CN113692928B - Breeding method of savatier monochasma herb - Google Patents

Abstract

The invention discloses a breeding method of antlerpilose grass, which comprises the steps of drying harvested fruit pods in the sun, sealing and storing; selecting seeds which are full in particles and free of diseases, insect pests and damage, soaking the seeds in disinfectant, and then cleaning and airing the seeds for later use; selecting a greenhouse, turning the soil layer in the greenhouse, applying a base fertilizer, and moistening the soil layer thoroughly; soaking the seeds obtained in the step two in gibberellin, wherein the soaking process is under natural illumination, and then uniformly broadcasting in a greenhouse; transplanting after the seeds germinate and the seedling plants grow to be 10-20 cm high and after flowers wither and before leaf spreading, wherein the roots of the seedling plants need to be carried with soil during transplanting, and the roots are not damaged as much as possible so as to ensure the survival rate; digging big hole, applying enough thoroughly decomposed organic fertilizer as base fertilizer in the big hole before planting, then loading the seedling with soil into the big hole, sealing soil, and pouring enough water. The invention relates to the technical field of Chinese caterpillar fungus breeding, and particularly provides a breeding method of Chinese caterpillar fungus with high seed germination rate.

Description

Breeding method of savatier monochasma herb

Technical Field

The invention relates to the technical field of Chinese velvet antler grass breeding, in particular to a Chinese velvet antler grass breeding method.

Background

The whole herb of the pilose antler grass is used as the medicine, grows in sandy hills and grasses at the elevation of 100 meters or more, is difficult to meet the requirement of domestic medicine production by excavating wild resources, and also ensures that the natural distribution and the growth supply of the pilose antler grass are less and less.

Disclosure of Invention

Aiming at the situation, the invention provides a method for breeding antlerpilose grass to overcome the defects of the prior art.

The technical scheme adopted by the invention is as follows: a breeding method of pantoea, which comprises the following steps:

step one, storing seeds: uniformly spreading and drying fruit pods harvested after the pilose antler grass is mature, and sealing and storing the fruit pods after drying;

step two, seed screening: selecting seeds which are full in particles and free of diseases, insect pests and damage, soaking the seeds in disinfectant for disinfection, and then cleaning and airing the seeds for later use;

step three, selecting a nursery garden: selecting a greenhouse, controlling the temperature in the greenhouse to be 20-30 ℃ during seedling raising and controlling the illumination time to be 10-12 h;

step four, fertilizing: turning the soil layer in the greenhouse, applying a base fertilizer, and watering and moistening the soil layer thoroughly;

step five, seedling culture: soaking the seeds obtained in the step two in gibberellin, wherein the soaking process is under natural illumination, and then uniformly broadcasting in a greenhouse;

step six, preparation before transplanting: transplanting after the seeds germinate and the seedling plants grow to be 10-20 cm high and after flowers wither and before leaf spreading, wherein the roots of the seedling plants need to be carried with soil during transplanting, and the roots are not damaged as much as possible so as to ensure the survival rate;

step seven, planting field treatment: digging big hole, applying enough thoroughly decomposed organic fertilizer as base fertilizer in the big hole before planting, then loading the seedling with soil into the big hole, sealing soil, and pouring enough water.

Further, the storage temperature is-10 to 5 ℃.

Furthermore, the depth of the plowing is 20-30 cm.

Further, the interval between the adjacent large holes is 20-30 cm.

Furthermore, the disinfectant is a copper sulfate solution with the concentration of 1%, and the soaking time of the disinfectant is 3-7 minutes.

Furthermore, the concentration of the gibberellin is 200-1000 mg/L.

Furthermore, the gibberellin is soaked for 24-48 h.

The invention with the structure has the following beneficial effects: the storage condition adopted by the scheme can ensure the vitality of the seeds to the maximum extent, prolong the service life of the seeds, increase the germination rate of the seeds, and accelerate germination by soaking the seeds with gibberellin, so that the method has the advantages of high germination rate of the seeds, simple operation, easy survival after transplantation and convenient popularization.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments; all other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1, step one, seed storage: uniformly spreading and drying pods harvested after the pilose antler grass is mature, drying, sealing and storing at-10 ℃;

step two, seed screening: selecting seeds which are full and free of diseases, insect pests and damage, soaking the seeds in a copper sulfate solution with the concentration of 1% for disinfection, wherein the soaking time is 3 minutes, and then cleaning and airing for later use;

step three, nursery selection: selecting a greenhouse, controlling the temperature in the greenhouse to be 20 ℃ during seedling raising and controlling the illumination time to be 12h;

step four, fertilizing: turning the soil layer in the greenhouse to the depth of 20cm, applying base fertilizer, and wetting and moistening the soil layer;

step five, seedling culture: soaking the seeds obtained in the step two in 200mg/L gibberellin for 48 hours, wherein the soaking process is under natural illumination, and then uniformly broadcasting in a greenhouse;

step six, preparation before transplanting: transplanting after the seeds germinate and the seedling plants grow to 10cm high and after flowers wither and before leaf unfolding, wherein the roots of the seedling plants need to be carried with soil during transplanting, and the roots are not damaged as much as possible so as to ensure the survival rate;

step seven, planting field treatment: digging big holes, wherein the interval between every two adjacent big holes is 20cm, applying enough thoroughly decomposed organic fertilizer as base fertilizer in the big holes before planting, then loading the seedling plants with soil into the big holes, sealing the soil, and watering enough water.

Example 2, step one, seed storage: uniformly spreading and drying fruit pods harvested after the pilose antler grass is mature, and storing the fruit pods at the temperature of-5 ℃ after drying and sealing;

step two, seed screening: selecting seeds which are full and free of diseases, insect pests and damage, soaking the seeds in a copper sulfate solution with the concentration of 1% for disinfection, wherein the soaking time is 5 minutes, and then cleaning and airing for later use;

step three, nursery selection: selecting a greenhouse, controlling the temperature in the greenhouse to be 25 ℃ during seedling raising and controlling the illumination time to be 11h;

step four, fertilizing: turning the soil layer in the greenhouse to a depth of 25cm, applying base fertilizer, and wetting and moistening the soil layer;

step five, seedling culture: soaking the seeds obtained in the step two in 600mg/L gibberellin for 36 hours, wherein the soaking process is under natural illumination, and then uniformly broadcasting in a greenhouse;

step six, preparation before transplanting: transplanting after the seeds germinate and the seedling plants grow to be 15cm high and after flowers wither and before leaf spreading, wherein the roots of the seedling plants need to be carried with soil during transplanting, and the roots are not damaged as much as possible so as to ensure the survival rate;

step seven, planting field treatment: digging big holes, wherein the interval between every two adjacent big holes is 25cm, applying enough thoroughly decomposed organic fertilizer as base fertilizer in the big holes before planting, then carrying the seedling plants with soil into the big holes, sealing the soil, and watering enough water.

Example 3, step one, seed storage: uniformly spreading and drying fruit pods harvested after the pilose antler grass is mature, and storing the fruit pods at the temperature of 5 ℃ after drying and sealing;

step two, seed screening: selecting seeds which are full and free of diseases, insect pests and damage, soaking the seeds in a copper sulfate solution with the concentration of 1% for disinfection for 7 minutes, and then cleaning and airing for later use;

step three, nursery selection: selecting a greenhouse, controlling the temperature in the greenhouse to be 30 ℃ during seedling culture and controlling the illumination time to be 10h;

step four, fertilizing: turning the soil layer in the greenhouse to the depth of 30cm, applying base fertilizer, and wetting and moistening the soil layer;

step five, seedling culture: soaking the seeds obtained in the step two in 1000mg/L gibberellin for 24 hours, wherein the soaking process is under natural illumination, and then uniformly broadcasting in a greenhouse;

step six, preparation before transplanting: transplanting after the seeds germinate and the seedling plants grow to be 20cm high and after flowers wither and before leaf spreading, wherein the roots of the seedling plants need to be carried with soil during transplanting, and the roots are not damaged as much as possible so as to ensure the survival rate;

step seven, planting field treatment: digging big holes, wherein the interval between every two adjacent big holes is 30cm, applying enough thoroughly decomposed organic fertilizer as base fertilizer in the big holes before planting, then loading the seedling plants with soil into the big holes, sealing the soil, and watering enough water.

Comparative example 1

Providing a breeding method, which is different from the first embodiment in the following steps: uniformly spreading and drying pods harvested after the pilose antler grass is mature, and storing the pods at 10 ℃ after drying and sealing;

all the breeding methods of the embodiments and the control examples of the invention are carried out in the velvet antlers grass base in Yichun Wojiang West.

Comparative example II

Providing a breeding method, wherein different from the first embodiment, the step one is that: uniformly spreading and drying fruit pods harvested after the pilose antler grass is mature, drying, sealing and storing at the temperature of 15 ℃ below zero.

Comparative example III

In this comparative example, the method described in example 2 of the specification of patent No. CN111247972A (a method for harvesting and storing seeds of cornu Cervi Pantotrichum of Shashi) was used to store the Pirophorus pellucorum at-20 deg.C.

Comparative example four

The fourth comparative example provides a propagation method, which is different from the first example in that the second step is seed screening: the method selects seeds with full grains and no plant diseases, insect pests and damage, and does not carry out the step of soaking the seeds in disinfectant for disinfection, cleaning and airing for standby.

Comparative example five

The fifth comparative example provides a propagation method, which is different from the first embodiment in that the fifth step is seedling culture: soaking the seeds obtained in the step two in 100mg/L gibberellin for 48 hours, wherein the soaking process is under natural illumination, and then uniformly broadcasting in a greenhouse;

comparative example six

A sixth comparative example provides a propagation method, which is different from the first embodiment in that the sixth step is to prepare before transplanting: transplanting the seedlings before flowering after the seeds germinate and the seedlings grow to 5-8 cm high, wherein the roots of the seedlings need to be carried with soil during transplanting, and the roots are not damaged as much as possible so as to ensure the survival rate.

Test example 1 germination percentage and survival rate test

The method of the first to third examples and the method of the first to sixth comparative examples are respectively used for breeding the antlerpilose grass, the germination rate of each group of antlerpilose grass is calculated, and the test results are shown in table 1.

Table 1. Calculation results of germination rate and transplanting survival rate of the antlerpilose grass by each formula method.

Group of	Percentage of germination (%)	Survival rate(％)
			Example one	95	93
Example two	90	95
			EXAMPLE III	96	98
Comparative example 1	68	83
			Comparative example II	67	72
Comparative example three	72	75
			Comparative example four	65	65
Comparative example five	72	85
			Comparative example six	90	85

As can be seen from table 1, the steps of seed storage in examples one to three are different from those in comparative examples one to three, and the germination rate and survival rate of examples one to three are significantly higher than those of comparative example one, which proves that the seed storage step has a great influence on the survival rate of transplantation; the germination rate and the survival rate of the disinfection step of the seeds in the fourth removal method of the comparative example are also obviously lower than those of the first to third embodiments, which proves that when the disinfection step of the seeds in the removal method is carried out, the germination rate and the survival rate of the antlerpilose grass are also obviously reduced, the gibberellin concentration of the fifth comparative example is lower, the germination rate and the survival rate of the antlerpilose grass are also obviously reduced, the transplanting is carried out before the flowers of the sixth comparative example, the survival rate is also slightly reduced, the steps of the application have a synergistic effect, and when any one step is modified, the survival rate of the antlerpilose grass is reduced.

It should be noted that, in this document, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (1)

1. The method for breeding the antlerpilose grass is characterized by comprising the following steps of:

step one, storing seeds: uniformly spreading and drying pods harvested after the pilose antler grass is mature, drying, sealing and storing at the temperature of 5 ℃;

step two, seed screening: selecting seeds which are plump and have no diseases, insect pests and damage, soaking the seeds in a copper sulfate solution with the concentration of 1% for disinfection, wherein the soaking time is 7 minutes, and then cleaning and airing for later use;

step three, selecting a nursery garden: selecting a greenhouse, controlling the temperature in the greenhouse to be 30 ℃ during seedling culture and controlling the illumination time to be 10h;

step four, fertilizing: turning the soil layer in the greenhouse to the depth of 30cm, applying base fertilizer, and wetting and moistening the soil layer;

step five, seedling culture: soaking the seeds obtained in the step two in 1000mg/L gibberellin for 24 hours, wherein the soaking process is under natural illumination, and then uniformly broadcasting in a greenhouse;

step six, preparation before transplanting: transplanting after the seeds germinate and the seedling plants grow to be 20cm high and after flowers wither and before leaf spreading, wherein the roots of the seedling plants need to be carried with soil during transplanting, and the roots are not damaged as much as possible so as to ensure the survival rate;

step seven, planting field treatment: digging big holes, wherein the interval between every two adjacent big holes is 30cm, applying enough thoroughly decomposed organic fertilizer as base fertilizer in the big holes before planting, then planting seedlings with soil into the big holes, sealing the soil, and watering enough water.