CN1556850B - 转化破囊壶菌目微生物的产物和方法 - Google Patents

转化破囊壶菌目微生物的产物和方法 Download PDF

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Publication number
CN1556850B
CN1556850B CN028120590A CN02812059A CN1556850B CN 1556850 B CN1556850 B CN 1556850B CN 028120590 A CN028120590 A CN 028120590A CN 02812059 A CN02812059 A CN 02812059A CN 1556850 B CN1556850 B CN 1556850B
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nucleic acid
seq
sequence
acid sequence
acetolactate synthase
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CN1556850A (zh
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保罗·G·罗斯勒
T·戴夫·马修斯
汤姆·M·拉姆西尔
詹姆斯·G·梅茨
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y202/00—Transferases transferring aldehyde or ketonic groups (2.2)
    • C12Y202/01—Transketolases and transaldolases (2.2.1)
    • C12Y202/01006—Acetolactate synthase (2.2.1.6)

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Mushroom Cultivation (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
CN028120590A 2001-04-16 2002-04-16 转化破囊壶菌目微生物的产物和方法 Expired - Fee Related CN1556850B (zh)

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US28411601P 2001-04-16 2001-04-16
US60/284,116 2001-04-16
PCT/US2002/012040 WO2002083869A2 (en) 2001-04-16 2002-04-16 Product and process for transformation of thraustochytriales microorganisms

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CNB2006100842683A Division CN100513564C (zh) 2001-04-16 2002-04-16 转化破囊壶菌目微生物的产物和方法
CN200910141898A Division CN101760469A (zh) 2001-04-16 2002-04-16 转化破囊壶菌目微生物的产物和方法

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CN1556850B true CN1556850B (zh) 2012-09-26

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CN200910141898A Pending CN101760469A (zh) 2001-04-16 2002-04-16 转化破囊壶菌目微生物的产物和方法
CNB2006100842683A Expired - Fee Related CN100513564C (zh) 2001-04-16 2002-04-16 转化破囊壶菌目微生物的产物和方法

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CNB2006100842683A Expired - Fee Related CN100513564C (zh) 2001-04-16 2002-04-16 转化破囊壶菌目微生物的产物和方法

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US (5) US7001772B2 (enExample)
EP (3) EP2325319B1 (enExample)
JP (4) JP4536324B2 (enExample)
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AT (1) ATE533844T1 (enExample)
AU (1) AU2002250592C1 (enExample)
CA (1) CA2443908C (enExample)
ES (3) ES2377557T3 (enExample)
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Families Citing this family (78)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6566583B1 (en) * 1997-06-04 2003-05-20 Daniel Facciotti Schizochytrium PKS genes
US8003772B2 (en) 1999-01-14 2011-08-23 Martek Biosciences Corporation Chimeric PUFA polyketide synthase systems and uses thereof
US7247461B2 (en) * 1999-01-14 2007-07-24 Martek Biosciences Corporation Nucleic acid molecule encoding ORFA of a PUFA polyketide synthase system and uses thereof
US20070244192A1 (en) * 1999-01-14 2007-10-18 Martek Biosciences Corporation Plant seed oils containing polyunsaturated fatty acids
US7211418B2 (en) * 1999-01-14 2007-05-01 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
US7217856B2 (en) * 1999-01-14 2007-05-15 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
TWI324181B (en) * 2001-04-16 2010-05-01 Martek Biosciences Corp Product and process for transformation of thraustochytriales microorganisms
US7211656B2 (en) † 2002-01-30 2007-05-01 Abbott Laboratories Desaturase genes, enzymes encoded thereby, and uses thereof
EP1572994B1 (en) * 2002-12-20 2007-02-21 Chromagenics B.V. Means and methods for producing a protein through chromatin openers that are capable of rendering chromatin more accessible to transcription factors
US7208590B2 (en) * 2003-07-15 2007-04-24 Abbott Laboratories Genes involved in polyketide synthase pathways and uses thereof
ES2529572T3 (es) 2004-04-22 2015-02-23 Commonwealth Scientific And Industrial Research Organisation Síntesis de ácidos grasos poliinsaturados de cadena larga por células recombinantes
PL1756280T3 (pl) 2004-04-22 2015-04-30 Commw Scient Ind Res Org Synteza długołańcuchowych wielonienasyconych kwasów tłuszczowych w komórkach rekombinowanych
CN101090968B (zh) * 2004-11-08 2011-10-26 科罗迈吉尼科斯公司 高水平表达蛋白质的宿主细胞的选择
US20100136616A1 (en) * 2004-11-08 2010-06-03 Chromagenics B.V. Selection of Host Cells Expressing Protein at High Levels
US8999667B2 (en) * 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
US20060195935A1 (en) 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
KR101271884B1 (ko) * 2004-11-08 2013-06-05 크로마제닉스 비.브이. 고수준으로 단백질을 발현하는 숙주세포의 선택
JP4796787B2 (ja) * 2005-04-28 2011-10-19 富士フイルム株式会社 ラビリンチュラ類への遺伝子導入法
JP4796786B2 (ja) * 2005-04-28 2011-10-19 富士フイルム株式会社 ラビリンチュラ類を形質転換可能なベクター
MX338235B (es) 2005-06-07 2016-04-08 Dsm Nutritional Products Ag Microorganismos eucarioticos para producir lipidos y antioxidantes.
WO2007061742A1 (en) 2005-11-23 2007-05-31 E. I. Du Pont De Nemours And Company Delta-9 elongases and their use in making polyunsaturated fatty acids
EP1818411A1 (en) * 2006-02-13 2007-08-15 Lonza AG Process for the preparation of optically active chiral amines
MX340759B (es) * 2006-03-15 2015-03-11 Dsm Ip Assets B V * Aceites de semillas vegetales que contienen ácidos grasos poli-insaturados.
EP2007215A4 (en) * 2006-04-11 2011-04-13 Martek Biosciences Corp FOODSTUFFS WITH LONG-CHAIN MULTIPLE-UNSATURATED FATTY ACIDS AND METHOD FOR THE PRODUCTION THEREOF
CA2655044C (en) 2006-06-22 2014-05-27 Martek Biosciences Corporation Encapsulated labile compound compositions and methods of making the same
EP2082053B1 (en) * 2006-08-01 2016-04-13 DSM Nutritional Products AG Process for producing microbial oil comprising polyunsaturated fatty acids
TW200824582A (en) 2006-08-29 2008-06-16 Martek Biosciences Corp Use of DPA(n-6) oils in infant formula
BRPI0716075A2 (pt) 2006-08-29 2013-08-06 Commw Scient Ind Res Org sÍntese de Ácidos graxos
KR100860111B1 (ko) * 2006-10-02 2008-09-25 인제대학교 산학협력단 신규 미생물 트라우스토카이트리움 에스피.케이제이에스-1, 바실러스 폴리퍼멘티쿠스 케이제이에스-2 및 이들과 바실러스 리케니포르미스를 포함하고 있는 사료첨가제 및 사료
CA2663768A1 (en) 2006-10-23 2008-05-29 E. I. Du Pont De Nemours And Company Delta-8 desaturases and their use in making polyunsaturated fatty acids
US8188338B2 (en) 2007-04-10 2012-05-29 E. I. Du Pont De Nemours And Company Delta-8 desaturases and their use in making polyunsaturated fatty acids
US8119860B2 (en) 2007-04-16 2012-02-21 E. I. Du Pont De Nemours And Company Delta-9 elongases and their use in making polyunsaturated fatty acids
US7943365B2 (en) 2007-05-03 2011-05-17 E.I. Du Pont De Nemours And Company Δ-5 desaturases and their use in making polyunsaturated fatty acids
AU2008269989B2 (en) * 2007-06-29 2014-02-27 Dsm Ip Assets B.V. Production and purification of esters of polyunsaturated fatty acids
NZ598199A (en) 2007-09-12 2013-12-20 Martek Biosciences Corp Biological oils and production and uses thereof
US20110076379A1 (en) * 2008-06-13 2011-03-31 Means Michael M High omega saturated fat free meat products
CA3015426C (en) 2008-11-18 2020-01-14 Commonwealth Scientific And Industrial Research Organisation Recombinant cells comprising exogenous .delta.5 and .delta.6 desaturases, .delta.5 and .delta.6 elongases, and .delta.4 desaturases
CA2744716C (en) 2008-12-18 2017-05-23 E.I. Du Pont De Nemours And Company Reducing byproduction of malonates in a fermentation process
JP5415896B2 (ja) 2009-01-29 2014-02-12 Ｊｆｅスチール株式会社 インダイレクトスポット溶接方法
ES2752196T3 (es) * 2009-03-16 2020-04-03 Dsm Ip Assets Bv Producción de proteínas en microorganismos del filo Labyrinthulomycota
US8207363B2 (en) * 2009-03-19 2012-06-26 Martek Biosciences Corporation Thraustochytrids, fatty acid compositions, and methods of making and uses thereof
ES2717102T3 (es) * 2009-03-19 2019-06-19 Dsm Ip Assets Bv Moléculas de ácido nucleico de ácido graso poliinsaturado sintasas, y polipéptidos, composiciones y métodos de preparación y usos de los mismos
BRPI1009690A2 (pt) 2009-06-16 2015-08-25 Du Pont "célula hospedeira microbiana oleaginosa, óleo, método de elaboração de um óleo, método de aumento da eficiência de conversão de alongamento de c18 para c20 em uma célula hospedeira recombinante microbiana oleaginosa e método de aumento da eficiência de conversão de dessaturação (delta) 4 em uma célula hospedeira recombinante microbiana oleaginosa produtora de ácidos graxos póli-insaturados de cadeia longa"
EP2481799A4 (en) * 2009-09-24 2013-08-07 Univ Kyushu Nat Univ Corp METHOD FOR THE TRANSFORMATION OF STRAMENOPILES
CN103068965A (zh) 2009-12-28 2013-04-24 Dsmip资产公司 在蔗糖上生长的重组破囊壶菌和其组合物、制备方法及用途
WO2011090708A2 (en) 2009-12-28 2011-07-28 Merial Limited Recombinant ndv antigen and uses thereof
CN107746869A (zh) * 2009-12-28 2018-03-02 赛诺菲疫苗技术公司 微藻中异源多肽的生产，微藻胞外体、组合物及其制备方法和用途
US20110195448A1 (en) * 2009-12-28 2011-08-11 James Casey Lippmeier Recombinant Thraustochytrids that Grow on Xylose, and Compositions, Methods of Making, and Uses Thereof
IN2012DN06278A (enExample) * 2009-12-28 2015-09-25 Dsm Ip Assets Bv
CA2993690C (en) * 2010-01-19 2022-03-22 Dsm Ip Assets B.V. Eicosapentaenoic acid-producing microorganisms, fatty acid compositions, and methods of making and uses thereof
US9345759B2 (en) 2010-03-12 2016-05-24 Merial, Inc. Bluetongue virus recombinant vaccines and uses thereof
AU2011293189B2 (en) 2010-08-26 2017-02-16 E. I. Du Pont De Nemours And Company Mutant HPGG motif and HDASH motif delta-5 desaturases and their use in making polyunsaturated fatty acids
WO2012027676A1 (en) 2010-08-26 2012-03-01 E. I. Du Pont De Nemours And Company Mutant delta-9 elongases and their use in making polyunsaturated fatty acids
PT2683824T (pt) * 2011-03-07 2018-06-07 Dsm Nutritional Products Ag Modificação de microorganismos thraustochytrid
EA035287B1 (ru) 2011-07-21 2020-05-25 ДСМ АйПи АССЕТС Б.В. Микроорганизмы, продуцирующие эйкозапентаеновую кислоту, композиции, содержащие жирные кислоты, и способы их получения и применения
HUE033766T2 (en) 2012-06-15 2018-01-29 Commw Scient Ind Res Org Production of long chain polyunsaturated fatty acids in plant cells
WO2013192007A1 (en) 2012-06-19 2013-12-27 E. I. Du Pont De Nemours And Company MUTANT ACYL-CoA:LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASES
EP2970926B1 (en) 2013-03-13 2018-01-31 DSM Nutritional Products AG Engineering microorganisms
EA037817B1 (ru) 2013-12-18 2021-05-25 Коммонвелт Сайнтифик Энд Индастриэл Рисерч Организэйшн Экстрагированный растительный липид, содержащий длинноцепочечные полиненасыщенные жирные кислоты
CA2953008C (en) 2014-06-27 2024-03-19 Nuseed Pty Ltd Lipid comprising docosapentaenoic acid
CN105567715B (zh) * 2014-10-17 2021-06-29 丰益(上海)生物技术研发中心有限公司 裂殖壶菌α-微管蛋白相关序列及其应用
US9932599B2 (en) 2015-03-02 2018-04-03 Synthetic Genomics, Inc. Regulatory elements from labyrinthulomycetes microorganisms
AR104042A1 (es) 2015-03-26 2017-06-21 Mara Renewables Corp Producción de alta densidad de biomasa y aceite utilizando glicerol en bruto
PL3302032T3 (pl) 2015-06-06 2022-06-20 Dsm Ip Assets B.V. Wytwarzanie wielonienasyconych kwasów tłuszczowych (PUFA) z zastosowaniem nowej, modularnej syntazy kwasu dokozaheksaenowego (DHA)
US10542961B2 (en) 2015-06-15 2020-01-28 The Research Foundation For The State University Of New York System and method for infrasonic cardiac monitoring
JP6977231B2 (ja) 2015-07-13 2021-12-08 マラ リニューアブルズ コーポレーション Ｃ５有機炭素の微生物による代謝の増強
IL241462A0 (en) 2015-09-10 2015-11-30 Yeda Res & Dev Heterologous engineering of betalain pigments in plants
EP3430035A4 (en) 2016-03-16 2020-02-19 Conagen Inc. PRODUCTION OF PROTEINS IN LABYRINE THULOMYCETES
JP6966040B2 (ja) 2016-05-12 2021-11-10 ディーエスエム アイピー アセッツ ビー.ブイ.Dsm Ip Assets B.V. 微細藻類中でのオメガ−３多価不飽和脂肪酸産生を増加させる方法
US10851395B2 (en) 2016-06-10 2020-12-01 MARA Renewables Corporation Method of making lipids with improved cold flow properties
JP2019520069A (ja) 2016-07-13 2019-07-18 ディーエスエム アイピー アセッツ ビー．ブイ．Ｄｓｍ Ｉｐ Ａｓｓｅｔｓ Ｂ．Ｖ． 藻類宿主細胞用のｃｒｉｓｐｒ−ｃａｓシステム
IL247752A0 (en) 2016-09-11 2016-11-30 Yeda Res & Dev Compositions and methods for modulating gene expression for site-directed mutagenesis
US10633454B2 (en) 2016-11-01 2020-04-28 Conagen Inc. Expression of modified glycoproteins and glycopeptides
WO2019078359A1 (ja) 2017-10-19 2019-04-25 味の素株式会社 ステロールの製造法
WO2019173226A1 (en) * 2018-03-05 2019-09-12 Synthetic Genomics, Inc. Organisms and methods for producing glycomolecules with low sulfation
EP3788164A4 (en) * 2018-05-01 2022-01-26 Conagen Inc. Recombinant organisms and methods for producing glycomolecules with high glycan occupancy
EP3835410A4 (en) * 2018-08-10 2022-05-18 Kyowa Hakko Bio Co., Ltd. MICROORGANISM PRODUCING EICOSAPENTAENOIC ACID AND METHOD FOR PRODUCTION OF EICOSAPENTAENOIC ACID
CN109777815B (zh) * 2019-03-28 2021-10-29 昆明理工大学 HMG-CoA合成酶基因RKHMGCS及其应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4381897A (en) * 1980-10-06 1983-05-03 Krupp Polysius Ag Installation for transporting fine-grained material

Family Cites Families (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
LU35839A1 (enExample)
BE545283A (enExample)
US3223456A (en) * 1963-11-14 1965-12-14 Siemens Ag Conveying apparatus for fine-granular material
US4626505A (en) 1984-02-24 1986-12-02 E. I. Du Pont De Nemours And Company Selectable markers for yeast transformation
US5605011A (en) * 1986-08-26 1997-02-25 E. I. Du Pont De Nemours And Company Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase
IL83348A (en) * 1986-08-26 1995-12-08 Du Pont Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase
US5340742A (en) 1988-09-07 1994-08-23 Omegatech Inc. Process for growing thraustochytrium and schizochytrium using non-chloride salts to produce a microfloral biomass having omega-3-highly unsaturated fatty acids
US5130242A (en) * 1988-09-07 1992-07-14 Phycotech, Inc. Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids
US5426040A (en) * 1989-08-17 1995-06-20 Northeastern University Methods for producing improved strains of seaweed by fusion of spore-protoplasts, and resultant seaweeds and phycocolloids
IL100908A0 (en) 1991-02-22 1992-11-15 Salk Inst Biotech Ind Invertase genes and their use
US5804408A (en) 1991-03-13 1998-09-08 Yoshihide Hagiwara Expression of human SOD in blue green algae
US5661017A (en) 1993-09-14 1997-08-26 Dunahay; Terri Goodman Method to transform algae, materials therefor, and products produced thereby
IL113375A (en) * 1995-04-13 1997-09-30 Fortress U & T Ltd Internationally regulated system for one to one cryptographic communications with national sovereignty without key escrow
JP4469422B2 (ja) * 1995-04-20 2010-05-26 アメリカン シアナミド コンパニー 構造に基づきデザインされた除草剤耐性生産物
US5853973A (en) * 1995-04-20 1998-12-29 American Cyanamid Company Structure based designed herbicide resistant products
DE19529475A1 (de) 1995-08-11 1997-02-13 Marcus Weis Siebkonstruktion zum Abtrennen des Faseranteils aus der Shredderleichtfraktion
US5824309A (en) 1995-12-05 1998-10-20 University Of Massachusetts Recombinant gas vesicles and uses thereof
WO1997039106A1 (en) 1996-04-12 1997-10-23 Martek Biosciences Corporation Methods and tools for transformation of eukaryotic algae
US6194167B1 (en) * 1997-02-18 2001-02-27 Washington State University Research Foundation ω-3 fatty acid desaturase
US5968809A (en) * 1997-04-11 1999-10-19 Abbot Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids
ES2125195B1 (es) 1997-04-18 1999-10-01 Antibioticos Sa Procedimiento de inactivacion de genes que codifican para enzimas del catabolismo del fenilacetato, plasmidos que intervienen y cepas transformadas con los mismos.
US7247461B2 (en) * 1999-01-14 2007-07-24 Martek Biosciences Corporation Nucleic acid molecule encoding ORFA of a PUFA polyketide synthase system and uses thereof
US8003772B2 (en) 1999-01-14 2011-08-23 Martek Biosciences Corporation Chimeric PUFA polyketide synthase systems and uses thereof
FR2791699B1 (fr) * 1999-03-29 2004-10-29 Meristem Therapeutics Promoteurs chimeriques d'expression, cassettes d'expression, vecteurs, plantes et semences transgeniques les contenant, et methodes de leur obtention
KR20000075076A (ko) * 1999-05-28 2000-12-15 최태진 형질전환된 미세조류를 이용하여 외래 단백질을 생산하는 방법
US7625756B2 (en) 2000-06-28 2009-12-01 GycoFi, Inc. Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells
KR100787073B1 (ko) 2000-06-28 2007-12-21 글리코파이, 인크. 변형된 당단백질의 제조방법
US7795002B2 (en) 2000-06-28 2010-09-14 Glycofi, Inc. Production of galactosylated glycoproteins in lower eukaryotes
US20060257399A1 (en) 2000-06-28 2006-11-16 Glycofi, Inc. Immunoglobulins comprising predominantly a Man5GIcNAc2 glycoform
US20060029604A1 (en) 2000-06-28 2006-02-09 Gerngross Tillman U Immunoglobulins comprising predominantly a GlcNAc2Man3GlcNAc2 glycoform
ES2343106T3 (es) * 2000-09-28 2010-07-23 BIORIGINAL FOOD & SCIENCE CORP. Fad4, fad5, fad5-2 y fad6, miembros de la familia de desaturasas de acidos grasos y usos de los mismos.
US6419425B1 (en) * 2001-02-28 2002-07-16 Neu Transf'air Granular material distributing apparatus comprising at least two transfer vessels that operate in alternation
TWI324181B (en) * 2001-04-16 2010-05-01 Martek Biosciences Corp Product and process for transformation of thraustochytriales microorganisms
US7332299B2 (en) 2003-02-20 2008-02-19 Glycofi, Inc. Endomannosidases in the modification of glycoproteins in eukaryotes
MXPA05012542A (es) 2003-06-05 2006-02-08 Wyeth Corp Composiciones inmunogenicas que comprenden vectores de replicon del virus de la encefalitis equina venezolana y antigenos de la proteina del paramixovirus.
JP4796786B2 (ja) 2005-04-28 2011-10-19 富士フイルム株式会社 ラビリンチュラ類を形質転換可能なベクター
JP4796787B2 (ja) 2005-04-28 2011-10-19 富士フイルム株式会社 ラビリンチュラ類への遺伝子導入法
PT103331A (pt) 2005-08-05 2007-02-28 Fundacao Da Faculdade De Cienc Expressão dum transportador activo de xilose em saccharomyces cerevisiae modificada geneticamente
MX340759B (es) 2006-03-15 2015-03-11 Dsm Ip Assets B V * Aceites de semillas vegetales que contienen ácidos grasos poli-insaturados.
EP2082053B1 (en) 2006-08-01 2016-04-13 DSM Nutritional Products AG Process for producing microbial oil comprising polyunsaturated fatty acids
CA2615372A1 (en) 2007-07-13 2009-01-13 Marc-Andre D'aoust Influenza virus-like particles (vlps) comprising hemagglutinin
NZ598199A (en) 2007-09-12 2013-12-20 Martek Biosciences Corp Biological oils and production and uses thereof
EP2090648A1 (en) 2008-02-12 2009-08-19 Institut Francais de Recherche pour l'Exploitation de la Mere(Ifremer) Production of glycosylated polypeptides in microalgae
EP2265724A4 (en) 2008-04-09 2013-01-23 Solazyme Inc Direct chemical modification of microbial biomass and microbial oils
CN103068965A (zh) 2009-12-28 2013-04-24 Dsmip资产公司 在蔗糖上生长的重组破囊壶菌和其组合物、制备方法及用途
IN2012DN06278A (enExample) 2009-12-28 2015-09-25 Dsm Ip Assets Bv
CN107746869A (zh) 2009-12-28 2018-03-02 赛诺菲疫苗技术公司 微藻中异源多肽的生产，微藻胞外体、组合物及其制备方法和用途
US20110195448A1 (en) 2009-12-28 2011-08-11 James Casey Lippmeier Recombinant Thraustochytrids that Grow on Xylose, and Compositions, Methods of Making, and Uses Thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4381897A (en) * 1980-10-06 1983-05-03 Krupp Polysius Ag Installation for transporting fine-grained material

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
杜冰.发酵法生产DHA的研究.天津轻工业学院学报 4.2000,(4),28-30. *

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