CY1123197T1 - Μεθοδοι παρασκευης πολυνουκλεοτιδιου με χρηση χημικων συνθεσεων πολυσθενους αλατος κατιοντων - Google Patents

Μεθοδοι παρασκευης πολυνουκλεοτιδιου με χρηση χημικων συνθεσεων πολυσθενους αλατος κατιοντων

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Publication number
CY1123197T1
CY1123197T1 CY20201100632T CY201100632T CY1123197T1 CY 1123197 T1 CY1123197 T1 CY 1123197T1 CY 20201100632 T CY20201100632 T CY 20201100632T CY 201100632 T CY201100632 T CY 201100632T CY 1123197 T1 CY1123197 T1 CY 1123197T1
Authority
CY
Cyprus
Prior art keywords
polynucleotide
salt
chemical composition
chemical compositions
polystene
Prior art date
Application number
CY20201100632T
Other languages
Greek (el)
English (en)
Inventor
Premchandran H. Ramiya
Original Assignee
Geron Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Geron Corporation filed Critical Geron Corporation
Publication of CY1123197T1 publication Critical patent/CY1123197T1/el

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    • CCHEMISTRY; METALLURGY
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    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • CCHEMISTRY; METALLURGY
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
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    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/11Antisense
    • C12N2310/113Antisense targeting other non-coding nucleic acids, e.g. antagomirs
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/30Chemical structure
    • C12N2310/31Chemical structure of the backbone
    • C12N2310/314Phosphoramidates
    • C12N2310/3145Phosphoramidates with the nitrogen in 3' or 5'-position
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/30Chemical structure
    • C12N2310/35Nature of the modification
    • C12N2310/351Conjugate
    • C12N2310/3515Lipophilic moiety, e.g. cholesterol
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y207/00Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07Nucleotidyltransferases (2.7.7)
    • C12Y207/07049RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CY20201100632T 2015-04-23 2020-07-09 Μεθοδοι παρασκευης πολυνουκλεοτιδιου με χρηση χημικων συνθεσεων πολυσθενους αλατος κατιοντων CY1123197T1 (el)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562151891P 2015-04-23 2015-04-23
PCT/US2016/028657 WO2016172346A1 (en) 2015-04-23 2016-04-21 Methods of polynucleotide preparation using multivalent cation salt compositions

Publications (1)

Publication Number Publication Date
CY1123197T1 true CY1123197T1 (el) 2021-10-29

Family

ID=55911083

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20201100632T CY1123197T1 (el) 2015-04-23 2020-07-09 Μεθοδοι παρασκευης πολυνουκλεοτιδιου με χρηση χημικων συνθεσεων πολυσθενους αλατος κατιοντων

Country Status (36)

Country Link
US (2) US10745687B2 (enExample)
EP (1) EP3286203B1 (enExample)
JP (4) JP2018513127A (enExample)
KR (1) KR102401252B1 (enExample)
CN (2) CN107429247B (enExample)
AU (2) AU2016250576C1 (enExample)
BR (1) BR112017019627B1 (enExample)
CA (1) CA2978191C (enExample)
CL (1) CL2017002314A1 (enExample)
CO (1) CO2017009217A2 (enExample)
CY (1) CY1123197T1 (enExample)
DK (1) DK3286203T3 (enExample)
EA (1) EA035885B1 (enExample)
ES (1) ES2798270T3 (enExample)
HR (1) HRP20201218T1 (enExample)
HU (1) HUE051148T2 (enExample)
IL (1) IL254222A0 (enExample)
LT (1) LT3286203T (enExample)
MA (2) MA42569B1 (enExample)
MD (1) MD3286203T2 (enExample)
ME (1) ME03811B (enExample)
MX (1) MX383293B (enExample)
MY (1) MY187804A (enExample)
PE (2) PE20221275A1 (enExample)
PH (1) PH12017501927B1 (enExample)
PT (1) PT3286203T (enExample)
RS (1) RS60645B1 (enExample)
SA (1) SA517390103B1 (enExample)
SG (2) SG11201707893RA (enExample)
SI (1) SI3286203T1 (enExample)
SM (1) SMT202000389T1 (enExample)
TN (1) TN2017000411A1 (enExample)
TW (1) TWI736532B (enExample)
UA (1) UA124521C2 (enExample)
WO (1) WO2016172346A1 (enExample)
ZA (1) ZA201706041B (enExample)

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* Cited by examiner, † Cited by third party
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MA42569B1 (fr) 2015-04-23 2020-08-31 Geron Corp Procédés de préparation de polynucléotides à l'aide de compositions de sel cationique multivalent
US11384352B2 (en) 2016-12-13 2022-07-12 Modernatx, Inc. RNA affinity purification
EP4060345A1 (en) 2016-12-19 2022-09-21 Ventana Medical Systems, Inc. Peptide nucleic acid conjugates
US11866696B2 (en) * 2017-08-18 2024-01-09 Modernatx, Inc. Analytical HPLC methods
JP7441455B2 (ja) * 2017-09-22 2024-03-01 ザ リージェンツ オブ ザ ユニバーシティ オブ コロラド,ア ボディー コーポレイト 筋ジストロフィーの処置のためのチオモルホリノオリゴヌクレオチド
EP3727470B1 (en) 2017-12-18 2024-11-20 Ventana Medical Systems, Inc. Peptide nucleic acid conjugates

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Also Published As

Publication number Publication date
TW201706284A (zh) 2017-02-16
EA201791753A1 (ru) 2018-04-30
CA2978191C (en) 2022-10-04
TWI736532B (zh) 2021-08-21
HUE051148T2 (hu) 2021-03-01
CL2017002314A1 (es) 2018-05-04
SG10201909816PA (en) 2019-11-28
JP2025061848A (ja) 2025-04-11
US20200339975A1 (en) 2020-10-29
BR112017019627A2 (pt) 2018-05-15
AU2016250576B2 (en) 2021-02-04
UA124521C2 (uk) 2021-10-05
SA517390103B1 (ar) 2021-07-14
AU2016250576A1 (en) 2017-09-21
KR102401252B1 (ko) 2022-05-24
AU2021202803A1 (en) 2021-06-03
RS60645B1 (sr) 2020-09-30
CO2017009217A2 (es) 2018-02-20
PT3286203T (pt) 2020-07-08
ZA201706041B (en) 2020-11-25
BR112017019627B1 (pt) 2022-11-29
JP2021152079A (ja) 2021-09-30
CN113564168A (zh) 2021-10-29
AU2016250576C1 (en) 2021-05-06
NZ735042A (en) 2021-11-26
PH12017501927A1 (en) 2018-03-05
PE20171785A1 (es) 2017-12-27
CN107429247A (zh) 2017-12-01
SI3286203T1 (sl) 2020-10-30
WO2016172346A1 (en) 2016-10-27
IL254222A0 (en) 2017-10-31
MD3286203T2 (ro) 2020-08-31
MX383293B (es) 2025-03-13
PE20221275A1 (es) 2022-09-01
CN113564168B (zh) 2024-10-25
US11441144B2 (en) 2022-09-13
HK1251234A1 (en) 2019-01-25
PH12017501927B1 (en) 2022-09-02
KR20170138399A (ko) 2017-12-15
MY187804A (en) 2021-10-26
US10745687B2 (en) 2020-08-18
TN2017000411A1 (en) 2019-01-16
MA42157A (fr) 2018-02-28
SMT202000389T1 (it) 2020-09-10
SG11201707893RA (en) 2017-11-29
JP2018513127A (ja) 2018-05-24
EP3286203B1 (en) 2020-05-06
ES2798270T3 (es) 2020-12-10
EP3286203A1 (en) 2018-02-28
DK3286203T3 (da) 2020-06-02
HRP20201218T1 (hr) 2020-12-11
LT3286203T (lt) 2020-07-27
JP2022168017A (ja) 2022-11-04
EA035885B1 (ru) 2020-08-27
US20160312227A1 (en) 2016-10-27
MA42569B1 (fr) 2020-08-31
MX2017011642A (es) 2017-11-02
ME03811B (me) 2021-04-20
CA2978191A1 (en) 2016-10-27
CN107429247B (zh) 2021-08-13

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