CN1660913A - 抗hcv抗体的免疫测定 - Google Patents
抗hcv抗体的免疫测定 Download PDFInfo
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- CN1660913A CN1660913A CN2004100116606A CN200410011660A CN1660913A CN 1660913 A CN1660913 A CN 1660913A CN 2004100116606 A CN2004100116606 A CN 2004100116606A CN 200410011660 A CN200410011660 A CN 200410011660A CN 1660913 A CN1660913 A CN 1660913A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/576—Immunoassay; Biospecific binding assay; Materials therefor for hepatitis
- G01N33/5767—Immunoassay; Biospecific binding assay; Materials therefor for hepatitis non-A, non-B hepatitis
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/24011—Flaviviridae
- C12N2770/24211—Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
- C12N2770/24222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
- G01N2333/08—RNA viruses
- G01N2333/18—Togaviridae; Flaviviridae
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2469/00—Immunoassays for the detection of microorganisms
- G01N2469/10—Detection of antigens from microorganism in sample from host
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2469/00—Immunoassays for the detection of microorganisms
- G01N2469/20—Detection of antibodies in sample from host which are directed against antigens from microorganisms
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Investigating Or Analyzing Materials By The Use Of Magnetic Means (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Plural Heterocyclic Compounds (AREA)
Abstract
Description
表1 | |
结构域 | 大约的边界* |
C(核心) | 1-191 |
E1 | 192-383 |
E2 | 384-746 |
P7 | 747-809 |
NS2 | 810-1026 |
NS3 | 1027-1657 |
NS4a | 1658-1711 |
NS4b | 1712-1972 |
NS5a | 1973-2420 |
NS5b | 2421-3011 |
表2 MEFA7.1 | ||||
mefa aa# | 5′末端位点 | 表位 | hcv aa# | 菌株 |
1-156 | Nco1 | hSOD | ||
159-176 | EcoR1 | E1 | 303-320 | 1 |
179-199 | Hind111 | E2 HVR1a共有序列 | 390-410 | 1 |
200-230 | E2 HVR1+2共有序列 | 384-414 | 1+2 | |
231-696 | Sal1 | 解旋酶 | 1193-1658 | 1 |
699-745 | sph1 | 5-1-1 | 1689-1735 | 1 |
748-794 | Nru1 | 5-1-1 | 1689-1735 | 3 |
797-843 | Cla1 | 5-1-1 | 1689-1735 | 2 |
846-881 | Ava1 | C100 | 1901-1936 | 1 |
884-919 | Xba1 | NS5 | 2278-2313 | 1 |
922-957 | Bgl11 | NS5 | 2278-2313 | 1 |
958-1028 | Nco1 | 核心表位 | 9-32,39-4264-8867-84 | 112 |
1029-1099 | Bal1 | 核心表位 | 9-32,39-42,64-8867-84 | 112 |
实验组 | 出血天数早期检测 | 早期c33c实验组 | 早期c22实验组 | |||
HCV3.0 | Abbott Prism | |||||
#1#2#3#4#5#6#7#8#9#10#11#12#13#14#15#16#17 | c33cc33cc22c33cc22c22c22c22c22c33cc33cc33cc33cc33cc22c22c33c | PHV904PHV905PHV907PHV908PHV909PHV910PHV911PHV912PHV913PHV9146212621362146222SC-0010SC-0030SC-0040 | 2738000021214674059 | 2300000021212004057 | PHV904PHV905PHV908PHV9146212621362146222SC-0040 | PHV907PHV909PHV910PHV911PHV912PHV913SC-0010SC-0030 |
改善范围 | 2-14天 | 2-12天 |
稀释 | 基因型 | HCV4.0原型s | HCV3.0Orthos | Monolisa 2版Pasteurs | 基因型 | HCV4.0原型s | HCV3.0Orthos | Monolisa 2版Pasteurs | |
1∶25001∶50001∶100001∶200001∶25001∶50001∶100001∶200001∶25001∶50001∶100001∶200001∶25001∶50001∶100001∶200001∶25001∶50001∶100001∶20000 | 1b2b2a/c3a3e | 2.8662.0741.0990.4031.4300.5510.2250.0741.9520.9170.3950.1082.5801.6220.8730.3981.2070.4610.1550.054 | 1.0070.3930.1590.0450.2950.1080.0320.0100.4670.1360.0490.0110.5140.2180.0670.0230.1580.0390.0110.003 | 0.6940.2180.0840.0280.6580.2070.0610.0191.6530.7820.2860.1050.9410.3530.1640.0500.2910.1140.0530.024 | 1∶101∶1001∶10001∶10000 | 4b/c4a4c5a6 | 2.4781.1250.6090.2162.8311.7520.7170.2481.7510.8560.3840.1412.6822.7441.5870.7263.5163.6023.2241.192 | 0.7010.2560.0870.0350.6320.1930.0690.0150.4570.1690.0550.0181.4960.8270.3160.0973.2473.5942.8630.380 | 0.5510.1950.0760.0330.4620.1810.0760.0251.1470.4740.1780.0582.2710.9880.3950.120NDNDNDND |
1 | 2 | 3 | 4 | |
c200 | + | PHV914-5 | 对照1×PBS | %抑制 |
s | s | |||
1微克 | 1.450 | 1.645 | 12 | |
1微克 | 1.545 | 1.687 | 8 | |
0.5微克 | 1.557 | 1.913 | 19 | |
0.5微克 | 1.719 | 1.804 | 5 | |
NS3/4a | + | PHV914-5 | ||
s | s | |||
1微克 | 0.054 | 1.599 | 97 | |
1微克 | 0.037 | 1.677 | 98 | |
0.5微克 | 0.066 | 1.672 | 96 | |
0.5微克 | NA | 1.524 | NA |
时间(hr) | 0 | 6 | 21.4 | 29 | 35.5 | 46 | 52 | 对照 |
A | D | G | H | I | K | N | 参照 | |
s/co | s/co | s/co | s/co | s/co | s/co | s/co | s/co | |
PHV904-1 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
PHV904-2 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
PHV904-3 | 1.5 | 0.3 | 0.1 | 0.0 | 0.0 | 0.0 | 0.0 | 1.8 |
PHV904-4 | 3.7 | 1.0 | 0.2 | 0.1 | 0.1 | 0.1 | 0.1 | 4.4 |
PHV904-5 | 4.8 | 2.0 | 0.7 | 0.6 | 0.3 | 0.2 | 0.3 | 5.5 |
PHV904-6 | 5.4 | 2.8 | 1.1 | 1.0 | 0.6 | 0.5 | 0.6 | 5.8 |
PHV904-7 | 5.1 | 3.4 | 1.5 | 1.0 | 1.1 | 0.5 | 0.7 | 5.4 |
PHV914-1 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
PHV914-2 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
PHV914-3 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
PHV914-4 | 0.5 | 0.1 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.7 |
PHV914-5 | 2.1 | 0.4 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 3.0 |
PHV914-6 | 2.3 | 0.4 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 3.4 |
PHV914-7 | 2.8 | 0.5 | 0.1 | 0.1 | 0.0 | 0.0 | 0.0 | 4.9 |
PHV914-8 | 2.9 | 0.7 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 4.9 |
酶 |
RFU/分钟 | 8.74 | 4.14 | 3.08 | 1.88 | 1.75 | 1.75 | 0.75 |
NS3/4a对变性的NS3/4a | ||||||||
*储存液NS3/4a掺入2%SDS | ||||||||
NS3/4a | dNS3/4a | c200 | NS3/4a | dNS3/4a | c200 | |||
OD | OD | OD | s/co | s/co | s/co | |||
HCV | PHV904-1 | 0.012 | 0.012 | 0.009 | 0.02 | 0.02 | 0.01 | |
血清转化 | PHV904-2 | 0.011 | 0.009 | 0.008 | 0.02 | 0.01 | 0.01 | |
PHV904-3 | 1.124 | 0.071 | 0.045 | 1.80 | 0.11 | 0.07 | ||
PHV904-4 | 2.401 | 0.273 | 0.129 | 3.85 | 0.44 | 0.21 | ||
PHV904-5 | 3.022 | 0.793 | 0.347 | 4.85 | 1.28 | 0.57 | ||
PHV904-6 | 2.711 | 1.472 | 0.774 | 4.35 | 2.37 | 1.28 | ||
PHV904-7 | 3.294 | 1.860 | 0.943 | 5.28 | 2.99 | 1.55 | ||
PHV914-1 | 0.006 | 0.004 | 0.001 | 0.01 | 0.01 | 0.00 | ||
PHV914-2 | 0.005 | 0.004 | 0.002 | 0.01 | 0.01 | 0.00 |
PHV914-3 | 0.098 | 0.003 | 0.001 | 0.16 | 0.00 | 0.00 | ||
PHV914-4 | 1.118 | 0.006 | 0.004 | 1.79 | 0.01 | 0.01 | ||
PHV914-5 | 2.035 | 0.044 | 0.022 | 3.26 | 0.07 | 0.04 | ||
PHV914-6 | 2.092 | 0.074 | 0.025 | 3.35 | 0.12 | 0.04 | ||
PHV914-7 | 2.519 | 0.281 | 0.132 | 4.04 | 0.45 | 0.22 | ||
PHV914-8 | 2.746 | 0.907 | 0.500 | 4.40 | 1.46 | 0.82 | ||
PHV914-9 | 3.084 | 1.730 | 0.931 | 4.94 | 2.78 | 1.53 | ||
HCV3.0 | 阴性对照 | 0.023 | 0.024 | 0.008 | ||||
对照 | 阴性对照 | 0.027 | 0.024 | 0.007 | ||||
阴性对照 | 0.021 | 0.017 | 0.005 | |||||
平均 | 0.024 | 0.022 | 0.007 | |||||
截留 | 0.624 | 0.622 | 0.607 | |||||
阳性对照 | 1.239 | 0.903 | 0.575 | 1.99 | 1.45 | 0.95 | ||
阳性对照 | 1.445 | 0.916 | 0.614 | 2.32 | 1.47 | 1.01 |
表9 | ||||
平板NS3/4a dNS3/4a c200 | ||||
单克隆 | OD | OD | OD | |
4B9/E3 | 1∶100 | 1.820 | 0.616 | 0.369 |
1∶1000 | 1.397 | 0.380 | 0.246 | |
1∶10000 | 0.864 | 0.173 | 0.070 |
1∶20000 | 0.607 | 0.116 | 0.085 | |
5B7/D7 | 1∶100 | 2.885 | 0.898 | 0.436 |
1∶1000 | 2.866 | 0.541 | 0.267 | |
1∶10000 | 1.672 | 0.215 | 0.086 | |
1∶20000 | 1.053 | 0.124 | 0.059 | |
1A8/H2 | 1∶100 | 1.020 | 0.169 | 0.080 |
1∶1000 | 0.921 | 0.101 | 0.043 | |
1∶10000 | 0.653 | 0.037 | 0.013 | |
1∶20000 | 0.337 | 0.027 | 0.011 |
Claims (11)
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US21208200P | 2000-06-15 | 2000-06-15 | |
US60/212,082 | 2000-06-15 | ||
US28086701P | 2001-04-02 | 2001-04-02 | |
US28081101P | 2001-04-02 | 2001-04-02 | |
US60/280,867 | 2001-04-02 | ||
US60/280,811 | 2001-04-02 |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB018112463A Division CN1214244C (zh) | 2000-06-15 | 2001-06-14 | 免疫测定固相支持物、其试剂盒 、制备以及用途 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1660913A true CN1660913A (zh) | 2005-08-31 |
CN100463922C CN100463922C (zh) | 2009-02-25 |
Family
ID=27395684
Family Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB2004100116606A Expired - Lifetime CN100463922C (zh) | 2000-06-15 | 2001-06-14 | 抗hcv抗体的免疫测定 |
CNB018112463A Expired - Lifetime CN1214244C (zh) | 2000-06-15 | 2001-06-14 | 免疫测定固相支持物、其试剂盒 、制备以及用途 |
CNB018112439A Expired - Lifetime CN1256591C (zh) | 2000-06-15 | 2001-06-14 | Hcv抗原/抗体组合试验 |
Family Applications After (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB018112463A Expired - Lifetime CN1214244C (zh) | 2000-06-15 | 2001-06-14 | 免疫测定固相支持物、其试剂盒 、制备以及用途 |
CNB018112439A Expired - Lifetime CN1256591C (zh) | 2000-06-15 | 2001-06-14 | Hcv抗原/抗体组合试验 |
Country Status (23)
Country | Link |
---|---|
US (5) | US6632601B2 (zh) |
EP (2) | EP1354204B2 (zh) |
JP (3) | JP4834279B2 (zh) |
CN (3) | CN100463922C (zh) |
AT (2) | ATE368221T1 (zh) |
AU (2) | AU2001272945A1 (zh) |
BG (1) | BG66205B1 (zh) |
BR (2) | BRPI0111682B8 (zh) |
CA (2) | CA2412035C (zh) |
CY (2) | CY1107537T1 (zh) |
CZ (1) | CZ304185B6 (zh) |
DE (2) | DE60129598T2 (zh) |
DK (2) | DK1350105T3 (zh) |
ES (2) | ES2277932T5 (zh) |
HK (2) | HK1061861A1 (zh) |
HU (1) | HU228873B1 (zh) |
MX (2) | MXPA02012401A (zh) |
NO (1) | NO332275B1 (zh) |
PL (1) | PL213363B1 (zh) |
PT (2) | PT1354204E (zh) |
SI (1) | SI1354204T2 (zh) |
SK (1) | SK287694B6 (zh) |
WO (2) | WO2001096870A2 (zh) |
Families Citing this family (40)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE69433160T2 (de) * | 1993-05-12 | 2004-07-08 | Chiron Corp. (N.D.Ges.D. Staates Delaware), Emeryville | Konserviertes Motiv der Hepatitis C Virus E2/NS1 Region |
AU2001272945A1 (en) * | 2000-06-15 | 2001-12-24 | Chiron Corporation | Immunoassays for anti-hcv antibodies |
US7491808B2 (en) * | 2000-06-15 | 2009-02-17 | Novartis Vaccines And Diagnostics, Inc. | HCV non-structural protein mutants and uses thereof |
WO2002014362A2 (en) * | 2000-08-17 | 2002-02-21 | Tripep Ab | A hepatitis c virus non-structural ns3/4a fusion gene |
US7022830B2 (en) | 2000-08-17 | 2006-04-04 | Tripep Ab | Hepatitis C virus codon optimized non-structural NS3/4A fusion gene |
DE10106295C1 (de) * | 2001-02-02 | 2002-08-22 | Gaifar German American Inst Fo | Protein mit mehreren Antigen-Epitop-Sequenzen, welches immobilisiert ist |
SG118120A1 (en) * | 2001-03-28 | 2006-01-27 | Ortho Clinical Diagnostics Inc | A hepatitis C antigen - antibody combination assayfor the early detection of HCV infection |
US7101683B2 (en) | 2001-06-26 | 2006-09-05 | Abbott Laboratories | Methods for the simultaneous detection of HCV antigens and HCV antibodies |
EP1412538B1 (en) * | 2001-06-26 | 2013-03-27 | Abbott Laboratories | Methods for the simultaneous detection of hcv antigens and hcv antibodies |
US7049060B2 (en) * | 2001-11-05 | 2006-05-23 | Ortho-Clinical Diagnostics, Inc. | HCV anti-core monoclonal antibodies |
US7332269B2 (en) * | 2001-11-11 | 2008-02-19 | Ortho-Clinical Diagnostics, Inc. | HCV core protein sequences |
FR2839555B1 (fr) * | 2002-05-10 | 2007-07-27 | Bio Rad Pasteur | Procede de detection simultanee d'un antigene et d'un anticorps d'un microorganisme infectieux |
EP1546414B1 (en) * | 2002-09-09 | 2008-10-08 | Novartis Vaccines and Diagnostics, Inc. | Hcv assay |
US20040152070A1 (en) * | 2003-02-04 | 2004-08-05 | Shah Dinesh O. | Method of detection of HCV antibodies in combination assay or sole antibody assay |
US20070003977A1 (en) | 2003-08-20 | 2007-01-04 | Amorfix Life Sciences Ltd | Epitope protection assay and method for detecting protein conformations |
WO2006024020A2 (en) | 2004-08-27 | 2006-03-02 | Novartis Vaccines And Diagnostics Inc. | Hcv non-structural protein mutants and uses thereof |
US7658930B2 (en) * | 2005-02-02 | 2010-02-09 | Peng Cui | Kit for detecting the antibody of HCV and its preparing method |
CA2630220C (en) | 2005-11-22 | 2020-10-13 | Doris Coit | Norovirus and sapovirus antigens |
US7794692B2 (en) * | 2005-12-02 | 2010-09-14 | Amorfix Life Sciences Ltd. | Methods and compositions for detecting amyotrophic lateral sclerosis |
US7887803B2 (en) | 2005-12-02 | 2011-02-15 | Amorfix Life Sciences | Methods and compositions to treat misfolded-SOD1 mediated diseases |
EP2514823B1 (en) | 2006-03-03 | 2018-05-02 | ProMIS Neurosciences Inc. | Methods and compositions to treat and detect misfolded-SOD1 mediated diseases |
CN1908666B (zh) * | 2006-08-14 | 2010-05-12 | 武汉大学 | 一种检测乙肝核心抗体的双夹心法酶联免疫诊断试剂盒及应用 |
US8071561B2 (en) | 2007-08-16 | 2011-12-06 | Chrontech Pharma Ab | Immunogen platform |
CN102378914B (zh) * | 2009-03-30 | 2014-12-10 | 生物梅里埃公司 | 用于hcv检测的固体支持物 |
US20100297607A1 (en) * | 2009-05-20 | 2010-11-25 | Jian Zheng | Reagents For HCV Antigen-Antibody Combination Assays |
JP2013529894A (ja) | 2010-04-07 | 2013-07-25 | ノバルティス アーゲー | パルボウイルスb19のウイルス様粒子を生成するための方法 |
CN106771219B (zh) * | 2010-06-17 | 2020-04-07 | 皇家飞利浦电子股份有限公司 | 多表位测定 |
CA2804501C (en) | 2010-07-06 | 2021-01-26 | Novartis Ag | Noro virus derived immunogenic compositions and methods |
WO2012006500A2 (en) * | 2010-07-08 | 2012-01-12 | Abbott Laboratories | Monoclonal antibodies against hepatitis c virus core protein |
CA2824758C (en) * | 2011-01-13 | 2019-06-04 | Ortho-Clinical Diagnostics, Inc. | Treponema pallidum triplet antigen |
FR2984328B1 (fr) | 2011-12-20 | 2016-12-30 | Bio-Rad Innovations | Procede de detection d'une infection par le virus de l'hepatite c |
US9371374B2 (en) | 2013-03-14 | 2016-06-21 | Abbott Laboratories | HCV core lipid binding domain monoclonal antibodies |
CN105228649B (zh) | 2013-03-14 | 2019-01-18 | 雅培制药有限公司 | Hcv抗原-抗体组合测定和方法以及用在其中的组合物 |
MX2015012824A (es) | 2013-03-14 | 2016-06-24 | Abbott Lab | Antigenos recombinantes ns3 del vhc y mutantes de los mismos para la deteccion mejorada de anticuerpos. |
CN103630690B (zh) * | 2013-12-17 | 2014-08-20 | 朱之炜 | 丙型肝炎病毒抗原抗体联合检测试剂盒及其检测方法 |
WO2016160046A1 (en) | 2015-03-27 | 2016-10-06 | Ortho-Clinical Diagnostics, Inc. | Hcv ns4a/modified ns3 polypeptides and uses thereof |
US20180292408A1 (en) * | 2015-04-20 | 2018-10-11 | Qoolabs, Inc. | Camelid single-domain hcv antibodies and methods of use |
US11639933B2 (en) * | 2017-09-27 | 2023-05-02 | Bio-Rad Laboratories, Inc. | Digital affinity linkage assay |
CN110261616B (zh) * | 2019-04-30 | 2021-07-20 | 广东菲鹏生物有限公司 | 一种丙型肝炎病毒检测试剂盒 |
CN115838420B (zh) * | 2022-10-31 | 2023-05-05 | 北京科跃中楷生物技术有限公司 | 一种可溶性hcv重组蛋白的制备方法及其制备的抗体检测试剂 |
Family Cites Families (27)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6171782B1 (en) | 1987-11-18 | 2001-01-09 | Chiron Corporation | Antibody compositions to HCV and uses thereof |
EP0318216B2 (en) * | 1987-11-18 | 2001-08-29 | Chiron Corporation | NANBV diagnostics |
US5683864A (en) * | 1987-11-18 | 1997-11-04 | Chiron Corporation | Combinations of hepatitis C virus (HCV) antigens for use in immunoassays for anti-HCV antibodies |
YU48038B (sh) * | 1987-11-18 | 1996-10-18 | Chiron Corp. | Postupak za dijagnostiku ne-a, ne-v hepatitisa |
US5350671A (en) | 1987-11-18 | 1994-09-27 | Chiron Corporation | HCV immunoassays employing C domain antigens |
JP2730153B2 (ja) | 1989-03-16 | 1998-03-25 | 日本合成ゴム株式会社 | 熱可塑性樹脂組成物およびその製造方法 |
HUT54896A (en) | 1989-03-17 | 1991-04-29 | Chiron Corp | Process for producing aquous diagnosticum and vaccine of nanbv |
GEP20002164B (en) * | 1989-05-18 | 2000-07-10 | Novartis Vaccines & Diagnostic | Oligomers, method for detecting of hcv sequence, kit for its detecting, method for production of the blood free from hcv |
US5712087A (en) | 1990-04-04 | 1998-01-27 | Chiron Corporation | Immunoassays for anti-HCV antibodies employing combinations of hepatitis C virus (HCV) antigens |
US6194140B1 (en) * | 1990-04-04 | 2001-02-27 | Chiron Corporation | HCV NS3 protein fragments having helicase activity and improved solubility |
JP2733138B2 (ja) * | 1990-04-04 | 1998-03-30 | カイロン コーポレイション | 抗hcv抗体の免疫アッセイに使用するc型肝炎ウイルス(hcv)抗原の組合せ |
CA2049679C (en) * | 1990-08-24 | 2005-06-21 | Sushil G. Devare | Hepatitis c assay utilizing recombinant antigens |
PT97247B (pt) * | 1991-04-03 | 1997-10-31 | Chiron Corp | Processo de preparacao de combinacoes de antigenios do virus da hepapite c (hcv) para uso em imunoensaios para anticorpos anti-hcv |
DE69232871T2 (de) | 1991-06-24 | 2003-05-08 | Chiron Corp | Polypeptide des hepatitis c-virus (hiv) |
UA39944C2 (uk) | 1992-07-07 | 2001-07-16 | Чірон Корпорейшн | Спосіб визначення ранньої сероконверсії у ссавця-хазяїна до вірусу гепатиту с і набір для використання в способі |
EP1004670B1 (en) * | 1993-04-27 | 2012-04-11 | Innogenetics N.V. | Sequences of hepatitis C virus genotypes and their use as therapeutic and diagnostic agents |
US6001604A (en) * | 1993-12-29 | 1999-12-14 | Bio-Technology General Corp. | Refolding of proinsulins without addition of reducing agents |
JP3217600B2 (ja) | 1994-07-12 | 2001-10-09 | 株式会社先端生命科学研究所 | 非a非b型肝炎ウイルス関連抗原のイムノアッセイ、それに使用するモノクローナル抗体、およびこの抗体を産生するハイブリドーマ |
DE4428705A1 (de) * | 1994-08-12 | 1996-02-15 | Boehringer Mannheim Gmbh | Rekombinantes Antigen aus der NS3-Region des Hepatitis C Virus |
US5843752A (en) | 1995-05-12 | 1998-12-01 | Schering Corporation | Soluble active hepatitis C virus protease |
US5990276A (en) | 1996-05-10 | 1999-11-23 | Schering Corporation | Synthetic inhibitors of hepatitis C virus NS3 protease |
AU719929B2 (en) | 1996-05-24 | 2000-05-18 | Grifols Worldwide Operations Limited | Multiple epitope fusion protein |
US6514731B1 (en) * | 1996-05-24 | 2003-02-04 | Chiron Corporation | Methods for the preparation of hepatitis C virus multiple copy epitope fusion antigens |
EP0870830A3 (en) * | 1997-02-10 | 2004-02-25 | Advanced Life Science Institute, Inc. | Chimera hepatitis C virus antigen |
DE69837155T2 (de) * | 1997-09-22 | 2007-06-21 | Novartis Vaccines and Diagnostics, Inc., Emeryville | Verfahren zum nachweiss von antikörpern in einer probe |
BR9906660A (pt) * | 1998-07-30 | 2000-08-29 | Advanced Life Science Inst Inc | Processo para medir vìrus da hepatite c |
AU2001272945A1 (en) * | 2000-06-15 | 2001-12-24 | Chiron Corporation | Immunoassays for anti-hcv antibodies |
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