DE11151437T1 - Genetisch aufbereitetes Gellan - Google Patents

Genetisch aufbereitetes Gellan Download PDF

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Publication number
DE11151437T1
DE11151437T1 DE11151437T DE11151437T DE11151437T1 DE 11151437 T1 DE11151437 T1 DE 11151437T1 DE 11151437 T DE11151437 T DE 11151437T DE 11151437 T DE11151437 T DE 11151437T DE 11151437 T1 DE11151437 T1 DE 11151437T1
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DE
Germany
Prior art keywords
isolated
genomic dna
purified polynucleotide
sphingomonas elodea
arylsulfatase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
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DE11151437T
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English (en)
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DE11151437T8 (de
Inventor
Joseph M. Cleary
J. COLEMAN Russell
Nancy E. Harding
Yamini Patel
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CP Kelco US Inc
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CP Kelco US Inc
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Publication of DE11151437T1 publication Critical patent/DE11151437T1/de
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Publication of DE11151437T8 publication Critical patent/DE11151437T8/de
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
    • A23C9/137—Thickening substances
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
    • A23C9/154—Milk preparations; Milk powder or milk powder preparations containing additives containing thickening substances, eggs or cereal preparations; Milk gels
    • A23C9/1544—Non-acidified gels, e.g. custards, creams, desserts, puddings, shakes or foams, containing eggs or thickening or gelling agents other than sugar; Milk products containing natural or microbial polysaccharides, e.g. cellulose or cellulose derivatives; Milk products containing nutrient fibres
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/20—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
    • A23L29/269—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of microbial origin, e.g. xanthan or dextran
    • A23L29/272—Gellan
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L5/00—Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01031—Beta-glucuronidase (3.2.1.31)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Food Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nutrition Science (AREA)
  • Materials Engineering (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Jellies, Jams, And Syrups (AREA)
  • Medicinal Preparation (AREA)
  • Tea And Coffee (AREA)

Abstract

Eine Zusammensetzung, die Gellangummi beinhaltet, im Wesentlichen frei von Arylsulfataseprotein.

Claims (14)

  1. Eine Zusammensetzung, die Gellangummi beinhaltet, im Wesentlichen frei von Arylsulfataseprotein.
  2. Eine Zusammensetzung, die Gellangummi beinhaltet, im Wesentlichen frei von β-Glucoronidaseprotein.
  3. Eine Zusammensetzung, die Gellangummi beinhaltet, im Wesentlichen frei von Arylsulfatase- und β-Glucoronidaseproteinen.
  4. Zusammensetzung gemäß einem der Ansprüche 1 bis 3, die eine Kulturbrühe von Sphingomonas elodea ist.
  5. Zusammensetzung gemäß einem der Ansprüche 1 bis 3, die eine gefällte Kulturbrühe von Sphingomonas elodea ist.
  6. Zusammensetzung gemäß einem der Ansprüche 1 bis 3, die ein sterilisiertes Milchprodukt ist.
  7. Ein isoliertes und gereinigtes Polynukleotid, das eine Arylsulfatase von Sphingomonas elodea mit einer Aminosäuresequenz gemäß SEQ ID NO: 2 codiert.
  8. Isoliertes und gereinigtes Polynukleotid gemäß Anspruch 7, das eine Nukleotidsequenz gemäß den Nukleotiden 521 bis 2176 von SEQ ID NO: 1 beinhaltet.
  9. Ein isoliertes und gereinigtes Polynukleotid, das genomische DNA von Sphingomonas elodea mit einer Deletion der gesamten oder eines Teils von dessen Arylsulfatase codierender Sequenz beinhaltet.
  10. Isoliertes und gereinigtes Polynukleotid gemäß Anspruch 9, wobei die genomische DNA die gesamte oder einen Teil der genomischen DNA von Sphingomonas elodea, die Alkylsulfatase codiert, beinhaltet.
  11. Ein isoliertes und gereinigtes Polynukleotid, das genomische DNA von Sphingomonas elodea mit einer Deletion der gesamten oder eines Teils von dessen β-Glucoronidase codierender Sequenz beinhaltet.
  12. Isoliertes und gereinigtes Polynukleotid gemäß Anspruch 11, wobei die Deletion die von Nukleotiden 1588 bis 3447, SEQ ID NO: 4 ist.
  13. Isoliertes und gereinigtes Polynukleotid gemäß Anspruch 11, wobei die genomische DNA die gesamte oder einen Teil der genomischen DNA von Sphingomonas elodea, die aufwärts von β-Glucoronidase konserviertes hypothetisches Protein codiert, beinhaltet.
  14. Isoliertes und gereinigtes Polynukleotid gemäß Anspruch 11, wobei die genomische DNA die gesamte oder einen Teil der genomischen DNA von Sphingomonas elodea, die Malatdehydrogenase codiert, beinhaltet.
DE11151437T 2004-06-21 2005-06-20 Genetisch aufbereitetes Gelan Active DE11151437T8 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US58073004P 2004-06-21 2004-06-21
US580730P 2004-06-21
EP11151437.8A EP2325324B1 (de) 2004-06-21 2005-06-20 Genetisch aufbereitetes Gelan

Publications (2)

Publication Number Publication Date
DE11151437T1 true DE11151437T1 (de) 2012-09-06
DE11151437T8 DE11151437T8 (de) 2013-04-25

Family

ID=35785733

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DE11151437T Active DE11151437T8 (de) 2004-06-21 2005-06-20 Genetisch aufbereitetes Gelan
DE11151426T Active DE11151426T8 (de) 2004-06-21 2005-06-20 Genetisch aufbereitetes Gelan

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DE11151426T Active DE11151426T8 (de) 2004-06-21 2005-06-20 Genetisch aufbereitetes Gelan

Country Status (17)

Country Link
US (1) US7361754B2 (de)
EP (4) EP2325323B1 (de)
JP (1) JP2008503237A (de)
KR (2) KR101296940B1 (de)
CN (2) CN101124329A (de)
AR (1) AR049360A1 (de)
AU (2) AU2005265200B2 (de)
BR (1) BRPI0512385A8 (de)
CA (1) CA2571518C (de)
DE (2) DE11151437T8 (de)
DK (3) DK1766601T3 (de)
ES (2) ES2365798T1 (de)
MX (1) MXPA06014955A (de)
NZ (1) NZ586761A (de)
PE (1) PE20060101A1 (de)
TW (3) TWI384950B (de)
WO (1) WO2006009938A2 (de)

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Publication number Priority date Publication date Assignee Title
NZ568554A (en) 2005-11-28 2011-12-22 Dsm Ip Assets Bv Lactase enzyme preparations yielding a clean taste
CN1932026B (zh) * 2006-09-12 2011-10-26 山东大学 一种结冷胶发酵液的脱蛋白方法
EP2510015B2 (de) * 2009-12-10 2022-10-26 DuPont Nutrition Biosciences ApS Inaktivierung von nachgeschmackinduzierenden enzymen
WO2018193146A1 (es) 2017-04-20 2018-10-25 Rodriquez Garcia Amadeo Método y proyectil para combatir nidos de insectos dañinos
JP7316758B2 (ja) * 2018-03-02 2023-07-28 合同酒精株式会社 オフフレーバーのリスクを低減できるラクターゼ調製物
CN111057711B (zh) * 2019-12-25 2022-01-18 廊坊梅花生物技术开发有限公司 鞘氨醇单胞菌工程菌及其构建方法和应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002517568A (ja) * 1998-06-10 2002-06-18 モンサント・ピーエルシー 改質ゲランガム組成物、その調製工程およびその用途
JP3379923B2 (ja) * 1999-10-01 2003-02-24 新田ゼラチン株式会社 発酵乳食品の製造方法
JP3483820B2 (ja) * 1999-12-24 2004-01-06 三栄源エフ・エフ・アイ株式会社 カルシウム強化食品
ATE394490T1 (de) 2000-03-02 2008-05-15 Cp Kelco Us Inc Mutante bakteriestämme vom genus sphingonomas die defizient in der herstellung von polyhydroxybutyrate sind, und verfahren zur klärung von sphinganen
US6663911B2 (en) * 2001-02-01 2003-12-16 C. P. Kelco U.S., Inc. Methods of making sterilized milk compositions comprising native gellan gum
JP2002238520A (ja) * 2001-02-20 2002-08-27 Toyo Seikan Kaisha Ltd 浮遊固形物含有密封容器入り飲料およびその製造方法
US7314974B2 (en) * 2002-02-21 2008-01-01 Monsanto Technology, Llc Expression of microbial proteins in plants for production of plants with improved properties

Also Published As

Publication number Publication date
AU2005265200A1 (en) 2006-01-26
AU2011200079A1 (en) 2011-01-27
TWI336244B (en) 2011-01-21
CA2571518A1 (en) 2006-01-26
US7361754B2 (en) 2008-04-22
EP1766601A4 (de) 2008-10-01
DK2325323T3 (da) 2014-02-17
EP1766601B1 (de) 2013-10-02
DK2325324T3 (da) 2015-01-12
DE11151426T1 (de) 2012-09-06
ES2364267T1 (es) 2011-08-30
AU2005265200B2 (en) 2011-06-23
AU2011200079B2 (en) 2012-02-09
JP2008503237A (ja) 2008-02-07
TWI466636B (zh) 2015-01-01
CN101921780B (zh) 2014-06-18
EP1766601B8 (de) 2013-11-06
KR101296940B1 (ko) 2013-08-14
ES2365798T1 (es) 2011-10-11
AR049360A1 (es) 2006-07-19
DE11151437T8 (de) 2013-04-25
CA2571518C (en) 2018-01-02
TWI384950B (zh) 2013-02-11
KR20070085201A (ko) 2007-08-27
TW201043142A (en) 2010-12-16
EP2325324B1 (de) 2014-10-22
EP1766601A2 (de) 2007-03-28
WO2006009938A2 (en) 2006-01-26
EP2682471B1 (de) 2017-04-19
CN101921780A (zh) 2010-12-22
MXPA06014955A (es) 2007-03-26
EP2325324A1 (de) 2011-05-25
WO2006009938A3 (en) 2007-07-19
DE11151426T8 (de) 2013-04-25
US20060003051A1 (en) 2006-01-05
TW200612833A (en) 2006-05-01
NZ586761A (en) 2011-10-28
EP2682471A1 (de) 2014-01-08
EP2325323A1 (de) 2011-05-25
BRPI0512385A8 (pt) 2017-10-03
CN101124329A (zh) 2008-02-13
TW201106867A (en) 2011-03-01
PE20060101A1 (es) 2006-02-27
BRPI0512385A (pt) 2008-03-11
EP2325323B1 (de) 2013-12-18
DK1766601T3 (da) 2014-01-13

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