CA2443908C - Product and process for transformation of thraustochytriales microorganisms - Google Patents

Product and process for transformation of thraustochytriales microorganisms Download PDF

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Publication number
CA2443908C
CA2443908C CA2443908A CA2443908A CA2443908C CA 2443908 C CA2443908 C CA 2443908C CA 2443908 A CA2443908 A CA 2443908A CA 2443908 A CA2443908 A CA 2443908A CA 2443908 C CA2443908 C CA 2443908C
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Canada
Prior art keywords
nucleic acid
acid sequence
seq
thraustochytriales
protein
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Expired - Fee Related
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CA2443908A
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English (en)
French (fr)
Other versions
CA2443908A1 (en
Inventor
Paul G. Roessler
T. Dave Matthews
Tom M. Ramseier
James G. Metz
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DSM IP Assets BV
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DSM IP Assets BV
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Publication of CA2443908C publication Critical patent/CA2443908C/en
Anticipated expiration legal-status Critical
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y202/00—Transferases transferring aldehyde or ketonic groups (2.2)
    • C12Y202/01—Transketolases and transaldolases (2.2.1)
    • C12Y202/01006—Acetolactate synthase (2.2.1.6)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Mushroom Cultivation (AREA)
  • Enzymes And Modification Thereof (AREA)
CA2443908A 2001-04-16 2002-04-16 Product and process for transformation of thraustochytriales microorganisms Expired - Fee Related CA2443908C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US28411601P 2001-04-16 2001-04-16
US60/284,116 2001-04-16
PCT/US2002/012040 WO2002083869A2 (en) 2001-04-16 2002-04-16 Product and process for transformation of thraustochytriales microorganisms

Publications (2)

Publication Number Publication Date
CA2443908A1 CA2443908A1 (en) 2002-10-24
CA2443908C true CA2443908C (en) 2016-01-12

Family

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Family Applications (1)

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CA2443908A Expired - Fee Related CA2443908C (en) 2001-04-16 2002-04-16 Product and process for transformation of thraustochytriales microorganisms

Country Status (10)

Country Link
US (5) US7001772B2 (enExample)
EP (3) EP2314706B1 (enExample)
JP (4) JP4536324B2 (enExample)
CN (3) CN100513564C (enExample)
AT (1) ATE533844T1 (enExample)
AU (1) AU2002250592C1 (enExample)
CA (1) CA2443908C (enExample)
ES (3) ES2377557T3 (enExample)
TW (3) TWI324181B (enExample)
WO (1) WO2002083869A2 (enExample)

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