ES2083399T3 - Plasmidos, su procedimiento de preparacion y su uso en la obtencion de un activador de plasminogeno. - Google Patents

Plasmidos, su procedimiento de preparacion y su uso en la obtencion de un activador de plasminogeno.

Info

Publication number
ES2083399T3
ES2083399T3 ES90112400T ES90112400T ES2083399T3 ES 2083399 T3 ES2083399 T3 ES 2083399T3 ES 90112400 T ES90112400 T ES 90112400T ES 90112400 T ES90112400 T ES 90112400T ES 2083399 T3 ES2083399 T3 ES 2083399T3
Authority
ES
Spain
Prior art keywords
plasmids
rscu
protein
plasminogen activator
synthetic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90112400T
Other languages
English (en)
Inventor
Regina E Dr Brigelius-Flohe
Leopold Prof Dr Flohe
Wolfgang Prof Dr Hillen
Gerd J Prof Dr Steffens
Wolfgang Dr Strassburger
Martin R F Dr Wilhelm
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gruenenthal GmbH
Original Assignee
Gruenenthal GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gruenenthal GmbH filed Critical Gruenenthal GmbH
Application granted granted Critical
Publication of ES2083399T3 publication Critical patent/ES2083399T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6462—Plasminogen activators u-Plasminogen activator (3.4.21.73), i.e. urokinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)

Abstract

EL INVENTO, SE REFIERE A UN PLASMIDO, QUE TIENEN UNA SECUENCIA DNA, SE UTILIZA PARA EXPRESAR UNA PROTEINA DE ETAPA PERIA DE RSCU-PA (FRACCIONES DE PROTEINAS NO GLICOSILADAS DE PROOROQUINADA DE MONOCADENA) EN ENTEROBASTERIAS E COLI Y QUE PERMANECEN INALTERABLES DURANTE LA APLICACION DEL PLASMIDO; SE OBTIENE CON LAS SECUENCIAS DNA SINTETICAS DE PLASMIDOS USUALES EN EL COMERCIO, QUE PRESENTA UN OPERADOR QUE LLEVA UN PROMOTOR SINTETICO Y REGULABLE, UNA SECUENCIA SHUNE APERTURA, UN GEN ESTRUCTURAL SINTETICO Y UN TRANSFORMADOR DE GEN ESTRUCTURAL QUE SE MUEVE HACIA ABAJO Y SE CARACTERIZA PORQUE LA PROTEINA DE ETAPA PERTENECE AL RSCU-PA Y EXPRESADA CON RELACION ELEVADA, SE UTILIZA PARA EL PLIEGUE POR RETROCESO DE RSCU-PA Y UTILIZARLA TERAPEUTICAMENTE COMO ACTIVADOR DE PLASMINOGENO Y LA PROTEINA DE ETAPA PREVIA SE DISOCIA EN FORMA CONOCIDA, PARA LA OBTENCION DE PROTEINA OROQUINADA NO GLICOSADA Y DE DOBLE CADENA (RFCU-PA) DE FORMA CONOCIDA.
ES90112400T 1989-07-19 1990-06-29 Plasmidos, su procedimiento de preparacion y su uso en la obtencion de un activador de plasminogeno. Expired - Lifetime ES2083399T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE3923866 1989-07-19

Publications (1)

Publication Number Publication Date
ES2083399T3 true ES2083399T3 (es) 1996-04-16

Family

ID=6385369

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90112400T Expired - Lifetime ES2083399T3 (es) 1989-07-19 1990-06-29 Plasmidos, su procedimiento de preparacion y su uso en la obtencion de un activador de plasminogeno.

Country Status (30)

Country Link
US (1) US5637503A (es)
EP (1) EP0408945B1 (es)
JP (1) JP3236284B2 (es)
KR (1) KR0167761B1 (es)
AT (1) ATE131534T1 (es)
AU (1) AU631662B2 (es)
CA (1) CA2020656C (es)
DD (1) DD298426A5 (es)
DE (2) DE4020438A1 (es)
DK (1) DK0408945T3 (es)
ES (1) ES2083399T3 (es)
FI (1) FI102386B1 (es)
GR (1) GR3019294T3 (es)
HK (1) HK1005193A1 (es)
HR (1) HRP920596B1 (es)
HU (1) HU214243B (es)
IE (1) IE901849A1 (es)
IL (1) IL94529A (es)
LT (1) LT3611B (es)
LV (1) LV10120B (es)
MT (1) MTP1063B (es)
NO (1) NO304952B1 (es)
NZ (1) NZ234542A (es)
PL (1) PL166199B1 (es)
PT (1) PT94776B (es)
RU (1) RU2073720C1 (es)
SI (1) SI9011347B (es)
SK (1) SK280028B6 (es)
YU (1) YU48371B (es)
ZA (1) ZA904006B (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2067226A1 (es) * 1969-11-27 1971-08-20 Cefilac
DE4101736A1 (de) * 1991-01-22 1992-07-23 Gruenenthal Gmbh Neue als plasminogenaktivatoren einsetzbare polypeptide, dafuer codierende plasmide und verfahren zu deren herstellung und deren verwendung
DE4440892A1 (de) * 1994-11-17 1996-05-23 Gruenenthal Gmbh Proteine mit fibrinolytischen und gerinnungshemmenden Eigenschaften
DE4442665A1 (de) * 1994-11-30 1996-06-05 Gruenenthal Gmbh Chimäre Proteine mit fibrinolytischen und thrombinhemmenden Eigenschaften
KR100467034B1 (ko) * 1996-12-27 2006-04-07 주식회사 엘지생활건강 압축성형타입오일케익화장료및그의제조방법
KR100372233B1 (ko) * 1997-03-12 2003-06-11 주식회사 코리아나화장품 미백 파우더, 그의 제조방법 및 그를 함유하는 메이크업 미백 화장료
KR100341638B1 (ko) * 2000-03-17 2002-06-22 유상옥,송운한 기능성 표면처리 분체의 제조 방법 및 이를 함유하는메이크업 화장료 조성물
JP4124639B2 (ja) * 2002-12-17 2008-07-23 株式会社日本触媒 大腸菌を用いたｓ−ヒドロキシニトリルリアーゼの製造方法
US7906276B2 (en) * 2004-06-30 2011-03-15 Kimberly-Clark Worldwide, Inc. Enzymatic detection techniques
JP2010525812A (ja) 2007-05-02 2010-07-29 メリアル リミテッド 発現及び安定性が改善されたｄｎａプラスミド
RU2553533C2 (ru) * 2013-10-23 2015-06-20 Общество с ограниченной ответственностью "Международный биотехнологический центр "Генериум" ("МБЦ "Генериум") Способ выделения и очистки рекомбинантной человеческой проурокиназы м5
CN104555732A (zh) * 2015-01-18 2015-04-29 河南省中原起重机械总厂 一种多功能门式起重机

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4370417A (en) * 1980-04-03 1983-01-25 Abbott Laboratories Recombinant deoxyribonucleic acid which codes for plasminogen activator
US4558010A (en) * 1980-04-03 1985-12-10 Abbott Laboratories Recombinant deoxyribonucleic acid which codes for plasminogen activator and method of making plasminogen activator protein therefrom
GR78180B (es) * 1982-04-15 1984-09-26 Genentech Inc
US4710464A (en) * 1984-09-27 1987-12-01 Eli Lilly And Company Transcription terminators
AU5390286A (en) * 1985-01-25 1986-08-13 Sagami Chemical Research Center Stabilized human prourokinase
FR2594845B1 (fr) * 1986-02-21 1989-12-01 Genetica Preparation par voie microbiologique de l'activateur tissulaire humain du plasminogene (t-pa) et conversion de l'enzyme ainsi obtenue en sa forme active
FI100106B (fi) * 1986-12-05 1997-09-30 Novartis Ag Menetelmä plasminogeenin yksisäikeisen yhdistelmäaktivaattorin valmist amiseksi
HU208712B (en) * 1987-08-10 1993-12-28 Lepetit Spa Method for preparing single or double strained glycolised human urokinase and pharmaceutical composition comprising same
EP0372005A4 (en) * 1987-10-30 1990-06-26 Oncogen EXPRESSION SYSTEMS FOR THE PREPARATION OF POLYPEPTIDES IN PROKARYOTIC CELLS.

Also Published As

Publication number Publication date
YU134790A (sh) 1992-12-21
HRP920596B1 (en) 1999-12-31
FI102386B (fi) 1998-11-30
DE4020438A1 (de) 1991-01-31
AU5883290A (en) 1991-01-24
FI903640A0 (fi) 1990-07-18
KR0167761B1 (ko) 1999-01-15
RU2073720C1 (ru) 1997-02-20
ATE131534T1 (de) 1995-12-15
JPH0365189A (ja) 1991-03-20
PL166199B1 (pl) 1995-04-28
LV10120A (lv) 1994-05-10
JP3236284B2 (ja) 2001-12-10
FI102386B1 (fi) 1998-11-30
PT94776B (pt) 1997-09-30
US5637503A (en) 1997-06-10
LTIP776A (en) 1995-01-31
DD298426A5 (de) 1992-02-20
NO304952B1 (no) 1999-03-08
HRP920596A2 (en) 1998-06-30
HU903970D0 (en) 1990-11-28
KR910003105A (ko) 1991-02-26
SI9011347B (sl) 1999-02-28
PL286087A1 (en) 1991-03-11
AU631662B2 (en) 1992-12-03
NZ234542A (en) 1991-09-25
MTP1063B (en) 1991-04-08
LV10120B (en) 1994-10-20
DK0408945T3 (da) 1996-04-09
ZA904006B (en) 1991-03-27
IL94529A0 (en) 1991-03-10
HU214243B (hu) 1998-03-02
EP0408945B1 (de) 1995-12-13
GR3019294T3 (en) 1996-06-30
LT3611B (en) 1995-12-27
SK306790A3 (en) 1999-07-12
EP0408945A1 (de) 1991-01-23
PT94776A (pt) 1991-03-20
SI9011347A (sl) 1998-04-30
SK280028B6 (sk) 1999-07-12
IE901849A1 (en) 1991-06-19
YU48371B (sh) 1998-07-10
HUT54211A (en) 1991-01-28
HK1005193A1 (en) 1998-12-24
NO902720D0 (no) 1990-06-19
IL94529A (en) 1997-11-20
DE59009961D1 (de) 1996-01-25
CA2020656A1 (en) 1991-01-20
CA2020656C (en) 2001-05-22
NO902720L (no) 1991-01-21

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