CN1395615A - 产生l－谷氨酰胺的微生物和使用该微生物产生l－谷氨酰胺的方法 - Google Patents

产生l－谷氨酰胺的微生物和使用该微生物产生l－谷氨酰胺的方法 Download PDF

Info

Publication number
CN1395615A
CN1395615A CN01803831A CN01803831A CN1395615A CN 1395615 A CN1395615 A CN 1395615A CN 01803831 A CN01803831 A CN 01803831A CN 01803831 A CN01803831 A CN 01803831A CN 1395615 A CN1395615 A CN 1395615A
Authority
CN
China
Prior art keywords
brevibacterium
glutamine
glutaminate
substratum
cjja21
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN01803831A
Other languages
English (en)
Other versions
CN1181190C (zh
Inventor
朴晟植
徐承铉
李根喆
李东雨
金天柱
郑相哲
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CJ Corp
CJ CheilJedang Corp
Original Assignee
First Sugar Refining Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR10-2000-0068284A external-priority patent/KR100397318B1/ko
Priority claimed from KR10-2000-0068285A external-priority patent/KR100397317B1/ko
Application filed by First Sugar Refining Corp filed Critical First Sugar Refining Corp
Publication of CN1395615A publication Critical patent/CN1395615A/zh
Application granted granted Critical
Publication of CN1181190C publication Critical patent/CN1181190C/zh
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/14—Glutamic acid; Glutamine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/13—Brevibacterium
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/84—Brevibacterium

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

本发明公开了新型微生物：具有叠氮钠抗性的乳发酵短杆菌CJJA21(保藏号KCCM－10222)以及具有α－氨基丁酸抗性的乳发酵短杆菌CJJA22(保藏号KCCM－10223)，这两种菌株产生L－谷氨酰胺的产率都要比已知菌株高。还公开了使用该微生物生产L－谷氨酰胺的方法。

Description

产生L-谷氨酰胺的微生物和 使用该微生物产生L-谷氨酰胺的方法
                         技术领域
本发明涉及产生L-谷氨酰胺的新微生物及使用该微生物产生L-谷氨酰胺的方法。更具体地说，本发明涉及抗叠氮化钠的乳发酵短杆菌(Brevibacterium lactofermentum)CJJA21(KCCM-10222)和抗D，L-α-氨基正丁酸(α-ABA)的乳发酵短杆菌(Brevibacteriumlactofermentum)CJJA22(KCCM-10223)，两者都能够以高于已知菌株的产率产生L-谷氨酰胺；本发明还涉及用该微生物产生L-谷氨酰胺的方法。
                         背景技术
L-谷氨酰胺是一种广泛用作药物，例如肠胃疾病的治疗剂，肝脏和脑功能的增强剂，免疫增强剂，以及胃溃疡和酒精中毒的治疗剂等，化妆品如增湿剂等，以及健康食品如运动营养物和病人的营养物等的氨基酸。
根据现有技术，L-谷氨酰胺获自磺胺胍抗性菌株(日本专利延迟公开号Sho53-17675)中，重氮丝氨酸抗性菌株(日本专利延迟公开号Sho55-148094)，氨苄青霉素敏感菌株(日本专利延迟公开号Hei04-088994)，酪氨酸-谷氨酸(tyr-glu)抗性菌株(日本专利延迟公开号Hei02-186994)等。
                         发明公开
本发明人进行了广泛的研究以发展新型的能够高产地产生L-谷氨酰胺的菌株。我们预期抗叠氮钠(一种呼吸抑制剂)或者抗α-氨基丁酸(异亮氨酸的类似物)的菌株将具有增加的L-谷氨酰胺生产能力。因此我们从原始菌株乳发酵短杆菌KFCC-10680(韩国专利公开文本91-7818)中筛选叠氮钠或者α-氨基丁酸抗性的菌株。结果，我们鉴定出叠氮钠或者α-氨基丁酸抗性菌株要比已知菌株具有更高的L-谷氨酰胺产率，因而完成本发明。
本发明提供产生L-谷氨酰胺的微生物和使用该微生物产生L-谷氨酰胺的方法。根据本发明的微生物为具有叠氮钠抗性的乳发酵短杆菌CJJA21(KCCM-10222)和具有α-氨基丁酸抗性的乳发酵短杆菌CJJA22(KCCM-10223)，这两者均产生高产量的L-谷氨酰胺。此外，本发明产生L-谷氨酰胺的方法的特征在于活化乳发酵短杆菌CJJA21或者乳发酵短杆菌CJJA22，然后培养该活化菌株。
本发明通过以下方法诱导突变体。乳发酵短杆菌KFCC-10680用传统诱变剂N-甲基-N’-硝基-N-亚硝基胍(NTG)处理，然后铺于含有500mg/l叠氮钠的基本培养基(培养基1)中，由此获得具有500mg/l叠氮钠抗性的菌株。
更加具体的说，乳发酵短杆菌KFCC-10680，先在活化培养基(培养基2)中培养16小时活化，再在已121℃灭菌15分钟的种子培养基(培养基3)中培养14个小时。然后，将5ml的培养基用100mM的柠檬酸盐缓冲液洗涤，加入NTG至最终浓度为200mg/l。在20分钟之后，培养基用100mM的磷酸盐缓冲液洗涤。用NTG处理的菌株铺在基本培养基(培养基1)中并测量死亡率。结果，死亡率为85％。
为了获得叠氮钠抗性的突变体，将NTG处理的菌株铺在含有终浓度为500mg/l的叠氮钠的基本培养基(培养基1)中，然后在30℃培养6天获得叠氮钠抗性的菌株。获得的抗性突变体在含有谷氨酰胺生产培养基(培养基4)的摇瓶中培养72个小时，选择产生L-谷氨酰胺的产率比原始菌株乳发酵短杆菌KFCC-10680高10％甚至更高的叠氮钠抗性菌株。获得的菌株命名为CJJA21。乳发酵短杆菌CJJA21按照布达佩斯条约于2000年10月20日保藏在韩国微生物保藏中心(Korean Culture Center ofMicroorganisms)，地址为Hongie-dong，Seodaemun-gu，Seoul，保藏号为KCCM-10222。
此外，乳发酵短杆菌KFCC-10680基本上按上述方式活化和培养。随后基本上按上述方式用NTG处理。为了获得α-氨基丁酸抗性突变体，NTG处理的菌株铺在α-氨基丁酸终浓度为15g/l的基本培养基(培养基1)上，在30℃培养6天获得α-氨基丁酸抗性菌株。获得的抗性突变体在含有谷氨酰胺生产培养基(培养基4)的摇瓶中培养72小时，选择产生L-谷氨酰胺的产率比原始菌株乳发酵短杆菌KFCC-10680高10％甚至更高的叠氮钠抗性菌株。获得的菌株命名为CJJA22。乳发酵短杆菌CJJA22按照布达佩斯条约于2000年10月20日保藏在韩国微生物保藏中心，地址为Hongie-dong，Seodaemun-gu，Seoul，保藏号为KCCM-10223。
本发明使用的培养基含有以下成分：
培养基1：基本培养基
葡萄糖1.0％，硫酸铵((NH4)2SO4)0.4％，硫酸镁(MgSO4.7H2O)0.04％，磷酸二氢钾(KH2PO4)0.1％，尿素0.1％，硫胺素.HCl 0.0001％，生物素200μg/l，琼脂，pH7.0。
培养基2：活化培养基
牛肉膏1％，聚胨1％，氯化钠(NaCl)0.5％，酵母提取物0.5％，琼脂2％，琼脂2％，pH7.2
培养基3：种子培养基
葡萄糖5.0％，Bactopeptone 1％，氯化钠(NaCl)0.25％，酵母提取物1％，生物素3μg/l，尿素0.4％，pH7.0
培养基4：谷氨酰胺生产培养基
葡萄糖4.0％，氯化铵(NH4Cl)3.0％，豆蛋白酸水解产物0.3％，碳酸钙(CaCO3)5％，氯化钙(CaCl2)0.1％，硫酸镁(MgSO4·7H2O)0.05％，磷酸二氢钾(KH2PO4)0.15％，磷酸氢二钾(K2HPO4)0.15％，尿素0.3％，硫胺素·HCl 2mg/l，生物素5ug/l，硫酸亚铁(FeSO4·7H2O)20mg/L，硫酸锰(MnSO4·H2O)20mg/L，硫酸锌(ZnSO4·7H2O)12mg/l，pH6.8。
乳发酵短杆菌CJJA21的叠氮钠抗性见下表1-1所示。
                             表1-1
 菌株                 叠氮钠浓度(mg/l)
  0     100     200     300     500     800
 KFCC-10680   +++     +     +     -     -     -
 CJJA21   +++     +++     +++     +++     ++     -
+：生长，-：不生长，在30℃培养6天
乳发酵短杆菌CJJA22的α-氨基丁酸抗性见下表1-2所示。
                            表1-2
菌株                 α-氨基丁酸浓度(g/l)
  0   1     5   10   15     20
KFCC-10680   +++   ++     +   -   -     -
CJJA22   +++   +++   +++   +++   ++     -
+：生长，-：不生长，在30℃培养6天
                   实施本发明的最好方式
从以下实施例可以得到更好地理解本发明。然而，本领域普通技术人员能够理解，这里描述的具体材料和结果仅仅是举例说明、而不是企图限制、也不应限制在权利要求书中详细说明的本发明。
                         实施例1
菌株：
    乳发酵短杆菌CJJA21
发酵培养基：
葡萄糖4.0％，氯化铵(NH4Cl)3.0％，豆蛋白酸水解产物0.3％，碳酸钙(CaCO3)5％，氯化钙(CaCl2)0.1％，硫酸镁(MgSO4·7H2O)0.05％，磷酸二氢钾(KH2PO4)0.15％，磷酸氢二钾(K2HPO4)0.15％，尿素0.3％，硫胺素·HCl 2mg/l，生物素5ug/l，硫酸亚铁(FeSO4·7H2O)20mg/L，硫酸锰(MnSO4·H2O)20mg/L，硫酸锌(ZnSO4·7H2O)12mg/l，pH6.8(和培养基4相同)
发酵步骤和结果：
向250ml的摇瓶中加入20ml发酵培养基。培养基在121℃灭菌15分钟。接种一个接种环的通过在活化培养基(培养基2)中30℃培养16小时活化的菌株，然后在30℃摇菌48小时。发酵肉汤的成分如下。
                            表2
 KFCC-10680(原始菌株)     CJJA(突变体)
谷氨酰胺浓度(g/l)  12.6     14.5
                         实施例2
菌株：
    乳发酵短杆菌CJJA21
发酵培养基：
葡萄糖10％，氯化铵(NH4Cl)4.5％，豆蛋白酸水解产物0.5％，碳酸钙(CaCO3)5％，氯化钙(CaCl2)0.1％，硫酸镁(MgSO4·7H2O)0.05％，磷酸二氢钾(KH2PO4)0.15％，磷酸氢二钾(K2HPO4)0.15％，尿素0.3％，硫胺素·HCl 2mg/l，生物素5ug/l，硫酸亚铁(FeSO4·7H2O)20mg/L，硫酸锰(MnSO4·H2O)20mg/L，硫酸锌(ZnSO4·7H2O)12mg/l，pH6.8
发酵步骤和结果：
向250ml的摇瓶加入20ml发酵培养基。培养基在121℃灭菌15分钟。接种一个接种环的通过在活化培养基(培养基2)中30℃培养16小时活化的菌株，然后在30℃摇菌72小时。发酵肉汤的成分如下。
                            表3
   KFCC-10680(原始菌株)     CJJA21(突变体)
谷氨酰胺浓度(g/l)    31.5     37.1
如表3所示，本发明乳发酵短杆菌CJJA21产生L-谷氨酰胺的产率要比原始菌株乳发酵短杆菌KFCC-10680的高出10％或者更高。
                         实施例3
乳发酵短杆菌CJJA22根据基本上与实施例1相同的方法进行培养。发酵肉汤的成分如下：
                                 表4
    KFCC-10680(原始菌株)     CJJA(突变体)
    谷氨酰胺浓度(g/l)     12.4     14.1
                         实施例4
乳发酵短杆菌CJJA22根据基本上与实施例2相同的方法进行培养。发酵肉汤的成分如下：
                             表5
    KFCC-10680(原始菌株)     CJJA(突变体)
    谷氨酰胺浓度(g/l)     31.1     36.5
                         工业应用
根据本发明，L-谷氨酰胺可以以高于现有技术的产率获得。获得的L-谷氨酰胺可用作药物，例如肠胃疾病的治疗剂，肝脏和脑功能的增强剂，免疫增强剂，胃溃疡和酒精中毒的治疗剂等；化妆品如增湿剂等；和健康食品如运动营养物和病人的营养物等。

Claims (3)

1.乳发酵短杆菌CJJA21，其特征在于具有叠氮钠抗性并产生L-谷氨酰胺(保藏号KCCM-10222)。
2.乳发酵短杆菌CJJA22，其特征在于具有α-氨基丁酸抗性并产生L-谷氨酰胺(保藏号KCCM-10223)。
3.产生L-谷氨酰胺的方法，其特征在于活化权利要求1的乳发酵短杆菌CJJA21或者权利要求2的乳发酵短杆菌CJJA22，然后培养该活化菌株。
CNB01803831XA 2000-11-17 2001-11-15 产生l-谷氨酰胺的微生物和使用该微生物产生l-谷氨酰胺的方法 Expired - Fee Related CN1181190C (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR10-2000-0068284A KR100397318B1 (ko) 2000-11-17 2000-11-17 엘-글루타민을 생산하는 브레비박테리움 락토퍼멘텀씨제이제이에이21 및 그를 이용한 엘-글루타민 생산방법
KR10-2000-0068285A KR100397317B1 (ko) 2000-11-17 2000-11-17 엘-글루타민을 생산하는 브레비박테리움 락토퍼멘텀씨제이제이에이22 및 그를 이용한 엘-글루타민 생산방법
KR2000/68285 2000-11-17
KR2000/68284 2000-11-17

Publications (2)

Publication Number Publication Date
CN1395615A true CN1395615A (zh) 2003-02-05
CN1181190C CN1181190C (zh) 2004-12-22

Family

ID=26638544

Family Applications (1)

Application Number Title Priority Date Filing Date
CNB01803831XA Expired - Fee Related CN1181190C (zh) 2000-11-17 2001-11-15 产生l-谷氨酰胺的微生物和使用该微生物产生l-谷氨酰胺的方法

Country Status (9)

Country Link
US (2) US6984506B2 (zh)
EP (1) EP1334176B1 (zh)
JP (2) JP2004513654A (zh)
CN (1) CN1181190C (zh)
AT (1) ATE389710T1 (zh)
AU (1) AU783498B2 (zh)
DE (1) DE60133304T2 (zh)
DK (1) DK1334176T3 (zh)
WO (1) WO2002040643A1 (zh)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104830923A (zh) * 2015-04-29 2015-08-12 宁夏诚志万胜生物工程有限公司 一种利用嗜热乳杆菌从l-谷氨酸生物转化法生产l-谷氨酰胺的方法

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002040643A1 (en) * 2000-11-17 2002-05-23 Cheil Jedang Corporation Microorganisms producing l-glutamine and processes for producing l-glutamine using the same
JP2007143938A (ja) * 2005-11-29 2007-06-14 Strong Co Ltd スリッパ
KR100791659B1 (ko) * 2006-07-13 2008-01-03 씨제이 주식회사 알지닌을 생산하는 미생물 및 이를 이용한 엘-알지닌의제조방법
KR101694850B1 (ko) * 2014-10-08 2017-01-10 씨제이제일제당 주식회사 L-글루타민을 생산하는 미생물 및 이를 이용한 l-글루타민 생산방법

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5317675B2 (zh) * 1972-12-16 1978-06-09
JPS5317675A (en) 1976-08-03 1978-02-17 Tsunetarou Matsuyama Method of processing disposed polyvinyl chloride
JPS5462388A (en) 1977-10-26 1979-05-19 Ajinomoto Co Inc Preparation of l-glutamin by fermentation
JPS55148094A (en) 1979-05-07 1980-11-18 Kyowa Hakko Kogyo Co Ltd Preparation of l-glutamine by fermentation
JPS5810075B2 (ja) * 1979-08-31 1983-02-24 味の素株式会社 新規変異株
JPS56164792A (en) * 1980-05-24 1981-12-17 Ajinomoto Co Inc Production of l-glutamine through fermentation process
US4411991A (en) * 1980-10-07 1983-10-25 Kanegafuchi Chemical Industry Company, Limited Process for fermentative production of amino acids
JPS60248195A (ja) * 1984-05-22 1985-12-07 Ajinomoto Co Inc 発酵法によるｌ−グルタミンの製造法
JPS61202694A (ja) * 1985-03-07 1986-09-08 Ajinomoto Co Inc 発酵法によるｌ−グルタミンの製造法
US5164307A (en) * 1987-01-23 1992-11-17 Ajinomoto Co., Inc. Process for producing L-amino acids by fermentation
JP2817157B2 (ja) 1989-01-13 1998-10-27 味の素株式会社 発酵法によるｌ‐アミノ酸の製造法
KR910007818B1 (ko) * 1989-10-21 1991-10-02 제일제당 주식회사 글루타민(Glutamine)을 생산하는 미생물
JPH0488994A (ja) 1990-07-30 1992-03-23 Kyowa Hakko Kogyo Co Ltd 発酵法によるｌ―グルタミン酸の製造法
DE69116964T2 (de) * 1990-09-18 1996-10-02 Kyowa Hakko Kogyo Kk Verfahren zur Herstellung von L-Valin
BR9917880B1 (pt) * 1998-09-25 2014-02-18 Processo para produzir um ácido l-glutâmico
JP4304837B2 (ja) * 2000-07-05 2009-07-29 味の素株式会社 Ｌ−グルタミン酸生産菌及びｌ−グルタミン酸の製造法
WO2002040643A1 (en) * 2000-11-17 2002-05-23 Cheil Jedang Corporation Microorganisms producing l-glutamine and processes for producing l-glutamine using the same
EP1424397B1 (en) * 2002-11-26 2011-01-19 Ajinomoto Co., Inc. Method for producing L-glutamine and L-glutamine producing bacterium
US20050186973A1 (en) 2004-02-04 2005-08-25 Peter Gaal Method and apparatus for sending signaling for multicast and broadcast services

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104830923A (zh) * 2015-04-29 2015-08-12 宁夏诚志万胜生物工程有限公司 一种利用嗜热乳杆菌从l-谷氨酸生物转化法生产l-谷氨酰胺的方法
CN104830923B (zh) * 2015-04-29 2018-05-18 宁夏诚志万胜生物工程有限公司 一种利用嗜热乳杆菌从l-谷氨酸生物转化法生产l-谷氨酰胺的方法

Also Published As

Publication number Publication date
DE60133304D1 (de) 2008-04-30
AU2313902A (en) 2002-05-27
US20050250187A1 (en) 2005-11-10
CN1181190C (zh) 2004-12-22
WO2002040643A1 (en) 2002-05-23
JP2004513654A (ja) 2004-05-13
EP1334176A1 (en) 2003-08-13
US7192760B2 (en) 2007-03-20
ATE389710T1 (de) 2008-04-15
DE60133304T2 (de) 2009-03-05
US6984506B2 (en) 2006-01-10
AU783498B2 (en) 2005-11-03
DK1334176T3 (da) 2008-07-14
US20030096380A1 (en) 2003-05-22
EP1334176B1 (en) 2008-03-19
EP1334176A4 (en) 2006-04-05
JP2005323608A (ja) 2005-11-24

Similar Documents

Publication Publication Date Title
RU2209248C2 (ru) Способ получения l-метионина, штамм бактерии escherichia coli вкпм в-8125 - продуцент l-метионина
US7244608B2 (en) Microorganism producing 5′-inosinic acid and process for producing 5′-inosinic acid using the same
CN1181190C (zh) 产生l-谷氨酰胺的微生物和使用该微生物产生l-谷氨酰胺的方法
CN1249221C (zh) 过度生产5’－黄苷酸的微生物
CN1140761A (zh) L-亮氨酸的生产方法
SU1719433A1 (ru) Способ получени L-аланина
CN1022931C (zh) 一种生产l-赖氨酸的方法
JP2578463B2 (ja) 発酵法によるｌ−リジンの製造法
Lee et al. Suitable conditions for mycelial culture of Tremella fuciformis.
WO1998048033A1 (en) STRAIN PRODUCING REMARKABLE AMOUNT OF ε-POLY-L-LYSINE AND PROCESS FOR PRODUCING ε-POLY-L-LYSINE BY USING THE SAME
KR100397317B1 (ko) 엘-글루타민을 생산하는 브레비박테리움 락토퍼멘텀씨제이제이에이22 및 그를 이용한 엘-글루타민 생산방법
KR100397318B1 (ko) 엘-글루타민을 생산하는 브레비박테리움 락토퍼멘텀씨제이제이에이21 및 그를 이용한 엘-글루타민 생산방법
KR890000538B1 (ko) 미생물에 의한 5'-이노신산의 제조방법
KR830001259B1 (ko) 미생물에 의한 l-글루타민의 제조방법
KR820000523B1 (ko) 미생물에 의한 l-알기닌의 제조법
EP0117741B1 (en) Process for producing l-histidine by fermentation and microorganisms for use therein
CN1065538C (zh) 抗生素斯泰隆巴森
KR800000476B1 (ko) 미생물에 의한 아데노신의 제조법
KR830001258B1 (ko) 미생물에 의한 l-글루타민의 제조방법
KR970002250B1 (ko) 5-크산틸산(5'-Xanthyl산)을 생성하는 미생물
KR20020057472A (ko) 5'-크산틸산을 고수율로 생산하는 미생물 코리네박테리움암모니아게네스 씨제이엑스피002 및 그를 이용한5'-크산틸산 생산방법
KR19990049691A (ko) 코리네박테리움 글루타미컴 cj88 및 이 균주를 이용한l-라이신의 제조방법
JPH0425799B2 (zh)
JPH0211238B2 (zh)
JPS6066990A (ja) Ｌ−グルタミン酸の製造法

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
ASS Succession or assignment of patent right

Owner name: CJ CHEIL JEDANG CORP.

Free format text: FORMER OWNER: CJ CORP.

Effective date: 20080328

C41 Transfer of patent application or patent right or utility model
C56 Change in the name or address of the patentee

Owner name: CJ CORP.

Free format text: FORMER NAME OR ADDRESS: CHEIL JEDANG CORP.

CP03 Change of name, title or address

Address after: Seoul, South Kerean

Patentee after: CJ Corp.

Address before: Seoul

Patentee before: CHEIL JEDANG Corp.

TR01 Transfer of patent right

Effective date of registration: 20080328

Address after: Seoul, South Kerean

Patentee after: CJ CHEILJEDANG Corp.

Address before: Seoul, South Kerean

Patentee before: CJ Corp.

C17 Cessation of patent right
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20041222

Termination date: 20091215