CN114901701A - 一种超低分子量透明质酸及其制备方法 - Google Patents
一种超低分子量透明质酸及其制备方法 Download PDFInfo
- Publication number
- CN114901701A CN114901701A CN202080090006.6A CN202080090006A CN114901701A CN 114901701 A CN114901701 A CN 114901701A CN 202080090006 A CN202080090006 A CN 202080090006A CN 114901701 A CN114901701 A CN 114901701A
- Authority
- CN
- China
- Prior art keywords
- hyaluronic acid
- molecular weight
- content
- low molecular
- ultra
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 229920002674 hyaluronan Polymers 0.000 title claims abstract description 198
- 229960003160 hyaluronic acid Drugs 0.000 title claims abstract description 198
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 title claims abstract description 166
- 238000002360 preparation method Methods 0.000 title abstract description 6
- -1 hyaluronic acid disaccharide Chemical class 0.000 claims abstract description 32
- 238000000034 method Methods 0.000 claims abstract description 30
- 239000000203 mixture Substances 0.000 claims abstract description 29
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 17
- 108010003272 Hyaluronate lyase Proteins 0.000 claims abstract description 17
- 102000001974 Hyaluronidases Human genes 0.000 claims abstract description 17
- 229960002773 hyaluronidase Drugs 0.000 claims abstract description 17
- KIUKXJAPPMFGSW-XZLGHBIVSA-N beta-D-GlcpA-(1->3)-beta-D-GlcpNAc-(1->4)-beta-D-GlcpA-(1->3)-D-GlcpNAc Chemical compound CC(=O)N[C@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-XZLGHBIVSA-N 0.000 claims abstract description 11
- 238000001694 spray drying Methods 0.000 claims abstract description 7
- 238000010438 heat treatment Methods 0.000 claims abstract description 6
- 239000002994 raw material Substances 0.000 claims abstract description 6
- 238000001914 filtration Methods 0.000 claims abstract description 5
- 238000004519 manufacturing process Methods 0.000 claims abstract description 4
- 230000002779 inactivation Effects 0.000 claims abstract description 3
- 238000009826 distribution Methods 0.000 claims description 15
- 238000003756 stirring Methods 0.000 claims description 14
- 150000002016 disaccharides Chemical class 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 230000008439 repair process Effects 0.000 claims description 11
- 238000006243 chemical reaction Methods 0.000 claims description 10
- 230000001737 promoting effect Effects 0.000 claims description 9
- 230000008569 process Effects 0.000 claims description 8
- 239000002537 cosmetic Substances 0.000 claims description 7
- 239000008213 purified water Substances 0.000 claims description 7
- 238000001179 sorption measurement Methods 0.000 claims description 6
- 150000004044 tetrasaccharides Chemical class 0.000 claims description 6
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 4
- 229940079593 drug Drugs 0.000 claims description 4
- 239000003814 drug Substances 0.000 claims description 4
- 229910052739 hydrogen Inorganic materials 0.000 claims description 4
- 229910052700 potassium Inorganic materials 0.000 claims description 4
- 231100000245 skin permeability Toxicity 0.000 claims description 4
- 229910052708 sodium Inorganic materials 0.000 claims description 4
- 241000545744 Hirudinea Species 0.000 claims description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 3
- 230000036541 health Effects 0.000 claims description 3
- 239000002775 capsule Substances 0.000 claims description 2
- 239000007810 chemical reaction solvent Substances 0.000 claims description 2
- 230000007071 enzymatic hydrolysis Effects 0.000 claims description 2
- 239000011541 reaction mixture Substances 0.000 claims description 2
- SNRUBQQJIBEYMU-UHFFFAOYSA-N Dodecane Natural products CCCCCCCCCCCC SNRUBQQJIBEYMU-UHFFFAOYSA-N 0.000 claims 1
- 150000001720 carbohydrates Chemical class 0.000 claims 1
- 125000003438 dodecyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 claims 1
- 230000001954 sterilising effect Effects 0.000 claims 1
- 230000007062 hydrolysis Effects 0.000 abstract description 4
- 238000006460 hydrolysis reaction Methods 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 3
- 238000010364 biochemical engineering Methods 0.000 abstract description 2
- 239000003960 organic solvent Substances 0.000 abstract description 2
- 238000009776 industrial production Methods 0.000 abstract 1
- 229920001542 oligosaccharide Polymers 0.000 description 24
- 239000000047 product Substances 0.000 description 14
- 230000000694 effects Effects 0.000 description 13
- 239000012488 sample solution Substances 0.000 description 12
- 239000000243 solution Substances 0.000 description 12
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 10
- 102000004190 Enzymes Human genes 0.000 description 8
- 108090000790 Enzymes Proteins 0.000 description 8
- 229940088598 enzyme Drugs 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 7
- 230000004044 response Effects 0.000 description 7
- 150000002482 oligosaccharides Chemical class 0.000 description 5
- 239000011521 glass Substances 0.000 description 4
- 230000003020 moisturizing effect Effects 0.000 description 4
- 210000003491 skin Anatomy 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 241000700159 Rattus Species 0.000 description 3
- DQPBABKTKYNPMH-UHFFFAOYSA-N amino hydrogen sulfate Chemical compound NOS(O)(=O)=O DQPBABKTKYNPMH-UHFFFAOYSA-N 0.000 description 3
- 239000000706 filtrate Substances 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 239000000413 hydrolysate Substances 0.000 description 3
- 238000000053 physical method Methods 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- 229920002385 Sodium hyaluronate Polymers 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 210000000988 bone and bone Anatomy 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 230000000593 degrading effect Effects 0.000 description 2
- 210000002615 epidermis Anatomy 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 229920002521 macromolecule Polymers 0.000 description 2
- 230000003204 osmotic effect Effects 0.000 description 2
- 239000007800 oxidant agent Substances 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 229940010747 sodium hyaluronate Drugs 0.000 description 2
- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 230000001502 supplementing effect Effects 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 1
- 102400000888 Cholecystokinin-8 Human genes 0.000 description 1
- 101800005151 Cholecystokinin-8 Proteins 0.000 description 1
- IAJILQKETJEXLJ-UHFFFAOYSA-N Galacturonsaeure Natural products O=CC(O)C(O)C(O)C(O)C(O)=O IAJILQKETJEXLJ-UHFFFAOYSA-N 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 241000132152 Polymyxa Species 0.000 description 1
- 206010060932 Postoperative adhesion Diseases 0.000 description 1
- 241000235342 Saccharomycetes Species 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 238000005903 acid hydrolysis reaction Methods 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 238000005904 alkaline hydrolysis reaction Methods 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000033115 angiogenesis Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 230000003833 cell viability Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 210000004207 dermis Anatomy 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 210000001339 epidermal cell Anatomy 0.000 description 1
- 238000002270 exclusion chromatography Methods 0.000 description 1
- 239000003889 eye drop Substances 0.000 description 1
- 229940012356 eye drops Drugs 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 230000036571 hydration Effects 0.000 description 1
- 238000006703 hydration reaction Methods 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 238000010166 immunofluorescence Methods 0.000 description 1
- 230000005847 immunogenicity Effects 0.000 description 1
- 238000003364 immunohistochemistry Methods 0.000 description 1
- 230000007365 immunoregulation Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 230000001050 lubricating effect Effects 0.000 description 1
- 238000005461 lubrication Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 238000010525 oxidative degradation reaction Methods 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000011027 product recovery Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- IZTQOLKUZKXIRV-YRVFCXMDSA-N sincalide Chemical compound C([C@@H](C(=O)N[C@@H](CCSC)C(=O)NCC(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC=1C=CC=CC=1)C(N)=O)NC(=O)[C@@H](N)CC(O)=O)C1=CC=C(OS(O)(=O)=O)C=C1 IZTQOLKUZKXIRV-YRVFCXMDSA-N 0.000 description 1
- 231100000075 skin burn Toxicity 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 230000009469 supplementation Effects 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 210000004127 vitreous body Anatomy 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
- C08B37/0072—Hyaluronic acid, i.e. HA or hyaluronan; Derivatives thereof, e.g. crosslinked hyaluronic acid (hylan) or hyaluronates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/12—Disaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/10—General cosmetic use
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Materials Engineering (AREA)
- Polymers & Plastics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Chemical & Material Sciences (AREA)
- Dermatology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Sustainable Development (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Cosmetics (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
本发明公开了一种超低分子量透明质酸及其制备方法，属于生物化工技术领域。本发明利用大分子透明质酸为原料，经过透明质酸酶水解、加热灭活、活性炭过滤及喷雾干燥等生产工艺，可得到平均分子量低于1200道尔顿的超低分子量透明质酸产物。其为透明质酸二糖至十二糖的混合物，透明质酸二糖含量占比为5‑40％，透明质酸四糖含量占比40‑70％，透明质酸六糖含量占比10‑30％，透明质酸八糖含量占比1‑15％，透明质酸十糖含量占比1‑10％，透明质酸十糖以上含量占比低于6％，与普通市售的低分子透明质酸相比，该产品拥有更显著的渗透补水与促进修复能力，可广泛用于医药产品、保健产品、化妆品等领域。该方法操作简便、条件温和、未使用有机溶剂、酶解效率高，适宜大规模工业化生产。
Description
PCT国内申请，说明书已公开。
Claims (11)
- PCT国内申请，权利要求书已公开。
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911331251.7A CN111040048A (zh) | 2019-12-21 | 2019-12-21 | 一种超低分子量透明质酸及其制备方法 |
| CN2019113312517 | 2019-12-21 | ||
| PCT/CN2020/091956 WO2021120521A1 (zh) | 2019-12-21 | 2020-05-23 | 一种超低分子量透明质酸及其制备方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114901701A true CN114901701A (zh) | 2022-08-12 |
Family
ID=70238301
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911331251.7A Pending CN111040048A (zh) | 2019-12-21 | 2019-12-21 | 一种超低分子量透明质酸及其制备方法 |
| CN202080090006.6A Pending CN114901701A (zh) | 2019-12-21 | 2020-05-23 | 一种超低分子量透明质酸及其制备方法 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911331251.7A Pending CN111040048A (zh) | 2019-12-21 | 2019-12-21 | 一种超低分子量透明质酸及其制备方法 |
Country Status (3)
| Country | Link |
|---|---|
| US (1) | US20220380488A1 (zh) |
| CN (2) | CN111040048A (zh) |
| WO (1) | WO2021120521A1 (zh) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111040048A (zh) * | 2019-12-21 | 2020-04-21 | 南京汉欣医药科技有限公司 | 一种超低分子量透明质酸及其制备方法 |
| CN111893151A (zh) * | 2020-08-21 | 2020-11-06 | 浙江绿创生物科技股份有限公司 | 一种连续化生产低分子量透明质酸的方法及所得低分子量透明质酸和应用 |
| CN115029402A (zh) * | 2021-03-05 | 2022-09-09 | 湖州蔻婷生物科技有限公司 | 一种寡聚透明质酸及其水解胶原蛋白和纤连蛋白复配化妆品组合物 |
| CN113150187B (zh) * | 2021-05-28 | 2022-03-25 | 杨彦威 | 一种超低分子量质酸钙与脲的络合物及其制备方法与应用 |
| CN113493776B (zh) * | 2021-07-07 | 2022-06-14 | 山东焦点福瑞达生物股份有限公司 | 一种连续制备酶切超低分子量透明质酸或其盐的方法 |
| CN113801904A (zh) * | 2021-09-17 | 2021-12-17 | 山东华熙海御生物医药有限公司 | 一种透明质酸寡糖组合物及其制备方法和用途 |
| CN113876623B (zh) * | 2021-09-24 | 2024-06-14 | 华熙生物科技股份有限公司 | 一种透明质酸寡糖组合物在抗皮肤衰老、促进胶原蛋白生成中的用途 |
| CN113717236B (zh) * | 2021-09-24 | 2023-06-02 | 常熟纳微生物科技有限公司 | 一种透明质酸的分离纯化方法 |
| CN114288308B (zh) * | 2021-12-02 | 2024-08-30 | 华熙生物科技股份有限公司 | 一种以四糖为主的透明质酸寡糖组合物及其制备方法和应用 |
| CN114133419B (zh) * | 2021-12-21 | 2022-11-15 | 南京乐韬生物科技有限公司 | 一种低分子量乙酰化透明质酸盐及其制备方法和应用 |
| CN114574532B (zh) * | 2022-03-29 | 2024-05-14 | 水羊化妆品制造有限公司 | 一种透明质酸二四六糖的制备方法 |
| CN116179629A (zh) * | 2023-03-06 | 2023-05-30 | 水羊化妆品制造有限公司 | 不饱和透明质酸钠二糖的制备方法 |
| CN117143939B (zh) * | 2023-07-12 | 2024-08-13 | 菏泽市亿鑫生物科技有限公司 | 低分子透明质酸发酵液、含其产品及其制备方法和应用 |
| CN116694705B (zh) * | 2023-08-03 | 2023-10-20 | 北商加美(北京)科技有限公司 | 超低分子透明质酸发酵液、含其产品、及其制备和应用 |
| CN117535363A (zh) * | 2023-12-27 | 2024-02-09 | 常州加源医药科技研发有限公司 | 一种功能性透明质酸的生产方法 |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101507733A (zh) * | 2009-04-02 | 2009-08-19 | 北京中生奥普寡肽技术研究所 | 纳米小分子透明质酸及其制备方法 |
| CN103484513A (zh) * | 2013-10-22 | 2014-01-01 | 江南大学 | 一种酶法制备小分子寡聚透明质酸的方法 |
| CN104120158A (zh) * | 2014-07-01 | 2014-10-29 | 江南大学 | 一种添加透明质酸酶提高小分子透明质酸发酵产量的方法 |
| CN104178539A (zh) * | 2014-08-19 | 2014-12-03 | 江南大学 | 一种规模化制备特定分子量小分子透明质酸的方法 |
| US20150031085A1 (en) * | 2013-07-29 | 2015-01-29 | Jiangnan University | Novel Leech Hyaluronidase and Its Application |
| CN104610467A (zh) * | 2015-01-27 | 2015-05-13 | 江南大学 | 一种透明质酸四糖和六糖的分离方法 |
| CN106399428A (zh) * | 2016-09-29 | 2017-02-15 | 江南大学 | 一种高效分离制备单一分子量透明质酸寡糖的方法 |
| CN109097421A (zh) * | 2018-08-14 | 2018-12-28 | 江南大学 | 一种双酶水解制备透明质酸奇数寡糖的方法 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1171375A (en) * | 1980-09-15 | 1984-07-24 | Ulf P.F. Lindahl | Oligosaccharides having selective anticoagulation activity |
| KR101132114B1 (ko) * | 2009-09-15 | 2012-04-05 | 일동제약주식회사 | 히알루론산의 분자량을 조절하는 방법 |
| CN102304193A (zh) * | 2011-09-29 | 2012-01-04 | 胡如桂 | 一种寡聚透明质酸的制备方法及其用途 |
| KR102013603B1 (ko) * | 2018-12-20 | 2019-08-23 | (주)성우제네텍 | 감마선 조사 및 히알루론산분해효소 처리에 의한 히알루론산의 저분자화 방법 |
| CN111040048A (zh) * | 2019-12-21 | 2020-04-21 | 南京汉欣医药科技有限公司 | 一种超低分子量透明质酸及其制备方法 |
-
2019
- 2019-12-21 CN CN201911331251.7A patent/CN111040048A/zh active Pending
-
2020
- 2020-05-23 CN CN202080090006.6A patent/CN114901701A/zh active Pending
- 2020-05-23 WO PCT/CN2020/091956 patent/WO2021120521A1/zh active Application Filing
-
2022
- 2022-06-21 US US17/845,248 patent/US20220380488A1/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101507733A (zh) * | 2009-04-02 | 2009-08-19 | 北京中生奥普寡肽技术研究所 | 纳米小分子透明质酸及其制备方法 |
| US20150031085A1 (en) * | 2013-07-29 | 2015-01-29 | Jiangnan University | Novel Leech Hyaluronidase and Its Application |
| CN103484513A (zh) * | 2013-10-22 | 2014-01-01 | 江南大学 | 一种酶法制备小分子寡聚透明质酸的方法 |
| CN104120158A (zh) * | 2014-07-01 | 2014-10-29 | 江南大学 | 一种添加透明质酸酶提高小分子透明质酸发酵产量的方法 |
| CN104178539A (zh) * | 2014-08-19 | 2014-12-03 | 江南大学 | 一种规模化制备特定分子量小分子透明质酸的方法 |
| CN104610467A (zh) * | 2015-01-27 | 2015-05-13 | 江南大学 | 一种透明质酸四糖和六糖的分离方法 |
| CN106399428A (zh) * | 2016-09-29 | 2017-02-15 | 江南大学 | 一种高效分离制备单一分子量透明质酸寡糖的方法 |
| CN109097421A (zh) * | 2018-08-14 | 2018-12-28 | 江南大学 | 一种双酶水解制备透明质酸奇数寡糖的方法 |
Non-Patent Citations (1)
| Title |
|---|
| 吕梦娴: "《透明质酸四糖和六糖的制备及生物活性研究》", 《中国优秀博硕士学位论文全文数据库(硕士)工程科技Ⅰ辑》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2021120521A1 (zh) | 2021-06-24 |
| US20220380488A1 (en) | 2022-12-01 |
| CN111040048A (zh) | 2020-04-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN114901701A (zh) | 一种超低分子量透明质酸及其制备方法 | |
| CN108220364B (zh) | 一种固液双相酶解与超滤联用制备超低分子量透明质酸寡糖及其盐的方法 | |
| CN113512134B (zh) | 一种全分子量分布的透明质酸钠及其制备方法和应用 | |
| JP7105888B2 (ja) | 低分子ヒアルロン酸又はその塩及びその調製方法 | |
| EP2236523B1 (en) | Method for producing cross-linked hyaluronic acid | |
| CN110331178B (zh) | 一种酶切法制备小分子透明质酸的方法及所得小分子透明质酸与其应用 | |
| CN106387923B (zh) | 一种富含半乳甘露聚糖的可溶性膳食纤维及其制备方法 | |
| KR20100094982A (ko) | 점탄성 바이오폴리머의 희석식 여과 멸균 방법 | |
| JP5732390B2 (ja) | 酵母ピキア・パストリスの発酵によるキチン、その誘導体及びグルコース、マンノース及び／又はガラクトースを含有するポリマーの共生産のための方法 | |
| CN116003577B (zh) | 一种用于皮肤舒缓修复的重组胶原蛋白及其应用 | |
| WO2021081999A1 (zh) | 一种低分子量硫酸软骨素及其制备方法 | |
| Iqbal et al. | Enzymatic and acidic degradation of high molecular weight dextran into low molecular weight and its characterizations using novel Diffusion-ordered NMR spectroscopy | |
| JP5340093B2 (ja) | 架橋ヒアルロン酸の製造方法 | |
| KR101132114B1 (ko) | 히알루론산의 분자량을 조절하는 방법 | |
| CN114288308A (zh) | 一种以四糖为主的透明质酸寡糖组合物及其制备方法和应用 | |
| JP2020507666A (ja) | オゾンを用いた多糖類の分解方法 | |
| CN101168570B (zh) | 海带硫酸多糖的降解方法 | |
| CN116196262A (zh) | 一种酵母细胞壁提取物水解物的制备方法及化妆品组合物 | |
| CN115944549A (zh) | 透明质酸寡糖组合物及其制备方法和用途 | |
| CN113827763A (zh) | 一种中药成分改性的多功能细菌纤维素基皮肤敷料及其制备方法 | |
| CN114350728A (zh) | 一种酶法制备透明质酸寡聚糖的方法 | |
| KR101639105B1 (ko) | 히알루론산의 정제 방법 및 제조 방법 | |
| CN115887341A (zh) | 一种红米酶解提取物及制备方法 | |
| AU2020300853B2 (en) | Production of natural organic gluconates | |
| CN105106075B (zh) | 一种具有营养和保湿功效的组合物及其制备方法与应用 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20221008 Address after: 210038 Building 3, No. 5 Xinghe Road, Nanjing Economic and Technological Development Zone, Jiangsu Province Applicant after: Nanjing letao Biotechnology Co.,Ltd. Address before: 210046 building C5, No.9, Weidi Road, Xianlin University Town, Xianlin street, Qixia District, Nanjing City, Jiangsu Province Applicant before: NANJING HANXIN PHARMACEUTICAL TECHNOLOGY Co.,Ltd. |
|
| TA01 | Transfer of patent application right | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20220812 |