DD158917A5 - Verfahren zur herstellung einer ein amylase codierendes gen enthaltenden rekombinanten dns - Google Patents

Verfahren zur herstellung einer ein amylase codierendes gen enthaltenden rekombinanten dns Download PDF

Info

Publication number
DD158917A5
DD158917A5 DD81227656A DD22765681A DD158917A5 DD 158917 A5 DD158917 A5 DD 158917A5 DD 81227656 A DD81227656 A DD 81227656A DD 22765681 A DD22765681 A DD 22765681A DD 158917 A5 DD158917 A5 DD 158917A5
Authority
DD
German Democratic Republic
Prior art keywords
lambda
amylase
sup
phage
plasmid
Prior art date
Application number
DD81227656A
Other languages
German (de)
English (en)
Inventor
Charles A Colson
Pierre E Cornelis
Colette S Digneffe
Raoul G P Walon
Corrine Walon
Original Assignee
Cpc International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26274508&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DD158917(A5) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Cpc International Inc filed Critical Cpc International Inc
Publication of DD158917A5 publication Critical patent/DD158917A5/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2414Alpha-amylase (3.2.1.1.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2414Alpha-amylase (3.2.1.1.)
    • C12N9/2417Alpha-amylase (3.2.1.1.) from microbiological source
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2425Beta-amylase (3.2.1.2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2451Glucanases acting on alpha-1,6-glucosidic bonds
    • C12N9/2457Pullulanase (3.2.1.41)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01041Pullulanase (3.2.1.41)
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/8215Microorganisms
    • Y10S435/822Microorganisms using bacteria or actinomycetales
    • Y10S435/832Bacillus
    • Y10S435/839Bacillus subtilis
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/8215Microorganisms
    • Y10S435/822Microorganisms using bacteria or actinomycetales
    • Y10S435/848Escherichia
    • Y10S435/849Escherichia coli

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DD81227656A 1980-02-15 1981-02-16 Verfahren zur herstellung einer ein amylase codierendes gen enthaltenden rekombinanten dns DD158917A5 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB8005184 1980-02-15
GB8011842 1980-04-10

Publications (1)

Publication Number Publication Date
DD158917A5 true DD158917A5 (de) 1983-02-09

Family

ID=26274508

Family Applications (3)

Application Number Title Priority Date Filing Date
DD81227656A DD158917A5 (de) 1980-02-15 1981-02-16 Verfahren zur herstellung einer ein amylase codierendes gen enthaltenden rekombinanten dns
DD81245456A DD207221A5 (de) 1980-02-15 1981-02-16 Verfahren zur herstellung von amylase mit hilfe genetisch erzeugter mikroorganismen, die rekombinante dns enthalten
DD81245452A DD207553A5 (de) 1980-02-15 1981-02-16 Verfahren zur enzymatischen hydrolyse von staerke unter verwendung von amylase

Family Applications After (2)

Application Number Title Priority Date Filing Date
DD81245456A DD207221A5 (de) 1980-02-15 1981-02-16 Verfahren zur herstellung von amylase mit hilfe genetisch erzeugter mikroorganismen, die rekombinante dns enthalten
DD81245452A DD207553A5 (de) 1980-02-15 1981-02-16 Verfahren zur enzymatischen hydrolyse von staerke unter verwendung von amylase

Country Status (16)

Country Link
US (1) US4469791A (show.php)
EP (1) EP0034470B1 (show.php)
CA (1) CA1183090A (show.php)
DD (3) DD158917A5 (show.php)
DE (1) DE3177286T2 (show.php)
DK (1) DK162776B (show.php)
ES (1) ES8202362A1 (show.php)
FI (1) FI70424C (show.php)
GB (1) GB2069503B (show.php)
GR (1) GR73678B (show.php)
HU (1) HU195533B (show.php)
IE (1) IE50919B1 (show.php)
IL (1) IL61982A (show.php)
KE (1) KE3310A (show.php)
RU (1) RU1838410C (show.php)
YU (2) YU43634B (show.php)

Families Citing this family (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU545912B2 (en) * 1980-03-10 1985-08-08 Cetus Corporation Cloned heterologous jive products in bacillies
US4874702A (en) * 1980-09-08 1989-10-17 Biogen, Inc. Vectors and methods for making such vectors and for expressive cloned genes
FI64813C (fi) * 1980-12-31 1984-01-10 Ilkka Antero Palva Foerfarande foer producering av ett utvalt aeggviteaemne och vid foerfarandet anvaenda rekombinantplasmidvektorer
IE52434B1 (en) * 1981-01-15 1987-10-28 Cpc International Inc A process for cloning the gene coding for a thermostable alpha-amylase into escherichia coli and bacillus subtilis
US4493893A (en) * 1981-01-15 1985-01-15 Cpc International Inc. Process for cloning the gene coding for a thermostable alpha-amylase into Escherichia coli and Bacillus subtilis
US5525484A (en) * 1981-01-16 1996-06-11 Genome Therapeutics Corp. Recombinant DNA means and method for producing rennin, prorenin and pre-prorennin
EP0076037B1 (en) * 1981-09-02 1988-12-07 Biogen, Inc. Amplified expression of dna sequences
US5254463A (en) * 1981-09-18 1993-10-19 Genentech, Inc. Method for expression of bovine growth hormone
US4508826A (en) * 1982-04-15 1985-04-02 Merck & Co., Inc. Bacteriophage DNA cloning vector TG1 and microorganisms containing TG1
US4460689A (en) * 1982-04-15 1984-07-17 Merck & Co., Inc. DNA Cloning vector TG1, derivatives, and processes of making
FR2533583A1 (fr) * 1982-09-24 1984-03-30 Centre Nat Rech Scient Nouvel adn recombinant utile dans la preparation d'a-amylase
FR2537602B2 (fr) * 1982-09-24 1986-10-24 Centre Nat Rech Scient Nouvel adn recombinant perfectionne utile dans la preparation d'a-amylase par bacillus subtilis
EP0108301B2 (en) * 1982-11-01 1993-09-01 SOLVAY ENZYMES, INC. (a Delaware corporation) Method of heterologous cloning of gene in bacillus microorganism
NO840200L (no) * 1983-01-28 1984-07-30 Cefus Corp Glukoamylase cdna.
DK135983D0 (da) * 1983-03-25 1983-03-25 Novo Industri As Maltogen amylaseenzymprodukt og fremgangsmade til dets fremstilling og anvendelse
JPS59196092A (ja) * 1983-04-25 1984-11-07 Sanraku Inc 組換えプラスミドによる耐熱性α−アミラ−ゼの新規製造法
ES8608579A1 (es) * 1983-07-06 1986-06-16 Gist Brocades Nv Procedimiento para introducir dna en un anfitrion de cepa de microorganismo unicelular
US5624829A (en) * 1984-07-03 1997-04-29 Gist-Brocades, B.V. Transformed industrial bacillus strains and methods for making and using them
US4578352A (en) * 1983-07-13 1986-03-25 Cpc International Inc. Novel thermostable, aciduric alpha-amylase and method for its production
FR2556008B1 (fr) * 1983-12-06 1987-06-26 Centre Nat Rech Scient Vecteurs plasmidiques de clonage et d'expression d'une proteine dans un micro-organisme, comportant au moins le promoteur d'expression de la b-glucosidase dans les levures; micro-organismes les contenant; procede de fermentation et enzymes obtenues
GB8333797D0 (en) * 1983-12-19 1984-01-25 Searle & Co Starch utilization
PH23920A (en) * 1983-12-20 1990-01-23 Cetus Corp Glucoamylase cdna
GB8414272D0 (en) * 1984-06-05 1984-07-11 Cpc International Inc Enzymatic hydrolysis
GB8414271D0 (en) * 1984-06-05 1984-07-11 Cpc International Inc Recombinant dna
JPH062061B2 (ja) * 1984-06-14 1994-01-12 協和醗酵工業株式会社 N−アセチルノイラミン酸リア−ゼの製法
US5489529A (en) * 1984-07-19 1996-02-06 De Boer; Herman A. DNA for expression of bovine growth hormone
JPH0630586B2 (ja) * 1984-12-15 1994-04-27 サントリー株式会社 グルコアミラ−ゼ遺伝子
JPS61162183A (ja) * 1985-01-07 1986-07-22 Agency Of Ind Science & Technol プルラナ−ゼ様酵素の製造法
AU5668486A (en) * 1985-03-29 1986-10-23 Biotechnica International Inc. Secretion vector
DE3688920T4 (de) * 1985-07-03 1995-08-31 Genencor Int Hybride Polypeptide und Verfahren zu deren Herstellung.
JPH0616711B2 (ja) * 1985-09-27 1994-03-09 メルシャン株式会社 高温で自律増殖するプラスミド
SE8505027D0 (sv) * 1985-10-24 1985-10-24 Kabigen Ab A lytic virulent phage, a hostcell containing same and a process for protein production
US5017477A (en) * 1985-10-25 1991-05-21 Biotechnica International, Inc. Enhancing DNA sequencies derived from the sacQ gene
FR2598431B1 (fr) * 1986-05-09 1990-02-09 Transgene Sa Souches de saccharomyces produisant de l'alpha-amylase
US4962026A (en) * 1986-09-15 1990-10-09 Enzyme Bio-Systems, Ltd. Process for the production of panosyl derivatives
HUT50877A (en) * 1987-02-27 1990-03-28 Gist Brocades Nv Process for producing stable gene amplification in chromosomal dna of procaryote microorganisms
JPH01218589A (ja) * 1988-02-26 1989-08-31 Juzo Udaka 耐熱性β−アミラーゼ遺伝子
US5171673A (en) * 1988-07-18 1992-12-15 Biotechnica International, Inc. Expression of heterologous dna using the bacillus coagulans amylase gene
US5209938A (en) * 1989-09-13 1993-05-11 Cpc International Inc. Method for retarding staling of baked goods
US5583039A (en) * 1990-06-07 1996-12-10 Sunkyong Industries Ltd. Escherichia coli containing a gene encoding a maltogenic amylase BLMA of Bacillus licheniformis and gene product produced by same
CA2088592A1 (en) * 1990-08-01 1992-02-02 Garabed Antranikian Thermostable pullulanases
DE4029844A1 (de) * 1990-09-20 1992-03-26 Boehringer Mannheim Gmbh Carbamoylsarcosinhydrolase, kloniert
DK47291D0 (da) * 1991-03-15 1991-03-15 Novo Nordisk As Pullulanase
DE69433032T2 (de) * 1993-05-19 2004-06-03 Kao Corp. VERFLÜSSIGENDE -[alpha]-AMYLASE, VERFAHREN ZU IHRER HERSTELLUNG, UND SIE ENTHALTENDE WASCHMITTELZUSAMMENSETZUNG
DK0722501T3 (da) * 1993-10-05 2000-08-21 Miller Brewing Klonet pullulanase
US6300115B1 (en) 1998-05-18 2001-10-09 Enzyme Bio-Systems Ltd. Pullulanase expression constructs containing α-amylase promoter and leader sequences
US7220542B2 (en) * 2000-07-17 2007-05-22 Van Den Brink Johannes Maarten Expression cloning in filamentous fungi
CN115029336B (zh) * 2022-01-10 2025-05-16 昆明理工大学 一种具有洗涤剂抗性的普鲁兰酶PulY103B及其应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1521032A (en) * 1974-08-08 1978-08-09 Ici Ltd Biological treatment
US4237224A (en) * 1974-11-04 1980-12-02 Board Of Trustees Of The Leland Stanford Jr. University Process for producing biologically functional molecular chimeras
JPS5811998B2 (ja) * 1975-12-19 1983-03-05 マルオ ブンジ シンキナアミラ−ゼノセイゾウホウホウ
NZ187300A (en) * 1977-05-27 1982-08-17 Univ California Dna transfer vector and micro-organism modified to contain a nucleotide sequence equivalent to the gene of a higher organism
IL58308A (en) * 1978-10-23 1983-10-31 Upjohn Co Process for preparing glucose isomerase using a recombinant plasmid

Also Published As

Publication number Publication date
GB2069503A (en) 1981-08-26
DK62881A (da) 1981-08-16
ES499391A0 (es) 1982-01-01
IE50919B1 (en) 1986-08-20
DE3177286D1 (de) 1992-09-17
ES8202362A1 (es) 1982-01-01
GB2069503B (en) 1983-04-20
EP0034470B1 (en) 1992-08-12
IE810297L (en) 1981-08-15
DK162776B (da) 1991-12-09
EP0034470A3 (en) 1982-02-10
DE3177286T2 (de) 1992-12-10
YU86783A (en) 1985-10-31
FI70424C (fi) 1986-09-19
YU43634B (en) 1989-10-31
GR73678B (show.php) 1984-03-30
US4469791A (en) 1984-09-04
KE3310A (en) 1983-08-26
EP0034470A2 (en) 1981-08-26
DD207553A5 (de) 1984-03-07
FI810425L (fi) 1981-08-16
YU37781A (en) 1985-03-20
IL61982A (en) 1984-01-31
DD207221A5 (de) 1984-02-22
RU1838410C (ru) 1993-08-30
IL61982A0 (en) 1981-02-27
YU44739B (en) 1991-02-28
CA1183090A (en) 1985-02-26
HU195533B (en) 1988-05-30
FI70424B (fi) 1986-03-27

Similar Documents

Publication Publication Date Title
DD158917A5 (de) Verfahren zur herstellung einer ein amylase codierendes gen enthaltenden rekombinanten dns
DE69635419T2 (de) Alkalophile und thermophile mikroorganismen und damit erhaltene enzyme
DE3883041T2 (de) Stabile Genamplifizierung in prokaryotischer chromosonaler DNA.
DE10131441A1 (de) Eine neue Gruppe von alpha-Amylasen sowie ein Verfahren zur Identifizierung und Gewinnung neuer alpha-Amylasen
EP0459385B1 (de) Maltopentaose produzierende Amylasen
DE69434807T2 (de) Methode und system zur erhöhten produktion von kommerziell wichtigen exoproteinen in gram-positiven bakterien
DE69028769T2 (de) Thermostabile (1,3-1,4)-beta-glucanase
EP1282716A1 (de) Verfahren zur herstellung rekombinanter proteine durch gram-negative bakterien
DE60036647T2 (de) Klonierung und expression einer extrazellulären säurebeständigen lipase aus yarrowia lipolytica
EP2809816A1 (de) Expressionsverfahren
DD208988A5 (de) Verfahren zum aufbau eines chimaeren plasmids mit einer genkodierung fuer eine thermostabile alpha-amylase
DE69012134T2 (de) Fuer lipase kodierende dns und lipasemodulator.
DE69111456T2 (de) Eine methode für die schnelle selektion von effizienten sekretionsvektoren.
DE3423899C2 (show.php)
EP0751218B1 (de) Saccharose-Stoffwechsel-Mutanten
DE69105188T2 (de) Asporogener Stamm von Bacillus substilis und seine Verwendung als Wirt in der Herstellung von heterologen Produkten.
DE2717333A1 (de) Hitze- und saeurebestaendiges alpha- amylaseenzym, verfahren zu seiner herstellung und seine verwendung
DE4231764A1 (de) Verfahren zur chromosomalen Integration eines Produkt-Gens
DD226012A1 (de) Verfahren zur herstellung von bacillus-beta-1,3-1,4-glucanase
DE4425058C2 (de) Verfahren zur Herstellung einer thermostabilen Glukoamylase
DE3687872T2 (de) Plasmide und transformanten.
DE3486163T2 (de) Plasmide, Verfahren zu ihrer Herstellung, sie enthaltende Mikroorganismen und Methoden zur extrazellulären Herstellung von Xylanase durch Züchten dieser Mikroorganismen.
DE4136389A1 (de) Verfahren zur herstellung von glutarylacylase in grossen mengen
DE69429479T2 (de) Dna fragment welches ein gen für eine alkaline pullulanase beinhaltet
DE102017215015A1 (de) Verfahren zur verbesserten Expression von Enzymen