CN103484513A - 一种酶法制备小分子寡聚透明质酸的方法 - Google Patents
一种酶法制备小分子寡聚透明质酸的方法 Download PDFInfo
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CN201310498577.5A CN103484513B (zh) | 2013-10-22 | 2013-10-22 | 一种酶法制备小分子寡聚透明质酸的方法 |
US14/251,579 US9279111B2 (en) | 2013-07-29 | 2014-04-12 | Leech hyaluronidase and its application |
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Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103695448A (zh) * | 2013-07-29 | 2014-04-02 | 江南大学 | 一种透明质酸酶编码基因及其发酵生产和纯化方法 |
CN104120158A (zh) * | 2014-07-01 | 2014-10-29 | 江南大学 | 一种添加透明质酸酶提高小分子透明质酸发酵产量的方法 |
CN104178539A (zh) * | 2014-08-19 | 2014-12-03 | 江南大学 | 一种规模化制备特定分子量小分子透明质酸的方法 |
CN104610467A (zh) * | 2015-01-27 | 2015-05-13 | 江南大学 | 一种透明质酸四糖和六糖的分离方法 |
CN105524188A (zh) * | 2015-12-14 | 2016-04-27 | 中国海洋大学 | 一种透明质酸奇数寡糖单体及其制备方法 |
CN106399428A (zh) * | 2016-09-29 | 2017-02-15 | 江南大学 | 一种高效分离制备单一分子量透明质酸寡糖的方法 |
WO2021129353A1 (zh) * | 2019-12-27 | 2021-07-01 | 江苏诚信药业有限公司 | 一种透明质酸水解酶及其编码序列和利用其制备寡聚透明质酸盐的方法 |
CN114181986A (zh) * | 2021-12-16 | 2022-03-15 | 新疆阜丰生物科技有限公司 | 一种低分子透明质酸钠的制备方法 |
CN114288308A (zh) * | 2021-12-02 | 2022-04-08 | 华熙生物科技股份有限公司 | 一种以四糖为主的透明质酸寡糖组合物及其制备方法和应用 |
CN114350728A (zh) * | 2021-08-16 | 2022-04-15 | 华熙生物科技股份有限公司 | 一种酶法制备透明质酸寡聚糖的方法 |
CN114901701A (zh) * | 2019-12-21 | 2022-08-12 | 南京汉欣医药科技有限公司 | 一种超低分子量透明质酸及其制备方法 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103436508A (zh) * | 2013-08-15 | 2013-12-11 | 江南大学 | 一种高效重组表达透明质酸酶的方法 |
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2013
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Patent Citations (1)
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CN103436508A (zh) * | 2013-08-15 | 2013-12-11 | 江南大学 | 一种高效重组表达透明质酸酶的方法 |
Non-Patent Citations (2)
Title |
---|
ROBERT STERN ET AL: "Hyaluronan fragments:an information-rich system", 《EUROPEAN JOURNAL OF CELL BIOLOGY》 * |
杨潼: "关于水蛭透明质酸酶的研究", 《动物学杂志》 * |
Cited By (15)
Publication number | Priority date | Publication date | Assignee | Title |
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CN103695448A (zh) * | 2013-07-29 | 2014-04-02 | 江南大学 | 一种透明质酸酶编码基因及其发酵生产和纯化方法 |
CN104120158A (zh) * | 2014-07-01 | 2014-10-29 | 江南大学 | 一种添加透明质酸酶提高小分子透明质酸发酵产量的方法 |
CN104178539B (zh) * | 2014-08-19 | 2017-11-21 | 江南大学 | 一种规模化制备特定分子量小分子透明质酸的方法 |
CN104178539A (zh) * | 2014-08-19 | 2014-12-03 | 江南大学 | 一种规模化制备特定分子量小分子透明质酸的方法 |
CN104610467A (zh) * | 2015-01-27 | 2015-05-13 | 江南大学 | 一种透明质酸四糖和六糖的分离方法 |
CN105524188A (zh) * | 2015-12-14 | 2016-04-27 | 中国海洋大学 | 一种透明质酸奇数寡糖单体及其制备方法 |
CN105524188B (zh) * | 2015-12-14 | 2018-04-03 | 中国海洋大学 | 一种透明质酸奇数寡糖单体及其制备方法 |
CN106399428A (zh) * | 2016-09-29 | 2017-02-15 | 江南大学 | 一种高效分离制备单一分子量透明质酸寡糖的方法 |
CN106399428B (zh) * | 2016-09-29 | 2019-08-06 | 江南大学 | 一种高效分离制备单一分子量透明质酸寡糖的方法 |
CN114901701A (zh) * | 2019-12-21 | 2022-08-12 | 南京汉欣医药科技有限公司 | 一种超低分子量透明质酸及其制备方法 |
WO2021129353A1 (zh) * | 2019-12-27 | 2021-07-01 | 江苏诚信药业有限公司 | 一种透明质酸水解酶及其编码序列和利用其制备寡聚透明质酸盐的方法 |
CN114350728A (zh) * | 2021-08-16 | 2022-04-15 | 华熙生物科技股份有限公司 | 一种酶法制备透明质酸寡聚糖的方法 |
CN114350728B (zh) * | 2021-08-16 | 2023-10-20 | 华熙生物科技股份有限公司 | 一种酶法制备透明质酸寡聚糖的方法 |
CN114288308A (zh) * | 2021-12-02 | 2022-04-08 | 华熙生物科技股份有限公司 | 一种以四糖为主的透明质酸寡糖组合物及其制备方法和应用 |
CN114181986A (zh) * | 2021-12-16 | 2022-03-15 | 新疆阜丰生物科技有限公司 | 一种低分子透明质酸钠的制备方法 |
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Inventor after: Chen Jian Inventor after: Du Guocheng Inventor after: Kang Zhen Inventor after: Li Jianghua Inventor after: Jin Peng Inventor before: Chen Jian Inventor before: Du Guocheng Inventor before: Kang Zhen Inventor before: Jin Peng |
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Application publication date: 20140101 Assignee: NANJING HANXIN PHARMACEUTICAL TECHNOLOGY Co.,Ltd. Assignor: Jiangnan University Contract record no.: X2020320000330 Denomination of invention: A method of enzymatic preparation of small molecule oligohyaluronic acid Granted publication date: 20150211 License type: Common License Record date: 20201212 |
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Application publication date: 20140101 Assignee: Nanjing letao Biotechnology Co.,Ltd. Assignor: Jiangnan University Contract record no.: X2023980034160 Denomination of invention: A Method for Enzymatic Preparation of Small Molecular Oligomeric Hyaluronic Acid Granted publication date: 20150211 License type: Common License Record date: 20230328 |
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Application publication date: 20140101 Assignee: Shuiyang Cosmetics Manufacturing Co.,Ltd. Assignor: Jiangnan University Contract record no.: X2024980001575 Denomination of invention: A method for enzymatic preparation of small molecule oligomeric hyaluronic acid Granted publication date: 20150211 License type: Common License Record date: 20240130 |