CZ308081B6 - Rekombinantní glykoproteiny a způsob jejich výroby - Google Patents

Rekombinantní glykoproteiny a způsob jejich výroby Download PDF

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CZ308081B6
CZ308081B6 CZ2008-404A CZ2008404A CZ308081B6 CZ 308081 B6 CZ308081 B6 CZ 308081B6 CZ 2008404 A CZ2008404 A CZ 2008404A CZ 308081 B6 CZ308081 B6 CZ 308081B6
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fucosyltransferase
glcnac
sequence
activity
plant cells
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Friedrich Altmann
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Friedrich Altmann
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Abstract

Předkládaný vynález se týká rekombinantních glykoproteinů, které byly připraveny v rostlinách nebo buňkách rostlin, ve kterých je utlumena nebo zcela potlačena endogenní produkce GlcNAc-αl,3-fukosyltransferázy a jejichž peptidová sekvence má méně než 50 % αl,3-vázaných fukózových zbytků ve srovnání se zbytky přítomnými v glykoproteinech exprimovaných v rostlinných systémech s neredukovanou fukosyltransferázovou aktivitou, přičemž rekombinantní glykoproteiny mají neredukovaný GlcNAc-konec molekuly a způsob jejich výroby.

Claims (6)

1. Způsob produkce rekombinantních glykoproteinů vyznačující se tím, že zahrnuje produkci rekombinantního glykoproteinů v rostlinách nebo rostlinných buňkách, ve kterých byla produkce GlcNAc-al,3-fukosyltransferázy potlačena nebo zcela vyloučena a pro které platí, že jejich endogenní aktivita GlcNAc-al,3-fukosyltransferázy je menší než 50 % aktivity GlcNAc-al,3-fukosyltransferázy přítomné v přírodních rostlinách nebo rostlinných buňkách, přičemž jejich GlcNAc-al,3-fukosyltransferáza je kódována molekulou DNA zahrnující sekvenci podle sekvence ID č. 1 s otevřeným čtecím rámcem od páru bází 211 do páru bází 1740 nebo sekvenci, která je alespoň z 50 % homologní s výše uvedenou sekvencí nebo která hybridizuje za stringentních podmínek s výše uvedenou sekvencí nebo která zahrnuje sekvenci, která degeneruje s výše uvedenou DNA sekvencí kvůli genetickému kódu.
2. Způsob podle nároku 1, vyznačující se tím, že rekombinantní glykoproteiny jsou lidské proteiny, zejména proteiny pro medicinální použití.
3. Způsob podle nároku 1 nebo 2, vyznačující se tím, že endogenní aktivita GlcNAc-al,3- fukosyltransferázy je menší než 20 % aktivity GlcNAc-al,3-fukosyltransferázy přítomné v přírodních rostlinách nebo rostlinných buňkách.
4. Způsob podle kteréhokoli z nároků 1 až 3, vyznačující se tím, že endogenní aktivita GlcNAc-al,3fukosyltransferázy je 0 % aktivity GlcNAc-al,3-fukosyltransferázy přítomné v přírodních rostlinách nebo rostlinných buňkách.
5. Způsob podle kteréhokoli z nároků 1 až 4, vyznačující se tím, že snížení aktivity GlcNAc-al,3fukosyltransferázy je díky antisense inhibici, přičemž je použit polynukleotid, který je alespoň částečně komplementární k sekvenci molekuly DNA.
6. Způsob podle kteréhokoli z nároků 1 až 4, vyznačující se tím, že snížení aktivity GlcNAc-al,3fukosyltransferázy je díky knock-out mutaci sekvence molekuly DNA.
CZ2008-404A 1999-02-18 2000-02-17 Rekombinantní glykoproteiny a způsob jejich výroby CZ308081B6 (cs)

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AT0027099A AT408446B (de) 1999-02-18 1999-02-18 Fucosyltransferase-gen

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CZ2008-404A CZ308081B6 (cs) 1999-02-18 2000-02-17 Rekombinantní glykoproteiny a způsob jejich výroby
CZ20012943A CZ299622B6 (cs) 1999-02-18 2000-02-17 Molekula DNA kódující fukosyltransferázu

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EP (3) EP1642979A1 (cs)
JP (2) JP5514386B2 (cs)
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Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU771908C (en) 1998-12-09 2005-03-10 Phyton Holdings, Llc A method for manufacturing glycoproteins having human-type glycosylation
AT408446B (de) * 1999-02-18 2001-11-26 Altmann Friedrich Dr Fucosyltransferase-gen
ES2571230T3 (es) 1999-04-09 2016-05-24 Kyowa Hakko Kirin Co Ltd Procedimiento para controlar la actividad de una molécula inmunofuncional
IL149271A0 (en) 1999-10-26 2002-11-10 Plant Res Int Bv Mammalian-type glycosylation in plants
US6946292B2 (en) 2000-10-06 2005-09-20 Kyowa Hakko Kogyo Co., Ltd. Cells producing antibody compositions with increased antibody dependent cytotoxic activity
ZA200306406B (en) 2001-01-19 2004-09-06 Dow Chemical Co Method for secretory production of glycoprotein having human-type sugar chain using plant cell.
NZ534881A (en) 2002-03-19 2006-09-29 Plant Res Internat B Mammalian GnTIII expression in plants
KR101196023B1 (ko) 2002-03-19 2012-10-30 스티칭 디엔스트 랜드보위쿤디그 온데조에크 식물에서 글리칸 프로세싱의 최적화
AU2003236020B2 (en) * 2002-04-09 2009-03-19 Kyowa Hakko Kirin Co., Ltd. Cell with depression or deletion of the activity of protein participating in GDP-fucose transport
CL2003002461A1 (es) 2002-11-27 2005-01-07 Dow Chemical Company Agroscien Inmunoglobulina que comprende al menos un glicano afucosilado, composicion que la contiene, secuencia nucleotidica y vector que la comprende, procedimiento para producir dicha inmunoglobulina en plantas.
EP1510584B1 (en) 2003-08-11 2009-03-18 Greenovation Biotech GmbH Expression promoting sequences from mosses and their uses
FI20055398A0 (fi) 2005-07-08 2005-07-08 Suomen Punainen Risti Veripalv Menetelmä solupopulaatioiden evaluoimiseksi
AU2007205935B2 (en) 2006-01-17 2013-07-11 Synthon Biopharmaceuticals B.V. Compositions and methods for humanization and optimization of N-glycans in plants
US20090060921A1 (en) * 2006-01-17 2009-03-05 Biolex Therapeutics, Inc. Glycan-optimized anti-cd20 antibodies
CN102094020B (zh) * 2007-01-15 2012-11-28 燕秋 用于抑制LeY糖抗原合成的岩藻糖基转移酶I的RNA干涉序列及重组干涉质粒
CN102094019B (zh) * 2007-01-15 2012-11-28 燕秋 用于抑制LeY糖抗原合成的岩藻糖基转移酶Ⅳ的RNA干涉序列及重组干涉质粒
AU2008237632B2 (en) 2007-04-17 2014-01-16 Stichting Dienst Landbouwkundig Onderzoek Mammalian-type glycosylation in plants by expression of non-mammalian glycosyltransferases
JP5766947B2 (ja) * 2007-09-07 2015-08-19 チルドレンズ ホスピタル メディカル センター 臨床サンプルにおける分泌性のルイス抗原およびシアル化抗原のレベルの疾患リスクの予測指標としての使用
US20100242128A1 (en) * 2007-10-31 2010-09-23 Bayer BioSceince NV Method to produce modified plants with altered n-glycosylation pattern
EP2166085A1 (en) 2008-07-16 2010-03-24 Suomen Punainen Risti Veripalvelu Divalent modified cells
ES2663627T3 (es) 2010-10-11 2018-04-16 Jennewein Biotechnologie Gmbh Fucosiltransferasas novedosas y sus aplicaciones
KR101293658B1 (ko) * 2010-12-30 2013-08-07 대한민국 알파-1,3-푸코실트랜스퍼라아제 및/또는 베타-1,2-자일로실트렌스퍼라아제 유전자를 포함하는 벼 형질전환용 벡터 및 형질전환된 벼
ES2439507T3 (es) * 2011-01-20 2014-01-23 Jennewein Biotechnologie Gmbh Fucosiltransferasas novedosas y sus aplicaciones
EP2789686A1 (en) 2013-04-11 2014-10-15 Greenovation Biotech GmbH Expression of phosphorylated glycoproteins in plants
EP3050973A1 (en) * 2015-01-30 2016-08-03 Jennewein Biotechnologie GmbH Fermentation process for producing monosaccharides in free form from nucleotide-activated sugars
CN110317799A (zh) * 2019-07-18 2019-10-11 江南大学 一种人源岩藻糖转移酶8的原核表达方法及其产品
EP3871687A1 (en) 2020-02-27 2021-09-01 eleva GmbH Enzyme replacement therapy for treating pompe disease

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997009421A1 (en) * 1995-09-08 1997-03-13 The Regents Of The University Of Michigan Chimeric fucosyltransferase polypeptides and nucleic acid sequences encoding them

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5272066A (en) 1986-03-07 1993-12-21 Massachusetts Institute Of Technology Synthetic method for enhancing glycoprotein stability
JP3756946B2 (ja) 1993-03-29 2006-03-22 協和醗酵工業株式会社 α１，３−フコシルトランスフェラーゼ
US6509149B2 (en) * 1995-06-06 2003-01-21 Hybridon, Inc. HPV-specific oligonucleotides
AT408446B (de) * 1999-02-18 2001-11-26 Altmann Friedrich Dr Fucosyltransferase-gen

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997009421A1 (en) * 1995-09-08 1997-03-13 The Regents Of The University Of Michigan Chimeric fucosyltransferase polypeptides and nucleic acid sequences encoding them

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
GRABENHORST E. et al.: „Genetic engineering of recombinant glycoproteins and the glycosylation pathway in mammalian host cells", Glycoconjugate Journal, vol. 16, no. 2, únor 1999, str. 81 - 97, ISSN 0282-0080 *
LEROUGE P. et al.: „N-Glycoprotein biosynthesis in plants: recent developments and future trends", Plant Molecular Biology, vol. 38, 1998, str. 31 - 48, ISSN 0167-4412 *
SCHAEWEN VON A. et al.: „Isolation of a mutant Arabidopsis plant that lacks N-acetyl-glucosaminyl transferase I and is unable to synthesize Golgi-modified complex N-linked glycans", Plant Physiology, vol. 102, 1993, str. 1109 - 1118, ISSN 0032-0889 *
WAGNER R. et al.: „N-Acetyl-β-glucosaminidase accounts for differences in glycosylation of influenza virus hemagglutinin expressed in insect cells from baculovirus vector ", Journal of Virology, vol. 70, no. 6, 1996, str. 4103 - 4109, ISSN 0022-538X *

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US8895806B2 (en) 2014-11-25
EP2062977A1 (de) 2009-05-27
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CZ299622B6 (cs) 2008-09-24
IL144777A (en) 2007-12-03
NO20013993D0 (no) 2001-08-16
EP2062977B1 (de) 2014-01-08
PL363438A1 (en) 2004-11-15
HRP20010681A2 (en) 2003-08-31
WO2000049153A1 (de) 2000-08-24
AU2646000A (en) 2000-09-04
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AU771995B2 (en) 2004-04-08
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