herstellung von hirudin.

Info

Publication number
DE3877197T2
DE3877197T2 DE19883877197 DE3877197T DE3877197T2 DE 3877197 T2 DE3877197 T2 DE 3877197T2 DE 19883877197 DE19883877197 DE 19883877197 DE 3877197 T DE3877197 T DE 3877197T DE 3877197 T2 DE3877197 T2 DE 3877197T2
Authority
DE
Germany
Prior art keywords
hirudine
producing
producing hirudine
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE19883877197
Other languages
English (en)
Other versions
DE3877197D1 (de
Inventor
Yoshio Furutani
Masaru Honjo
Akira Nakayama
Kouichi Kawamura
Izumi Mita
Kazunori Ando
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Institute of Advanced Industrial Science and Technology AIST
Original Assignee
Agency of Industrial Science and Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agency of Industrial Science and Technology filed Critical Agency of Industrial Science and Technology
Application granted granted Critical
Publication of DE3877197D1 publication Critical patent/DE3877197D1/de
Publication of DE3877197T2 publication Critical patent/DE3877197T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/33—Fusion polypeptide fusions for targeting to specific cell types, e.g. tissue specific targeting, targeting of a bacterial subspecies

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE19883877197 1988-07-26 1988-08-30 Verfahren zur herstellung von hirudin. Expired - Fee Related DE3877197T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP63184557A JPH0648988B2 (ja) 1988-07-26 1988-07-26 トロンビン阻害物質の製造法

Publications (2)

Publication Number Publication Date
DE3877197D1 DE3877197D1 (de) 1993-02-11
DE3877197T2 true DE3877197T2 (de) 1993-04-29

Family

ID=16155291

Family Applications (1)

Application Number Title Priority Date Filing Date
DE19883877197 Expired - Fee Related DE3877197T2 (de) 1988-07-26 1988-08-30 Verfahren zur herstellung von hirudin.

Country Status (3)

Country Link
EP (1) EP0352387B1 (de)
JP (1) JPH0648988B2 (de)
DE (1) DE3877197T2 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4009268A1 (de) * 1990-03-22 1991-09-26 Consortium Elektrochem Ind Sekretion von hirudinderivaten
JPH0751066B2 (ja) * 1990-09-14 1995-06-05 重三 鵜高 プロテアーゼインヒビター遺伝子及び該遺伝子を保有する微生物並びに該微生物を用いるプロテアーゼインヒビターの製造法
CA2072375C (en) * 1990-11-08 2000-01-11 Satoru Misawa Hirudin analog, method of manufacturing thereof and anti-coagulant composition, and secretion vector, transformed microorganisms containing saidvector and manufacturing method of products which is produced from said microorganism
EP0929577B1 (de) 1996-09-24 2003-06-04 The Procter & Gamble Company Stabilisierte proteine mit protease-inhibitorfunktion und varianten davon

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0669377B2 (ja) * 1985-11-25 1994-09-07 工業技術院長 Ｄｎａ塩基配列およびベクタ−

Also Published As

Publication number Publication date
EP0352387A1 (de) 1990-01-31
JPH0648988B2 (ja) 1994-06-29
EP0352387B1 (de) 1992-12-30
JPH0235084A (ja) 1990-02-05
DE3877197D1 (de) 1993-02-11

Similar Documents

Publication Publication Date Title
DE3866906D1 (de) Verfahren zur herstellung von polyolefinen.
DE3865476D1 (de) Verfahren zur herstellung von aminopropylalkoxysilanen.
DE3853160T2 (de) Verfahren zur herstellung von elektrophotographien.
DE68908193D1 (de) Verfahren zur herstellung von fluorglimmer.
DE3860666D1 (de) Verfahren zur herstellung von hexabromcyclododekan.
DE3861891D1 (de) Verfahren zur herstellung von epichlorhydrinen.
DE68901908T2 (de) Verfahren zur herstellung von mesophasepech.
DE68918601D1 (de) Verfahren zur herstellung von ortho-nitrobenzonitrilen.
DE3863586D1 (de) Verfahren zur herstellung von m-triflourmethylphenylacetonitril.
DE58902073D1 (de) Verfahren zur herstellung von organohalogensilanen.
DE68904436D1 (de) Verfahren zur herstellung von 1-alkyl-5-nitro-imidazolen.
DE3884577D1 (de) Verfahren zur herstellung von polyolefinen.
DE3863321D1 (de) Verfahren zur herstellung von naphthacenen.
DE68905908D1 (de) Verfahren zur herstellung von alkansulfonamiden.
DE68906638D1 (de) Verfahren zur herstellung von 4-methylpyrazolen.
DE58901624D1 (de) Verfahren zur herstellung von fluorbenzolen.
DE3877197T2 (de) Verfahren zur herstellung von hirudin.
DE58902083D1 (de) Verfahren zur herstellung von dihydroxydionen.
DE68903517T2 (de) Verfahren zur herstellung von acylisocyanaten.
DE68907097D1 (de) Verfahren zur herstellung von ketodiestern.
DE68903818D1 (de) Verfahren zur herstellung von tetranatriumpyrophosphat.
DE68904729D1 (de) Verfahren zur herstellung von alpha-olephinen.
DE68904748D1 (de) Verfahren zur herstellung von p-isobutylstyrol.
DE68921984D1 (de) Verfahren zur herstellung von 4-sulfo-2-chlorbenzoesäure.
DE58901012D1 (de) Verfahren zur herstellung von formylcyclopropan.

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee