KR102401252B1 - 다가 양이온 염 조성물을 사용한 폴리뉴클레오티드 제조의 방법 - Google Patents

다가 양이온 염 조성물을 사용한 폴리뉴클레오티드 제조의 방법 Download PDF

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KR102401252B1
KR102401252B1 KR1020177025835A KR20177025835A KR102401252B1 KR 102401252 B1 KR102401252 B1 KR 102401252B1 KR 1020177025835 A KR1020177025835 A KR 1020177025835A KR 20177025835 A KR20177025835 A KR 20177025835A KR 102401252 B1 KR102401252 B1 KR 102401252B1
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polynucleotide
salt
composition
counterion
polyvalent
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KR20170138399A (ko
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프렘찬드란 에이치. 라미야
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제론 코포레이션
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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/314—Phosphoramidates
    • C12N2310/3145—Phosphoramidates with the nitrogen in 3' or 5'-position
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3515—Lipophilic moiety, e.g. cholesterol
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    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase

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  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
KR1020177025835A 2015-04-23 2016-04-21 다가 양이온 염 조성물을 사용한 폴리뉴클레오티드 제조의 방법 Active KR102401252B1 (ko)

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US201562151891P 2015-04-23 2015-04-23
US62/151,891 2015-04-23
PCT/US2016/028657 WO2016172346A1 (en) 2015-04-23 2016-04-21 Methods of polynucleotide preparation using multivalent cation salt compositions

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KR102401252B1 true KR102401252B1 (ko) 2022-05-24

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MA42569B1 (fr) 2015-04-23 2020-08-31 Geron Corp Procédés de préparation de polynucléotides à l'aide de compositions de sel cationique multivalent
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JP7441455B2 (ja) * 2017-09-22 2024-03-01 ザ リージェンツ オブ ザ ユニバーシティ オブ コロラド，ア ボディー コーポレイト 筋ジストロフィーの処置のためのチオモルホリノオリゴヌクレオチド
EP3727470B1 (en) 2017-12-18 2024-11-20 Ventana Medical Systems, Inc. Peptide nucleic acid conjugates

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JP2007504830A (ja) * 2003-09-09 2007-03-08 ジェロン・コーポレーション テロメラーゼ阻害のための改変オリゴヌクレオチド

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EA201791753A1 (ru) 2018-04-30
CA2978191C (en) 2022-10-04
TWI736532B (zh) 2021-08-21
HUE051148T2 (hu) 2021-03-01
CL2017002314A1 (es) 2018-05-04
SG10201909816PA (en) 2019-11-28
JP2025061848A (ja) 2025-04-11
US20200339975A1 (en) 2020-10-29
BR112017019627A2 (pt) 2018-05-15
AU2016250576B2 (en) 2021-02-04
UA124521C2 (uk) 2021-10-05
SA517390103B1 (ar) 2021-07-14
AU2016250576A1 (en) 2017-09-21
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CY1123197T1 (el) 2021-10-29
RS60645B1 (sr) 2020-09-30
CO2017009217A2 (es) 2018-02-20
PT3286203T (pt) 2020-07-08
ZA201706041B (en) 2020-11-25
BR112017019627B1 (pt) 2022-11-29
JP2021152079A (ja) 2021-09-30
CN113564168A (zh) 2021-10-29
AU2016250576C1 (en) 2021-05-06
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PH12017501927A1 (en) 2018-03-05
PE20171785A1 (es) 2017-12-27
CN107429247A (zh) 2017-12-01
SI3286203T1 (sl) 2020-10-30
WO2016172346A1 (en) 2016-10-27
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US11441144B2 (en) 2022-09-13
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KR20170138399A (ko) 2017-12-15
MY187804A (en) 2021-10-26
US10745687B2 (en) 2020-08-18
TN2017000411A1 (en) 2019-01-16
MA42157A (fr) 2018-02-28
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JP2018513127A (ja) 2018-05-24
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