CN1620330A - 从含油种子和微生物来源提取和冬化脂类的方法 - Google Patents
从含油种子和微生物来源提取和冬化脂类的方法 Download PDFInfo
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- CN1620330A CN1620330A CNA028280431A CN02828043A CN1620330A CN 1620330 A CN1620330 A CN 1620330A CN A028280431 A CNA028280431 A CN A028280431A CN 02828043 A CN02828043 A CN 02828043A CN 1620330 A CN1620330 A CN 1620330A
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Classifications
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/006—Refining fats or fatty oils by extraction
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B7/00—Separation of mixtures of fats or fatty oils into their constituents, e.g. saturated oils from unsaturated oils
- C11B7/0008—Separation of mixtures of fats or fatty oils into their constituents, e.g. saturated oils from unsaturated oils by differences of solubilities, e.g. by extraction, by separation from a solution by means of anti-solvents
- C11B7/0025—Separation of mixtures of fats or fatty oils into their constituents, e.g. saturated oils from unsaturated oils by differences of solubilities, e.g. by extraction, by separation from a solution by means of anti-solvents in solvents containing oxygen in their molecule
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Fats And Perfumes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Extraction Or Liquid Replacement (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Edible Oils And Fats (AREA)
Abstract
Description
FA名称 | 未冬化的样品1 | 分离的固体级分 | 分离的液体级分样品1 | 样品2沉降物 | 分离的固体级分样品2沉降物 | 分离的液体级分批次21A沉降物 |
14:0 | 9.6 | 25.9 | 8.3 | 12.2 | 27.0 | 8.4 |
16:0 | 25.9 | 66.0 | 23.1 | 30.5 | 58.8 | 23.2 |
16:1 | 0.3 | <0.1 | 0.3 | 0.3 | 0.2 | 0.3 |
18:0 | 0.7 | 1.8 | 0.6 | 0.7 | 1.5 | 0.6 |
18:4n3 | 0.4 | <0.1 | 0.4 | 0.3 | <0.1 | 0.4 |
20:3n6 | 0.4 | 0.2 | 0.4 | 0.3 | 0.2 | 0.5 |
20:4n7 | 2.8 | <0.1 | 2.6 | 1.8 | <0.1 | 2.4 |
20:4n6 | 0.9 | <0.1 | 1.0 | 0.8 | 0.1 | 1.0 |
20:4n3 | 0.8 | <0.1 | 0.9 | 0.8 | <0.1 | 0.9 |
20:5n3 | 2.2 | <0.1 | 2.3 | 1.9 | 0.3 | 2.3 |
22:4n9 | 0.2 | <0.1 | 0.1 | 0.2 | <0.1 | 0.2 |
22:5n6 | 14.7 | 0.9 | 16.0 | 13.6 | 2.6 | 16.3 |
22:6n3 | 37.7 | 2.4 | 41.0 | 34.2 | 6.4 | 41.1 |
规格 | 结果 | 方法参考 | |
过氧化物值,meq/kg | 最大值3.0 | 0.42 | AOCS Cd 8-53 |
游离脂肪酸,% | 最大值0.25 | 0.06 | AOCS Ca 5a-40 |
水分和挥发性物质,% | 最大值0.05 | 0.03 | AOCS Ca 2d-25 |
痕量金属,ppm | POS AS.SOP-103 | ||
铅 | 最大值0.20 | <0.20 | |
砷 | 最大值0.20 | <0.20 | |
铁 | 最大值0.20 | 0.04 | |
铜 | 最大值0.05 | <0.05 | |
汞 | 最大值0.20 | <0.20 | |
DHA,%of FAME,wt/wt | 最小值32.0 | 43.5 | POS AS.SOP-104 |
DHA,mg/g油 | 最小值300 | 397.3 | POS AS.SOP-104 |
残余己烷,ppm | 最大值10 | <1.0 | AOCS Ca 3b-87 |
规格 | 值 | 方法参考 | |
中性油,% | N/A | 99.69 | |
对茴香胶值 | N/A | 0.74 | AOCS Cd 18-90 |
颜色,1.0″Lovibond(PFX 990 AOCS) | N/A | 70.0Y 7.1R | AutoTintometerColour |
颜色,Gardner级,(1cm) | N/A | 12.3 | |
β-胡萝卜素(PFX990),ppm,(0.01cm)注意:nottrueβ-胡萝卜素 | N/A | 276.41 |
不皂化,% | N/A | 2.24 | AOCS Ca 6b-53 |
不溶性杂质,% | N/A | 0.01 | AOCS Ca 3-46 |
AOM,小时 | N/A | 7.66 | AOCS Cd 12-57 |
Rancimat(80℃),小时 | N/A | 22.7 | |
自旋试验,按体积计的固体%,20℃/加入抗氧化剂后24小时 | N/A | ~0.2* | |
自旋试验,按体积计的固体%,加入抗氧化剂前 | N/A | 0 | |
脂肪酸组成(绝对值),mg/g | N/A | POSAS.SOP-104 | |
C12 | 2.6 | ||
C14 | 69.4 | ||
C14:1 | 0.8 | ||
C15 | 3.1 | ||
C16 | 187.8 | ||
C16:1 | 4.4 | ||
C18 | 4.6 | ||
C18:1 | 7.2 | ||
C18:2 | 3.6 | ||
C18:3n6 | 2.3 | ||
C18:4 | 3.0 | ||
C20 | 1.2 | ||
C20:4n6 | 7.4 | ||
C20:4n3 AA | 8.5 | ||
C20:5n3 EPA | 18.2 | ||
C22 | 0.6 | ||
C22:5n6+DPA | 151.6 | ||
C22:6n3 DHA | 397.3 | ||
C24 | 1.8 | ||
C24:1 | 1.9 |
其它 | 35.1 | ||
总计,mg/g | 912.4 | ||
DHA,FAME的% | 43.5 | ||
棕榈酸抗坏血酸酯,ppm | 224 | ||
生育酚类,ppm | 1,760 |
规格 | 结果 | 方法参考 | |
-过氧化物值,meq/kg | 最大值3.0 | 1.32 | AOCS Cd 8-53 |
游离脂肪酸,% | 最大值0.25 | 0.06 | AOCS Ca 5a-40 |
水分和挥发性物质,% | 最大值0.05 | 0.03 | AOCS Ca 2d-25 |
痕量金属,ppm | POS AS.SOP-103 | ||
铅 | 最大值0.20 | <0.20 | |
由 | 最大值0.20 | <0.20 | |
铁 | 最大值0.20 | 0.11 | |
铜 | 最大值0.05 | <0.05 | |
汞 | 最大值0.20 | <0.20 | |
DHA,FAME的% | 最小值32.0 | 42.8 | POS AS.SOP-104 |
DHA,mg/g油 | 最小值300 | 385.5 | POS AS.SOP-104 |
残余的己烷,ppm | 最小值10 | <1.0 | AOCS Ca 3b-87 |
规格 | 值 | 方法参考 | |
中性油,% | N/A | 99.69 | |
对茴香胺值 | N/A | 1.08 | AOCS Cd 18-90 |
颜色,1.0″Lovibond(PFX 990 AOCS) | N/A | 70.0y 6.3R | AutoTintometerColour |
颜色,Gardner级,(1cm) | N/A | 12.0 | |
β-胡萝卜素(PFX990),ppm,(0.01cm) | N/A | 228.0 | |
注意:nottrue β-胡萝卜素 | |||
不皂化的成分,% | N/A | 2.11 | AOCS Ca 6b-53 |
不溶性杂质,% | N/A | 0.01 | AOCS Ca 3-46 |
AOM,小时 | N/A | 7.00 | AOCS Cd 12-57 |
Rancimat(80℃),小时 | N/A | 19.9 | |
自旋试验,按体积计的固体%,20℃/24小时 | N/A | 0.2 | |
脂肪酸组成(绝对值) | N/A | POS AS.SOP-104 | |
C12 | 3.9 | ||
C14 | 90.1 | ||
C14:1 | 0.8 | ||
C15 | 3.4 | ||
C16 | 193.9 | ||
C16:1 | 6.5 | ||
C18 | 4.8 | ||
C18:1 | 8.1 | ||
C18:2 | 3.6 | ||
C18:3n6 | 1.7 | ||
C18:4 | 2.6 | ||
C20 | 1.5 | ||
C20:4n6 | 4.9 | ||
C20:4n3 AA | 7.7 | ||
C20:5n3 EPA | 12.5 | ||
C22 | 0.8 |
C22:5n6+DPA | 129.7 | ||
C22:6n3 DHA | 385.5 | ||
C24 | 1.9 | ||
C24:1 | 1.6 | ||
其它 | 34.5 | ||
总计,mg/g | 900.0 | ||
DHA,FAME的% | 42.8 | ||
棕榈酸抗坏血酸酯,ppm | 222 | ||
生育酚,ppm | 1940 |
实验序号 | 己烷% | 丙酮/油比 | 冬化温度(C) | 冬化时间(H) | 过滤@(秒) | 油回收率(%) | 2周后的混浊度 |
1 | 1 | 1.5 | 5 | 3 | 67 | 87.8 | 澄清 |
2 | 2 | 1 | 0 | 2 | 165 | 86.4 | PPT |
3 | 2 | 1 | 0 | 4 | 195 | 87.7 | 澄清 |
4 | 2 | 1 | 10 | 2 | 178 | 88.1 | PPT |
5 | 2 | 1 | 10 | 4 | 154 | 89.8 | PPT |
6 | 2 | 2 | 0 | 2 | 85 | 84.1 | PPT |
7 | 2 | 2 | 0 | 4 | 75 | 86.2 | 澄清 |
8 | 2 | 2 | 10 | 2 | 67 | 88.9 | PPT |
9 | 2 | 2 | 10 | 4 | 82 | 86.7 | PPT |
10 | 3 | 0.5 | 5 | 3 | 264 | 84.3 | PPT |
11 | 3 | 1.5 | -5 | 3 | 102 | 83.4 | 澄清 |
12 | 3 | 1.5 | 5 | 1 | 87 | 85.5 | PPT |
13 | 3 | 1.5 | 5 | 3 | 109 | 85.4 | 澄清 |
14 | 3 | 1.5 | 5 | 3 | 123 | 86.3 | 澄清 |
15 | 3 | 1.5 | 5 | 3 | 82 | 87.5 | 澄清 |
16 | 3 | 1.5 | 5 | 3 | 110 | 87.9 | 澄清 |
17 | 3 | 1.5 | 5 | 5 | 117 | 86.6 | PPT |
18 | 3 | 1.5 | 15 | 3 | 255 | 94.8 | PPT |
19 | 3 | 2.5 | 5 | 3 | 73 | 87.2 | PPT |
20 | 4 | 1 | 0 | 2 | 262 | 87.5 | 澄清 |
21 | 4 | 1 | 0 | 4 | 115 | 91.2 | PPT |
22 | 4 | 1 | 10 | 2 | 245 | 83.7 | PPT |
23 | 4 | 1 | 10 | 4 | 375 | 86.7 | PPT |
24 | 4 | 2 | 0 | 2 | 52 | 88.4 | PPT |
25 | 4 | 2 | 0 | 4 | 80 | 89.3 | PPT |
26 | 4 | 2 | 10 | 2 | 92 | 86.8 | PPT |
27 | 4 | 2 | 10 | 4 | 83 | 88.7 | PPT |
28 | 5 | 1.5 | 5 | 3 | 86 | 87.1 | PPT |
对照组 | 150 | 90.9 | PPT* |
观察结果/分析 | 粗油 | 植物-己烷冬化的油 | 实验室-己烷冬化的油 | 丙酮-冬化的油(校验跟踪-1) | 丙酮-冬化的油(校验跟踪-2) |
油回收率(%) | 70% | 90.9 | |||
过滤@(秒) | - | 150 | |||
颜色(1″池)(1cm池) | 过暗70Y 11.2R | - | 70Y12.3R | 过暗70Y 12R | 过暗70Y 11.1R |
磷(ppm) | 474.3 | - | 474.0 | 271.6 | 144.3 |
游离脂肪酸 | 0.53 | - | 0.49 | 0.52 | 0.43 |
PV(meq/kg) | 0.00 | - | 1.82 | 3.32 | 4.27 |
茴香胺值 | 4.11 | - | 4.37 | 3.73 | 3.66 |
脂肪酸组成(mg/g) | |||||
C12:0 | 2.3 | - | 2.1 | 2.2 | 2.1 |
C14:0 | 67.2 | - | 57.8 | 58.5 | 58.9 |
C14:1 | 0.7 | - | 0.7 | 0.8 | 0.8 |
C15:0 | 3.3 | - | 3.1 | 3.1 | 3.2 |
C16:0 | 204.9 | - | 185.2 | 187.0 | 188.1 |
C16:1 | 3.3 | - | 3.5 | 3.6 | 3.5 |
C18:0 | 5.1 | - | 4.5 | 4.5 | 4.7 |
C18:1 | 3.9 | - | 4.0 | 4.0 | 4.0 |
C18:2 | 2.6 | - | 2.7 | 2.7 | 2.7 |
C18:3n6 | 2.3 | - | 2.5 | 2.5 | 2.6 |
C18:4 | 3.3 | - | 3.5 | 3.6 | 3.6 |
C20:0 | 1.2 | - | 1.0 | 1.0 | 1.0 |
C20:4n6 | 9.4 | - | 9.7 | 10.2 | 10.3 |
C20:4n3 | 8.0 | - | 8.3 | 8.5 | 8.6 |
C20:5n3 | 23.6 | - | 24.9 | 25.4 | 25.6 |
C22:0 | 0.6 | - | 0.6 | 0.5 | 0.6 |
C22:5n6 | 142.9 | - | 149.6 | 152.7 | 154.0 |
C22:6n3 | 351.1 | 369.0* | 369.0 | 378.6 | 382.2 |
C24:0 | 1.9 | - | 1.6 | 1.6 | 1.6 |
C24:1 | 4.0 | - | 4.1 | 4.3 | 4.2 |
其它 | 35.2 | - | 37.5 | 37.9 | 38.4 |
DHA的回收率 | 73% | 95.6% | 93.8% | 92.8% |
观察结果/分析 | 丙酮-冬化的蜡(校验跟踪-1) | 丙酮-冬化的蜡(校验跟踪-2) |
蜡回收率(%) | 13.1 | 14.7 |
脂肪酸组成(mg/g) | ||
C12:0 | 2.8 | 2.6 |
C14:0 | 112.4 | 103.2 |
C14:1 | 0.4 | 0.4 |
C15:0 | 3.8 | 0.6 |
C16:0 | 303.4 | 282.6 |
C16:1 | 2.1 | 2.1 |
C18:0 | 8.6 | 8.7 |
C18:1 | 3.3 | 3.6 |
C18:2 | 1.3 | 1.7 |
C18:3n6 | 1.1 | 1.0 |
C18:4 | 1.5 | 1.4 |
C20:0 | 2.2 | 2.0 |
C20:4n6 | 4.9 | 4.6 |
C20:4n3 | 4.1 | 4.0 |
C20:5n3 | 11.9 | 11.9 |
C22:0 | 1.3 | 1.2 |
C22:5n6 | 76.8 | 75.7 |
C22:6n3 | 175.2 | 170.5 |
C24:0 | 3.8 | 3.5 |
C24:1 | 2.1 | 2.0 |
其它 | 16.6 | 18.1 |
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Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE60143287D1 (de) | 2000-01-19 | 2010-12-02 | Martek Biosciences Corp | Lösungsmittelfreies extraktionsverfahren |
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MX2009001346A (es) | 2006-08-01 | 2009-04-17 | Ocean Nutrition Canada Ltd | Microbios productores de aceite y metodos de modificacion de los mismos. |
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US8901331B2 (en) * | 2008-03-17 | 2014-12-02 | Stepan Specialty Products, Llc | Process for refining a triglyceride oil |
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US9896642B2 (en) | 2008-10-14 | 2018-02-20 | Corbion Biotech, Inc. | Methods of microbial oil extraction and separation |
US9480271B2 (en) | 2009-09-15 | 2016-11-01 | Monsanto Technology Llc | Soybean seed and oil compositions and methods of making same |
US8552098B2 (en) * | 2009-09-30 | 2013-10-08 | Dow Global Technologies Llc | Purified acetylated derivatives of castor oil and compositions including same |
US20130101723A1 (en) * | 2009-11-03 | 2013-04-25 | Daniel Verkoeijen | Vegetable oil comprising a polyunsaturated fatty acid having at least 20 carbon atoms |
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US8187861B1 (en) | 2010-01-26 | 2012-05-29 | Allen John Schuh | Phosphate removal-recovery and biofuel feedstock system |
US9693577B2 (en) | 2010-01-29 | 2017-07-04 | Abbott Laboratories | Method of preparing a nutritional powder comprising spray dried HMB |
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MX2012008785A (es) | 2010-01-29 | 2012-08-17 | Abbott Lab | Emulsiones nutricionales que comprenden beta-hidroxi-beta-metilbut irato (hmb) de calcio. |
US8211309B2 (en) | 2010-04-06 | 2012-07-03 | Heliae Development, Llc | Extraction of proteins by a two solvent method |
US8313648B2 (en) | 2010-04-06 | 2012-11-20 | Heliae Development, Llc | Methods of and systems for producing biofuels from algal oil |
KR20130041802A (ko) | 2010-04-06 | 2013-04-25 | 헬리아에 디벨롭먼트, 엘엘씨 | 감소하는 극성의 용매를 사용하는, 유지성 물질로부터 오일 및 단백질성 물질의 연속 용매 추출법 |
US8273248B1 (en) | 2010-04-06 | 2012-09-25 | Heliae Development, Llc | Extraction of neutral lipids by a two solvent method |
US20130217904A1 (en) * | 2010-04-06 | 2013-08-22 | Heliae Development, Llc | Method of extracting polar lipids and neutral lipids with two solvents |
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US8115022B2 (en) | 2010-04-06 | 2012-02-14 | Heliae Development, Llc | Methods of producing biofuels, chlorophylls and carotenoids |
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WO2011127127A2 (en) | 2010-04-06 | 2011-10-13 | Arizona Board Of Regents For And On Behalf Of Arizona State University | Extraction with fractionation of oil and co-products from oleaginous material |
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TWI526161B (zh) | 2010-06-10 | 2016-03-21 | 亞培公司 | 包含鈣hmb及可溶性蛋白質之實質上透明營養液 |
MY163938A (en) | 2010-06-14 | 2017-11-15 | Io-Mega Holding Corp | Method for the production of algae derived oils |
WO2012109545A2 (en) | 2011-02-11 | 2012-08-16 | E. I. Du Pont De Nemours And Company | Method for obtaining a lipid-containing composition from microbial biomass |
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US8365462B2 (en) | 2011-05-31 | 2013-02-05 | Heliae Development, Llc | V-Trough photobioreactor systems |
USD679965S1 (en) | 2011-06-10 | 2013-04-16 | Heliae Development, Llc | Aquaculture vessel |
USD682637S1 (en) | 2011-06-10 | 2013-05-21 | Heliae Development, Llc | Aquaculture vessel |
USD661164S1 (en) | 2011-06-10 | 2012-06-05 | Heliae Development, Llc | Aquaculture vessel |
US10383839B2 (en) * | 2011-06-30 | 2019-08-20 | Johnson & Johnson Vision Care, Inc. | Esters for treatment of ocular inflammatory conditions |
CN103930104A (zh) | 2011-07-21 | 2014-07-16 | 帝斯曼知识产权资产管理有限公司 | 脂肪酸组合物 |
WO2013075116A2 (en) | 2011-11-17 | 2013-05-23 | Heliae Development, Llc | Omega 7 rich compositions and methods of isolating omega 7 fatty acids |
WO2013172087A1 (ja) * | 2012-05-17 | 2013-11-21 | 株式会社竹田測量設計 | 一酸化窒素産生阻害活性を備える組成物 |
JP2016509305A (ja) | 2013-01-31 | 2016-03-24 | ヒューレット−パッカード デベロップメント カンパニー エル.ピー.Hewlett‐Packard Development Company, L.P. | ファームウェアインタフェース変数リポジトリに書き込まれるデータを示すためのコミットリストの更新 |
US9873880B2 (en) | 2013-03-13 | 2018-01-23 | Dsm Nutritional Products Ag | Engineering microorganisms |
MX2016008223A (es) | 2013-12-20 | 2016-11-28 | Dsm Ip Assets Bv | Procedimiento para la obtención de aceite microbiano a partir de células microbianas. |
US10342772B2 (en) | 2013-12-20 | 2019-07-09 | Dsm Ip Assets B.V. | Processes for obtaining microbial oil from microbial cells |
EP3082792A4 (en) | 2013-12-20 | 2017-08-16 | DSM IP Assets B.V. | Processes for obtaining microbial oil from microbial cells |
AR098893A1 (es) | 2013-12-20 | 2016-06-22 | Dsm Ip Assets Bv | Proceso para obtener aceite microbiano a partir de células microbianas |
RU2689556C2 (ru) * | 2014-05-30 | 2019-05-28 | Драй Лилиен Пфг Гмбх Унд Ко. Кг | Способ очистки подвергнутых рафинированию липидных фаз |
AR104042A1 (es) | 2015-03-26 | 2017-06-21 | Mara Renewables Corp | Producción de alta densidad de biomasa y aceite utilizando glicerol en bruto |
KR20180027584A (ko) | 2015-07-13 | 2018-03-14 | 마라 리뉴어블즈 코퍼레이션 | 크실로오스의 미세조류 대사를 강화시키는 방법 |
US10851395B2 (en) | 2016-06-10 | 2020-12-01 | MARA Renewables Corporation | Method of making lipids with improved cold flow properties |
BR112019000435A2 (pt) | 2016-07-13 | 2019-04-30 | Evonik Degussa Gmbh | método para separar lipídios de uma biomassa contendo lipídios lisados |
US11352651B2 (en) | 2016-12-27 | 2022-06-07 | Evonik Operations Gmbh | Method of isolating lipids from a lipids containing biomass |
EP3585369A4 (en) * | 2017-02-22 | 2020-08-26 | Cargill, Incorporated | CLEANING OF OILS CONTAINING DHA |
EP3470502A1 (en) * | 2017-10-13 | 2019-04-17 | Evonik Degussa GmbH | Method of separating lipids from a lysed lipids containing biomass |
WO2019048327A1 (en) * | 2017-09-05 | 2019-03-14 | Evonik Degussa Gmbh | METHOD FOR SEPARATING LIPIDS IN A BIOMASS CONTAINING LYDE LIPIDS |
EP3527664A1 (en) | 2018-02-15 | 2019-08-21 | Evonik Degussa GmbH | Method of isolating lipids from a lipids containing biomass |
US11414621B2 (en) | 2018-05-15 | 2022-08-16 | Evonik Operations Gmbh | Method of isolating lipids from a lipids containing biomass with aid of hydrophobic silica |
WO2019219396A1 (en) | 2018-05-15 | 2019-11-21 | Evonik Operations Gmbh | Method of isolating lipids from a lysed lipids containing biomass by emulsion inversion |
EP3586640A1 (en) | 2018-06-21 | 2020-01-01 | Nuseed Pty Ltd | Dha enriched polyunsaturated fatty acid compositions |
FR3085825B1 (fr) | 2018-09-14 | 2021-07-16 | Fermentalg | Huile de microorganismes riche en acide docosahexaenoique |
FR3085962B1 (fr) | 2018-09-14 | 2021-06-18 | Fermentalg | Procede d'extracton d'une huile riche en pufa |
CN109731102B (zh) * | 2019-01-31 | 2022-05-17 | 广州白云山汉方现代药业有限公司 | 一种降低橄榄油甲氧基苯胺值的方法 |
Family Cites Families (33)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3541123A (en) * | 1968-10-21 | 1970-11-17 | Kao Corp | Process for the fractionation of oils and fats |
US4205006A (en) * | 1973-03-05 | 1980-05-27 | The United States Of America As Represented By The Secretary Of Agriculture | Physiochemically designed fat compositions from tallow and process for making |
US3944585A (en) * | 1973-03-05 | 1976-03-16 | The United States Of America As Represented By The Secretary Of Agriculture | Multi-step crystallization and blending process for making physiochemically designed fat compositions from tallow |
JPS5959644A (ja) * | 1982-09-29 | 1984-04-05 | Kureha Chem Ind Co Ltd | 魚油中のエイコサペンタエン酸を濃縮する方法 |
JPS5967241A (ja) * | 1982-10-08 | 1984-04-16 | Kureha Chem Ind Co Ltd | 魚油中のエイコサペンタエン酸の濃縮方法 |
JPS60133094A (ja) * | 1983-12-21 | 1985-07-16 | 日清製油株式会社 | 高純度エイコサペンタエン酸の製造法 |
JPS61192292A (ja) * | 1985-02-21 | 1986-08-26 | Agency Of Ind Science & Technol | γ−リノレン酸含有グリセリドの濃縮方法 |
DE3587044T2 (de) * | 1985-01-22 | 1993-06-03 | Agency Ind Science Techn | Verfahren zur herstellung von lipiden aus fungusmassen. |
US4792418A (en) * | 1985-08-14 | 1988-12-20 | Century Laboratories, Inc. | Method of extraction and purification of polyunsaturated fatty acids from natural sources |
US5336792A (en) | 1990-03-12 | 1994-08-09 | Einar Sola | Process for enrichment of fat with regard to polyunsaturated fatty acids and phospholipids, and application of such enriched fat |
US5658767A (en) | 1991-01-24 | 1997-08-19 | Martek Corporation | Arachidonic acid and methods for the production and use thereof |
JP3354582B2 (ja) * | 1991-09-30 | 2002-12-09 | サントリー株式会社 | オメガ9系高度不飽和脂肪酸およびこれを含有する脂質の製造方法 |
DE4219360C2 (de) | 1992-06-12 | 1994-07-28 | Milupa Ag | Verfahren zur Gewinnung von Lipiden mit einem hohen Anteil von langkettig-hochungesättigten Fettsäuren |
ATE199481T1 (de) * | 1995-11-24 | 2001-03-15 | Loders Croklaan Bv | Zusammensetzung auf basis von fischöl |
CN101928213B (zh) * | 1996-03-28 | 2012-12-05 | Dsmip资产有限公司 | 粒状微生物生物质的制备及其有价值的化合物的分离方法 |
US6255505B1 (en) * | 1996-03-28 | 2001-07-03 | Gist-Brocades, B.V. | Microbial polyunsaturated fatty acid containing oil from pasteurised biomass |
JP2000510513A (ja) * | 1996-05-15 | 2000-08-15 | ヒスト―ブロカデス・ベスローテン・フェンノートシャップ | 低ステロール且つ高トリグリセライドの微生物性油を得るための極性溶媒によるステロール抽出 |
DE69737063T2 (de) | 1996-07-23 | 2007-07-19 | Nagase Chemtex Corp. | Verfahren zur herstellung von decosahexaensäure und decosapentsäure |
US6140486A (en) * | 1997-06-04 | 2000-10-31 | Calgene Llc | Production of polyunsaturated fatty acids by expression of polyketide-like synthesis genes in plants |
DE60143287D1 (de) * | 2000-01-19 | 2010-12-02 | Martek Biosciences Corp | Lösungsmittelfreies extraktionsverfahren |
JP3425622B2 (ja) * | 2000-03-30 | 2003-07-14 | 独立行政法人産業技術総合研究所 | ラビリンチュラ属菌を用いた高度不飽和脂肪酸含有培養物および高度不飽和脂肪酸含有油脂の製造方法 |
US6344574B1 (en) | 2000-07-20 | 2002-02-05 | The United States Of America As Represented By The Secretary Of Agriculture | Solvent fractionation of chicken fat for making lipid compositions enriched in unsaturated fatty acid-containing triacylglycerols |
EP1178103A1 (en) * | 2000-08-02 | 2002-02-06 | Dsm N.V. | Purifying crude pufa oils |
ATE510908T1 (de) * | 2000-09-28 | 2011-06-15 | Bioriginal Food & Science Corp | Fad5-2, mitglied der desaturase-familie und verwendungen davon |
CN1109093C (zh) * | 2000-09-28 | 2003-05-21 | 中国科学院武汉病毒研究所 | 一种微生物油脂的制备方法 |
EP1215274A1 (en) * | 2000-12-15 | 2002-06-19 | Dsm N.V. | Enrichment of microbial oils |
US6492537B2 (en) | 2001-02-20 | 2002-12-10 | The United States Of America As Represented By The Secretary Of Agriculture | Solvent fractionation of menhaden oil and partially hydrogenated menhaden oil for making lipid compositions enriched in unsaturated fatty acid-containing triacylglycerols |
DE10151155A1 (de) | 2001-10-19 | 2003-05-08 | Nutrinova Gmbh | Native PUFA-Triglyceridmischungen mit einem hohen Gehalt an mehrfach ungesättigten Fettsäuren sowie Verfahren zu deren Herstellung und deren Verwendung |
ATE522264T1 (de) * | 2001-12-12 | 2011-09-15 | Martek Biosciences Corp | Extrahieren und entstearinieren von lipiden aus biomasse |
US20040209953A1 (en) * | 2002-12-06 | 2004-10-21 | Wai Lee Theresa Siu-Ling | Glyceride compositions and methods of making and using same |
US7561412B2 (en) * | 2006-09-29 | 2009-07-14 | Rockwell Automation Technologies, Inc. | System and method for automatically securing a motor control center |
US7528612B2 (en) * | 2006-09-29 | 2009-05-05 | Rockwell Automation Technologies, Inc. | System and method for monitoring a motor control center |
US8228067B2 (en) * | 2006-11-02 | 2012-07-24 | Production Resource Group, Llc | Load bank |
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2002
- 2002-12-12 AT AT02791414T patent/ATE522264T1/de not_active IP Right Cessation
- 2002-12-12 AU AU2002366642A patent/AU2002366642B2/en not_active Ceased
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- 2002-12-12 JP JP2003550880A patent/JP4647212B2/ja not_active Expired - Fee Related
- 2002-12-12 WO PCT/US2002/039930 patent/WO2003049832A1/en active Application Filing
- 2002-12-12 EP EP10166318A patent/EP2239028A1/en not_active Withdrawn
- 2002-12-12 EP EP10176356A patent/EP2261312A1/en not_active Withdrawn
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- 2002-12-12 CA CA2469647A patent/CA2469647C/en not_active Expired - Fee Related
- 2002-12-12 EP EP02791414A patent/EP1453583B1/en not_active Revoked
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2004
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2008
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2009
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2010
- 2010-03-16 JP JP2010058682A patent/JP2010196060A/ja active Pending
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2011
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- 2011-11-10 AU AU2011250697A patent/AU2011250697A1/en not_active Abandoned
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2013
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Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
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CN101098629B (zh) * | 2004-11-04 | 2010-10-13 | 孟山都技术公司 | 高pufa油组合物 |
CN103328620A (zh) * | 2010-11-08 | 2013-09-25 | 耐思特石油公司 | 一种从生物质中提取脂质的方法 |
CN103328620B (zh) * | 2010-11-08 | 2014-08-27 | 耐思特石油公司 | 一种从生物质中提取脂质的方法 |
CN103156003A (zh) * | 2011-12-16 | 2013-06-19 | 丰益(上海)生物技术研发中心有限公司 | 一种调和油及其制备方法 |
CN103156003B (zh) * | 2011-12-16 | 2015-04-29 | 丰益(上海)生物技术研发中心有限公司 | 一种调和油及其制备方法 |
CN107257630A (zh) * | 2014-11-14 | 2017-10-17 | 巴斯夫植物科学有限公司 | 包含pufa的植物脂质的修饰 |
CN106343045A (zh) * | 2016-08-11 | 2017-01-25 | 青岛俏宝食品有限公司 | 一种富含长碳链多不饱和脂肪酸类脂质的营养强化型功能低温食用调和油脂 |
CN112218648A (zh) * | 2018-04-20 | 2021-01-12 | 纳米藻类溶液公司 | 提供二十碳五烯酸油组合物的新方法 |
Also Published As
Publication number | Publication date |
---|---|
JP2013100530A (ja) | 2013-05-23 |
US8012354B2 (en) | 2011-09-06 |
AU2009200718B2 (en) | 2011-08-11 |
US20090099379A1 (en) | 2009-04-16 |
WO2003049832A1 (en) | 2003-06-19 |
CN1267174C (zh) | 2006-08-02 |
US20100261919A1 (en) | 2010-10-14 |
US20050115897A1 (en) | 2005-06-02 |
JP4647212B2 (ja) | 2011-03-09 |
EP2239028A1 (en) | 2010-10-13 |
US7419596B2 (en) | 2008-09-02 |
AU2011250697A1 (en) | 2011-12-01 |
AU2009200718A1 (en) | 2009-03-19 |
JP2010196060A (ja) | 2010-09-09 |
NO20042929L (no) | 2004-07-08 |
AU2002366642B2 (en) | 2008-12-11 |
ATE522264T1 (de) | 2011-09-15 |
EP1453583A2 (en) | 2004-09-08 |
US7695626B2 (en) | 2010-04-13 |
EP1453583B1 (en) | 2011-08-31 |
JP2005513051A (ja) | 2005-05-12 |
CA2718374C (en) | 2013-05-07 |
US20120059180A1 (en) | 2012-03-08 |
EP2261312A1 (en) | 2010-12-15 |
US8480904B2 (en) | 2013-07-09 |
CA2469647A1 (en) | 2003-06-19 |
CA2718374A1 (en) | 2003-06-19 |
EP1453583A4 (en) | 2005-08-17 |
AU2002366642A1 (en) | 2003-06-23 |
WO2003049832A8 (en) | 2003-10-23 |
CA2469647C (en) | 2011-02-15 |
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