SG10201909816PA - Methods of polynucleotide preparation using multivalent cation salt compositions - Google Patents
Methods of polynucleotide preparation using multivalent cation salt compositionsInfo
- Publication number
- SG10201909816PA SG10201909816PA SG10201909816PA SG10201909816PA SG 10201909816P A SG10201909816P A SG 10201909816PA SG 10201909816P A SG10201909816P A SG 10201909816PA SG 10201909816P A SG10201909816P A SG 10201909816PA
- Authority
- SG
- Singapore
- Prior art keywords
- polynucleotide
- salt
- multivalent cation
- methods
- preparation
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
- C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/11—Antisense
- C12N2310/113—Antisense targeting other non-coding nucleic acids, e.g. antagomirs
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/31—Chemical structure of the backbone
- C12N2310/314—Phosphoramidates
- C12N2310/3145—Phosphoramidates with the nitrogen in 3' or 5'-position
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/35—Nature of the modification
- C12N2310/351—Conjugate
- C12N2310/3515—Lipophilic moiety, e.g. cholesterol
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase
Abstract
METHODS OF POLYNUCLEOTIDE PREPARATION USING MULTIVALENT CATION SALT COMPOSITIONS Aspects of the disclosure include methods for the preparation of a polynucleotide. In some embodiments, the method includes contacting a first polynucleotide composition including: a polynucleotide having a sequence of 7 or more nucleoside subunits and at least two of the nucleoside subunits are joined by a N3′→P5′ thiophosphoramidate inter-subunit linkage; and non-target synthetic products and reagents; with a multivalent cation salt to precipitate a polynucleotide salt including at least one multivalent cation counterion; and separating the polynucleotide salt from the contacted first polynucleotide composition to produce a second polynucleotide composition including the polynucleotide salt. In certain embodiments, the method further includes contacting the polynucleotide salt with a reverse phase chromatography support; and eluting from the chromatography support a third polynucleotide composition including the polynucleotide. Also provided are compositions including a salt of the polynucleotide including at least one multivalent cation counterion. FIG. 2
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562151891P | 2015-04-23 | 2015-04-23 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG10201909816PA true SG10201909816PA (en) | 2019-11-28 |
Family
ID=55911083
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG10201909816P SG10201909816PA (en) | 2015-04-23 | 2016-04-21 | Methods of polynucleotide preparation using multivalent cation salt compositions |
| SG11201707893RA SG11201707893RA (en) | 2015-04-23 | 2016-04-21 | Methods of polynucleotide preparation using multivalent cation salt compositions |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201707893RA SG11201707893RA (en) | 2015-04-23 | 2016-04-21 | Methods of polynucleotide preparation using multivalent cation salt compositions |
Country Status (36)
| Country | Link |
|---|---|
| US (2) | US10745687B2 (en) |
| EP (1) | EP3286203B1 (en) |
| JP (3) | JP2018513127A (en) |
| KR (1) | KR102401252B1 (en) |
| CN (2) | CN107429247B (en) |
| AU (2) | AU2016250576C1 (en) |
| BR (1) | BR112017019627B1 (en) |
| CA (1) | CA2978191C (en) |
| CL (1) | CL2017002314A1 (en) |
| CO (1) | CO2017009217A2 (en) |
| CY (1) | CY1123197T1 (en) |
| DK (1) | DK3286203T3 (en) |
| EA (1) | EA035885B1 (en) |
| ES (1) | ES2798270T3 (en) |
| HK (1) | HK1251234A1 (en) |
| HR (1) | HRP20201218T1 (en) |
| HU (1) | HUE051148T2 (en) |
| IL (1) | IL254222A0 (en) |
| LT (1) | LT3286203T (en) |
| MA (2) | MA42157A (en) |
| MD (1) | MD3286203T2 (en) |
| ME (1) | ME03811B (en) |
| MX (1) | MX2017011642A (en) |
| MY (1) | MY187804A (en) |
| PE (2) | PE20221275A1 (en) |
| PH (1) | PH12017501927A1 (en) |
| PT (1) | PT3286203T (en) |
| RS (1) | RS60645B1 (en) |
| SA (1) | SA517390103B1 (en) |
| SG (2) | SG10201909816PA (en) |
| SI (1) | SI3286203T1 (en) |
| TN (1) | TN2017000411A1 (en) |
| TW (1) | TWI736532B (en) |
| UA (1) | UA124521C2 (en) |
| WO (1) | WO2016172346A1 (en) |
| ZA (1) | ZA201706041B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SI3286203T1 (en) | 2015-04-23 | 2020-10-30 | Geron Corporation | Methods of polynucleotide preparation using multivalent cation salt compositions |
| US11384352B2 (en) | 2016-12-13 | 2022-07-12 | Modernatx, Inc. | RNA affinity purification |
| WO2018118759A1 (en) | 2016-12-19 | 2018-06-28 | Ventana Medical Systems, Inc. | Peptide nucleic acid conjugates |
| EP3668977A4 (en) * | 2017-08-18 | 2021-04-21 | Modernatx, Inc. | Analytical hplc methods |
| CN111542606A (en) * | 2017-09-22 | 2020-08-14 | 科罗拉多州立大学董事会法人团体 | Thiomorpholino oligonucleotides for treating muscular dystrophy |
| JP2021507695A (en) * | 2017-12-18 | 2021-02-25 | ヴェンタナ メディカル システムズ， インク． | Peptide nucleic acid conjugate |
Family Cites Families (35)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4046720A (en) | 1974-01-17 | 1977-09-06 | California Institute Of Technology | Crosslinked, porous, polyacrylate beads |
| US4413070A (en) | 1981-03-30 | 1983-11-01 | California Institute Of Technology | Polyacrolein microspheres |
| US4678814A (en) | 1981-03-30 | 1987-07-07 | California Institute Of Technology | Polyacrolein microspheres |
| US5185444A (en) | 1985-03-15 | 1993-02-09 | Anti-Gene Deveopment Group | Uncharged morpolino-based polymers having phosphorous containing chiral intersubunit linkages |
| US4659774A (en) | 1985-11-01 | 1987-04-21 | American Hoechst Corporation | Support for solid-phase oligonucleotide synthesis |
| DE69120821T2 (en) | 1990-08-31 | 1997-01-23 | Univ Minnesota | Polyethylene glycol derivatives for solid phase applications |
| US5281701A (en) | 1991-07-12 | 1994-01-25 | Applied Biosystems, Inc. | Process and compounds for RNA synthesis |
| US5476925A (en) | 1993-02-01 | 1995-12-19 | Northwestern University | Oligodeoxyribonucleotides including 3'-aminonucleoside-phosphoramidate linkages and terminal 3'-amino groups |
| US5726297A (en) | 1994-03-18 | 1998-03-10 | Lynx Therapeutics, Inc. | Oligodeoxyribonucleotide N3' P5' phosphoramidates |
| US5599922A (en) | 1994-03-18 | 1997-02-04 | Lynx Therapeutics, Inc. | Oligonucleotide N3'-P5' phosphoramidates: hybridization and nuclease resistance properties |
| AU704549B2 (en) | 1994-03-18 | 1999-04-29 | Lynx Therapeutics, Inc. | Oligonucleotide N3'-P5' phosphoramidates: synthesis and compounds; hybridization and nuclease resistance properties |
| US5684143A (en) | 1996-02-21 | 1997-11-04 | Lynx Therapeutics, Inc. | Oligo-2'-fluoronucleotide N3'->P5' phosphoramidates |
| US5859233A (en) | 1996-02-21 | 1999-01-12 | Lynx Therapeutics, Inc. | Synthons for synthesis of oligonucleotide N3-P5 phosphoramidates |
| EP0951568A1 (en) | 1996-12-20 | 1999-10-27 | Geron Corporation | Methods for detecting and inhibiting the rna component of telomerase |
| US5998604A (en) | 1997-09-15 | 1999-12-07 | The Perkin-Elmer Corporation | Polynucleotide purification method |
| US6114519A (en) | 1997-10-15 | 2000-09-05 | Isis Pharmaceuticals, Inc. | Synthesis of sulfurized oligonucleotides |
| US6465628B1 (en) | 1999-02-04 | 2002-10-15 | Isis Pharmaceuticals, Inc. | Process for the synthesis of oligomeric compounds |
| KR20070112295A (en) | 1999-09-10 | 2007-11-22 | 제론 코포레이션 | Oligonucleotide n3'→p5' thiophosphoramidates: their synthesis and use |
| JP2002060341A (en) * | 2000-08-21 | 2002-02-26 | Terumo Corp | Hemostatic agent |
| AU2002245717A1 (en) | 2001-03-23 | 2002-10-08 | Geron Corporation | Oligonucleotide conjugates |
| US7563618B2 (en) | 2001-03-23 | 2009-07-21 | Geron Corporation | Oligonucleotide conjugates |
| US20120329858A1 (en) * | 2010-08-04 | 2012-12-27 | Geron Corporation | Modified oligonucleotides for telomerase inhibition |
| KR101298493B1 (en) | 2003-09-09 | 2013-08-21 | 제론 코포레이션 | Modified oligonucleotides for telomerase inhibition |
| EP1783216A1 (en) | 2004-05-28 | 2007-05-09 | Sankyo Company, Limited | Telomerase-inhibitory ena oligonucleotide |
| HUE054463T2 (en) | 2004-07-02 | 2021-09-28 | Geron Corp | Synthesis of protected 3'-amino 5'-phosphoramidite nucleoside monomers |
| CA2624634A1 (en) * | 2005-10-03 | 2007-04-12 | Applera Corporation | Compositions, methods, and kits for amplifying nucleic acids |
| WO2008127305A2 (en) * | 2006-11-01 | 2008-10-23 | Biogen Idec Ma Inc. | Method of isolating biomacromolecules using low ph and divalent cations |
| EP2605794B1 (en) * | 2010-08-20 | 2016-07-20 | Replicor Inc. | Oligonucleotide chelate complexes |
| US9106103B2 (en) | 2011-09-23 | 2015-08-11 | Eaton Corporation | Unintteruptible power supply systems and methods employing on-demand energy storage |
| US9006630B2 (en) | 2012-01-13 | 2015-04-14 | Altasens, Inc. | Quality of optically black reference pixels in CMOS iSoCs |
| AR091065A1 (en) | 2012-05-18 | 2014-12-30 | Replicor Inc | A PHARMACEUTICAL FORMULATION THAT INCLUDES AN ANTIVIRAL OLIGONUCLEOTIDE CHELATE FOR THE TREATMENT OF AN ANTI-VIRAL INFECTION |
| CN110343673B (en) * | 2012-06-14 | 2024-04-05 | 生命技术公司 | Novel compositions, methods and kits for Polymerase Chain Reaction (PCR) |
| PT3456333T (en) | 2012-12-07 | 2020-06-01 | Geron Corp | Use of the telomerase inhibitor imetelstat for the treatment of myelodysplastic syndrome |
| JOP20200257A1 (en) | 2014-05-01 | 2017-06-16 | Geron Corp | Oligonucleotide Compositions and Methods of Making the Same |
| SI3286203T1 (en) | 2015-04-23 | 2020-10-30 | Geron Corporation | Methods of polynucleotide preparation using multivalent cation salt compositions |
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2016
- 2016-04-21 SI SI201630771T patent/SI3286203T1/en unknown
- 2016-04-21 EP EP16720636.6A patent/EP3286203B1/en active Active
- 2016-04-21 BR BR112017019627-1A patent/BR112017019627B1/en active IP Right Grant
- 2016-04-21 ES ES16720636T patent/ES2798270T3/en active Active
- 2016-04-21 MX MX2017011642A patent/MX2017011642A/en unknown
- 2016-04-21 PT PT167206366T patent/PT3286203T/en unknown
- 2016-04-21 SG SG10201909816P patent/SG10201909816PA/en unknown
- 2016-04-21 EA EA201791753A patent/EA035885B1/en unknown
- 2016-04-21 US US15/134,740 patent/US10745687B2/en active Active
- 2016-04-21 MA MA042157A patent/MA42157A/en unknown
- 2016-04-21 MY MYPI2017703910A patent/MY187804A/en unknown
- 2016-04-21 WO PCT/US2016/028657 patent/WO2016172346A1/en active Application Filing
- 2016-04-21 HU HUE16720636A patent/HUE051148T2/en unknown
- 2016-04-21 PE PE2022001164A patent/PE20221275A1/en unknown
- 2016-04-21 PE PE2017001516A patent/PE20171785A1/en unknown
- 2016-04-21 CA CA2978191A patent/CA2978191C/en active Active
- 2016-04-21 KR KR1020177025835A patent/KR102401252B1/en active IP Right Grant
- 2016-04-21 CN CN201680014382.0A patent/CN107429247B/en active Active
- 2016-04-21 MA MA42569A patent/MA42569B1/en unknown
- 2016-04-21 ME MEP-2020-161A patent/ME03811B/en unknown
- 2016-04-21 CN CN202110878917.1A patent/CN113564168A/en active Pending
- 2016-04-21 SG SG11201707893RA patent/SG11201707893RA/en unknown
- 2016-04-21 TW TW105112455A patent/TWI736532B/en active
- 2016-04-21 LT LTEP16720636.6T patent/LT3286203T/en unknown
- 2016-04-21 TN TNP/2017/000411A patent/TN2017000411A1/en unknown
- 2016-04-21 RS RS20200935A patent/RS60645B1/en unknown
- 2016-04-21 MD MDE20180204T patent/MD3286203T2/en unknown
- 2016-04-21 UA UAA201708890A patent/UA124521C2/en unknown
- 2016-04-21 AU AU2016250576A patent/AU2016250576C1/en active Active
- 2016-04-21 DK DK16720636.6T patent/DK3286203T3/en active
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2017
- 2017-08-30 IL IL254222A patent/IL254222A0/en active IP Right Grant
- 2017-09-05 ZA ZA201706041A patent/ZA201706041B/en unknown
- 2017-09-12 CO CONC2017/0009217A patent/CO2017009217A2/en unknown
- 2017-09-13 CL CL2017002314A patent/CL2017002314A1/en unknown
- 2017-10-04 SA SA517390103A patent/SA517390103B1/en unknown
- 2017-10-20 PH PH12017501927A patent/PH12017501927A1/en unknown
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2018
- 2018-08-18 HK HK18110626.1A patent/HK1251234A1/en unknown
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2020
- 2020-07-09 CY CY20201100632T patent/CY1123197T1/en unknown
- 2020-07-10 US US16/926,340 patent/US11441144B2/en active Active
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2021
- 2021-05-04 AU AU2021202803A patent/AU2021202803A1/en not_active Abandoned
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2022
- 2022-09-02 JP JP2022140111A patent/JP2022168017A/en active Pending
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