JP6211577B2 - トラウストチトリアレス微生物の形質転換についての産物および方法 - Google Patents
トラウストチトリアレス微生物の形質転換についての産物および方法 Download PDFInfo
- Publication number
- JP6211577B2 JP6211577B2 JP2015253726A JP2015253726A JP6211577B2 JP 6211577 B2 JP6211577 B2 JP 6211577B2 JP 2015253726 A JP2015253726 A JP 2015253726A JP 2015253726 A JP2015253726 A JP 2015253726A JP 6211577 B2 JP6211577 B2 JP 6211577B2
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- seq
- protein
- acid sequence
- schizochytrium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y202/00—Transferases transferring aldehyde or ketonic groups (2.2)
- C12Y202/01—Transketolases and transaldolases (2.2.1)
- C12Y202/01006—Acetolactate synthase (2.2.1.6)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Mushroom Cultivation (AREA)
- Enzymes And Modification Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Description
本発明は、一般的に、トラウストチトリアレス目(Thraustochytriales)の微生物にスルホニル尿素化合物、イミダゾリノン類阻害剤および/またはピリミジニルオキシ安息香酸に対する低下した感受性を与えるアセト乳酸シンターゼを含む、トラウストチトリアレスのアセト乳酸シンターゼをコードする単離された核酸分子;トラウストチトリアレス目の微生物の形質転換のために有用な選択マーカーを含む組換え核酸分子、ならびに本発明の組換え核酸分子を用いてそのような微生物を形質転換する方法に関する。本発明はまた、トラウストチトリアレス発現系において有用な遺伝子プロモーターに関する。本発明の組換え核酸分子は、トラウストチトリアレス微生物において外来核酸の発現、およびトラウストチトリアレス微生物における遺伝子の欠失、突然変異または不活化のために用いられうる。
開発することによりトラウストチトリド(Thraustochytrid)の分類の改正をもたらした。分類学理論家は、トラウストチトリドを藻類または藻類様原生生物と定める。しかしながら、分類の不確定性のため、本発明に記載される系統をトラウストチトリド(目:トラウストチトリアレス;科:トラウストチトリアシエ(Thraustochytriaceae);属:トラウストチトリウム(Thraustochytrium)、シゾチトリウム(Schizochytrium)、ラビリンチュロイデス(Labyrinthuloides)またはジャポノチトリウム(Japonochytrium))とみなすことが本発明の目的にとって最善であるものと思われる。分類変更は下記に要約されている。
界:有色植物界(ストラメノパイル)
門:不等毛門(Heterokonta)
目:トラウストチトリアレス
科:トラウストチトリアシエ
属:トラウストチトリウム、シゾチトリウム、ラビリンチュロイデス、またはジャポノチトリウム
本発明は、トラウストチトリアレス目の微生物を遺伝的に形質転換するための方法および関連した材料を含む。本発明の組換え構築物の形質転換体としての使用のために本明細書に開示される単細胞微生物のすべての株は、一般的にトラウストチトリドとも呼ばれうるのだが、トラウストチトリアレス目のメンバーである。本発明により、句「トラウストチトリド」、「トラウストチトリアレス微生物」および「トラウストチトリアレス目の微生物」は、交換可能に用いられうる。本発明者らは、シゾチトリウムまたは他のいずれのトラウストチトリアレス微生物についての形質転換系を記載する先行報告を一つも認識していない。本明細書に記載される形質転換系は、外来遺伝子をトラウストチトリアレス目の微生物へ導入し、それにより高められた商業的価値をもつ系統を作製するための手段を提供するために用いられうる。さらに、本発明は、相同的または非相同的組換えによる特定の遺伝子の変異または不活化を可能にし、細胞の代謝を変えるための、およびトラウストチトリアレス微生物における特定の遺伝子の機能を同定するための新規な方法を提供する。
一致についての報酬 = 1
不一致についてのペナルティ = -2
オープンギャップ(5)および伸長ギャップ(2)ペナルティ
ギャップx_ドロップオフ(50)期待(10)ワードサイズ(11)フィルター(オン)
0 BLOSUM62行列を用いる、blastpについて:
オープンギャップ(11)および伸長ギャップ(1)ペナルティ
ギャップx_ドロップオフ(50)期待(10)ワードサイズ(3)フィルター(オン)。
この実施例は、組換えプラスミドpTUBZEO 11-2の作製を記載する。
この実施例は、組換えプラスミドpMON50200、pMON50201、pMON50202、およびpMON50203の作製を記載する。
この実施例は、実施例1および実施例2に記載される組換え分子でのシゾチトリウム属種の遺伝的形質転換を記載する。
以下の実施例は、形質転換されたシゾチトリウム細胞のPCR分析を実証する。
以下の実施例は、形質転換されたシゾチトリウム細胞のサザンブロット分析を記載する。
この実施例は、シゾチトリウムにおける相同的組換えを実証している。
この実施例は、選択マーカー遺伝子に連結されていない追加の外来DNA分子を含む株を同時形質転換により作製するために形質転換ベクターpTUBZEO 11-2またはpMON50202の使用を記載する。
Claims (5)
- 以下の工程を含む、タンパク質の生産のための方法:
シゾチトリウム属の組換え微生物を培地中で培養する工程であって、該組換え微生物が、タンパク質を生産するために、タンパク質をコードする外来遺伝子を含む単離された核酸分子を含む、工程;および
タンパク質を回収する工程、
ここで、単離された核酸分子が1つまたは複数のシゾチトリウムのプロモーター配列をさらに含み、かつ外来遺伝子が1つまたは複数のシゾチトリウムのプロモーター配列に実施可能に連結される方法。 - シゾチトリウムのプロモーター配列が、シゾチトリウムのアセト乳酸シンターゼプロモーター配列、シゾチトリウムのα-チューブリンプロモーター配列、シゾチトリウムのポリケチドシンターゼ系プロモーター配列、およびシゾチトリウムの脂肪酸デサチュラーゼプロモーター配列からなる群より選択される、請求項1記載の方法。
- 単離された核酸分子がシグナルセグメント核酸配列をさらに含む、請求項1記載の方法。
- タンパク質が培地中に分泌され、タンパク質が培地から回収される、請求項1記載の方法。
- 微生物が、シゾチトリウム属種、シゾチトリウムアグレガタム、シゾチトリウムリマシナム、およびシゾチトリウムミヌタムからなる群より選択される種由来のものである、請求項1記載の方法。
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US28411601P | 2001-04-16 | 2001-04-16 | |
US60/284,116 | 2001-04-16 |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2012215905A Division JP2013005817A (ja) | 2001-04-16 | 2012-09-28 | トラウストチトリアレス微生物の形質転換についての産物および方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2016039831A JP2016039831A (ja) | 2016-03-24 |
JP6211577B2 true JP6211577B2 (ja) | 2017-10-11 |
Family
ID=23088908
Family Applications (4)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2002582208A Expired - Fee Related JP4536324B2 (ja) | 2001-04-16 | 2002-04-16 | トラウストチトリアレス微生物の形質転換についての産物および方法 |
JP2010019881A Expired - Lifetime JP5248536B2 (ja) | 2001-04-16 | 2010-02-01 | トラウストチトリアレス微生物の形質転換についての産物および方法 |
JP2012215905A Withdrawn JP2013005817A (ja) | 2001-04-16 | 2012-09-28 | トラウストチトリアレス微生物の形質転換についての産物および方法 |
JP2015253726A Expired - Fee Related JP6211577B2 (ja) | 2001-04-16 | 2015-12-25 | トラウストチトリアレス微生物の形質転換についての産物および方法 |
Family Applications Before (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2002582208A Expired - Fee Related JP4536324B2 (ja) | 2001-04-16 | 2002-04-16 | トラウストチトリアレス微生物の形質転換についての産物および方法 |
JP2010019881A Expired - Lifetime JP5248536B2 (ja) | 2001-04-16 | 2010-02-01 | トラウストチトリアレス微生物の形質転換についての産物および方法 |
JP2012215905A Withdrawn JP2013005817A (ja) | 2001-04-16 | 2012-09-28 | トラウストチトリアレス微生物の形質転換についての産物および方法 |
Country Status (11)
Country | Link |
---|---|
US (5) | US7001772B2 (ja) |
EP (3) | EP1414941B1 (ja) |
JP (4) | JP4536324B2 (ja) |
CN (3) | CN100513564C (ja) |
AT (1) | ATE533844T1 (ja) |
AU (1) | AU2002250592C1 (ja) |
CA (1) | CA2443908C (ja) |
ES (3) | ES2688953T3 (ja) |
HK (2) | HK1065819A1 (ja) |
TW (3) | TWI324181B (ja) |
WO (1) | WO2002083869A2 (ja) |
Families Citing this family (78)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6566583B1 (en) * | 1997-06-04 | 2003-05-20 | Daniel Facciotti | Schizochytrium PKS genes |
US20070244192A1 (en) * | 1999-01-14 | 2007-10-18 | Martek Biosciences Corporation | Plant seed oils containing polyunsaturated fatty acids |
US8003772B2 (en) | 1999-01-14 | 2011-08-23 | Martek Biosciences Corporation | Chimeric PUFA polyketide synthase systems and uses thereof |
US7247461B2 (en) * | 1999-01-14 | 2007-07-24 | Martek Biosciences Corporation | Nucleic acid molecule encoding ORFA of a PUFA polyketide synthase system and uses thereof |
US7211418B2 (en) * | 1999-01-14 | 2007-05-01 | Martek Biosciences Corporation | PUFA polyketide synthase systems and uses thereof |
US7217856B2 (en) * | 1999-01-14 | 2007-05-15 | Martek Biosciences Corporation | PUFA polyketide synthase systems and uses thereof |
TWI324181B (en) * | 2001-04-16 | 2010-05-01 | Martek Biosciences Corp | Product and process for transformation of thraustochytriales microorganisms |
US7211656B2 (en) * | 2002-01-30 | 2007-05-01 | Abbott Laboratories | Desaturase genes, enzymes encoded thereby, and uses thereof |
WO2004056986A2 (en) * | 2002-12-20 | 2004-07-08 | Chromagenics B.V. | Means and methods for producing a protein through chromatin openers that are capable of rendering chromatin more accessible to transcription factors |
US7208590B2 (en) * | 2003-07-15 | 2007-04-24 | Abbott Laboratories | Genes involved in polyketide synthase pathways and uses thereof |
BRPI0510132A (pt) | 2004-04-22 | 2007-10-02 | Commw Scient Ind Res Org | sìntese de ácidos graxos poliinsaturados de cadeia longa por células recombinantes |
CN102559364B (zh) | 2004-04-22 | 2016-08-17 | 联邦科学技术研究组织 | 用重组细胞合成长链多不饱和脂肪酸 |
MX2007004595A (es) * | 2004-11-08 | 2007-08-15 | Chromagenics Bv | Seleccion de celulas hospederas que expresan proteinas en altas concentraciones. |
US8999667B2 (en) * | 2004-11-08 | 2015-04-07 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
US20100136616A1 (en) * | 2004-11-08 | 2010-06-03 | Chromagenics B.V. | Selection of Host Cells Expressing Protein at High Levels |
US20060195935A1 (en) | 2004-11-08 | 2006-08-31 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
US20060172382A1 (en) * | 2004-11-08 | 2006-08-03 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
JP4796787B2 (ja) | 2005-04-28 | 2011-10-19 | 富士フイルム株式会社 | ラビリンチュラ類への遺伝子導入法 |
JP4796786B2 (ja) * | 2005-04-28 | 2011-10-19 | 富士フイルム株式会社 | ラビリンチュラ類を形質転換可能なベクター |
ES2576986T3 (es) | 2005-06-07 | 2016-07-12 | Dsm Nutritional Products Ag | Microorganismos eucariotas para la producción de lípidos y antioxidantes |
DK1951866T3 (da) | 2005-11-23 | 2014-10-27 | Du Pont | Delta-9-elongaser og anvendelse heraf til fremstilling af flerumættede fedtsyrer |
EP1818411A1 (en) * | 2006-02-13 | 2007-08-15 | Lonza AG | Process for the preparation of optically active chiral amines |
JP2009529891A (ja) * | 2006-03-15 | 2009-08-27 | マーテック バイオサイエンシーズ コーポレーション | 多価不飽和脂肪酸を含む植物種子油 |
KR20090007407A (ko) * | 2006-04-11 | 2009-01-16 | 마텍 바이오싸이언스스 코포레이션 | 장쇄 다중불포화 지방산을 포함하는 식품 제품 및 그의 제조 방법 |
AU2007260873B2 (en) | 2006-06-22 | 2013-05-02 | Dsm Ip Assets B.V. | Encapsulated labile compound compositions and methods of making the same |
US9023616B2 (en) * | 2006-08-01 | 2015-05-05 | Dsm Nutritional Products Ag | Oil producing microbes and method of modification thereof |
SG10201501004UA (en) | 2006-08-29 | 2015-04-29 | Dsm Ip Assets Bv | USE OF DPA(n-6) OILS IN INFANT FORMULA |
EP2059588A4 (en) | 2006-08-29 | 2010-07-28 | Commw Scient Ind Res Org | FATTY ACID SYNTHESIS |
KR100860111B1 (ko) * | 2006-10-02 | 2008-09-25 | 인제대학교 산학협력단 | 신규 미생물 트라우스토카이트리움 에스피.케이제이에스-1, 바실러스 폴리퍼멘티쿠스 케이제이에스-2 및 이들과 바실러스 리케니포르미스를 포함하고 있는 사료첨가제 및 사료 |
AU2007322223B2 (en) | 2006-10-23 | 2012-12-13 | Corteva Agriscience Llc | Delta-8 desaturases and their use in making polyunsaturated fatty acids |
US8188338B2 (en) | 2007-04-10 | 2012-05-29 | E. I. Du Pont De Nemours And Company | Delta-8 desaturases and their use in making polyunsaturated fatty acids |
US8119860B2 (en) | 2007-04-16 | 2012-02-21 | E. I. Du Pont De Nemours And Company | Delta-9 elongases and their use in making polyunsaturated fatty acids |
US7943365B2 (en) | 2007-05-03 | 2011-05-17 | E.I. Du Pont De Nemours And Company | Δ-5 desaturases and their use in making polyunsaturated fatty acids |
JP2010532418A (ja) * | 2007-06-29 | 2010-10-07 | マーテック バイオサイエンシーズ コーポレーション | 多価不飽和脂肪酸のエステルの製造方法および精製方法 |
NZ598199A (en) | 2007-09-12 | 2013-12-20 | Martek Biosciences Corp | Biological oils and production and uses thereof |
US20110076379A1 (en) * | 2008-06-13 | 2011-03-31 | Means Michael M | High omega saturated fat free meat products |
CN114045301A (zh) | 2008-11-18 | 2022-02-15 | 联邦科学技术研究组织 | 产生ω-3脂肪酸的酶和方法 |
WO2010080388A1 (en) | 2008-12-18 | 2010-07-15 | E. I. Du Pont De Nemours And Company | Reducing byproduction of malonates in a fermentation process |
JP5415896B2 (ja) | 2009-01-29 | 2014-02-12 | Jfeスチール株式会社 | インダイレクトスポット溶接方法 |
ES2752196T3 (es) * | 2009-03-16 | 2020-04-03 | Dsm Ip Assets Bv | Producción de proteínas en microorganismos del filo Labyrinthulomycota |
EP3192871B1 (en) * | 2009-03-19 | 2019-01-23 | DSM IP Assets B.V. | Polyunsaturated fatty acid synthase nucleic acid molecules and polypeptides, compositions, and methods of making and uses thereof |
US8207363B2 (en) | 2009-03-19 | 2012-06-26 | Martek Biosciences Corporation | Thraustochytrids, fatty acid compositions, and methods of making and uses thereof |
AU2010260227B2 (en) | 2009-06-16 | 2015-01-29 | E. I. Du Pont De Nemours And Company | Improvement of long chain omega-3 and omega-6 polyunsaturated fatty acid biosynthesis by expression of acyl-CoA lysophospholipid acyltransferases |
CN102725405B (zh) * | 2009-09-24 | 2015-08-19 | 国立大学法人九州大学 | 原生藻菌的转化方法 |
PE20121685A1 (es) | 2009-12-28 | 2012-12-28 | Merial Ltd | Antigeno ndv recombinante y usos del mismo |
WO2011082190A1 (en) | 2009-12-28 | 2011-07-07 | Martek Biosciences Corporation | Recombinant thraustochytrids that grow on sucrose, and compositions, methods of making, and uses thereof |
CN102906270B (zh) * | 2009-12-28 | 2016-06-22 | Dsmip资产公司 | 在木糖上生长的重组破囊壶菌和其组合物、制备方法及用途 |
AU2010339544B2 (en) * | 2009-12-28 | 2015-11-12 | Merial Limited | Production of hemagglutinin-neuraminidase protein in microalgae |
US20110189228A1 (en) * | 2009-12-28 | 2011-08-04 | Bayne Anne-Cecile V | Production of Heterologous Polypeptides in Microalgae, Microalgal Extracellular Bodies, Compositions, and Methods of Making and Uses Thereof |
CN102884201B (zh) | 2010-01-19 | 2016-04-13 | 帝斯曼知识产权资产管理有限公司 | 生产二十碳五烯酸的微生物、脂肪酸组成物及其制作方法与用途 |
EP2944322B1 (en) | 2010-03-12 | 2018-01-17 | Merial, Inc | Bluetongue virus recombinant vaccines and uses thereof |
US8980589B2 (en) | 2010-08-26 | 2015-03-17 | E I Du Pont De Nemours And Company | Mutant delta-9 elongases and their use in making polyunsaturated fatty acids |
CN103080317B (zh) | 2010-08-26 | 2017-07-11 | 纳幕尔杜邦公司 | 突变型hpgg基序和hdash基序δ‑5去饱和酶以及它们在制备多不饱和脂肪酸中的用途 |
AU2012226528B2 (en) | 2011-03-07 | 2015-08-20 | Dsm Nutritional Products Ag | Engineering thraustochytrid microorganisms |
CN108771240A (zh) | 2011-07-21 | 2018-11-09 | 帝斯曼知识产权资产管理有限公司 | 脂肪酸组合物 |
US8816111B2 (en) | 2012-06-15 | 2014-08-26 | Commonwealth Scientific And Industrial Research Organisation | Lipid comprising polyunsaturated fatty acids |
BR112014031827A2 (pt) | 2012-06-19 | 2017-06-27 | Du Pont | produção aprimorada de ácidos graxos poli-insaturados por coexpressão de acil- coa: lisofosfatidilcolina aciltransferases e fosfolipídeo: diacilglicerol aciltransferases. |
AU2014229307B2 (en) | 2013-03-13 | 2017-08-31 | Dsm Nutritional Products Ag | Engineering microorganisms |
CN104726473B (zh) | 2013-12-18 | 2020-02-14 | 联邦科学技术研究组织 | 包含二十二碳六烯酸的提取的植物脂质 |
CN105219789B (zh) | 2014-06-27 | 2023-04-07 | 联邦科学技术研究组织 | 包含二十二碳五烯酸的提取的植物脂质 |
CN105567715B (zh) * | 2014-10-17 | 2021-06-29 | 丰益(上海)生物技术研发中心有限公司 | 裂殖壶菌α-微管蛋白相关序列及其应用 |
HUE051749T2 (hu) | 2015-03-02 | 2021-03-29 | Conagen Inc | Labirintusgomba mikroorganizmusokból származó szabályozó elemek |
AR104042A1 (es) | 2015-03-26 | 2017-06-21 | Mara Renewables Corp | Producción de alta densidad de biomasa y aceite utilizando glicerol en bruto |
PL3302032T3 (pl) | 2015-06-06 | 2022-06-20 | Dsm Ip Assets B.V. | Wytwarzanie wielonienasyconych kwasów tłuszczowych (PUFA) z zastosowaniem nowej, modularnej syntazy kwasu dokozaheksaenowego (DHA) |
US10542961B2 (en) | 2015-06-15 | 2020-01-28 | The Research Foundation For The State University Of New York | System and method for infrasonic cardiac monitoring |
BR112018000690A2 (pt) | 2015-07-13 | 2018-09-18 | MARA Renewables Corporation | micro-organismo recombinante, e, métodos para preparar um micro-organismo de metabolização de xilose e para produzir óleo. |
IL241462A0 (en) | 2015-09-10 | 2015-11-30 | Yeda Res & Dev | Heterologous engineering of betalain pigments in plants |
CA3017225A1 (en) | 2016-03-16 | 2017-09-21 | Synthetic Genomics, Inc. | Production of proteins in labyrinthulomycetes |
KR102442450B1 (ko) | 2016-05-12 | 2022-09-14 | 디에스엠 아이피 어셋츠 비.브이. | 미세조류에서 오메가-3 다중불포화 지방산 생산을 증가시키는 방법 |
US10851395B2 (en) | 2016-06-10 | 2020-12-01 | MARA Renewables Corporation | Method of making lipids with improved cold flow properties |
CN109689856A (zh) | 2016-07-13 | 2019-04-26 | 帝斯曼知识产权资产管理有限公司 | 用于海藻宿主细胞的CRISPR-Cas系统 |
IL247752A0 (en) | 2016-09-11 | 2016-11-30 | Yeda Res & Dev | Compositions and methods for modulating gene expression for site-directed mutagenesis |
US10633454B2 (en) | 2016-11-01 | 2020-04-28 | Conagen Inc. | Expression of modified glycoproteins and glycopeptides |
WO2019078359A1 (ja) | 2017-10-19 | 2019-04-25 | 味の素株式会社 | ステロールの製造法 |
EP3762485A4 (en) * | 2018-03-05 | 2021-12-08 | Conagen Inc. | ORGANISMS AND PROCESSES FOR THE PRODUCTION OF LOW SULPHATION GLYCOMOLECULES |
EP3788164A4 (en) * | 2018-05-01 | 2022-01-26 | Conagen Inc. | RECOMBINANT ORGANISMS AND METHODS FOR PRODUCTION OF HIGH GLYCAN OCCUPANCY GLYCOMOLECULES |
EP3835410A4 (en) * | 2018-08-10 | 2022-05-18 | Kyowa Hakko Bio Co., Ltd. | EICOSAPENTAIC ACID PRODUCING MICROORGANISM AND PROCESS FOR PRODUCTION OF EICOSAPENTAIC ACID |
CN109777815B (zh) * | 2019-03-28 | 2021-10-29 | 昆明理工大学 | HMG-CoA合成酶基因RKHMGCS及其应用 |
Family Cites Families (49)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BE545283A (ja) | ||||
LU35839A1 (ja) | ||||
US3223456A (en) * | 1963-11-14 | 1965-12-14 | Siemens Ag | Conveying apparatus for fine-granular material |
US4381897A (en) * | 1980-10-06 | 1983-05-03 | Krupp Polysius Ag | Installation for transporting fine-grained material |
US4626505A (en) | 1984-02-24 | 1986-12-02 | E. I. Du Pont De Nemours And Company | Selectable markers for yeast transformation |
IL83348A (en) * | 1986-08-26 | 1995-12-08 | Du Pont | Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase |
US5605011A (en) * | 1986-08-26 | 1997-02-25 | E. I. Du Pont De Nemours And Company | Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase |
US5340742A (en) | 1988-09-07 | 1994-08-23 | Omegatech Inc. | Process for growing thraustochytrium and schizochytrium using non-chloride salts to produce a microfloral biomass having omega-3-highly unsaturated fatty acids |
US5130242A (en) * | 1988-09-07 | 1992-07-14 | Phycotech, Inc. | Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids |
US5426040A (en) * | 1989-08-17 | 1995-06-20 | Northeastern University | Methods for producing improved strains of seaweed by fusion of spore-protoplasts, and resultant seaweeds and phycocolloids |
IL100908A0 (en) | 1991-02-22 | 1992-11-15 | Salk Inst Biotech Ind | Invertase genes and their use |
US5804408A (en) | 1991-03-13 | 1998-09-08 | Yoshihide Hagiwara | Expression of human SOD in blue green algae |
US5661017A (en) * | 1993-09-14 | 1997-08-26 | Dunahay; Terri Goodman | Method to transform algae, materials therefor, and products produced thereby |
IL113375A (en) * | 1995-04-13 | 1997-09-30 | Fortress U & T Ltd | Internationally regulated system for one to one cryptographic communications with national sovereignty without key escrow |
US5853973A (en) * | 1995-04-20 | 1998-12-29 | American Cyanamid Company | Structure based designed herbicide resistant products |
EP0821729B1 (en) * | 1995-04-20 | 2006-10-18 | Basf Aktiengesellschaft | Structure-based designed herbicide resistant products |
DE19529475A1 (de) * | 1995-08-11 | 1997-02-13 | Marcus Weis | Siebkonstruktion zum Abtrennen des Faseranteils aus der Shredderleichtfraktion |
US5824309A (en) | 1995-12-05 | 1998-10-20 | University Of Massachusetts | Recombinant gas vesicles and uses thereof |
WO1997039106A1 (en) * | 1996-04-12 | 1997-10-23 | Martek Biosciences Corporation | Methods and tools for transformation of eukaryotic algae |
US6194167B1 (en) * | 1997-02-18 | 2001-02-27 | Washington State University Research Foundation | ω-3 fatty acid desaturase |
US5968809A (en) * | 1997-04-11 | 1999-10-19 | Abbot Laboratories | Methods and compositions for synthesis of long chain poly-unsaturated fatty acids |
ES2125195B1 (es) | 1997-04-18 | 1999-10-01 | Antibioticos Sa | Procedimiento de inactivacion de genes que codifican para enzimas del catabolismo del fenilacetato, plasmidos que intervienen y cepas transformadas con los mismos. |
US8003772B2 (en) | 1999-01-14 | 2011-08-23 | Martek Biosciences Corporation | Chimeric PUFA polyketide synthase systems and uses thereof |
US7247461B2 (en) * | 1999-01-14 | 2007-07-24 | Martek Biosciences Corporation | Nucleic acid molecule encoding ORFA of a PUFA polyketide synthase system and uses thereof |
FR2791699B1 (fr) * | 1999-03-29 | 2004-10-29 | Meristem Therapeutics | Promoteurs chimeriques d'expression, cassettes d'expression, vecteurs, plantes et semences transgeniques les contenant, et methodes de leur obtention |
KR20000075076A (ko) * | 1999-05-28 | 2000-12-15 | 최태진 | 형질전환된 미세조류를 이용하여 외래 단백질을 생산하는 방법 |
US20060257399A1 (en) | 2000-06-28 | 2006-11-16 | Glycofi, Inc. | Immunoglobulins comprising predominantly a Man5GIcNAc2 glycoform |
US7625756B2 (en) | 2000-06-28 | 2009-12-01 | GycoFi, Inc. | Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells |
DE60139720D1 (de) | 2000-06-28 | 2009-10-08 | Glycofi Inc | Verfahren für die Herstellung modifizierter Glykoproteine |
US7795002B2 (en) | 2000-06-28 | 2010-09-14 | Glycofi, Inc. | Production of galactosylated glycoproteins in lower eukaryotes |
US20060029604A1 (en) | 2000-06-28 | 2006-02-09 | Gerngross Tillman U | Immunoglobulins comprising predominantly a GlcNAc2Man3GlcNAc2 glycoform |
ATE510908T1 (de) * | 2000-09-28 | 2011-06-15 | Bioriginal Food & Science Corp | Fad5-2, mitglied der desaturase-familie und verwendungen davon |
US6419425B1 (en) * | 2001-02-28 | 2002-07-16 | Neu Transf'air | Granular material distributing apparatus comprising at least two transfer vessels that operate in alternation |
TWI324181B (en) * | 2001-04-16 | 2010-05-01 | Martek Biosciences Corp | Product and process for transformation of thraustochytriales microorganisms |
US7332299B2 (en) | 2003-02-20 | 2008-02-19 | Glycofi, Inc. | Endomannosidases in the modification of glycoproteins in eukaryotes |
ATE404582T1 (de) | 2003-06-05 | 2008-08-15 | Wyeth Corp | Immunogene zusammensetzungen, die replikonvektoren des venezolanischen pferdeencephalitisvirus und paramyxovirus- proteinantigene enthalten |
JP4796787B2 (ja) | 2005-04-28 | 2011-10-19 | 富士フイルム株式会社 | ラビリンチュラ類への遺伝子導入法 |
JP4796786B2 (ja) | 2005-04-28 | 2011-10-19 | 富士フイルム株式会社 | ラビリンチュラ類を形質転換可能なベクター |
PT103331A (pt) | 2005-08-05 | 2007-02-28 | Fundacao Da Faculdade De Cienc | Expressão dum transportador activo de xilose em saccharomyces cerevisiae modificada geneticamente |
JP2009529891A (ja) | 2006-03-15 | 2009-08-27 | マーテック バイオサイエンシーズ コーポレーション | 多価不飽和脂肪酸を含む植物種子油 |
US9023616B2 (en) | 2006-08-01 | 2015-05-05 | Dsm Nutritional Products Ag | Oil producing microbes and method of modification thereof |
CA2615372A1 (en) | 2007-07-13 | 2009-01-13 | Marc-Andre D'aoust | Influenza virus-like particles (vlps) comprising hemagglutinin |
NZ598199A (en) | 2007-09-12 | 2013-12-20 | Martek Biosciences Corp | Biological oils and production and uses thereof |
EP2090648A1 (en) | 2008-02-12 | 2009-08-19 | Institut Francais de Recherche pour l'Exploitation de la Mere(Ifremer) | Production of glycosylated polypeptides in microalgae |
AU2009234176B2 (en) | 2008-04-09 | 2014-08-07 | Corbion Biotech, Inc. | Direct chemical modification of microbial biomass and microbial oils |
CN102906270B (zh) | 2009-12-28 | 2016-06-22 | Dsmip资产公司 | 在木糖上生长的重组破囊壶菌和其组合物、制备方法及用途 |
WO2011082190A1 (en) | 2009-12-28 | 2011-07-07 | Martek Biosciences Corporation | Recombinant thraustochytrids that grow on sucrose, and compositions, methods of making, and uses thereof |
AU2010339544B2 (en) | 2009-12-28 | 2015-11-12 | Merial Limited | Production of hemagglutinin-neuraminidase protein in microalgae |
US20110189228A1 (en) | 2009-12-28 | 2011-08-04 | Bayne Anne-Cecile V | Production of Heterologous Polypeptides in Microalgae, Microalgal Extracellular Bodies, Compositions, and Methods of Making and Uses Thereof |
-
2002
- 2002-04-15 TW TW091107634A patent/TWI324181B/zh not_active IP Right Cessation
- 2002-04-15 TW TW098130758A patent/TWI350854B/zh not_active IP Right Cessation
- 2002-04-15 TW TW100112656A patent/TWI377253B/zh not_active IP Right Cessation
- 2002-04-16 WO PCT/US2002/012040 patent/WO2002083869A2/en active Search and Examination
- 2002-04-16 AT AT02719515T patent/ATE533844T1/de active
- 2002-04-16 JP JP2002582208A patent/JP4536324B2/ja not_active Expired - Fee Related
- 2002-04-16 ES ES10178886.7T patent/ES2688953T3/es not_active Expired - Lifetime
- 2002-04-16 US US10/124,807 patent/US7001772B2/en not_active Expired - Lifetime
- 2002-04-16 AU AU2002250592A patent/AU2002250592C1/en not_active Ceased
- 2002-04-16 ES ES02719515T patent/ES2377557T3/es not_active Expired - Lifetime
- 2002-04-16 CN CNB2006100842683A patent/CN100513564C/zh not_active Expired - Fee Related
- 2002-04-16 CN CN200910141898A patent/CN101760469A/zh active Pending
- 2002-04-16 EP EP02719515A patent/EP1414941B1/en not_active Expired - Lifetime
- 2002-04-16 CN CN028120590A patent/CN1556850B/zh not_active Expired - Fee Related
- 2002-04-16 ES ES10178911T patent/ES2698473T3/es not_active Expired - Lifetime
- 2002-04-16 EP EP10178886.7A patent/EP2325319B1/en not_active Expired - Lifetime
- 2002-04-16 EP EP10178911.3A patent/EP2314706B1/en not_active Expired - Lifetime
- 2002-04-16 CA CA2443908A patent/CA2443908C/en not_active Expired - Fee Related
-
2004
- 2004-11-02 HK HK04108611.8A patent/HK1065819A1/xx not_active IP Right Cessation
- 2004-12-22 US US11/021,712 patent/US8206984B2/en not_active Expired - Fee Related
-
2007
- 2007-01-22 HK HK07100765.6A patent/HK1094711A1/xx not_active IP Right Cessation
- 2007-10-27 US US11/925,885 patent/US7851191B2/en not_active Expired - Fee Related
-
2010
- 2010-02-01 JP JP2010019881A patent/JP5248536B2/ja not_active Expired - Lifetime
- 2010-11-09 US US12/942,759 patent/US20110086390A1/en not_active Abandoned
-
2012
- 2012-09-28 JP JP2012215905A patent/JP2013005817A/ja not_active Withdrawn
-
2013
- 2013-06-21 US US13/923,481 patent/US8945875B2/en not_active Expired - Fee Related
-
2015
- 2015-12-25 JP JP2015253726A patent/JP6211577B2/ja not_active Expired - Fee Related
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP6211577B2 (ja) | トラウストチトリアレス微生物の形質転換についての産物および方法 | |
AU2002250592A1 (en) | Product and process for transformation of thraustochytriales microorganisms | |
CN102741267B (zh) | 多不饱和脂肪酸合酶核酸分子和多肽,及其组合物、制备方法和用途 | |
CN102257126A (zh) | 减少发酵过程中的丙二酸副产物 | |
JP2006304686A (ja) | ラビリンチュラ類への遺伝子導入法 | |
JP2023507891A (ja) | リパーゼ改変株 | |
JP4803584B2 (ja) | 脂質生産性の高い形質転換微生物 | |
ES2772650T3 (es) | Procedimiento para producir 7-deshidrocolesterol y vitamina D3 | |
CN112469813A (zh) | 用于产生甲基丙烯酸酯的方法 | |
AU2007249124B2 (en) | Product and process for transformation of thraustochytriales microorganisms | |
KR20160111947A (ko) | 재조합 미생물의 생산 방법 | |
AU2012203619A1 (en) | Product and process for transformation of thraustochytriales microorganisms | |
CN111433219A (zh) | 通过基因修饰信号传导蛋白提高藻类脂质生产力 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20160122 |
|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20160122 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20161031 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20170130 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20170427 |
|
RD03 | Notification of appointment of power of attorney |
Free format text: JAPANESE INTERMEDIATE CODE: A7423 Effective date: 20170615 |
|
RD04 | Notification of resignation of power of attorney |
Free format text: JAPANESE INTERMEDIATE CODE: A7424 Effective date: 20170615 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20170815 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20170913 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 6211577 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
LAPS | Cancellation because of no payment of annual fees |