CN113683712A - 甜菊醇糖苷 - Google Patents

Abstract

本发明涉及甜菊醇糖苷。本发明涉及一种具有式(I)的甜菊醇糖苷，

其中在位置R1上存在至少三个糖部分且在位置R2上存在至少三个糖部分，且其中所述甜菊醇糖苷包括至少七个糖部分，其全部均直接或间接地通过β键联接到甜菊醇糖苷配基。

Description

甜菊醇糖苷

本发明是申请人于2016年4月4日提交的申请号为201680020186.4、题为“甜菊醇糖苷”的中国专利申请的分案申请。

技术领域

本发明涉及甜菊醇糖苷，制备其的方法，包括甜菊醇糖苷的甜味剂组合物、风味组合物、食品、饲料和饮料以及甜菊醇糖苷在甜味剂组合物、风味组合物、食品、饲料和饮料中的用途。

背景技术

多年生草本植物甜叶菊(Stevia rebaudiana Bert.)的叶子积聚大量被称为甜菊醇糖苷的具有强烈甜味的化合物。虽然这些化合物的生物功能尚不清楚，但它们作为替代性高效甜味剂具有商业意义。

这些甜的甜菊醇糖苷的功能和感官特性表现为优于许多高效甜味剂的功能和感官特性。此外，研究表明甜菊苷能够降低II型糖尿病患者的血糖水平，并且能够降低轻度高血压患者的血压。

甜菊醇糖苷积聚在甜叶菊叶中，其中它们可占叶干重的10％至20％。甜菊苷和莱鲍迪甙A均是热和pH稳定的，并且适用于碳酸饮料和许多其他食物。甜菊苷比蔗糖甜110与270倍之间，莱鲍迪甙A比蔗糖甜150与320倍之间。此外，莱鲍迪甙D也是在甜叶菊叶中积聚的高效二萜糖苷甜味剂。它可比蔗糖甜约200倍。莱鲍迪甙M是另一种高效二萜糖苷甜味剂。它在某些甜叶菊品种叶中以痕量存在，但已表明其具有优异的味道特征。

传统上已从甜叶菊植物中提取了甜菊醇糖苷。在甜叶菊中，(-)-贝壳杉烯酸(赤霉酸(GA)生物合成中的中间体)被转化成四环二萜甜菊醇，其然后通过多步糖基化途径进行以形成各种甜菊醇糖苷。然而，产率可以是可变的，并且受到农业和环境条件的影响。此外，甜叶菊种植需要大量的土地面积、在收获前的很长时间、密集劳动以及用于提取和纯化糖苷的额外成本。

但是，仍需要具有替代和/或改善的味道谱的额外的甜菊醇糖苷，这是因为不同的甜菊醇糖苷可更适合于不同的应用。

发明内容

本发明基于从已进行修饰以制备包括rebA的甜菊醇糖苷的微生物获得的发酵液中鉴定新的甜菊醇糖苷。与已知的甜菊醇糖苷相比，新的甜菊醇糖苷将具有不同的感官特性。其可以单独使用或与其他甜菊醇糖苷组合使用，特别是作为甜味剂或用于甜味剂组合物中。

因此，本发明涉及：

-一种具有式(I)的甜菊醇糖苷，

其中在位置R₁上存在至少三个糖部分且在位置R₂上存在至少三个糖部分，且其中甜菊醇糖苷包括至少七个糖部分，其全部均直接或间接地通过β键联接到甜菊醇糖苷配基。

-一种具有式(I)的甜菊醇糖苷，

其中在位置R₁上存在至少四个糖部分且在位置R₂上存在至少三个糖部分。

-一种具有式(I)的甜菊醇糖苷，

其中在位置R₁上存在至少三个糖部分且在位置R₂上存在至少三个糖部分，其中甜菊醇糖苷包括至少七个糖部分，且其中存在于位置R₁的糖中的至少一个通过α键被联接到甜菊醇糖苷配基或糖分子。

-一种具有式(I)的甜菊醇糖苷，

其中在位置R₁上存在至少三个糖部分且在位置R₂上存在至少四个糖部分，其中存在于位置R₂上的糖部分中的至少四个为葡萄糖部分。

-一种具有式(II)的甜菊醇糖苷

-一种具有式(III)的甜菊醇糖苷

-一种具有式(IV)的甜菊醇糖苷

-一种发酵制备的具有式(I)的甜菊醇糖苷，

其中在位置R₁上存在至少三个糖部分且在位置R₂上存在至少三个糖部分，且其中甜菊醇糖苷包括至少七个糖部分；

-一种用于制备根据前述权利要求中任一项所述的甜菊醇糖苷的方法，该方法包括：

提供重组酵母细胞，其包括编码多肽的重组核酸序列，所述多肽包括由下列编码的氨基酸序列：SEQ ID NO：61、SEQ ID NO：65、SEQ ID NO：23、SEQ ID NO：33、SEQ ID NO：77、SEQ ID NO：71、SEQ ID NO：87、SEQ ID NO：73和SEQ ID NO：75；

在合适的发酵培养基中使重组酵母细胞发酵；以及，可选地，

回收根据前述权利要求中任一项所述的甜菊醇糖苷。

-一种组合物，其包括本发明的甜菊醇糖苷以及一种或多种不同的甜菊醇糖苷(其中不同的甜菊醇糖苷可以是或可以不是本发明的甜菊醇糖苷)；

-一种甜味剂组合物、风味组合物、食品、饲料或饮料，其包括本发明的甜菊醇糖苷或组合物；

-本发明的甜菊醇糖苷或组合物在甜味剂组合物或风味组合物中的用途；以及

-本发明的甜菊醇糖苷或组合物在食品、饲料或饮料中的用途。

附图说明

图1显示了质粒pUG7-EcoRV的示意图。

图2显示了将ERG20、tHMG1和BTS1过表达盒设计(A)和整合(B)至酵母基因组中的方法的示意图。(C)示出在通过Cre重组酶移除KANMX标记后的最终情况。

图3示出了ERG9敲低构建体的示意性图示。所述构建体由ERG9的500bp长的3'部分、TRP1启动子的98bp、TRP1开放阅读框和终止子、随后ERG9的400bp长的下游序列组成。由于在ERG9开放阅读框末端处引入Xbal位点，所以最后一个氨基酸变成Ser，并且终止密码子变成Arg。新的终止密码子位于TPR1启动子中，从而导致18个氨基酸的延伸。

图4示出了UGT2如何整合到基因组中的示意性图示。A.在转化中使用的不同片段；B.整合后的情况；C.在Cre重组酶表达后的情况)。

图5示出了从GGPP至RebA的途径如何整合到基因组中的示意性图示。A.在转化中使用的不同片段；B.在整合后的情况。

图6a示出使用高分辨率质谱法，在乙醇提取物(用于纯化的起始物料)中的含有7个葡萄糖(7.1、7.2和7.3)的甜菊醇糖苷混合物的m/z 1451.5820的提取的离子色谱图；且图6b为使用LC-MS的含有7个葡萄糖(7.1、7.2和7.3)的纯化甜菊醇糖苷的m/z 1451.5的提取的离子色谱图。

图7示出莱鲍迪甙7.1的结构。

图8示出莱鲍迪甙7.2的结构。

图9示出莱鲍迪甙7.3的结构。

图10示出莱鲍迪甙M的结构。

图11示出(a)甜菊醇的原子编号以及(b)葡萄糖的原子编号。

图12示出a)Reb M(cdcl3/pyr 1∶1，300K的2滴cdood)，b)Reb 7.1(cdcl3/pyr 1∶3，320K的2滴cdood)，c)Reb 7.2(cdcl3/pyr 1∶1，300K的2滴cdood)和d)Reb 7.3(cdcl3/pyr 1∶2，300K的3滴cdood)的1H NMR谱的选定区域。

序列表的说明

在表15中显示了对序列的描述。本文所述的序列可以参考序列表或参考也显示在表15中的数据库登录号来进行限定。

具体实施方式

在本说明书和所附权利要求书中，词语“包含”、“包括”和“具有”以及变化形式应被解释为包含性的。也就是说，这些词语意图表达在上下文允许的情况下可包含未具体叙述的其他要素或整数。

不使用数量词修饰时在本文中用于指代一个或一个以上(即一个或至少一个)的语法对象。举例来说，“要素”可意指一个要素或多于一个要素。

本发明涉及甜菊醇糖苷。为了本发明的目的，甜菊醇糖苷是甜菊醇的糖苷，特别是其羧基氢原子被葡萄糖分子取代以形成酯以及具有葡萄糖以形成乙缩醛的羟基氢的甜菊醇分子。

可以以分离的形式提供本发明的甜菊醇糖苷。“分离的甜菊醇糖苷”是从可与其天然相关联的其他物料，诸如其他甜菊醇糖苷移出的物质。因此，分离的甜菊醇糖苷可以含有按重量计的至多10％，至多8％，更优选为至多6％，更优选为至多5％，更优选为至多4％，更优选为至多3％，甚至更优选为至多2％，甚至更优选为至多1％，且最优选为至多0.5％的与其天然相关联的其他物料，例如其他甜菊醇糖苷。分离的甜菊醇糖苷可以不含任何其它杂质。本发明的分离的甜菊醇糖苷可以是按重量计的至少50％纯，例如至少60％纯，至少70％纯，至少75％纯，至少80％纯，至少85％纯，至少90％纯，或至少95％、96％、97％、98％、99％、99.5％、99.9％纯。

本发明提供了一种具有式(I)的甜菊醇糖苷，

其中在位置R₁上存在至少三个糖部分且在位置R₂上存在至少三个糖部分，且其中甜菊醇糖苷包括至少七个糖部分，其全部均直接或间接地通过β键联接到甜菊醇糖苷配基，或

其中在位置R₁上存在至少四个糖部分且在位置R₂上存在至少三个糖部分，或

其中在位置R₁上存在至少三个糖部分且在位置R₂上存在至少三个糖部分，其中甜菊醇糖苷包括至少七个糖部分，且其中存在于位置R₁的糖中的至少一个通过α键被联接到甜菊醇糖苷配基或糖分子，或

其中在位置R₁上存在至少三个糖部分且在位置R₂上存在至少四个糖部分，其中存在于位置R₂上的糖部分中的至少四个为葡萄糖部分。

本发明还提供了具有式(II)、(III)或(IV)的甜菊醇糖苷：

本发明的甜菊醇糖苷可以从植物物料获得，但更典型地，将通过发酵制备获得，例如，经对重组宿主细胞诸如酵母细胞的发酵获得。

因此，本发明提供了一种发酵制备的具有式(I)的甜菊醇糖苷，

其中在位置R₁上存在至少三个糖部分且在位置R₂上存在至少三个糖部分，且其中甜菊醇糖苷包括至少七个糖部分。

可以基于糖中的端基异构位置和距离C1最远的立体中心的相对立体化学(R或S)来区分α-和β-糖苷键。通常，当两个碳具有相同的立体化学时，形成α-糖苷键，而当两个碳具有不同的立体化学时，出现β-糖苷键。

这种发酵制备的甜菊醇糖苷可以具有本文所述的甜菊醇糖苷中的任一个的结构。

本发明还涉及一种用于制备甜菊醇糖苷的方法。在这种方法中，在合适的发酵培养基中发酵合适的重组宿主细胞诸如酵母细胞以制备甜菊醇糖苷。可选地，可以回收甜菊醇糖苷。

例如，一种用于制备如本文所述的甜菊醇糖苷的方法可以包括：

提供重组酵母细胞，其包括编码多肽的重组核酸序列，所述多肽包括由下列编码的氨基酸序列：SEQ ID NO：61、SEQ ID NO：65、SEQ ID NO：23、SEQ ID NO：33、SEQ ID NO：77、SEQ ID NO：71、SEQ ID NO：87、SEQ ID NO：73和SEQ ID NO：75；

在合适的发酵培养基中使重组酵母细胞发酵；以及，可选地，

回收如本文所述的甜菊醇糖苷。

在涉及细胞、核酸、蛋白质或载体使用时，术语“重组”指示细胞、核酸、蛋白质或载体已通过引入异源核酸或蛋白质或改变天然核酸或蛋白质来进行修饰，或者指示细胞源自如此修饰的细胞。因此，例如，重组细胞表达在细胞的天然(非重组)形式中未发现的基因或者表达以其他形式异常表达、低表达或完全未表达的天然基因。术语“重组的”与“遗传修饰的”同义。

用于本发明的方法中的重组酵母细胞可以是任何合适的酵母细胞。优选的重组酵母细胞可以选自下列各属：酵母属(Saccharomyces)(例如，酿酒酵母(S.cerevisiae)、贝酵母(S.bayanus)、巴斯德酵母(S.pastorianus)、卡尔斯伯酵母(S.carlsbergensis))、酒香酵母属(Brettanomyces)、克鲁维酵母属(Kluyveromyces)、假丝酵母属(Candida)(例如，克鲁斯假丝酵母(C.krusei)、拉考夫假丝酵母(C.revkaufi)、铁红假丝酵母(C.pulcherrima)、热带假丝酵母(C.tropicalis)、产朊假丝酵母(C.utilis))、伊萨酵母属(Issatchenkia)(例如，东方伊萨酵母(I.orientalis))、毕赤酵母属(Pichia)(例如，巴斯德毕赤酵母(P.pastoris))、裂殖酵母属(Schizosaccharomyces)、汉逊酵母属(Hansenula)、克勒克酵母属(Kloeckera)、管囊酵母属(Pachysolen)、许旺酵母属(Schwanniomyces)、毛孢子菌属(Trichosporon)、耶氏酵母属(Yarrowia)(例如，解脂耶氏酵母(Y.lipolytica)(先前分类为解脂假丝酵母(Candida lipolytica)))、Yamadazyma。优选地，重组酵母细胞是酿酒酵母、解脂耶氏酵母或东方伊萨酵母细胞。

用于根据本发明所述方法中的重组酵母细胞可以包括一个或多个重组核苷酸序列，其对下列中的一个或多个进行编码：

具有对映-柯巴基焦磷酸合酶活性的多肽；

具有对映-贝壳杉烯合酶活性的多肽；

具有对映-贝壳杉烯氧化酶活性的多肽；以及

具有贝壳杉烯酸-13-羟化酶活性的多肽。

出于本发明的目的，具有对映-柯巴基焦磷酸合酶(EC 5.5.1.13)的多肽能够催化化学反应：

所述酶具有一种底物，香叶基香叶基焦磷酸；以及一种产物，对映-柯巴基焦磷酸。所述酶参与赤霉素生物的合成。所述酶属于异构酶家族，特别是分子内裂解酶的类别。所述酶类别的系统名称是对映-柯巴基-二磷酸裂解酶(脱环)。通常使用的其他名称包括具有对映-柯巴基焦磷酸合酶、对映-贝壳杉烯合酶A和对映-贝壳杉烯合成酶A。

编码对映-柯巴基焦磷酸合酶的合适核酸序列可例如包含在SEQ ID.NO：1、3、5、7、17、19、59、61、141、142、151、152、153、154、159、160、182或184中列出的序列。

出于本发明的目的，具有对映-贝壳杉烯合酶活性(EC 4.2.3.19)的多肽是能够催化以下化学反应的多肽：

对映-柯巴基二磷酸

对映-贝壳杉烯+二磷酸

因此，所述酶具有一种底物，对映-柯巴基二磷酸；以及两种产物，对映-贝壳杉烯和二磷酸。

所述酶属于裂解酶家族，特别是作用于磷酸盐/酯的碳-氧裂解酶。所述酶类别的系统名称是对映-柯巴基二磷酸二磷酸-裂解酶(环化，对映-贝壳杉烯形成)。常用的其它名称包括对映-贝壳杉烯合酶B、对映-贝壳杉烯合成酶B、对映-柯巴基-二磷酸二磷酸-裂解酶和(环化)。所述酶参与双萜类生物合成。

编码对映-贝壳杉烯合酶的合适核酸序列可例如包含在SEQ ID.NO：9、11、13、15、17、19、63、65、143、144、155、156、157、158、159、160、183或184中列出的序列。

对映-柯巴基二磷酸合酶还可具有与相同蛋白质分子相关联的不同对映-贝壳杉烯合酶活性。由对映-贝壳杉烯合酶催化的反应是赤霉素的生物合成途径中的下一步骤。两种类型的酶活性是不同的，并且定点诱变以抑制蛋白质的对映-贝壳杉烯合酶活性导致对映-柯巴基焦磷酸的积累。

因此，在适用于本发明方法的重组酵母中使用的单个核苷酸序列可编码具有对映-柯巴基焦磷酸合酶活性和对映-贝壳杉烯合酶活性的多肽。或者，两种活性可被两个不同的分离的核苷酸序列编码。

出于本发明的目的，具有对映-贝壳杉烯氧化酶活性(EC 1.14.13.78)的多肽是能够催化对映-贝壳杉烯的4-甲基的三次连续氧化以产生贝壳杉烯酸的多肽。这种活性通常需要细胞色素P450的存在。

编码对映-贝壳杉烯氧化酶的合适核酸序列可例如包含在SEQ ID.NO：21、23、25、67、85、145、161、162、163、180或186中列出的序列。

出于本发明的目的，具有贝壳杉烯酸13-羟化酶活性(EC 1.14.13)的多肽是能够催化使用NADPH和O₂形成甜菊醇(对映-贝壳杉-16-烯-13-醇-19-酸)的多肽。这种活性也可称为对映-贝壳杉烯酸13-羟化酶活性。

编码贝壳杉烯酸13-羟化酶的合适核酸序列可例如包含在SEQ ID.NO：27、29、31、33、69、89、91、93、95、97、146、164、165、166、167或185中列出的序列。

适用于本发明方法的重组酵母细胞可包含编码具有NADPH-细胞色素p450还原酶活性的多肽的重组核酸序列。也就是说，适用于本发明方法的重组酵母可能够表达编码具有NADPH-细胞色素p450还原酶活性的多肽的核苷酸序列。出于本发明的目的，具有NADPH-细胞色素P450还原酶活性(EC 1.6.2.4；也称为NADPH：高铁血红蛋白氧化还原酶、NADPH：血红素蛋白氧化还原酶、NADPH：P450氧化还原酶、P450还原酶、POR、CPR、CYPOR)的多肽通常是一种这样的多肽，其为膜结合酶，从而允许电子从含有FAD和FMN的酶NADPH：细胞色素P450还原酶(POR；EC 1.6.2.4)转移至真核细胞的微粒体中的细胞色素P450。

编码NADPH-细胞色素p450还原酶的合适的核酸序列可以例如包括在SEQ ID.NO：53、55、57或77中显示的序列。

适合用于本发明的方法中的重组酵母细胞还可以包括一个或多个重组核酸序列，其对下列中的一个或多个进行编码：

(i)具有UGT74G1活性的多肽；

(ii)具有UGT2活性的多肽；

(iii)具有UGT85C2活性的多肽；以及

(iv)具有UGT76G1活性的多肽。

适合用于本发明中的重组酵母可以包括编码能够催化将C-13-葡萄糖添加至甜菊醇的多肽的核苷酸序列。也就是说，适合用于本发明的方法中的重组酵母可以包括UGT，其能够催化其中将甜菊醇转化成甜菊单糖苷的反应。

这种适合用于本发明方法中的重组酵母可包含编码具有由UDP-糖基转移酶(UGT)UGT85C2所示的活性的多肽的核苷酸序列，由此酵母转化后的核苷酸序列赋予所述酵母将甜菊醇转化为甜菊醇单糖苷的能力。

UGT85C2活性是将葡萄糖单元转移至甜菊醇的13-OH。因此，合适的UGT85C2可充当尿苷5'-二磷酸葡糖基∶甜菊醇13-OH转移酶和尿苷5'-二磷酸葡糖基∶甜菊醇-19-O-糖苷13-OH转移酶。功能性UGT85C2多肽还可催化葡糖基转移酶反应，所述反应利用除甜菊醇和甜菊醇-19-O-糖苷以外的甜菊醇糖苷底物。此类序列可在本文中称为UGT1序列。

适合用于本发明中的重组酵母可以包括编码具有UGT2活性的多肽的核苷酸序列。

具有UGT2活性的多肽是用作尿苷5’-二磷酸葡糖基：甜菊醇-13-O-葡萄糖苷转移酶(也称为甜菊醇-13-单葡萄糖苷1，2-转葡糖基酶)的多肽，其将葡萄糖部分转移至受体分子甜菊醇-13-O-葡萄糖苷的13-O-葡萄糖的C-2’。通常，合适的UGT2多肽也用作尿苷5’-二磷酸葡糖基：甜茶苷转移酶，其将葡萄糖部分转移到受体分子甜茶苷的13-O-葡萄糖的C-2’。

具有UGT2活性的多肽还可以催化利用除了甜菊醇-13-O-葡萄糖苷和甜茶苷以外的甜菊醇糖苷底物的反应，例如，功能性UGT2多肽可以利用甜菊苷作为底物，将葡萄糖部分转移到19-O-葡萄糖残基的C-2’以制备莱鲍迪甙E。功能性UGT2多肽还可以利用莱鲍迪甙A作为底物，将葡萄糖部分转移到19-O-葡萄糖残基的C-2’以制备莱鲍迪甙D。然而，功能性UGT2多肽通常不将葡萄糖部分转移到在C-13位置具有1，3-结合葡萄糖的甜菊醇化合物，即通常不发生葡萄糖部分至甜菊醇1，3-双糖苷和1，3-甜菊苷的转移。

具有UGT2活性的多肽也可以将糖部分从除了尿苷二磷酸葡萄糖以外的供体进行转移。例如，具有UGT2活性的多肽充当尿苷5’-二磷酸D-木糖基：甜菊醇-13-O-葡萄糖苷转移酶，其将木糖部分转移到受体分子甜菊醇-13-O-葡萄糖苷的13-O-葡萄糖的C-2’。作为另一个实例，具有UGT2活性的多肽可以充当尿苷5’-二磷酸L-鼠李糖基：甜菊醇-13-O-葡萄糖苷转移酶，其将鼠李糖部分转移到受体分子甜菊醇的13-O-葡萄糖的C-2’。

适合用于本发明的方法中的重组酵母可以包括编码具有UGT活性的核苷酸序列，可以包括编码能够催化将C-19-葡萄糖添加至甜菊双糖苷的多肽的核苷酸序列。也就是说，本发明的重组酵母可以包括UGT，其能够催化其中将甜菊双糖苷转化成甜菊苷的反应。因此，这样的重组酵母可能够将甜菊双糖苷转化成甜菊苷。这种核苷酸序列的表达可以赋予重组酵母制备至少甜菊苷的能力。

适合用于本发明的方法中的重组酵母因此还可以包括编码具有由UDP-糖基转移酶(UGT)UGT74G1所示活性的多肽的核苷酸序列，由此在进行酵母转化后核苷酸序列赋予该细胞将甜菊双糖苷转化成甜菊苷的能力。

合适的UGT74G1多肽可能够将葡萄糖单元分别转移至甜菊醇的13-OH或19-COOH。合适的UGT74G1多肽可充当尿苷5'-二磷酸葡糖基∶甜菊醇19-COOH转移酶和尿苷5'-二磷酸葡糖基∶甜菊醇-13-O-糖苷19-COOH转移酶。功能性UGT74G1多肽还可催化使用除甜菊醇和甜菊醇-13-O-糖苷以外的甜菊醇糖苷底物或者从除尿苷二磷酸葡萄糖以外的供体转移糖部分的糖基转移酶反应。此类序列可在本文中称为UGT3序列。

适合用于本发明的方法中的重组酵母可包含编码能够催化甜菊苷的C-13位置处的葡萄糖的C-3'的葡糖基化的多肽的核苷酸序列。也就是说，适合用于本发明的方法中的重组酵母可包含UGT，所述UGT能够催化甜菊苷至莱鲍迪甙A的反应。因此，这种重组酵母可能够将甜菊苷转化为莱鲍迪甙A。这种核苷酸序列的表达可赋予酵母产生至少莱鲍迪甙A的能力。

因此，适合用于本发明的方法中的重组酵母还可包含编码具有由UDP-糖基转移酶(UGT)UGT76G1所示的活性的多肽的核苷酸序列，由此酵母转化后的核苷酸序列赋予酵母将甜菊苷转化为莱鲍迪甙A的能力。

合适的UGT76G1向受体分子甜菊醇1,2糖苷的C-13-O-葡萄糖的C-3'添加葡萄糖部分。因此，UGT76G1例如充当尿苷5'-二磷酸葡糖基∶甜菊醇13-O-1,2葡糖苷C-3'葡糖基转移酶和尿苷5'-二磷酸葡糖基∶甜菊醇-19-O-葡萄糖、13-O-1,2双糖苷C-3'葡糖基转移酶。功能性UGT76G1多肽还可催化葡糖基转移酶反应，所述反应使用含有除葡萄糖以外的糖的甜菊醇糖苷底物，例如甜菊醇鼠李糖苷和甜菊醇木糖苷。此类序列可在本文中称为UGT4序列。UGT4可以替代地或额外地能够将RebD转化成RebM。

适合用于本发明的方法中的重组酵母通常包括编码至少一种具有UGT1活性的多肽，至少一种具有UGT2活性的多肽，至少一种具有UGT3活性的多肽和至少一种具有UGT4活性的多肽的核苷酸序列。这些核酸序列中的一种或多种可以是重组的。给定的核酸可以编码具有上述活性中的一组或多种的多肽。例如，核酸编码具有上述活性中的两种、三种或四种的多肽。优选地，用于本发明的方法中的重组酵母包括UGT1、UGT2、UGT3和UGT4活性。在本文的表15中描述了合适的UGT1、UGT2、UGT3和UGT4序列。编码UGT1、2、3和4活性的序列的优选组合为SEQ ID NO：71、87、73和75。

在本发明的方法中，重组宿主例如酵母可以能够在本领域中已知的任何合适的碳源上生长，并且将其转化为一种或更多种甜菊醇糖苷。重组宿主可能够直接转化植物生物质、纤维素、半纤维素、果胶、鼠李糖、半乳糖、岩藻糖、麦芽糖、麦芽糖糊精、核糖、核酮糖或淀粉、淀粉衍生物、蔗糖、乳糖和甘油。因此，优选的宿主表达酶如用于将纤维素转化成葡萄糖单体和将半纤维素转化成木糖和阿拉伯糖单体所需的纤维素酶(内切纤维素酶和外切纤维素酶)和半纤维素酶(例如内切和外切木聚糖酶、阿拉伯糖酶)，能够将果胶转化成葡萄糖醛酸和半乳糖醛酸的果胶酶或将淀粉转化成葡萄糖单体的淀粉酶。优选地，宿主能够转化选自由以下各项组成的组的碳源：葡萄糖、木糖、阿拉伯糖、蔗糖、乳糖和甘油。宿主细胞可例如是WO03/062430、WO06/009434、EP1499708B1、WO2006096130或WO04/099381中所描述的真核宿主细胞。

在用于产生本发明的甜菊醇糖苷的方法中使用的发酵培养基可以是允许特定真核宿主细胞生长的任何合适的发酵培养基。发酵培养基的基本要素是本领域的技术人员已知的，并且可适用于所选择的宿主细胞。

优选地，发酵培养基包含选自由以下各项组成的组的碳源：植物生物质、纤维素、半纤维素、果胶、鼠李糖、半乳糖、岩藻糖、果糖、麦芽糖、麦芽糖糊精、核糖、核酮糖或淀粉、淀粉衍生物、蔗糖、乳糖、脂肪酸、甘油三酯和甘油。优选地，发酵培养基还包含氮源，如尿素；或铵盐，如硫酸铵、氯化铵、硝酸铵或磷酸铵。

根据本发明的发酵方法可以分批、分批补料或连续模式进行。也可应用单独的水解和发酵(SHF)方法或同时糖化和发酵(SSF)方法。这些发酵方法模式的组合对于最佳生产率来说也可以是可行的。如果在发酵方法中使用淀粉、纤维素、半纤维素或果胶作为碳源，则SSF方法可以是特别有吸引力的，其中可需要添加水解酶如纤维素酶、半纤维素酶或果胶酶以水解底物。

用于产生根据本发明的甜菊醇糖苷的发酵方法可以是需氧或厌氧发酵方法。

厌氧发酵方法可在本文中定义为在不存在氧的情况下运行或者基本上不消耗氧(优选小于5、2.5或1mmol/L/h)，并且其中有机分子充当电子供体和电子受体两者的发酵方法。根据本发明的发酵方法也可首先在需氧条件下运行，且随后在厌氧条件下运行。

发酵方法也可在限氧或微需氧条件下进行。或者，发酵方法可首先在需氧条件下运行，且随后在限氧条件下运行。限氧发酵方法是其中氧消耗受到从气体到液体的氧传递的限制的过程。氧限制的程度由进入气流的量和组成以及所用发酵设备的实际混合/传质特性决定。

在根据本发明的方法中产生甜菊醇糖苷可在宿主细胞的生长阶段期间、固定(稳定状态)阶段期间或在两个阶段期间发生。在不同的温度下运行发酵方法可以是可行的。

用于产生甜菊醇糖苷的方法可在对于重组宿主来说最佳的温度下进行。对于每种转化的重组宿主而言，最佳生长温度可不同并且是本领域的技术人员已知的。最佳温度可高于野生型生物的最适温度以在非无菌条件下在最低感染敏感性和最低冷却成本的条件下有效生长生物体。或者，所述方法可在对于重组宿主的生长来说不是最佳的温度下进行。

用于产生根据本发明的甜菊醇糖苷的方法可在任何合适的pH值下进行。如果重组宿主是酵母，则发酵培养基中的pH优选具有低于6、优选低于5.5、优选低于5、优选低于4.5、优选低于4、优选低于pH 3.5或低于pH3.0或低于pH 2.5、优选高于pH 2的值。在这些低pH值下进行发酵的优点是可防止发酵培养基中污染细菌的生长。

这种方法可以以工业规模进行。这种方法的产物是根据本发明所述的一种或多种甜菊醇糖苷。

从发酵培养基回收本发明的甜菊醇糖苷可以通过本领域中已知的方法，例如通过蒸馏、真空提取、溶剂提取或蒸发来执行。

在根据本发明所述的用于制备甜菊醇糖苷的方法中，可以实现高于0.5mg/l，优选为高于约1mg/l的浓度。

在重组宿主中表达本发明的一种或多种甜菊醇糖苷的情况下，这种细胞可需要进行处理以将其释放。

本发明还提供了一种组合物，其包括与一种或多种不同的甜菊醇糖苷相组合的本发明的甜菊醇糖苷。一种或多种不同的甜菊醇糖苷中的一种或多种可以是本发明的甜菊醇糖苷。一种或多种不同的甜菊醇糖苷中的一种或多种可以是糖基化二萜(即，二萜糖苷)，诸如甜菊单糖苷、甜菊双糖苷、甜菊苷、莱鲍迪甙A、莱鲍迪甙B、莱鲍迪甙C、莱鲍迪甙D、莱鲍迪甙E、莱鲍迪甙F、莱鲍迪甙M、甜茶苷、杜尔可苷A、甜菊醇-13-单糖苷、甜菊醇-19-单糖苷或13-[(β-D-吡喃葡萄糖基)氧基)贝壳杉-16-烯-18-酸2-O-β-D-吡喃葡萄糖基-β-D-吡喃葡萄糖基酯。

本发明的组合物可以包括与更大量的不同的甜菊醇糖苷相组合的相对少量的本发明的甜菊醇糖苷。

例如，本发明的组合物可以包括与本发明的甜菊醇糖苷相组合的至少约80％、至少约90％、至少约95％的莱鲍迪甙A。本发明的组合物可以包括与本发明的甜菊醇糖苷相组合的至少约80％、至少约90％、至少约95％的莱鲍迪甙D。本发明的组合物可以包括与本发明的甜菊醇糖苷相组合的至少约80％、至少约90％、至少约95％的莱鲍迪甙M。本发明的组合物可以包括与本发明的甜菊醇糖苷和莱鲍迪甙D相组合的至少约80％、至少约90％、至少约95％的莱鲍迪甙A。本发明的组合物可以包括与本发明的甜菊醇糖苷和莱鲍迪甙M相组合的至少约80％、至少约90％、至少约95％的莱鲍迪甙A。所提及的百分比是以干重计的。

根据本发明所述的甜菊醇糖苷可以用于已知的用于这种化合物的任何应用中。特别地，其可以例如用作甜味剂或风味剂，例如在食品、饲料或饮料中。例如，甜菊醇糖苷可以被配制在软饮料诸如碳酸饮料、桌面甜味剂、口香糖、乳制品诸如酸奶(例如，原味酸奶)、蛋糕、谷物或基于谷物的食品、营养食品、药物、食用凝胶、糖食、化妆品、牙膏或其他口腔组合物等中。此外，甜菊醇糖苷可以用作甜味剂，其不仅可用于饮料、食品和其他专用于人类消费的制品中，还可用于具有改善的特性的动物饲料和草料中。

因此，本发明尤其提供了一种甜味剂组合物、风味剂组合物、食品、饲料或饮料，其包括根据本发明的方法所制备的甜菊醇糖苷。

本发明的组合物可以包括一种或多种非天然存在的组分。

而且，本发明提供了：

-本发明的甜菊醇糖苷或组合物在甜味剂组合物或风味组合物中的用途；以及

-本发明的甜菊醇糖苷或组合物在食品、饲料或饮料中的用途。

在制造食品、饮料、药物、化妆品、桌面制品、口香糖期间，可以使用传统的方法，诸如混合、捏合、溶解、浸酸、渗透、渗滤、喷洒、雾化、注入以及其他方法。

在本发明中获得的甜菊醇糖苷能够以干或液体形式使用。其能够在对食品进行热处理前或后进行添加。甜味剂的量取决于使用目的。其能够单独地或与其他化合物相组合地进行添加。

根据本发明的方法制备的化合物可以与一种或多种另外的非热量或热量甜味剂相混合。这种混合可以用于改善风味或时间特性或稳定性。本发明的甜菊醇糖苷可以用于改善第二种甜菊醇糖苷，诸如莱鲍迪甙A、D或M的风味或时间特性或稳定性。

大范围的非热量或热量甜味剂可以适合于与本发明的甜菊醇糖苷，包括根据本发明所述的一种或多种其他甜菊醇糖苷或一种或多种其他已知的甜菊醇糖苷，诸如甜菊单糖苷、甜菊双糖苷、甜菊苷、莱鲍迪甙A、莱鲍迪甙B、莱鲍迪甙C、莱鲍迪甙D、莱鲍迪甙E、莱鲍迪甙F、莱鲍迪甙M、甜茶苷、杜尔可苷A、甜菊醇-13-单糖苷、甜菊醇-19-单糖苷或13-[(β-D-吡喃葡萄糖基)氧基)贝壳杉-16-烯-18-酸2-O-β-D-吡喃葡萄糖基-β-D-吡喃葡萄糖基酯相混合。替代地或额外地，非热量甜味剂为诸如罗汉果苷、莫纳甜、阿斯巴甜、安赛蜜盐、甜蜜素、三氯蔗糖、糖精盐或赤藓糖醇。适合与甜菊醇糖苷相混合的热量甜味剂包括糖醇和碳水化合物，诸如蔗糖、葡萄糖、果糖和HFCS。还可以使用甜味氨基酸，诸如甘氨酸、丙氨酸或丝氨酸。

甜菊醇糖苷可与甜味剂抑制剂如天然甜味剂抑制剂组合使用。它可与鲜味增强剂如氨基酸或其盐组合。

甜菊醇糖苷可与多元醇或糖醇、碳水化合物、生理活性物质或功能成分(例如类胡萝卜素、膳食纤维、脂肪酸、皂苷、抗氧化剂、营养食品、类黄酮、异硫氰酸酯、苯酚、植物甾醇或甾烷醇(植物甾醇和植物甾烷醇)、多元醇、益生元、益生菌、植物雌激素、大豆蛋白、硫化物/硫醇、氨基酸、蛋白质、维生素、矿物质和/或基于健康益处如心血管、降胆固醇或抗炎分类的物质组合。

具有甜菊醇糖苷的组合物可包括调味剂、芳香组分、核苷酸、有机酸、有机酸盐、无机酸、苦味化合物、蛋白质或蛋白质水解产物、表面活性剂、类黄酮、收敛剂化合物、维生素、膳食纤维、抗氧化剂、脂肪酸和/或盐。

本发明的甜菊醇糖苷可作为高强度甜味剂应用，以产生具有改进的味道特征的零卡路里、低卡路里或糖尿病人用饮料和食品。它也可用于不能使用糖的饮料、食品、药物和其他产品中。

此外，本发明的甜菊醇糖苷可用作甜味剂，不仅用于饮料、食品和其它专门用于人消费的产品，而且用于具有改进的特性的动物饲料和草料中。

本发明组合物的甜菊醇糖苷可用作甜味化合物的产品的实例可以是酒精饮料，如伏特加酒、葡萄酒、啤酒、烈酒、清酒等；天然果汁、提神饮料、碳酸软饮料、减肥饮料、零卡路里饮料、低卡路里饮料和食物、酸奶饮料、速溶果汁、速溶咖啡、粉末型速溶饮料、罐装产品、糖浆、发酵大豆酱、酱油、醋、调味品、蛋黄酱、番茄酱、咖喱、汤、速食肉汤、酱油粉、醋粉、多种类型的饼干、香米饼、咸饼干、面包、巧克力、焦糖、糖果、口香糖、果冻、布丁、蜜饯和腌菜、鲜奶油、果酱、橘子酱、糖花膏、奶粉、冰淇淋、冰糕、包装在瓶中的蔬菜和水果、罐装和煮熟的豆类、在甜味酱中煮熟的肉和食物、农业蔬菜食品、海鲜、火腿、香肠、鱼火腿、鱼香肠、鱼酱、油炸鱼制品、干制海产品、冷冻食品、腌渍海带、腊肉、烟草、医药产品等。原则上它可具有无限应用。

甜味组合物包含饮料，其非限制性实例包括非碳酸化和碳酸饮料，如可乐、姜汁汽水、根汁汽水、苹果汁、水果味软饮料(例如柑橘味软饮料，如柠檬莱姆或橙汁)、软饮料粉等；来自水果或蔬菜的果汁、包括榨汁等的果汁、含有果粒的果汁、水果饮料、果汁饮料、含果汁的饮料、具有水果调味料的饮料、蔬菜汁、含蔬菜的汁以及含水果和蔬菜的混合果汁；运动饮料、能量饮料、接近水的饮料等(例如具有天然或合成调味剂的水)；茶类或喜欢型饮料如咖啡、可可、红茶、绿茶、乌龙茶等；含乳成分饮料如乳饮料、含乳成分咖啡、牛奶咖啡、奶茶、果奶饮料、饮用酸奶、乳酸菌饮料等；以及乳制品。

通常，甜味组合物中存在的甜味剂的量取决于甜味组合物的具体类型及其所需的甜度而广泛变化。本领域的普通技术人员可容易确定加入到甜味组合物中的甜味剂的适当量。

本发明的甜菊醇糖苷可以干或液体形式使用。它可在食品热处理之前或之后加入。甜味剂的量取决于使用目的。它可单独添加或与其它化合物组合添加。

在食品、饮料、药物、化妆品、桌面产品、口香糖的制造过程中，可使用诸如混合、捏合、溶解、酸洗、渗透、渗滤、喷洒、雾化、灌注和其它方法的常规方法。

因此，本发明的组合物可通过本领域的技术人员已知的提供成分的均匀或均质混合物的任何方法来制备。这些方法包括干混、喷雾干燥、团聚、湿法制粒、压实、共结晶等。

呈固体形式时，本发明的甜菊醇糖苷可以适于递送到待甜化的食物中的任何形式提供给消费者，所述形式包括小袋、小包、散装袋或盒、方块、片剂、喷雾或可溶解的条。所述组合物可以单位剂量或散装形式递送。

对于液体甜味剂体系和组合物而言，应开发方便范围的流体、半流体、糊状和膏状形式、使用任何形状或形式的适当包装材料的适当包装，其便于携带或分配或储存或运输含有任何上述甜味剂产品或上述产品的组合的任何组合。

所述组合物可包含多种填充剂、功能成分、着色剂、调味剂。

标准遗传技术，诸如在宿主细胞中的酶的过表达、宿主细胞的遗传修饰或杂交技术是本领域中的已知方法，诸如在Sambrook和Russel(2001)的“分子克隆：实验室手册(第3版)”，冷泉港实验室，冷泉港实验室出版社，或F.Ausubel等人编辑，“最新分子生物学实验方法汇编”，Green Publishing and Wiley Interscience，纽约(1987)中所述的。根据例如EP-A-0635574、WO 98/46772、WO 99/60102和WO 00/37671、WO90/14423、EP-A-0481008、EP-A-0635574和US 6265186，已知用于真菌宿主细胞的转化、遗传修饰等的方法。

本发明的一些实施方案：

1.一种具有式(I)的甜菊醇糖苷，

其中在位置R₁上存在至少三个糖部分且在位置R₂上存在至少三个糖部分，且其中甜菊醇糖苷包括至少七个糖部分，其全部均直接或间接地通过β键联接到甜菊醇糖苷配基。

2.一种具有式(I)的甜菊醇糖苷，

其中在位置R₁上存在至少四个糖部分且在位置R₂上存在至少三个糖部分。

3.一种具有式(I)的甜菊醇糖苷，

其中在位置R₁上存在至少三个糖部分且在位置R₂上存在至少三个糖部分，其中甜菊醇糖苷包括至少七个糖部分，且其中存在于位置R₁的糖中的至少一个通过α键被联接到甜菊醇糖苷配基或糖分子。

4.一种具有式(I)的甜菊醇糖苷，

其中在位置R₁上存在至少三个糖部分且在位置R₂上存在至少四个糖部分，其中存在于位置R₂上的糖部分中的至少四个为葡萄糖部分。

5.一种具有式(II)的甜菊醇糖苷

6.一种具有式(III)的甜菊醇糖苷

7.一种具有式(IV)的甜菊醇糖苷

8.根据前述实施方案中任一项所述的甜菊醇糖苷，其为发酵制备的。

9.发酵制备的具有式(I)的甜菊醇糖苷，

其中在位置R₁上存在至少三个糖部分且在位置R₂上存在至少三个糖部分，且其中甜菊醇糖苷包括至少七个糖部分。

10.根据实施方案9所述的甜菊醇糖苷，其具有根据实施方案1至7中的任一项的结构。

11.一种用于制备根据前述实施方案中任一项所述的甜菊醇糖苷的方法，所述方法包括：

提供重组酵母细胞，其包括编码多肽的重组核酸序列，所述多肽包括由下列编码的氨基酸序列：SEQ ID NO：61、SEQ ID NO：65、SEQ ID NO：23、SEQ ID NO：33、SEQ ID NO：59、SEQ ID NO：71、SEQ ID NO：87、SEQ ID NO：73和SEQ ID NO：75；

在合适的发酵培养基中发酵重组酵母细胞；以及，可选地，

回收根据前述实施方案中任一项所述的甜菊醇糖苷。

12.一种组合物，其包括根据实施方案1至11中的任一项所述的甜菊醇糖苷以及一种或多种不同的甜菊醇糖苷。

13.一种食品、饲料或饮料，其包括根据实施方案1至10中的任一项所述的甜菊醇糖苷或根据实施方案12所述的组合物。

14.根据实施方案1至10中的任一项所述的甜菊醇糖苷或根据实施方案12所述的组合物在甜味剂组合物或风味组合物中的用途。

15.根据实施方案1至10中的任一项所述的甜菊醇糖苷或根据实施方案12所述的组合物在食品、饲料或饮料中的用途。

本文中对作为现有技术而给出的专利文件或其他事项的参考不应被视为承认该文件或事项是已知的或其含有的信息是在权利要求中的任一项的优先权日的公知常识的一部分。

本文阐明的每个参考文献的公开内容均通过引用整体并入本文。

本发明还通过下列实施例进一步进行了说明。

实施例

实施例1：STV016的构建

构建酿酒酵母菌株STV016以用于甜菊醇糖苷的发酵制备。

1.1 ERG20、BTS1和tHMG在酿酒酵母中的过表达

对于ERG20、BTS1 tHMG1的过度表达而言，使用W02013/076280中描述的技术将表达盒设计为整合在一个基因座中。为了扩增整合基因座的5'和3'整合侧翼，使用了来自CEN.PK酵母菌株(van Dijken等人.Enzyme and Microbial Technology 26(2000)706-714)的合适的引物和基因组DNA。不同的基因在DNA2.0作为盒(含有同源序列、启动子、基因、终止子、同源序列)订购。这些盒中的基因侧接组成型启动子和终止子。参见表1。将来自DNA2.0的含有ERG20、tHMG1和BTS1盒的质粒DNA溶解至100ng/μl的浓度。在50μl PCR混合物中，20ng模板与20pmol的引物一起使用。将材料溶解至0.5μg/μl的浓度。

表1过表达构建体的组成

为了扩增选择标记，使用了pUG7-EcoRV构建体(图1)和合适的引物。使用Zymoclean凝胶DNA回收试剂盒(ZymoResearch)从凝胶中纯化KanMX片段。将酵母菌株Cen.PK113-3C用表2中所列的片段转化。

表2用于ERG20、tHMG1和BTS1转化的DNA片段

片段
	5’YPRcTau3
ERG20盒
	tHMG1盒
KanMX盒
	BTS1盒
3’YPRcTau3

在30℃下在YEPhD(酵母提取物植物蛋白胨葡萄糖；来自BD的BBL植物蛋白胨)中转化和恢复2.5小时后，将细胞与200μg/ml G418(Sigma)一起接种在YEPhD琼脂上。将板在30℃下孵育4天。通过诊断PCR和测序确定正确的整合。用蛋白质上LC/MS证实过度表达。图2中示出了ERG20、tHMG1和BTS1的组装示意图。此菌株被命名为STV002。

此菌株中CRE-重组酶的表达导致KanMX标记的外重组。用诊断PCR确定ERG20、tHMG和BTS1的存在和正确外重组。

1.2 Erg9的敲低

为了降低Erg9的表达，设计并使用了Erg9敲低构建体，所述构建体含有修饰的3'端，其继续进入驱动TRP1表达的TRP1启动子。

将含有Erg9-KD片段的构建体转化到到大肠杆菌TOP10细胞中。将转化体在2PY(2次植物蛋白胨酵母提取物)、sAMP培养基中生长。将质粒DNA用QIAprep旋转小量制备试剂盒(Qiagen)分离并用SalI-HF(New England Biolabs)消化。为了浓缩，将DNA用乙醇沉淀。将所述片段转化到酿酒酵母中，并将菌落接种在无色氨酸的无机培养基(Verduyn等人,1992.Yeast 8:501-517)琼脂板上。通过诊断PCR和测序证实Erg9-KD构建体的正确整合。进行的Erg9-KD构建体的转化的示意图在图3中示出。所述菌株被命名为STV003。

1.3 UGT2_1a的过表达

对于UGT2_1a的过度表达，使用如共同待决专利申请号W02013/076280和WO2013/144257中所描述的技术。将UGT2a在DNA2.0作为盒(含有同源序列、启动子、基因、终止子、同源序列)订购。关于细节，参见表3。为了获得含有标记和Cre-重组酶的片段，使用如共同待决专利申请号WO2013/135728中所描述的技术。使用赋予对诺尔丝菌素的抗性的NAT标记用于选择。

表3过表达构建体的组成

合适的引物用于扩增。为了扩增整合基因座的5'和3'整合侧翼，使用了来自CEN.PK酵母菌株的合适的引物和基因组DNA。

用表4中列出的片段转化酿酒酵母酵母菌株STV003，并将转化混合物接种在含有50μg/ml诺尔丝菌素(来自Jena Bioscience的Lexy NTC)的YEPhD琼脂板上。

表4用于UGT2a转化的DNA片段

CRE重组酶的表达通过半乳糖的存在活化。为了诱导CRE重组酶的表达，将转化体在YEPh半乳糖培养基上重新划线。这导致位于lox位点之间的标记的外重组。通过诊断PCR证实了UGT2a的正确整合和NAT标记的外重组。所得菌株被命名为STV004。进行的UGT2a构建体的转化的示意图在图4中示出。

1.4至RebA的制备路径：CPS、KS、KO、KAH、CPR、UGT1、UGT3和UGT4的过表达

引起RebA制备的所有路径基因被设计成整合在STV004菌株背景中的一个基因座中。为了扩增用于整合基因座(位点3)的5’和3’整合侧翼，使用了合适的引物和源于CEN.PK酵母菌株的基因组DNA。将不同的基因在DNA2.0定制为盒(包含同源序列、启动子、基因、终止子、同源序列)(参见表5以了解概况)。将源于DNA2.0的DNA溶解至100ng/μl。将该储备溶液进一步稀释至5ng/μl，其中的1μl用于50μl-PCR的混合物中。该反应含有25pmol的各引物。在扩增后，用NucleoSpin 96PCR清除试剂盒(Macherey-Nagel)纯化DNA，或替代地使用乙醇沉淀来浓缩DNA。

表5用于CPS、KS、KO、KAH、CPR、UGT1、UGT3和UGT4的过表达构建体的组成

启动子	开放阅读框	终止子
			Kl prom 12.pro(SEQ ID NO:205)	CPS(SEQ ID NO:61)	Sc Adh2.ter(SEQ ID NO:213)
Sc Pgk1.pro(SEQ ID NO:204)	KS(SEQ ID NO:65)	Sc Tal1.ter(SEQ ID NO:215)
			Sc Eno2.pro(SEQ ID NO:201)	KO(SEQ ID NO:23)	Sc Tpi1.ter(SEQ ID NO:216)
Ag lox_Tef1.pro(SEQ ID NO:206)	KANMX(SEQ ID NO:211)	Ag Tef1_lox.ter(SEQ ID NO:217)
			Sc Tef1.pro(SEQ ID NO:203)	KAH(SEQ ID NO:33)	Sc Gpm1.ter(SEQ ID NO:214)
Kl prom 6.pro(SEQ ID NO:207)	CPR(SEQ ID NO:77)	Sc Pdc1.ter(SEQ ID NO:218)
			Sc Pma1.pro(SEQ ID NO:208)	UGT1(SEQ ID NO:71)	Sc Tdh1.ter(SEQ ID NO:219)
Sc Vps68.pro(SEQ ID NO:209)	UGT3(SEQ ID NO:73)	Sc Adh1.ter(SEQ ID NO:212)
			Sc Oye2.pro(SEQ ID NO:210)	UGT4(SEQ ID NO:75)	Sc Eno1.ter(SEQ ID NO:220)

将到RebA的途径的所有片段(标记和侧翼)(参见表6总述)转化到酿酒酵母菌株STV004中。在20℃下在YEPhD中过夜恢复后，将转化混合物接种在含有200μg/ml G418的YEPhD琼脂上。将这些在30℃下孵育3天。

表6.用于CPS、KS、KO、KanMX、KAH、CPR、UGT1、UGT3和UGT4的转化的DNA片段

片段
	5’INT1
CPS盒
	KS盒
KO盒
	KanMX盒
KAH盒
	CPR盒
UGT1盒
	UGT3盒
UGT4盒
	3’INT1

通过诊断PCR和序列分析(3500基因分析仪,Applied Biosystems)证实了正确的整合。序列反应用BigDye终止子v3.1循环测序试剂盒(Life Technologies)进行。每个反应(10μl)均含有50ng模板和3.2pmol引物。将产物通过乙醇/EDTA沉淀纯化，溶解在10μl HiDi甲酰胺中并施加到装置上。所述菌株被命名为STV016。从GGPP到RebA的途径如何整合到基因组中的示意图在图5中示出。表7列出了本实施例1中使用的菌株。

表7菌株表

1.5STV016的发酵

如上所述构建的酿酒酵母菌株STV016在摇瓶(2升，具有200ml的培养基)中于30℃和220rpm下培养32小时。该培养基是基于Verduyn等人(Verduyn C、Postma E、ScheffersWA、Van Dijken JP.酵母，1992年7月；8(7)：501-517)，其改变了碳和氮源，如在表8中所示。

表8预培养基组成

原料	式	浓度(g/kg)
			半乳糖	C<sub>6</sub>H<sub>12</sub>O<sub>6</sub>	20.0
尿素	(NH<sub>2</sub>)<sub>2</sub>CO	2.3
			磷酸二氢钾	KH<sub>2</sub>PO<sub>4</sub>	3.0
硫酸镁	MgSO<sub>4</sub>.7H<sub>2</sub>O	0.5
			微量元素溶液		1
维生素溶液		1

^a微量元素溶液

^b维生素溶液

组分	式	浓度(g/kg)
			生物素(D-)	C<sub>10</sub>H<sub>16</sub>N<sub>2</sub>O<sub>3</sub>S	0.05
泛酸钙D(+)	C<sub>18</sub>H<sub>32</sub>CaN<sub>2</sub>O<sub>10</sub>	1.00
			烟酸	C<sub>6</sub>H<sub>5</sub>NO<sub>2</sub>	1.00
肌醇	C<sub>6</sub>H<sub>12</sub>O<sub>6</sub>	25.00
			盐酸氯化硫胺素	C<sub>12</sub>H<sub>18</sub>Cl<sub>2</sub>N<sub>4</sub>OS.xH<sub>2</sub>O	1.00
盐酸吡哆醇	C<sub>8</sub>H<sub>12</sub>ClNO<sub>3</sub>	1.00
			对氨基苯甲酸	C<sub>7</sub>H<sub>7</sub>NO<sub>2</sub>	0.20

随后，将摇瓶中200ml内容物转移至发酵罐(起始体积5L)中，其含有在表9中显示的培养基。

表9.组合发酵培养基

原料		最终浓度(g/kg)
			一水葡萄糖	C<sub>6</sub>H<sub>12</sub>O<sub>6</sub>.1H<sub>2</sub>O	4.4
硫酸铵	(NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub>	1
			磷酸二氢钾	KH<sub>2</sub>PO<sub>4</sub>	10
硫酸镁	MgSO<sub>4</sub>.7H<sub>2</sub>O	5
			微量元素溶液	-	8
维生素溶液	-	8

通过添加氨(25wt％)将pH控制在5.0。将温度控制在27℃。通过调整搅拌器速度来将pO₂控制在40％。葡萄糖浓度通过受限于至发酵罐的受控进料保持，如在表10中所显示的。

表10发酵进料培养基的组成

原料	式	最终浓度(g/kg)
			一水葡萄糖	C<sub>6</sub>H<sub>12</sub>O<sub>6</sub>.1H<sub>2</sub>O	550
磷酸二氢钾	KH<sub>2</sub>PO<sub>4</sub>	15.1
			七水硫酸镁	MgSO<sub>4</sub>.7H<sub>2</sub>O	7.5
Verduyn微量元素溶液		12
			Verduyn维生素溶液		12

实施例2：使用LC-MS观察7.1、7.2和7.3

在水/乙醇混合物(菌株STV016)中结晶莱鲍迪甙A之后在母液中用下述的LC-MS系统来观察含有七个葡萄糖分子的甜菊醇糖苷(其还被称为7.1、7.2和7.3)。在纯化之前，通过蒸发来浓缩样品。

在被耦合至配备有以负离子模式操作的电喷雾电离源的XEVO-TQ质谱仪(Waters)的Acquity UPLC(Waters)上分析7.1、7.2和7.3，这是在MRM模式下在用于所研究的所有甜菊醇糖苷的去质子化分子上进行的，在这些m/z 1451.5中，表示含有七个葡萄糖分子的甜菊醇糖苷的去质子化分子。

使用具有作为移动相的(A)在LC-MS级水中的50mM乙酸铵和(B)LC-MS级乙腈的梯度洗脱，以2.1×100mm 1.8μm粒度的Acquity

T3柱实现了色谱分离。4分钟梯度从30％B开始，在0.5分钟内线性增加到35％B且在35％B保持0.8分钟，随后在0.7分钟内线性增加到95％B并在该处保持0.5分钟，然后用30％B进行1.5分钟的再平衡。使用5μl的注射体积将流速保持在0.6ml/min，并将柱温度设置成50℃。为m/z 1451.5观察的各个化合物7.1、7.2和7.3在0.59、0.71和0.74分钟的保留时间上进行洗脱。

对于7.1、7.2和7.3的元素组成的分析而言，用配备有以负离子模式操作的电喷雾电离源的LTQ-轨道阱傅里叶变换质谱仪(Thermo Electron)执行HRMS(高分辨率质谱)分析，这从m/z 300-2000进行扫描。使用Acella LC系统(Thermo Fisher)实现了色谱分离，该系统具有与上述的相同的柱和梯度系统。

使用该色谱系统，各个化合物在0.84、1.20和1.30分钟的保持时间上进行洗脱，其分别如在图6a中所示，且7.1、7.2和7.3被分别在m/z 1451.5786、1451.5793和1451.5793处进行表征，这与1451.5820的理论m/z值具有良好的一致性(分别为-1.8和-2.3ppm)。这些组分的相应化学式为C₆₂H₁₀₀O₃₈，其用于不带电物质。

实施例3：使用制备型LC-UV进行的7.1、7.2和7.3的纯化

从含有最少量的感兴趣的化合物的酵母菌属培养液(菌株STV016)的乙醇提取物执行7.1、7.2和7.3的纯化。使用反相色谱(Waters Atlantis T3，30*150mm，5μm)进行制备分离，用LC-MS级水和乙腈作为洗脱液来进行梯度洗脱。使用40ml/min的流速和300μl的注射体积。

执行大约100次的注射，并且通过在210nm处的UV检测来触发感兴趣的化合物。在LC-MS和NMR分析之前，将7.1、7.2和7.3的所有级分合并且冷冻干燥。

使用LC-MS的在制备纯化后进行的用于质量确认和纯度测定的7.1、7.2和7.3的 LC-MS

在被耦合至配备有以负离子模式操作的电喷雾电离源的XEVO-TQ质谱仪(Waters)的Acquity UPLC(Waters)上分析7.1、7.2和7.3的纯度，这是在MRM模式下在用于所研究的所有甜菊醇糖苷的去质子化分子上进行的，在这些m/z 1451.5中，表示含有七个葡萄糖分子的甜菊醇糖苷的去质子化分子。在0.59分钟的保留时间处进行洗脱的7.1可被估计为超过80％纯，而在0.71和0.74分钟的保留时间处进行洗脱的7.2和7.3可被估计为超过90％纯且7.3仍含有约5％的7.2，如在图6b中所示。

用配备有以负离子模式操作的电喷雾电离源的LTQ-轨道阱傅里叶变换质谱仪(Thermo Electron)执行HRMS(高分辨率质谱)分析，检查各个化合物的元素组成且发现其与用于不带电物质的化学式C₆₂H₁₀₀O₃₈相对应的理论质量具有良好的一致性。

实施例4：莱鲍迪甙7.1的分析

将如在实施例3中所述获得的1.1mg的级分7.1溶解于1.3ml的CDCl₃/吡啶-d5 1/3(w/w)和2滴DCOOD中。

一系列具有小混合时间增量的COZY和TOCSY 2D NMR谱为所有三种莱鲍迪甙以及对映贝壳杉烷二萜核心提供(七个糖单位的)每个旋转系统的几乎所有质子的分配。HSQC实验允许分配相应的C-H耦合。

基于其在HMBC中与对映贝壳杉烷二萜核心的质子的长程相关性来识别glc^I和glc^II的端基异构H。

在相应的ROESY谱中观察到的H2^I-H1^V和H3^I-H1^VI以及H2^II-H1^III和H3^II-H1^IIII的长程相关性允许进行glc^I和glc^II的取代位点的分配。还通过在HMBC实验中glc^III至glc^VI的端基异构质子与glc^I和glc^II的¹³C原子，即H1^III-C2^II、H1^IIII-C3^II、H1^V-C2^I和H1^VI-C3^I的长程相关性来证实该分配。糖glc^III、glc^IV、glc^V和glc^VI的位置与在莱鲍迪甙M的结构中的相同(图10)。

端基异构H1^VII(5.86ppm对4.5至4.6ppm)和小耦合常数(3.8Hz对7.8Hz)的低场位移指示第七个糖残基具有α构型。

莱鲍迪甙7.1中第七个糖的位置可以根据在ROESY实验中的H1^VII和C3^III的长程HMBC耦合，H1^VII-H3^III的长程质子耦合以及C3^III的低场位移(与约78-79ppm的未取代C3原子相比，其为83.8ppm)来进行识别。在图7中描绘了莱鲍迪甙7.1的结构。用于莱鲍迪甙7.1的所有¹H和¹³C NMR化学位移均列于表11中。为了进行比较，莱鲍迪甙M的数据也包括在内。

表11在320K记录的在CDCl₃/吡啶1/3和3滴DCOOD中的莱鲍迪甙7.1以及在300K， δ_TMS＝0记录的在CDCl₃/吡啶1/1和3滴DCOOD中的莱鲍迪甙M的¹H和¹³C NMR化学位移

实施例5：莱鲍迪甙7.2的分析

将2.5mg的样品溶解于1ml的CDCl₃/吡啶-d5 1/1(w/w)和2滴DCOOD中。

一系列具有小混合时间增量的COSY和TOCSY 2D NMR为所有三种莱鲍迪甙以及对映贝壳杉烷二萜核心提供(七个糖单位的)每个旋转系统的几乎所有质子的分配。HSQC实验允许分配相应的C-H耦合。

基于其在HMBC中与对映贝壳杉烷二萜核心的质子的长程相关性来识别glc^I和glc^II的端基异构H。

糖glc^III、glc^IV、glc^V和glc^VI的位置与在莱鲍迪甙M的结构中的相同，且在专门用于莱鲍迪甙7.1的结构的分配的部分中更详细地描述了该分配。

莱鲍迪甙7.2中第七个糖的位置可以根据在ROESY实验中的H6^IV和C1^VII的长程HMBC耦合，H1^VII-H6^IV的长程质子耦合以及C6^IV的低场位移(与62-64ppm的剩余的C6原子相比，其为69.4ppm)来进行识别。第七个糖经β-糖苷键被附接至Glc^IV。在图8中描绘了莱鲍迪甙7.2的结构。莱鲍迪甙7.2的所有¹H和¹³C NMR化学位移均列于表12中。为了进行比较，莱鲍迪甙M的数据也包括在内。

表12在300K，δ_TMS＝0记录的在CDCl₃/吡啶1/1和2滴DCOOD中的莱鲍迪甙7.2以及在 CDCl₃/吡啶1/1和3滴DCOOD中的莱鲍迪甙M的¹H和¹³C NMR化学位移

实施例6：莱鲍迪甙7.3的分析

将2.3mg的样品溶解于1ml的CDCl₃/吡啶-d5 1/2(w/w)和3滴DCOOD中。

一系列具有小混合时间增量的COSY和TOCSY 2D NMR为所有三种莱鲍迪甙以及对映贝壳杉烷二萜核心提供(七个糖单位的)每个旋转系统的几乎所有质子的分配。HSQC实验允许分配相应的C-H耦合。

基于其在HMBC中与对映贝壳杉烷二萜核心的质子的长程相关性来识别glc^I和glc^II的端基异构H。

糖glc^III、glc^IV、glc^V和glc^VI的位置与在莱鲍迪甙M的结构中的相同，且在专门用于莱鲍迪甙7.1的结构的分配的部分中更详细地描述了该分配。

莱鲍迪甙7.3中第七个糖的位置可以根据在ROESY实验中的H1^VII和C6^VI的长程HMBC耦合，H1^VII-H6^VI的长程质子耦合以及C6^VI的低场位移(与61-63ppm的剩余的C6原子相比，其为69.5ppm)来进行识别。第七个糖经β-糖苷键被附接至Glc^VI。在图9中描绘了莱鲍迪甙7.3的结构。莱鲍迪甙7.3的所有¹H和¹³C NMR化学位移均列于表13中。为了进行比较，莱鲍迪甙M的数据也包括在内。

表13在300K，δ_TMS＝0记录的在CDCl₃/吡啶1/2和3滴DCOOD中的莱鲍迪甙7.3以及在 CDCl₃/吡啶1/1和3滴DCOOD中的莱鲍迪甙M的¹H和¹³C NMR化学位移

总之，如在表14中所显示的，确定了三种新的莱鲍迪甙。

表14新的莱鲍迪甙的概况

一般物料和方法(NMR分析)

为莱鲍迪甙样品中的每一个优化溶剂混合物以获得端基异构质子的信号的最佳可能分辨率。样品的量和溶剂量对于峰的分辨率来说是至关重要的，这是因为峰，特别是端基异构的峰的位移是依赖于浓度和pH的(图12)。

在300K记录莱鲍迪甙7.2和7.3的谱，而在莱鲍迪甙7.1的情况下，则必须使用更高的温度。在300K时，在莱鲍迪甙7.1谱中的共振相当得宽，这指示溶解性差或构象过程缓慢。因此，在320K的样品温度下实现了所有信号的最终分配。

对于每个样品而言，进行了各种2D NMR实验：在Bruker Avance III600和700MHz谱仪上于320K下记录COSY、TOCSY(具有40、50、60、70、80、90和100ms的混合时间)、HSQC、HMBC和ROESY(225、400ms的混合时间)的谱。在样品部分中指定了用于每个样品的详细分配。

在实施例4、5和6中，分别在图11a和图11b中显示出甜菊醇和葡萄糖的原子编号。

表15：序列表的描述

截去变灰的标识符且因此为所提及的UniProt标识符的片段。

序列表

<110> 帝斯曼知识产权资产管理有限公司

<120> 甜菊醇糖苷

<130> 30983-WO-PCT

<150> US62/142631

<151> 2015-04-03

<160> 223

<170> PatentIn version 3.5

<210> 1

<211> 2397

<212> DNA

<213> Lactuca sativa

<220>

<221> CDS

<222> (1)..(2397)

<400> 1

atg aaa acc atg att tct tct cca atc cca gct ttc cac cca aga ttt 48

Met Lys Thr Met Ile Ser Ser Pro Ile Pro Ala Phe His Pro Arg Phe

1 5 10 15

tct cca gct gct ggt tcc aga aga tta tct cca atc ttg cca tct tcc 96

Ser Pro Ala Ala Gly Ser Arg Arg Leu Ser Pro Ile Leu Pro Ser Ser

20 25 30

ggt tct gtt gtc ttg act ggt tcc aag act caa tgt aag gcc gtt tct 144

Gly Ser Val Val Leu Thr Gly Ser Lys Thr Gln Cys Lys Ala Val Ser

35 40 45

aaa tct cca act caa gaa tac ttt gat gtt ttg caa aag aac ggt ttg 192

Lys Ser Pro Thr Gln Glu Tyr Phe Asp Val Leu Gln Lys Asn Gly Leu

50 55 60

cca ttc atc aac tgg caa aac gat gtc gtt gaa gat gaa ttg gac aag 240

Pro Phe Ile Asn Trp Gln Asn Asp Val Val Glu Asp Glu Leu Asp Lys

65 70 75 80

gaa aag aag atc ttg tac cca aac gac gaa atc aag ggt ttc gtt gaa 288

Glu Lys Lys Ile Leu Tyr Pro Asn Asp Glu Ile Lys Gly Phe Val Glu

85 90 95

aga atc aaa gtt atg tta ggt tcc atg gac gaa ggt gaa atc act gtc 336

Arg Ile Lys Val Met Leu Gly Ser Met Asp Glu Gly Glu Ile Thr Val

100 105 110

tct gct tat gac acc gct tgg gtt gct ttg gtc caa gat atc gat ggt 384

Ser Ala Tyr Asp Thr Ala Trp Val Ala Leu Val Gln Asp Ile Asp Gly

115 120 125

aac ggt aga cca gaa ttt cct tct tct cta gaa tgg atc gtc aag aac 432

Asn Gly Arg Pro Glu Phe Pro Ser Ser Leu Glu Trp Ile Val Lys Asn

130 135 140

caa tta tcc gat ggt tcc tgg ggt gac cat ttg atc ttc tct gct cac 480

Gln Leu Ser Asp Gly Ser Trp Gly Asp His Leu Ile Phe Ser Ala His

145 150 155 160

gac aga atc att aac act ttg gct tgt gtc att gct ttg act tcc tgg 528

Asp Arg Ile Ile Asn Thr Leu Ala Cys Val Ile Ala Leu Thr Ser Trp

165 170 175

aac gtt cat cca ggt aag tgt caa aag ggt tta aag ttc ttg aac gac 576

Asn Val His Pro Gly Lys Cys Gln Lys Gly Leu Lys Phe Leu Asn Asp

180 185 190

aac atc tcc aag ttg gaa gaa gaa aac cca gaa cac atg cca atc ggt 624

Asn Ile Ser Lys Leu Glu Glu Glu Asn Pro Glu His Met Pro Ile Gly

195 200 205

ttt gaa gtt gct ttc cca tct ttg att gat att gcc aga aag ttg gat 672

Phe Glu Val Ala Phe Pro Ser Leu Ile Asp Ile Ala Arg Lys Leu Asp

210 215 220

atc caa gtt cca gaa gat tcc cca gct ttg aag gaa att tac gcc aga 720

Ile Gln Val Pro Glu Asp Ser Pro Ala Leu Lys Glu Ile Tyr Ala Arg

225 230 235 240

aga aac ttg aaa ttg acc aag att cca aag tct ttg atg cac aag gtt 768

Arg Asn Leu Lys Leu Thr Lys Ile Pro Lys Ser Leu Met His Lys Val

245 250 255

cca act act ttg ttg cat tct ttg gaa ggt atg cca gac ttg gaa tgg 816

Pro Thr Thr Leu Leu His Ser Leu Glu Gly Met Pro Asp Leu Glu Trp

260 265 270

gaa aag ttg ttg aag cta caa tgt aag gat ggt tct ttc ttg ttc tct 864

Glu Lys Leu Leu Lys Leu Gln Cys Lys Asp Gly Ser Phe Leu Phe Ser

275 280 285

cca tct tcc act gct ttc gct ttg atg caa acc aag gac caa aag tgt 912

Pro Ser Ser Thr Ala Phe Ala Leu Met Gln Thr Lys Asp Gln Lys Cys

290 295 300

ttg caa tac ttg act gac gct gtc acc aag ttc aac ggt ggt gtt cca 960

Leu Gln Tyr Leu Thr Asp Ala Val Thr Lys Phe Asn Gly Gly Val Pro

305 310 315 320

aac gtc tac cca gtc gac ttg ttc gaa cac atc tgg gtt gtt gac aga 1008

Asn Val Tyr Pro Val Asp Leu Phe Glu His Ile Trp Val Val Asp Arg

325 330 335

ttg caa aga tta ggt atc tcc aga tac ttc gac tct gaa atc aaa gac 1056

Leu Gln Arg Leu Gly Ile Ser Arg Tyr Phe Asp Ser Glu Ile Lys Asp

340 345 350

tgt gtc gac tac atc tac aga tac tgg acc aag gat ggt atc tgt tgg 1104

Cys Val Asp Tyr Ile Tyr Arg Tyr Trp Thr Lys Asp Gly Ile Cys Trp

355 360 365

gct aag aac tcc aac gtt caa gat att gat gac acc gct atg ggt ttc 1152

Ala Lys Asn Ser Asn Val Gln Asp Ile Asp Asp Thr Ala Met Gly Phe

370 375 380

aga gtt ttg aga atg cac ggt tac aag gtc act act gat gtt ttc cgt 1200

Arg Val Leu Arg Met His Gly Tyr Lys Val Thr Thr Asp Val Phe Arg

385 390 395 400

caa ttc gaa aag gac ggt aaa ttc gtt tgt ttc cca ggt caa acc acc 1248

Gln Phe Glu Lys Asp Gly Lys Phe Val Cys Phe Pro Gly Gln Thr Thr

405 410 415

caa gct gtc acc ggt atg ttc aac ttg ttt aga gct tct caa gtc ttg 1296

Gln Ala Val Thr Gly Met Phe Asn Leu Phe Arg Ala Ser Gln Val Leu

420 425 430

ttc cca gac gaa aag att ttg gaa gat gct aag aaa ttc tct tac aac 1344

Phe Pro Asp Glu Lys Ile Leu Glu Asp Ala Lys Lys Phe Ser Tyr Asn

435 440 445

tac ttg aag gaa aag caa tcc acc aac gaa tta ttg gac aaa tgg atc 1392

Tyr Leu Lys Glu Lys Gln Ser Thr Asn Glu Leu Leu Asp Lys Trp Ile

450 455 460

att gcc aag gac ttg cca ggt gaa gtt gaa tac gct tta gat gtt cca 1440

Ile Ala Lys Asp Leu Pro Gly Glu Val Glu Tyr Ala Leu Asp Val Pro

465 470 475 480

tgg tac gct tcc ttg cca cgt ttg gaa acc aga ttc tac ttg gaa caa 1488

Trp Tyr Ala Ser Leu Pro Arg Leu Glu Thr Arg Phe Tyr Leu Glu Gln

485 490 495

tac ggt ggt gaa gat gac gtc tgg atc ggt aag act ttg tac aga atg 1536

Tyr Gly Gly Glu Asp Asp Val Trp Ile Gly Lys Thr Leu Tyr Arg Met

500 505 510

ggt aat gtc tcc aac aac acc tac ttg gaa atg gcc aaa ttg gac tac 1584

Gly Asn Val Ser Asn Asn Thr Tyr Leu Glu Met Ala Lys Leu Asp Tyr

515 520 525

aac aac tgt ttg gcc att cat cac ttg gaa tgg aac acc atg caa caa 1632

Asn Asn Cys Leu Ala Ile His His Leu Glu Trp Asn Thr Met Gln Gln

530 535 540

tgg tac gtt gac ttc ggt atg gaa aga ttc ggt act tct gat atc act 1680

Trp Tyr Val Asp Phe Gly Met Glu Arg Phe Gly Thr Ser Asp Ile Thr

545 550 555 560

tct cta tta gtc tct tac tac ttg gct gct gcc tcc gtc ttt gaa cct 1728

Ser Leu Leu Val Ser Tyr Tyr Leu Ala Ala Ala Ser Val Phe Glu Pro

565 570 575

gaa cgt tct aag gaa aga att gct tgg gct aag acc acc act ttg gtt 1776

Glu Arg Ser Lys Glu Arg Ile Ala Trp Ala Lys Thr Thr Thr Leu Val

580 585 590

gac acc atc tct tct ttc ttc cac tcc ttg aag atc tct aac gaa cac 1824

Asp Thr Ile Ser Ser Phe Phe His Ser Leu Lys Ile Ser Asn Glu His

595 600 605

cgt cgt gaa ttc gtt gaa gaa ttc aga aac atc tcc aac tcc atc cac 1872

Arg Arg Glu Phe Val Glu Glu Phe Arg Asn Ile Ser Asn Ser Ile His

610 615 620

cac gct aag tac ggt aag cca tgg cac ggt ttg atg gtt gct ttg aaa 1920

His Ala Lys Tyr Gly Lys Pro Trp His Gly Leu Met Val Ala Leu Lys

625 630 635 640

ggt act tta cac gaa att gcc ttg gat gtc ttg atg act cac aga aga 1968

Gly Thr Leu His Glu Ile Ala Leu Asp Val Leu Met Thr His Arg Arg

645 650 655

gac att cac cct caa ttg cac cat gct tgg gaa atg tgg ttg atg aga 2016

Asp Ile His Pro Gln Leu His His Ala Trp Glu Met Trp Leu Met Arg

660 665 670

tgg caa caa ggt gtc gat gcc act gaa ggt caa gct gaa tta att gtt 2064

Trp Gln Gln Gly Val Asp Ala Thr Glu Gly Gln Ala Glu Leu Ile Val

675 680 685

caa acc atc aac atg act gct ggt aga tgg gtt tcc aat gaa tta ttg 2112

Gln Thr Ile Asn Met Thr Ala Gly Arg Trp Val Ser Asn Glu Leu Leu

690 695 700

gct cac cca caa tac aga tta ttg tcc tct gtc atc aac aat atc tgt 2160

Ala His Pro Gln Tyr Arg Leu Leu Ser Ser Val Ile Asn Asn Ile Cys

705 710 715 720

cac gaa atc tac cac aac aga acc tgt atg gaa gtc aac tct acc acc 2208

His Glu Ile Tyr His Asn Arg Thr Cys Met Glu Val Asn Ser Thr Thr

725 730 735

att tcc act tct att gac tcc aag atg caa gaa ttg gtt caa tta gtc 2256

Ile Ser Thr Ser Ile Asp Ser Lys Met Gln Glu Leu Val Gln Leu Val

740 745 750

ttg tct gac tct ttg gac gac ttg gac caa gat ttg aag caa acc ttc 2304

Leu Ser Asp Ser Leu Asp Asp Leu Asp Gln Asp Leu Lys Gln Thr Phe

755 760 765

ttg act gtt gcc aag act ttc tac tac aag gct tac tgt gac cca gaa 2352

Leu Thr Val Ala Lys Thr Phe Tyr Tyr Lys Ala Tyr Cys Asp Pro Glu

770 775 780

acc atc aac gtt cac att tct aag gtc atg ttc gaa acc att att 2397

Thr Ile Asn Val His Ile Ser Lys Val Met Phe Glu Thr Ile Ile

785 790 795

<210> 2

<211> 799

<212> PRT

<213> Lactuca sativa

<400> 2

Met Lys Thr Met Ile Ser Ser Pro Ile Pro Ala Phe His Pro Arg Phe

1 5 10 15

Ser Pro Ala Ala Gly Ser Arg Arg Leu Ser Pro Ile Leu Pro Ser Ser

20 25 30

Gly Ser Val Val Leu Thr Gly Ser Lys Thr Gln Cys Lys Ala Val Ser

35 40 45

Lys Ser Pro Thr Gln Glu Tyr Phe Asp Val Leu Gln Lys Asn Gly Leu

50 55 60

Pro Phe Ile Asn Trp Gln Asn Asp Val Val Glu Asp Glu Leu Asp Lys

65 70 75 80

Glu Lys Lys Ile Leu Tyr Pro Asn Asp Glu Ile Lys Gly Phe Val Glu

85 90 95

Arg Ile Lys Val Met Leu Gly Ser Met Asp Glu Gly Glu Ile Thr Val

100 105 110

Ser Ala Tyr Asp Thr Ala Trp Val Ala Leu Val Gln Asp Ile Asp Gly

115 120 125

Asn Gly Arg Pro Glu Phe Pro Ser Ser Leu Glu Trp Ile Val Lys Asn

130 135 140

Gln Leu Ser Asp Gly Ser Trp Gly Asp His Leu Ile Phe Ser Ala His

145 150 155 160

Asp Arg Ile Ile Asn Thr Leu Ala Cys Val Ile Ala Leu Thr Ser Trp

165 170 175

Asn Val His Pro Gly Lys Cys Gln Lys Gly Leu Lys Phe Leu Asn Asp

180 185 190

Asn Ile Ser Lys Leu Glu Glu Glu Asn Pro Glu His Met Pro Ile Gly

195 200 205

Phe Glu Val Ala Phe Pro Ser Leu Ile Asp Ile Ala Arg Lys Leu Asp

210 215 220

Ile Gln Val Pro Glu Asp Ser Pro Ala Leu Lys Glu Ile Tyr Ala Arg

225 230 235 240

Arg Asn Leu Lys Leu Thr Lys Ile Pro Lys Ser Leu Met His Lys Val

245 250 255

Pro Thr Thr Leu Leu His Ser Leu Glu Gly Met Pro Asp Leu Glu Trp

260 265 270

Glu Lys Leu Leu Lys Leu Gln Cys Lys Asp Gly Ser Phe Leu Phe Ser

275 280 285

Pro Ser Ser Thr Ala Phe Ala Leu Met Gln Thr Lys Asp Gln Lys Cys

290 295 300

Leu Gln Tyr Leu Thr Asp Ala Val Thr Lys Phe Asn Gly Gly Val Pro

305 310 315 320

Asn Val Tyr Pro Val Asp Leu Phe Glu His Ile Trp Val Val Asp Arg

325 330 335

Leu Gln Arg Leu Gly Ile Ser Arg Tyr Phe Asp Ser Glu Ile Lys Asp

340 345 350

Cys Val Asp Tyr Ile Tyr Arg Tyr Trp Thr Lys Asp Gly Ile Cys Trp

355 360 365

Ala Lys Asn Ser Asn Val Gln Asp Ile Asp Asp Thr Ala Met Gly Phe

370 375 380

Arg Val Leu Arg Met His Gly Tyr Lys Val Thr Thr Asp Val Phe Arg

385 390 395 400

Gln Phe Glu Lys Asp Gly Lys Phe Val Cys Phe Pro Gly Gln Thr Thr

405 410 415

Gln Ala Val Thr Gly Met Phe Asn Leu Phe Arg Ala Ser Gln Val Leu

420 425 430

Phe Pro Asp Glu Lys Ile Leu Glu Asp Ala Lys Lys Phe Ser Tyr Asn

435 440 445

Tyr Leu Lys Glu Lys Gln Ser Thr Asn Glu Leu Leu Asp Lys Trp Ile

450 455 460

Ile Ala Lys Asp Leu Pro Gly Glu Val Glu Tyr Ala Leu Asp Val Pro

465 470 475 480

Trp Tyr Ala Ser Leu Pro Arg Leu Glu Thr Arg Phe Tyr Leu Glu Gln

485 490 495

Tyr Gly Gly Glu Asp Asp Val Trp Ile Gly Lys Thr Leu Tyr Arg Met

500 505 510

Gly Asn Val Ser Asn Asn Thr Tyr Leu Glu Met Ala Lys Leu Asp Tyr

515 520 525

Asn Asn Cys Leu Ala Ile His His Leu Glu Trp Asn Thr Met Gln Gln

530 535 540

Trp Tyr Val Asp Phe Gly Met Glu Arg Phe Gly Thr Ser Asp Ile Thr

545 550 555 560

Ser Leu Leu Val Ser Tyr Tyr Leu Ala Ala Ala Ser Val Phe Glu Pro

565 570 575

Glu Arg Ser Lys Glu Arg Ile Ala Trp Ala Lys Thr Thr Thr Leu Val

580 585 590

Asp Thr Ile Ser Ser Phe Phe His Ser Leu Lys Ile Ser Asn Glu His

595 600 605

Arg Arg Glu Phe Val Glu Glu Phe Arg Asn Ile Ser Asn Ser Ile His

610 615 620

His Ala Lys Tyr Gly Lys Pro Trp His Gly Leu Met Val Ala Leu Lys

625 630 635 640

Gly Thr Leu His Glu Ile Ala Leu Asp Val Leu Met Thr His Arg Arg

645 650 655

Asp Ile His Pro Gln Leu His His Ala Trp Glu Met Trp Leu Met Arg

660 665 670

Trp Gln Gln Gly Val Asp Ala Thr Glu Gly Gln Ala Glu Leu Ile Val

675 680 685

Gln Thr Ile Asn Met Thr Ala Gly Arg Trp Val Ser Asn Glu Leu Leu

690 695 700

Ala His Pro Gln Tyr Arg Leu Leu Ser Ser Val Ile Asn Asn Ile Cys

705 710 715 720

His Glu Ile Tyr His Asn Arg Thr Cys Met Glu Val Asn Ser Thr Thr

725 730 735

Ile Ser Thr Ser Ile Asp Ser Lys Met Gln Glu Leu Val Gln Leu Val

740 745 750

Leu Ser Asp Ser Leu Asp Asp Leu Asp Gln Asp Leu Lys Gln Thr Phe

755 760 765

Leu Thr Val Ala Lys Thr Phe Tyr Tyr Lys Ala Tyr Cys Asp Pro Glu

770 775 780

Thr Ile Asn Val His Ile Ser Lys Val Met Phe Glu Thr Ile Ile

785 790 795

<210> 3

<211> 2271

<212> DNA

<213> Lactuca sativa

<220>

<221> CDS

<222> (1)..(2271)

<400> 3

atg tgt aag gcc gtt tct aaa tct cca act caa gaa tac ttt gat gtt 48

Met Cys Lys Ala Val Ser Lys Ser Pro Thr Gln Glu Tyr Phe Asp Val

1 5 10 15

ttg caa aag aac ggt ttg cca ttc atc aac tgg caa aac gat gtc gtt 96

Leu Gln Lys Asn Gly Leu Pro Phe Ile Asn Trp Gln Asn Asp Val Val

20 25 30

gaa gat gaa ttg gac aag gaa aag aag atc ttg tac cca aac gac gaa 144

Glu Asp Glu Leu Asp Lys Glu Lys Lys Ile Leu Tyr Pro Asn Asp Glu

35 40 45

atc aag ggt ttc gtt gaa aga atc aaa gtt atg tta ggt tcc atg gac 192

Ile Lys Gly Phe Val Glu Arg Ile Lys Val Met Leu Gly Ser Met Asp

50 55 60

gaa ggt gaa atc act gtc tct gct tat gac acc gct tgg gtt gct ttg 240

Glu Gly Glu Ile Thr Val Ser Ala Tyr Asp Thr Ala Trp Val Ala Leu

65 70 75 80

gtc caa gat atc gat ggt aac ggt aga cca gaa ttt cct tct tct cta 288

Val Gln Asp Ile Asp Gly Asn Gly Arg Pro Glu Phe Pro Ser Ser Leu

85 90 95

gaa tgg atc gtc aag aac caa tta tcc gat ggt tcc tgg ggt gac cat 336

Glu Trp Ile Val Lys Asn Gln Leu Ser Asp Gly Ser Trp Gly Asp His

100 105 110

ttg atc ttc tct gct cac gac aga atc att aac act ttg gct tgt gtc 384

Leu Ile Phe Ser Ala His Asp Arg Ile Ile Asn Thr Leu Ala Cys Val

115 120 125

att gct ttg act tcc tgg aac gtt cat cca ggt aag tgt caa aag ggt 432

Ile Ala Leu Thr Ser Trp Asn Val His Pro Gly Lys Cys Gln Lys Gly

130 135 140

tta aag ttc ttg aac gac aac atc tcc aag ttg gaa gaa gaa aac cca 480

Leu Lys Phe Leu Asn Asp Asn Ile Ser Lys Leu Glu Glu Glu Asn Pro

145 150 155 160

gaa cac atg cca atc ggt ttt gaa gtt gct ttc cca tct ttg att gat 528

Glu His Met Pro Ile Gly Phe Glu Val Ala Phe Pro Ser Leu Ile Asp

165 170 175

att gcc aga aag ttg gat atc caa gtt cca gaa gat tcc cca gct ttg 576

Ile Ala Arg Lys Leu Asp Ile Gln Val Pro Glu Asp Ser Pro Ala Leu

180 185 190

aag gaa att tac gcc aga aga aac ttg aaa ttg acc aag att cca aag 624

Lys Glu Ile Tyr Ala Arg Arg Asn Leu Lys Leu Thr Lys Ile Pro Lys

195 200 205

tct ttg atg cac aag gtt cca act act ttg ttg cat tct ttg gaa ggt 672

Ser Leu Met His Lys Val Pro Thr Thr Leu Leu His Ser Leu Glu Gly

210 215 220

atg cca gac ttg gaa tgg gaa aag ttg ttg aag cta caa tgt aag gat 720

Met Pro Asp Leu Glu Trp Glu Lys Leu Leu Lys Leu Gln Cys Lys Asp

225 230 235 240

ggt tct ttc ttg ttc tct cca tct tcc act gct ttc gct ttg atg caa 768

Gly Ser Phe Leu Phe Ser Pro Ser Ser Thr Ala Phe Ala Leu Met Gln

245 250 255

acc aag gac caa aag tgt ttg caa tac ttg act gac gct gtc acc aag 816

Thr Lys Asp Gln Lys Cys Leu Gln Tyr Leu Thr Asp Ala Val Thr Lys

260 265 270

ttc aac ggt ggt gtt cca aac gtc tac cca gtc gac ttg ttc gaa cac 864

Phe Asn Gly Gly Val Pro Asn Val Tyr Pro Val Asp Leu Phe Glu His

275 280 285

atc tgg gtt gtt gac aga ttg caa aga tta ggt atc tcc aga tac ttc 912

Ile Trp Val Val Asp Arg Leu Gln Arg Leu Gly Ile Ser Arg Tyr Phe

290 295 300

gac tct gaa atc aaa gac tgt gtc gac tac atc tac aga tac tgg acc 960

Asp Ser Glu Ile Lys Asp Cys Val Asp Tyr Ile Tyr Arg Tyr Trp Thr

305 310 315 320

aag gat ggt atc tgt tgg gct aag aac tcc aac gtt caa gat att gat 1008

Lys Asp Gly Ile Cys Trp Ala Lys Asn Ser Asn Val Gln Asp Ile Asp

325 330 335

gac acc gct atg ggt ttc aga gtt ttg aga atg cac ggt tac aag gtc 1056

Asp Thr Ala Met Gly Phe Arg Val Leu Arg Met His Gly Tyr Lys Val

340 345 350

act act gat gtt ttc cgt caa ttc gaa aag gac ggt aaa ttc gtt tgt 1104

Thr Thr Asp Val Phe Arg Gln Phe Glu Lys Asp Gly Lys Phe Val Cys

355 360 365

ttc cca ggt caa acc acc caa gct gtc acc ggt atg ttc aac ttg ttt 1152

Phe Pro Gly Gln Thr Thr Gln Ala Val Thr Gly Met Phe Asn Leu Phe

370 375 380

aga gct tct caa gtc ttg ttc cca gac gaa aag att ttg gaa gat gct 1200

Arg Ala Ser Gln Val Leu Phe Pro Asp Glu Lys Ile Leu Glu Asp Ala

385 390 395 400

aag aaa ttc tct tac aac tac ttg aag gaa aag caa tcc acc aac gaa 1248

Lys Lys Phe Ser Tyr Asn Tyr Leu Lys Glu Lys Gln Ser Thr Asn Glu

405 410 415

tta ttg gac aaa tgg atc att gcc aag gac ttg cca ggt gaa gtt gaa 1296

Leu Leu Asp Lys Trp Ile Ile Ala Lys Asp Leu Pro Gly Glu Val Glu

420 425 430

tac gct tta gat gtt cca tgg tac gct tcc ttg cca cgt ttg gaa acc 1344

Tyr Ala Leu Asp Val Pro Trp Tyr Ala Ser Leu Pro Arg Leu Glu Thr

435 440 445

aga ttc tac ttg gaa caa tac ggt ggt gaa gat gac gtc tgg atc ggt 1392

Arg Phe Tyr Leu Glu Gln Tyr Gly Gly Glu Asp Asp Val Trp Ile Gly

450 455 460

aag act ttg tac aga atg ggt aat gtc tcc aac aac acc tac ttg gaa 1440

Lys Thr Leu Tyr Arg Met Gly Asn Val Ser Asn Asn Thr Tyr Leu Glu

465 470 475 480

atg gcc aaa ttg gac tac aac aac tgt ttg gcc att cat cac ttg gaa 1488

Met Ala Lys Leu Asp Tyr Asn Asn Cys Leu Ala Ile His His Leu Glu

485 490 495

tgg aac acc atg caa caa tgg tac gtt gac ttc ggt atg gaa aga ttc 1536

Trp Asn Thr Met Gln Gln Trp Tyr Val Asp Phe Gly Met Glu Arg Phe

500 505 510

ggt act tct gat atc act tct cta tta gtc tct tac tac ttg gct gct 1584

Gly Thr Ser Asp Ile Thr Ser Leu Leu Val Ser Tyr Tyr Leu Ala Ala

515 520 525

gcc tcc gtc ttt gaa cct gaa cgt tct aag gaa aga att gct tgg gct 1632

Ala Ser Val Phe Glu Pro Glu Arg Ser Lys Glu Arg Ile Ala Trp Ala

530 535 540

aag acc acc act ttg gtt gac acc atc tct tct ttc ttc cac tcc ttg 1680

Lys Thr Thr Thr Leu Val Asp Thr Ile Ser Ser Phe Phe His Ser Leu

545 550 555 560

aag atc tct aac gaa cac cgt cgt gaa ttc gtt gaa gaa ttc aga aac 1728

Lys Ile Ser Asn Glu His Arg Arg Glu Phe Val Glu Glu Phe Arg Asn

565 570 575

atc tcc aac tcc atc cac cac gct aag tac ggt aag cca tgg cac ggt 1776

Ile Ser Asn Ser Ile His His Ala Lys Tyr Gly Lys Pro Trp His Gly

580 585 590

ttg atg gtt gct ttg aaa ggt act tta cac gaa att gcc ttg gat gtc 1824

Leu Met Val Ala Leu Lys Gly Thr Leu His Glu Ile Ala Leu Asp Val

595 600 605

ttg atg act cac aga aga gac att cac cct caa ttg cac cat gct tgg 1872

Leu Met Thr His Arg Arg Asp Ile His Pro Gln Leu His His Ala Trp

610 615 620

gaa atg tgg ttg atg aga tgg caa caa ggt gtc gat gcc act gaa ggt 1920

Glu Met Trp Leu Met Arg Trp Gln Gln Gly Val Asp Ala Thr Glu Gly

625 630 635 640

caa gct gaa tta att gtt caa acc atc aac atg act gct ggt aga tgg 1968

Gln Ala Glu Leu Ile Val Gln Thr Ile Asn Met Thr Ala Gly Arg Trp

645 650 655

gtt tcc aat gaa tta ttg gct cac cca caa tac aga tta ttg tcc tct 2016

Val Ser Asn Glu Leu Leu Ala His Pro Gln Tyr Arg Leu Leu Ser Ser

660 665 670

gtc atc aac aat atc tgt cac gaa atc tac cac aac aga acc tgt atg 2064

Val Ile Asn Asn Ile Cys His Glu Ile Tyr His Asn Arg Thr Cys Met

675 680 685

gaa gtc aac tct acc acc att tcc act tct att gac tcc aag atg caa 2112

Glu Val Asn Ser Thr Thr Ile Ser Thr Ser Ile Asp Ser Lys Met Gln

690 695 700

gaa ttg gtt caa tta gtc ttg tct gac tct ttg gac gac ttg gac caa 2160

Glu Leu Val Gln Leu Val Leu Ser Asp Ser Leu Asp Asp Leu Asp Gln

705 710 715 720

gat ttg aag caa acc ttc ttg act gtt gcc aag act ttc tac tac aag 2208

Asp Leu Lys Gln Thr Phe Leu Thr Val Ala Lys Thr Phe Tyr Tyr Lys

725 730 735

gct tac tgt gac cca gaa acc atc aac gtt cac att tct aag gtc atg 2256

Ala Tyr Cys Asp Pro Glu Thr Ile Asn Val His Ile Ser Lys Val Met

740 745 750

ttc gaa acc att att 2271

Phe Glu Thr Ile Ile

755

<210> 4

<211> 757

<212> PRT

<213> Lactuca sativa

<400> 4

Met Cys Lys Ala Val Ser Lys Ser Pro Thr Gln Glu Tyr Phe Asp Val

1 5 10 15

Leu Gln Lys Asn Gly Leu Pro Phe Ile Asn Trp Gln Asn Asp Val Val

20 25 30

Glu Asp Glu Leu Asp Lys Glu Lys Lys Ile Leu Tyr Pro Asn Asp Glu

35 40 45

Ile Lys Gly Phe Val Glu Arg Ile Lys Val Met Leu Gly Ser Met Asp

50 55 60

Glu Gly Glu Ile Thr Val Ser Ala Tyr Asp Thr Ala Trp Val Ala Leu

65 70 75 80

Val Gln Asp Ile Asp Gly Asn Gly Arg Pro Glu Phe Pro Ser Ser Leu

85 90 95

Glu Trp Ile Val Lys Asn Gln Leu Ser Asp Gly Ser Trp Gly Asp His

100 105 110

Leu Ile Phe Ser Ala His Asp Arg Ile Ile Asn Thr Leu Ala Cys Val

115 120 125

Ile Ala Leu Thr Ser Trp Asn Val His Pro Gly Lys Cys Gln Lys Gly

130 135 140

Leu Lys Phe Leu Asn Asp Asn Ile Ser Lys Leu Glu Glu Glu Asn Pro

145 150 155 160

Glu His Met Pro Ile Gly Phe Glu Val Ala Phe Pro Ser Leu Ile Asp

165 170 175

Ile Ala Arg Lys Leu Asp Ile Gln Val Pro Glu Asp Ser Pro Ala Leu

180 185 190

Lys Glu Ile Tyr Ala Arg Arg Asn Leu Lys Leu Thr Lys Ile Pro Lys

195 200 205

Ser Leu Met His Lys Val Pro Thr Thr Leu Leu His Ser Leu Glu Gly

210 215 220

Met Pro Asp Leu Glu Trp Glu Lys Leu Leu Lys Leu Gln Cys Lys Asp

225 230 235 240

Gly Ser Phe Leu Phe Ser Pro Ser Ser Thr Ala Phe Ala Leu Met Gln

245 250 255

Thr Lys Asp Gln Lys Cys Leu Gln Tyr Leu Thr Asp Ala Val Thr Lys

260 265 270

Phe Asn Gly Gly Val Pro Asn Val Tyr Pro Val Asp Leu Phe Glu His

275 280 285

Ile Trp Val Val Asp Arg Leu Gln Arg Leu Gly Ile Ser Arg Tyr Phe

290 295 300

Asp Ser Glu Ile Lys Asp Cys Val Asp Tyr Ile Tyr Arg Tyr Trp Thr

305 310 315 320

Lys Asp Gly Ile Cys Trp Ala Lys Asn Ser Asn Val Gln Asp Ile Asp

325 330 335

Asp Thr Ala Met Gly Phe Arg Val Leu Arg Met His Gly Tyr Lys Val

340 345 350

Thr Thr Asp Val Phe Arg Gln Phe Glu Lys Asp Gly Lys Phe Val Cys

355 360 365

Phe Pro Gly Gln Thr Thr Gln Ala Val Thr Gly Met Phe Asn Leu Phe

370 375 380

Arg Ala Ser Gln Val Leu Phe Pro Asp Glu Lys Ile Leu Glu Asp Ala

385 390 395 400

Lys Lys Phe Ser Tyr Asn Tyr Leu Lys Glu Lys Gln Ser Thr Asn Glu

405 410 415

Leu Leu Asp Lys Trp Ile Ile Ala Lys Asp Leu Pro Gly Glu Val Glu

420 425 430

Tyr Ala Leu Asp Val Pro Trp Tyr Ala Ser Leu Pro Arg Leu Glu Thr

435 440 445

Arg Phe Tyr Leu Glu Gln Tyr Gly Gly Glu Asp Asp Val Trp Ile Gly

450 455 460

Lys Thr Leu Tyr Arg Met Gly Asn Val Ser Asn Asn Thr Tyr Leu Glu

465 470 475 480

Met Ala Lys Leu Asp Tyr Asn Asn Cys Leu Ala Ile His His Leu Glu

485 490 495

Trp Asn Thr Met Gln Gln Trp Tyr Val Asp Phe Gly Met Glu Arg Phe

500 505 510

Gly Thr Ser Asp Ile Thr Ser Leu Leu Val Ser Tyr Tyr Leu Ala Ala

515 520 525

Ala Ser Val Phe Glu Pro Glu Arg Ser Lys Glu Arg Ile Ala Trp Ala

530 535 540

Lys Thr Thr Thr Leu Val Asp Thr Ile Ser Ser Phe Phe His Ser Leu

545 550 555 560

Lys Ile Ser Asn Glu His Arg Arg Glu Phe Val Glu Glu Phe Arg Asn

565 570 575

Ile Ser Asn Ser Ile His His Ala Lys Tyr Gly Lys Pro Trp His Gly

580 585 590

Leu Met Val Ala Leu Lys Gly Thr Leu His Glu Ile Ala Leu Asp Val

595 600 605

Leu Met Thr His Arg Arg Asp Ile His Pro Gln Leu His His Ala Trp

610 615 620

Glu Met Trp Leu Met Arg Trp Gln Gln Gly Val Asp Ala Thr Glu Gly

625 630 635 640

Gln Ala Glu Leu Ile Val Gln Thr Ile Asn Met Thr Ala Gly Arg Trp

645 650 655

Val Ser Asn Glu Leu Leu Ala His Pro Gln Tyr Arg Leu Leu Ser Ser

660 665 670

Val Ile Asn Asn Ile Cys His Glu Ile Tyr His Asn Arg Thr Cys Met

675 680 685

Glu Val Asn Ser Thr Thr Ile Ser Thr Ser Ile Asp Ser Lys Met Gln

690 695 700

Glu Leu Val Gln Leu Val Leu Ser Asp Ser Leu Asp Asp Leu Asp Gln

705 710 715 720

Asp Leu Lys Gln Thr Phe Leu Thr Val Ala Lys Thr Phe Tyr Tyr Lys

725 730 735

Ala Tyr Cys Asp Pro Glu Thr Ile Asn Val His Ile Ser Lys Val Met

740 745 750

Phe Glu Thr Ile Ile

755

<210> 5

<211> 2283

<212> DNA

<213> Picea glauca

<220>

<221> CDS

<222> (1)..(2283)

<400> 5

atg aag atg tcc aag tct gtt gaa gtc caa cac tgt gct gtc caa ttc 48

Met Lys Met Ser Lys Ser Val Glu Val Gln His Cys Ala Val Gln Phe

1 5 10 15

ttg tct tcc acc acc gat caa atc gaa atc aga gaa aga aac ttg caa 96

Leu Ser Ser Thr Thr Asp Gln Ile Glu Ile Arg Glu Arg Asn Leu Gln

20 25 30

atc tcc act gaa gcc atg aag atg aaa tcc tgg att gaa acc gtc aaa 144

Ile Ser Thr Glu Ala Met Lys Met Lys Ser Trp Ile Glu Thr Val Lys

35 40 45

tac att ttg caa tcc atg gaa gat ggt gaa atc acc atc tcc gct tac 192

Tyr Ile Leu Gln Ser Met Glu Asp Gly Glu Ile Thr Ile Ser Ala Tyr

50 55 60

gac act gct tgg atc gcc ttg gtt cca gct ttg aac ggt tcc tct gaa 240

Asp Thr Ala Trp Ile Ala Leu Val Pro Ala Leu Asn Gly Ser Ser Glu

65 70 75 80

cct caa ttc cca tcc tct tta caa tgg ttg atc aac aac caa ttg caa 288

Pro Gln Phe Pro Ser Ser Leu Gln Trp Leu Ile Asn Asn Gln Leu Gln

85 90 95

gac ggt tcc tgg ggt gac cca ttg atg ttc ttg att aga gat cgt atc 336

Asp Gly Ser Trp Gly Asp Pro Leu Met Phe Leu Ile Arg Asp Arg Ile

100 105 110

atc aac act ttg gct tgt gtt ttg gct ttg aag acc tgg aac att cac 384

Ile Asn Thr Leu Ala Cys Val Leu Ala Leu Lys Thr Trp Asn Ile His

115 120 125

tct ttg ggt gtt aac aag ggt cta tct ttc ttg caa act tac atc cct 432

Ser Leu Gly Val Asn Lys Gly Leu Ser Phe Leu Gln Thr Tyr Ile Pro

130 135 140

aaa atg aac gat gaa cat gat gct cac act cca gtt ggt ttc gaa att 480

Lys Met Asn Asp Glu His Asp Ala His Thr Pro Val Gly Phe Glu Ile

145 150 155 160

gtc ttc cca gct ttg atg gaa gat gcc aag atc atg gaa ttg gac ttg 528

Val Phe Pro Ala Leu Met Glu Asp Ala Lys Ile Met Glu Leu Asp Leu

165 170 175

cca tac gac gct gaa ttc ttg caa aag att tac gat gaa aga gat ttg 576

Pro Tyr Asp Ala Glu Phe Leu Gln Lys Ile Tyr Asp Glu Arg Asp Leu

180 185 190

aag atg aag aga atc cca atg aag gtt ttg cac gaa ttc cca tct act 624

Lys Met Lys Arg Ile Pro Met Lys Val Leu His Glu Phe Pro Ser Thr

195 200 205

tta ttg cac tcc ttg gaa ggt ttg aga gac aag gtc aac tgg gaa gaa 672

Leu Leu His Ser Leu Glu Gly Leu Arg Asp Lys Val Asn Trp Glu Glu

210 215 220

tta ttg aag ttg caa tcc aag aac ggt tct ttc tta ttc tct cca gct 720

Leu Leu Lys Leu Gln Ser Lys Asn Gly Ser Phe Leu Phe Ser Pro Ala

225 230 235 240

tct act gct tgt gct ttg gct caa act tct gac acc aac tgt ttg aga 768

Ser Thr Ala Cys Ala Leu Ala Gln Thr Ser Asp Thr Asn Cys Leu Arg

245 250 255

tac ttg aat gaa atc acc aag aaa tac gac ggt ggt gct cca aat gtt 816

Tyr Leu Asn Glu Ile Thr Lys Lys Tyr Asp Gly Gly Ala Pro Asn Val

260 265 270

tac cca gtc gac ttg ttc gaa aga tta tgg acc gtc gat cgt att gaa 864

Tyr Pro Val Asp Leu Phe Glu Arg Leu Trp Thr Val Asp Arg Ile Glu

275 280 285

aga tta ggt att gct aga tac ttt gaa tct gaa atc act gac tct ttg 912

Arg Leu Gly Ile Ala Arg Tyr Phe Glu Ser Glu Ile Thr Asp Ser Leu

290 295 300

gaa tac gtt tac aga tac tgg act aac caa ggt atc ggt tgg gct aga 960

Glu Tyr Val Tyr Arg Tyr Trp Thr Asn Gln Gly Ile Gly Trp Ala Arg

305 310 315 320

gat tct cca gtt aag gac gtc gat gac act tcc atg gct ttc aga cta 1008

Asp Ser Pro Val Lys Asp Val Asp Asp Thr Ser Met Ala Phe Arg Leu

325 330 335

tta cgt tcc cac ggt ttc gac gtt act gct gaa gct ttc aac cac ttc 1056

Leu Arg Ser His Gly Phe Asp Val Thr Ala Glu Ala Phe Asn His Phe

340 345 350

aag caa gat gac caa ttc ttc tgt ttc ttt ggt caa acc aag caa acc 1104

Lys Gln Asp Asp Gln Phe Phe Cys Phe Phe Gly Gln Thr Lys Gln Thr

355 360 365

gtc act ggt atg tac aac ttg tac aga gct tct caa ttc tcc ttc cca 1152

Val Thr Gly Met Tyr Asn Leu Tyr Arg Ala Ser Gln Phe Ser Phe Pro

370 375 380

ggt gaa tct atc tta gaa gaa gct cgt gtc ttc acc aag aac ttc ttg 1200

Gly Glu Ser Ile Leu Glu Glu Ala Arg Val Phe Thr Lys Asn Phe Leu

385 390 395 400

gaa gaa aag aga gct gaa aag caa ttg aga gac aaa tgg atc att gct 1248

Glu Glu Lys Arg Ala Glu Lys Gln Leu Arg Asp Lys Trp Ile Ile Ala

405 410 415

aaa ggt ttg aag gaa gaa gtc gaa tac gct ttg aag ttc cca tgg tat 1296

Lys Gly Leu Lys Glu Glu Val Glu Tyr Ala Leu Lys Phe Pro Trp Tyr

420 425 430

gcc tcc caa cca aga att gac acc aga atg tac atc aac caa tac aga 1344

Ala Ser Gln Pro Arg Ile Asp Thr Arg Met Tyr Ile Asn Gln Tyr Arg

435 440 445

gtt gat gac gtc tgg atc ggt aag gct cta tac aga atg cca att gtc 1392

Val Asp Asp Val Trp Ile Gly Lys Ala Leu Tyr Arg Met Pro Ile Val

450 455 460

aac aac aag acc tac atc gaa ttg gcc aag gct gac ttc aac att tgt 1440

Asn Asn Lys Thr Tyr Ile Glu Leu Ala Lys Ala Asp Phe Asn Ile Cys

465 470 475 480

caa tct atc cac aga act gaa ttg cac ggt atc atc aga tgg tac aga 1488

Gln Ser Ile His Arg Thr Glu Leu His Gly Ile Ile Arg Trp Tyr Arg

485 490 495

gaa tcc ggt ttg gac gaa ttg ggt ttg aga caa gac caa atc gtc aaa 1536

Glu Ser Gly Leu Asp Glu Leu Gly Leu Arg Gln Asp Gln Ile Val Lys

500 505 510

tct tac ttc ttg gct gcc att gct atc tac gaa cca gac atg gcc tct 1584

Ser Tyr Phe Leu Ala Ala Ile Ala Ile Tyr Glu Pro Asp Met Ala Ser

515 520 525

gcc aga tta gct tgg gct aag tct gct gtc ttg atg gct gct atc aga 1632

Ala Arg Leu Ala Trp Ala Lys Ser Ala Val Leu Met Ala Ala Ile Arg

530 535 540

atc ttc ttt tcc ggt gaa aac tgt ttt gcc cac cat aga aga caa ttc 1680

Ile Phe Phe Ser Gly Glu Asn Cys Phe Ala His His Arg Arg Gln Phe

545 550 555 560

ttg gat gcc ttt acc aga tgg gac ggt aga gcc atg aga gat tct cca 1728

Leu Asp Ala Phe Thr Arg Trp Asp Gly Arg Ala Met Arg Asp Ser Pro

565 570 575

aac tcc gct aag aga tta ttc tct tgt tta ttc aga atg gtt aac ttg 1776

Asn Ser Ala Lys Arg Leu Phe Ser Cys Leu Phe Arg Met Val Asn Leu

580 585 590

ttc tct gtc gac ggt gtt gtt gct caa ggt aga gac atc tcc ggt gac 1824

Phe Ser Val Asp Gly Val Val Ala Gln Gly Arg Asp Ile Ser Gly Asp

595 600 605

ttg aga cac aga tgg gaa cac tgg ttg gcc tct gaa gct gaa gac ttg 1872

Leu Arg His Arg Trp Glu His Trp Leu Ala Ser Glu Ala Glu Asp Leu

610 615 620

acc gat gct caa gac cac gaa aag ttg ggt act gaa gct gaa att gtt 1920

Thr Asp Ala Gln Asp His Glu Lys Leu Gly Thr Glu Ala Glu Ile Val

625 630 635 640

gtt ttg act gct gct ttc ttg ggt aga gaa acc att tct cca gat ttg 1968

Val Leu Thr Ala Ala Phe Leu Gly Arg Glu Thr Ile Ser Pro Asp Leu

645 650 655

att tct cac cca gat ttc tct tct att atg aag gtt acc aac act gtt 2016

Ile Ser His Pro Asp Phe Ser Ser Ile Met Lys Val Thr Asn Thr Val

660 665 670

tgt tct tta ttg aga aga att gcc acc tac aag gaa gaa ggt tgt gac 2064

Cys Ser Leu Leu Arg Arg Ile Ala Thr Tyr Lys Glu Glu Gly Cys Asp

675 680 685

tcc cca tct ggt act gaa gaa gat gac aga ttg aaa cgt cgt gct gaa 2112

Ser Pro Ser Gly Thr Glu Glu Asp Asp Arg Leu Lys Arg Arg Ala Glu

690 695 700

gaa ggt atg ggt cat ttg gtt cgt gcc gtt tac aga cac caa tac tct 2160

Glu Gly Met Gly His Leu Val Arg Ala Val Tyr Arg His Gln Tyr Ser

705 710 715 720

cca gtt cca tct ggt gtc aag aga ttg tgt ttg gtt gtt ggt aag tcc 2208

Pro Val Pro Ser Gly Val Lys Arg Leu Cys Leu Val Val Gly Lys Ser

725 730 735

ttt tac tac gct gct cac tgt aac aat gaa gaa gtt ggt aac cat gtc 2256

Phe Tyr Tyr Ala Ala His Cys Asn Asn Glu Glu Val Gly Asn His Val

740 745 750

gaa acc gtc ttg ttc caa cca gta tac 2283

Glu Thr Val Leu Phe Gln Pro Val Tyr

755 760

<210> 6

<211> 761

<212> PRT

<213> Picea glauca

<400> 6

Met Lys Met Ser Lys Ser Val Glu Val Gln His Cys Ala Val Gln Phe

1 5 10 15

Leu Ser Ser Thr Thr Asp Gln Ile Glu Ile Arg Glu Arg Asn Leu Gln

20 25 30

Ile Ser Thr Glu Ala Met Lys Met Lys Ser Trp Ile Glu Thr Val Lys

35 40 45

Tyr Ile Leu Gln Ser Met Glu Asp Gly Glu Ile Thr Ile Ser Ala Tyr

50 55 60

Asp Thr Ala Trp Ile Ala Leu Val Pro Ala Leu Asn Gly Ser Ser Glu

65 70 75 80

Pro Gln Phe Pro Ser Ser Leu Gln Trp Leu Ile Asn Asn Gln Leu Gln

85 90 95

Asp Gly Ser Trp Gly Asp Pro Leu Met Phe Leu Ile Arg Asp Arg Ile

100 105 110

Ile Asn Thr Leu Ala Cys Val Leu Ala Leu Lys Thr Trp Asn Ile His

115 120 125

Ser Leu Gly Val Asn Lys Gly Leu Ser Phe Leu Gln Thr Tyr Ile Pro

130 135 140

Lys Met Asn Asp Glu His Asp Ala His Thr Pro Val Gly Phe Glu Ile

145 150 155 160

Val Phe Pro Ala Leu Met Glu Asp Ala Lys Ile Met Glu Leu Asp Leu

165 170 175

Pro Tyr Asp Ala Glu Phe Leu Gln Lys Ile Tyr Asp Glu Arg Asp Leu

180 185 190

Lys Met Lys Arg Ile Pro Met Lys Val Leu His Glu Phe Pro Ser Thr

195 200 205

Leu Leu His Ser Leu Glu Gly Leu Arg Asp Lys Val Asn Trp Glu Glu

210 215 220

Leu Leu Lys Leu Gln Ser Lys Asn Gly Ser Phe Leu Phe Ser Pro Ala

225 230 235 240

Ser Thr Ala Cys Ala Leu Ala Gln Thr Ser Asp Thr Asn Cys Leu Arg

245 250 255

Tyr Leu Asn Glu Ile Thr Lys Lys Tyr Asp Gly Gly Ala Pro Asn Val

260 265 270

Tyr Pro Val Asp Leu Phe Glu Arg Leu Trp Thr Val Asp Arg Ile Glu

275 280 285

Arg Leu Gly Ile Ala Arg Tyr Phe Glu Ser Glu Ile Thr Asp Ser Leu

290 295 300

Glu Tyr Val Tyr Arg Tyr Trp Thr Asn Gln Gly Ile Gly Trp Ala Arg

305 310 315 320

Asp Ser Pro Val Lys Asp Val Asp Asp Thr Ser Met Ala Phe Arg Leu

325 330 335

Leu Arg Ser His Gly Phe Asp Val Thr Ala Glu Ala Phe Asn His Phe

340 345 350

Lys Gln Asp Asp Gln Phe Phe Cys Phe Phe Gly Gln Thr Lys Gln Thr

355 360 365

Val Thr Gly Met Tyr Asn Leu Tyr Arg Ala Ser Gln Phe Ser Phe Pro

370 375 380

Gly Glu Ser Ile Leu Glu Glu Ala Arg Val Phe Thr Lys Asn Phe Leu

385 390 395 400

Glu Glu Lys Arg Ala Glu Lys Gln Leu Arg Asp Lys Trp Ile Ile Ala

405 410 415

Lys Gly Leu Lys Glu Glu Val Glu Tyr Ala Leu Lys Phe Pro Trp Tyr

420 425 430

Ala Ser Gln Pro Arg Ile Asp Thr Arg Met Tyr Ile Asn Gln Tyr Arg

435 440 445

Val Asp Asp Val Trp Ile Gly Lys Ala Leu Tyr Arg Met Pro Ile Val

450 455 460

Asn Asn Lys Thr Tyr Ile Glu Leu Ala Lys Ala Asp Phe Asn Ile Cys

465 470 475 480

Gln Ser Ile His Arg Thr Glu Leu His Gly Ile Ile Arg Trp Tyr Arg

485 490 495

Glu Ser Gly Leu Asp Glu Leu Gly Leu Arg Gln Asp Gln Ile Val Lys

500 505 510

Ser Tyr Phe Leu Ala Ala Ile Ala Ile Tyr Glu Pro Asp Met Ala Ser

515 520 525

Ala Arg Leu Ala Trp Ala Lys Ser Ala Val Leu Met Ala Ala Ile Arg

530 535 540

Ile Phe Phe Ser Gly Glu Asn Cys Phe Ala His His Arg Arg Gln Phe

545 550 555 560

Leu Asp Ala Phe Thr Arg Trp Asp Gly Arg Ala Met Arg Asp Ser Pro

565 570 575

Asn Ser Ala Lys Arg Leu Phe Ser Cys Leu Phe Arg Met Val Asn Leu

580 585 590

Phe Ser Val Asp Gly Val Val Ala Gln Gly Arg Asp Ile Ser Gly Asp

595 600 605

Leu Arg His Arg Trp Glu His Trp Leu Ala Ser Glu Ala Glu Asp Leu

610 615 620

Thr Asp Ala Gln Asp His Glu Lys Leu Gly Thr Glu Ala Glu Ile Val

625 630 635 640

Val Leu Thr Ala Ala Phe Leu Gly Arg Glu Thr Ile Ser Pro Asp Leu

645 650 655

Ile Ser His Pro Asp Phe Ser Ser Ile Met Lys Val Thr Asn Thr Val

660 665 670

Cys Ser Leu Leu Arg Arg Ile Ala Thr Tyr Lys Glu Glu Gly Cys Asp

675 680 685

Ser Pro Ser Gly Thr Glu Glu Asp Asp Arg Leu Lys Arg Arg Ala Glu

690 695 700

Glu Gly Met Gly His Leu Val Arg Ala Val Tyr Arg His Gln Tyr Ser

705 710 715 720

Pro Val Pro Ser Gly Val Lys Arg Leu Cys Leu Val Val Gly Lys Ser

725 730 735

Phe Tyr Tyr Ala Ala His Cys Asn Asn Glu Glu Val Gly Asn His Val

740 745 750

Glu Thr Val Leu Phe Gln Pro Val Tyr

755 760

<210> 7

<211> 1548

<212> DNA

<213> Bradyrhizobium japonicum

<220>

<221> CDS

<222> (1)..(1548)

<400> 7

atg aac gct ttg tct gaa cac atc tta tct gaa ttg aga aga tta ttg 48

Met Asn Ala Leu Ser Glu His Ile Leu Ser Glu Leu Arg Arg Leu Leu

1 5 10 15

tcc gaa atg tct gac ggt ggt tcc gtc ggt cct tcc gtt tac gac acc 96

Ser Glu Met Ser Asp Gly Gly Ser Val Gly Pro Ser Val Tyr Asp Thr

20 25 30

gcc caa gcc ttg aga ttc cac ggt aac gtc act ggt aga caa gat gct 144

Ala Gln Ala Leu Arg Phe His Gly Asn Val Thr Gly Arg Gln Asp Ala

35 40 45

tac gct tgg ttg att gct caa caa caa gct gac ggt ggt tgg ggt tct 192

Tyr Ala Trp Leu Ile Ala Gln Gln Gln Ala Asp Gly Gly Trp Gly Ser

50 55 60

gct gat ttc cca tta ttc aga cat gcc cca acc tgg gct gct cta ttg 240

Ala Asp Phe Pro Leu Phe Arg His Ala Pro Thr Trp Ala Ala Leu Leu

65 70 75 80

gct ttg caa aga gct gac cca ttg cca ggt gct gct gat gcc gtc caa 288

Ala Leu Gln Arg Ala Asp Pro Leu Pro Gly Ala Ala Asp Ala Val Gln

85 90 95

acc gcc acc aga ttc ttg caa aga caa cca gac cca tac gct cat gcc 336

Thr Ala Thr Arg Phe Leu Gln Arg Gln Pro Asp Pro Tyr Ala His Ala

100 105 110

gtt cca gaa gat gct cca atc ggt gct gaa ttg atc ttg cct caa ttc 384

Val Pro Glu Asp Ala Pro Ile Gly Ala Glu Leu Ile Leu Pro Gln Phe

115 120 125

tgt ggt gaa gct gct tct cta tta ggt ggt gtt gcc ttc cca aga cac 432

Cys Gly Glu Ala Ala Ser Leu Leu Gly Gly Val Ala Phe Pro Arg His

130 135 140

cca gct ttg ttg cca ttg aga caa gct tgt ttg gtc aaa ttg ggt gcc 480

Pro Ala Leu Leu Pro Leu Arg Gln Ala Cys Leu Val Lys Leu Gly Ala

145 150 155 160

gtt gcc atg ttg cca tct ggt cac cca ttg ttg cac tcc tgg gaa gcc 528

Val Ala Met Leu Pro Ser Gly His Pro Leu Leu His Ser Trp Glu Ala

165 170 175

tgg ggt act tct cca act act gct tgt cca gac gat gac ggt tcc atc 576

Trp Gly Thr Ser Pro Thr Thr Ala Cys Pro Asp Asp Asp Gly Ser Ile

180 185 190

ggt atc tct cca gct gct act gct gct tgg aga gcc caa gcc gtt acc 624

Gly Ile Ser Pro Ala Ala Thr Ala Ala Trp Arg Ala Gln Ala Val Thr

195 200 205

aga ggt tcc act cca caa gtc ggt aga gct gat gct tac ttg caa atg 672

Arg Gly Ser Thr Pro Gln Val Gly Arg Ala Asp Ala Tyr Leu Gln Met

210 215 220

gct tcc aga gcc acc aga tct ggt att gaa ggt gtt ttc cca aat gtc 720

Ala Ser Arg Ala Thr Arg Ser Gly Ile Glu Gly Val Phe Pro Asn Val

225 230 235 240

tgg cca atc aac gtc ttt gaa cca tgt tgg tct ttg tac act ttg cac 768

Trp Pro Ile Asn Val Phe Glu Pro Cys Trp Ser Leu Tyr Thr Leu His

245 250 255

ttg gct ggt ttg ttt gct cat cca gct ttg gct gaa gcc gtc aga gtc 816

Leu Ala Gly Leu Phe Ala His Pro Ala Leu Ala Glu Ala Val Arg Val

260 265 270

att gtt gct caa ttg gat gct aga tta ggt gtc cac ggt tta ggt cca 864

Ile Val Ala Gln Leu Asp Ala Arg Leu Gly Val His Gly Leu Gly Pro

275 280 285

gct ttg cac ttc gct gct gac gct gat gac acc gcc gtt gct cta tgt 912

Ala Leu His Phe Ala Ala Asp Ala Asp Asp Thr Ala Val Ala Leu Cys

290 295 300

gtt ttg cac ttg gct ggt cgt gac cca gct gtt gac gct ttg aga cac 960

Val Leu His Leu Ala Gly Arg Asp Pro Ala Val Asp Ala Leu Arg His

305 310 315 320

ttc gaa att ggt gaa ttg ttc gtc act ttc cca ggt gaa aga aac gct 1008

Phe Glu Ile Gly Glu Leu Phe Val Thr Phe Pro Gly Glu Arg Asn Ala

325 330 335

tct gtt tcc acc aac atc cac gct ttg cac gct ttg aga ttg ttg ggt 1056

Ser Val Ser Thr Asn Ile His Ala Leu His Ala Leu Arg Leu Leu Gly

340 345 350

aag cca gct gct ggt gct tct gct tac gtt gaa gcc aac aga aac cca 1104

Lys Pro Ala Ala Gly Ala Ser Ala Tyr Val Glu Ala Asn Arg Asn Pro

355 360 365

cac ggt tta tgg gac aac gaa aag tgg cac gtt tcc tgg tta tac cca 1152

His Gly Leu Trp Asp Asn Glu Lys Trp His Val Ser Trp Leu Tyr Pro

370 375 380

act gct cac gcc gtt gct gct ttg gcc caa ggt aag cct caa tgg aga 1200

Thr Ala His Ala Val Ala Ala Leu Ala Gln Gly Lys Pro Gln Trp Arg

385 390 395 400

gat gaa aga gct ttg gcc gct ttg ttg caa gct caa aga gat gac ggt 1248

Asp Glu Arg Ala Leu Ala Ala Leu Leu Gln Ala Gln Arg Asp Asp Gly

405 410 415

ggt tgg ggt gct ggt cgt ggt tct act ttc gaa gaa acc gcc tac gct 1296

Gly Trp Gly Ala Gly Arg Gly Ser Thr Phe Glu Glu Thr Ala Tyr Ala

420 425 430

ttg ttt gct ttg cat gtc atg gac ggt tct gaa gaa gct acc ggt aga 1344

Leu Phe Ala Leu His Val Met Asp Gly Ser Glu Glu Ala Thr Gly Arg

435 440 445

aga aga att gct caa gtt gtt gcc aga gct ttg gaa tgg atg ttg gct 1392

Arg Arg Ile Ala Gln Val Val Ala Arg Ala Leu Glu Trp Met Leu Ala

450 455 460

cgt cac gct gcc cac ggt ttg cca caa act cca tta tgg atc ggt aag 1440

Arg His Ala Ala His Gly Leu Pro Gln Thr Pro Leu Trp Ile Gly Lys

465 470 475 480

gaa ttg tac tgt cca acc aga gtt gtc aga gtt gct gaa ttg gct ggt 1488

Glu Leu Tyr Cys Pro Thr Arg Val Val Arg Val Ala Glu Leu Ala Gly

485 490 495

tta tgg ttg gct ttg aga tgg ggt cgt cgt gtc ttg gct gaa ggt gct 1536

Leu Trp Leu Ala Leu Arg Trp Gly Arg Arg Val Leu Ala Glu Gly Ala

500 505 510

ggt gct gca ccc 1548

Gly Ala Ala Pro

515

<210> 8

<211> 516

<212> PRT

<213> Bradyrhizobium japonicum

<400> 8

Met Asn Ala Leu Ser Glu His Ile Leu Ser Glu Leu Arg Arg Leu Leu

1 5 10 15

Ser Glu Met Ser Asp Gly Gly Ser Val Gly Pro Ser Val Tyr Asp Thr

20 25 30

Ala Gln Ala Leu Arg Phe His Gly Asn Val Thr Gly Arg Gln Asp Ala

35 40 45

Tyr Ala Trp Leu Ile Ala Gln Gln Gln Ala Asp Gly Gly Trp Gly Ser

50 55 60

Ala Asp Phe Pro Leu Phe Arg His Ala Pro Thr Trp Ala Ala Leu Leu

65 70 75 80

Ala Leu Gln Arg Ala Asp Pro Leu Pro Gly Ala Ala Asp Ala Val Gln

85 90 95

Thr Ala Thr Arg Phe Leu Gln Arg Gln Pro Asp Pro Tyr Ala His Ala

100 105 110

Val Pro Glu Asp Ala Pro Ile Gly Ala Glu Leu Ile Leu Pro Gln Phe

115 120 125

Cys Gly Glu Ala Ala Ser Leu Leu Gly Gly Val Ala Phe Pro Arg His

130 135 140

Pro Ala Leu Leu Pro Leu Arg Gln Ala Cys Leu Val Lys Leu Gly Ala

145 150 155 160

Val Ala Met Leu Pro Ser Gly His Pro Leu Leu His Ser Trp Glu Ala

165 170 175

Trp Gly Thr Ser Pro Thr Thr Ala Cys Pro Asp Asp Asp Gly Ser Ile

180 185 190

Gly Ile Ser Pro Ala Ala Thr Ala Ala Trp Arg Ala Gln Ala Val Thr

195 200 205

Arg Gly Ser Thr Pro Gln Val Gly Arg Ala Asp Ala Tyr Leu Gln Met

210 215 220

Ala Ser Arg Ala Thr Arg Ser Gly Ile Glu Gly Val Phe Pro Asn Val

225 230 235 240

Trp Pro Ile Asn Val Phe Glu Pro Cys Trp Ser Leu Tyr Thr Leu His

245 250 255

Leu Ala Gly Leu Phe Ala His Pro Ala Leu Ala Glu Ala Val Arg Val

260 265 270

Ile Val Ala Gln Leu Asp Ala Arg Leu Gly Val His Gly Leu Gly Pro

275 280 285

Ala Leu His Phe Ala Ala Asp Ala Asp Asp Thr Ala Val Ala Leu Cys

290 295 300

Val Leu His Leu Ala Gly Arg Asp Pro Ala Val Asp Ala Leu Arg His

305 310 315 320

Phe Glu Ile Gly Glu Leu Phe Val Thr Phe Pro Gly Glu Arg Asn Ala

325 330 335

Ser Val Ser Thr Asn Ile His Ala Leu His Ala Leu Arg Leu Leu Gly

340 345 350

Lys Pro Ala Ala Gly Ala Ser Ala Tyr Val Glu Ala Asn Arg Asn Pro

355 360 365

His Gly Leu Trp Asp Asn Glu Lys Trp His Val Ser Trp Leu Tyr Pro

370 375 380

Thr Ala His Ala Val Ala Ala Leu Ala Gln Gly Lys Pro Gln Trp Arg

385 390 395 400

Asp Glu Arg Ala Leu Ala Ala Leu Leu Gln Ala Gln Arg Asp Asp Gly

405 410 415

Gly Trp Gly Ala Gly Arg Gly Ser Thr Phe Glu Glu Thr Ala Tyr Ala

420 425 430

Leu Phe Ala Leu His Val Met Asp Gly Ser Glu Glu Ala Thr Gly Arg

435 440 445

Arg Arg Ile Ala Gln Val Val Ala Arg Ala Leu Glu Trp Met Leu Ala

450 455 460

Arg His Ala Ala His Gly Leu Pro Gln Thr Pro Leu Trp Ile Gly Lys

465 470 475 480

Glu Leu Tyr Cys Pro Thr Arg Val Val Arg Val Ala Glu Leu Ala Gly

485 490 495

Leu Trp Leu Ala Leu Arg Trp Gly Arg Arg Val Leu Ala Glu Gly Ala

500 505 510

Gly Ala Ala Pro

515

<210> 9

<211> 2364

<212> DNA

<213> Lactuca sativa

<220>

<221> CDS

<222> (1)..(2364)

<400> 9

atg aac att gct caa atc acc tct tct gct atg ttg gtt cca tct tct 48

Met Asn Ile Ala Gln Ile Thr Ser Ser Ala Met Leu Val Pro Ser Ser

1 5 10 15

cac atc cca cac cgt tcc tgg gtt gtc aac tgt tgt atg gtt caa tac 96

His Ile Pro His Arg Ser Trp Val Val Asn Cys Cys Met Val Gln Tyr

20 25 30

aac cca tcc ggt cta aga act gct tcc tct caa gcc ggt caa gtc aac 144

Asn Pro Ser Gly Leu Arg Thr Ala Ser Ser Gln Ala Gly Gln Val Asn

35 40 45

cca act gtt atg act ttg gat gtc act aag gaa aga atc aga aag ttg 192

Pro Thr Val Met Thr Leu Asp Val Thr Lys Glu Arg Ile Arg Lys Leu

50 55 60

ttc aac aac gtc gaa gtt tct gtt tcc tct tac gac acc gct tgg gtt 240

Phe Asn Asn Val Glu Val Ser Val Ser Ser Tyr Asp Thr Ala Trp Val

65 70 75 80

gcc atg gtt cca tct cca aac tct cca aag tcc cca tgt ttc cca gac 288

Ala Met Val Pro Ser Pro Asn Ser Pro Lys Ser Pro Cys Phe Pro Asp

85 90 95

tgt ttg aac tgg ttg ttg gac aac caa ttg gat gat ggt tcc tgg ggt 336

Cys Leu Asn Trp Leu Leu Asp Asn Gln Leu Asp Asp Gly Ser Trp Gly

100 105 110

ttg ttg cca cac caa tct cca ttg atc aaa gac act tta tct tct act 384

Leu Leu Pro His Gln Ser Pro Leu Ile Lys Asp Thr Leu Ser Ser Thr

115 120 125

ttg gct tgt gtt ttg gct tta aag aga tgg aac gtc ggt aag gat caa 432

Leu Ala Cys Val Leu Ala Leu Lys Arg Trp Asn Val Gly Lys Asp Gln

130 135 140

atc aac aag ggt tta cat tac atc gaa tcc aac ttt gct tcc gtc act 480

Ile Asn Lys Gly Leu His Tyr Ile Glu Ser Asn Phe Ala Ser Val Thr

145 150 155 160

gat aag aac caa gcc tct cca ttc ggt ttt gac atc att ttc cca ggt 528

Asp Lys Asn Gln Ala Ser Pro Phe Gly Phe Asp Ile Ile Phe Pro Gly

165 170 175

atg ttg gaa tac gcc aag gat ttg gac att aaa ttg cct ttg aac caa 576

Met Leu Glu Tyr Ala Lys Asp Leu Asp Ile Lys Leu Pro Leu Asn Gln

180 185 190

act cac ttg tcc gtc atg ttg cac gaa aga gaa ttg gaa ttg aga aga 624

Thr His Leu Ser Val Met Leu His Glu Arg Glu Leu Glu Leu Arg Arg

195 200 205

tgt cac tct aac ggt cgt gaa gcc tac tta gct tac att tcc gaa ggt 672

Cys His Ser Asn Gly Arg Glu Ala Tyr Leu Ala Tyr Ile Ser Glu Gly

210 215 220

ttg ggt aac ttg aac gac tgg aac atg gtt atg aag tac caa atg aag 720

Leu Gly Asn Leu Asn Asp Trp Asn Met Val Met Lys Tyr Gln Met Lys

225 230 235 240

aac ggt tct ttg ttc aac tct cct tct gct act gct tct gtt ttg att 768

Asn Gly Ser Leu Phe Asn Ser Pro Ser Ala Thr Ala Ser Val Leu Ile

245 250 255

cac cac caa aat gct ggt tgt ttg cac tac tta acc tcc cta ttg gac 816

His His Gln Asn Ala Gly Cys Leu His Tyr Leu Thr Ser Leu Leu Asp

260 265 270

aag ttt ggt aat gct gtc cca act gtt tac cca atc gac ttg tac gtt 864

Lys Phe Gly Asn Ala Val Pro Thr Val Tyr Pro Ile Asp Leu Tyr Val

275 280 285

aga ttg tcc atg gtt gac act ttg gaa aga ttg ggt atc aag aga cat 912

Arg Leu Ser Met Val Asp Thr Leu Glu Arg Leu Gly Ile Lys Arg His

290 295 300

ttc atg gtt gaa att caa aat gtc ttg gac gaa acc tac aga tgt tgg 960

Phe Met Val Glu Ile Gln Asn Val Leu Asp Glu Thr Tyr Arg Cys Trp

305 310 315 320

gtt caa ggt gat gtt caa atc ttc atg gat gtc gtt acc tgt gct ttg 1008

Val Gln Gly Asp Val Gln Ile Phe Met Asp Val Val Thr Cys Ala Leu

325 330 335

gct ttc aga gtt cta cgt tct aac ggt tac gaa gtc tct tct gac cca 1056

Ala Phe Arg Val Leu Arg Ser Asn Gly Tyr Glu Val Ser Ser Asp Pro

340 345 350

ttg gct aag att acc aaa gaa ggt gac tac atg aac tct cca gaa aag 1104

Leu Ala Lys Ile Thr Lys Glu Gly Asp Tyr Met Asn Ser Pro Glu Lys

355 360 365

cct ttc aag gac gtc tac acc tct ttg gaa gtc tac aag gct tct caa 1152

Pro Phe Lys Asp Val Tyr Thr Ser Leu Glu Val Tyr Lys Ala Ser Gln

370 375 380

atc atc tac caa gaa gaa ttg gcc ttc aga gaa caa aac ttg acc tct 1200

Ile Ile Tyr Gln Glu Glu Leu Ala Phe Arg Glu Gln Asn Leu Thr Ser

385 390 395 400

tat ttg cca tct tct aac aaa ttg tcc aac tac att ttg aag gaa gtt 1248

Tyr Leu Pro Ser Ser Asn Lys Leu Ser Asn Tyr Ile Leu Lys Glu Val

405 410 415

gat gac gct ttg aag ttc cca ttc aat ggt tct cta gaa aga atg tcc 1296

Asp Asp Ala Leu Lys Phe Pro Phe Asn Gly Ser Leu Glu Arg Met Ser

420 425 430

act aga aga aac att gaa cac tac aac ttg aac cac acc aga att ttg 1344

Thr Arg Arg Asn Ile Glu His Tyr Asn Leu Asn His Thr Arg Ile Leu

435 440 445

aag acc acc tac tcc tct tcc aac att tcc aac aag gac tac ttg aaa 1392

Lys Thr Thr Tyr Ser Ser Ser Asn Ile Ser Asn Lys Asp Tyr Leu Lys

450 455 460

tta gcc gtc caa gac ttc aac gaa tgt caa tcc atc tac tgt gaa gaa 1440

Leu Ala Val Gln Asp Phe Asn Glu Cys Gln Ser Ile Tyr Cys Glu Glu

465 470 475 480

tta aag gac ttg gaa aga tgg gtt gtc gaa aac aga tta gac aag ttg 1488

Leu Lys Asp Leu Glu Arg Trp Val Val Glu Asn Arg Leu Asp Lys Leu

485 490 495

aaa ttt gct cgt caa aag acc gct tac tgt tac ttc tct gct gct tct 1536

Lys Phe Ala Arg Gln Lys Thr Ala Tyr Cys Tyr Phe Ser Ala Ala Ser

500 505 510

ttc tta tct tct cca gat ttg tcc gat gct aga atc tcc tgg gct aaa 1584

Phe Leu Ser Ser Pro Asp Leu Ser Asp Ala Arg Ile Ser Trp Ala Lys

515 520 525

tct tcc att ttg act acc gtc att gat gat ttc ttt gac gtc ggt ggt 1632

Ser Ser Ile Leu Thr Thr Val Ile Asp Asp Phe Phe Asp Val Gly Gly

530 535 540

tcc atg gat gaa ttg gtt aac ttc gtc cac atc atc gaa aag tgg aac 1680

Ser Met Asp Glu Leu Val Asn Phe Val His Ile Ile Glu Lys Trp Asn

545 550 555 560

gtc aac gtt gaa aac gat tgt tgt tct gaa gaa gtc ggt gtt ttg ttc 1728

Val Asn Val Glu Asn Asp Cys Cys Ser Glu Glu Val Gly Val Leu Phe

565 570 575

ttg gct tta aag gat gct gtc tgt tgg att ggt gac aag gct ttc aag 1776

Leu Ala Leu Lys Asp Ala Val Cys Trp Ile Gly Asp Lys Ala Phe Lys

580 585 590

atc caa gaa aga aac atc act tcc cac gtc att gaa atc tgg ttg gac 1824

Ile Gln Glu Arg Asn Ile Thr Ser His Val Ile Glu Ile Trp Leu Asp

595 600 605

ttg gtt aag tcc atg ttg aga gaa gct att tgg gct aag gac ggt tcc 1872

Leu Val Lys Ser Met Leu Arg Glu Ala Ile Trp Ala Lys Asp Gly Ser

610 615 620

atc cca acc atc aac gaa tac atg gaa aac ggt tac gtt tct ttc gcc 1920

Ile Pro Thr Ile Asn Glu Tyr Met Glu Asn Gly Tyr Val Ser Phe Ala

625 630 635 640

ttg ggt cca atc gtt ttg cca act ttg tac ttc tta ggt gtc aag ttg 1968

Leu Gly Pro Ile Val Leu Pro Thr Leu Tyr Phe Leu Gly Val Lys Leu

645 650 655

tct gaa gaa gtc gtc caa tct tcc gaa tac cac aag ttg tac gaa gtt 2016

Ser Glu Glu Val Val Gln Ser Ser Glu Tyr His Lys Leu Tyr Glu Val

660 665 670

atg tcc acc caa ggt aga ttg atg aac gac att cac tct ttc aaa cgt 2064

Met Ser Thr Gln Gly Arg Leu Met Asn Asp Ile His Ser Phe Lys Arg

675 680 685

gaa aag aag gcc ggt aag ttg aat gct gtt gct tta tac atg tcc gac 2112

Glu Lys Lys Ala Gly Lys Leu Asn Ala Val Ala Leu Tyr Met Ser Asp

690 695 700

ggt aaa tct ggt tct gtt gaa gaa gaa gtt gtc gaa gaa atg aag atc 2160

Gly Lys Ser Gly Ser Val Glu Glu Glu Val Val Glu Glu Met Lys Ile

705 710 715 720

ttg act aaa tct caa aga aag gaa atg atg aag ttg gtt ttg gaa acc 2208

Leu Thr Lys Ser Gln Arg Lys Glu Met Met Lys Leu Val Leu Glu Thr

725 730 735

aag ggt tcc gtt gtc cca aga gtt tgt aag gac gtt ttc tgg aac atg 2256

Lys Gly Ser Val Val Pro Arg Val Cys Lys Asp Val Phe Trp Asn Met

740 745 750

tgt aac gtc ttg aac ttg ttc tac gct acc gat gac ggt ttc act ggt 2304

Cys Asn Val Leu Asn Leu Phe Tyr Ala Thr Asp Asp Gly Phe Thr Gly

755 760 765

aac gcc atc tta gat gtt gtc aag gaa atc atc tac gaa cca gtc tct 2352

Asn Ala Ile Leu Asp Val Val Lys Glu Ile Ile Tyr Glu Pro Val Ser

770 775 780

cat gaa ttg ata 2364

His Glu Leu Ile

785

<210> 10

<211> 788

<212> PRT

<213> Lactuca sativa

<400> 10

Met Asn Ile Ala Gln Ile Thr Ser Ser Ala Met Leu Val Pro Ser Ser

1 5 10 15

His Ile Pro His Arg Ser Trp Val Val Asn Cys Cys Met Val Gln Tyr

20 25 30

Asn Pro Ser Gly Leu Arg Thr Ala Ser Ser Gln Ala Gly Gln Val Asn

35 40 45

Pro Thr Val Met Thr Leu Asp Val Thr Lys Glu Arg Ile Arg Lys Leu

50 55 60

Phe Asn Asn Val Glu Val Ser Val Ser Ser Tyr Asp Thr Ala Trp Val

65 70 75 80

Ala Met Val Pro Ser Pro Asn Ser Pro Lys Ser Pro Cys Phe Pro Asp

85 90 95

Cys Leu Asn Trp Leu Leu Asp Asn Gln Leu Asp Asp Gly Ser Trp Gly

100 105 110

Leu Leu Pro His Gln Ser Pro Leu Ile Lys Asp Thr Leu Ser Ser Thr

115 120 125

Leu Ala Cys Val Leu Ala Leu Lys Arg Trp Asn Val Gly Lys Asp Gln

130 135 140

Ile Asn Lys Gly Leu His Tyr Ile Glu Ser Asn Phe Ala Ser Val Thr

145 150 155 160

Asp Lys Asn Gln Ala Ser Pro Phe Gly Phe Asp Ile Ile Phe Pro Gly

165 170 175

Met Leu Glu Tyr Ala Lys Asp Leu Asp Ile Lys Leu Pro Leu Asn Gln

180 185 190

Thr His Leu Ser Val Met Leu His Glu Arg Glu Leu Glu Leu Arg Arg

195 200 205

Cys His Ser Asn Gly Arg Glu Ala Tyr Leu Ala Tyr Ile Ser Glu Gly

210 215 220

Leu Gly Asn Leu Asn Asp Trp Asn Met Val Met Lys Tyr Gln Met Lys

225 230 235 240

Asn Gly Ser Leu Phe Asn Ser Pro Ser Ala Thr Ala Ser Val Leu Ile

245 250 255

His His Gln Asn Ala Gly Cys Leu His Tyr Leu Thr Ser Leu Leu Asp

260 265 270

Lys Phe Gly Asn Ala Val Pro Thr Val Tyr Pro Ile Asp Leu Tyr Val

275 280 285

Arg Leu Ser Met Val Asp Thr Leu Glu Arg Leu Gly Ile Lys Arg His

290 295 300

Phe Met Val Glu Ile Gln Asn Val Leu Asp Glu Thr Tyr Arg Cys Trp

305 310 315 320

Val Gln Gly Asp Val Gln Ile Phe Met Asp Val Val Thr Cys Ala Leu

325 330 335

Ala Phe Arg Val Leu Arg Ser Asn Gly Tyr Glu Val Ser Ser Asp Pro

340 345 350

Leu Ala Lys Ile Thr Lys Glu Gly Asp Tyr Met Asn Ser Pro Glu Lys

355 360 365

Pro Phe Lys Asp Val Tyr Thr Ser Leu Glu Val Tyr Lys Ala Ser Gln

370 375 380

Ile Ile Tyr Gln Glu Glu Leu Ala Phe Arg Glu Gln Asn Leu Thr Ser

385 390 395 400

Tyr Leu Pro Ser Ser Asn Lys Leu Ser Asn Tyr Ile Leu Lys Glu Val

405 410 415

Asp Asp Ala Leu Lys Phe Pro Phe Asn Gly Ser Leu Glu Arg Met Ser

420 425 430

Thr Arg Arg Asn Ile Glu His Tyr Asn Leu Asn His Thr Arg Ile Leu

435 440 445

Lys Thr Thr Tyr Ser Ser Ser Asn Ile Ser Asn Lys Asp Tyr Leu Lys

450 455 460

Leu Ala Val Gln Asp Phe Asn Glu Cys Gln Ser Ile Tyr Cys Glu Glu

465 470 475 480

Leu Lys Asp Leu Glu Arg Trp Val Val Glu Asn Arg Leu Asp Lys Leu

485 490 495

Lys Phe Ala Arg Gln Lys Thr Ala Tyr Cys Tyr Phe Ser Ala Ala Ser

500 505 510

Phe Leu Ser Ser Pro Asp Leu Ser Asp Ala Arg Ile Ser Trp Ala Lys

515 520 525

Ser Ser Ile Leu Thr Thr Val Ile Asp Asp Phe Phe Asp Val Gly Gly

530 535 540

Ser Met Asp Glu Leu Val Asn Phe Val His Ile Ile Glu Lys Trp Asn

545 550 555 560

Val Asn Val Glu Asn Asp Cys Cys Ser Glu Glu Val Gly Val Leu Phe

565 570 575

Leu Ala Leu Lys Asp Ala Val Cys Trp Ile Gly Asp Lys Ala Phe Lys

580 585 590

Ile Gln Glu Arg Asn Ile Thr Ser His Val Ile Glu Ile Trp Leu Asp

595 600 605

Leu Val Lys Ser Met Leu Arg Glu Ala Ile Trp Ala Lys Asp Gly Ser

610 615 620

Ile Pro Thr Ile Asn Glu Tyr Met Glu Asn Gly Tyr Val Ser Phe Ala

625 630 635 640

Leu Gly Pro Ile Val Leu Pro Thr Leu Tyr Phe Leu Gly Val Lys Leu

645 650 655

Ser Glu Glu Val Val Gln Ser Ser Glu Tyr His Lys Leu Tyr Glu Val

660 665 670

Met Ser Thr Gln Gly Arg Leu Met Asn Asp Ile His Ser Phe Lys Arg

675 680 685

Glu Lys Lys Ala Gly Lys Leu Asn Ala Val Ala Leu Tyr Met Ser Asp

690 695 700

Gly Lys Ser Gly Ser Val Glu Glu Glu Val Val Glu Glu Met Lys Ile

705 710 715 720

Leu Thr Lys Ser Gln Arg Lys Glu Met Met Lys Leu Val Leu Glu Thr

725 730 735

Lys Gly Ser Val Val Pro Arg Val Cys Lys Asp Val Phe Trp Asn Met

740 745 750

Cys Asn Val Leu Asn Leu Phe Tyr Ala Thr Asp Asp Gly Phe Thr Gly

755 760 765

Asn Ala Ile Leu Asp Val Val Lys Glu Ile Ile Tyr Glu Pro Val Ser

770 775 780

His Glu Leu Ile

785

<210> 11

<211> 2250

<212> DNA

<213> Lactuca sativa

<220>

<221> CDS

<222> (1)..(2250)

<400> 11

atg gct tcc tct caa gcc ggt caa gtc aac cca act gtt atg act ttg 48

Met Ala Ser Ser Gln Ala Gly Gln Val Asn Pro Thr Val Met Thr Leu

1 5 10 15

gat gtc act aag gaa aga atc aga aag ttg ttc aac aac gtc gaa gtt 96

Asp Val Thr Lys Glu Arg Ile Arg Lys Leu Phe Asn Asn Val Glu Val

20 25 30

tct gtt tcc tct tac gac acc gct tgg gtt gcc atg gtt cca tct cca 144

Ser Val Ser Ser Tyr Asp Thr Ala Trp Val Ala Met Val Pro Ser Pro

35 40 45

aac tct cca aag tcc cca tgt ttc cca gac tgt ttg aac tgg ttg ttg 192

Asn Ser Pro Lys Ser Pro Cys Phe Pro Asp Cys Leu Asn Trp Leu Leu

50 55 60

gac aac caa ttg gat gat ggt tcc tgg ggt ttg ttg cca cac caa tct 240

Asp Asn Gln Leu Asp Asp Gly Ser Trp Gly Leu Leu Pro His Gln Ser

65 70 75 80

cca ttg atc aaa gac act tta tct tct act ttg gct tgt gtt ttg gct 288

Pro Leu Ile Lys Asp Thr Leu Ser Ser Thr Leu Ala Cys Val Leu Ala

85 90 95

tta aag aga tgg aac gtc ggt aag gat caa atc aac aag ggt tta cat 336

Leu Lys Arg Trp Asn Val Gly Lys Asp Gln Ile Asn Lys Gly Leu His

100 105 110

tac atc gaa tcc aac ttt gct tcc gtc act gat aag aac caa gcc tct 384

Tyr Ile Glu Ser Asn Phe Ala Ser Val Thr Asp Lys Asn Gln Ala Ser

115 120 125

cca ttc ggt ttt gac atc att ttc cca ggt atg ttg gaa tac gcc aag 432

Pro Phe Gly Phe Asp Ile Ile Phe Pro Gly Met Leu Glu Tyr Ala Lys

130 135 140

gat ttg gac att aaa ttg cct ttg aac caa act cac ttg tcc gtc atg 480

Asp Leu Asp Ile Lys Leu Pro Leu Asn Gln Thr His Leu Ser Val Met

145 150 155 160

ttg cac gaa aga gaa ttg gaa ttg aga aga tgt cac tct aac ggt cgt 528

Leu His Glu Arg Glu Leu Glu Leu Arg Arg Cys His Ser Asn Gly Arg

165 170 175

gaa gcc tac tta gct tac att tcc gaa ggt ttg ggt aac ttg aac gac 576

Glu Ala Tyr Leu Ala Tyr Ile Ser Glu Gly Leu Gly Asn Leu Asn Asp

180 185 190

tgg aac atg gtt atg aag tac caa atg aag aac ggt tct ttg ttc aac 624

Trp Asn Met Val Met Lys Tyr Gln Met Lys Asn Gly Ser Leu Phe Asn

195 200 205

tct cct tct gct act gct tct gtt ttg att cac cac caa aat gct ggt 672

Ser Pro Ser Ala Thr Ala Ser Val Leu Ile His His Gln Asn Ala Gly

210 215 220

tgt ttg cac tac tta acc tcc cta ttg gac aag ttt ggt aat gct gtc 720

Cys Leu His Tyr Leu Thr Ser Leu Leu Asp Lys Phe Gly Asn Ala Val

225 230 235 240

cca act gtt tac cca atc gac ttg tac gtt aga ttg tcc atg gtt gac 768

Pro Thr Val Tyr Pro Ile Asp Leu Tyr Val Arg Leu Ser Met Val Asp

245 250 255

act ttg gaa aga ttg ggt atc aag aga cat ttc atg gtt gaa att caa 816

Thr Leu Glu Arg Leu Gly Ile Lys Arg His Phe Met Val Glu Ile Gln

260 265 270

aat gtc ttg gac gaa acc tac aga tgt tgg gtt caa ggt gat gtt caa 864

Asn Val Leu Asp Glu Thr Tyr Arg Cys Trp Val Gln Gly Asp Val Gln

275 280 285

atc ttc atg gat gtc gtt acc tgt gct ttg gct ttc aga gtt cta cgt 912

Ile Phe Met Asp Val Val Thr Cys Ala Leu Ala Phe Arg Val Leu Arg

290 295 300

tct aac ggt tac gaa gtc tct tct gac cca ttg gct aag att acc aaa 960

Ser Asn Gly Tyr Glu Val Ser Ser Asp Pro Leu Ala Lys Ile Thr Lys

305 310 315 320

gaa ggt gac tac atg aac tct cca gaa aag cct ttc aag gac gtc tac 1008

Glu Gly Asp Tyr Met Asn Ser Pro Glu Lys Pro Phe Lys Asp Val Tyr

325 330 335

acc tct ttg gaa gtc tac aag gct tct caa atc atc tac caa gaa gaa 1056

Thr Ser Leu Glu Val Tyr Lys Ala Ser Gln Ile Ile Tyr Gln Glu Glu

340 345 350

ttg gcc ttc aga gaa caa aac ttg acc tct tat ttg cca tct tct aac 1104

Leu Ala Phe Arg Glu Gln Asn Leu Thr Ser Tyr Leu Pro Ser Ser Asn

355 360 365

aaa ttg tcc aac tac att ttg aag gaa gtt gat gac gct ttg aag ttc 1152

Lys Leu Ser Asn Tyr Ile Leu Lys Glu Val Asp Asp Ala Leu Lys Phe

370 375 380

cca ttc aat ggt tct cta gaa aga atg tcc act aga aga aac att gaa 1200

Pro Phe Asn Gly Ser Leu Glu Arg Met Ser Thr Arg Arg Asn Ile Glu

385 390 395 400

cac tac aac ttg aac cac acc aga att ttg aag acc acc tac tcc tct 1248

His Tyr Asn Leu Asn His Thr Arg Ile Leu Lys Thr Thr Tyr Ser Ser

405 410 415

tcc aac att tcc aac aag gac tac ttg aaa tta gcc gtc caa gac ttc 1296

Ser Asn Ile Ser Asn Lys Asp Tyr Leu Lys Leu Ala Val Gln Asp Phe

420 425 430

aac gaa tgt caa tcc atc tac tgt gaa gaa tta aag gac ttg gaa aga 1344

Asn Glu Cys Gln Ser Ile Tyr Cys Glu Glu Leu Lys Asp Leu Glu Arg

435 440 445

tgg gtt gtc gaa aac aga tta gac aag ttg aaa ttt gct cgt caa aag 1392

Trp Val Val Glu Asn Arg Leu Asp Lys Leu Lys Phe Ala Arg Gln Lys

450 455 460

acc gct tac tgt tac ttc tct gct gct tct ttc tta tct tct cca gat 1440

Thr Ala Tyr Cys Tyr Phe Ser Ala Ala Ser Phe Leu Ser Ser Pro Asp

465 470 475 480

ttg tcc gat gct aga atc tcc tgg gct aaa tct tcc att ttg act acc 1488

Leu Ser Asp Ala Arg Ile Ser Trp Ala Lys Ser Ser Ile Leu Thr Thr

485 490 495

gtc att gat gat ttc ttt gac gtc ggt ggt tcc atg gat gaa ttg gtt 1536

Val Ile Asp Asp Phe Phe Asp Val Gly Gly Ser Met Asp Glu Leu Val

500 505 510

aac ttc gtc cac atc atc gaa aag tgg aac gtc aac gtt gaa aac gat 1584

Asn Phe Val His Ile Ile Glu Lys Trp Asn Val Asn Val Glu Asn Asp

515 520 525

tgt tgt tct gaa gaa gtc ggt gtt ttg ttc ttg gct tta aag gat gct 1632

Cys Cys Ser Glu Glu Val Gly Val Leu Phe Leu Ala Leu Lys Asp Ala

530 535 540

gtc tgt tgg att ggt gac aag gct ttc aag atc caa gaa aga aac atc 1680

Val Cys Trp Ile Gly Asp Lys Ala Phe Lys Ile Gln Glu Arg Asn Ile

545 550 555 560

act tcc cac gtc att gaa atc tgg ttg gac ttg gtt aag tcc atg ttg 1728

Thr Ser His Val Ile Glu Ile Trp Leu Asp Leu Val Lys Ser Met Leu

565 570 575

aga gaa gct att tgg gct aag gac ggt tcc atc cca acc atc aac gaa 1776

Arg Glu Ala Ile Trp Ala Lys Asp Gly Ser Ile Pro Thr Ile Asn Glu

580 585 590

tac atg gaa aac ggt tac gtt tct ttc gcc ttg ggt cca atc gtt ttg 1824

Tyr Met Glu Asn Gly Tyr Val Ser Phe Ala Leu Gly Pro Ile Val Leu

595 600 605

cca act ttg tac ttc tta ggt gtc aag ttg tct gaa gaa gtc gtc caa 1872

Pro Thr Leu Tyr Phe Leu Gly Val Lys Leu Ser Glu Glu Val Val Gln

610 615 620

tct tcc gaa tac cac aag ttg tac gaa gtt atg tcc acc caa ggt aga 1920

Ser Ser Glu Tyr His Lys Leu Tyr Glu Val Met Ser Thr Gln Gly Arg

625 630 635 640

ttg atg aac gac att cac tct ttc aaa cgt gaa aag aag gcc ggt aag 1968

Leu Met Asn Asp Ile His Ser Phe Lys Arg Glu Lys Lys Ala Gly Lys

645 650 655

ttg aat gct gtt gct tta tac atg tcc gac ggt aaa tct ggt tct gtt 2016

Leu Asn Ala Val Ala Leu Tyr Met Ser Asp Gly Lys Ser Gly Ser Val

660 665 670

gaa gaa gaa gtt gtc gaa gaa atg aag atc ttg act aaa tct caa aga 2064

Glu Glu Glu Val Val Glu Glu Met Lys Ile Leu Thr Lys Ser Gln Arg

675 680 685

aag gaa atg atg aag ttg gtt ttg gaa acc aag ggt tcc gtt gtc cca 2112

Lys Glu Met Met Lys Leu Val Leu Glu Thr Lys Gly Ser Val Val Pro

690 695 700

aga gtt tgt aag gac gtt ttc tgg aac atg tgt aac gtc ttg aac ttg 2160

Arg Val Cys Lys Asp Val Phe Trp Asn Met Cys Asn Val Leu Asn Leu

705 710 715 720

ttc tac gct acc gat gac ggt ttc act ggt aac gcc atc tta gat gtt 2208

Phe Tyr Ala Thr Asp Asp Gly Phe Thr Gly Asn Ala Ile Leu Asp Val

725 730 735

gtc aag gaa atc atc tac gaa cca gtc tct cat gaa ttg ata 2250

Val Lys Glu Ile Ile Tyr Glu Pro Val Ser His Glu Leu Ile

740 745 750

<210> 12

<211> 750

<212> PRT

<213> Lactuca sativa

<400> 12

Met Ala Ser Ser Gln Ala Gly Gln Val Asn Pro Thr Val Met Thr Leu

1 5 10 15

Asp Val Thr Lys Glu Arg Ile Arg Lys Leu Phe Asn Asn Val Glu Val

20 25 30

Ser Val Ser Ser Tyr Asp Thr Ala Trp Val Ala Met Val Pro Ser Pro

35 40 45

Asn Ser Pro Lys Ser Pro Cys Phe Pro Asp Cys Leu Asn Trp Leu Leu

50 55 60

Asp Asn Gln Leu Asp Asp Gly Ser Trp Gly Leu Leu Pro His Gln Ser

65 70 75 80

Pro Leu Ile Lys Asp Thr Leu Ser Ser Thr Leu Ala Cys Val Leu Ala

85 90 95

Leu Lys Arg Trp Asn Val Gly Lys Asp Gln Ile Asn Lys Gly Leu His

100 105 110

Tyr Ile Glu Ser Asn Phe Ala Ser Val Thr Asp Lys Asn Gln Ala Ser

115 120 125

Pro Phe Gly Phe Asp Ile Ile Phe Pro Gly Met Leu Glu Tyr Ala Lys

130 135 140

Asp Leu Asp Ile Lys Leu Pro Leu Asn Gln Thr His Leu Ser Val Met

145 150 155 160

Leu His Glu Arg Glu Leu Glu Leu Arg Arg Cys His Ser Asn Gly Arg

165 170 175

Glu Ala Tyr Leu Ala Tyr Ile Ser Glu Gly Leu Gly Asn Leu Asn Asp

180 185 190

Trp Asn Met Val Met Lys Tyr Gln Met Lys Asn Gly Ser Leu Phe Asn

195 200 205

Ser Pro Ser Ala Thr Ala Ser Val Leu Ile His His Gln Asn Ala Gly

210 215 220

Cys Leu His Tyr Leu Thr Ser Leu Leu Asp Lys Phe Gly Asn Ala Val

225 230 235 240

Pro Thr Val Tyr Pro Ile Asp Leu Tyr Val Arg Leu Ser Met Val Asp

245 250 255

Thr Leu Glu Arg Leu Gly Ile Lys Arg His Phe Met Val Glu Ile Gln

260 265 270

Asn Val Leu Asp Glu Thr Tyr Arg Cys Trp Val Gln Gly Asp Val Gln

275 280 285

Ile Phe Met Asp Val Val Thr Cys Ala Leu Ala Phe Arg Val Leu Arg

290 295 300

Ser Asn Gly Tyr Glu Val Ser Ser Asp Pro Leu Ala Lys Ile Thr Lys

305 310 315 320

Glu Gly Asp Tyr Met Asn Ser Pro Glu Lys Pro Phe Lys Asp Val Tyr

325 330 335

Thr Ser Leu Glu Val Tyr Lys Ala Ser Gln Ile Ile Tyr Gln Glu Glu

340 345 350

Leu Ala Phe Arg Glu Gln Asn Leu Thr Ser Tyr Leu Pro Ser Ser Asn

355 360 365

Lys Leu Ser Asn Tyr Ile Leu Lys Glu Val Asp Asp Ala Leu Lys Phe

370 375 380

Pro Phe Asn Gly Ser Leu Glu Arg Met Ser Thr Arg Arg Asn Ile Glu

385 390 395 400

His Tyr Asn Leu Asn His Thr Arg Ile Leu Lys Thr Thr Tyr Ser Ser

405 410 415

Ser Asn Ile Ser Asn Lys Asp Tyr Leu Lys Leu Ala Val Gln Asp Phe

420 425 430

Asn Glu Cys Gln Ser Ile Tyr Cys Glu Glu Leu Lys Asp Leu Glu Arg

435 440 445

Trp Val Val Glu Asn Arg Leu Asp Lys Leu Lys Phe Ala Arg Gln Lys

450 455 460

Thr Ala Tyr Cys Tyr Phe Ser Ala Ala Ser Phe Leu Ser Ser Pro Asp

465 470 475 480

Leu Ser Asp Ala Arg Ile Ser Trp Ala Lys Ser Ser Ile Leu Thr Thr

485 490 495

Val Ile Asp Asp Phe Phe Asp Val Gly Gly Ser Met Asp Glu Leu Val

500 505 510

Asn Phe Val His Ile Ile Glu Lys Trp Asn Val Asn Val Glu Asn Asp

515 520 525

Cys Cys Ser Glu Glu Val Gly Val Leu Phe Leu Ala Leu Lys Asp Ala

530 535 540

Val Cys Trp Ile Gly Asp Lys Ala Phe Lys Ile Gln Glu Arg Asn Ile

545 550 555 560

Thr Ser His Val Ile Glu Ile Trp Leu Asp Leu Val Lys Ser Met Leu

565 570 575

Arg Glu Ala Ile Trp Ala Lys Asp Gly Ser Ile Pro Thr Ile Asn Glu

580 585 590

Tyr Met Glu Asn Gly Tyr Val Ser Phe Ala Leu Gly Pro Ile Val Leu

595 600 605

Pro Thr Leu Tyr Phe Leu Gly Val Lys Leu Ser Glu Glu Val Val Gln

610 615 620

Ser Ser Glu Tyr His Lys Leu Tyr Glu Val Met Ser Thr Gln Gly Arg

625 630 635 640

Leu Met Asn Asp Ile His Ser Phe Lys Arg Glu Lys Lys Ala Gly Lys

645 650 655

Leu Asn Ala Val Ala Leu Tyr Met Ser Asp Gly Lys Ser Gly Ser Val

660 665 670

Glu Glu Glu Val Val Glu Glu Met Lys Ile Leu Thr Lys Ser Gln Arg

675 680 685

Lys Glu Met Met Lys Leu Val Leu Glu Thr Lys Gly Ser Val Val Pro

690 695 700

Arg Val Cys Lys Asp Val Phe Trp Asn Met Cys Asn Val Leu Asn Leu

705 710 715 720

Phe Tyr Ala Thr Asp Asp Gly Phe Thr Gly Asn Ala Ile Leu Asp Val

725 730 735

Val Lys Glu Ile Ile Tyr Glu Pro Val Ser His Glu Leu Ile

740 745 750

<210> 13

<211> 2271

<212> DNA

<213> Picea glauca

<220>

<221> CDS

<222> (1)..(2271)

<400> 13

atg aag aga gaa caa tac act atc ttg aac gaa aag gaa tcc atg gct 48

Met Lys Arg Glu Gln Tyr Thr Ile Leu Asn Glu Lys Glu Ser Met Ala

1 5 10 15

gaa gaa tta atc ttg aga atc aag aga atg ttt tct gaa atc gaa aac 96

Glu Glu Leu Ile Leu Arg Ile Lys Arg Met Phe Ser Glu Ile Glu Asn

20 25 30

act caa acc tct gct tct gct tac gac acc gct tgg gtt gcc atg gtt 144

Thr Gln Thr Ser Ala Ser Ala Tyr Asp Thr Ala Trp Val Ala Met Val

35 40 45

cca tct ttg gac tcc tct caa caa cca caa ttc cct caa tgt ttg tcc 192

Pro Ser Leu Asp Ser Ser Gln Gln Pro Gln Phe Pro Gln Cys Leu Ser

50 55 60

tgg atc att gac aac caa ttg ttg gat ggt tcc tgg ggt atc cca tac 240

Trp Ile Ile Asp Asn Gln Leu Leu Asp Gly Ser Trp Gly Ile Pro Tyr

65 70 75 80

ttg atc atc aag gac aga cta tgt cac act tta gct tgt gtc att gct 288

Leu Ile Ile Lys Asp Arg Leu Cys His Thr Leu Ala Cys Val Ile Ala

85 90 95

ttg aga aaa tgg aac gct ggt aac caa aat gtc gaa acc ggt cta cgt 336

Leu Arg Lys Trp Asn Ala Gly Asn Gln Asn Val Glu Thr Gly Leu Arg

100 105 110

ttc ttg aga gaa aac att gaa ggt atc gtt cac gaa gat gaa tac act 384

Phe Leu Arg Glu Asn Ile Glu Gly Ile Val His Glu Asp Glu Tyr Thr

115 120 125

cca att ggt ttc caa atc att ttc cca gct atg ttg gaa gaa gct cgt 432

Pro Ile Gly Phe Gln Ile Ile Phe Pro Ala Met Leu Glu Glu Ala Arg

130 135 140

ggt tta ggt cta gaa ttg cca tac gac ttg act cca atc aag ttg atg 480

Gly Leu Gly Leu Glu Leu Pro Tyr Asp Leu Thr Pro Ile Lys Leu Met

145 150 155 160

ttg acc cac aga gaa aag atc atg aag ggt aag gcc att gac cac atg 528

Leu Thr His Arg Glu Lys Ile Met Lys Gly Lys Ala Ile Asp His Met

165 170 175

cac gaa tac gac tct tct ttg atc tac act gtt gaa ggt atc cac aag 576

His Glu Tyr Asp Ser Ser Leu Ile Tyr Thr Val Glu Gly Ile His Lys

180 185 190

atc gtt gac tgg aac aag gtt ttg aaa cat caa aac aag gat ggt tct 624

Ile Val Asp Trp Asn Lys Val Leu Lys His Gln Asn Lys Asp Gly Ser

195 200 205

ttg ttc aac tct cca tct gct act gct tgt gct ttg atg cac act aga 672

Leu Phe Asn Ser Pro Ser Ala Thr Ala Cys Ala Leu Met His Thr Arg

210 215 220

aag tcc aac tgt ttg gaa tac ttg tcc tcc atg ttg caa aag tta ggt 720

Lys Ser Asn Cys Leu Glu Tyr Leu Ser Ser Met Leu Gln Lys Leu Gly

225 230 235 240

aac ggt gtt cca tcc gtc tac cca atc aac tta tat gct cgt atc tcc 768

Asn Gly Val Pro Ser Val Tyr Pro Ile Asn Leu Tyr Ala Arg Ile Ser

245 250 255

atg att gac aga tta caa aga tta ggt ttg gct aga cat ttc aga aac 816

Met Ile Asp Arg Leu Gln Arg Leu Gly Leu Ala Arg His Phe Arg Asn

260 265 270

gaa atc att cac gct ttg gac gac atc tac aga tac tgg atg caa aag 864

Glu Ile Ile His Ala Leu Asp Asp Ile Tyr Arg Tyr Trp Met Gln Lys

275 280 285

gaa acc tcc aga gaa ggt aag tct ttg acc cca gat att gtc tct act 912

Glu Thr Ser Arg Glu Gly Lys Ser Leu Thr Pro Asp Ile Val Ser Thr

290 295 300

tcc atc gct ttc atg ttg ttg aga ttg cac ggt tac gat gtt cca gct 960

Ser Ile Ala Phe Met Leu Leu Arg Leu His Gly Tyr Asp Val Pro Ala

305 310 315 320

gat gtt ttc tgt tgt tac cat ttg cac tcc att gaa caa tct ggt gaa 1008

Asp Val Phe Cys Cys Tyr His Leu His Ser Ile Glu Gln Ser Gly Glu

325 330 335

gcc gtt act gct atg ttg tct ttg tac aga gcc tct caa att atg ttc 1056

Ala Val Thr Ala Met Leu Ser Leu Tyr Arg Ala Ser Gln Ile Met Phe

340 345 350

cca ggt gaa acc att ttg gaa gaa atc aag acc gtt tct aga aag tac 1104

Pro Gly Glu Thr Ile Leu Glu Glu Ile Lys Thr Val Ser Arg Lys Tyr

355 360 365

ttg gac aag aga aag gaa aac ggt cgt atc tac tac cac aac att gtt 1152

Leu Asp Lys Arg Lys Glu Asn Gly Arg Ile Tyr Tyr His Asn Ile Val

370 375 380

atg aag gac ttg aga ggt gaa gtc gaa tac gcc ttg tct gtt cca tgg 1200

Met Lys Asp Leu Arg Gly Glu Val Glu Tyr Ala Leu Ser Val Pro Trp

385 390 395 400

tac gct tcc ttg gaa aga att gaa aac aga aga tac att gac caa tac 1248

Tyr Ala Ser Leu Glu Arg Ile Glu Asn Arg Arg Tyr Ile Asp Gln Tyr

405 410 415

ggt gtc aac gat acc tgg atc gct aag acc tct tac aaa atc cca tgt 1296

Gly Val Asn Asp Thr Trp Ile Ala Lys Thr Ser Tyr Lys Ile Pro Cys

420 425 430

atc tcc aat gac tta ttc ttg gct ttg gcc aag caa gac tac aac atc 1344

Ile Ser Asn Asp Leu Phe Leu Ala Leu Ala Lys Gln Asp Tyr Asn Ile

435 440 445

tgt caa gcc att caa caa aag gaa ttg aga gaa ttg gaa aga tgg ttt 1392

Cys Gln Ala Ile Gln Gln Lys Glu Leu Arg Glu Leu Glu Arg Trp Phe

450 455 460

gct gat aac aaa ttc tct cac ttg aac ttc gct cgt caa aag ttg atc 1440

Ala Asp Asn Lys Phe Ser His Leu Asn Phe Ala Arg Gln Lys Leu Ile

465 470 475 480

tac tgt tac ttc tct gcc gct gct act ttg ttc tct cca gaa ttg tct 1488

Tyr Cys Tyr Phe Ser Ala Ala Ala Thr Leu Phe Ser Pro Glu Leu Ser

485 490 495

gct gcc aga gtt gtc tgg gct aag aac ggt gtt atc acc acc gtc gtt 1536

Ala Ala Arg Val Val Trp Ala Lys Asn Gly Val Ile Thr Thr Val Val

500 505 510

gat gac ttc ttc gat gtc ggt ggt tct tct gaa gaa att cac tct ttc 1584

Asp Asp Phe Phe Asp Val Gly Gly Ser Ser Glu Glu Ile His Ser Phe

515 520 525

gtc gaa gct gtt aga gtc tgg gac gaa gct gct acc gat ggt ttg tct 1632

Val Glu Ala Val Arg Val Trp Asp Glu Ala Ala Thr Asp Gly Leu Ser

530 535 540

gaa aac gtt caa atc tta ttc tcc gct tta tac aac acc gtc gac gaa 1680

Glu Asn Val Gln Ile Leu Phe Ser Ala Leu Tyr Asn Thr Val Asp Glu

545 550 555 560

att gtc caa caa gcc ttc gtt ttc caa ggt aga gac atc tcc att cac 1728

Ile Val Gln Gln Ala Phe Val Phe Gln Gly Arg Asp Ile Ser Ile His

565 570 575

ttg aga gaa atc tgg tac aga ttg gtc aac tcc atg atg act gaa gct 1776

Leu Arg Glu Ile Trp Tyr Arg Leu Val Asn Ser Met Met Thr Glu Ala

580 585 590

caa tgg gct aga act cac tgt att cca tcc atg cac gaa tac atg gaa 1824

Gln Trp Ala Arg Thr His Cys Ile Pro Ser Met His Glu Tyr Met Glu

595 600 605

aac gct gaa cct tct att gct ttg gaa cca atc gtc ttg tcc tct tta 1872

Asn Ala Glu Pro Ser Ile Ala Leu Glu Pro Ile Val Leu Ser Ser Leu

610 615 620

tac ttt gtt ggt cca aag ttg tct gaa gaa atc att tgt cac cca gaa 1920

Tyr Phe Val Gly Pro Lys Leu Ser Glu Glu Ile Ile Cys His Pro Glu

625 630 635 640

tac tac aac ttg atg cat ttg ttg aat atc tgt ggt aga ttg ttg aac 1968

Tyr Tyr Asn Leu Met His Leu Leu Asn Ile Cys Gly Arg Leu Leu Asn

645 650 655

gat atc caa ggt tgt aaa cgt gaa gcc cac caa ggt aag ttg aac tcc 2016

Asp Ile Gln Gly Cys Lys Arg Glu Ala His Gln Gly Lys Leu Asn Ser

660 665 670

gtc act ttg tac atg gaa gaa aac tct ggt act acc atg gaa gat gct 2064

Val Thr Leu Tyr Met Glu Glu Asn Ser Gly Thr Thr Met Glu Asp Ala

675 680 685

atc gtt tac ttg aga aag acc atc gac gaa tcc aga caa ttg cta ttg 2112

Ile Val Tyr Leu Arg Lys Thr Ile Asp Glu Ser Arg Gln Leu Leu Leu

690 695 700

aaa gaa gtt tta aga cca tct att gtt cca aga gaa tgt aaa caa ttg 2160

Lys Glu Val Leu Arg Pro Ser Ile Val Pro Arg Glu Cys Lys Gln Leu

705 710 715 720

cac tgg aac atg atg aga atc ttg caa ttg ttc tac ttg aag aac gat 2208

His Trp Asn Met Met Arg Ile Leu Gln Leu Phe Tyr Leu Lys Asn Asp

725 730 735

ggt ttt acc tct cca act gaa atg ttg ggt tac gtc aac gcc gtc att 2256

Gly Phe Thr Ser Pro Thr Glu Met Leu Gly Tyr Val Asn Ala Val Ile

740 745 750

gtc gac cca att ttg 2271

Val Asp Pro Ile Leu

755

<210> 14

<211> 757

<212> PRT

<213> Picea glauca

<400> 14

Met Lys Arg Glu Gln Tyr Thr Ile Leu Asn Glu Lys Glu Ser Met Ala

1 5 10 15

Glu Glu Leu Ile Leu Arg Ile Lys Arg Met Phe Ser Glu Ile Glu Asn

20 25 30

Thr Gln Thr Ser Ala Ser Ala Tyr Asp Thr Ala Trp Val Ala Met Val

35 40 45

Pro Ser Leu Asp Ser Ser Gln Gln Pro Gln Phe Pro Gln Cys Leu Ser

50 55 60

Trp Ile Ile Asp Asn Gln Leu Leu Asp Gly Ser Trp Gly Ile Pro Tyr

65 70 75 80

Leu Ile Ile Lys Asp Arg Leu Cys His Thr Leu Ala Cys Val Ile Ala

85 90 95

Leu Arg Lys Trp Asn Ala Gly Asn Gln Asn Val Glu Thr Gly Leu Arg

100 105 110

Phe Leu Arg Glu Asn Ile Glu Gly Ile Val His Glu Asp Glu Tyr Thr

115 120 125

Pro Ile Gly Phe Gln Ile Ile Phe Pro Ala Met Leu Glu Glu Ala Arg

130 135 140

Gly Leu Gly Leu Glu Leu Pro Tyr Asp Leu Thr Pro Ile Lys Leu Met

145 150 155 160

Leu Thr His Arg Glu Lys Ile Met Lys Gly Lys Ala Ile Asp His Met

165 170 175

His Glu Tyr Asp Ser Ser Leu Ile Tyr Thr Val Glu Gly Ile His Lys

180 185 190

Ile Val Asp Trp Asn Lys Val Leu Lys His Gln Asn Lys Asp Gly Ser

195 200 205

Leu Phe Asn Ser Pro Ser Ala Thr Ala Cys Ala Leu Met His Thr Arg

210 215 220

Lys Ser Asn Cys Leu Glu Tyr Leu Ser Ser Met Leu Gln Lys Leu Gly

225 230 235 240

Asn Gly Val Pro Ser Val Tyr Pro Ile Asn Leu Tyr Ala Arg Ile Ser

245 250 255

Met Ile Asp Arg Leu Gln Arg Leu Gly Leu Ala Arg His Phe Arg Asn

260 265 270

Glu Ile Ile His Ala Leu Asp Asp Ile Tyr Arg Tyr Trp Met Gln Lys

275 280 285

Glu Thr Ser Arg Glu Gly Lys Ser Leu Thr Pro Asp Ile Val Ser Thr

290 295 300

Ser Ile Ala Phe Met Leu Leu Arg Leu His Gly Tyr Asp Val Pro Ala

305 310 315 320

Asp Val Phe Cys Cys Tyr His Leu His Ser Ile Glu Gln Ser Gly Glu

325 330 335

Ala Val Thr Ala Met Leu Ser Leu Tyr Arg Ala Ser Gln Ile Met Phe

340 345 350

Pro Gly Glu Thr Ile Leu Glu Glu Ile Lys Thr Val Ser Arg Lys Tyr

355 360 365

Leu Asp Lys Arg Lys Glu Asn Gly Arg Ile Tyr Tyr His Asn Ile Val

370 375 380

Met Lys Asp Leu Arg Gly Glu Val Glu Tyr Ala Leu Ser Val Pro Trp

385 390 395 400

Tyr Ala Ser Leu Glu Arg Ile Glu Asn Arg Arg Tyr Ile Asp Gln Tyr

405 410 415

Gly Val Asn Asp Thr Trp Ile Ala Lys Thr Ser Tyr Lys Ile Pro Cys

420 425 430

Ile Ser Asn Asp Leu Phe Leu Ala Leu Ala Lys Gln Asp Tyr Asn Ile

435 440 445

Cys Gln Ala Ile Gln Gln Lys Glu Leu Arg Glu Leu Glu Arg Trp Phe

450 455 460

Ala Asp Asn Lys Phe Ser His Leu Asn Phe Ala Arg Gln Lys Leu Ile

465 470 475 480

Tyr Cys Tyr Phe Ser Ala Ala Ala Thr Leu Phe Ser Pro Glu Leu Ser

485 490 495

Ala Ala Arg Val Val Trp Ala Lys Asn Gly Val Ile Thr Thr Val Val

500 505 510

Asp Asp Phe Phe Asp Val Gly Gly Ser Ser Glu Glu Ile His Ser Phe

515 520 525

Val Glu Ala Val Arg Val Trp Asp Glu Ala Ala Thr Asp Gly Leu Ser

530 535 540

Glu Asn Val Gln Ile Leu Phe Ser Ala Leu Tyr Asn Thr Val Asp Glu

545 550 555 560

Ile Val Gln Gln Ala Phe Val Phe Gln Gly Arg Asp Ile Ser Ile His

565 570 575

Leu Arg Glu Ile Trp Tyr Arg Leu Val Asn Ser Met Met Thr Glu Ala

580 585 590

Gln Trp Ala Arg Thr His Cys Ile Pro Ser Met His Glu Tyr Met Glu

595 600 605

Asn Ala Glu Pro Ser Ile Ala Leu Glu Pro Ile Val Leu Ser Ser Leu

610 615 620

Tyr Phe Val Gly Pro Lys Leu Ser Glu Glu Ile Ile Cys His Pro Glu

625 630 635 640

Tyr Tyr Asn Leu Met His Leu Leu Asn Ile Cys Gly Arg Leu Leu Asn

645 650 655

Asp Ile Gln Gly Cys Lys Arg Glu Ala His Gln Gly Lys Leu Asn Ser

660 665 670

Val Thr Leu Tyr Met Glu Glu Asn Ser Gly Thr Thr Met Glu Asp Ala

675 680 685

Ile Val Tyr Leu Arg Lys Thr Ile Asp Glu Ser Arg Gln Leu Leu Leu

690 695 700

Lys Glu Val Leu Arg Pro Ser Ile Val Pro Arg Glu Cys Lys Gln Leu

705 710 715 720

His Trp Asn Met Met Arg Ile Leu Gln Leu Phe Tyr Leu Lys Asn Asp

725 730 735

Gly Phe Thr Ser Pro Thr Glu Met Leu Gly Tyr Val Asn Ala Val Ile

740 745 750

Val Asp Pro Ile Leu

755

<210> 15

<211> 900

<212> DNA

<213> Bradyrhizobium japonicum

<220>

<221> CDS

<222> (1)..(900)

<400> 15

atg atc caa act gaa aga gct gtc caa caa gtt ttg gaa tgg ggt cgt 48

Met Ile Gln Thr Glu Arg Ala Val Gln Gln Val Leu Glu Trp Gly Arg

1 5 10 15

tct ttg acc ggt ttc gct gac gaa cac gct gtc gaa gct gtt cgt ggt 96

Ser Leu Thr Gly Phe Ala Asp Glu His Ala Val Glu Ala Val Arg Gly

20 25 30

ggt caa tac atc tta caa aga atc cac cca tct ttg aga ggt act tct 144

Gly Gln Tyr Ile Leu Gln Arg Ile His Pro Ser Leu Arg Gly Thr Ser

35 40 45

gcc aga act ggt aga gat cca caa gat gaa act ttg att gtc acc ttt 192

Ala Arg Thr Gly Arg Asp Pro Gln Asp Glu Thr Leu Ile Val Thr Phe

50 55 60

tac aga gaa ttg gct ttg ttg ttc tgg tta gat gac tgt aac gat ttg 240

Tyr Arg Glu Leu Ala Leu Leu Phe Trp Leu Asp Asp Cys Asn Asp Leu

65 70 75 80

ggt ttg att tcc cca gaa caa ttg gct gct gtc gaa caa gct ttg ggt 288

Gly Leu Ile Ser Pro Glu Gln Leu Ala Ala Val Glu Gln Ala Leu Gly

85 90 95

caa ggt gtc cca tgt gct ttg cca ggt ttc gaa ggt tgt gcc gtt ttg 336

Gln Gly Val Pro Cys Ala Leu Pro Gly Phe Glu Gly Cys Ala Val Leu

100 105 110

aga gct tct ttg gcc act ttg gcc tac gac aga aga gac tac gct caa 384

Arg Ala Ser Leu Ala Thr Leu Ala Tyr Asp Arg Arg Asp Tyr Ala Gln

115 120 125

ttg ttg gat gac acc aga tgt tac tcc gct gct ttg aga gct ggt cat 432

Leu Leu Asp Asp Thr Arg Cys Tyr Ser Ala Ala Leu Arg Ala Gly His

130 135 140

gcc caa gct gtt gct gct gaa aga tgg tcc tac gct gaa tac ttg cac 480

Ala Gln Ala Val Ala Ala Glu Arg Trp Ser Tyr Ala Glu Tyr Leu His

145 150 155 160

aac ggt att gac tcc att gct tac gct aac gtt ttc tgt tgt ttg tcc 528

Asn Gly Ile Asp Ser Ile Ala Tyr Ala Asn Val Phe Cys Cys Leu Ser

165 170 175

cta tta tgg ggt ttg gac atg gct act ttg aga gcc aga cca gct ttc 576

Leu Leu Trp Gly Leu Asp Met Ala Thr Leu Arg Ala Arg Pro Ala Phe

180 185 190

aga caa gtc ttg aga tta atc tct gcc att ggt aga tta caa aac gat 624

Arg Gln Val Leu Arg Leu Ile Ser Ala Ile Gly Arg Leu Gln Asn Asp

195 200 205

ttg cac ggt tgt gac aag gac cgt tct gct ggt gaa gct gac aac gct 672

Leu His Gly Cys Asp Lys Asp Arg Ser Ala Gly Glu Ala Asp Asn Ala

210 215 220

gtc atc tta ttg ttg caa aga tac cca gcc atg cca gtt gtt gaa ttc 720

Val Ile Leu Leu Leu Gln Arg Tyr Pro Ala Met Pro Val Val Glu Phe

225 230 235 240

ttg aac gat gaa ttg gct ggt cac acc aga atg ttg cac cgt gtc atg 768

Leu Asn Asp Glu Leu Ala Gly His Thr Arg Met Leu His Arg Val Met

245 250 255

gct gaa gaa aga ttc cca gct cca tgg ggt cca tta atc gaa gct atg 816

Ala Glu Glu Arg Phe Pro Ala Pro Trp Gly Pro Leu Ile Glu Ala Met

260 265 270

gct gcc atc aga gtt caa tac tac aga acc tcc acc tct cgt tac aga 864

Ala Ala Ile Arg Val Gln Tyr Tyr Arg Thr Ser Thr Ser Arg Tyr Arg

275 280 285

tct gac gct gtt aga ggt ggt caa aga gcc cct gcc 900

Ser Asp Ala Val Arg Gly Gly Gln Arg Ala Pro Ala

290 295 300

<210> 16

<211> 300

<212> PRT

<213> Bradyrhizobium japonicum

<400> 16

Met Ile Gln Thr Glu Arg Ala Val Gln Gln Val Leu Glu Trp Gly Arg

1 5 10 15

Ser Leu Thr Gly Phe Ala Asp Glu His Ala Val Glu Ala Val Arg Gly

20 25 30

Gly Gln Tyr Ile Leu Gln Arg Ile His Pro Ser Leu Arg Gly Thr Ser

35 40 45

Ala Arg Thr Gly Arg Asp Pro Gln Asp Glu Thr Leu Ile Val Thr Phe

50 55 60

Tyr Arg Glu Leu Ala Leu Leu Phe Trp Leu Asp Asp Cys Asn Asp Leu

65 70 75 80

Gly Leu Ile Ser Pro Glu Gln Leu Ala Ala Val Glu Gln Ala Leu Gly

85 90 95

Gln Gly Val Pro Cys Ala Leu Pro Gly Phe Glu Gly Cys Ala Val Leu

100 105 110

Arg Ala Ser Leu Ala Thr Leu Ala Tyr Asp Arg Arg Asp Tyr Ala Gln

115 120 125

Leu Leu Asp Asp Thr Arg Cys Tyr Ser Ala Ala Leu Arg Ala Gly His

130 135 140

Ala Gln Ala Val Ala Ala Glu Arg Trp Ser Tyr Ala Glu Tyr Leu His

145 150 155 160

Asn Gly Ile Asp Ser Ile Ala Tyr Ala Asn Val Phe Cys Cys Leu Ser

165 170 175

Leu Leu Trp Gly Leu Asp Met Ala Thr Leu Arg Ala Arg Pro Ala Phe

180 185 190

Arg Gln Val Leu Arg Leu Ile Ser Ala Ile Gly Arg Leu Gln Asn Asp

195 200 205

Leu His Gly Cys Asp Lys Asp Arg Ser Ala Gly Glu Ala Asp Asn Ala

210 215 220

Val Ile Leu Leu Leu Gln Arg Tyr Pro Ala Met Pro Val Val Glu Phe

225 230 235 240

Leu Asn Asp Glu Leu Ala Gly His Thr Arg Met Leu His Arg Val Met

245 250 255

Ala Glu Glu Arg Phe Pro Ala Pro Trp Gly Pro Leu Ile Glu Ala Met

260 265 270

Ala Ala Ile Arg Val Gln Tyr Tyr Arg Thr Ser Thr Ser Arg Tyr Arg

275 280 285

Ser Asp Ala Val Arg Gly Gly Gln Arg Ala Pro Ala

290 295 300

<210> 17

<211> 2838

<212> DNA

<213> Phaeosphaeria sp.

<220>

<221> CDS

<222> (1)..(2838)

<400> 17

atg ttt gct aag ttt gat atg ttg gaa gaa gaa gct aga gct ttg gtc 48

Met Phe Ala Lys Phe Asp Met Leu Glu Glu Glu Ala Arg Ala Leu Val

1 5 10 15

aga aag gtc ggt aac gct gtt gac cca atc tac ggt ttc tct acc act 96

Arg Lys Val Gly Asn Ala Val Asp Pro Ile Tyr Gly Phe Ser Thr Thr

20 25 30

tcc tgt caa atc tac gac acc gct tgg gct gct atg atc tcc aag gaa 144

Ser Cys Gln Ile Tyr Asp Thr Ala Trp Ala Ala Met Ile Ser Lys Glu

35 40 45

gaa cac ggt gac aag gtc tgg ttg ttc cca gaa tct ttc aaa tac ttg 192

Glu His Gly Asp Lys Val Trp Leu Phe Pro Glu Ser Phe Lys Tyr Leu

50 55 60

ttg gaa aag caa ggt gaa gat ggt tcc tgg gaa aga cat cca aga tcc 240

Leu Glu Lys Gln Gly Glu Asp Gly Ser Trp Glu Arg His Pro Arg Ser

65 70 75 80

aag act gtc ggt gtt ttg aac act gct gcc gct tgt ttg gcc ttg ttg 288

Lys Thr Val Gly Val Leu Asn Thr Ala Ala Ala Cys Leu Ala Leu Leu

85 90 95

aga cac gtc aag aac cct ttg caa ttg caa gac att gct gcc caa gac 336

Arg His Val Lys Asn Pro Leu Gln Leu Gln Asp Ile Ala Ala Gln Asp

100 105 110

atc gaa ttg aga atc caa aga ggt ttg cgt tct ttg gaa gaa caa ttg 384

Ile Glu Leu Arg Ile Gln Arg Gly Leu Arg Ser Leu Glu Glu Gln Leu

115 120 125

att gct tgg gat gat gtc ttg gac acc aac cac atc ggt gtc gaa atg 432

Ile Ala Trp Asp Asp Val Leu Asp Thr Asn His Ile Gly Val Glu Met

130 135 140

att gtt cca gct ttg ttg gac tac ttg caa gcc gaa gat gaa aac gtt 480

Ile Val Pro Ala Leu Leu Asp Tyr Leu Gln Ala Glu Asp Glu Asn Val

145 150 155 160

gac ttt gaa ttc gaa tcc cac tct cta ttg atg caa atg tac aaa gaa 528

Asp Phe Glu Phe Glu Ser His Ser Leu Leu Met Gln Met Tyr Lys Glu

165 170 175

aag atg gcc aga ttc tcc cca gaa tcc tta tac aga gct aga cca tct 576

Lys Met Ala Arg Phe Ser Pro Glu Ser Leu Tyr Arg Ala Arg Pro Ser

180 185 190

tct gcc tta cat aat ttg gaa gct ttg atc ggt aaa ttg gac ttt gac 624

Ser Ala Leu His Asn Leu Glu Ala Leu Ile Gly Lys Leu Asp Phe Asp

195 200 205

aag gtt ggt cac cac ttg tac aac ggt tcc atg atg gct tcc cca tct 672

Lys Val Gly His His Leu Tyr Asn Gly Ser Met Met Ala Ser Pro Ser

210 215 220

tcc act gct gcc ttt cta atg cat gcc tct cca tgg tcc cac gaa gct 720

Ser Thr Ala Ala Phe Leu Met His Ala Ser Pro Trp Ser His Glu Ala

225 230 235 240

gaa gct tac cta cgt cac gtt ttc gaa gct ggt act ggt aag ggt tcc 768

Glu Ala Tyr Leu Arg His Val Phe Glu Ala Gly Thr Gly Lys Gly Ser

245 250 255

ggt ggt ttc cca ggt act tac cca acc act tac ttc gaa ttg aac tgg 816

Gly Gly Phe Pro Gly Thr Tyr Pro Thr Thr Tyr Phe Glu Leu Asn Trp

260 265 270

gtt ttg tcc act ttg atg aaa tct ggt ttc act tta tct gat ttg gaa 864

Val Leu Ser Thr Leu Met Lys Ser Gly Phe Thr Leu Ser Asp Leu Glu

275 280 285

tgt gat gaa tta tct tcc att gcc aac acc att gct gaa ggt ttc gaa 912

Cys Asp Glu Leu Ser Ser Ile Ala Asn Thr Ile Ala Glu Gly Phe Glu

290 295 300

tgt gac cac ggt gtc atc ggt ttt gct cca aga gct gtc gat gtc gac 960

Cys Asp His Gly Val Ile Gly Phe Ala Pro Arg Ala Val Asp Val Asp

305 310 315 320

gac acc gct aag ggt ttg ttg act tta act tta tta ggt atg gat gaa 1008

Asp Thr Ala Lys Gly Leu Leu Thr Leu Thr Leu Leu Gly Met Asp Glu

325 330 335

ggt gtt tct cca gct cca atg att gct atg ttc gaa gcc aag gac cac 1056

Gly Val Ser Pro Ala Pro Met Ile Ala Met Phe Glu Ala Lys Asp His

340 345 350

ttc ttg act ttc ttg ggt gaa cgt gac cca tct ttc act tct aac tgt 1104

Phe Leu Thr Phe Leu Gly Glu Arg Asp Pro Ser Phe Thr Ser Asn Cys

355 360 365

cac gtt ttg tta tct ttg ttg cac aga acc gat ttg ttg caa tat ttg 1152

His Val Leu Leu Ser Leu Leu His Arg Thr Asp Leu Leu Gln Tyr Leu

370 375 380

cct caa atc aga aag acc acc acc ttc ttg tgt gaa gcc tgg tgg gct 1200

Pro Gln Ile Arg Lys Thr Thr Thr Phe Leu Cys Glu Ala Trp Trp Ala

385 390 395 400

tgt gac ggt caa atc aag gac aaa tgg cac tta tct cac ttg tac cca 1248

Cys Asp Gly Gln Ile Lys Asp Lys Trp His Leu Ser His Leu Tyr Pro

405 410 415

acc atg ttg atg gtc caa gct ttc gct gaa atc tta ttg aag tct gct 1296

Thr Met Leu Met Val Gln Ala Phe Ala Glu Ile Leu Leu Lys Ser Ala

420 425 430

gaa ggt gaa cca ttg cac gat gct ttc gat gcc gct act ttg tcc aga 1344

Glu Gly Glu Pro Leu His Asp Ala Phe Asp Ala Ala Thr Leu Ser Arg

435 440 445

gtc tcc att tgt gtt ttc caa gct tgt ttg aga act cta ttg gcc caa 1392

Val Ser Ile Cys Val Phe Gln Ala Cys Leu Arg Thr Leu Leu Ala Gln

450 455 460

tct caa gat ggt tct tgg cac ggt caa cca gaa gct tct tgt tac gct 1440

Ser Gln Asp Gly Ser Trp His Gly Gln Pro Glu Ala Ser Cys Tyr Ala

465 470 475 480

gtc ttg act ttg gct gaa tct ggt cgt ttg gtt ttg ttg caa gct ttg 1488

Val Leu Thr Leu Ala Glu Ser Gly Arg Leu Val Leu Leu Gln Ala Leu

485 490 495

caa cca caa atc gct gct gcc atg gaa aag gcc gct gat gtt atg caa 1536

Gln Pro Gln Ile Ala Ala Ala Met Glu Lys Ala Ala Asp Val Met Gln

500 505 510

gct ggt aga tgg tcc tgt tct gat cat gac tgt gac tgg acc tcc aag 1584

Ala Gly Arg Trp Ser Cys Ser Asp His Asp Cys Asp Trp Thr Ser Lys

515 520 525

acc gct tac aga gtt gac tta gtt gct gct gct tac aga tta gct gct 1632

Thr Ala Tyr Arg Val Asp Leu Val Ala Ala Ala Tyr Arg Leu Ala Ala

530 535 540

atg aaa gct tct tcc aac ttg act ttc acc gtc gat gac aac gtt tcc 1680

Met Lys Ala Ser Ser Asn Leu Thr Phe Thr Val Asp Asp Asn Val Ser

545 550 555 560

aag aga tcc aac ggt ttc caa caa ttg gtc ggt aga act gac ttg ttc 1728

Lys Arg Ser Asn Gly Phe Gln Gln Leu Val Gly Arg Thr Asp Leu Phe

565 570 575

tct ggt gtc cca gcc tgg gaa tta caa gct tct ttc ttg gaa tct gct 1776

Ser Gly Val Pro Ala Trp Glu Leu Gln Ala Ser Phe Leu Glu Ser Ala

580 585 590

ttg ttc gtt cct ttg ttg aga aac cat aga ttg gat gtt ttc gac aga 1824

Leu Phe Val Pro Leu Leu Arg Asn His Arg Leu Asp Val Phe Asp Arg

595 600 605

gat gac atc aag gtt tct aag gac cac tac ttg gac atg atc cca ttc 1872

Asp Asp Ile Lys Val Ser Lys Asp His Tyr Leu Asp Met Ile Pro Phe

610 615 620

acc tgg gtt ggt tgt aac aac aga tct aga acc tac gtt tct acc tct 1920

Thr Trp Val Gly Cys Asn Asn Arg Ser Arg Thr Tyr Val Ser Thr Ser

625 630 635 640

ttc ttg ttc gac atg atg atc atc tcc atg ttg ggt tac caa atc gat 1968

Phe Leu Phe Asp Met Met Ile Ile Ser Met Leu Gly Tyr Gln Ile Asp

645 650 655

gaa ttc ttc gaa gct gaa gct gct cca gct ttc gct caa tgt att ggt 2016

Glu Phe Phe Glu Ala Glu Ala Ala Pro Ala Phe Ala Gln Cys Ile Gly

660 665 670

caa ttg cac caa gtc gtc gac aag gtt gtt gat gaa gtc att gac gaa 2064

Gln Leu His Gln Val Val Asp Lys Val Val Asp Glu Val Ile Asp Glu

675 680 685

gtc gtc gat aag gtt gtc ggt aag gtc gtt ggt aag gtt gtt ggt aag 2112

Val Val Asp Lys Val Val Gly Lys Val Val Gly Lys Val Val Gly Lys

690 695 700

gtt gtc gac gaa cgt gtt gac tct cca acc cac gaa gcc att gcc atc 2160

Val Val Asp Glu Arg Val Asp Ser Pro Thr His Glu Ala Ile Ala Ile

705 710 715 720

tgt aac atc gaa gct tct tta aga aga ttc gtc gat cac gtt ttg cac 2208

Cys Asn Ile Glu Ala Ser Leu Arg Arg Phe Val Asp His Val Leu His

725 730 735

cac caa cat gtc tta cac gct tct caa caa gaa caa gac att ttg tgg 2256

His Gln His Val Leu His Ala Ser Gln Gln Glu Gln Asp Ile Leu Trp

740 745 750

aga gaa ttg aga gcc ttc ttg cac gct cac gtt gtc caa atg gcc gac 2304

Arg Glu Leu Arg Ala Phe Leu His Ala His Val Val Gln Met Ala Asp

755 760 765

aac tcc act ttg gct cca cca ggt aga act ttc ttc gac tgg gtt aga 2352

Asn Ser Thr Leu Ala Pro Pro Gly Arg Thr Phe Phe Asp Trp Val Arg

770 775 780

acc act gct gct gac cac gtt gcc tgt gct tac tct ttc gct ttc gct 2400

Thr Thr Ala Ala Asp His Val Ala Cys Ala Tyr Ser Phe Ala Phe Ala

785 790 795 800

tgt tgt atc acc tct gct act atc ggt caa ggt caa tct atg ttc gct 2448

Cys Cys Ile Thr Ser Ala Thr Ile Gly Gln Gly Gln Ser Met Phe Ala

805 810 815

acc gtc aac gaa ttg tac ttg gtc caa gct gct gct cgt cac atg acc 2496

Thr Val Asn Glu Leu Tyr Leu Val Gln Ala Ala Ala Arg His Met Thr

820 825 830

acc atg tgt aga atg tgt aac gac att ggt tcc gtc gac aga gat ttc 2544

Thr Met Cys Arg Met Cys Asn Asp Ile Gly Ser Val Asp Arg Asp Phe

835 840 845

atc gaa gct aac att aac tcc gtt cac ttc cca gaa ttc tcc act ttg 2592

Ile Glu Ala Asn Ile Asn Ser Val His Phe Pro Glu Phe Ser Thr Leu

850 855 860

tct ttg gtt gct gac aag aag aaa gct ttg gcc aga ttg gct gcc tac 2640

Ser Leu Val Ala Asp Lys Lys Lys Ala Leu Ala Arg Leu Ala Ala Tyr

865 870 875 880

gaa aag tct tgt ttg act cac act ttg gac caa ttt gaa aat gaa gtt 2688

Glu Lys Ser Cys Leu Thr His Thr Leu Asp Gln Phe Glu Asn Glu Val

885 890 895

tta caa tct cca cgt gtt tct tct gct gcc tcc ggt gat ttc aga acc 2736

Leu Gln Ser Pro Arg Val Ser Ser Ala Ala Ser Gly Asp Phe Arg Thr

900 905 910

aga aag gtt gcc gtt gtt aga ttc ttt gct gat gtc act gac ttc tac 2784

Arg Lys Val Ala Val Val Arg Phe Phe Ala Asp Val Thr Asp Phe Tyr

915 920 925

gat caa ttg tac att ttg aga gat ttg tcc tct tct ttg aag cat gtc 2832

Asp Gln Leu Tyr Ile Leu Arg Asp Leu Ser Ser Ser Leu Lys His Val

930 935 940

gga acc 2838

Gly Thr

945

<210> 18

<211> 946

<212> PRT

<213> Phaeosphaeria sp.

<400> 18

Met Phe Ala Lys Phe Asp Met Leu Glu Glu Glu Ala Arg Ala Leu Val

1 5 10 15

Arg Lys Val Gly Asn Ala Val Asp Pro Ile Tyr Gly Phe Ser Thr Thr

20 25 30

Ser Cys Gln Ile Tyr Asp Thr Ala Trp Ala Ala Met Ile Ser Lys Glu

35 40 45

Glu His Gly Asp Lys Val Trp Leu Phe Pro Glu Ser Phe Lys Tyr Leu

50 55 60

Leu Glu Lys Gln Gly Glu Asp Gly Ser Trp Glu Arg His Pro Arg Ser

65 70 75 80

Lys Thr Val Gly Val Leu Asn Thr Ala Ala Ala Cys Leu Ala Leu Leu

85 90 95

Arg His Val Lys Asn Pro Leu Gln Leu Gln Asp Ile Ala Ala Gln Asp

100 105 110

Ile Glu Leu Arg Ile Gln Arg Gly Leu Arg Ser Leu Glu Glu Gln Leu

115 120 125

Ile Ala Trp Asp Asp Val Leu Asp Thr Asn His Ile Gly Val Glu Met

130 135 140

Ile Val Pro Ala Leu Leu Asp Tyr Leu Gln Ala Glu Asp Glu Asn Val

145 150 155 160

Asp Phe Glu Phe Glu Ser His Ser Leu Leu Met Gln Met Tyr Lys Glu

165 170 175

Lys Met Ala Arg Phe Ser Pro Glu Ser Leu Tyr Arg Ala Arg Pro Ser

180 185 190

Ser Ala Leu His Asn Leu Glu Ala Leu Ile Gly Lys Leu Asp Phe Asp

195 200 205

Lys Val Gly His His Leu Tyr Asn Gly Ser Met Met Ala Ser Pro Ser

210 215 220

Ser Thr Ala Ala Phe Leu Met His Ala Ser Pro Trp Ser His Glu Ala

225 230 235 240

Glu Ala Tyr Leu Arg His Val Phe Glu Ala Gly Thr Gly Lys Gly Ser

245 250 255

Gly Gly Phe Pro Gly Thr Tyr Pro Thr Thr Tyr Phe Glu Leu Asn Trp

260 265 270

Val Leu Ser Thr Leu Met Lys Ser Gly Phe Thr Leu Ser Asp Leu Glu

275 280 285

Cys Asp Glu Leu Ser Ser Ile Ala Asn Thr Ile Ala Glu Gly Phe Glu

290 295 300

Cys Asp His Gly Val Ile Gly Phe Ala Pro Arg Ala Val Asp Val Asp

305 310 315 320

Asp Thr Ala Lys Gly Leu Leu Thr Leu Thr Leu Leu Gly Met Asp Glu

325 330 335

Gly Val Ser Pro Ala Pro Met Ile Ala Met Phe Glu Ala Lys Asp His

340 345 350

Phe Leu Thr Phe Leu Gly Glu Arg Asp Pro Ser Phe Thr Ser Asn Cys

355 360 365

His Val Leu Leu Ser Leu Leu His Arg Thr Asp Leu Leu Gln Tyr Leu

370 375 380

Pro Gln Ile Arg Lys Thr Thr Thr Phe Leu Cys Glu Ala Trp Trp Ala

385 390 395 400

Cys Asp Gly Gln Ile Lys Asp Lys Trp His Leu Ser His Leu Tyr Pro

405 410 415

Thr Met Leu Met Val Gln Ala Phe Ala Glu Ile Leu Leu Lys Ser Ala

420 425 430

Glu Gly Glu Pro Leu His Asp Ala Phe Asp Ala Ala Thr Leu Ser Arg

435 440 445

Val Ser Ile Cys Val Phe Gln Ala Cys Leu Arg Thr Leu Leu Ala Gln

450 455 460

Ser Gln Asp Gly Ser Trp His Gly Gln Pro Glu Ala Ser Cys Tyr Ala

465 470 475 480

Val Leu Thr Leu Ala Glu Ser Gly Arg Leu Val Leu Leu Gln Ala Leu

485 490 495

Gln Pro Gln Ile Ala Ala Ala Met Glu Lys Ala Ala Asp Val Met Gln

500 505 510

Ala Gly Arg Trp Ser Cys Ser Asp His Asp Cys Asp Trp Thr Ser Lys

515 520 525

Thr Ala Tyr Arg Val Asp Leu Val Ala Ala Ala Tyr Arg Leu Ala Ala

530 535 540

Met Lys Ala Ser Ser Asn Leu Thr Phe Thr Val Asp Asp Asn Val Ser

545 550 555 560

Lys Arg Ser Asn Gly Phe Gln Gln Leu Val Gly Arg Thr Asp Leu Phe

565 570 575

Ser Gly Val Pro Ala Trp Glu Leu Gln Ala Ser Phe Leu Glu Ser Ala

580 585 590

Leu Phe Val Pro Leu Leu Arg Asn His Arg Leu Asp Val Phe Asp Arg

595 600 605

Asp Asp Ile Lys Val Ser Lys Asp His Tyr Leu Asp Met Ile Pro Phe

610 615 620

Thr Trp Val Gly Cys Asn Asn Arg Ser Arg Thr Tyr Val Ser Thr Ser

625 630 635 640

Phe Leu Phe Asp Met Met Ile Ile Ser Met Leu Gly Tyr Gln Ile Asp

645 650 655

Glu Phe Phe Glu Ala Glu Ala Ala Pro Ala Phe Ala Gln Cys Ile Gly

660 665 670

Gln Leu His Gln Val Val Asp Lys Val Val Asp Glu Val Ile Asp Glu

675 680 685

Val Val Asp Lys Val Val Gly Lys Val Val Gly Lys Val Val Gly Lys

690 695 700

Val Val Asp Glu Arg Val Asp Ser Pro Thr His Glu Ala Ile Ala Ile

705 710 715 720

Cys Asn Ile Glu Ala Ser Leu Arg Arg Phe Val Asp His Val Leu His

725 730 735

His Gln His Val Leu His Ala Ser Gln Gln Glu Gln Asp Ile Leu Trp

740 745 750

Arg Glu Leu Arg Ala Phe Leu His Ala His Val Val Gln Met Ala Asp

755 760 765

Asn Ser Thr Leu Ala Pro Pro Gly Arg Thr Phe Phe Asp Trp Val Arg

770 775 780

Thr Thr Ala Ala Asp His Val Ala Cys Ala Tyr Ser Phe Ala Phe Ala

785 790 795 800

Cys Cys Ile Thr Ser Ala Thr Ile Gly Gln Gly Gln Ser Met Phe Ala

805 810 815

Thr Val Asn Glu Leu Tyr Leu Val Gln Ala Ala Ala Arg His Met Thr

820 825 830

Thr Met Cys Arg Met Cys Asn Asp Ile Gly Ser Val Asp Arg Asp Phe

835 840 845

Ile Glu Ala Asn Ile Asn Ser Val His Phe Pro Glu Phe Ser Thr Leu

850 855 860

Ser Leu Val Ala Asp Lys Lys Lys Ala Leu Ala Arg Leu Ala Ala Tyr

865 870 875 880

Glu Lys Ser Cys Leu Thr His Thr Leu Asp Gln Phe Glu Asn Glu Val

885 890 895

Leu Gln Ser Pro Arg Val Ser Ser Ala Ala Ser Gly Asp Phe Arg Thr

900 905 910

Arg Lys Val Ala Val Val Arg Phe Phe Ala Asp Val Thr Asp Phe Tyr

915 920 925

Asp Gln Leu Tyr Ile Leu Arg Asp Leu Ser Ser Ser Leu Lys His Val

930 935 940

Gly Thr

945

<210> 19

<211> 2856

<212> DNA

<213> Gibberella fujikuroi

<220>

<221> CDS

<222> (1)..(2856)

<400> 19

atg cca ggt aag atc gaa aac ggt act cca aag gat tta aag act ggt 48

Met Pro Gly Lys Ile Glu Asn Gly Thr Pro Lys Asp Leu Lys Thr Gly

1 5 10 15

aac gat ttc gtt tct gct gcc aag tct ttg ttg gac aga gct ttc aaa 96

Asn Asp Phe Val Ser Ala Ala Lys Ser Leu Leu Asp Arg Ala Phe Lys

20 25 30

tct cac cac tct tac tac ggt tta tgt tcc act tct tgt caa gtt tac 144

Ser His His Ser Tyr Tyr Gly Leu Cys Ser Thr Ser Cys Gln Val Tyr

35 40 45

gac act gct tgg gtt gcc atg atc cca aag acc aga gat aat gtc aaa 192

Asp Thr Ala Trp Val Ala Met Ile Pro Lys Thr Arg Asp Asn Val Lys

50 55 60

caa tgg ttg ttc cct gaa tgt ttc cac tac ttg ttg aaa acc caa gct 240

Gln Trp Leu Phe Pro Glu Cys Phe His Tyr Leu Leu Lys Thr Gln Ala

65 70 75 80

gct gat ggt tcc tgg ggt tct ttg cca acc act caa acc gct ggt atc 288

Ala Asp Gly Ser Trp Gly Ser Leu Pro Thr Thr Gln Thr Ala Gly Ile

85 90 95

ttg gat acc gct tcc gct gtc ttg gct ttg cta tgt cac gct caa gaa 336

Leu Asp Thr Ala Ser Ala Val Leu Ala Leu Leu Cys His Ala Gln Glu

100 105 110

cca ttg caa atc ttg gat gtt tct cca gat gaa atg ggt ttg aga atc 384

Pro Leu Gln Ile Leu Asp Val Ser Pro Asp Glu Met Gly Leu Arg Ile

115 120 125

gaa cac ggt gtt act tct ttg aag aga caa ttg gct gtc tgg aac gac 432

Glu His Gly Val Thr Ser Leu Lys Arg Gln Leu Ala Val Trp Asn Asp

130 135 140

gtc gag gac act aac cac att ggt gtc gaa ttt att att cca gct tta 480

Val Glu Asp Thr Asn His Ile Gly Val Glu Phe Ile Ile Pro Ala Leu

145 150 155 160

ttg tcc atg ttg gaa aag gaa ttg gat gtt cca tct ttc gaa ttc cca 528

Leu Ser Met Leu Glu Lys Glu Leu Asp Val Pro Ser Phe Glu Phe Pro

165 170 175

tgt aga tcc atc ttg gaa aga atg cac ggt gaa aag ttg ggt cac ttc 576

Cys Arg Ser Ile Leu Glu Arg Met His Gly Glu Lys Leu Gly His Phe

180 185 190

gat ttg gaa caa gtc tac ggt aag cca tct tct tta cta cat tct ttg 624

Asp Leu Glu Gln Val Tyr Gly Lys Pro Ser Ser Leu Leu His Ser Leu

195 200 205

gaa gct ttc tta ggt aag tta gac ttc gac aga ttg tcc cac cac ttg 672

Glu Ala Phe Leu Gly Lys Leu Asp Phe Asp Arg Leu Ser His His Leu

210 215 220

tac cac ggt tcc atg atg gct tct cca tcc tcc act gct gcc tac ttg 720

Tyr His Gly Ser Met Met Ala Ser Pro Ser Ser Thr Ala Ala Tyr Leu

225 230 235 240

att ggt gct acc aaa tgg gat gac gaa gct gag gac tac ttg aga cac 768

Ile Gly Ala Thr Lys Trp Asp Asp Glu Ala Glu Asp Tyr Leu Arg His

245 250 255

gtt atg aga aac ggt gct ggt cat ggt aac ggt ggt atc tcc ggt act 816

Val Met Arg Asn Gly Ala Gly His Gly Asn Gly Gly Ile Ser Gly Thr

260 265 270

ttc cca acc acc cat ttc gaa tgt tcc tgg atc att gcc act ttg ttg 864

Phe Pro Thr Thr His Phe Glu Cys Ser Trp Ile Ile Ala Thr Leu Leu

275 280 285

aag gtt ggt ttc acc ttg aaa caa att gac ggt gac ggt ttg aga ggt 912

Lys Val Gly Phe Thr Leu Lys Gln Ile Asp Gly Asp Gly Leu Arg Gly

290 295 300

cta tcc act atc tta ttg gaa gct tta cgt gac gaa aac ggt gtc atc 960

Leu Ser Thr Ile Leu Leu Glu Ala Leu Arg Asp Glu Asn Gly Val Ile

305 310 315 320

ggt ttc gct cca aga acc gct gat gtc gac gac acc gcc aag gct ttg 1008

Gly Phe Ala Pro Arg Thr Ala Asp Val Asp Asp Thr Ala Lys Ala Leu

325 330 335

ttg gct ttg tct tta gtt aac caa cct gtc tct cca gat atc atg att 1056

Leu Ala Leu Ser Leu Val Asn Gln Pro Val Ser Pro Asp Ile Met Ile

340 345 350

aag gtt ttc gaa ggt aag gac cat ttt acc act ttc ggt tct gaa cgt 1104

Lys Val Phe Glu Gly Lys Asp His Phe Thr Thr Phe Gly Ser Glu Arg

355 360 365

gat cca tct ttg acc tct aac ttg cac gtc ttg ttg tct ttg ttg aag 1152

Asp Pro Ser Leu Thr Ser Asn Leu His Val Leu Leu Ser Leu Leu Lys

370 375 380

caa tct aac ttg tct caa tac cat cca caa atc tta aag act act ttg 1200

Gln Ser Asn Leu Ser Gln Tyr His Pro Gln Ile Leu Lys Thr Thr Leu

385 390 395 400

ttt acc tgt aga tgg tgg tgg ggt tct gac cat tgt gtc aag gac aag 1248

Phe Thr Cys Arg Trp Trp Trp Gly Ser Asp His Cys Val Lys Asp Lys

405 410 415

tgg aac ttg tct cat ttg tac cca acc atg ttg ttg gtc gaa gcc ttc 1296

Trp Asn Leu Ser His Leu Tyr Pro Thr Met Leu Leu Val Glu Ala Phe

420 425 430

act gaa gtc ttg cac ttg att gac ggt ggt gaa ttg tcc tct ttg ttc 1344

Thr Glu Val Leu His Leu Ile Asp Gly Gly Glu Leu Ser Ser Leu Phe

435 440 445

gat gaa tct ttc aaa tgt aag atc ggt ttg tcc att ttc caa gct gtt 1392

Asp Glu Ser Phe Lys Cys Lys Ile Gly Leu Ser Ile Phe Gln Ala Val

450 455 460

ttg aga atc atc ttg act caa gac aac gat ggt tcc tgg aga ggt tac 1440

Leu Arg Ile Ile Leu Thr Gln Asp Asn Asp Gly Ser Trp Arg Gly Tyr

465 470 475 480

aga gaa caa acc tgt tac gcc att ttg gct ttg gtc caa gct cgt cac 1488

Arg Glu Gln Thr Cys Tyr Ala Ile Leu Ala Leu Val Gln Ala Arg His

485 490 495

gtt tgt ttc ttc act cac atg gtc gat aga tta caa tct tgt gtc gac 1536

Val Cys Phe Phe Thr His Met Val Asp Arg Leu Gln Ser Cys Val Asp

500 505 510

aga ggt ttc tcc tgg tta aag tct tgt tcc ttc cac tct caa gac ttg 1584

Arg Gly Phe Ser Trp Leu Lys Ser Cys Ser Phe His Ser Gln Asp Leu

515 520 525

act tgg acc tcc aag act gct tac gaa gtt ggt ttc gtt gct gaa gcc 1632

Thr Trp Thr Ser Lys Thr Ala Tyr Glu Val Gly Phe Val Ala Glu Ala

530 535 540

tac aaa ttg gct gct ttg caa tct gct tct ttg gaa gtt cca gct gct 1680

Tyr Lys Leu Ala Ala Leu Gln Ser Ala Ser Leu Glu Val Pro Ala Ala

545 550 555 560

acc atc ggt cac tcc gtt acc tct gct gtc cca tct tct gac ttg gaa 1728

Thr Ile Gly His Ser Val Thr Ser Ala Val Pro Ser Ser Asp Leu Glu

565 570 575

aag tac atg aga ttg gtc cgt aag act gct ttg ttc tct cca ttg gac 1776

Lys Tyr Met Arg Leu Val Arg Lys Thr Ala Leu Phe Ser Pro Leu Asp

580 585 590

gaa tgg ggt ttg atg gct tcc att att gaa tct tct ttc ttt gtc cca 1824

Glu Trp Gly Leu Met Ala Ser Ile Ile Glu Ser Ser Phe Phe Val Pro

595 600 605

ttg ttg caa gct caa aga gtc gaa atc tac cca aga gat aat atc aag 1872

Leu Leu Gln Ala Gln Arg Val Glu Ile Tyr Pro Arg Asp Asn Ile Lys

610 615 620

gtt gac gag gac aag tac ttg tcc atc att cca ttt acc tgg gtt ggt 1920

Val Asp Glu Asp Lys Tyr Leu Ser Ile Ile Pro Phe Thr Trp Val Gly

625 630 635 640

tgt aat aac aga tct aga act ttc gct tct aac aga tgg tta tat gac 1968

Cys Asn Asn Arg Ser Arg Thr Phe Ala Ser Asn Arg Trp Leu Tyr Asp

645 650 655

atg atg tac tta tct ttg ttg ggt tac caa act gac gaa tac atg gaa 2016

Met Met Tyr Leu Ser Leu Leu Gly Tyr Gln Thr Asp Glu Tyr Met Glu

660 665 670

gct gtt gcc ggt cca gtt ttc ggt gat gtt tct cta ttg cac caa acc 2064

Ala Val Ala Gly Pro Val Phe Gly Asp Val Ser Leu Leu His Gln Thr

675 680 685

atc gac aag gtt atc gac aac acc atg ggt aac ttg gcc aga gcc aac 2112

Ile Asp Lys Val Ile Asp Asn Thr Met Gly Asn Leu Ala Arg Ala Asn

690 695 700

ggt act gtt cac tct ggt aac ggt cac caa cac gaa tct cca aac att 2160

Gly Thr Val His Ser Gly Asn Gly His Gln His Glu Ser Pro Asn Ile

705 710 715 720

ggt caa gtt gag gac act ttg acc aga ttc acc aac tcc gtt ttg aac 2208

Gly Gln Val Glu Asp Thr Leu Thr Arg Phe Thr Asn Ser Val Leu Asn

725 730 735

cac aag gac gtc ttg aac tct tct tct tct gac caa gat acc tta cgt 2256

His Lys Asp Val Leu Asn Ser Ser Ser Ser Asp Gln Asp Thr Leu Arg

740 745 750

cgt gaa ttc aga act ttc atg cac gct cac atc acc caa atc gaa gat 2304

Arg Glu Phe Arg Thr Phe Met His Ala His Ile Thr Gln Ile Glu Asp

755 760 765

aac tcc aga ttc tcc aag caa gcc tcc tct gat gcc ttt tct tct cca 2352

Asn Ser Arg Phe Ser Lys Gln Ala Ser Ser Asp Ala Phe Ser Ser Pro

770 775 780

gaa caa tct tac ttc caa tgg gtt aac tcc act ggt ggt tct cac gtt 2400

Glu Gln Ser Tyr Phe Gln Trp Val Asn Ser Thr Gly Gly Ser His Val

785 790 795 800

gct tgt gcc tac tcc ttc gcc ttt tcc aac tgt ttg atg tcc gct aac 2448

Ala Cys Ala Tyr Ser Phe Ala Phe Ser Asn Cys Leu Met Ser Ala Asn

805 810 815

tta ttg caa ggt aag gat gct ttc cca tct ggt act caa aag tac ttg 2496

Leu Leu Gln Gly Lys Asp Ala Phe Pro Ser Gly Thr Gln Lys Tyr Leu

820 825 830

atc tcc tcc gtc atg aga cac gct acc aac atg tgt cgt atg tac aac 2544

Ile Ser Ser Val Met Arg His Ala Thr Asn Met Cys Arg Met Tyr Asn

835 840 845

gat ttc ggt tct att gcc aga gac aac gct gaa aga aac gtt aac tcc 2592

Asp Phe Gly Ser Ile Ala Arg Asp Asn Ala Glu Arg Asn Val Asn Ser

850 855 860

att cac ttc cct gaa ttc act cta tgt aac ggt act tct caa aac ttg 2640

Ile His Phe Pro Glu Phe Thr Leu Cys Asn Gly Thr Ser Gln Asn Leu

865 870 875 880

gat gaa aga aag gaa aga tta ttg aag atc gct acc tac gaa caa ggt 2688

Asp Glu Arg Lys Glu Arg Leu Leu Lys Ile Ala Thr Tyr Glu Gln Gly

885 890 895

tac ttg gac aga gct ttg gaa gct ttg gaa aga caa tcc aga gat gac 2736

Tyr Leu Asp Arg Ala Leu Glu Ala Leu Glu Arg Gln Ser Arg Asp Asp

900 905 910

gct ggt gac aga gct ggt tcc aag gac atg aga aaa ttg aag att gtc 2784

Ala Gly Asp Arg Ala Gly Ser Lys Asp Met Arg Lys Leu Lys Ile Val

915 920 925

aaa ttg ttc tgt gat gtt acc gac ttg tac gac caa ttg tac gtc atc 2832

Lys Leu Phe Cys Asp Val Thr Asp Leu Tyr Asp Gln Leu Tyr Val Ile

930 935 940

aag gac ttg tcc tct tcc atg aag 2856

Lys Asp Leu Ser Ser Ser Met Lys

945 950

<210> 20

<211> 952

<212> PRT

<213> Gibberella fujikuroi

<400> 20

Met Pro Gly Lys Ile Glu Asn Gly Thr Pro Lys Asp Leu Lys Thr Gly

1 5 10 15

Asn Asp Phe Val Ser Ala Ala Lys Ser Leu Leu Asp Arg Ala Phe Lys

20 25 30

Ser His His Ser Tyr Tyr Gly Leu Cys Ser Thr Ser Cys Gln Val Tyr

35 40 45

Asp Thr Ala Trp Val Ala Met Ile Pro Lys Thr Arg Asp Asn Val Lys

50 55 60

Gln Trp Leu Phe Pro Glu Cys Phe His Tyr Leu Leu Lys Thr Gln Ala

65 70 75 80

Ala Asp Gly Ser Trp Gly Ser Leu Pro Thr Thr Gln Thr Ala Gly Ile

85 90 95

Leu Asp Thr Ala Ser Ala Val Leu Ala Leu Leu Cys His Ala Gln Glu

100 105 110

Pro Leu Gln Ile Leu Asp Val Ser Pro Asp Glu Met Gly Leu Arg Ile

115 120 125

Glu His Gly Val Thr Ser Leu Lys Arg Gln Leu Ala Val Trp Asn Asp

130 135 140

Val Glu Asp Thr Asn His Ile Gly Val Glu Phe Ile Ile Pro Ala Leu

145 150 155 160

Leu Ser Met Leu Glu Lys Glu Leu Asp Val Pro Ser Phe Glu Phe Pro

165 170 175

Cys Arg Ser Ile Leu Glu Arg Met His Gly Glu Lys Leu Gly His Phe

180 185 190

Asp Leu Glu Gln Val Tyr Gly Lys Pro Ser Ser Leu Leu His Ser Leu

195 200 205

Glu Ala Phe Leu Gly Lys Leu Asp Phe Asp Arg Leu Ser His His Leu

210 215 220

Tyr His Gly Ser Met Met Ala Ser Pro Ser Ser Thr Ala Ala Tyr Leu

225 230 235 240

Ile Gly Ala Thr Lys Trp Asp Asp Glu Ala Glu Asp Tyr Leu Arg His

245 250 255

Val Met Arg Asn Gly Ala Gly His Gly Asn Gly Gly Ile Ser Gly Thr

260 265 270

Phe Pro Thr Thr His Phe Glu Cys Ser Trp Ile Ile Ala Thr Leu Leu

275 280 285

Lys Val Gly Phe Thr Leu Lys Gln Ile Asp Gly Asp Gly Leu Arg Gly

290 295 300

Leu Ser Thr Ile Leu Leu Glu Ala Leu Arg Asp Glu Asn Gly Val Ile

305 310 315 320

Gly Phe Ala Pro Arg Thr Ala Asp Val Asp Asp Thr Ala Lys Ala Leu

325 330 335

Leu Ala Leu Ser Leu Val Asn Gln Pro Val Ser Pro Asp Ile Met Ile

340 345 350

Lys Val Phe Glu Gly Lys Asp His Phe Thr Thr Phe Gly Ser Glu Arg

355 360 365

Asp Pro Ser Leu Thr Ser Asn Leu His Val Leu Leu Ser Leu Leu Lys

370 375 380

Gln Ser Asn Leu Ser Gln Tyr His Pro Gln Ile Leu Lys Thr Thr Leu

385 390 395 400

Phe Thr Cys Arg Trp Trp Trp Gly Ser Asp His Cys Val Lys Asp Lys

405 410 415

Trp Asn Leu Ser His Leu Tyr Pro Thr Met Leu Leu Val Glu Ala Phe

420 425 430

Thr Glu Val Leu His Leu Ile Asp Gly Gly Glu Leu Ser Ser Leu Phe

435 440 445

Asp Glu Ser Phe Lys Cys Lys Ile Gly Leu Ser Ile Phe Gln Ala Val

450 455 460

Leu Arg Ile Ile Leu Thr Gln Asp Asn Asp Gly Ser Trp Arg Gly Tyr

465 470 475 480

Arg Glu Gln Thr Cys Tyr Ala Ile Leu Ala Leu Val Gln Ala Arg His

485 490 495

Val Cys Phe Phe Thr His Met Val Asp Arg Leu Gln Ser Cys Val Asp

500 505 510

Arg Gly Phe Ser Trp Leu Lys Ser Cys Ser Phe His Ser Gln Asp Leu

515 520 525

Thr Trp Thr Ser Lys Thr Ala Tyr Glu Val Gly Phe Val Ala Glu Ala

530 535 540

Tyr Lys Leu Ala Ala Leu Gln Ser Ala Ser Leu Glu Val Pro Ala Ala

545 550 555 560

Thr Ile Gly His Ser Val Thr Ser Ala Val Pro Ser Ser Asp Leu Glu

565 570 575

Lys Tyr Met Arg Leu Val Arg Lys Thr Ala Leu Phe Ser Pro Leu Asp

580 585 590

Glu Trp Gly Leu Met Ala Ser Ile Ile Glu Ser Ser Phe Phe Val Pro

595 600 605

Leu Leu Gln Ala Gln Arg Val Glu Ile Tyr Pro Arg Asp Asn Ile Lys

610 615 620

Val Asp Glu Asp Lys Tyr Leu Ser Ile Ile Pro Phe Thr Trp Val Gly

625 630 635 640

Cys Asn Asn Arg Ser Arg Thr Phe Ala Ser Asn Arg Trp Leu Tyr Asp

645 650 655

Met Met Tyr Leu Ser Leu Leu Gly Tyr Gln Thr Asp Glu Tyr Met Glu

660 665 670

Ala Val Ala Gly Pro Val Phe Gly Asp Val Ser Leu Leu His Gln Thr

675 680 685

Ile Asp Lys Val Ile Asp Asn Thr Met Gly Asn Leu Ala Arg Ala Asn

690 695 700

Gly Thr Val His Ser Gly Asn Gly His Gln His Glu Ser Pro Asn Ile

705 710 715 720

Gly Gln Val Glu Asp Thr Leu Thr Arg Phe Thr Asn Ser Val Leu Asn

725 730 735

His Lys Asp Val Leu Asn Ser Ser Ser Ser Asp Gln Asp Thr Leu Arg

740 745 750

Arg Glu Phe Arg Thr Phe Met His Ala His Ile Thr Gln Ile Glu Asp

755 760 765

Asn Ser Arg Phe Ser Lys Gln Ala Ser Ser Asp Ala Phe Ser Ser Pro

770 775 780

Glu Gln Ser Tyr Phe Gln Trp Val Asn Ser Thr Gly Gly Ser His Val

785 790 795 800

Ala Cys Ala Tyr Ser Phe Ala Phe Ser Asn Cys Leu Met Ser Ala Asn

805 810 815

Leu Leu Gln Gly Lys Asp Ala Phe Pro Ser Gly Thr Gln Lys Tyr Leu

820 825 830

Ile Ser Ser Val Met Arg His Ala Thr Asn Met Cys Arg Met Tyr Asn

835 840 845

Asp Phe Gly Ser Ile Ala Arg Asp Asn Ala Glu Arg Asn Val Asn Ser

850 855 860

Ile His Phe Pro Glu Phe Thr Leu Cys Asn Gly Thr Ser Gln Asn Leu

865 870 875 880

Asp Glu Arg Lys Glu Arg Leu Leu Lys Ile Ala Thr Tyr Glu Gln Gly

885 890 895

Tyr Leu Asp Arg Ala Leu Glu Ala Leu Glu Arg Gln Ser Arg Asp Asp

900 905 910

Ala Gly Asp Arg Ala Gly Ser Lys Asp Met Arg Lys Leu Lys Ile Val

915 920 925

Lys Leu Phe Cys Asp Val Thr Asp Leu Tyr Asp Gln Leu Tyr Val Ile

930 935 940

Lys Asp Leu Ser Ser Ser Met Lys

945 950

<210> 21

<211> 1533

<212> DNA

<213> Lactuca sativa

<220>

<221> CDS

<222> (1)..(1533)

<400> 21

atg gac ttg caa acc atg gcc cca atg ggt tct gct gct atc gcc atc 48

Met Asp Leu Gln Thr Met Ala Pro Met Gly Ser Ala Ala Ile Ala Ile

1 5 10 15

ggt ggt cca gct gtc gcc gtt gct ggt ggt atc tcc ttg ttg ttc ttg 96

Gly Gly Pro Ala Val Ala Val Ala Gly Gly Ile Ser Leu Leu Phe Leu

20 25 30

aaa tcc ttc ttg tct caa caa cca ggt aac cca aac cac ttg cca tct 144

Lys Ser Phe Leu Ser Gln Gln Pro Gly Asn Pro Asn His Leu Pro Ser

35 40 45

gtc cca gct gtc cca ggt gtt cct cta tta ggt aac ttg ttg gaa ttg 192

Val Pro Ala Val Pro Gly Val Pro Leu Leu Gly Asn Leu Leu Glu Leu

50 55 60

aag gaa aag aag cca tac aag acc ttt acc aaa tgg gct gaa act tac 240

Lys Glu Lys Lys Pro Tyr Lys Thr Phe Thr Lys Trp Ala Glu Thr Tyr

65 70 75 80

ggt cca atc tac tcc atc aag acc ggt gct acc tcc atg gtt gtc gtt 288

Gly Pro Ile Tyr Ser Ile Lys Thr Gly Ala Thr Ser Met Val Val Val

85 90 95

aac tcc aac caa ttg gcc aag gaa gct atg gtc acc aga ttc gac tcc 336

Asn Ser Asn Gln Leu Ala Lys Glu Ala Met Val Thr Arg Phe Asp Ser

100 105 110

atc tct acc aga aag ttg tcc aag gct ttg caa att ttg act gct gac 384

Ile Ser Thr Arg Lys Leu Ser Lys Ala Leu Gln Ile Leu Thr Ala Asp

115 120 125

aag acc atg gtt gcc atg tct gac tac gat gac tac cat aaa act gtc 432

Lys Thr Met Val Ala Met Ser Asp Tyr Asp Asp Tyr His Lys Thr Val

130 135 140

aag aga aac ttg ttg act tct att ttg ggt cca gct gct caa aag aga 480

Lys Arg Asn Leu Leu Thr Ser Ile Leu Gly Pro Ala Ala Gln Lys Arg

145 150 155 160

cac aga gcc cac cgt gac gct atg ggt gac aac ttg tcc aga caa ttg 528

His Arg Ala His Arg Asp Ala Met Gly Asp Asn Leu Ser Arg Gln Leu

165 170 175

cac gct tta gct ttg aac tct cct caa gaa gcc att aac ttc aga caa 576

His Ala Leu Ala Leu Asn Ser Pro Gln Glu Ala Ile Asn Phe Arg Gln

180 185 190

atc ttc caa tct gaa tta ttc act tta gct ttc aag caa act ttc ggt 624

Ile Phe Gln Ser Glu Leu Phe Thr Leu Ala Phe Lys Gln Thr Phe Gly

195 200 205

aga gat atc gaa tct atc ttc gtc ggt gat ttg ggt act acc atg acc 672

Arg Asp Ile Glu Ser Ile Phe Val Gly Asp Leu Gly Thr Thr Met Thr

210 215 220

aga gaa gaa atg ttc caa att ttg gtt gtt gac cca atg atg ggt gct 720

Arg Glu Glu Met Phe Gln Ile Leu Val Val Asp Pro Met Met Gly Ala

225 230 235 240

att gac gtt gac tgg aga gac ttc ttc cca tac ttg aaa tgg att cca 768

Ile Asp Val Asp Trp Arg Asp Phe Phe Pro Tyr Leu Lys Trp Ile Pro

245 250 255

aac gcc aaa ttg gaa gaa aag atc gaa caa atg tac atc aga aga aag 816

Asn Ala Lys Leu Glu Glu Lys Ile Glu Gln Met Tyr Ile Arg Arg Lys

260 265 270

gct gtt atg aag gct gtt atc caa gaa cac aga aag aga att gat tct 864

Ala Val Met Lys Ala Val Ile Gln Glu His Arg Lys Arg Ile Asp Ser

275 280 285

ggt gaa aac ttg gat tct tac att gat ttc ttg ttg gct gaa gct caa 912

Gly Glu Asn Leu Asp Ser Tyr Ile Asp Phe Leu Leu Ala Glu Ala Gln

290 295 300

cca ttg act gaa aag caa tta ttg atg tcc ttg tgg gaa cca att att 960

Pro Leu Thr Glu Lys Gln Leu Leu Met Ser Leu Trp Glu Pro Ile Ile

305 310 315 320

gaa act tct gac acc acc atg gtt acc act gaa tgg gct atg tac gaa 1008

Glu Thr Ser Asp Thr Thr Met Val Thr Thr Glu Trp Ala Met Tyr Glu

325 330 335

ttg tct aag cac cca aac aag caa caa aga tta tac aat gaa atc aga 1056

Leu Ser Lys His Pro Asn Lys Gln Gln Arg Leu Tyr Asn Glu Ile Arg

340 345 350

aac atc tgt ggt tct gaa aag atc act gaa gaa aag ttg tgt aag atg 1104

Asn Ile Cys Gly Ser Glu Lys Ile Thr Glu Glu Lys Leu Cys Lys Met

355 360 365

cca tac tta tct gct gtc ttt cac gaa act tta cgt gtt cac tct cca 1152

Pro Tyr Leu Ser Ala Val Phe His Glu Thr Leu Arg Val His Ser Pro

370 375 380

gtt tcc atc att cca tta cgt tac gtt cac gaa aac act gaa ttg ggt 1200

Val Ser Ile Ile Pro Leu Arg Tyr Val His Glu Asn Thr Glu Leu Gly

385 390 395 400

ggt tac cat gtc cca gct ggt act gaa ttg gct gtc aac atc tac ggt 1248

Gly Tyr His Val Pro Ala Gly Thr Glu Leu Ala Val Asn Ile Tyr Gly

405 410 415

tgt aac atg gaa aga gaa atc tgg gaa aac cca gaa gaa tgg tcc cca 1296

Cys Asn Met Glu Arg Glu Ile Trp Glu Asn Pro Glu Glu Trp Ser Pro

420 425 430

gaa aga ttc ttg gct gaa aac gaa cca gtt aac ttg caa aag acc atg 1344

Glu Arg Phe Leu Ala Glu Asn Glu Pro Val Asn Leu Gln Lys Thr Met

435 440 445

gcc ttt ggt gct ggt aag aga gtc tgt gcc ggt gct atg caa gct atg 1392

Ala Phe Gly Ala Gly Lys Arg Val Cys Ala Gly Ala Met Gln Ala Met

450 455 460

ttg ttg gct tgt gtc ggt att ggt aga atg gtt caa gaa ttc gaa tgg 1440

Leu Leu Ala Cys Val Gly Ile Gly Arg Met Val Gln Glu Phe Glu Trp

465 470 475 480

aga ttg aaa gat gac gtc gaa gaa gat gtc aac act tta ggt ttg acc 1488

Arg Leu Lys Asp Asp Val Glu Glu Asp Val Asn Thr Leu Gly Leu Thr

485 490 495

act caa aga ttg aac cca atg ttg gct gtt atc aag cct agg aat 1533

Thr Gln Arg Leu Asn Pro Met Leu Ala Val Ile Lys Pro Arg Asn

500 505 510

<210> 22

<211> 511

<212> PRT

<213> Lactuca sativa

<400> 22

Met Asp Leu Gln Thr Met Ala Pro Met Gly Ser Ala Ala Ile Ala Ile

1 5 10 15

Gly Gly Pro Ala Val Ala Val Ala Gly Gly Ile Ser Leu Leu Phe Leu

20 25 30

Lys Ser Phe Leu Ser Gln Gln Pro Gly Asn Pro Asn His Leu Pro Ser

35 40 45

Val Pro Ala Val Pro Gly Val Pro Leu Leu Gly Asn Leu Leu Glu Leu

50 55 60

Lys Glu Lys Lys Pro Tyr Lys Thr Phe Thr Lys Trp Ala Glu Thr Tyr

65 70 75 80

Gly Pro Ile Tyr Ser Ile Lys Thr Gly Ala Thr Ser Met Val Val Val

85 90 95

Asn Ser Asn Gln Leu Ala Lys Glu Ala Met Val Thr Arg Phe Asp Ser

100 105 110

Ile Ser Thr Arg Lys Leu Ser Lys Ala Leu Gln Ile Leu Thr Ala Asp

115 120 125

Lys Thr Met Val Ala Met Ser Asp Tyr Asp Asp Tyr His Lys Thr Val

130 135 140

Lys Arg Asn Leu Leu Thr Ser Ile Leu Gly Pro Ala Ala Gln Lys Arg

145 150 155 160

His Arg Ala His Arg Asp Ala Met Gly Asp Asn Leu Ser Arg Gln Leu

165 170 175

His Ala Leu Ala Leu Asn Ser Pro Gln Glu Ala Ile Asn Phe Arg Gln

180 185 190

Ile Phe Gln Ser Glu Leu Phe Thr Leu Ala Phe Lys Gln Thr Phe Gly

195 200 205

Arg Asp Ile Glu Ser Ile Phe Val Gly Asp Leu Gly Thr Thr Met Thr

210 215 220

Arg Glu Glu Met Phe Gln Ile Leu Val Val Asp Pro Met Met Gly Ala

225 230 235 240

Ile Asp Val Asp Trp Arg Asp Phe Phe Pro Tyr Leu Lys Trp Ile Pro

245 250 255

Asn Ala Lys Leu Glu Glu Lys Ile Glu Gln Met Tyr Ile Arg Arg Lys

260 265 270

Ala Val Met Lys Ala Val Ile Gln Glu His Arg Lys Arg Ile Asp Ser

275 280 285

Gly Glu Asn Leu Asp Ser Tyr Ile Asp Phe Leu Leu Ala Glu Ala Gln

290 295 300

Pro Leu Thr Glu Lys Gln Leu Leu Met Ser Leu Trp Glu Pro Ile Ile

305 310 315 320

Glu Thr Ser Asp Thr Thr Met Val Thr Thr Glu Trp Ala Met Tyr Glu

325 330 335

Leu Ser Lys His Pro Asn Lys Gln Gln Arg Leu Tyr Asn Glu Ile Arg

340 345 350

Asn Ile Cys Gly Ser Glu Lys Ile Thr Glu Glu Lys Leu Cys Lys Met

355 360 365

Pro Tyr Leu Ser Ala Val Phe His Glu Thr Leu Arg Val His Ser Pro

370 375 380

Val Ser Ile Ile Pro Leu Arg Tyr Val His Glu Asn Thr Glu Leu Gly

385 390 395 400

Gly Tyr His Val Pro Ala Gly Thr Glu Leu Ala Val Asn Ile Tyr Gly

405 410 415

Cys Asn Met Glu Arg Glu Ile Trp Glu Asn Pro Glu Glu Trp Ser Pro

420 425 430

Glu Arg Phe Leu Ala Glu Asn Glu Pro Val Asn Leu Gln Lys Thr Met

435 440 445

Ala Phe Gly Ala Gly Lys Arg Val Cys Ala Gly Ala Met Gln Ala Met

450 455 460

Leu Leu Ala Cys Val Gly Ile Gly Arg Met Val Gln Glu Phe Glu Trp

465 470 475 480

Arg Leu Lys Asp Asp Val Glu Glu Asp Val Asn Thr Leu Gly Leu Thr

485 490 495

Thr Gln Arg Leu Asn Pro Met Leu Ala Val Ile Lys Pro Arg Asn

500 505 510

<210> 23

<211> 1536

<212> DNA

<213> Lactuca sativa

<220>

<221> CDS

<222> (1)..(1536)

<400> 23

atg gat ggt gtc att gac atg caa acc att cca ttg aga acc gcc att 48

Met Asp Gly Val Ile Asp Met Gln Thr Ile Pro Leu Arg Thr Ala Ile

1 5 10 15

gcc att ggt ggt act gct gtt gct ttg gtt gtt gct cta tac ttc tgg 96

Ala Ile Gly Gly Thr Ala Val Ala Leu Val Val Ala Leu Tyr Phe Trp

20 25 30

ttc ttg aga tct tac gct tct cca tct cac cac tct aac cat ttg cca 144

Phe Leu Arg Ser Tyr Ala Ser Pro Ser His His Ser Asn His Leu Pro

35 40 45

cct gtt cca gaa gtt cca ggt gtc cca gtc ttg ggt aac ttg ttg caa 192

Pro Val Pro Glu Val Pro Gly Val Pro Val Leu Gly Asn Leu Leu Gln

50 55 60

ttg aaa gaa aag aag cca tac atg act ttc acc aaa tgg gct gaa atg 240

Leu Lys Glu Lys Lys Pro Tyr Met Thr Phe Thr Lys Trp Ala Glu Met

65 70 75 80

tac ggt cca atc tac tct atc aga act ggt gct acc tcc atg gtt gtt 288

Tyr Gly Pro Ile Tyr Ser Ile Arg Thr Gly Ala Thr Ser Met Val Val

85 90 95

gtt tcc tct aac gaa att gcc aag gaa gtt gtt gtc act aga ttc cca 336

Val Ser Ser Asn Glu Ile Ala Lys Glu Val Val Val Thr Arg Phe Pro

100 105 110

tcc atc tcc acc aga aag ttg tct tac gct ttg aag gtc ttg act gaa 384

Ser Ile Ser Thr Arg Lys Leu Ser Tyr Ala Leu Lys Val Leu Thr Glu

115 120 125

gat aag tcc atg gtt gct atg tct gat tac cat gac tac cac aag acc 432

Asp Lys Ser Met Val Ala Met Ser Asp Tyr His Asp Tyr His Lys Thr

130 135 140

gtc aaa aga cac att ttg act gct gtc tta ggt cca aac gcc caa aag 480

Val Lys Arg His Ile Leu Thr Ala Val Leu Gly Pro Asn Ala Gln Lys

145 150 155 160

aag ttc cgt gct cac aga gac acc atg atg gaa aac gtt tcc aat gaa 528

Lys Phe Arg Ala His Arg Asp Thr Met Met Glu Asn Val Ser Asn Glu

165 170 175

ttg cat gcc ttc ttt gaa aag aac cca aac caa gaa gtc aac ttg aga 576

Leu His Ala Phe Phe Glu Lys Asn Pro Asn Gln Glu Val Asn Leu Arg

180 185 190

aag atc ttc caa tct caa ttg ttc ggt ttg gcc atg aag caa gct ttg 624

Lys Ile Phe Gln Ser Gln Leu Phe Gly Leu Ala Met Lys Gln Ala Leu

195 200 205

ggt aag gat gtc gaa tct atc tac gtc aag gac ttg gaa act acc atg 672

Gly Lys Asp Val Glu Ser Ile Tyr Val Lys Asp Leu Glu Thr Thr Met

210 215 220

aag aga gaa gaa atc ttt gaa gtc ttg gtt gtt gac cca atg atg ggt 720

Lys Arg Glu Glu Ile Phe Glu Val Leu Val Val Asp Pro Met Met Gly

225 230 235 240

gcc att gaa gtc gat tgg aga gac ttc ttc cca tac ttg aaa tgg gtt 768

Ala Ile Glu Val Asp Trp Arg Asp Phe Phe Pro Tyr Leu Lys Trp Val

245 250 255

cca aac aaa tct ttc gaa aac atc att cac aga atg tac acc cgt cgt 816

Pro Asn Lys Ser Phe Glu Asn Ile Ile His Arg Met Tyr Thr Arg Arg

260 265 270

gaa gct gtc atg aag gct ttg atc caa gaa cac aag aag aga att gct 864

Glu Ala Val Met Lys Ala Leu Ile Gln Glu His Lys Lys Arg Ile Ala

275 280 285

tct ggt gaa aac tta aac tcc tac att gac tac ttg ttg tct gaa gct 912

Ser Gly Glu Asn Leu Asn Ser Tyr Ile Asp Tyr Leu Leu Ser Glu Ala

290 295 300

caa act ttg act gac aag caa ttg ttg atg tcc cta tgg gaa cca atc 960

Gln Thr Leu Thr Asp Lys Gln Leu Leu Met Ser Leu Trp Glu Pro Ile

305 310 315 320

att gaa tct tcc gac acc acc atg gtc acc act gaa tgg gct atg tac 1008

Ile Glu Ser Ser Asp Thr Thr Met Val Thr Thr Glu Trp Ala Met Tyr

325 330 335

gaa ttg gct aag aat cca aac atg caa gac aga ttg tac gaa gaa atc 1056

Glu Leu Ala Lys Asn Pro Asn Met Gln Asp Arg Leu Tyr Glu Glu Ile

340 345 350

caa tct gtt tgt ggt tcc gaa aag atc act gaa gaa aac ttg tct caa 1104

Gln Ser Val Cys Gly Ser Glu Lys Ile Thr Glu Glu Asn Leu Ser Gln

355 360 365

tta cca tac ttg tac gct gtt ttc caa gaa act ttg aga aag cac tgt 1152

Leu Pro Tyr Leu Tyr Ala Val Phe Gln Glu Thr Leu Arg Lys His Cys

370 375 380

cca gtt cca atc atg cca ttg aga tac gtc cac gaa aac acc gtt ttg 1200

Pro Val Pro Ile Met Pro Leu Arg Tyr Val His Glu Asn Thr Val Leu

385 390 395 400

ggt ggt tac cac gtt cca gct ggt act gaa gtt gct atc aac atc tat 1248

Gly Gly Tyr His Val Pro Ala Gly Thr Glu Val Ala Ile Asn Ile Tyr

405 410 415

ggt tgt aac atg gac aag aag gtc tgg gaa aac cca gaa gaa tgg aac 1296

Gly Cys Asn Met Asp Lys Lys Val Trp Glu Asn Pro Glu Glu Trp Asn

420 425 430

cca gaa aga ttc tta tcc gaa aag gaa tcc atg gac ttg tac aag acc 1344

Pro Glu Arg Phe Leu Ser Glu Lys Glu Ser Met Asp Leu Tyr Lys Thr

435 440 445

atg gcc ttc ggt ggt ggt aag aga gtt tgt gct ggt tct ttg caa gct 1392

Met Ala Phe Gly Gly Gly Lys Arg Val Cys Ala Gly Ser Leu Gln Ala

450 455 460

atg gtc atc tct tgt atc ggt att ggt aga tta gtc caa gat ttt gaa 1440

Met Val Ile Ser Cys Ile Gly Ile Gly Arg Leu Val Gln Asp Phe Glu

465 470 475 480

tgg aaa ttg aaa gat gac gct gaa gaa gat gtc aac act tta ggt tta 1488

Trp Lys Leu Lys Asp Asp Ala Glu Glu Asp Val Asn Thr Leu Gly Leu

485 490 495

acc act caa aag ttg cac cca tta ttg gct ttg atc aac cct cga aag 1536

Thr Thr Gln Lys Leu His Pro Leu Leu Ala Leu Ile Asn Pro Arg Lys

500 505 510

<210> 24

<211> 512

<212> PRT

<213> Lactuca sativa

<400> 24

Met Asp Gly Val Ile Asp Met Gln Thr Ile Pro Leu Arg Thr Ala Ile

1 5 10 15

Ala Ile Gly Gly Thr Ala Val Ala Leu Val Val Ala Leu Tyr Phe Trp

20 25 30

Phe Leu Arg Ser Tyr Ala Ser Pro Ser His His Ser Asn His Leu Pro

35 40 45

Pro Val Pro Glu Val Pro Gly Val Pro Val Leu Gly Asn Leu Leu Gln

50 55 60

Leu Lys Glu Lys Lys Pro Tyr Met Thr Phe Thr Lys Trp Ala Glu Met

65 70 75 80

Tyr Gly Pro Ile Tyr Ser Ile Arg Thr Gly Ala Thr Ser Met Val Val

85 90 95

Val Ser Ser Asn Glu Ile Ala Lys Glu Val Val Val Thr Arg Phe Pro

100 105 110

Ser Ile Ser Thr Arg Lys Leu Ser Tyr Ala Leu Lys Val Leu Thr Glu

115 120 125

Asp Lys Ser Met Val Ala Met Ser Asp Tyr His Asp Tyr His Lys Thr

130 135 140

Val Lys Arg His Ile Leu Thr Ala Val Leu Gly Pro Asn Ala Gln Lys

145 150 155 160

Lys Phe Arg Ala His Arg Asp Thr Met Met Glu Asn Val Ser Asn Glu

165 170 175

Leu His Ala Phe Phe Glu Lys Asn Pro Asn Gln Glu Val Asn Leu Arg

180 185 190

Lys Ile Phe Gln Ser Gln Leu Phe Gly Leu Ala Met Lys Gln Ala Leu

195 200 205

Gly Lys Asp Val Glu Ser Ile Tyr Val Lys Asp Leu Glu Thr Thr Met

210 215 220

Lys Arg Glu Glu Ile Phe Glu Val Leu Val Val Asp Pro Met Met Gly

225 230 235 240

Ala Ile Glu Val Asp Trp Arg Asp Phe Phe Pro Tyr Leu Lys Trp Val

245 250 255

Pro Asn Lys Ser Phe Glu Asn Ile Ile His Arg Met Tyr Thr Arg Arg

260 265 270

Glu Ala Val Met Lys Ala Leu Ile Gln Glu His Lys Lys Arg Ile Ala

275 280 285

Ser Gly Glu Asn Leu Asn Ser Tyr Ile Asp Tyr Leu Leu Ser Glu Ala

290 295 300

Gln Thr Leu Thr Asp Lys Gln Leu Leu Met Ser Leu Trp Glu Pro Ile

305 310 315 320

Ile Glu Ser Ser Asp Thr Thr Met Val Thr Thr Glu Trp Ala Met Tyr

325 330 335

Glu Leu Ala Lys Asn Pro Asn Met Gln Asp Arg Leu Tyr Glu Glu Ile

340 345 350

Gln Ser Val Cys Gly Ser Glu Lys Ile Thr Glu Glu Asn Leu Ser Gln

355 360 365

Leu Pro Tyr Leu Tyr Ala Val Phe Gln Glu Thr Leu Arg Lys His Cys

370 375 380

Pro Val Pro Ile Met Pro Leu Arg Tyr Val His Glu Asn Thr Val Leu

385 390 395 400

Gly Gly Tyr His Val Pro Ala Gly Thr Glu Val Ala Ile Asn Ile Tyr

405 410 415

Gly Cys Asn Met Asp Lys Lys Val Trp Glu Asn Pro Glu Glu Trp Asn

420 425 430

Pro Glu Arg Phe Leu Ser Glu Lys Glu Ser Met Asp Leu Tyr Lys Thr

435 440 445

Met Ala Phe Gly Gly Gly Lys Arg Val Cys Ala Gly Ser Leu Gln Ala

450 455 460

Met Val Ile Ser Cys Ile Gly Ile Gly Arg Leu Val Gln Asp Phe Glu

465 470 475 480

Trp Lys Leu Lys Asp Asp Ala Glu Glu Asp Val Asn Thr Leu Gly Leu

485 490 495

Thr Thr Gln Lys Leu His Pro Leu Leu Ala Leu Ile Asn Pro Arg Lys

500 505 510

<210> 25

<211> 1575

<212> DNA

<213> Sphaceloma manihoticola

<220>

<221> CDS

<222> (1)..(1575)

<400> 25

atg atg gac gac acc act tct cca tac tcc act tac cac tct gtc aga 48

Met Met Asp Asp Thr Thr Ser Pro Tyr Ser Thr Tyr His Ser Val Arg

1 5 10 15

tcc att aga aac caa tct gct tgg gct ttg gct cca att gct gtc ttt 96

Ser Ile Arg Asn Gln Ser Ala Trp Ala Leu Ala Pro Ile Ala Val Phe

20 25 30

att tgt tac gtt gtc ttg aga cac aac aga aag tct gtc cca gct gcc 144

Ile Cys Tyr Val Val Leu Arg His Asn Arg Lys Ser Val Pro Ala Ala

35 40 45

tcc gct ggt tct cac tcc atc ttg gaa cca tta tgg tta gct aga tta 192

Ser Ala Gly Ser His Ser Ile Leu Glu Pro Leu Trp Leu Ala Arg Leu

50 55 60

cgt ttc atc aga gac tcc aga ttc atc atc ggt caa ggt tac tcc aag 240

Arg Phe Ile Arg Asp Ser Arg Phe Ile Ile Gly Gln Gly Tyr Ser Lys

65 70 75 80

ttc aag gat act atc ttc aag gtt acc aag gtc ggt gct gac atc atc 288

Phe Lys Asp Thr Ile Phe Lys Val Thr Lys Val Gly Ala Asp Ile Ile

85 90 95

gtt gtt gct cca aag tac gtc gaa gaa atc aga aga ttg tct cgt gac 336

Val Val Ala Pro Lys Tyr Val Glu Glu Ile Arg Arg Leu Ser Arg Asp

100 105 110

acc ggt aga tct gtt gaa cca ttc atc cac gat ttc gct ggt gaa ttg 384

Thr Gly Arg Ser Val Glu Pro Phe Ile His Asp Phe Ala Gly Glu Leu

115 120 125

ttg ggt ggt ttg aat ttc ttg gaa tct gat ttg caa acc aga gtt gtc 432

Leu Gly Gly Leu Asn Phe Leu Glu Ser Asp Leu Gln Thr Arg Val Val

130 135 140

caa caa aag ttg acc cca aac ttg aaa act att gtc cca gtc atg gaa 480

Gln Gln Lys Leu Thr Pro Asn Leu Lys Thr Ile Val Pro Val Met Glu

145 150 155 160

gat gaa atg cat tac gct ttg gtt tct gaa ttg gat tct tgt ttg gac 528

Asp Glu Met His Tyr Ala Leu Val Ser Glu Leu Asp Ser Cys Leu Asp

165 170 175

ggt tcc gaa cac tgg acc aga gtt gac atg att cac atg ttg tcc aga 576

Gly Ser Glu His Trp Thr Arg Val Asp Met Ile His Met Leu Ser Arg

180 185 190

atc gtt tcc aga att tct gcc aga atc ttc ttg ggt cca aag tac tgt 624

Ile Val Ser Arg Ile Ser Ala Arg Ile Phe Leu Gly Pro Lys Tyr Cys

195 200 205

aga aac gac tta tgg tta aag acc acc gct gaa tac act gaa aac ttg 672

Arg Asn Asp Leu Trp Leu Lys Thr Thr Ala Glu Tyr Thr Glu Asn Leu

210 215 220

ttc ttg acc ggt act cta ttg aga ttt gtc cca aga atg ttg caa aag 720

Phe Leu Thr Gly Thr Leu Leu Arg Phe Val Pro Arg Met Leu Gln Lys

225 230 235 240

tgg atc gct cca tta cta cca tct ttc cgt caa ttg caa gaa aac aga 768

Trp Ile Ala Pro Leu Leu Pro Ser Phe Arg Gln Leu Gln Glu Asn Arg

245 250 255

caa gct gcc aga aag atc att tct gaa att ttg act gac cat caa cca 816

Gln Ala Ala Arg Lys Ile Ile Ser Glu Ile Leu Thr Asp His Gln Pro

260 265 270

gaa aag cac gat gaa acc tct gac aac ggt gac cca tac cca gat atc 864

Glu Lys His Asp Glu Thr Ser Asp Asn Gly Asp Pro Tyr Pro Asp Ile

275 280 285

ttg act ttg atg ttc caa gct gcc cgt ggt aag gaa aag gac atc gaa 912

Leu Thr Leu Met Phe Gln Ala Ala Arg Gly Lys Glu Lys Asp Ile Glu

290 295 300

gat atc gct caa cac act ttg ttg cta tct ttg tcc tcc att cac act 960

Asp Ile Ala Gln His Thr Leu Leu Leu Ser Leu Ser Ser Ile His Thr

305 310 315 320

acc gct ttg acc atg act caa gct ttg tac gac ttg tgt gcc tac cca 1008

Thr Ala Leu Thr Met Thr Gln Ala Leu Tyr Asp Leu Cys Ala Tyr Pro

325 330 335

caa tac ttg gac cct gtt aag cac gaa att gct gac acc ttg caa tct 1056

Gln Tyr Leu Asp Pro Val Lys His Glu Ile Ala Asp Thr Leu Gln Ser

340 345 350

gaa ggt tcc tgg tct aag gct atg ttg gac aaa ttg cac atg atg gac 1104

Glu Gly Ser Trp Ser Lys Ala Met Leu Asp Lys Leu His Met Met Asp

355 360 365

tct ttg ttg aga gaa tct caa aga tta tct cct gtt ttc tta ttg act 1152

Ser Leu Leu Arg Glu Ser Gln Arg Leu Ser Pro Val Phe Leu Leu Thr

370 375 380

ttc aac aga att ttg cac acc cca ttg act ttg tcc aac ggt atc cat 1200

Phe Asn Arg Ile Leu His Thr Pro Leu Thr Leu Ser Asn Gly Ile His

385 390 395 400

ttg cca aag ggt act aga att gct gct cct tct gat gcc att ttg aac 1248

Leu Pro Lys Gly Thr Arg Ile Ala Ala Pro Ser Asp Ala Ile Leu Asn

405 410 415

gat cca tct tta gtc cca ggt cca caa cca gct gac act ttc gac cca 1296

Asp Pro Ser Leu Val Pro Gly Pro Gln Pro Ala Asp Thr Phe Asp Pro

420 425 430

ttt aga tac atc aac cac tcc act ggt gat gct aag aaa acc aag acc 1344

Phe Arg Tyr Ile Asn His Ser Thr Gly Asp Ala Lys Lys Thr Lys Thr

435 440 445

aac ttc caa acc act tct ttg caa aac atg gct ttc ggt tac ggt aaa 1392

Asn Phe Gln Thr Thr Ser Leu Gln Asn Met Ala Phe Gly Tyr Gly Lys

450 455 460

tac gct tgt cca ggt cgt ttc tat gtt gcc aac gaa atc aaa ttg gtt 1440

Tyr Ala Cys Pro Gly Arg Phe Tyr Val Ala Asn Glu Ile Lys Leu Val

465 470 475 480

tta ggt cac ttg ttg atg cac tac gaa ttc aag ttc cca cca ggt atg 1488

Leu Gly His Leu Leu Met His Tyr Glu Phe Lys Phe Pro Pro Gly Met

485 490 495

ggt aga cca gtt aac tcc acc gtc gat act gac atg tac cca gat ttg 1536

Gly Arg Pro Val Asn Ser Thr Val Asp Thr Asp Met Tyr Pro Asp Leu

500 505 510

ggt gcc aga ttg ttg gtt aga aag aga aag atg gaa gaa 1575

Gly Ala Arg Leu Leu Val Arg Lys Arg Lys Met Glu Glu

515 520 525

<210> 26

<211> 525

<212> PRT

<213> Sphaceloma manihoticola

<400> 26

Met Met Asp Asp Thr Thr Ser Pro Tyr Ser Thr Tyr His Ser Val Arg

1 5 10 15

Ser Ile Arg Asn Gln Ser Ala Trp Ala Leu Ala Pro Ile Ala Val Phe

20 25 30

Ile Cys Tyr Val Val Leu Arg His Asn Arg Lys Ser Val Pro Ala Ala

35 40 45

Ser Ala Gly Ser His Ser Ile Leu Glu Pro Leu Trp Leu Ala Arg Leu

50 55 60

Arg Phe Ile Arg Asp Ser Arg Phe Ile Ile Gly Gln Gly Tyr Ser Lys

65 70 75 80

Phe Lys Asp Thr Ile Phe Lys Val Thr Lys Val Gly Ala Asp Ile Ile

85 90 95

Val Val Ala Pro Lys Tyr Val Glu Glu Ile Arg Arg Leu Ser Arg Asp

100 105 110

Thr Gly Arg Ser Val Glu Pro Phe Ile His Asp Phe Ala Gly Glu Leu

115 120 125

Leu Gly Gly Leu Asn Phe Leu Glu Ser Asp Leu Gln Thr Arg Val Val

130 135 140

Gln Gln Lys Leu Thr Pro Asn Leu Lys Thr Ile Val Pro Val Met Glu

145 150 155 160

Asp Glu Met His Tyr Ala Leu Val Ser Glu Leu Asp Ser Cys Leu Asp

165 170 175

Gly Ser Glu His Trp Thr Arg Val Asp Met Ile His Met Leu Ser Arg

180 185 190

Ile Val Ser Arg Ile Ser Ala Arg Ile Phe Leu Gly Pro Lys Tyr Cys

195 200 205

Arg Asn Asp Leu Trp Leu Lys Thr Thr Ala Glu Tyr Thr Glu Asn Leu

210 215 220

Phe Leu Thr Gly Thr Leu Leu Arg Phe Val Pro Arg Met Leu Gln Lys

225 230 235 240

Trp Ile Ala Pro Leu Leu Pro Ser Phe Arg Gln Leu Gln Glu Asn Arg

245 250 255

Gln Ala Ala Arg Lys Ile Ile Ser Glu Ile Leu Thr Asp His Gln Pro

260 265 270

Glu Lys His Asp Glu Thr Ser Asp Asn Gly Asp Pro Tyr Pro Asp Ile

275 280 285

Leu Thr Leu Met Phe Gln Ala Ala Arg Gly Lys Glu Lys Asp Ile Glu

290 295 300

Asp Ile Ala Gln His Thr Leu Leu Leu Ser Leu Ser Ser Ile His Thr

305 310 315 320

Thr Ala Leu Thr Met Thr Gln Ala Leu Tyr Asp Leu Cys Ala Tyr Pro

325 330 335

Gln Tyr Leu Asp Pro Val Lys His Glu Ile Ala Asp Thr Leu Gln Ser

340 345 350

Glu Gly Ser Trp Ser Lys Ala Met Leu Asp Lys Leu His Met Met Asp

355 360 365

Ser Leu Leu Arg Glu Ser Gln Arg Leu Ser Pro Val Phe Leu Leu Thr

370 375 380

Phe Asn Arg Ile Leu His Thr Pro Leu Thr Leu Ser Asn Gly Ile His

385 390 395 400

Leu Pro Lys Gly Thr Arg Ile Ala Ala Pro Ser Asp Ala Ile Leu Asn

405 410 415

Asp Pro Ser Leu Val Pro Gly Pro Gln Pro Ala Asp Thr Phe Asp Pro

420 425 430

Phe Arg Tyr Ile Asn His Ser Thr Gly Asp Ala Lys Lys Thr Lys Thr

435 440 445

Asn Phe Gln Thr Thr Ser Leu Gln Asn Met Ala Phe Gly Tyr Gly Lys

450 455 460

Tyr Ala Cys Pro Gly Arg Phe Tyr Val Ala Asn Glu Ile Lys Leu Val

465 470 475 480

Leu Gly His Leu Leu Met His Tyr Glu Phe Lys Phe Pro Pro Gly Met

485 490 495

Gly Arg Pro Val Asn Ser Thr Val Asp Thr Asp Met Tyr Pro Asp Leu

500 505 510

Gly Ala Arg Leu Leu Val Arg Lys Arg Lys Met Glu Glu

515 520 525

<210> 27

<211> 1440

<212> DNA

<213> Artemisia annua

<220>

<221> CDS

<222> (1)..(1440)

<400> 27

atg cca atg acc gtc atg ttg ttg ttc gtt ttc tta tta ttc att gcc 48

Met Pro Met Thr Val Met Leu Leu Phe Val Phe Leu Leu Phe Ile Ala

1 5 10 15

atc tgt ttc ttc ttg gtc cac aga cac aac tcc acc acc acc aag aac 96

Ile Cys Phe Phe Leu Val His Arg His Asn Ser Thr Thr Thr Lys Asn

20 25 30

ttg cca cca ggt tct ttc ggt tgg cca ttc atc ggt gaa act ttg gct 144

Leu Pro Pro Gly Ser Phe Gly Trp Pro Phe Ile Gly Glu Thr Leu Ala

35 40 45

tac atc aga tcc aag aga ggt ggt gac cca gaa aga ttc acc aag gaa 192

Tyr Ile Arg Ser Lys Arg Gly Gly Asp Pro Glu Arg Phe Thr Lys Glu

50 55 60

aga att gaa aag tac ggt tcc act tta gtc ttt aag acc tct gtt gct 240

Arg Ile Glu Lys Tyr Gly Ser Thr Leu Val Phe Lys Thr Ser Val Ala

65 70 75 80

ggt gaa aga atg gct gtc ttt tgt ggt cca gaa ggt aac aag ttc ttg 288

Gly Glu Arg Met Ala Val Phe Cys Gly Pro Glu Gly Asn Lys Phe Leu

85 90 95

ttt ggt aac gaa aac aaa ttg gtt gct tcc tgg tgg cca aac tct gtt 336

Phe Gly Asn Glu Asn Lys Leu Val Ala Ser Trp Trp Pro Asn Ser Val

100 105 110

aga att ttg ttc gaa aag tgt ttg att acc atc aga ggt gac gaa gct 384

Arg Ile Leu Phe Glu Lys Cys Leu Ile Thr Ile Arg Gly Asp Glu Ala

115 120 125

aaa tgg tta cgt aaa atg atg ttc gct tac ttg ggt cca gac gct cta 432

Lys Trp Leu Arg Lys Met Met Phe Ala Tyr Leu Gly Pro Asp Ala Leu

130 135 140

tcc aac aga tac act ggt act atg gaa gtt gtt acc aga ttg cac atc 480

Ser Asn Arg Tyr Thr Gly Thr Met Glu Val Val Thr Arg Leu His Ile

145 150 155 160

caa aac cac tgg caa ggt aag tct gaa ttg aag gtc ttt gaa acc gtt 528

Gln Asn His Trp Gln Gly Lys Ser Glu Leu Lys Val Phe Glu Thr Val

165 170 175

aga cca tac ttg ttc gaa ttg gcc tgt aga tta ttc ttg tct ttg gat 576

Arg Pro Tyr Leu Phe Glu Leu Ala Cys Arg Leu Phe Leu Ser Leu Asp

180 185 190

gac cca aag cac gtt gct gaa ttg ggt act tta ttc aac act ttc ttg 624

Asp Pro Lys His Val Ala Glu Leu Gly Thr Leu Phe Asn Thr Phe Leu

195 200 205

aag ggt ttg act gaa ttg cca atc aac att cca ggt act aga ttc tac 672

Lys Gly Leu Thr Glu Leu Pro Ile Asn Ile Pro Gly Thr Arg Phe Tyr

210 215 220

aga gcc aag aga gct gcc aac gcc atc aag aaa caa ttg att gtc atc 720

Arg Ala Lys Arg Ala Ala Asn Ala Ile Lys Lys Gln Leu Ile Val Ile

225 230 235 240

atc aag caa cgt cgt caa gct ttg aag caa gaa gat caa tct tct tct 768

Ile Lys Gln Arg Arg Gln Ala Leu Lys Gln Glu Asp Gln Ser Ser Ser

245 250 255

ttc gaa gat ttg cta tct cat ttg tta gtc tcc tct gat gaa aac ggt 816

Phe Glu Asp Leu Leu Ser His Leu Leu Val Ser Ser Asp Glu Asn Gly

260 265 270

aga ttc ttg tcc gaa gct gaa att gcc aat aat gtc ttg ttg ttg ttg 864

Arg Phe Leu Ser Glu Ala Glu Ile Ala Asn Asn Val Leu Leu Leu Leu

275 280 285

ttt gct ggt cac gac act tct gct gtt tcc atc act ttg ttg atg aag 912

Phe Ala Gly His Asp Thr Ser Ala Val Ser Ile Thr Leu Leu Met Lys

290 295 300

tct ttg gct gaa cac cct caa gtc tac gac aac gtt ttg aag gaa caa 960

Ser Leu Ala Glu His Pro Gln Val Tyr Asp Asn Val Leu Lys Glu Gln

305 310 315 320

tta ggt atc ttg gaa gcc aag gct cca ggt gaa atg ttg aac tgg gaa 1008

Leu Gly Ile Leu Glu Ala Lys Ala Pro Gly Glu Met Leu Asn Trp Glu

325 330 335

gat atc caa aag atg aga tac tcc tgg tac gtt gtt tgt gaa gtc atg 1056

Asp Ile Gln Lys Met Arg Tyr Ser Trp Tyr Val Val Cys Glu Val Met

340 345 350

aga ttg att cca cct gtt gtt ggt tct ttc aga gaa gct ttg gtt gat 1104

Arg Leu Ile Pro Pro Val Val Gly Ser Phe Arg Glu Ala Leu Val Asp

355 360 365

ttc gaa tac gct ggt tac act att cca aag ggt tgg aag atc atc tgg 1152

Phe Glu Tyr Ala Gly Tyr Thr Ile Pro Lys Gly Trp Lys Ile Ile Trp

370 375 380

tct gct gtc atg act cac aag gaa gaa aac aac ttc cca aac gct act 1200

Ser Ala Val Met Thr His Lys Glu Glu Asn Asn Phe Pro Asn Ala Thr

385 390 395 400

aag ttc gac cca tcc aga ttc gaa ggt gct ggt cca acc cca ttc acc 1248

Lys Phe Asp Pro Ser Arg Phe Glu Gly Ala Gly Pro Thr Pro Phe Thr

405 410 415

tac gtt cca ttc ggt ggt ggt cca aga atg tgt ttg ggt aag gaa ttg 1296

Tyr Val Pro Phe Gly Gly Gly Pro Arg Met Cys Leu Gly Lys Glu Leu

420 425 430

gct cgt gtc aga att ttg gtt ttc tta cat aac atc atg acc aaa ttc 1344

Ala Arg Val Arg Ile Leu Val Phe Leu His Asn Ile Met Thr Lys Phe

435 440 445

aaa tgg gac ttg ttg att cca gac gaa aag atc ggt tac gac cca ttg 1392

Lys Trp Asp Leu Leu Ile Pro Asp Glu Lys Ile Gly Tyr Asp Pro Leu

450 455 460

gct acc cca gtc aag ggt ttg cca gtc aga ttg cac cct cac caa gtg 1440

Ala Thr Pro Val Lys Gly Leu Pro Val Arg Leu His Pro His Gln Val

465 470 475 480

<210> 28

<211> 480

<212> PRT

<213> Artemisia annua

<400> 28

Met Pro Met Thr Val Met Leu Leu Phe Val Phe Leu Leu Phe Ile Ala

1 5 10 15

Ile Cys Phe Phe Leu Val His Arg His Asn Ser Thr Thr Thr Lys Asn

20 25 30

Leu Pro Pro Gly Ser Phe Gly Trp Pro Phe Ile Gly Glu Thr Leu Ala

35 40 45

Tyr Ile Arg Ser Lys Arg Gly Gly Asp Pro Glu Arg Phe Thr Lys Glu

50 55 60

Arg Ile Glu Lys Tyr Gly Ser Thr Leu Val Phe Lys Thr Ser Val Ala

65 70 75 80

Gly Glu Arg Met Ala Val Phe Cys Gly Pro Glu Gly Asn Lys Phe Leu

85 90 95

Phe Gly Asn Glu Asn Lys Leu Val Ala Ser Trp Trp Pro Asn Ser Val

100 105 110

Arg Ile Leu Phe Glu Lys Cys Leu Ile Thr Ile Arg Gly Asp Glu Ala

115 120 125

Lys Trp Leu Arg Lys Met Met Phe Ala Tyr Leu Gly Pro Asp Ala Leu

130 135 140

Ser Asn Arg Tyr Thr Gly Thr Met Glu Val Val Thr Arg Leu His Ile

145 150 155 160

Gln Asn His Trp Gln Gly Lys Ser Glu Leu Lys Val Phe Glu Thr Val

165 170 175

Arg Pro Tyr Leu Phe Glu Leu Ala Cys Arg Leu Phe Leu Ser Leu Asp

180 185 190

Asp Pro Lys His Val Ala Glu Leu Gly Thr Leu Phe Asn Thr Phe Leu

195 200 205

Lys Gly Leu Thr Glu Leu Pro Ile Asn Ile Pro Gly Thr Arg Phe Tyr

210 215 220

Arg Ala Lys Arg Ala Ala Asn Ala Ile Lys Lys Gln Leu Ile Val Ile

225 230 235 240

Ile Lys Gln Arg Arg Gln Ala Leu Lys Gln Glu Asp Gln Ser Ser Ser

245 250 255

Phe Glu Asp Leu Leu Ser His Leu Leu Val Ser Ser Asp Glu Asn Gly

260 265 270

Arg Phe Leu Ser Glu Ala Glu Ile Ala Asn Asn Val Leu Leu Leu Leu

275 280 285

Phe Ala Gly His Asp Thr Ser Ala Val Ser Ile Thr Leu Leu Met Lys

290 295 300

Ser Leu Ala Glu His Pro Gln Val Tyr Asp Asn Val Leu Lys Glu Gln

305 310 315 320

Leu Gly Ile Leu Glu Ala Lys Ala Pro Gly Glu Met Leu Asn Trp Glu

325 330 335

Asp Ile Gln Lys Met Arg Tyr Ser Trp Tyr Val Val Cys Glu Val Met

340 345 350

Arg Leu Ile Pro Pro Val Val Gly Ser Phe Arg Glu Ala Leu Val Asp

355 360 365

Phe Glu Tyr Ala Gly Tyr Thr Ile Pro Lys Gly Trp Lys Ile Ile Trp

370 375 380

Ser Ala Val Met Thr His Lys Glu Glu Asn Asn Phe Pro Asn Ala Thr

385 390 395 400

Lys Phe Asp Pro Ser Arg Phe Glu Gly Ala Gly Pro Thr Pro Phe Thr

405 410 415

Tyr Val Pro Phe Gly Gly Gly Pro Arg Met Cys Leu Gly Lys Glu Leu

420 425 430

Ala Arg Val Arg Ile Leu Val Phe Leu His Asn Ile Met Thr Lys Phe

435 440 445

Lys Trp Asp Leu Leu Ile Pro Asp Glu Lys Ile Gly Tyr Asp Pro Leu

450 455 460

Ala Thr Pro Val Lys Gly Leu Pro Val Arg Leu His Pro His Gln Val

465 470 475 480

<210> 29

<211> 1365

<212> DNA

<213> Ricinus communis

<220>

<221> CDS

<222> (1)..(1365)

<400> 29

atg gaa ttg gtc atg ttc cca gtc ttg gct ttg gtt tcc act ttg ttc 48

Met Glu Leu Val Met Phe Pro Val Leu Ala Leu Val Ser Thr Leu Phe

1 5 10 15

ttg ttg gct ttg cac ttc atc atc aga act ttg aag gaa aga tta ttc 96

Leu Leu Ala Leu His Phe Ile Ile Arg Thr Leu Lys Glu Arg Leu Phe

20 25 30

ggt tct cca aat ttg cca cca ggt aga tta ggt tgg cca ttg att ggt 144

Gly Ser Pro Asn Leu Pro Pro Gly Arg Leu Gly Trp Pro Leu Ile Gly

35 40 45

gaa acc cca gct ttc ttc aga gct ggt ttc gaa gcc aag cca gaa aag 192

Glu Thr Pro Ala Phe Phe Arg Ala Gly Phe Glu Ala Lys Pro Glu Lys

50 55 60

ttc atc ggt gaa aga atg gaa aag tac gac tct cgt gtt ttc aag acc 240

Phe Ile Gly Glu Arg Met Glu Lys Tyr Asp Ser Arg Val Phe Lys Thr

65 70 75 80

tct ttg ttg ggt aag cct ttc gct gtc att tct ggt act gct ggt cac 288

Ser Leu Leu Gly Lys Pro Phe Ala Val Ile Ser Gly Thr Ala Gly His

85 90 95

aag ttc ttg ttt tcc aac gaa aac aaa ttg gtt aac ttg tgg tgg cca 336

Lys Phe Leu Phe Ser Asn Glu Asn Lys Leu Val Asn Leu Trp Trp Pro

100 105 110

gaa tcc gtc aga atg ttg ttc aag tct gct ttg gtt tcc gtt gtt ggt 384

Glu Ser Val Arg Met Leu Phe Lys Ser Ala Leu Val Ser Val Val Gly

115 120 125

gac gaa gcc aag aga atc aga aag atg ttg atg act ttc tta ggt ttg 432

Asp Glu Ala Lys Arg Ile Arg Lys Met Leu Met Thr Phe Leu Gly Leu

130 135 140

gat gct ttg aag aac tac act gaa aga att gac atg gtc acc caa caa 480

Asp Ala Leu Lys Asn Tyr Thr Glu Arg Ile Asp Met Val Thr Gln Gln

145 150 155 160

cac atc aga acc tac tgg gaa ggt aag gaa gaa gtt acc gtt tac tcc 528

His Ile Arg Thr Tyr Trp Glu Gly Lys Glu Glu Val Thr Val Tyr Ser

165 170 175

act ttg aaa ttg tac act ttc acc ttg gct tgt aac ttg ttt gcc tcc 576

Thr Leu Lys Leu Tyr Thr Phe Thr Leu Ala Cys Asn Leu Phe Ala Ser

180 185 190

atc aac gac cct gaa aga tta tcc aag ttg ggt gct cac ttc gat gtt 624

Ile Asn Asp Pro Glu Arg Leu Ser Lys Leu Gly Ala His Phe Asp Val

195 200 205

ttc gtt aag ggt gtt atc tct ttg cca att tcc att cca ggt act aga 672

Phe Val Lys Gly Val Ile Ser Leu Pro Ile Ser Ile Pro Gly Thr Arg

210 215 220

tta tac aaa tcc atg aag gct gcc aac gcc att aga gaa gaa ttg aaa 720

Leu Tyr Lys Ser Met Lys Ala Ala Asn Ala Ile Arg Glu Glu Leu Lys

225 230 235 240

ttg att gtc cgt gac aga aag gaa gct ttg gaa aga aag atg gct tct 768

Leu Ile Val Arg Asp Arg Lys Glu Ala Leu Glu Arg Lys Met Ala Ser

245 250 255

cca act caa gat ttg ttg tct tat ttg ttg gtc gac tct gac acc aac 816

Pro Thr Gln Asp Leu Leu Ser Tyr Leu Leu Val Asp Ser Asp Thr Asn

260 265 270

ggt cgt ttc ttg tct gaa atg gaa atc ttg gac aac atc atg ttg cta 864

Gly Arg Phe Leu Ser Glu Met Glu Ile Leu Asp Asn Ile Met Leu Leu

275 280 285

ttg tac gct gaa caa ttg gaa att gct aac tcc aag aag cca ggt gaa 912

Leu Tyr Ala Glu Gln Leu Glu Ile Ala Asn Ser Lys Lys Pro Gly Glu

290 295 300

tta ttg caa tgg gaa gat gtc caa aag atg aga tac tcc tgg aac gtc 960

Leu Leu Gln Trp Glu Asp Val Gln Lys Met Arg Tyr Ser Trp Asn Val

305 310 315 320

atc tct gaa gtc ttg aga ttg tct cca cca gtt tct tct gct tac aga 1008

Ile Ser Glu Val Leu Arg Leu Ser Pro Pro Val Ser Ser Ala Tyr Arg

325 330 335

cac gcc att gtt gac ttt acc tac gaa ggt tac acc att cca aag ggt 1056

His Ala Ile Val Asp Phe Thr Tyr Glu Gly Tyr Thr Ile Pro Lys Gly

340 345 350

tgg caa ttg ttc acc tct ttc ggt act acc cac cgt gac cca gct tta 1104

Trp Gln Leu Phe Thr Ser Phe Gly Thr Thr His Arg Asp Pro Ala Leu

355 360 365

ttc cca aac cca gaa aga ttc gat gct tcc aga ttt gaa ggt aac ggt 1152

Phe Pro Asn Pro Glu Arg Phe Asp Ala Ser Arg Phe Glu Gly Asn Gly

370 375 380

cca cct tct tac tct tac atc cca ttc ggt ggt ggt cca aga atg tgt 1200

Pro Pro Ser Tyr Ser Tyr Ile Pro Phe Gly Gly Gly Pro Arg Met Cys

385 390 395 400

att ggt tac gaa ttt gcc aga ttg gaa atg ttg atc ttc tta cat aac 1248

Ile Gly Tyr Glu Phe Ala Arg Leu Glu Met Leu Ile Phe Leu His Asn

405 410 415

atc atc aag aga ttc aaa tgg gat atc ttg atc cca gat gaa caa ttc 1296

Ile Ile Lys Arg Phe Lys Trp Asp Ile Leu Ile Pro Asp Glu Gln Phe

420 425 430

ggt tac aac cca tta ttg gct cca tct caa ggt ttc cca gtc aga tta 1344

Gly Tyr Asn Pro Leu Leu Ala Pro Ser Gln Gly Phe Pro Val Arg Leu

435 440 445

aga cca cac cac tct cat cta 1365

Arg Pro His His Ser His Leu

450 455

<210> 30

<211> 455

<212> PRT

<213> Ricinus communis

<400> 30

Met Glu Leu Val Met Phe Pro Val Leu Ala Leu Val Ser Thr Leu Phe

1 5 10 15

Leu Leu Ala Leu His Phe Ile Ile Arg Thr Leu Lys Glu Arg Leu Phe

20 25 30

Gly Ser Pro Asn Leu Pro Pro Gly Arg Leu Gly Trp Pro Leu Ile Gly

35 40 45

Glu Thr Pro Ala Phe Phe Arg Ala Gly Phe Glu Ala Lys Pro Glu Lys

50 55 60

Phe Ile Gly Glu Arg Met Glu Lys Tyr Asp Ser Arg Val Phe Lys Thr

65 70 75 80

Ser Leu Leu Gly Lys Pro Phe Ala Val Ile Ser Gly Thr Ala Gly His

85 90 95

Lys Phe Leu Phe Ser Asn Glu Asn Lys Leu Val Asn Leu Trp Trp Pro

100 105 110

Glu Ser Val Arg Met Leu Phe Lys Ser Ala Leu Val Ser Val Val Gly

115 120 125

Asp Glu Ala Lys Arg Ile Arg Lys Met Leu Met Thr Phe Leu Gly Leu

130 135 140

Asp Ala Leu Lys Asn Tyr Thr Glu Arg Ile Asp Met Val Thr Gln Gln

145 150 155 160

His Ile Arg Thr Tyr Trp Glu Gly Lys Glu Glu Val Thr Val Tyr Ser

165 170 175

Thr Leu Lys Leu Tyr Thr Phe Thr Leu Ala Cys Asn Leu Phe Ala Ser

180 185 190

Ile Asn Asp Pro Glu Arg Leu Ser Lys Leu Gly Ala His Phe Asp Val

195 200 205

Phe Val Lys Gly Val Ile Ser Leu Pro Ile Ser Ile Pro Gly Thr Arg

210 215 220

Leu Tyr Lys Ser Met Lys Ala Ala Asn Ala Ile Arg Glu Glu Leu Lys

225 230 235 240

Leu Ile Val Arg Asp Arg Lys Glu Ala Leu Glu Arg Lys Met Ala Ser

245 250 255

Pro Thr Gln Asp Leu Leu Ser Tyr Leu Leu Val Asp Ser Asp Thr Asn

260 265 270

Gly Arg Phe Leu Ser Glu Met Glu Ile Leu Asp Asn Ile Met Leu Leu

275 280 285

Leu Tyr Ala Glu Gln Leu Glu Ile Ala Asn Ser Lys Lys Pro Gly Glu

290 295 300

Leu Leu Gln Trp Glu Asp Val Gln Lys Met Arg Tyr Ser Trp Asn Val

305 310 315 320

Ile Ser Glu Val Leu Arg Leu Ser Pro Pro Val Ser Ser Ala Tyr Arg

325 330 335

His Ala Ile Val Asp Phe Thr Tyr Glu Gly Tyr Thr Ile Pro Lys Gly

340 345 350

Trp Gln Leu Phe Thr Ser Phe Gly Thr Thr His Arg Asp Pro Ala Leu

355 360 365

Phe Pro Asn Pro Glu Arg Phe Asp Ala Ser Arg Phe Glu Gly Asn Gly

370 375 380

Pro Pro Ser Tyr Ser Tyr Ile Pro Phe Gly Gly Gly Pro Arg Met Cys

385 390 395 400

Ile Gly Tyr Glu Phe Ala Arg Leu Glu Met Leu Ile Phe Leu His Asn

405 410 415

Ile Ile Lys Arg Phe Lys Trp Asp Ile Leu Ile Pro Asp Glu Gln Phe

420 425 430

Gly Tyr Asn Pro Leu Leu Ala Pro Ser Gln Gly Phe Pro Val Arg Leu

435 440 445

Arg Pro His His Ser His Leu

450 455

<210> 31

<211> 1428

<212> DNA

<213> Stevia rebaudiana

<220>

<221> CDS

<222> (1)..(1428)

<400> 31

atg att caa gtt ttg acc cca atc ttg ttg ttc ttg atc ttc ttc gtc 48

Met Ile Gln Val Leu Thr Pro Ile Leu Leu Phe Leu Ile Phe Phe Val

1 5 10 15

ttt tgg aag gtc tac aag cac caa aag acc aag atc aac ttg cct cca 96

Phe Trp Lys Val Tyr Lys His Gln Lys Thr Lys Ile Asn Leu Pro Pro

20 25 30

ggt tct ttc ggt tgg cca ttc ttg ggt gaa act tta gct ttg ttg aga 144

Gly Ser Phe Gly Trp Pro Phe Leu Gly Glu Thr Leu Ala Leu Leu Arg

35 40 45

gct ggt tgg gac tct gaa cca gaa aga ttc gtt aga gaa aga atc aag 192

Ala Gly Trp Asp Ser Glu Pro Glu Arg Phe Val Arg Glu Arg Ile Lys

50 55 60

aag cat ggt tct cca ttg gtt ttc aag acc tct ttg ttc ggt gac aga 240

Lys His Gly Ser Pro Leu Val Phe Lys Thr Ser Leu Phe Gly Asp Arg

65 70 75 80

ttc gct gtc ttg tgt ggt cca gct ggt aac aaa ttc ttg ttc tgt aac 288

Phe Ala Val Leu Cys Gly Pro Ala Gly Asn Lys Phe Leu Phe Cys Asn

85 90 95

gaa aac aaa ttg gtt gct tcc tgg tgg cca gtt cca gtt aga aag ttg 336

Glu Asn Lys Leu Val Ala Ser Trp Trp Pro Val Pro Val Arg Lys Leu

100 105 110

ttc ggt aag tcc ttg ttg act atc aga ggt gac gaa gcc aag tgg atg 384

Phe Gly Lys Ser Leu Leu Thr Ile Arg Gly Asp Glu Ala Lys Trp Met

115 120 125

aga aag atg tta ttg tct tac ttg ggt cca gat gcc ttt gct acc cac 432

Arg Lys Met Leu Leu Ser Tyr Leu Gly Pro Asp Ala Phe Ala Thr His

130 135 140

tac gct gtc acc atg gac gtt gtt acc cgt cgt cac att gat gtc cac 480

Tyr Ala Val Thr Met Asp Val Val Thr Arg Arg His Ile Asp Val His

145 150 155 160

tgg aga ggt aag gaa gaa gtt aac gtt ttc caa acc gtc aaa ttg tac 528

Trp Arg Gly Lys Glu Glu Val Asn Val Phe Gln Thr Val Lys Leu Tyr

165 170 175

gct ttc gaa ttg gct tgt aga tta ttc atg aac ttg gat gac cca aac 576

Ala Phe Glu Leu Ala Cys Arg Leu Phe Met Asn Leu Asp Asp Pro Asn

180 185 190

cat att gcc aaa tta ggt tct tta ttc aac atc ttc ttg aag ggt atc 624

His Ile Ala Lys Leu Gly Ser Leu Phe Asn Ile Phe Leu Lys Gly Ile

195 200 205

atc gaa ttg cca att gat gtt cca ggt act aga ttc tac tcc tcc aag 672

Ile Glu Leu Pro Ile Asp Val Pro Gly Thr Arg Phe Tyr Ser Ser Lys

210 215 220

aag gct gct gct gcc atc aga att gaa ttg aag aaa ttg atc aag gcc 720

Lys Ala Ala Ala Ala Ile Arg Ile Glu Leu Lys Lys Leu Ile Lys Ala

225 230 235 240

aga aag ttg gaa ttg aag gaa ggt aag gct tct tcc tct caa gat ttg 768

Arg Lys Leu Glu Leu Lys Glu Gly Lys Ala Ser Ser Ser Gln Asp Leu

245 250 255

cta tcc cac tta ttg acc tct cca gac gaa aat ggt atg ttc ttg act 816

Leu Ser His Leu Leu Thr Ser Pro Asp Glu Asn Gly Met Phe Leu Thr

260 265 270

gaa gaa gaa att gtc gac aac atc tta ttg cta ttg ttc gct ggt cac 864

Glu Glu Glu Ile Val Asp Asn Ile Leu Leu Leu Leu Phe Ala Gly His

275 280 285

gac acc tct gct cta tcc att act ttg ttg atg aaa act ttg ggt gaa 912

Asp Thr Ser Ala Leu Ser Ile Thr Leu Leu Met Lys Thr Leu Gly Glu

290 295 300

cac tct gat gtt tac gac aag gtc tta aag gaa caa ttg gaa atc tcc 960

His Ser Asp Val Tyr Asp Lys Val Leu Lys Glu Gln Leu Glu Ile Ser

305 310 315 320

aag act aag gaa gct tgg gaa tct ttg aaa tgg gaa gat atc caa aag 1008

Lys Thr Lys Glu Ala Trp Glu Ser Leu Lys Trp Glu Asp Ile Gln Lys

325 330 335

atg aag tac tcc tgg tct gtt atc tgt gaa gtc atg aga ttg aac cct 1056

Met Lys Tyr Ser Trp Ser Val Ile Cys Glu Val Met Arg Leu Asn Pro

340 345 350

cca gtc att ggt act tac aga gaa gct ttg gtc gat atc gac tac gct 1104

Pro Val Ile Gly Thr Tyr Arg Glu Ala Leu Val Asp Ile Asp Tyr Ala

355 360 365

ggt tac acc att cca aag ggt tgg aag ttg cac tgg tct gcc gtt tcc 1152

Gly Tyr Thr Ile Pro Lys Gly Trp Lys Leu His Trp Ser Ala Val Ser

370 375 380

act caa aga gat gaa gct aac ttc gaa gat gtc acc aga ttt gac cca 1200

Thr Gln Arg Asp Glu Ala Asn Phe Glu Asp Val Thr Arg Phe Asp Pro

385 390 395 400

tct cgt ttc gaa ggt gct ggt cca act cca ttc act ttt gtt cca ttc 1248

Ser Arg Phe Glu Gly Ala Gly Pro Thr Pro Phe Thr Phe Val Pro Phe

405 410 415

ggt ggt ggt cca aga atg tgt ttg ggt aag gaa ttc gct aga tta gaa 1296

Gly Gly Gly Pro Arg Met Cys Leu Gly Lys Glu Phe Ala Arg Leu Glu

420 425 430

gtt ttg gct ttc ttg cac aac att gtc acc aac ttt aaa tgg gac ttg 1344

Val Leu Ala Phe Leu His Asn Ile Val Thr Asn Phe Lys Trp Asp Leu

435 440 445

ttg att cca gac gaa aag atc gaa tac gac cca atg gct act cca gcc 1392

Leu Ile Pro Asp Glu Lys Ile Glu Tyr Asp Pro Met Ala Thr Pro Ala

450 455 460

aag ggt ttg cca atc aga ttg cac cct cac caa gtc 1428

Lys Gly Leu Pro Ile Arg Leu His Pro His Gln Val

465 470 475

<210> 32

<211> 476

<212> PRT

<213> Stevia rebaudiana

<400> 32

Met Ile Gln Val Leu Thr Pro Ile Leu Leu Phe Leu Ile Phe Phe Val

1 5 10 15

Phe Trp Lys Val Tyr Lys His Gln Lys Thr Lys Ile Asn Leu Pro Pro

20 25 30

Gly Ser Phe Gly Trp Pro Phe Leu Gly Glu Thr Leu Ala Leu Leu Arg

35 40 45

Ala Gly Trp Asp Ser Glu Pro Glu Arg Phe Val Arg Glu Arg Ile Lys

50 55 60

Lys His Gly Ser Pro Leu Val Phe Lys Thr Ser Leu Phe Gly Asp Arg

65 70 75 80

Phe Ala Val Leu Cys Gly Pro Ala Gly Asn Lys Phe Leu Phe Cys Asn

85 90 95

Glu Asn Lys Leu Val Ala Ser Trp Trp Pro Val Pro Val Arg Lys Leu

100 105 110

Phe Gly Lys Ser Leu Leu Thr Ile Arg Gly Asp Glu Ala Lys Trp Met

115 120 125

Arg Lys Met Leu Leu Ser Tyr Leu Gly Pro Asp Ala Phe Ala Thr His

130 135 140

Tyr Ala Val Thr Met Asp Val Val Thr Arg Arg His Ile Asp Val His

145 150 155 160

Trp Arg Gly Lys Glu Glu Val Asn Val Phe Gln Thr Val Lys Leu Tyr

165 170 175

Ala Phe Glu Leu Ala Cys Arg Leu Phe Met Asn Leu Asp Asp Pro Asn

180 185 190

His Ile Ala Lys Leu Gly Ser Leu Phe Asn Ile Phe Leu Lys Gly Ile

195 200 205

Ile Glu Leu Pro Ile Asp Val Pro Gly Thr Arg Phe Tyr Ser Ser Lys

210 215 220

Lys Ala Ala Ala Ala Ile Arg Ile Glu Leu Lys Lys Leu Ile Lys Ala

225 230 235 240

Arg Lys Leu Glu Leu Lys Glu Gly Lys Ala Ser Ser Ser Gln Asp Leu

245 250 255

Leu Ser His Leu Leu Thr Ser Pro Asp Glu Asn Gly Met Phe Leu Thr

260 265 270

Glu Glu Glu Ile Val Asp Asn Ile Leu Leu Leu Leu Phe Ala Gly His

275 280 285

Asp Thr Ser Ala Leu Ser Ile Thr Leu Leu Met Lys Thr Leu Gly Glu

290 295 300

His Ser Asp Val Tyr Asp Lys Val Leu Lys Glu Gln Leu Glu Ile Ser

305 310 315 320

Lys Thr Lys Glu Ala Trp Glu Ser Leu Lys Trp Glu Asp Ile Gln Lys

325 330 335

Met Lys Tyr Ser Trp Ser Val Ile Cys Glu Val Met Arg Leu Asn Pro

340 345 350

Pro Val Ile Gly Thr Tyr Arg Glu Ala Leu Val Asp Ile Asp Tyr Ala

355 360 365

Gly Tyr Thr Ile Pro Lys Gly Trp Lys Leu His Trp Ser Ala Val Ser

370 375 380

Thr Gln Arg Asp Glu Ala Asn Phe Glu Asp Val Thr Arg Phe Asp Pro

385 390 395 400

Ser Arg Phe Glu Gly Ala Gly Pro Thr Pro Phe Thr Phe Val Pro Phe

405 410 415

Gly Gly Gly Pro Arg Met Cys Leu Gly Lys Glu Phe Ala Arg Leu Glu

420 425 430

Val Leu Ala Phe Leu His Asn Ile Val Thr Asn Phe Lys Trp Asp Leu

435 440 445

Leu Ile Pro Asp Glu Lys Ile Glu Tyr Asp Pro Met Ala Thr Pro Ala

450 455 460

Lys Gly Leu Pro Ile Arg Leu His Pro His Gln Val

465 470 475

<210> 33

<211> 1575

<212> DNA

<213> Arabidopsis thaliana

<220>

<221> CDS

<222> (1)..(1575)

<400> 33

atg gaa tct tta gtc gtt cac acc gtc aat gcc atc tgg tgt att gtc 48

Met Glu Ser Leu Val Val His Thr Val Asn Ala Ile Trp Cys Ile Val

1 5 10 15

att gtt ggt att ttc tct gtt ggt tac cac gtt tac ggt cgt gcc gtt 96

Ile Val Gly Ile Phe Ser Val Gly Tyr His Val Tyr Gly Arg Ala Val

20 25 30

gtt gaa caa tgg aga atg aga aga tct ttg aaa ttg caa ggt gtc aag 144

Val Glu Gln Trp Arg Met Arg Arg Ser Leu Lys Leu Gln Gly Val Lys

35 40 45

ggt cca cca cca tcc att ttc aac ggt aat gtc tct gaa atg caa aga 192

Gly Pro Pro Pro Ser Ile Phe Asn Gly Asn Val Ser Glu Met Gln Arg

50 55 60

atc caa tct gaa gct aag cac tgt tcc ggt gac aac atc att tct cac 240

Ile Gln Ser Glu Ala Lys His Cys Ser Gly Asp Asn Ile Ile Ser His

65 70 75 80

gat tac tcc tcc tct ttg ttc cct cac ttt gac cac tgg aga aag caa 288

Asp Tyr Ser Ser Ser Leu Phe Pro His Phe Asp His Trp Arg Lys Gln

85 90 95

tac ggt aga atc tac acc tac tcc act ggt ttg aaa caa cat ttg tac 336

Tyr Gly Arg Ile Tyr Thr Tyr Ser Thr Gly Leu Lys Gln His Leu Tyr

100 105 110

atc aac cat cca gaa atg gtc aag gaa tta tct caa acc aac act ttg 384

Ile Asn His Pro Glu Met Val Lys Glu Leu Ser Gln Thr Asn Thr Leu

115 120 125

aac tta ggt cgt atc act cac atc acc aag aga ttg aac cca atc tta 432

Asn Leu Gly Arg Ile Thr His Ile Thr Lys Arg Leu Asn Pro Ile Leu

130 135 140

ggt aac ggt atc atc act tcc aac ggt cca cac tgg gct cat caa aga 480

Gly Asn Gly Ile Ile Thr Ser Asn Gly Pro His Trp Ala His Gln Arg

145 150 155 160

aga att att gct tac gaa ttc acc cac gac aaa atc aag ggt atg gtc 528

Arg Ile Ile Ala Tyr Glu Phe Thr His Asp Lys Ile Lys Gly Met Val

165 170 175

ggt ttg atg gtc gaa tct gcc atg cca atg ttg aac aaa tgg gaa gaa 576

Gly Leu Met Val Glu Ser Ala Met Pro Met Leu Asn Lys Trp Glu Glu

180 185 190

atg gtt aag aga ggt ggt gaa atg ggt tgt gac atc cgt gtt gac gaa 624

Met Val Lys Arg Gly Gly Glu Met Gly Cys Asp Ile Arg Val Asp Glu

195 200 205

gat ttg aag gat gtt tct gct gat gtc att gct aag gct tgt ttc ggt 672

Asp Leu Lys Asp Val Ser Ala Asp Val Ile Ala Lys Ala Cys Phe Gly

210 215 220

tcc tct ttc tcc aag ggt aag gct atc ttc tcc atg atc aga gac ttg 720

Ser Ser Phe Ser Lys Gly Lys Ala Ile Phe Ser Met Ile Arg Asp Leu

225 230 235 240

ttg act gcc atc act aag aga tct gtt ttg ttc aga ttc aac ggt ttc 768

Leu Thr Ala Ile Thr Lys Arg Ser Val Leu Phe Arg Phe Asn Gly Phe

245 250 255

acc gac atg gtt ttc ggt tcc aag aag cat ggt gat gtc gat atc gat 816

Thr Asp Met Val Phe Gly Ser Lys Lys His Gly Asp Val Asp Ile Asp

260 265 270

gct ttg gaa atg gaa ttg gaa tct tct atc tgg gaa acc gtt aag gaa 864

Ala Leu Glu Met Glu Leu Glu Ser Ser Ile Trp Glu Thr Val Lys Glu

275 280 285

aga gaa att gaa tgt aag gac act cac aag aag gat ttg atg caa tta 912

Arg Glu Ile Glu Cys Lys Asp Thr His Lys Lys Asp Leu Met Gln Leu

290 295 300

atc ttg gaa ggt gcc atg aga tct tgt gac ggt aac ttg tgg gac aag 960

Ile Leu Glu Gly Ala Met Arg Ser Cys Asp Gly Asn Leu Trp Asp Lys

305 310 315 320

tct gct tac aga aga ttt gtt gtc gac aac tgt aaa tcc atc tac ttt 1008

Ser Ala Tyr Arg Arg Phe Val Val Asp Asn Cys Lys Ser Ile Tyr Phe

325 330 335

gcc ggt cac gac tct act gct gtc tcc gtt tcc tgg tgt ttg atg ttg 1056

Ala Gly His Asp Ser Thr Ala Val Ser Val Ser Trp Cys Leu Met Leu

340 345 350

cta gct ttg aac cca tcc tgg caa gtc aag atc aga gat gaa atc tta 1104

Leu Ala Leu Asn Pro Ser Trp Gln Val Lys Ile Arg Asp Glu Ile Leu

355 360 365

tct tct tgt aag aac ggt att cca gat gct gaa tcc att cca aac ttg 1152

Ser Ser Cys Lys Asn Gly Ile Pro Asp Ala Glu Ser Ile Pro Asn Leu

370 375 380

aag acc gtt acc atg gtc att caa gaa act atg aga ttg tac cca cca 1200

Lys Thr Val Thr Met Val Ile Gln Glu Thr Met Arg Leu Tyr Pro Pro

385 390 395 400

gct cca att gtc ggt aga gaa gct tcc aag gac atc aga tta ggt gac 1248

Ala Pro Ile Val Gly Arg Glu Ala Ser Lys Asp Ile Arg Leu Gly Asp

405 410 415

ttg gtt gtt cca aag ggt gtt tgt atc tgg act ttg att cca gct ttg 1296

Leu Val Val Pro Lys Gly Val Cys Ile Trp Thr Leu Ile Pro Ala Leu

420 425 430

cac cgt gac cca gaa atc tgg ggt cca gat gct aac gac ttc aag cca 1344

His Arg Asp Pro Glu Ile Trp Gly Pro Asp Ala Asn Asp Phe Lys Pro

435 440 445

gaa aga ttc tct gaa ggt att tcc aag gct tgt aaa tac cca caa tct 1392

Glu Arg Phe Ser Glu Gly Ile Ser Lys Ala Cys Lys Tyr Pro Gln Ser

450 455 460

tac atc cca ttc ggt ttg ggt cca aga acc tgt gtc ggt aag aac ttc 1440

Tyr Ile Pro Phe Gly Leu Gly Pro Arg Thr Cys Val Gly Lys Asn Phe

465 470 475 480

ggt atg atg gaa gtc aaa gtt ttg gtt tct ttg att gtt tcc aag ttc 1488

Gly Met Met Glu Val Lys Val Leu Val Ser Leu Ile Val Ser Lys Phe

485 490 495

tct ttc acc ttg tct cca act tac caa cac tct cca tct cac aag ttg 1536

Ser Phe Thr Leu Ser Pro Thr Tyr Gln His Ser Pro Ser His Lys Leu

500 505 510

ttg gtt gaa cct caa cac ggt gtt gtc att aga gtc gtt 1575

Leu Val Glu Pro Gln His Gly Val Val Ile Arg Val Val

515 520 525

<210> 34

<211> 525

<212> PRT

<213> Arabidopsis thaliana

<400> 34

Met Glu Ser Leu Val Val His Thr Val Asn Ala Ile Trp Cys Ile Val

1 5 10 15

Ile Val Gly Ile Phe Ser Val Gly Tyr His Val Tyr Gly Arg Ala Val

20 25 30

Val Glu Gln Trp Arg Met Arg Arg Ser Leu Lys Leu Gln Gly Val Lys

35 40 45

Gly Pro Pro Pro Ser Ile Phe Asn Gly Asn Val Ser Glu Met Gln Arg

50 55 60

Ile Gln Ser Glu Ala Lys His Cys Ser Gly Asp Asn Ile Ile Ser His

65 70 75 80

Asp Tyr Ser Ser Ser Leu Phe Pro His Phe Asp His Trp Arg Lys Gln

85 90 95

Tyr Gly Arg Ile Tyr Thr Tyr Ser Thr Gly Leu Lys Gln His Leu Tyr

100 105 110

Ile Asn His Pro Glu Met Val Lys Glu Leu Ser Gln Thr Asn Thr Leu

115 120 125

Asn Leu Gly Arg Ile Thr His Ile Thr Lys Arg Leu Asn Pro Ile Leu

130 135 140

Gly Asn Gly Ile Ile Thr Ser Asn Gly Pro His Trp Ala His Gln Arg

145 150 155 160

Arg Ile Ile Ala Tyr Glu Phe Thr His Asp Lys Ile Lys Gly Met Val

165 170 175

Gly Leu Met Val Glu Ser Ala Met Pro Met Leu Asn Lys Trp Glu Glu

180 185 190

Met Val Lys Arg Gly Gly Glu Met Gly Cys Asp Ile Arg Val Asp Glu

195 200 205

Asp Leu Lys Asp Val Ser Ala Asp Val Ile Ala Lys Ala Cys Phe Gly

210 215 220

Ser Ser Phe Ser Lys Gly Lys Ala Ile Phe Ser Met Ile Arg Asp Leu

225 230 235 240

Leu Thr Ala Ile Thr Lys Arg Ser Val Leu Phe Arg Phe Asn Gly Phe

245 250 255

Thr Asp Met Val Phe Gly Ser Lys Lys His Gly Asp Val Asp Ile Asp

260 265 270

Ala Leu Glu Met Glu Leu Glu Ser Ser Ile Trp Glu Thr Val Lys Glu

275 280 285

Arg Glu Ile Glu Cys Lys Asp Thr His Lys Lys Asp Leu Met Gln Leu

290 295 300

Ile Leu Glu Gly Ala Met Arg Ser Cys Asp Gly Asn Leu Trp Asp Lys

305 310 315 320

Ser Ala Tyr Arg Arg Phe Val Val Asp Asn Cys Lys Ser Ile Tyr Phe

325 330 335

Ala Gly His Asp Ser Thr Ala Val Ser Val Ser Trp Cys Leu Met Leu

340 345 350

Leu Ala Leu Asn Pro Ser Trp Gln Val Lys Ile Arg Asp Glu Ile Leu

355 360 365

Ser Ser Cys Lys Asn Gly Ile Pro Asp Ala Glu Ser Ile Pro Asn Leu

370 375 380

Lys Thr Val Thr Met Val Ile Gln Glu Thr Met Arg Leu Tyr Pro Pro

385 390 395 400

Ala Pro Ile Val Gly Arg Glu Ala Ser Lys Asp Ile Arg Leu Gly Asp

405 410 415

Leu Val Val Pro Lys Gly Val Cys Ile Trp Thr Leu Ile Pro Ala Leu

420 425 430

His Arg Asp Pro Glu Ile Trp Gly Pro Asp Ala Asn Asp Phe Lys Pro

435 440 445

Glu Arg Phe Ser Glu Gly Ile Ser Lys Ala Cys Lys Tyr Pro Gln Ser

450 455 460

Tyr Ile Pro Phe Gly Leu Gly Pro Arg Thr Cys Val Gly Lys Asn Phe

465 470 475 480

Gly Met Met Glu Val Lys Val Leu Val Ser Leu Ile Val Ser Lys Phe

485 490 495

Ser Phe Thr Leu Ser Pro Thr Tyr Gln His Ser Pro Ser His Lys Leu

500 505 510

Leu Val Glu Pro Gln His Gly Val Val Ile Arg Val Val

515 520 525

<210> 35

<211> 1437

<212> DNA

<213> Ixeris dentata var. albiflora

<220>

<221> CDS

<222> (1)..(1437)

<400> 35

atg gat gcc gtt gcc gtc aac tct gaa acc atg tcc cac gtt gtt ttc 48

Met Asp Ala Val Ala Val Asn Ser Glu Thr Met Ser His Val Val Phe

1 5 10 15

att cca ttc cca gct caa tct cac atc aag tgt atg ttg aag ttg gcc 96

Ile Pro Phe Pro Ala Gln Ser His Ile Lys Cys Met Leu Lys Leu Ala

20 25 30

cgt cta tta cac cac aag ggt tta cac atc act ttc gtc aac act gaa 144

Arg Leu Leu His His Lys Gly Leu His Ile Thr Phe Val Asn Thr Glu

35 40 45

ttg aac cac aac caa ttg tta tct tct ggt ggt cca aac tct ttg gac 192

Leu Asn His Asn Gln Leu Leu Ser Ser Gly Gly Pro Asn Ser Leu Asp

50 55 60

ggt gaa cca ggt ttc aga ttc aag acc att cca gat ggt gtt cca gaa 240

Gly Glu Pro Gly Phe Arg Phe Lys Thr Ile Pro Asp Gly Val Pro Glu

65 70 75 80

ggt gct cca gac ttc atg tac gct tta tgt gac tct gtc ttg aac aaa 288

Gly Ala Pro Asp Phe Met Tyr Ala Leu Cys Asp Ser Val Leu Asn Lys

85 90 95

atg ttg gac cca ttt gtc gat ttg att ggt aga ttg gaa tct cca gcc 336

Met Leu Asp Pro Phe Val Asp Leu Ile Gly Arg Leu Glu Ser Pro Ala

100 105 110

acc tgt atc atc ggt gac ggt atg atg cct ttc act gtt gct gct gct 384

Thr Cys Ile Ile Gly Asp Gly Met Met Pro Phe Thr Val Ala Ala Ala

115 120 125

gaa aag ttg aaa ttg cca att atg cat ttc tgg act ttc cca gct gct 432

Glu Lys Leu Lys Leu Pro Ile Met His Phe Trp Thr Phe Pro Ala Ala

130 135 140

gct ttc ttg ggt tac tac caa gct cca aac ttg att gaa aag ggt ttc 480

Ala Phe Leu Gly Tyr Tyr Gln Ala Pro Asn Leu Ile Glu Lys Gly Phe

145 150 155 160

atc cca cct aag gac gaa tcc tgg tcc acc aac ggt tac ttg gaa acc 528

Ile Pro Pro Lys Asp Glu Ser Trp Ser Thr Asn Gly Tyr Leu Glu Thr

165 170 175

gtt gtt gac tcc att tct ggt ttg gaa ggt ttc aga atc aga gat atc 576

Val Val Asp Ser Ile Ser Gly Leu Glu Gly Phe Arg Ile Arg Asp Ile

180 185 190

cca gct tac ttc aga acc act gac cca aac gat tct gat ttc aac tac 624

Pro Ala Tyr Phe Arg Thr Thr Asp Pro Asn Asp Ser Asp Phe Asn Tyr

195 200 205

atc atc gaa tgt gtt aag gcc atc aga aag gtt tcc aac att gtc ttg 672

Ile Ile Glu Cys Val Lys Ala Ile Arg Lys Val Ser Asn Ile Val Leu

210 215 220

cac act ttc gaa gaa ttg gaa tct acc att atc aag gcc ttg caa cca 720

His Thr Phe Glu Glu Leu Glu Ser Thr Ile Ile Lys Ala Leu Gln Pro

225 230 235 240

atg atc cca cac gtc tac acc att ggt cca ttg gaa ttg ttg ttg aac 768

Met Ile Pro His Val Tyr Thr Ile Gly Pro Leu Glu Leu Leu Leu Asn

245 250 255

cca atc aag tta gaa gaa gaa act gaa aag ttg gac att aaa ggt tac 816

Pro Ile Lys Leu Glu Glu Glu Thr Glu Lys Leu Asp Ile Lys Gly Tyr

260 265 270

tct tta tgg aag gaa gat gac gaa tgt ttg aaa tgg ttg gac tcc aag 864

Ser Leu Trp Lys Glu Asp Asp Glu Cys Leu Lys Trp Leu Asp Ser Lys

275 280 285

gaa cca aac tcc gtc att tac gtc aac ttt ggt tct ttg atc tcc atg 912

Glu Pro Asn Ser Val Ile Tyr Val Asn Phe Gly Ser Leu Ile Ser Met

290 295 300

tcc aag gaa caa tta gct gaa ttt ggt tgg ggt ttg gtt aac tct aac 960

Ser Lys Glu Gln Leu Ala Glu Phe Gly Trp Gly Leu Val Asn Ser Asn

305 310 315 320

cac tgt ttc ttg tgg gtt atc aga aga gac ttg gtt gtc ggt gat tct 1008

His Cys Phe Leu Trp Val Ile Arg Arg Asp Leu Val Val Gly Asp Ser

325 330 335

gct cca ttg cct cca gaa ttg aaa gaa cgt atc aac gaa aga ggt ttc 1056

Ala Pro Leu Pro Pro Glu Leu Lys Glu Arg Ile Asn Glu Arg Gly Phe

340 345 350

atc gct tcc tgg tgt cca caa gaa aag gtc ttg aaa cat tcc tct gtt 1104

Ile Ala Ser Trp Cys Pro Gln Glu Lys Val Leu Lys His Ser Ser Val

355 360 365

ggt ggt ttc ttg act cac tgt ggt tgg ggt tcc atc atc gaa tct cta 1152

Gly Gly Phe Leu Thr His Cys Gly Trp Gly Ser Ile Ile Glu Ser Leu

370 375 380

tct gct ggt gtc cca atg ttg tgt tgg cca tac ttg tgg gac caa cca 1200

Ser Ala Gly Val Pro Met Leu Cys Trp Pro Tyr Leu Trp Asp Gln Pro

385 390 395 400

acc aac tgt cgt caa gct tgt aag gaa tgg gaa gtt ggt ttg gaa att 1248

Thr Asn Cys Arg Gln Ala Cys Lys Glu Trp Glu Val Gly Leu Glu Ile

405 410 415

gaa ggt aac gtt aac aag gat gaa gtc gaa aga ttg acc aga gaa ttg 1296

Glu Gly Asn Val Asn Lys Asp Glu Val Glu Arg Leu Thr Arg Glu Leu

420 425 430

atc ggt ggt gaa aag ggt aag caa atg aga tcc aag gct ttg gaa tgg 1344

Ile Gly Gly Glu Lys Gly Lys Gln Met Arg Ser Lys Ala Leu Glu Trp

435 440 445

aag aag aag atc gaa att gct act ggt cca aag ggt tct tct tct tta 1392

Lys Lys Lys Ile Glu Ile Ala Thr Gly Pro Lys Gly Ser Ser Ser Leu

450 455 460

aat gtc gaa aga ttg gct aac gac att aac atg ttc tcc aga aat 1437

Asn Val Glu Arg Leu Ala Asn Asp Ile Asn Met Phe Ser Arg Asn

465 470 475

<210> 36

<211> 479

<212> PRT

<213> Ixeris dentata var. albiflora

<400> 36

Met Asp Ala Val Ala Val Asn Ser Glu Thr Met Ser His Val Val Phe

1 5 10 15

Ile Pro Phe Pro Ala Gln Ser His Ile Lys Cys Met Leu Lys Leu Ala

20 25 30

Arg Leu Leu His His Lys Gly Leu His Ile Thr Phe Val Asn Thr Glu

35 40 45

Leu Asn His Asn Gln Leu Leu Ser Ser Gly Gly Pro Asn Ser Leu Asp

50 55 60

Gly Glu Pro Gly Phe Arg Phe Lys Thr Ile Pro Asp Gly Val Pro Glu

65 70 75 80

Gly Ala Pro Asp Phe Met Tyr Ala Leu Cys Asp Ser Val Leu Asn Lys

85 90 95

Met Leu Asp Pro Phe Val Asp Leu Ile Gly Arg Leu Glu Ser Pro Ala

100 105 110

Thr Cys Ile Ile Gly Asp Gly Met Met Pro Phe Thr Val Ala Ala Ala

115 120 125

Glu Lys Leu Lys Leu Pro Ile Met His Phe Trp Thr Phe Pro Ala Ala

130 135 140

Ala Phe Leu Gly Tyr Tyr Gln Ala Pro Asn Leu Ile Glu Lys Gly Phe

145 150 155 160

Ile Pro Pro Lys Asp Glu Ser Trp Ser Thr Asn Gly Tyr Leu Glu Thr

165 170 175

Val Val Asp Ser Ile Ser Gly Leu Glu Gly Phe Arg Ile Arg Asp Ile

180 185 190

Pro Ala Tyr Phe Arg Thr Thr Asp Pro Asn Asp Ser Asp Phe Asn Tyr

195 200 205

Ile Ile Glu Cys Val Lys Ala Ile Arg Lys Val Ser Asn Ile Val Leu

210 215 220

His Thr Phe Glu Glu Leu Glu Ser Thr Ile Ile Lys Ala Leu Gln Pro

225 230 235 240

Met Ile Pro His Val Tyr Thr Ile Gly Pro Leu Glu Leu Leu Leu Asn

245 250 255

Pro Ile Lys Leu Glu Glu Glu Thr Glu Lys Leu Asp Ile Lys Gly Tyr

260 265 270

Ser Leu Trp Lys Glu Asp Asp Glu Cys Leu Lys Trp Leu Asp Ser Lys

275 280 285

Glu Pro Asn Ser Val Ile Tyr Val Asn Phe Gly Ser Leu Ile Ser Met

290 295 300

Ser Lys Glu Gln Leu Ala Glu Phe Gly Trp Gly Leu Val Asn Ser Asn

305 310 315 320

His Cys Phe Leu Trp Val Ile Arg Arg Asp Leu Val Val Gly Asp Ser

325 330 335

Ala Pro Leu Pro Pro Glu Leu Lys Glu Arg Ile Asn Glu Arg Gly Phe

340 345 350

Ile Ala Ser Trp Cys Pro Gln Glu Lys Val Leu Lys His Ser Ser Val

355 360 365

Gly Gly Phe Leu Thr His Cys Gly Trp Gly Ser Ile Ile Glu Ser Leu

370 375 380

Ser Ala Gly Val Pro Met Leu Cys Trp Pro Tyr Leu Trp Asp Gln Pro

385 390 395 400

Thr Asn Cys Arg Gln Ala Cys Lys Glu Trp Glu Val Gly Leu Glu Ile

405 410 415

Glu Gly Asn Val Asn Lys Asp Glu Val Glu Arg Leu Thr Arg Glu Leu

420 425 430

Ile Gly Gly Glu Lys Gly Lys Gln Met Arg Ser Lys Ala Leu Glu Trp

435 440 445

Lys Lys Lys Ile Glu Ile Ala Thr Gly Pro Lys Gly Ser Ser Ser Leu

450 455 460

Asn Val Glu Arg Leu Ala Asn Asp Ile Asn Met Phe Ser Arg Asn

465 470 475

<210> 37

<211> 1446

<212> DNA

<213> Ricinus communis

<220>

<221> CDS

<222> (1)..(1446)

<400> 37

atg ggt tcc att gtc cgt gac cac gac aag cca cac gtt gtt tgt gtt 48

Met Gly Ser Ile Val Arg Asp His Asp Lys Pro His Val Val Cys Val

1 5 10 15

cca tac cca gct caa ggt cac gtt aac cca atg gtc aaa ttg gcc aag 96

Pro Tyr Pro Ala Gln Gly His Val Asn Pro Met Val Lys Leu Ala Lys

20 25 30

ttg ttg cac tac aac gat ttc cac gtc act ttc gtc aac act gaa tac 144

Leu Leu His Tyr Asn Asp Phe His Val Thr Phe Val Asn Thr Glu Tyr

35 40 45

aac cac aga aga tta ttg aac tcc aga ggt cct tct tct ttg gac ggt 192

Asn His Arg Arg Leu Leu Asn Ser Arg Gly Pro Ser Ser Leu Asp Gly

50 55 60

ttg cca gat ttc aga ttc gaa gcc atc tct gac ggt ttg cca cca tct 240

Leu Pro Asp Phe Arg Phe Glu Ala Ile Ser Asp Gly Leu Pro Pro Ser

65 70 75 80

gat gct aac gct acc caa gat atc cca tct cta tgt gac tct acc tcc 288

Asp Ala Asn Ala Thr Gln Asp Ile Pro Ser Leu Cys Asp Ser Thr Ser

85 90 95

aag aac tct ttg gct cca ttc aga aac ttg ttg ttg aag ttg aaa tcc 336

Lys Asn Ser Leu Ala Pro Phe Arg Asn Leu Leu Leu Lys Leu Lys Ser

100 105 110

tct gac tct ttg cca cca gtt acc tgt atc att tct gat gct tgt atg 384

Ser Asp Ser Leu Pro Pro Val Thr Cys Ile Ile Ser Asp Ala Cys Met

115 120 125

tcc ttc act ttg gat gct gct gaa gaa ttt ggt att cca gaa atc tta 432

Ser Phe Thr Leu Asp Ala Ala Glu Glu Phe Gly Ile Pro Glu Ile Leu

130 135 140

ttc tgg acc cca tct tct tgt ggt gtt ttg ggt tac tct caa tac cac 480

Phe Trp Thr Pro Ser Ser Cys Gly Val Leu Gly Tyr Ser Gln Tyr His

145 150 155 160

act ttg att gaa aag ggt ttg act cca tta aag gac gcc tct tac ttg 528

Thr Leu Ile Glu Lys Gly Leu Thr Pro Leu Lys Asp Ala Ser Tyr Leu

165 170 175

acc aac ggt tac ttg gaa acc act ttg gac tgg att cca ggt atg aag 576

Thr Asn Gly Tyr Leu Glu Thr Thr Leu Asp Trp Ile Pro Gly Met Lys

180 185 190

gat atc aga ttc aga gat ttg cca tct ttc atc aga acc act gac aga 624

Asp Ile Arg Phe Arg Asp Leu Pro Ser Phe Ile Arg Thr Thr Asp Arg

195 200 205

aac gat atc atg ttg aac ttt gtt gtc cgt gaa ttg gaa aga act tcc 672

Asn Asp Ile Met Leu Asn Phe Val Val Arg Glu Leu Glu Arg Thr Ser

210 215 220

aga gct tct gct gtt gtt ttc aac act ttc tac gcc ttc gaa aag gac 720

Arg Ala Ser Ala Val Val Phe Asn Thr Phe Tyr Ala Phe Glu Lys Asp

225 230 235 240

gtc tta gat gtc tta tcc acc atg ttc cca cca atc tac tcc atc ggt 768

Val Leu Asp Val Leu Ser Thr Met Phe Pro Pro Ile Tyr Ser Ile Gly

245 250 255

cca ttg caa ttg ttg gtt gac caa atc cca att gac aga aac ttg ggt 816

Pro Leu Gln Leu Leu Val Asp Gln Ile Pro Ile Asp Arg Asn Leu Gly

260 265 270

aac att ggt tcc aac tta tgg aag gaa caa cca gaa tgt att gac tgg 864

Asn Ile Gly Ser Asn Leu Trp Lys Glu Gln Pro Glu Cys Ile Asp Trp

275 280 285

ttg gac acc aag gaa cca aac tct gtt gtc tac gtc aac ttc ggt tcc 912

Leu Asp Thr Lys Glu Pro Asn Ser Val Val Tyr Val Asn Phe Gly Ser

290 295 300

atc act gtt atc act cct caa caa atg att gaa ttc gct tgg ggt cta 960

Ile Thr Val Ile Thr Pro Gln Gln Met Ile Glu Phe Ala Trp Gly Leu

305 310 315 320

gct tct tct aag aaa cca ttc tta tgg atc atc aga cca gac ttg gtt 1008

Ala Ser Ser Lys Lys Pro Phe Leu Trp Ile Ile Arg Pro Asp Leu Val

325 330 335

atc ggt gaa aac gct atg ttg cca gct gaa ttc gtt tct gaa acc aag 1056

Ile Gly Glu Asn Ala Met Leu Pro Ala Glu Phe Val Ser Glu Thr Lys

340 345 350

gat cgt ggt atg ttg gct tct tgg ggt cct caa gaa caa att ttg aaa 1104

Asp Arg Gly Met Leu Ala Ser Trp Gly Pro Gln Glu Gln Ile Leu Lys

355 360 365

cat cca gct gtc ggt ggt ttc ttg tct cac atg ggt tgg aac tcc act 1152

His Pro Ala Val Gly Gly Phe Leu Ser His Met Gly Trp Asn Ser Thr

370 375 380

ttg gac tcc atg tcc ggt ggt gtc cca atg gtt tgt tgg cca ttc ttt 1200

Leu Asp Ser Met Ser Gly Gly Val Pro Met Val Cys Trp Pro Phe Phe

385 390 395 400

gct gaa caa caa acc aac tgt aga ttt gct tgt acc gaa tgg ggt gtt 1248

Ala Glu Gln Gln Thr Asn Cys Arg Phe Ala Cys Thr Glu Trp Gly Val

405 410 415

ggt atg gaa att gac aac aat gtc aag aga gat gaa gtc aag aag ttg 1296

Gly Met Glu Ile Asp Asn Asn Val Lys Arg Asp Glu Val Lys Lys Leu

420 425 430

gtt gaa gtt ttg atg gac ggt aag aaa ggt aag gaa atg aag tcc aag 1344

Val Glu Val Leu Met Asp Gly Lys Lys Gly Lys Glu Met Lys Ser Lys

435 440 445

gcc atg gaa tgg aaa acc aag gct gaa gaa gct gcc aag cca ggt ggt 1392

Ala Met Glu Trp Lys Thr Lys Ala Glu Glu Ala Ala Lys Pro Gly Gly

450 455 460

tcc tct cat aac aac ttg gac aga tta gtc aag ttc atc aag ggt caa 1440

Ser Ser His Asn Asn Leu Asp Arg Leu Val Lys Phe Ile Lys Gly Gln

465 470 475 480

aag aat 1446

Lys Asn

<210> 38

<211> 482

<212> PRT

<213> Ricinus communis

<400> 38

Met Gly Ser Ile Val Arg Asp His Asp Lys Pro His Val Val Cys Val

1 5 10 15

Pro Tyr Pro Ala Gln Gly His Val Asn Pro Met Val Lys Leu Ala Lys

20 25 30

Leu Leu His Tyr Asn Asp Phe His Val Thr Phe Val Asn Thr Glu Tyr

35 40 45

Asn His Arg Arg Leu Leu Asn Ser Arg Gly Pro Ser Ser Leu Asp Gly

50 55 60

Leu Pro Asp Phe Arg Phe Glu Ala Ile Ser Asp Gly Leu Pro Pro Ser

65 70 75 80

Asp Ala Asn Ala Thr Gln Asp Ile Pro Ser Leu Cys Asp Ser Thr Ser

85 90 95

Lys Asn Ser Leu Ala Pro Phe Arg Asn Leu Leu Leu Lys Leu Lys Ser

100 105 110

Ser Asp Ser Leu Pro Pro Val Thr Cys Ile Ile Ser Asp Ala Cys Met

115 120 125

Ser Phe Thr Leu Asp Ala Ala Glu Glu Phe Gly Ile Pro Glu Ile Leu

130 135 140

Phe Trp Thr Pro Ser Ser Cys Gly Val Leu Gly Tyr Ser Gln Tyr His

145 150 155 160

Thr Leu Ile Glu Lys Gly Leu Thr Pro Leu Lys Asp Ala Ser Tyr Leu

165 170 175

Thr Asn Gly Tyr Leu Glu Thr Thr Leu Asp Trp Ile Pro Gly Met Lys

180 185 190

Asp Ile Arg Phe Arg Asp Leu Pro Ser Phe Ile Arg Thr Thr Asp Arg

195 200 205

Asn Asp Ile Met Leu Asn Phe Val Val Arg Glu Leu Glu Arg Thr Ser

210 215 220

Arg Ala Ser Ala Val Val Phe Asn Thr Phe Tyr Ala Phe Glu Lys Asp

225 230 235 240

Val Leu Asp Val Leu Ser Thr Met Phe Pro Pro Ile Tyr Ser Ile Gly

245 250 255

Pro Leu Gln Leu Leu Val Asp Gln Ile Pro Ile Asp Arg Asn Leu Gly

260 265 270

Asn Ile Gly Ser Asn Leu Trp Lys Glu Gln Pro Glu Cys Ile Asp Trp

275 280 285

Leu Asp Thr Lys Glu Pro Asn Ser Val Val Tyr Val Asn Phe Gly Ser

290 295 300

Ile Thr Val Ile Thr Pro Gln Gln Met Ile Glu Phe Ala Trp Gly Leu

305 310 315 320

Ala Ser Ser Lys Lys Pro Phe Leu Trp Ile Ile Arg Pro Asp Leu Val

325 330 335

Ile Gly Glu Asn Ala Met Leu Pro Ala Glu Phe Val Ser Glu Thr Lys

340 345 350

Asp Arg Gly Met Leu Ala Ser Trp Gly Pro Gln Glu Gln Ile Leu Lys

355 360 365

His Pro Ala Val Gly Gly Phe Leu Ser His Met Gly Trp Asn Ser Thr

370 375 380

Leu Asp Ser Met Ser Gly Gly Val Pro Met Val Cys Trp Pro Phe Phe

385 390 395 400

Ala Glu Gln Gln Thr Asn Cys Arg Phe Ala Cys Thr Glu Trp Gly Val

405 410 415

Gly Met Glu Ile Asp Asn Asn Val Lys Arg Asp Glu Val Lys Lys Leu

420 425 430

Val Glu Val Leu Met Asp Gly Lys Lys Gly Lys Glu Met Lys Ser Lys

435 440 445

Ala Met Glu Trp Lys Thr Lys Ala Glu Glu Ala Ala Lys Pro Gly Gly

450 455 460

Ser Ser His Asn Asn Leu Asp Arg Leu Val Lys Phe Ile Lys Gly Gln

465 470 475 480

Lys Asn

<210> 39

<211> 1374

<212> DNA

<213> Ixeris dentata var. albiflora

<220>

<221> CDS

<222> (1)..(1374)

<400> 39

atg gct gaa gaa cac aac aag acc aac aac tct tct cct cac gtt gtc 48

Met Ala Glu Glu His Asn Lys Thr Asn Asn Ser Ser Pro His Val Val

1 5 10 15

atc ttc cca ttc cca tct caa ggt cac att aac cca ttg att caa ttt 96

Ile Phe Pro Phe Pro Ser Gln Gly His Ile Asn Pro Leu Ile Gln Phe

20 25 30

gcc aag aga ttg tcc tcc aag ggt gtc aag cca act ttg atc act acc 144

Ala Lys Arg Leu Ser Ser Lys Gly Val Lys Pro Thr Leu Ile Thr Thr

35 40 45

atc tac att gct aag acc tct cca tac cca aac tct tcc att gtt gtt 192

Ile Tyr Ile Ala Lys Thr Ser Pro Tyr Pro Asn Ser Ser Ile Val Val

50 55 60

gaa cca att tct gat ggt ttc gac gac ggt ggt ttc aag tct gcc act 240

Glu Pro Ile Ser Asp Gly Phe Asp Asp Gly Gly Phe Lys Ser Ala Thr

65 70 75 80

tct gct gaa tct tac att gac act ttc cac caa gtt ggt tcc aaa tct 288

Ser Ala Glu Ser Tyr Ile Asp Thr Phe His Gln Val Gly Ser Lys Ser

85 90 95

cta gct aac ttg atc aga aag ttg gtc aac gaa ggt aac cat gtc gat 336

Leu Ala Asn Leu Ile Arg Lys Leu Val Asn Glu Gly Asn His Val Asp

100 105 110

gct atc atc tac gac tct ttc gtc acc tgg gct ttg gat gtt gct atg 384

Ala Ile Ile Tyr Asp Ser Phe Val Thr Trp Ala Leu Asp Val Ala Met

115 120 125

gaa tac ggt att gac ggt ggt tgt ttc ttc acc caa gct tgt gct gtc 432

Glu Tyr Gly Ile Asp Gly Gly Cys Phe Phe Thr Gln Ala Cys Ala Val

130 135 140

aac aac atc tac tac cat gtt tac aag ggt gtc ttg gaa att cca ttg 480

Asn Asn Ile Tyr Tyr His Val Tyr Lys Gly Val Leu Glu Ile Pro Leu

145 150 155 160

caa gct gct gct cca cca acc gtc act atc tta ttg cct gaa ttg cct 528

Gln Ala Ala Ala Pro Pro Thr Val Thr Ile Leu Leu Pro Glu Leu Pro

165 170 175

caa tta caa tta tgg gaa act cca tcc ttt gtc cac aac cca ggt cca 576

Gln Leu Gln Leu Trp Glu Thr Pro Ser Phe Val His Asn Pro Gly Pro

180 185 190

tac cca ggt tgg gct cac att gtt ttc aac caa ttc cca aac atc cac 624

Tyr Pro Gly Trp Ala His Ile Val Phe Asn Gln Phe Pro Asn Ile His

195 200 205

aac gcc aga tgg gtt ttc tcc aac act ttc ttc aaa ttg gaa gaa caa 672

Asn Ala Arg Trp Val Phe Ser Asn Thr Phe Phe Lys Leu Glu Glu Gln

210 215 220

gtt atc aaa tgg atg aga ttg atg tgg cca ttg atg gtt gtc ggt cca 720

Val Ile Lys Trp Met Arg Leu Met Trp Pro Leu Met Val Val Gly Pro

225 230 235 240

act gtt cca tcc atg tac ttg gac aag aga tta gaa gat gac gat gac 768

Thr Val Pro Ser Met Tyr Leu Asp Lys Arg Leu Glu Asp Asp Asp Asp

245 250 255

tac ggt atg tct ttg ttg aag cca aac cac att gaa tgt atg ggt tgg 816

Tyr Gly Met Ser Leu Leu Lys Pro Asn His Ile Glu Cys Met Gly Trp

260 265 270

ttg aat aac aaa cca aag ggt tcc gtt gtc tac gtt tcc ttc ggt tcc 864

Leu Asn Asn Lys Pro Lys Gly Ser Val Val Tyr Val Ser Phe Gly Ser

275 280 285

tac ggt gaa tta ggt gtt gct caa atg gaa gaa att gct tgg ggt ttg 912

Tyr Gly Glu Leu Gly Val Ala Gln Met Glu Glu Ile Ala Trp Gly Leu

290 295 300

aac gaa tct tcc gtc aac tat tta tgg gtt gtc aga gaa act gaa aag 960

Asn Glu Ser Ser Val Asn Tyr Leu Trp Val Val Arg Glu Thr Glu Lys

305 310 315 320

gaa aag ttg cca aag tct ttc ttg gct aat ggt ttg att gtt gaa tgg 1008

Glu Lys Leu Pro Lys Ser Phe Leu Ala Asn Gly Leu Ile Val Glu Trp

325 330 335

tgt cgt caa ttg gaa gtt ttg gct cac gaa gct gtc ggt tgt ttc gtc 1056

Cys Arg Gln Leu Glu Val Leu Ala His Glu Ala Val Gly Cys Phe Val

340 345 350

act cac tgt ggt ttc aac tcc tct ttg gaa acc atc tct ttg ggt gtc 1104

Thr His Cys Gly Phe Asn Ser Ser Leu Glu Thr Ile Ser Leu Gly Val

355 360 365

cca gtt gtt gcc atc cca caa tgg acc gat caa acc acc aac gct aag 1152

Pro Val Val Ala Ile Pro Gln Trp Thr Asp Gln Thr Thr Asn Ala Lys

370 375 380

tgt ttg gaa gat atc tgg ggt gtt ggt atc aga gcc aag act cca gtc 1200

Cys Leu Glu Asp Ile Trp Gly Val Gly Ile Arg Ala Lys Thr Pro Val

385 390 395 400

acc aga acc aac ttg gtc tgg tgt atc aag gaa atc atg gaa ggt gaa 1248

Thr Arg Thr Asn Leu Val Trp Cys Ile Lys Glu Ile Met Glu Gly Glu

405 410 415

cgt ggt gct gtt gct aga aag aac gcc atc aag tgg aag gac ttg gcc 1296

Arg Gly Ala Val Ala Arg Lys Asn Ala Ile Lys Trp Lys Asp Leu Ala

420 425 430

att gaa gct gtc tct cca ggt ggt tcc tct gac aag gac atc aac gaa 1344

Ile Glu Ala Val Ser Pro Gly Gly Ser Ser Asp Lys Asp Ile Asn Glu

435 440 445

ttt gtt tct caa ttg tct cca atc aaa tgt 1374

Phe Val Ser Gln Leu Ser Pro Ile Lys Cys

450 455

<210> 40

<211> 458

<212> PRT

<213> Ixeris dentata var. albiflora

<400> 40

Met Ala Glu Glu His Asn Lys Thr Asn Asn Ser Ser Pro His Val Val

1 5 10 15

Ile Phe Pro Phe Pro Ser Gln Gly His Ile Asn Pro Leu Ile Gln Phe

20 25 30

Ala Lys Arg Leu Ser Ser Lys Gly Val Lys Pro Thr Leu Ile Thr Thr

35 40 45

Ile Tyr Ile Ala Lys Thr Ser Pro Tyr Pro Asn Ser Ser Ile Val Val

50 55 60

Glu Pro Ile Ser Asp Gly Phe Asp Asp Gly Gly Phe Lys Ser Ala Thr

65 70 75 80

Ser Ala Glu Ser Tyr Ile Asp Thr Phe His Gln Val Gly Ser Lys Ser

85 90 95

Leu Ala Asn Leu Ile Arg Lys Leu Val Asn Glu Gly Asn His Val Asp

100 105 110

Ala Ile Ile Tyr Asp Ser Phe Val Thr Trp Ala Leu Asp Val Ala Met

115 120 125

Glu Tyr Gly Ile Asp Gly Gly Cys Phe Phe Thr Gln Ala Cys Ala Val

130 135 140

Asn Asn Ile Tyr Tyr His Val Tyr Lys Gly Val Leu Glu Ile Pro Leu

145 150 155 160

Gln Ala Ala Ala Pro Pro Thr Val Thr Ile Leu Leu Pro Glu Leu Pro

165 170 175

Gln Leu Gln Leu Trp Glu Thr Pro Ser Phe Val His Asn Pro Gly Pro

180 185 190

Tyr Pro Gly Trp Ala His Ile Val Phe Asn Gln Phe Pro Asn Ile His

195 200 205

Asn Ala Arg Trp Val Phe Ser Asn Thr Phe Phe Lys Leu Glu Glu Gln

210 215 220

Val Ile Lys Trp Met Arg Leu Met Trp Pro Leu Met Val Val Gly Pro

225 230 235 240

Thr Val Pro Ser Met Tyr Leu Asp Lys Arg Leu Glu Asp Asp Asp Asp

245 250 255

Tyr Gly Met Ser Leu Leu Lys Pro Asn His Ile Glu Cys Met Gly Trp

260 265 270

Leu Asn Asn Lys Pro Lys Gly Ser Val Val Tyr Val Ser Phe Gly Ser

275 280 285

Tyr Gly Glu Leu Gly Val Ala Gln Met Glu Glu Ile Ala Trp Gly Leu

290 295 300

Asn Glu Ser Ser Val Asn Tyr Leu Trp Val Val Arg Glu Thr Glu Lys

305 310 315 320

Glu Lys Leu Pro Lys Ser Phe Leu Ala Asn Gly Leu Ile Val Glu Trp

325 330 335

Cys Arg Gln Leu Glu Val Leu Ala His Glu Ala Val Gly Cys Phe Val

340 345 350

Thr His Cys Gly Phe Asn Ser Ser Leu Glu Thr Ile Ser Leu Gly Val

355 360 365

Pro Val Val Ala Ile Pro Gln Trp Thr Asp Gln Thr Thr Asn Ala Lys

370 375 380

Cys Leu Glu Asp Ile Trp Gly Val Gly Ile Arg Ala Lys Thr Pro Val

385 390 395 400

Thr Arg Thr Asn Leu Val Trp Cys Ile Lys Glu Ile Met Glu Gly Glu

405 410 415

Arg Gly Ala Val Ala Arg Lys Asn Ala Ile Lys Trp Lys Asp Leu Ala

420 425 430

Ile Glu Ala Val Ser Pro Gly Gly Ser Ser Asp Lys Asp Ile Asn Glu

435 440 445

Phe Val Ser Gln Leu Ser Pro Ile Lys Cys

450 455

<210> 41

<211> 1362

<212> DNA

<213> Populus trichocarpa

<220>

<221> CDS

<222> (1)..(1362)

<400> 41

atg gac aac aag aag tct cat gtc att gtc ttg acc tac cca gct caa 48

Met Asp Asn Lys Lys Ser His Val Ile Val Leu Thr Tyr Pro Ala Gln

1 5 10 15

ggt cac atc aac cca ttg ttg caa ttc gcc aag aga tta gct tcc aag 96

Gly His Ile Asn Pro Leu Leu Gln Phe Ala Lys Arg Leu Ala Ser Lys

20 25 30

ggt ttg aag gcc act ttg gct acc acc tac tac act gtt aac tcc att 144

Gly Leu Lys Ala Thr Leu Ala Thr Thr Tyr Tyr Thr Val Asn Ser Ile

35 40 45

gac gct cca act gtc ggt gtt gaa cca atc tct gat ggt ttc gat gaa 192

Asp Ala Pro Thr Val Gly Val Glu Pro Ile Ser Asp Gly Phe Asp Glu

50 55 60

ggt ggt ttc aag caa gct tcc tct cta gat gtc tac ttg gaa tct ttc 240

Gly Gly Phe Lys Gln Ala Ser Ser Leu Asp Val Tyr Leu Glu Ser Phe

65 70 75 80

aag acc gtc ggt tcc aga acc ttg act gaa tta gtc ttt aaa ttc aag 288

Lys Thr Val Gly Ser Arg Thr Leu Thr Glu Leu Val Phe Lys Phe Lys

85 90 95

gct tct ggt tct cct gtt aac tgt gtt gtt tac gac tcc atg ttg cca 336

Ala Ser Gly Ser Pro Val Asn Cys Val Val Tyr Asp Ser Met Leu Pro

100 105 110

tgg gct ttg gac gtt gcc aga gat ttg ggt atc tac gct gct gcc ttc 384

Trp Ala Leu Asp Val Ala Arg Asp Leu Gly Ile Tyr Ala Ala Ala Phe

115 120 125

atg acc acc tct gct tcc gtc tgt tcc atg tac tgg aga att gac tta 432

Met Thr Thr Ser Ala Ser Val Cys Ser Met Tyr Trp Arg Ile Asp Leu

130 135 140

ggt tta ttg tct ttg cca ttg aag caa caa acc gct acc gtt tct ttg 480

Gly Leu Leu Ser Leu Pro Leu Lys Gln Gln Thr Ala Thr Val Ser Leu

145 150 155 160

cca ggt ttg cca cca ttg ggt tgt tgt gat ttg cca tct ttc tta gct 528

Pro Gly Leu Pro Pro Leu Gly Cys Cys Asp Leu Pro Ser Phe Leu Ala

165 170 175

gaa cca act tct caa act gct tac ttg gaa gtt atc atg gaa aag ttc 576

Glu Pro Thr Ser Gln Thr Ala Tyr Leu Glu Val Ile Met Glu Lys Phe

180 185 190

cac tcc ttg aac gaa gat gac tgg gtt ttc tgt aac tct ttc gaa gat 624

His Ser Leu Asn Glu Asp Asp Trp Val Phe Cys Asn Ser Phe Glu Asp

195 200 205

ttg gaa att gaa ttg gtc aag gct atg aga ggt aaa tgg cca ttg gtt 672

Leu Glu Ile Glu Leu Val Lys Ala Met Arg Gly Lys Trp Pro Leu Val

210 215 220

atg gtt ggt cca atg gtt cca tct gcc tac ttg gac caa caa att gat 720

Met Val Gly Pro Met Val Pro Ser Ala Tyr Leu Asp Gln Gln Ile Asp

225 230 235 240

ggt gac cgt gcc tac ggt gct tct ttg tgg aaa cca act tct tct caa 768

Gly Asp Arg Ala Tyr Gly Ala Ser Leu Trp Lys Pro Thr Ser Ser Gln

245 250 255

tgt ttc acc tgg ttg gac act aag cct cca aga tct gtc atc tac gtt 816

Cys Phe Thr Trp Leu Asp Thr Lys Pro Pro Arg Ser Val Ile Tyr Val

260 265 270

tcc ttt ggt tcc atg ggt aac atc tcc gct gaa caa gtt gaa gaa att 864

Ser Phe Gly Ser Met Gly Asn Ile Ser Ala Glu Gln Val Glu Glu Ile

275 280 285

gct tgg ggt ttg aag gct tcc aac aga cca ttc tta tgg gtt atg aag 912

Ala Trp Gly Leu Lys Ala Ser Asn Arg Pro Phe Leu Trp Val Met Lys

290 295 300

gaa tct gaa aag aaa ttg cca act ggt ttc ttg aac tct gtc ggt gaa 960

Glu Ser Glu Lys Lys Leu Pro Thr Gly Phe Leu Asn Ser Val Gly Glu

305 310 315 320

acc ggt atg gtt gtt tcc tgg tgt aac caa ttg gaa gtc ttg gct cac 1008

Thr Gly Met Val Val Ser Trp Cys Asn Gln Leu Glu Val Leu Ala His

325 330 335

caa gct atc ggt tgt ttc gtc acc cac tgt ggt tgg aac tcc act ttg 1056

Gln Ala Ile Gly Cys Phe Val Thr His Cys Gly Trp Asn Ser Thr Leu

340 345 350

gaa ggt ttg ggt tta ggt gtt cca atg gtt tgt gtt act gaa aga tct 1104

Glu Gly Leu Gly Leu Gly Val Pro Met Val Cys Val Thr Glu Arg Ser

355 360 365

gac caa cca atg aac gct aag ttc gtt gaa gat gtc tgg aag gtc ggt 1152

Asp Gln Pro Met Asn Ala Lys Phe Val Glu Asp Val Trp Lys Val Gly

370 375 380

gtc aga gct aag aag gac gaa gtt ggt att gtc act aga gaa gaa ttg 1200

Val Arg Ala Lys Lys Asp Glu Val Gly Ile Val Thr Arg Glu Glu Leu

385 390 395 400

gaa aag tgt atc aga ggt gtc atg gac ggt gaa aac ggt gaa gaa atc 1248

Glu Lys Cys Ile Arg Gly Val Met Asp Gly Glu Asn Gly Glu Glu Ile

405 410 415

aag aga aac gcc aac aaa tgg aga gaa tta gct cgt tct gcc gtt tcc 1296

Lys Arg Asn Ala Asn Lys Trp Arg Glu Leu Ala Arg Ser Ala Val Ser

420 425 430

gtc ggt ggt tct tct gac atg aac atc aat gaa ttt gtt gtc aaa ttg 1344

Val Gly Gly Ser Ser Asp Met Asn Ile Asn Glu Phe Val Val Lys Leu

435 440 445

ttg gaa ggt aag aag ggg 1362

Leu Glu Gly Lys Lys Gly

450

<210> 42

<211> 454

<212> PRT

<213> Populus trichocarpa

<400> 42

Met Asp Asn Lys Lys Ser His Val Ile Val Leu Thr Tyr Pro Ala Gln

1 5 10 15

Gly His Ile Asn Pro Leu Leu Gln Phe Ala Lys Arg Leu Ala Ser Lys

20 25 30

Gly Leu Lys Ala Thr Leu Ala Thr Thr Tyr Tyr Thr Val Asn Ser Ile

35 40 45

Asp Ala Pro Thr Val Gly Val Glu Pro Ile Ser Asp Gly Phe Asp Glu

50 55 60

Gly Gly Phe Lys Gln Ala Ser Ser Leu Asp Val Tyr Leu Glu Ser Phe

65 70 75 80

Lys Thr Val Gly Ser Arg Thr Leu Thr Glu Leu Val Phe Lys Phe Lys

85 90 95

Ala Ser Gly Ser Pro Val Asn Cys Val Val Tyr Asp Ser Met Leu Pro

100 105 110

Trp Ala Leu Asp Val Ala Arg Asp Leu Gly Ile Tyr Ala Ala Ala Phe

115 120 125

Met Thr Thr Ser Ala Ser Val Cys Ser Met Tyr Trp Arg Ile Asp Leu

130 135 140

Gly Leu Leu Ser Leu Pro Leu Lys Gln Gln Thr Ala Thr Val Ser Leu

145 150 155 160

Pro Gly Leu Pro Pro Leu Gly Cys Cys Asp Leu Pro Ser Phe Leu Ala

165 170 175

Glu Pro Thr Ser Gln Thr Ala Tyr Leu Glu Val Ile Met Glu Lys Phe

180 185 190

His Ser Leu Asn Glu Asp Asp Trp Val Phe Cys Asn Ser Phe Glu Asp

195 200 205

Leu Glu Ile Glu Leu Val Lys Ala Met Arg Gly Lys Trp Pro Leu Val

210 215 220

Met Val Gly Pro Met Val Pro Ser Ala Tyr Leu Asp Gln Gln Ile Asp

225 230 235 240

Gly Asp Arg Ala Tyr Gly Ala Ser Leu Trp Lys Pro Thr Ser Ser Gln

245 250 255

Cys Phe Thr Trp Leu Asp Thr Lys Pro Pro Arg Ser Val Ile Tyr Val

260 265 270

Ser Phe Gly Ser Met Gly Asn Ile Ser Ala Glu Gln Val Glu Glu Ile

275 280 285

Ala Trp Gly Leu Lys Ala Ser Asn Arg Pro Phe Leu Trp Val Met Lys

290 295 300

Glu Ser Glu Lys Lys Leu Pro Thr Gly Phe Leu Asn Ser Val Gly Glu

305 310 315 320

Thr Gly Met Val Val Ser Trp Cys Asn Gln Leu Glu Val Leu Ala His

325 330 335

Gln Ala Ile Gly Cys Phe Val Thr His Cys Gly Trp Asn Ser Thr Leu

340 345 350

Glu Gly Leu Gly Leu Gly Val Pro Met Val Cys Val Thr Glu Arg Ser

355 360 365

Asp Gln Pro Met Asn Ala Lys Phe Val Glu Asp Val Trp Lys Val Gly

370 375 380

Val Arg Ala Lys Lys Asp Glu Val Gly Ile Val Thr Arg Glu Glu Leu

385 390 395 400

Glu Lys Cys Ile Arg Gly Val Met Asp Gly Glu Asn Gly Glu Glu Ile

405 410 415

Lys Arg Asn Ala Asn Lys Trp Arg Glu Leu Ala Arg Ser Ala Val Ser

420 425 430

Val Gly Gly Ser Ser Asp Met Asn Ile Asn Glu Phe Val Val Lys Leu

435 440 445

Leu Glu Gly Lys Lys Gly

450

<210> 43

<211> 1377

<212> DNA

<213> Nicotiana tabacum

<220>

<221> CDS

<222> (1)..(1377)

<400> 43

atg acc act caa aag gct cac tgt ttg atc tta cca tac cca gct caa 48

Met Thr Thr Gln Lys Ala His Cys Leu Ile Leu Pro Tyr Pro Ala Gln

1 5 10 15

ggt cac atc aac cca atg ttg caa ttt tcc aag aga tta caa tct aag 96

Gly His Ile Asn Pro Met Leu Gln Phe Ser Lys Arg Leu Gln Ser Lys

20 25 30

ggt gtc aag atc acc att gct gct acc aag tct ttc tta aag acc atg 144

Gly Val Lys Ile Thr Ile Ala Ala Thr Lys Ser Phe Leu Lys Thr Met

35 40 45

caa gaa ttg tcc act tct gtc tct gtt gaa gct atc tct gat ggt tac 192

Gln Glu Leu Ser Thr Ser Val Ser Val Glu Ala Ile Ser Asp Gly Tyr

50 55 60

gat gac ggt ggt aga gaa caa gct ggt act ttc gtt gct tac atc acc 240

Asp Asp Gly Gly Arg Glu Gln Ala Gly Thr Phe Val Ala Tyr Ile Thr

65 70 75 80

aga ttc aag gaa gtt ggt tct gac act cta tcc caa tta atc ggt aag 288

Arg Phe Lys Glu Val Gly Ser Asp Thr Leu Ser Gln Leu Ile Gly Lys

85 90 95

ttg acc aac tgt ggt tgt cca gtt tct tgt atc gtc tac gat cct ttc 336

Leu Thr Asn Cys Gly Cys Pro Val Ser Cys Ile Val Tyr Asp Pro Phe

100 105 110

ttg cca tgg gct gtc gaa gtc ggt aac aac ttt ggt gtt gcc act gct 384

Leu Pro Trp Ala Val Glu Val Gly Asn Asn Phe Gly Val Ala Thr Ala

115 120 125

gct ttc ttc act caa tct tgt gct gtt gac aac atc tac tac cat gtc 432

Ala Phe Phe Thr Gln Ser Cys Ala Val Asp Asn Ile Tyr Tyr His Val

130 135 140

cac aaa ggt gtt ttg aaa ttg cca cca act gat gtt gac aag gaa att 480

His Lys Gly Val Leu Lys Leu Pro Pro Thr Asp Val Asp Lys Glu Ile

145 150 155 160

tcc att cca ggt ttg ttg acc att gaa gct tct gat gtt cca tct ttc 528

Ser Ile Pro Gly Leu Leu Thr Ile Glu Ala Ser Asp Val Pro Ser Phe

165 170 175

gtt tcc aac cca gaa tct tcc aga atc ttg gaa atg ttg gtc aac caa 576

Val Ser Asn Pro Glu Ser Ser Arg Ile Leu Glu Met Leu Val Asn Gln

180 185 190

ttc tcc aat ttg gaa aac act gac tgg gtt ttg atc aac tct ttc tac 624

Phe Ser Asn Leu Glu Asn Thr Asp Trp Val Leu Ile Asn Ser Phe Tyr

195 200 205

gaa ttg gaa aag gaa gtc att gac tgg atg gcc aag atc tac cca atc 672

Glu Leu Glu Lys Glu Val Ile Asp Trp Met Ala Lys Ile Tyr Pro Ile

210 215 220

aag acc att ggt cca acc att cca tcc atg tac ttg gac aaa aga tta 720

Lys Thr Ile Gly Pro Thr Ile Pro Ser Met Tyr Leu Asp Lys Arg Leu

225 230 235 240

cca gat gac aag gaa tac ggt ttg tcc gtc ttt aaa cca atg acc aat 768

Pro Asp Asp Lys Glu Tyr Gly Leu Ser Val Phe Lys Pro Met Thr Asn

245 250 255

gct tgt ttg aac tgg ttg aac cac caa cca gtt tct tcc gtt gtc tac 816

Ala Cys Leu Asn Trp Leu Asn His Gln Pro Val Ser Ser Val Val Tyr

260 265 270

gtt tct ttc ggt tct ttg gct aag ttg gaa gct gaa caa atg gaa gaa 864

Val Ser Phe Gly Ser Leu Ala Lys Leu Glu Ala Glu Gln Met Glu Glu

275 280 285

ttg gct tgg ggt ttg tcc aac tcc aac aag aac ttc tta tgg gtt gtc 912

Leu Ala Trp Gly Leu Ser Asn Ser Asn Lys Asn Phe Leu Trp Val Val

290 295 300

aga tct act gaa gaa tcc aaa ttg cca aac aac ttc ttg gaa gaa ttg 960

Arg Ser Thr Glu Glu Ser Lys Leu Pro Asn Asn Phe Leu Glu Glu Leu

305 310 315 320

gct tct gaa aag ggt ttg gtt gtt tcc tgg tgt cca caa tta caa gtt 1008

Ala Ser Glu Lys Gly Leu Val Val Ser Trp Cys Pro Gln Leu Gln Val

325 330 335

ttg gaa cac aag tcc atc ggt tgt ttc ttg act cac tgt ggt tgg aac 1056

Leu Glu His Lys Ser Ile Gly Cys Phe Leu Thr His Cys Gly Trp Asn

340 345 350

tct act ttg gaa gcc atc tct ttg ggt gtt cca atg att gcc atg cct 1104

Ser Thr Leu Glu Ala Ile Ser Leu Gly Val Pro Met Ile Ala Met Pro

355 360 365

cac tgg tct gac caa cca acc aac gcc aag ttg gtc gaa gat gtc tgg 1152

His Trp Ser Asp Gln Pro Thr Asn Ala Lys Leu Val Glu Asp Val Trp

370 375 380

gaa atg ggt atc aga cca aag caa gat gaa aag ggt tta gtc cgt cgt 1200

Glu Met Gly Ile Arg Pro Lys Gln Asp Glu Lys Gly Leu Val Arg Arg

385 390 395 400

gaa gtc att gaa gaa tgt atc aag att gtc atg gaa gaa aag aag ggt 1248

Glu Val Ile Glu Glu Cys Ile Lys Ile Val Met Glu Glu Lys Lys Gly

405 410 415

aag aag atc aga gaa aac gcc aag aaa tgg aag gaa ttg gcc aga aag 1296

Lys Lys Ile Arg Glu Asn Ala Lys Lys Trp Lys Glu Leu Ala Arg Lys

420 425 430

gct gtt gac gaa ggt ggt tct tct gac aga aac att gaa gaa ttc gtt 1344

Ala Val Asp Glu Gly Gly Ser Ser Asp Arg Asn Ile Glu Glu Phe Val

435 440 445

tcc aaa ttg gtc acc att gct tct gtc gag agt 1377

Ser Lys Leu Val Thr Ile Ala Ser Val Glu Ser

450 455

<210> 44

<211> 459

<212> PRT

<213> Nicotiana tabacum

<400> 44

Met Thr Thr Gln Lys Ala His Cys Leu Ile Leu Pro Tyr Pro Ala Gln

1 5 10 15

Gly His Ile Asn Pro Met Leu Gln Phe Ser Lys Arg Leu Gln Ser Lys

20 25 30

Gly Val Lys Ile Thr Ile Ala Ala Thr Lys Ser Phe Leu Lys Thr Met

35 40 45

Gln Glu Leu Ser Thr Ser Val Ser Val Glu Ala Ile Ser Asp Gly Tyr

50 55 60

Asp Asp Gly Gly Arg Glu Gln Ala Gly Thr Phe Val Ala Tyr Ile Thr

65 70 75 80

Arg Phe Lys Glu Val Gly Ser Asp Thr Leu Ser Gln Leu Ile Gly Lys

85 90 95

Leu Thr Asn Cys Gly Cys Pro Val Ser Cys Ile Val Tyr Asp Pro Phe

100 105 110

Leu Pro Trp Ala Val Glu Val Gly Asn Asn Phe Gly Val Ala Thr Ala

115 120 125

Ala Phe Phe Thr Gln Ser Cys Ala Val Asp Asn Ile Tyr Tyr His Val

130 135 140

His Lys Gly Val Leu Lys Leu Pro Pro Thr Asp Val Asp Lys Glu Ile

145 150 155 160

Ser Ile Pro Gly Leu Leu Thr Ile Glu Ala Ser Asp Val Pro Ser Phe

165 170 175

Val Ser Asn Pro Glu Ser Ser Arg Ile Leu Glu Met Leu Val Asn Gln

180 185 190

Phe Ser Asn Leu Glu Asn Thr Asp Trp Val Leu Ile Asn Ser Phe Tyr

195 200 205

Glu Leu Glu Lys Glu Val Ile Asp Trp Met Ala Lys Ile Tyr Pro Ile

210 215 220

Lys Thr Ile Gly Pro Thr Ile Pro Ser Met Tyr Leu Asp Lys Arg Leu

225 230 235 240

Pro Asp Asp Lys Glu Tyr Gly Leu Ser Val Phe Lys Pro Met Thr Asn

245 250 255

Ala Cys Leu Asn Trp Leu Asn His Gln Pro Val Ser Ser Val Val Tyr

260 265 270

Val Ser Phe Gly Ser Leu Ala Lys Leu Glu Ala Glu Gln Met Glu Glu

275 280 285

Leu Ala Trp Gly Leu Ser Asn Ser Asn Lys Asn Phe Leu Trp Val Val

290 295 300

Arg Ser Thr Glu Glu Ser Lys Leu Pro Asn Asn Phe Leu Glu Glu Leu

305 310 315 320

Ala Ser Glu Lys Gly Leu Val Val Ser Trp Cys Pro Gln Leu Gln Val

325 330 335

Leu Glu His Lys Ser Ile Gly Cys Phe Leu Thr His Cys Gly Trp Asn

340 345 350

Ser Thr Leu Glu Ala Ile Ser Leu Gly Val Pro Met Ile Ala Met Pro

355 360 365

His Trp Ser Asp Gln Pro Thr Asn Ala Lys Leu Val Glu Asp Val Trp

370 375 380

Glu Met Gly Ile Arg Pro Lys Gln Asp Glu Lys Gly Leu Val Arg Arg

385 390 395 400

Glu Val Ile Glu Glu Cys Ile Lys Ile Val Met Glu Glu Lys Lys Gly

405 410 415

Lys Lys Ile Arg Glu Asn Ala Lys Lys Trp Lys Glu Leu Ala Arg Lys

420 425 430

Ala Val Asp Glu Gly Gly Ser Ser Asp Arg Asn Ile Glu Glu Phe Val

435 440 445

Ser Lys Leu Val Thr Ile Ala Ser Val Glu Ser

450 455

<210> 45

<211> 1434

<212> DNA

<213> Vaccaria hispanica

<220>

<221> CDS

<222> (1)..(1434)

<400> 45

atg tcc aac aat gaa aac aat gcc act caa gtt atc gtt ttg cca tac 48

Met Ser Asn Asn Glu Asn Asn Ala Thr Gln Val Ile Val Leu Pro Tyr

1 5 10 15

cac ggt caa ggt cac atg aac acc atg gtt caa ttt gcc aag aga tta 96

His Gly Gln Gly His Met Asn Thr Met Val Gln Phe Ala Lys Arg Leu

20 25 30

gct tgg aag ggt gtc cac gtc acc att gct acc act ttc aac act atc 144

Ala Trp Lys Gly Val His Val Thr Ile Ala Thr Thr Phe Asn Thr Ile

35 40 45

caa caa atg aaa ttg aac att tct tct tac aac tcc atc act ttg gaa 192

Gln Gln Met Lys Leu Asn Ile Ser Ser Tyr Asn Ser Ile Thr Leu Glu

50 55 60

cca atc tac gat gac act gat gac tcc act ttg cac atc aag gac aga 240

Pro Ile Tyr Asp Asp Thr Asp Asp Ser Thr Leu His Ile Lys Asp Arg

65 70 75 80

atg gcc aga ttt gaa gct gaa gcc gct tcc aat ttg acc aga gtt ttg 288

Met Ala Arg Phe Glu Ala Glu Ala Ala Ser Asn Leu Thr Arg Val Leu

85 90 95

gaa gcc aag aaa caa caa caa gct tta aac aag aag tgt ttg ttg gtt 336

Glu Ala Lys Lys Gln Gln Gln Ala Leu Asn Lys Lys Cys Leu Leu Val

100 105 110

tac cac ggt tct ttg aac tgg gct tta gtc gtt gct cac caa caa aac 384

Tyr His Gly Ser Leu Asn Trp Ala Leu Val Val Ala His Gln Gln Asn

115 120 125

gtt gct ggt gct gct ttc ttc acc gct gct tct gct tct ttc gct tgt 432

Val Ala Gly Ala Ala Phe Phe Thr Ala Ala Ser Ala Ser Phe Ala Cys

130 135 140

tac tac tac ttg cat ttg gaa tct caa ggt aag ggt gtc gac ttg gaa 480

Tyr Tyr Tyr Leu His Leu Glu Ser Gln Gly Lys Gly Val Asp Leu Glu

145 150 155 160

gaa tta cca tcc atc tta cca cca cca aag gtc att gtt caa aag ttg 528

Glu Leu Pro Ser Ile Leu Pro Pro Pro Lys Val Ile Val Gln Lys Leu

165 170 175

cca aag tct ttc tta gct tac ggt gac aac aac tct cac aac aac aac 576

Pro Lys Ser Phe Leu Ala Tyr Gly Asp Asn Asn Ser His Asn Asn Asn

180 185 190

aac aac aac aac aac aac aac aac aac aac aac atg ggt ttg cac cca 624

Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Met Gly Leu His Pro

195 200 205

ttg gtc ttg tgg ttg ttg aaa gat tac ggt aac tcc gtc aag gct gac 672

Leu Val Leu Trp Leu Leu Lys Asp Tyr Gly Asn Ser Val Lys Ala Asp

210 215 220

ttc gtt ttg ttg aac tct ttc gac aaa ttg gaa gaa gaa gct atc aaa 720

Phe Val Leu Leu Asn Ser Phe Asp Lys Leu Glu Glu Glu Ala Ile Lys

225 230 235 240

tgg atc tct aac atc tgt tcc gtc aag act atc ggt cca acc att cca 768

Trp Ile Ser Asn Ile Cys Ser Val Lys Thr Ile Gly Pro Thr Ile Pro

245 250 255

tct acc tac ttg gac aag caa att gaa aac gat gtt gac tac ggt ttc 816

Ser Thr Tyr Leu Asp Lys Gln Ile Glu Asn Asp Val Asp Tyr Gly Phe

260 265 270

aac caa tac aag cca acc aat gaa gat tgt atg aaa tgg ttg gac acc 864

Asn Gln Tyr Lys Pro Thr Asn Glu Asp Cys Met Lys Trp Leu Asp Thr

275 280 285

aag gaa gcc aac tcc gtt gtc tac atc gcc ttc ggt tct gtt gct cgt 912

Lys Glu Ala Asn Ser Val Val Tyr Ile Ala Phe Gly Ser Val Ala Arg

290 295 300

ttg tct gtc gaa caa atg gct gaa att gct aag gct ttg gac cat tct 960

Leu Ser Val Glu Gln Met Ala Glu Ile Ala Lys Ala Leu Asp His Ser

305 310 315 320

tcc aag tct ttc atc tgg gtt gtc aga gaa act gaa aag gaa aag ttg 1008

Ser Lys Ser Phe Ile Trp Val Val Arg Glu Thr Glu Lys Glu Lys Leu

325 330 335

cca gtc gat ttg gtt gaa aag atc tct ggt caa ggt atg gtt gtc cca 1056

Pro Val Asp Leu Val Glu Lys Ile Ser Gly Gln Gly Met Val Val Pro

340 345 350

tgg gct cct caa ttg gaa gtt ttg gct cac gat gct gtc ggt tgt ttc 1104

Trp Ala Pro Gln Leu Glu Val Leu Ala His Asp Ala Val Gly Cys Phe

355 360 365

gtt tct cac tgt ggt tgg aac tcc acc att gaa gct ttg tct ttc ggt 1152

Val Ser His Cys Gly Trp Asn Ser Thr Ile Glu Ala Leu Ser Phe Gly

370 375 380

gtt cca att ttg gcc atg cct caa ttc tta gat caa ttg gtc gat gct 1200

Val Pro Ile Leu Ala Met Pro Gln Phe Leu Asp Gln Leu Val Asp Ala

385 390 395 400

cat ttc gtt gac aga gtc tgg ggt gtc ggt att gct cca act gtc gat 1248

His Phe Val Asp Arg Val Trp Gly Val Gly Ile Ala Pro Thr Val Asp

405 410 415

gaa aac gat ttg gtt act caa gaa gaa atc tcc aga tgt cta gac gaa 1296

Glu Asn Asp Leu Val Thr Gln Glu Glu Ile Ser Arg Cys Leu Asp Glu

420 425 430

atg atg ggt ggt ggt cca gaa ggt gaa aag atc aag aag aac gtt gcc 1344

Met Met Gly Gly Gly Pro Glu Gly Glu Lys Ile Lys Lys Asn Val Ala

435 440 445

atg tgg aag gaa ttg acc aag gaa gct ttg gac aag ggt ggt tcc tct 1392

Met Trp Lys Glu Leu Thr Lys Glu Ala Leu Asp Lys Gly Gly Ser Ser

450 455 460

gac aag cac att gac gaa atc att gaa tgg tta tct tcc tcc 1434

Asp Lys His Ile Asp Glu Ile Ile Glu Trp Leu Ser Ser Ser

465 470 475

<210> 46

<211> 478

<212> PRT

<213> Vaccaria hispanica

<400> 46

Met Ser Asn Asn Glu Asn Asn Ala Thr Gln Val Ile Val Leu Pro Tyr

1 5 10 15

His Gly Gln Gly His Met Asn Thr Met Val Gln Phe Ala Lys Arg Leu

20 25 30

Ala Trp Lys Gly Val His Val Thr Ile Ala Thr Thr Phe Asn Thr Ile

35 40 45

Gln Gln Met Lys Leu Asn Ile Ser Ser Tyr Asn Ser Ile Thr Leu Glu

50 55 60

Pro Ile Tyr Asp Asp Thr Asp Asp Ser Thr Leu His Ile Lys Asp Arg

65 70 75 80

Met Ala Arg Phe Glu Ala Glu Ala Ala Ser Asn Leu Thr Arg Val Leu

85 90 95

Glu Ala Lys Lys Gln Gln Gln Ala Leu Asn Lys Lys Cys Leu Leu Val

100 105 110

Tyr His Gly Ser Leu Asn Trp Ala Leu Val Val Ala His Gln Gln Asn

115 120 125

Val Ala Gly Ala Ala Phe Phe Thr Ala Ala Ser Ala Ser Phe Ala Cys

130 135 140

Tyr Tyr Tyr Leu His Leu Glu Ser Gln Gly Lys Gly Val Asp Leu Glu

145 150 155 160

Glu Leu Pro Ser Ile Leu Pro Pro Pro Lys Val Ile Val Gln Lys Leu

165 170 175

Pro Lys Ser Phe Leu Ala Tyr Gly Asp Asn Asn Ser His Asn Asn Asn

180 185 190

Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Met Gly Leu His Pro

195 200 205

Leu Val Leu Trp Leu Leu Lys Asp Tyr Gly Asn Ser Val Lys Ala Asp

210 215 220

Phe Val Leu Leu Asn Ser Phe Asp Lys Leu Glu Glu Glu Ala Ile Lys

225 230 235 240

Trp Ile Ser Asn Ile Cys Ser Val Lys Thr Ile Gly Pro Thr Ile Pro

245 250 255

Ser Thr Tyr Leu Asp Lys Gln Ile Glu Asn Asp Val Asp Tyr Gly Phe

260 265 270

Asn Gln Tyr Lys Pro Thr Asn Glu Asp Cys Met Lys Trp Leu Asp Thr

275 280 285

Lys Glu Ala Asn Ser Val Val Tyr Ile Ala Phe Gly Ser Val Ala Arg

290 295 300

Leu Ser Val Glu Gln Met Ala Glu Ile Ala Lys Ala Leu Asp His Ser

305 310 315 320

Ser Lys Ser Phe Ile Trp Val Val Arg Glu Thr Glu Lys Glu Lys Leu

325 330 335

Pro Val Asp Leu Val Glu Lys Ile Ser Gly Gln Gly Met Val Val Pro

340 345 350

Trp Ala Pro Gln Leu Glu Val Leu Ala His Asp Ala Val Gly Cys Phe

355 360 365

Val Ser His Cys Gly Trp Asn Ser Thr Ile Glu Ala Leu Ser Phe Gly

370 375 380

Val Pro Ile Leu Ala Met Pro Gln Phe Leu Asp Gln Leu Val Asp Ala

385 390 395 400

His Phe Val Asp Arg Val Trp Gly Val Gly Ile Ala Pro Thr Val Asp

405 410 415

Glu Asn Asp Leu Val Thr Gln Glu Glu Ile Ser Arg Cys Leu Asp Glu

420 425 430

Met Met Gly Gly Gly Pro Glu Gly Glu Lys Ile Lys Lys Asn Val Ala

435 440 445

Met Trp Lys Glu Leu Thr Lys Glu Ala Leu Asp Lys Gly Gly Ser Ser

450 455 460

Asp Lys His Ile Asp Glu Ile Ile Glu Trp Leu Ser Ser Ser

465 470 475

<210> 47

<211> 1443

<212> DNA

<213> Streptococcus mutans

<220>

<221> CDS

<222> (1)..(1443)

<400> 47

atg cca atc atc aac aag acc atg ttg atc act tac gct gac tct cta 48

Met Pro Ile Ile Asn Lys Thr Met Leu Ile Thr Tyr Ala Asp Ser Leu

1 5 10 15

ggt aag aac ttg aaa gaa ttg aat gaa aac att gaa aac tac ttc ggt 96

Gly Lys Asn Leu Lys Glu Leu Asn Glu Asn Ile Glu Asn Tyr Phe Gly

20 25 30

gat gct gtc ggt ggt gtt cac ttg ttg cct ttc ttc cca tcc acc ggt 144

Asp Ala Val Gly Gly Val His Leu Leu Pro Phe Phe Pro Ser Thr Gly

35 40 45

gac aga ggt ttc gct cca att gac tac cat gaa gtc gac tcc gcc ttc 192

Asp Arg Gly Phe Ala Pro Ile Asp Tyr His Glu Val Asp Ser Ala Phe

50 55 60

ggt gac tgg gat gac gtt aag tgt ttg ggt gaa aag tac tac ttg atg 240

Gly Asp Trp Asp Asp Val Lys Cys Leu Gly Glu Lys Tyr Tyr Leu Met

65 70 75 80

ttt gac ttc atg atc aac cac atc tcc aga caa tct aag tac tac aag 288

Phe Asp Phe Met Ile Asn His Ile Ser Arg Gln Ser Lys Tyr Tyr Lys

85 90 95

gac tac caa gaa aag cac gaa gct tct gct tac aag gat ttg ttc ttg 336

Asp Tyr Gln Glu Lys His Glu Ala Ser Ala Tyr Lys Asp Leu Phe Leu

100 105 110

aac tgg gac aaa ttc tgg cca aag aac aga cca act caa gaa gat gtc 384

Asn Trp Asp Lys Phe Trp Pro Lys Asn Arg Pro Thr Gln Glu Asp Val

115 120 125

gac ttg atc tac aag aga aag gac cgt gcc cca aag caa gaa atc caa 432

Asp Leu Ile Tyr Lys Arg Lys Asp Arg Ala Pro Lys Gln Glu Ile Gln

130 135 140

ttt gcc gat ggt tct gtt gaa cac tta tgg aac act ttc ggt gaa gaa 480

Phe Ala Asp Gly Ser Val Glu His Leu Trp Asn Thr Phe Gly Glu Glu

145 150 155 160

caa atc gat ttg gat gtc acc aag gaa gtt acc atg gac ttc atc aga 528

Gln Ile Asp Leu Asp Val Thr Lys Glu Val Thr Met Asp Phe Ile Arg

165 170 175

tcc acc att gaa aac ttg gct gct aac ggt tgt gac ttg atc aga ttg 576

Ser Thr Ile Glu Asn Leu Ala Ala Asn Gly Cys Asp Leu Ile Arg Leu

180 185 190

gat gct ttc gct tac gct gtt aag aag cta gac acc aac gat ttc ttc 624

Asp Ala Phe Ala Tyr Ala Val Lys Lys Leu Asp Thr Asn Asp Phe Phe

195 200 205

gtc gaa cca gaa atc tgg act ttg ttg gac aag gtc cgt gac att gct 672

Val Glu Pro Glu Ile Trp Thr Leu Leu Asp Lys Val Arg Asp Ile Ala

210 215 220

gct gtt tct ggt gct gaa att ttg cca gaa att cac gaa cat tac act 720

Ala Val Ser Gly Ala Glu Ile Leu Pro Glu Ile His Glu His Tyr Thr

225 230 235 240

atc caa ttc aag att gct gac cac gac tac tac gtt tac gac ttt gct 768

Ile Gln Phe Lys Ile Ala Asp His Asp Tyr Tyr Val Tyr Asp Phe Ala

245 250 255

ttg cca atg gtt act tta tac tct ttg tac tcc tcc aag gtc gac aga 816

Leu Pro Met Val Thr Leu Tyr Ser Leu Tyr Ser Ser Lys Val Asp Arg

260 265 270

tta gct aaa tgg ttg aaa atg tct cca atg aag caa ttc acc act ttg 864

Leu Ala Lys Trp Leu Lys Met Ser Pro Met Lys Gln Phe Thr Thr Leu

275 280 285

gac acc cac gac ggt att ggt gtt gtc gat gtc aag gat atc ttg acc 912

Asp Thr His Asp Gly Ile Gly Val Val Asp Val Lys Asp Ile Leu Thr

290 295 300

gat gaa gaa atc act tac act tcc aat gaa tta tac aag gtc ggt gcc 960

Asp Glu Glu Ile Thr Tyr Thr Ser Asn Glu Leu Tyr Lys Val Gly Ala

305 310 315 320

aac gtt aac aga aaa tac tct act gct gaa tac aac aac ttg gat atc 1008

Asn Val Asn Arg Lys Tyr Ser Thr Ala Glu Tyr Asn Asn Leu Asp Ile

325 330 335

tac caa atc aac tct acc tac tac tct gct ttg ggt gac gat gac caa 1056

Tyr Gln Ile Asn Ser Thr Tyr Tyr Ser Ala Leu Gly Asp Asp Asp Gln

340 345 350

aag tat ttc ttg gcc aga tta atc caa gct ttc gct cca ggt atc cca 1104

Lys Tyr Phe Leu Ala Arg Leu Ile Gln Ala Phe Ala Pro Gly Ile Pro

355 360 365

caa gtc tac tac gtt ggt ttc ttg gcc ggt aag aac gat ttg gaa ttg 1152

Gln Val Tyr Tyr Val Gly Phe Leu Ala Gly Lys Asn Asp Leu Glu Leu

370 375 380

ttg gaa tcc acc aag gaa ggt aga aac atc aac aga cac tac tac tct 1200

Leu Glu Ser Thr Lys Glu Gly Arg Asn Ile Asn Arg His Tyr Tyr Ser

385 390 395 400

tct gaa gaa att gct aag gaa gtc aaa aga cca gtt gtt aag gct ttg 1248

Ser Glu Glu Ile Ala Lys Glu Val Lys Arg Pro Val Val Lys Ala Leu

405 410 415

ttg aac tta ttc act tac aga aac caa tct gct gcc ttt gac ttg gat 1296

Leu Asn Leu Phe Thr Tyr Arg Asn Gln Ser Ala Ala Phe Asp Leu Asp

420 425 430

ggt aga att gaa gtt gaa acc cca aac gaa gct acc att gtt att gaa 1344

Gly Arg Ile Glu Val Glu Thr Pro Asn Glu Ala Thr Ile Val Ile Glu

435 440 445

aga caa aac aaa gat ggt tcc cac att gcc aag gct gaa atc aac ttg 1392

Arg Gln Asn Lys Asp Gly Ser His Ile Ala Lys Ala Glu Ile Asn Leu

450 455 460

caa gac atg act tac cgt gtc act gaa aac gac caa acc att tct ttc 1440

Gln Asp Met Thr Tyr Arg Val Thr Glu Asn Asp Gln Thr Ile Ser Phe

465 470 475 480

gaa 1443

Glu

<210> 48

<211> 481

<212> PRT

<213> Streptococcus mutans

<400> 48

Met Pro Ile Ile Asn Lys Thr Met Leu Ile Thr Tyr Ala Asp Ser Leu

1 5 10 15

Gly Lys Asn Leu Lys Glu Leu Asn Glu Asn Ile Glu Asn Tyr Phe Gly

20 25 30

Asp Ala Val Gly Gly Val His Leu Leu Pro Phe Phe Pro Ser Thr Gly

35 40 45

Asp Arg Gly Phe Ala Pro Ile Asp Tyr His Glu Val Asp Ser Ala Phe

50 55 60

Gly Asp Trp Asp Asp Val Lys Cys Leu Gly Glu Lys Tyr Tyr Leu Met

65 70 75 80

Phe Asp Phe Met Ile Asn His Ile Ser Arg Gln Ser Lys Tyr Tyr Lys

85 90 95

Asp Tyr Gln Glu Lys His Glu Ala Ser Ala Tyr Lys Asp Leu Phe Leu

100 105 110

Asn Trp Asp Lys Phe Trp Pro Lys Asn Arg Pro Thr Gln Glu Asp Val

115 120 125

Asp Leu Ile Tyr Lys Arg Lys Asp Arg Ala Pro Lys Gln Glu Ile Gln

130 135 140

Phe Ala Asp Gly Ser Val Glu His Leu Trp Asn Thr Phe Gly Glu Glu

145 150 155 160

Gln Ile Asp Leu Asp Val Thr Lys Glu Val Thr Met Asp Phe Ile Arg

165 170 175

Ser Thr Ile Glu Asn Leu Ala Ala Asn Gly Cys Asp Leu Ile Arg Leu

180 185 190

Asp Ala Phe Ala Tyr Ala Val Lys Lys Leu Asp Thr Asn Asp Phe Phe

195 200 205

Val Glu Pro Glu Ile Trp Thr Leu Leu Asp Lys Val Arg Asp Ile Ala

210 215 220

Ala Val Ser Gly Ala Glu Ile Leu Pro Glu Ile His Glu His Tyr Thr

225 230 235 240

Ile Gln Phe Lys Ile Ala Asp His Asp Tyr Tyr Val Tyr Asp Phe Ala

245 250 255

Leu Pro Met Val Thr Leu Tyr Ser Leu Tyr Ser Ser Lys Val Asp Arg

260 265 270

Leu Ala Lys Trp Leu Lys Met Ser Pro Met Lys Gln Phe Thr Thr Leu

275 280 285

Asp Thr His Asp Gly Ile Gly Val Val Asp Val Lys Asp Ile Leu Thr

290 295 300

Asp Glu Glu Ile Thr Tyr Thr Ser Asn Glu Leu Tyr Lys Val Gly Ala

305 310 315 320

Asn Val Asn Arg Lys Tyr Ser Thr Ala Glu Tyr Asn Asn Leu Asp Ile

325 330 335

Tyr Gln Ile Asn Ser Thr Tyr Tyr Ser Ala Leu Gly Asp Asp Asp Gln

340 345 350

Lys Tyr Phe Leu Ala Arg Leu Ile Gln Ala Phe Ala Pro Gly Ile Pro

355 360 365

Gln Val Tyr Tyr Val Gly Phe Leu Ala Gly Lys Asn Asp Leu Glu Leu

370 375 380

Leu Glu Ser Thr Lys Glu Gly Arg Asn Ile Asn Arg His Tyr Tyr Ser

385 390 395 400

Ser Glu Glu Ile Ala Lys Glu Val Lys Arg Pro Val Val Lys Ala Leu

405 410 415

Leu Asn Leu Phe Thr Tyr Arg Asn Gln Ser Ala Ala Phe Asp Leu Asp

420 425 430

Gly Arg Ile Glu Val Glu Thr Pro Asn Glu Ala Thr Ile Val Ile Glu

435 440 445

Arg Gln Asn Lys Asp Gly Ser His Ile Ala Lys Ala Glu Ile Asn Leu

450 455 460

Gln Asp Met Thr Tyr Arg Val Thr Glu Asn Asp Gln Thr Ile Ser Phe

465 470 475 480

Glu

<210> 49

<211> 1392

<212> DNA

<213> Lobelia erinus

<220>

<221> CDS

<222> (1)..(1392)

<400> 49

atg gac aac aac cat ttg ggt gaa act ttg ttg cca ttg gct cca aag 48

Met Asp Asn Asn His Leu Gly Glu Thr Leu Leu Pro Leu Ala Pro Lys

1 5 10 15

aac ggt aga aga gtc ttg ttc ttc cca tac cca tta caa ggt cac att 96

Asn Gly Arg Arg Val Leu Phe Phe Pro Tyr Pro Leu Gln Gly His Ile

20 25 30

tct cca atg ttg aac ttg gcc aac ttg ttg cac tcc aag ggt ttc acc 144

Ser Pro Met Leu Asn Leu Ala Asn Leu Leu His Ser Lys Gly Phe Thr

35 40 45

atc acc atc atc cac acc aat ttg aac tct cca aac caa tct gac tac 192

Ile Thr Ile Ile His Thr Asn Leu Asn Ser Pro Asn Gln Ser Asp Tyr

50 55 60

cca cac ttc act ttc aga cca ttt gat gac ggt ttc cca cca tac tcc 240

Pro His Phe Thr Phe Arg Pro Phe Asp Asp Gly Phe Pro Pro Tyr Ser

65 70 75 80

aag ggt tgg caa ttg gct acc ttg tgt tcc aga tgt gtt gaa cca ttc 288

Lys Gly Trp Gln Leu Ala Thr Leu Cys Ser Arg Cys Val Glu Pro Phe

85 90 95

aga gaa tgt ttg gct caa atc ttc ttg tct gac cac act gct cca gaa 336

Arg Glu Cys Leu Ala Gln Ile Phe Leu Ser Asp His Thr Ala Pro Glu

100 105 110

ggt gaa aga gaa tct att gct tgt ttg att gct gat ggt tta tgg aac 384

Gly Glu Arg Glu Ser Ile Ala Cys Leu Ile Ala Asp Gly Leu Trp Asn

115 120 125

ttc ttg ggt gct gct gtc tac aac ttt aaa ttg cca atg att gtt ttg 432

Phe Leu Gly Ala Ala Val Tyr Asn Phe Lys Leu Pro Met Ile Val Leu

130 135 140

aga act ggt aac atg tct aac att gtt gcc aac gtt aag ttg cca tgt 480

Arg Thr Gly Asn Met Ser Asn Ile Val Ala Asn Val Lys Leu Pro Cys

145 150 155 160

ttc atc gaa aag ggt tac ttc gac cat acc aag gaa ggt tcc aag ttg 528

Phe Ile Glu Lys Gly Tyr Phe Asp His Thr Lys Glu Gly Ser Lys Leu

165 170 175

gaa gct gct gtc cca gaa ttc cca acc atc aag ttc aaa gat atc ttg 576

Glu Ala Ala Val Pro Glu Phe Pro Thr Ile Lys Phe Lys Asp Ile Leu

180 185 190

aaa acc tac ggt tct aac cca aag gcc atc tgt gaa act ttg act gct 624

Lys Thr Tyr Gly Ser Asn Pro Lys Ala Ile Cys Glu Thr Leu Thr Ala

195 200 205

ttg ttg aag gaa atg aga gct tct tct ggt gtc atc tgg aac tct tgt 672

Leu Leu Lys Glu Met Arg Ala Ser Ser Gly Val Ile Trp Asn Ser Cys

210 215 220

aag gaa tta gaa caa tct gaa tta caa atg atc tgt aag gaa ttc cca 720

Lys Glu Leu Glu Gln Ser Glu Leu Gln Met Ile Cys Lys Glu Phe Pro

225 230 235 240

gtt cct cat ttc ttg att ggt cct ttg cac aaa tac ttc cca gct tct 768

Val Pro His Phe Leu Ile Gly Pro Leu His Lys Tyr Phe Pro Ala Ser

245 250 255

tct tct tcc ttg gtt gcc cac gac cca tct tcc att tcc tgg ttg aac 816

Ser Ser Ser Leu Val Ala His Asp Pro Ser Ser Ile Ser Trp Leu Asn

260 265 270

tcc aag gct cca aac tct gtt ttg tac gtt tct ttc ggt tcc atc tct 864

Ser Lys Ala Pro Asn Ser Val Leu Tyr Val Ser Phe Gly Ser Ile Ser

275 280 285

tcc atg gac gaa gct gaa ttt cta gaa act gct tgg ggt ttg gcc aac 912

Ser Met Asp Glu Ala Glu Phe Leu Glu Thr Ala Trp Gly Leu Ala Asn

290 295 300

tcc atg caa caa ttc tta tgg gtt gtc aga cca ggt tct gtc aga ggt 960

Ser Met Gln Gln Phe Leu Trp Val Val Arg Pro Gly Ser Val Arg Gly

305 310 315 320

tct caa tgg ttg gaa tct tta cca gat ggt ttc att gac aag ttg gat 1008

Ser Gln Trp Leu Glu Ser Leu Pro Asp Gly Phe Ile Asp Lys Leu Asp

325 330 335

ggt aga ggt cac att gtc aaa tgg gct cct caa caa gaa gtc tta gct 1056

Gly Arg Gly His Ile Val Lys Trp Ala Pro Gln Gln Glu Val Leu Ala

340 345 350

cac caa gct acc ggt ggt ttc tgg act cac tgt ggt tgg aac tcc act 1104

His Gln Ala Thr Gly Gly Phe Trp Thr His Cys Gly Trp Asn Ser Thr

355 360 365

tta gaa tcc atg tgt gaa ggt gtc cca atg att tgt tcc cac ggt atc 1152

Leu Glu Ser Met Cys Glu Gly Val Pro Met Ile Cys Ser His Gly Ile

370 375 380

atg gac caa cca atc aat gct cgt tac gtt acc gat gtc tgg aag gtt 1200

Met Asp Gln Pro Ile Asn Ala Arg Tyr Val Thr Asp Val Trp Lys Val

385 390 395 400

ggt att gaa ttg gaa aag ggt ttt gac tct gaa gaa atc aag atg gcc 1248

Gly Ile Glu Leu Glu Lys Gly Phe Asp Ser Glu Glu Ile Lys Met Ala

405 410 415

atc cgt cgt ttg atg gtt gac aag gaa ggt caa gaa atc aga gaa aga 1296

Ile Arg Arg Leu Met Val Asp Lys Glu Gly Gln Glu Ile Arg Glu Arg

420 425 430

tct tcc aga ttg aag gaa tct ttg tcc aac tgt ttg aag caa ggt ggt 1344

Ser Ser Arg Leu Lys Glu Ser Leu Ser Asn Cys Leu Lys Gln Gly Gly

435 440 445

tct tcc cac gat tct gtc gaa tct ttg gtt gac cac atc cta tcc ttc 1392

Ser Ser His Asp Ser Val Glu Ser Leu Val Asp His Ile Leu Ser Phe

450 455 460

<210> 50

<211> 464

<212> PRT

<213> Lobelia erinus

<400> 50

Met Asp Asn Asn His Leu Gly Glu Thr Leu Leu Pro Leu Ala Pro Lys

1 5 10 15

Asn Gly Arg Arg Val Leu Phe Phe Pro Tyr Pro Leu Gln Gly His Ile

20 25 30

Ser Pro Met Leu Asn Leu Ala Asn Leu Leu His Ser Lys Gly Phe Thr

35 40 45

Ile Thr Ile Ile His Thr Asn Leu Asn Ser Pro Asn Gln Ser Asp Tyr

50 55 60

Pro His Phe Thr Phe Arg Pro Phe Asp Asp Gly Phe Pro Pro Tyr Ser

65 70 75 80

Lys Gly Trp Gln Leu Ala Thr Leu Cys Ser Arg Cys Val Glu Pro Phe

85 90 95

Arg Glu Cys Leu Ala Gln Ile Phe Leu Ser Asp His Thr Ala Pro Glu

100 105 110

Gly Glu Arg Glu Ser Ile Ala Cys Leu Ile Ala Asp Gly Leu Trp Asn

115 120 125

Phe Leu Gly Ala Ala Val Tyr Asn Phe Lys Leu Pro Met Ile Val Leu

130 135 140

Arg Thr Gly Asn Met Ser Asn Ile Val Ala Asn Val Lys Leu Pro Cys

145 150 155 160

Phe Ile Glu Lys Gly Tyr Phe Asp His Thr Lys Glu Gly Ser Lys Leu

165 170 175

Glu Ala Ala Val Pro Glu Phe Pro Thr Ile Lys Phe Lys Asp Ile Leu

180 185 190

Lys Thr Tyr Gly Ser Asn Pro Lys Ala Ile Cys Glu Thr Leu Thr Ala

195 200 205

Leu Leu Lys Glu Met Arg Ala Ser Ser Gly Val Ile Trp Asn Ser Cys

210 215 220

Lys Glu Leu Glu Gln Ser Glu Leu Gln Met Ile Cys Lys Glu Phe Pro

225 230 235 240

Val Pro His Phe Leu Ile Gly Pro Leu His Lys Tyr Phe Pro Ala Ser

245 250 255

Ser Ser Ser Leu Val Ala His Asp Pro Ser Ser Ile Ser Trp Leu Asn

260 265 270

Ser Lys Ala Pro Asn Ser Val Leu Tyr Val Ser Phe Gly Ser Ile Ser

275 280 285

Ser Met Asp Glu Ala Glu Phe Leu Glu Thr Ala Trp Gly Leu Ala Asn

290 295 300

Ser Met Gln Gln Phe Leu Trp Val Val Arg Pro Gly Ser Val Arg Gly

305 310 315 320

Ser Gln Trp Leu Glu Ser Leu Pro Asp Gly Phe Ile Asp Lys Leu Asp

325 330 335

Gly Arg Gly His Ile Val Lys Trp Ala Pro Gln Gln Glu Val Leu Ala

340 345 350

His Gln Ala Thr Gly Gly Phe Trp Thr His Cys Gly Trp Asn Ser Thr

355 360 365

Leu Glu Ser Met Cys Glu Gly Val Pro Met Ile Cys Ser His Gly Ile

370 375 380

Met Asp Gln Pro Ile Asn Ala Arg Tyr Val Thr Asp Val Trp Lys Val

385 390 395 400

Gly Ile Glu Leu Glu Lys Gly Phe Asp Ser Glu Glu Ile Lys Met Ala

405 410 415

Ile Arg Arg Leu Met Val Asp Lys Glu Gly Gln Glu Ile Arg Glu Arg

420 425 430

Ser Ser Arg Leu Lys Glu Ser Leu Ser Asn Cys Leu Lys Gln Gly Gly

435 440 445

Ser Ser His Asp Ser Val Glu Ser Leu Val Asp His Ile Leu Ser Phe

450 455 460

<210> 51

<211> 1380

<212> DNA

<213> Arabidopsis thaliana

<220>

<221> CDS

<222> (1)..(1380)

<400> 51

atg gaa gaa aga aag ggt aga aga atc atc atg ttc cca tta cca ttc 48

Met Glu Glu Arg Lys Gly Arg Arg Ile Ile Met Phe Pro Leu Pro Phe

1 5 10 15

cca ggt cac ttc aac cca atg att gaa ttg gct ggt atc ttt cat cac 96

Pro Gly His Phe Asn Pro Met Ile Glu Leu Ala Gly Ile Phe His His

20 25 30

cgt ggt ttc tcc gtt acc atc ttg cac act tct tac aac ttc cca gac 144

Arg Gly Phe Ser Val Thr Ile Leu His Thr Ser Tyr Asn Phe Pro Asp

35 40 45

cca tcc aga cac cca cac ttc act ttc aga acc att tct cac aac aag 192

Pro Ser Arg His Pro His Phe Thr Phe Arg Thr Ile Ser His Asn Lys

50 55 60

gaa ggt gaa gaa gat cca tta tct caa tct gaa acc tct tcc atg gac 240

Glu Gly Glu Glu Asp Pro Leu Ser Gln Ser Glu Thr Ser Ser Met Asp

65 70 75 80

ttg att gtc ttg gtc aga aga tta aag caa cgt tac gct gaa cca ttc 288

Leu Ile Val Leu Val Arg Arg Leu Lys Gln Arg Tyr Ala Glu Pro Phe

85 90 95

aga aag tcc gtt gct gct gaa gtc ggt ggt ggt gaa acc gtt tgt tgt 336

Arg Lys Ser Val Ala Ala Glu Val Gly Gly Gly Glu Thr Val Cys Cys

100 105 110

ttg gtt tct gat gct atc tgg ggt aag aac act gaa gtt gtt gct gaa 384

Leu Val Ser Asp Ala Ile Trp Gly Lys Asn Thr Glu Val Val Ala Glu

115 120 125

gaa atc ggt gtc aga aga gtt gtt ttg aga act ggt ggt gcc tct tct 432

Glu Ile Gly Val Arg Arg Val Val Leu Arg Thr Gly Gly Ala Ser Ser

130 135 140

ttc tgt gcc ttt gct gct ttc cca tta ttg aga gac aag ggt tac ttg 480

Phe Cys Ala Phe Ala Ala Phe Pro Leu Leu Arg Asp Lys Gly Tyr Leu

145 150 155 160

cca atc caa gat tct cgt ttg gat gaa cct gtt act gaa ttg cct cca 528

Pro Ile Gln Asp Ser Arg Leu Asp Glu Pro Val Thr Glu Leu Pro Pro

165 170 175

ttg aag gtc aag gac tta cca gtc atg gaa acc aat gaa cca gaa gaa 576

Leu Lys Val Lys Asp Leu Pro Val Met Glu Thr Asn Glu Pro Glu Glu

180 185 190

ttg tac aga gtt gtt aac gac atg gtt gaa ggt gct aaa tct tct tct 624

Leu Tyr Arg Val Val Asn Asp Met Val Glu Gly Ala Lys Ser Ser Ser

195 200 205

ggt gtc atc tgg aac act ttc gaa gat ttg gaa aga ttg tct ttg atg 672

Gly Val Ile Trp Asn Thr Phe Glu Asp Leu Glu Arg Leu Ser Leu Met

210 215 220

aac tgt tcc tcc aaa ttg caa gtt cca ttc ttc cca atc ggt cca ttc 720

Asn Cys Ser Ser Lys Leu Gln Val Pro Phe Phe Pro Ile Gly Pro Phe

225 230 235 240

cac aag tac tct gaa gat cca act cca aag act gaa aac aag gaa gat 768

His Lys Tyr Ser Glu Asp Pro Thr Pro Lys Thr Glu Asn Lys Glu Asp

245 250 255

acc gac tgg ttg gac aag caa gac cct caa tcc gtt gtc tac gcc tcc 816

Thr Asp Trp Leu Asp Lys Gln Asp Pro Gln Ser Val Val Tyr Ala Ser

260 265 270

ttt ggt tct ttg gcc gct att gaa gaa aag gaa ttc ttg gaa att gct 864

Phe Gly Ser Leu Ala Ala Ile Glu Glu Lys Glu Phe Leu Glu Ile Ala

275 280 285

tgg ggt cta aga aac tct gaa aga cca ttc tta tgg gtt gtt aga cca 912

Trp Gly Leu Arg Asn Ser Glu Arg Pro Phe Leu Trp Val Val Arg Pro

290 295 300

ggt tcc gtc cgt ggt act gaa tgg ttg gaa tct cta cca ttg ggt ttc 960

Gly Ser Val Arg Gly Thr Glu Trp Leu Glu Ser Leu Pro Leu Gly Phe

305 310 315 320

atg gaa aac atc ggt gac aag ggt aag att gtc aaa tgg gct aac caa 1008

Met Glu Asn Ile Gly Asp Lys Gly Lys Ile Val Lys Trp Ala Asn Gln

325 330 335

ttg gaa gtt ttg gct cac cca gcc att ggt gct ttc tgg acc cac tgt 1056

Leu Glu Val Leu Ala His Pro Ala Ile Gly Ala Phe Trp Thr His Cys

340 345 350

ggt tgg aac tcc act ttg gaa tcc atc tgt gaa ggt gtc cca atg atc 1104

Gly Trp Asn Ser Thr Leu Glu Ser Ile Cys Glu Gly Val Pro Met Ile

355 360 365

tgt acc tcc tgt ttt acc gac caa cat gtc aac gcc aga tac att gtc 1152

Cys Thr Ser Cys Phe Thr Asp Gln His Val Asn Ala Arg Tyr Ile Val

370 375 380

gac gtc tgg aga gtc ggt atg ttg ttg gaa aga tcc aag atg gaa aag 1200

Asp Val Trp Arg Val Gly Met Leu Leu Glu Arg Ser Lys Met Glu Lys

385 390 395 400

aag gaa atc gaa aag gtt ttg aga tct gtt atg atg gaa aag ggt gat 1248

Lys Glu Ile Glu Lys Val Leu Arg Ser Val Met Met Glu Lys Gly Asp

405 410 415

ggt ttg aga gaa aga tct ttg aaa ttg aag gaa aga gct gat ttc tgt 1296

Gly Leu Arg Glu Arg Ser Leu Lys Leu Lys Glu Arg Ala Asp Phe Cys

420 425 430

ttg tcc aag gac ggt tct tct tcc aag tac ttg gac aaa ttg gtt tcc 1344

Leu Ser Lys Asp Gly Ser Ser Ser Lys Tyr Leu Asp Lys Leu Val Ser

435 440 445

cac gtc tta tct ttc gac tct tac gct ttc gct tct 1380

His Val Leu Ser Phe Asp Ser Tyr Ala Phe Ala Ser

450 455 460

<210> 52

<211> 460

<212> PRT

<213> Arabidopsis thaliana

<400> 52

Met Glu Glu Arg Lys Gly Arg Arg Ile Ile Met Phe Pro Leu Pro Phe

1 5 10 15

Pro Gly His Phe Asn Pro Met Ile Glu Leu Ala Gly Ile Phe His His

20 25 30

Arg Gly Phe Ser Val Thr Ile Leu His Thr Ser Tyr Asn Phe Pro Asp

35 40 45

Pro Ser Arg His Pro His Phe Thr Phe Arg Thr Ile Ser His Asn Lys

50 55 60

Glu Gly Glu Glu Asp Pro Leu Ser Gln Ser Glu Thr Ser Ser Met Asp

65 70 75 80

Leu Ile Val Leu Val Arg Arg Leu Lys Gln Arg Tyr Ala Glu Pro Phe

85 90 95

Arg Lys Ser Val Ala Ala Glu Val Gly Gly Gly Glu Thr Val Cys Cys

100 105 110

Leu Val Ser Asp Ala Ile Trp Gly Lys Asn Thr Glu Val Val Ala Glu

115 120 125

Glu Ile Gly Val Arg Arg Val Val Leu Arg Thr Gly Gly Ala Ser Ser

130 135 140

Phe Cys Ala Phe Ala Ala Phe Pro Leu Leu Arg Asp Lys Gly Tyr Leu

145 150 155 160

Pro Ile Gln Asp Ser Arg Leu Asp Glu Pro Val Thr Glu Leu Pro Pro

165 170 175

Leu Lys Val Lys Asp Leu Pro Val Met Glu Thr Asn Glu Pro Glu Glu

180 185 190

Leu Tyr Arg Val Val Asn Asp Met Val Glu Gly Ala Lys Ser Ser Ser

195 200 205

Gly Val Ile Trp Asn Thr Phe Glu Asp Leu Glu Arg Leu Ser Leu Met

210 215 220

Asn Cys Ser Ser Lys Leu Gln Val Pro Phe Phe Pro Ile Gly Pro Phe

225 230 235 240

His Lys Tyr Ser Glu Asp Pro Thr Pro Lys Thr Glu Asn Lys Glu Asp

245 250 255

Thr Asp Trp Leu Asp Lys Gln Asp Pro Gln Ser Val Val Tyr Ala Ser

260 265 270

Phe Gly Ser Leu Ala Ala Ile Glu Glu Lys Glu Phe Leu Glu Ile Ala

275 280 285

Trp Gly Leu Arg Asn Ser Glu Arg Pro Phe Leu Trp Val Val Arg Pro

290 295 300

Gly Ser Val Arg Gly Thr Glu Trp Leu Glu Ser Leu Pro Leu Gly Phe

305 310 315 320

Met Glu Asn Ile Gly Asp Lys Gly Lys Ile Val Lys Trp Ala Asn Gln

325 330 335

Leu Glu Val Leu Ala His Pro Ala Ile Gly Ala Phe Trp Thr His Cys

340 345 350

Gly Trp Asn Ser Thr Leu Glu Ser Ile Cys Glu Gly Val Pro Met Ile

355 360 365

Cys Thr Ser Cys Phe Thr Asp Gln His Val Asn Ala Arg Tyr Ile Val

370 375 380

Asp Val Trp Arg Val Gly Met Leu Leu Glu Arg Ser Lys Met Glu Lys

385 390 395 400

Lys Glu Ile Glu Lys Val Leu Arg Ser Val Met Met Glu Lys Gly Asp

405 410 415

Gly Leu Arg Glu Arg Ser Leu Lys Leu Lys Glu Arg Ala Asp Phe Cys

420 425 430

Leu Ser Lys Asp Gly Ser Ser Ser Lys Tyr Leu Asp Lys Leu Val Ser

435 440 445

His Val Leu Ser Phe Asp Ser Tyr Ala Phe Ala Ser

450 455 460

<210> 53

<211> 2139

<212> DNA

<213> Gibberella fujikuroi

<220>

<221> CDS

<222> (1)..(2139)

<400> 53

atg gct gaa ttg gac act ttg gat atc gtc gtt ttg ggt gtt atc ttc 48

Met Ala Glu Leu Asp Thr Leu Asp Ile Val Val Leu Gly Val Ile Phe

1 5 10 15

ttg ggt act gtt gct tac ttc acc aag ggt aag ttg tgg ggt gtc acc 96

Leu Gly Thr Val Ala Tyr Phe Thr Lys Gly Lys Leu Trp Gly Val Thr

20 25 30

aag gat cca tac gct aac ggt ttt gcc gct ggt ggt gct tcc aag cca 144

Lys Asp Pro Tyr Ala Asn Gly Phe Ala Ala Gly Gly Ala Ser Lys Pro

35 40 45

ggt aga acc aga aac att gtt gaa gcc atg gaa gaa tct ggt aag aac 192

Gly Arg Thr Arg Asn Ile Val Glu Ala Met Glu Glu Ser Gly Lys Asn

50 55 60

tgt gtt gtt ttc tac ggt tct caa acc ggt act gct gaa gat tac gct 240

Cys Val Val Phe Tyr Gly Ser Gln Thr Gly Thr Ala Glu Asp Tyr Ala

65 70 75 80

tcc aga tta gct aag gaa ggt aag tcc aga ttc ggt ttg aac acc atg 288

Ser Arg Leu Ala Lys Glu Gly Lys Ser Arg Phe Gly Leu Asn Thr Met

85 90 95

att gct gac tta gaa gat tac gac ttc gac aac ttg gat acc gtt cca 336

Ile Ala Asp Leu Glu Asp Tyr Asp Phe Asp Asn Leu Asp Thr Val Pro

100 105 110

tct gac aac att gtc atg ttc gtt ttg gct acc tac ggt gaa ggt gaa 384

Ser Asp Asn Ile Val Met Phe Val Leu Ala Thr Tyr Gly Glu Gly Glu

115 120 125

cct acc gac aat gct gtt gac ttc tac gaa ttc atc act ggt gaa gat 432

Pro Thr Asp Asn Ala Val Asp Phe Tyr Glu Phe Ile Thr Gly Glu Asp

130 135 140

gct tct ttc aac gaa ggt aac gac cct cca ttg ggt aac ttg aat tat 480

Ala Ser Phe Asn Glu Gly Asn Asp Pro Pro Leu Gly Asn Leu Asn Tyr

145 150 155 160

gtc gct ttc ggt tta ggt aac aac acc tac gaa cac tac aac tcc atg 528

Val Ala Phe Gly Leu Gly Asn Asn Thr Tyr Glu His Tyr Asn Ser Met

165 170 175

gtt aga aat gtc aac aaa gct ttg gaa aag cta ggt gcc cac aga att 576

Val Arg Asn Val Asn Lys Ala Leu Glu Lys Leu Gly Ala His Arg Ile

180 185 190

ggt gaa gct ggt gaa ggt gac gac ggt gct ggt act atg gaa gaa gat 624

Gly Glu Ala Gly Glu Gly Asp Asp Gly Ala Gly Thr Met Glu Glu Asp

195 200 205

ttc ttg gct tgg aag gac cca atg tgg gaa gct ttg gct aag aag atg 672

Phe Leu Ala Trp Lys Asp Pro Met Trp Glu Ala Leu Ala Lys Lys Met

210 215 220

ggt ttg gaa gaa aga gaa gct gtt tac gaa cca atc ttt gct atc aac 720

Gly Leu Glu Glu Arg Glu Ala Val Tyr Glu Pro Ile Phe Ala Ile Asn

225 230 235 240

gaa aga gat gac ttg act cca gaa gct aac gaa gtt tac ttg ggt gaa 768

Glu Arg Asp Asp Leu Thr Pro Glu Ala Asn Glu Val Tyr Leu Gly Glu

245 250 255

cca aat aaa ttg cac ttg gaa ggt act gct aag ggt cct ttc aac tct 816

Pro Asn Lys Leu His Leu Glu Gly Thr Ala Lys Gly Pro Phe Asn Ser

260 265 270

cac aac cca tac att gct cca att gct gaa tct tac gaa ttg ttc tct 864

His Asn Pro Tyr Ile Ala Pro Ile Ala Glu Ser Tyr Glu Leu Phe Ser

275 280 285

gcc aag gac aga aac tgt ttg cac atg gaa att gat atc tct ggt tcc 912

Ala Lys Asp Arg Asn Cys Leu His Met Glu Ile Asp Ile Ser Gly Ser

290 295 300

aac ttg aaa tac gaa act ggt gac cac att gcc atc tgg cca acc aac 960

Asn Leu Lys Tyr Glu Thr Gly Asp His Ile Ala Ile Trp Pro Thr Asn

305 310 315 320

cca ggt gaa gaa gtc aac aaa ttc ttg gat atc ttg gac tta tcc ggt 1008

Pro Gly Glu Glu Val Asn Lys Phe Leu Asp Ile Leu Asp Leu Ser Gly

325 330 335

aag caa cac tct gtt gtt acc gtt aag gcc ttg gaa cca act gcc aag 1056

Lys Gln His Ser Val Val Thr Val Lys Ala Leu Glu Pro Thr Ala Lys

340 345 350

gtc cca ttc cca aac cca acc act tac gat gct atc cta aga tac cac 1104

Val Pro Phe Pro Asn Pro Thr Thr Tyr Asp Ala Ile Leu Arg Tyr His

355 360 365

ttg gaa atc tgt gcc cca gtt tcc cgt caa ttt gtc tct act ttg gct 1152

Leu Glu Ile Cys Ala Pro Val Ser Arg Gln Phe Val Ser Thr Leu Ala

370 375 380

gcc ttt gct cca aac gac gac atc aag gct gaa atg aac aga ttg ggt 1200

Ala Phe Ala Pro Asn Asp Asp Ile Lys Ala Glu Met Asn Arg Leu Gly

385 390 395 400

tct gac aag gac tac ttc cat gaa aag act ggt cca cac tac tac aac 1248

Ser Asp Lys Asp Tyr Phe His Glu Lys Thr Gly Pro His Tyr Tyr Asn

405 410 415

att gcc aga ttc tta gct tcc gtt tct aag ggt gaa aag tgg acc aag 1296

Ile Ala Arg Phe Leu Ala Ser Val Ser Lys Gly Glu Lys Trp Thr Lys

420 425 430

att cca ttt tcc gct ttc atc gaa ggt ttg acc aaa tta caa cca aga 1344

Ile Pro Phe Ser Ala Phe Ile Glu Gly Leu Thr Lys Leu Gln Pro Arg

435 440 445

tac tac tcc atc tcc tct tct tct ttg gtc caa cca aag aag atc tcc 1392

Tyr Tyr Ser Ile Ser Ser Ser Ser Leu Val Gln Pro Lys Lys Ile Ser

450 455 460

atc act gcc gtc gtt gaa tct caa caa att cca ggt aga gat gac cca 1440

Ile Thr Ala Val Val Glu Ser Gln Gln Ile Pro Gly Arg Asp Asp Pro

465 470 475 480

ttc aga ggt gtc gcc acc aac tac ttg ttc gct ttg aag caa aag caa 1488

Phe Arg Gly Val Ala Thr Asn Tyr Leu Phe Ala Leu Lys Gln Lys Gln

485 490 495

aac ggt gac cca aac cca gct cca ttc ggt caa tct tac gaa ttg acc 1536

Asn Gly Asp Pro Asn Pro Ala Pro Phe Gly Gln Ser Tyr Glu Leu Thr

500 505 510

ggt cca aga aac aaa tac gat ggt att cat gtt cca gtc cac gtc aga 1584

Gly Pro Arg Asn Lys Tyr Asp Gly Ile His Val Pro Val His Val Arg

515 520 525

cac tcc aac ttt aaa ttg cct tct gac cca ggt aag cca atc atc atg 1632

His Ser Asn Phe Lys Leu Pro Ser Asp Pro Gly Lys Pro Ile Ile Met

530 535 540

atc ggt cca ggt act ggt gtt gct cca ttc aga ggt ttc gtt caa gaa 1680

Ile Gly Pro Gly Thr Gly Val Ala Pro Phe Arg Gly Phe Val Gln Glu

545 550 555 560

aga gcc aag caa gct aga gat ggt gtc gaa gtc ggt aag act ttg tta 1728

Arg Ala Lys Gln Ala Arg Asp Gly Val Glu Val Gly Lys Thr Leu Leu

565 570 575

ttc ttc ggt tgt cgt aaa tct act gaa gat ttc atg tac caa aag gaa 1776

Phe Phe Gly Cys Arg Lys Ser Thr Glu Asp Phe Met Tyr Gln Lys Glu

580 585 590

tgg caa gaa tac aaa gaa gct ttg ggt gac aag ttc gaa atg atc act 1824

Trp Gln Glu Tyr Lys Glu Ala Leu Gly Asp Lys Phe Glu Met Ile Thr

595 600 605

gct ttc tcc aga gaa ggt tcc aag aag gtt tac gtc caa cat cgt ttg 1872

Ala Phe Ser Arg Glu Gly Ser Lys Lys Val Tyr Val Gln His Arg Leu

610 615 620

aag gaa aga tcc aag gaa gtt tcc gac ttg ttg tct caa aag gcc tac 1920

Lys Glu Arg Ser Lys Glu Val Ser Asp Leu Leu Ser Gln Lys Ala Tyr

625 630 635 640

ttc tac gtt tgt ggt gac gct gct cac atg gct cgt gaa gtc aac acc 1968

Phe Tyr Val Cys Gly Asp Ala Ala His Met Ala Arg Glu Val Asn Thr

645 650 655

gtc ttg gct caa atc att gct gaa ggt aga ggt gtc tct gaa gct aag 2016

Val Leu Ala Gln Ile Ile Ala Glu Gly Arg Gly Val Ser Glu Ala Lys

660 665 670

ggt gaa gaa att gtc aag aac atg aga tct gcc aac caa tac caa gtt 2064

Gly Glu Glu Ile Val Lys Asn Met Arg Ser Ala Asn Gln Tyr Gln Val

675 680 685

tgt tct gat ttc gtt act tta cac tgt aag gaa acc acc tac gct aac 2112

Cys Ser Asp Phe Val Thr Leu His Cys Lys Glu Thr Thr Tyr Ala Asn

690 695 700

tct gaa ttg caa gaa gat gtc tgg tct 2139

Ser Glu Leu Gln Glu Asp Val Trp Ser

705 710

<210> 54

<211> 713

<212> PRT

<213> Gibberella fujikuroi

<400> 54

Met Ala Glu Leu Asp Thr Leu Asp Ile Val Val Leu Gly Val Ile Phe

1 5 10 15

Leu Gly Thr Val Ala Tyr Phe Thr Lys Gly Lys Leu Trp Gly Val Thr

20 25 30

Lys Asp Pro Tyr Ala Asn Gly Phe Ala Ala Gly Gly Ala Ser Lys Pro

35 40 45

Gly Arg Thr Arg Asn Ile Val Glu Ala Met Glu Glu Ser Gly Lys Asn

50 55 60

Cys Val Val Phe Tyr Gly Ser Gln Thr Gly Thr Ala Glu Asp Tyr Ala

65 70 75 80

Ser Arg Leu Ala Lys Glu Gly Lys Ser Arg Phe Gly Leu Asn Thr Met

85 90 95

Ile Ala Asp Leu Glu Asp Tyr Asp Phe Asp Asn Leu Asp Thr Val Pro

100 105 110

Ser Asp Asn Ile Val Met Phe Val Leu Ala Thr Tyr Gly Glu Gly Glu

115 120 125

Pro Thr Asp Asn Ala Val Asp Phe Tyr Glu Phe Ile Thr Gly Glu Asp

130 135 140

Ala Ser Phe Asn Glu Gly Asn Asp Pro Pro Leu Gly Asn Leu Asn Tyr

145 150 155 160

Val Ala Phe Gly Leu Gly Asn Asn Thr Tyr Glu His Tyr Asn Ser Met

165 170 175

Val Arg Asn Val Asn Lys Ala Leu Glu Lys Leu Gly Ala His Arg Ile

180 185 190

Gly Glu Ala Gly Glu Gly Asp Asp Gly Ala Gly Thr Met Glu Glu Asp

195 200 205

Phe Leu Ala Trp Lys Asp Pro Met Trp Glu Ala Leu Ala Lys Lys Met

210 215 220

Gly Leu Glu Glu Arg Glu Ala Val Tyr Glu Pro Ile Phe Ala Ile Asn

225 230 235 240

Glu Arg Asp Asp Leu Thr Pro Glu Ala Asn Glu Val Tyr Leu Gly Glu

245 250 255

Pro Asn Lys Leu His Leu Glu Gly Thr Ala Lys Gly Pro Phe Asn Ser

260 265 270

His Asn Pro Tyr Ile Ala Pro Ile Ala Glu Ser Tyr Glu Leu Phe Ser

275 280 285

Ala Lys Asp Arg Asn Cys Leu His Met Glu Ile Asp Ile Ser Gly Ser

290 295 300

Asn Leu Lys Tyr Glu Thr Gly Asp His Ile Ala Ile Trp Pro Thr Asn

305 310 315 320

Pro Gly Glu Glu Val Asn Lys Phe Leu Asp Ile Leu Asp Leu Ser Gly

325 330 335

Lys Gln His Ser Val Val Thr Val Lys Ala Leu Glu Pro Thr Ala Lys

340 345 350

Val Pro Phe Pro Asn Pro Thr Thr Tyr Asp Ala Ile Leu Arg Tyr His

355 360 365

Leu Glu Ile Cys Ala Pro Val Ser Arg Gln Phe Val Ser Thr Leu Ala

370 375 380

Ala Phe Ala Pro Asn Asp Asp Ile Lys Ala Glu Met Asn Arg Leu Gly

385 390 395 400

Ser Asp Lys Asp Tyr Phe His Glu Lys Thr Gly Pro His Tyr Tyr Asn

405 410 415

Ile Ala Arg Phe Leu Ala Ser Val Ser Lys Gly Glu Lys Trp Thr Lys

420 425 430

Ile Pro Phe Ser Ala Phe Ile Glu Gly Leu Thr Lys Leu Gln Pro Arg

435 440 445

Tyr Tyr Ser Ile Ser Ser Ser Ser Leu Val Gln Pro Lys Lys Ile Ser

450 455 460

Ile Thr Ala Val Val Glu Ser Gln Gln Ile Pro Gly Arg Asp Asp Pro

465 470 475 480

Phe Arg Gly Val Ala Thr Asn Tyr Leu Phe Ala Leu Lys Gln Lys Gln

485 490 495

Asn Gly Asp Pro Asn Pro Ala Pro Phe Gly Gln Ser Tyr Glu Leu Thr

500 505 510

Gly Pro Arg Asn Lys Tyr Asp Gly Ile His Val Pro Val His Val Arg

515 520 525

His Ser Asn Phe Lys Leu Pro Ser Asp Pro Gly Lys Pro Ile Ile Met

530 535 540

Ile Gly Pro Gly Thr Gly Val Ala Pro Phe Arg Gly Phe Val Gln Glu

545 550 555 560

Arg Ala Lys Gln Ala Arg Asp Gly Val Glu Val Gly Lys Thr Leu Leu

565 570 575

Phe Phe Gly Cys Arg Lys Ser Thr Glu Asp Phe Met Tyr Gln Lys Glu

580 585 590

Trp Gln Glu Tyr Lys Glu Ala Leu Gly Asp Lys Phe Glu Met Ile Thr

595 600 605

Ala Phe Ser Arg Glu Gly Ser Lys Lys Val Tyr Val Gln His Arg Leu

610 615 620

Lys Glu Arg Ser Lys Glu Val Ser Asp Leu Leu Ser Gln Lys Ala Tyr

625 630 635 640

Phe Tyr Val Cys Gly Asp Ala Ala His Met Ala Arg Glu Val Asn Thr

645 650 655

Val Leu Ala Gln Ile Ile Ala Glu Gly Arg Gly Val Ser Glu Ala Lys

660 665 670

Gly Glu Glu Ile Val Lys Asn Met Arg Ser Ala Asn Gln Tyr Gln Val

675 680 685

Cys Ser Asp Phe Val Thr Leu His Cys Lys Glu Thr Thr Tyr Ala Asn

690 695 700

Ser Glu Leu Gln Glu Asp Val Trp Ser

705 710

<210> 55

<211> 2076

<212> DNA

<213> Arabidopsis thaliana

<220>

<221> CDS

<222> (1)..(2076)

<400> 55

atg acc tct gct tta tac gcc tcc gat ttg ttc aag caa ttg aag tcc 48

Met Thr Ser Ala Leu Tyr Ala Ser Asp Leu Phe Lys Gln Leu Lys Ser

1 5 10 15

atc atg ggt act gac tct ttg tcc gat gat gtt gtc tta gtc att gcc 96

Ile Met Gly Thr Asp Ser Leu Ser Asp Asp Val Val Leu Val Ile Ala

20 25 30

acc act tct ttg gct ttg gtt gct ggt ttc gtt gtc ttg ttg tgg aag 144

Thr Thr Ser Leu Ala Leu Val Ala Gly Phe Val Val Leu Leu Trp Lys

35 40 45

aaa acc acc gct gac aga tcc ggt gaa ttg aaa cct ttg atg att cca 192

Lys Thr Thr Ala Asp Arg Ser Gly Glu Leu Lys Pro Leu Met Ile Pro

50 55 60

aag tct ttg atg gcc aag gac gaa gat gac gac ttg gac ttg ggt tct 240

Lys Ser Leu Met Ala Lys Asp Glu Asp Asp Asp Leu Asp Leu Gly Ser

65 70 75 80

ggt aag acc aga gtc tcc att ttc ttc ggt act caa acc ggt act gct 288

Gly Lys Thr Arg Val Ser Ile Phe Phe Gly Thr Gln Thr Gly Thr Ala

85 90 95

gaa ggt ttc gct aag gct ttg tct gaa gaa att aaa gcc aga tac gaa 336

Glu Gly Phe Ala Lys Ala Leu Ser Glu Glu Ile Lys Ala Arg Tyr Glu

100 105 110

aag gct gcc gtt aag gtt atc gat ttg gat gac tac gct gct gac gat 384

Lys Ala Ala Val Lys Val Ile Asp Leu Asp Asp Tyr Ala Ala Asp Asp

115 120 125

gac caa tac gaa gaa aag ttg aag aag gaa act ttg gct ttc ttc tgt 432

Asp Gln Tyr Glu Glu Lys Leu Lys Lys Glu Thr Leu Ala Phe Phe Cys

130 135 140

gtt gct acc tac ggt gac ggt gaa cca act gac aat gct gct aga ttc 480

Val Ala Thr Tyr Gly Asp Gly Glu Pro Thr Asp Asn Ala Ala Arg Phe

145 150 155 160

tac aag tgg ttc acc gaa gaa aac gaa aga gac atc aaa ttg caa caa 528

Tyr Lys Trp Phe Thr Glu Glu Asn Glu Arg Asp Ile Lys Leu Gln Gln

165 170 175

ttg gct tac ggt gtc ttt gct ttg ggt aac aga caa tac gaa cat ttc 576

Leu Ala Tyr Gly Val Phe Ala Leu Gly Asn Arg Gln Tyr Glu His Phe

180 185 190

aac aag atc ggt att gtc ttg gac gaa gaa tta tgt aag aag ggt gcc 624

Asn Lys Ile Gly Ile Val Leu Asp Glu Glu Leu Cys Lys Lys Gly Ala

195 200 205

aag aga ttg att gaa gtc ggt ttg ggt gac gat gac caa tct att gaa 672

Lys Arg Leu Ile Glu Val Gly Leu Gly Asp Asp Asp Gln Ser Ile Glu

210 215 220

gat gac ttc aac gct tgg aag gaa tct ttg tgg tct gaa tta gac aag 720

Asp Asp Phe Asn Ala Trp Lys Glu Ser Leu Trp Ser Glu Leu Asp Lys

225 230 235 240

tta ttg aag gac gaa gat gac aag tcc gtt gct act cca tac act gct 768

Leu Leu Lys Asp Glu Asp Asp Lys Ser Val Ala Thr Pro Tyr Thr Ala

245 250 255

gtc atc cca gaa tac cgt gtc gtc acc cac gac cca aga ttc acc acc 816

Val Ile Pro Glu Tyr Arg Val Val Thr His Asp Pro Arg Phe Thr Thr

260 265 270

caa aag tcc atg gaa tcc aac gtt gcc aac ggt aac acc act att gac 864

Gln Lys Ser Met Glu Ser Asn Val Ala Asn Gly Asn Thr Thr Ile Asp

275 280 285

atc cac cac cca tgt cgt gtt gat gtt gct gtt caa aag gaa ttg cac 912

Ile His His Pro Cys Arg Val Asp Val Ala Val Gln Lys Glu Leu His

290 295 300

acc cac gaa tct gac cgt tct tgt att cac ttg gaa ttc gat atc tcc 960

Thr His Glu Ser Asp Arg Ser Cys Ile His Leu Glu Phe Asp Ile Ser

305 310 315 320

aga acc ggt atc act tac gaa act ggt gac cac gtc ggt gtc tac gct 1008

Arg Thr Gly Ile Thr Tyr Glu Thr Gly Asp His Val Gly Val Tyr Ala

325 330 335

gaa aac cac gtt gaa atc gtc gaa gaa gcc ggt aaa ttg ttg ggt cac 1056

Glu Asn His Val Glu Ile Val Glu Glu Ala Gly Lys Leu Leu Gly His

340 345 350

tct ttg gac ttg gtt ttc tcc atc cac gct gac aag gaa gat ggt tct 1104

Ser Leu Asp Leu Val Phe Ser Ile His Ala Asp Lys Glu Asp Gly Ser

355 360 365

cca tta gaa tcc gcc gtc cca cct cca ttc cca ggt cca tgt acc tta 1152

Pro Leu Glu Ser Ala Val Pro Pro Pro Phe Pro Gly Pro Cys Thr Leu

370 375 380

ggt act ggt cta gct cgt tac gct gat ttg ttg aac cca cca aga aag 1200

Gly Thr Gly Leu Ala Arg Tyr Ala Asp Leu Leu Asn Pro Pro Arg Lys

385 390 395 400

tct gct ttg gtt gct ttg gct gct tac gct act gaa cct tct gaa gct 1248

Ser Ala Leu Val Ala Leu Ala Ala Tyr Ala Thr Glu Pro Ser Glu Ala

405 410 415

gaa aag ttg aag cat ttg act tct cca gac ggt aag gat gaa tac tcc 1296

Glu Lys Leu Lys His Leu Thr Ser Pro Asp Gly Lys Asp Glu Tyr Ser

420 425 430

caa tgg atc gtt gct tct caa aga tct cta ttg gaa gtt atg gct gct 1344

Gln Trp Ile Val Ala Ser Gln Arg Ser Leu Leu Glu Val Met Ala Ala

435 440 445

ttc cca tct gcc aag cca cca ttg ggt gtt ttc ttt gct gcc att gct 1392

Phe Pro Ser Ala Lys Pro Pro Leu Gly Val Phe Phe Ala Ala Ile Ala

450 455 460

cca aga ttg caa cca aga tac tac tcc att tct tct tct cca aga ttg 1440

Pro Arg Leu Gln Pro Arg Tyr Tyr Ser Ile Ser Ser Ser Pro Arg Leu

465 470 475 480

gct cca tct aga gtc cac gtc acc tct gct ttg gtt tac ggt cca act 1488

Ala Pro Ser Arg Val His Val Thr Ser Ala Leu Val Tyr Gly Pro Thr

485 490 495

cca acc ggt aga atc cac aaa ggt gtt tgt tcc acc tgg atg aag aac 1536

Pro Thr Gly Arg Ile His Lys Gly Val Cys Ser Thr Trp Met Lys Asn

500 505 510

gct gtt cca gct gaa aag tcc cac gaa tgt tcc ggt gct cca atc ttt 1584

Ala Val Pro Ala Glu Lys Ser His Glu Cys Ser Gly Ala Pro Ile Phe

515 520 525

atc aga gct tct aac ttc aag ttg cca tcc aac cca tct act cca atc 1632

Ile Arg Ala Ser Asn Phe Lys Leu Pro Ser Asn Pro Ser Thr Pro Ile

530 535 540

gtc atg gtt ggt cca ggt act ggt ttg gct cca ttc aga ggt ttc tta 1680

Val Met Val Gly Pro Gly Thr Gly Leu Ala Pro Phe Arg Gly Phe Leu

545 550 555 560

caa gaa aga atg gct ttg aaa gaa gat ggt gaa gaa ttg ggt tct tct 1728

Gln Glu Arg Met Ala Leu Lys Glu Asp Gly Glu Glu Leu Gly Ser Ser

565 570 575

cta ttg ttc ttc ggt tgt aga aac aga caa atg gac ttc atc tac gaa 1776

Leu Leu Phe Phe Gly Cys Arg Asn Arg Gln Met Asp Phe Ile Tyr Glu

580 585 590

gat gaa tta aac aac ttt gtc gac caa ggt gtt atc tcc gaa ttg atc 1824

Asp Glu Leu Asn Asn Phe Val Asp Gln Gly Val Ile Ser Glu Leu Ile

595 600 605

atg gcc ttc tct aga gaa ggt gct caa aag gaa tac gtt caa cat aaa 1872

Met Ala Phe Ser Arg Glu Gly Ala Gln Lys Glu Tyr Val Gln His Lys

610 615 620

atg atg gaa aag gct gct caa gtt tgg gac ttg atc aag gaa gaa ggt 1920

Met Met Glu Lys Ala Ala Gln Val Trp Asp Leu Ile Lys Glu Glu Gly

625 630 635 640

tac ttg tac gtt tgt ggt gat gcc aaa ggt atg gcc aga gat gtc cac 1968

Tyr Leu Tyr Val Cys Gly Asp Ala Lys Gly Met Ala Arg Asp Val His

645 650 655

aga act ttg cac acc att gtc caa gaa caa gaa ggt gtt tcc tct tcc 2016

Arg Thr Leu His Thr Ile Val Gln Glu Gln Glu Gly Val Ser Ser Ser

660 665 670

gaa gct gaa gcc att gtc aag aag tta caa act gaa ggt cgt tat ttg 2064

Glu Ala Glu Ala Ile Val Lys Lys Leu Gln Thr Glu Gly Arg Tyr Leu

675 680 685

aga gat gtc tgg 2076

Arg Asp Val Trp

690

<210> 56

<211> 692

<212> PRT

<213> Arabidopsis thaliana

<400> 56

Met Thr Ser Ala Leu Tyr Ala Ser Asp Leu Phe Lys Gln Leu Lys Ser

1 5 10 15

Ile Met Gly Thr Asp Ser Leu Ser Asp Asp Val Val Leu Val Ile Ala

20 25 30

Thr Thr Ser Leu Ala Leu Val Ala Gly Phe Val Val Leu Leu Trp Lys

35 40 45

Lys Thr Thr Ala Asp Arg Ser Gly Glu Leu Lys Pro Leu Met Ile Pro

50 55 60

Lys Ser Leu Met Ala Lys Asp Glu Asp Asp Asp Leu Asp Leu Gly Ser

65 70 75 80

Gly Lys Thr Arg Val Ser Ile Phe Phe Gly Thr Gln Thr Gly Thr Ala

85 90 95

Glu Gly Phe Ala Lys Ala Leu Ser Glu Glu Ile Lys Ala Arg Tyr Glu

100 105 110

Lys Ala Ala Val Lys Val Ile Asp Leu Asp Asp Tyr Ala Ala Asp Asp

115 120 125

Asp Gln Tyr Glu Glu Lys Leu Lys Lys Glu Thr Leu Ala Phe Phe Cys

130 135 140

Val Ala Thr Tyr Gly Asp Gly Glu Pro Thr Asp Asn Ala Ala Arg Phe

145 150 155 160

Tyr Lys Trp Phe Thr Glu Glu Asn Glu Arg Asp Ile Lys Leu Gln Gln

165 170 175

Leu Ala Tyr Gly Val Phe Ala Leu Gly Asn Arg Gln Tyr Glu His Phe

180 185 190

Asn Lys Ile Gly Ile Val Leu Asp Glu Glu Leu Cys Lys Lys Gly Ala

195 200 205

Lys Arg Leu Ile Glu Val Gly Leu Gly Asp Asp Asp Gln Ser Ile Glu

210 215 220

Asp Asp Phe Asn Ala Trp Lys Glu Ser Leu Trp Ser Glu Leu Asp Lys

225 230 235 240

Leu Leu Lys Asp Glu Asp Asp Lys Ser Val Ala Thr Pro Tyr Thr Ala

245 250 255

Val Ile Pro Glu Tyr Arg Val Val Thr His Asp Pro Arg Phe Thr Thr

260 265 270

Gln Lys Ser Met Glu Ser Asn Val Ala Asn Gly Asn Thr Thr Ile Asp

275 280 285

Ile His His Pro Cys Arg Val Asp Val Ala Val Gln Lys Glu Leu His

290 295 300

Thr His Glu Ser Asp Arg Ser Cys Ile His Leu Glu Phe Asp Ile Ser

305 310 315 320

Arg Thr Gly Ile Thr Tyr Glu Thr Gly Asp His Val Gly Val Tyr Ala

325 330 335

Glu Asn His Val Glu Ile Val Glu Glu Ala Gly Lys Leu Leu Gly His

340 345 350

Ser Leu Asp Leu Val Phe Ser Ile His Ala Asp Lys Glu Asp Gly Ser

355 360 365

Pro Leu Glu Ser Ala Val Pro Pro Pro Phe Pro Gly Pro Cys Thr Leu

370 375 380

Gly Thr Gly Leu Ala Arg Tyr Ala Asp Leu Leu Asn Pro Pro Arg Lys

385 390 395 400

Ser Ala Leu Val Ala Leu Ala Ala Tyr Ala Thr Glu Pro Ser Glu Ala

405 410 415

Glu Lys Leu Lys His Leu Thr Ser Pro Asp Gly Lys Asp Glu Tyr Ser

420 425 430

Gln Trp Ile Val Ala Ser Gln Arg Ser Leu Leu Glu Val Met Ala Ala

435 440 445

Phe Pro Ser Ala Lys Pro Pro Leu Gly Val Phe Phe Ala Ala Ile Ala

450 455 460

Pro Arg Leu Gln Pro Arg Tyr Tyr Ser Ile Ser Ser Ser Pro Arg Leu

465 470 475 480

Ala Pro Ser Arg Val His Val Thr Ser Ala Leu Val Tyr Gly Pro Thr

485 490 495

Pro Thr Gly Arg Ile His Lys Gly Val Cys Ser Thr Trp Met Lys Asn

500 505 510

Ala Val Pro Ala Glu Lys Ser His Glu Cys Ser Gly Ala Pro Ile Phe

515 520 525

Ile Arg Ala Ser Asn Phe Lys Leu Pro Ser Asn Pro Ser Thr Pro Ile

530 535 540

Val Met Val Gly Pro Gly Thr Gly Leu Ala Pro Phe Arg Gly Phe Leu

545 550 555 560

Gln Glu Arg Met Ala Leu Lys Glu Asp Gly Glu Glu Leu Gly Ser Ser

565 570 575

Leu Leu Phe Phe Gly Cys Arg Asn Arg Gln Met Asp Phe Ile Tyr Glu

580 585 590

Asp Glu Leu Asn Asn Phe Val Asp Gln Gly Val Ile Ser Glu Leu Ile

595 600 605

Met Ala Phe Ser Arg Glu Gly Ala Gln Lys Glu Tyr Val Gln His Lys

610 615 620

Met Met Glu Lys Ala Ala Gln Val Trp Asp Leu Ile Lys Glu Glu Gly

625 630 635 640

Tyr Leu Tyr Val Cys Gly Asp Ala Lys Gly Met Ala Arg Asp Val His

645 650 655

Arg Thr Leu His Thr Ile Val Gln Glu Gln Glu Gly Val Ser Ser Ser

660 665 670

Glu Ala Glu Ala Ile Val Lys Lys Leu Gln Thr Glu Gly Arg Tyr Leu

675 680 685

Arg Asp Val Trp

690

<210> 57

<211> 2133

<212> DNA

<213> Arabidopsis thaliana

<220>

<221> CDS

<222> (1)..(2133)

<400> 57

atg tcc tct tct tct tct tct tct act tcc atg att gat ttg atg gct 48

Met Ser Ser Ser Ser Ser Ser Ser Thr Ser Met Ile Asp Leu Met Ala

1 5 10 15

gcc atc atc aag ggt gaa cca gtc att gtc tct gac cca gcc aac gct 96

Ala Ile Ile Lys Gly Glu Pro Val Ile Val Ser Asp Pro Ala Asn Ala

20 25 30

tct gct tac gaa tcc gtt gct gct gaa ttg tcc tcc atg ttg att gaa 144

Ser Ala Tyr Glu Ser Val Ala Ala Glu Leu Ser Ser Met Leu Ile Glu

35 40 45

aac aga caa ttc gct atg att gtc act act tcc att gct gtc ttg att 192

Asn Arg Gln Phe Ala Met Ile Val Thr Thr Ser Ile Ala Val Leu Ile

50 55 60

ggt tgt atc gtc atg ttg gtc tgg aga aga tcc ggt tcc ggt aac tcc 240

Gly Cys Ile Val Met Leu Val Trp Arg Arg Ser Gly Ser Gly Asn Ser

65 70 75 80

aag aga gtt gaa cca ttg aag cca tta gtc atc aag cca aga gaa gaa 288

Lys Arg Val Glu Pro Leu Lys Pro Leu Val Ile Lys Pro Arg Glu Glu

85 90 95

gaa att gat gac ggt aga aag aag gtc acc atc ttc ttt ggt act caa 336

Glu Ile Asp Asp Gly Arg Lys Lys Val Thr Ile Phe Phe Gly Thr Gln

100 105 110

acc ggt act gct gaa ggt ttt gct aag gct ttg ggt gaa gaa gcc aaa 384

Thr Gly Thr Ala Glu Gly Phe Ala Lys Ala Leu Gly Glu Glu Ala Lys

115 120 125

gct aga tac gaa aag acc aga ttc aag atc gtt gac ttg gac gac tac 432

Ala Arg Tyr Glu Lys Thr Arg Phe Lys Ile Val Asp Leu Asp Asp Tyr

130 135 140

gct gct gat gac gac gaa tac gaa gaa aag ttg aag aag gaa gat gtt 480

Ala Ala Asp Asp Asp Glu Tyr Glu Glu Lys Leu Lys Lys Glu Asp Val

145 150 155 160

gcc ttc ttc ttc ttg gct act tac ggt gat ggt gaa cca act gac aat 528

Ala Phe Phe Phe Leu Ala Thr Tyr Gly Asp Gly Glu Pro Thr Asp Asn

165 170 175

gct gcc aga ttc tac aaa tgg ttc acc gaa ggt aac gac aga ggt gaa 576

Ala Ala Arg Phe Tyr Lys Trp Phe Thr Glu Gly Asn Asp Arg Gly Glu

180 185 190

tgg tta aag aac ttg aaa tac ggt gtt ttc ggt cta ggt aac aga caa 624

Trp Leu Lys Asn Leu Lys Tyr Gly Val Phe Gly Leu Gly Asn Arg Gln

195 200 205

tac gaa cac ttc aac aag gtt gcc aag gtt gtc gat gac atc ttg gtt 672

Tyr Glu His Phe Asn Lys Val Ala Lys Val Val Asp Asp Ile Leu Val

210 215 220

gaa caa ggt gct caa aga tta gtc caa gtc ggt ttg ggt gat gat gac 720

Glu Gln Gly Ala Gln Arg Leu Val Gln Val Gly Leu Gly Asp Asp Asp

225 230 235 240

caa tgt atc gaa gat gac ttc act gct tgg aga gaa gct ttg tgg cca 768

Gln Cys Ile Glu Asp Asp Phe Thr Ala Trp Arg Glu Ala Leu Trp Pro

245 250 255

gaa ttg gac acc atc tta aga gaa gaa ggt gat acc gct gtt gcc acc 816

Glu Leu Asp Thr Ile Leu Arg Glu Glu Gly Asp Thr Ala Val Ala Thr

260 265 270

cca tac act gct gct gtt ttg gaa tac aga gtt tct atc cac gac tct 864

Pro Tyr Thr Ala Ala Val Leu Glu Tyr Arg Val Ser Ile His Asp Ser

275 280 285

gaa gat gcc aag ttc aac gac atc aac atg gct aac ggt aac ggt tac 912

Glu Asp Ala Lys Phe Asn Asp Ile Asn Met Ala Asn Gly Asn Gly Tyr

290 295 300

act gtt ttc gac gct caa cac cca tac aag gcc aat gtt gct gtc aag 960

Thr Val Phe Asp Ala Gln His Pro Tyr Lys Ala Asn Val Ala Val Lys

305 310 315 320

aga gaa ttg cac act cca gaa tct gat cgt tct tgt atc cac ttg gaa 1008

Arg Glu Leu His Thr Pro Glu Ser Asp Arg Ser Cys Ile His Leu Glu

325 330 335

ttt gac att gct ggt tct ggt ttg acc tac gaa acc ggt gac cac gtc 1056

Phe Asp Ile Ala Gly Ser Gly Leu Thr Tyr Glu Thr Gly Asp His Val

340 345 350

ggt gtc tta tgt gac aac ttg tct gaa act gtc gat gaa gct ttg aga 1104

Gly Val Leu Cys Asp Asn Leu Ser Glu Thr Val Asp Glu Ala Leu Arg

355 360 365

tta ttg gac atg tct cca gac act tat ttc tcc ttg cat gct gaa aag 1152

Leu Leu Asp Met Ser Pro Asp Thr Tyr Phe Ser Leu His Ala Glu Lys

370 375 380

gaa gat ggt act cca att tct tct tcc ttg cct cct cca ttc cca cca 1200

Glu Asp Gly Thr Pro Ile Ser Ser Ser Leu Pro Pro Pro Phe Pro Pro

385 390 395 400

tgt aac ttg aga acc gct tta acc aga tac gct tgt ttg cta tcc tct 1248

Cys Asn Leu Arg Thr Ala Leu Thr Arg Tyr Ala Cys Leu Leu Ser Ser

405 410 415

cca aag aag tcc gct ttg gtt gct ttg gct gct cac gct tct gac cca 1296

Pro Lys Lys Ser Ala Leu Val Ala Leu Ala Ala His Ala Ser Asp Pro

420 425 430

act gaa gct gaa aga ttg aaa cat ttg gct tcc cca gct ggt aag gat 1344

Thr Glu Ala Glu Arg Leu Lys His Leu Ala Ser Pro Ala Gly Lys Asp

435 440 445

gaa tac tcc aaa tgg gtt gtt gaa tct caa aga tct ttg ttg gaa gtc 1392

Glu Tyr Ser Lys Trp Val Val Glu Ser Gln Arg Ser Leu Leu Glu Val

450 455 460

atg gct gaa ttc cca tct gcc aag cca cca ttg ggt gtt ttc ttc gcc 1440

Met Ala Glu Phe Pro Ser Ala Lys Pro Pro Leu Gly Val Phe Phe Ala

465 470 475 480

ggt gtt gct cca aga ttg caa cca aga ttt tac tcc atc tct tct tct 1488

Gly Val Ala Pro Arg Leu Gln Pro Arg Phe Tyr Ser Ile Ser Ser Ser

485 490 495

cca aag att gct gaa acc aga att cac gtt acc tgt gcc ttg gtc tac 1536

Pro Lys Ile Ala Glu Thr Arg Ile His Val Thr Cys Ala Leu Val Tyr

500 505 510

gaa aag atg cca acc ggt aga att cac aag ggt gtt tgt tcc acc tgg 1584

Glu Lys Met Pro Thr Gly Arg Ile His Lys Gly Val Cys Ser Thr Trp

515 520 525

atg aag aac gct gtt cca tac gaa aag tct gaa aac tgt tct tct gct 1632

Met Lys Asn Ala Val Pro Tyr Glu Lys Ser Glu Asn Cys Ser Ser Ala

530 535 540

cca atc ttc gtc cgt caa tcc aac ttc aag ttg cca tct gac tcc aag 1680

Pro Ile Phe Val Arg Gln Ser Asn Phe Lys Leu Pro Ser Asp Ser Lys

545 550 555 560

gtc cca atc atc atg atc ggt cca ggt act ggt tta gct cca ttc aga 1728

Val Pro Ile Ile Met Ile Gly Pro Gly Thr Gly Leu Ala Pro Phe Arg

565 570 575

ggt ttc ttg caa gaa aga ttg gcc tta gtt gaa tct ggt gtc gaa ttg 1776

Gly Phe Leu Gln Glu Arg Leu Ala Leu Val Glu Ser Gly Val Glu Leu

580 585 590

ggt cct tct gtt ttg ttc ttc ggt tgt aga aac cgt cgt atg gac ttc 1824

Gly Pro Ser Val Leu Phe Phe Gly Cys Arg Asn Arg Arg Met Asp Phe

595 600 605

atc tac gaa gaa gaa ttg caa aga ttt gtc gaa tct ggt gct ttg gct 1872

Ile Tyr Glu Glu Glu Leu Gln Arg Phe Val Glu Ser Gly Ala Leu Ala

610 615 620

gaa ttg tcc gtt gct ttc tct cgt gaa ggt cca acc aaa gaa tac gtt 1920

Glu Leu Ser Val Ala Phe Ser Arg Glu Gly Pro Thr Lys Glu Tyr Val

625 630 635 640

caa cac aag atg atg gac aaa gcc tcc gac atc tgg aac atg atc tcc 1968

Gln His Lys Met Met Asp Lys Ala Ser Asp Ile Trp Asn Met Ile Ser

645 650 655

caa ggt gct tac ttg tac gtt tgt ggt gat gct aaa ggt atg gcc aga 2016

Gln Gly Ala Tyr Leu Tyr Val Cys Gly Asp Ala Lys Gly Met Ala Arg

660 665 670

gat gtc cac aga tct tta cat acc att gcc caa gaa caa ggt tcc atg 2064

Asp Val His Arg Ser Leu His Thr Ile Ala Gln Glu Gln Gly Ser Met

675 680 685

gac tcc acc aag gct gaa ggt ttc gtt aag aac ttg caa act tct ggt 2112

Asp Ser Thr Lys Ala Glu Gly Phe Val Lys Asn Leu Gln Thr Ser Gly

690 695 700

cgt tac ttg aga gat gtt tgg 2133

Arg Tyr Leu Arg Asp Val Trp

705 710

<210> 58

<211> 711

<212> PRT

<213> Arabidopsis thaliana

<400> 58

Met Ser Ser Ser Ser Ser Ser Ser Thr Ser Met Ile Asp Leu Met Ala

1 5 10 15

Ala Ile Ile Lys Gly Glu Pro Val Ile Val Ser Asp Pro Ala Asn Ala

20 25 30

Ser Ala Tyr Glu Ser Val Ala Ala Glu Leu Ser Ser Met Leu Ile Glu

35 40 45

Asn Arg Gln Phe Ala Met Ile Val Thr Thr Ser Ile Ala Val Leu Ile

50 55 60

Gly Cys Ile Val Met Leu Val Trp Arg Arg Ser Gly Ser Gly Asn Ser

65 70 75 80

Lys Arg Val Glu Pro Leu Lys Pro Leu Val Ile Lys Pro Arg Glu Glu

85 90 95

Glu Ile Asp Asp Gly Arg Lys Lys Val Thr Ile Phe Phe Gly Thr Gln

100 105 110

Thr Gly Thr Ala Glu Gly Phe Ala Lys Ala Leu Gly Glu Glu Ala Lys

115 120 125

Ala Arg Tyr Glu Lys Thr Arg Phe Lys Ile Val Asp Leu Asp Asp Tyr

130 135 140

Ala Ala Asp Asp Asp Glu Tyr Glu Glu Lys Leu Lys Lys Glu Asp Val

145 150 155 160

Ala Phe Phe Phe Leu Ala Thr Tyr Gly Asp Gly Glu Pro Thr Asp Asn

165 170 175

Ala Ala Arg Phe Tyr Lys Trp Phe Thr Glu Gly Asn Asp Arg Gly Glu

180 185 190

Trp Leu Lys Asn Leu Lys Tyr Gly Val Phe Gly Leu Gly Asn Arg Gln

195 200 205

Tyr Glu His Phe Asn Lys Val Ala Lys Val Val Asp Asp Ile Leu Val

210 215 220

Glu Gln Gly Ala Gln Arg Leu Val Gln Val Gly Leu Gly Asp Asp Asp

225 230 235 240

Gln Cys Ile Glu Asp Asp Phe Thr Ala Trp Arg Glu Ala Leu Trp Pro

245 250 255

Glu Leu Asp Thr Ile Leu Arg Glu Glu Gly Asp Thr Ala Val Ala Thr

260 265 270

Pro Tyr Thr Ala Ala Val Leu Glu Tyr Arg Val Ser Ile His Asp Ser

275 280 285

Glu Asp Ala Lys Phe Asn Asp Ile Asn Met Ala Asn Gly Asn Gly Tyr

290 295 300

Thr Val Phe Asp Ala Gln His Pro Tyr Lys Ala Asn Val Ala Val Lys

305 310 315 320

Arg Glu Leu His Thr Pro Glu Ser Asp Arg Ser Cys Ile His Leu Glu

325 330 335

Phe Asp Ile Ala Gly Ser Gly Leu Thr Tyr Glu Thr Gly Asp His Val

340 345 350

Gly Val Leu Cys Asp Asn Leu Ser Glu Thr Val Asp Glu Ala Leu Arg

355 360 365

Leu Leu Asp Met Ser Pro Asp Thr Tyr Phe Ser Leu His Ala Glu Lys

370 375 380

Glu Asp Gly Thr Pro Ile Ser Ser Ser Leu Pro Pro Pro Phe Pro Pro

385 390 395 400

Cys Asn Leu Arg Thr Ala Leu Thr Arg Tyr Ala Cys Leu Leu Ser Ser

405 410 415

Pro Lys Lys Ser Ala Leu Val Ala Leu Ala Ala His Ala Ser Asp Pro

420 425 430

Thr Glu Ala Glu Arg Leu Lys His Leu Ala Ser Pro Ala Gly Lys Asp

435 440 445

Glu Tyr Ser Lys Trp Val Val Glu Ser Gln Arg Ser Leu Leu Glu Val

450 455 460

Met Ala Glu Phe Pro Ser Ala Lys Pro Pro Leu Gly Val Phe Phe Ala

465 470 475 480

Gly Val Ala Pro Arg Leu Gln Pro Arg Phe Tyr Ser Ile Ser Ser Ser

485 490 495

Pro Lys Ile Ala Glu Thr Arg Ile His Val Thr Cys Ala Leu Val Tyr

500 505 510

Glu Lys Met Pro Thr Gly Arg Ile His Lys Gly Val Cys Ser Thr Trp

515 520 525

Met Lys Asn Ala Val Pro Tyr Glu Lys Ser Glu Asn Cys Ser Ser Ala

530 535 540

Pro Ile Phe Val Arg Gln Ser Asn Phe Lys Leu Pro Ser Asp Ser Lys

545 550 555 560

Val Pro Ile Ile Met Ile Gly Pro Gly Thr Gly Leu Ala Pro Phe Arg

565 570 575

Gly Phe Leu Gln Glu Arg Leu Ala Leu Val Glu Ser Gly Val Glu Leu

580 585 590

Gly Pro Ser Val Leu Phe Phe Gly Cys Arg Asn Arg Arg Met Asp Phe

595 600 605

Ile Tyr Glu Glu Glu Leu Gln Arg Phe Val Glu Ser Gly Ala Leu Ala

610 615 620

Glu Leu Ser Val Ala Phe Ser Arg Glu Gly Pro Thr Lys Glu Tyr Val

625 630 635 640

Gln His Lys Met Met Asp Lys Ala Ser Asp Ile Trp Asn Met Ile Ser

645 650 655

Gln Gly Ala Tyr Leu Tyr Val Cys Gly Asp Ala Lys Gly Met Ala Arg

660 665 670

Asp Val His Arg Ser Leu His Thr Ile Ala Gln Glu Gln Gly Ser Met

675 680 685

Asp Ser Thr Lys Ala Glu Gly Phe Val Lys Asn Leu Gln Thr Ser Gly

690 695 700

Arg Tyr Leu Arg Asp Val Trp

705 710

<210> 59

<211> 2361

<212> DNA

<213> Stevia rebaudiana

<220>

<221> CDS

<222> (1)..(2361)

<400> 59

atg aag act ggt ttc atc tct cca gct acc gtc ttc cac cac aga atc 48

Met Lys Thr Gly Phe Ile Ser Pro Ala Thr Val Phe His His Arg Ile

1 5 10 15

tct cca gct acc act ttc aga cac cac ttg tcc cca gct acc act aac 96

Ser Pro Ala Thr Thr Phe Arg His His Leu Ser Pro Ala Thr Thr Asn

20 25 30

tct act ggt att gtt gct ttg aga gac atc aac ttc aga tgt aaa gct 144

Ser Thr Gly Ile Val Ala Leu Arg Asp Ile Asn Phe Arg Cys Lys Ala

35 40 45

gtt tcc aag gaa tac tct gac ttg ttg caa aag gat gaa gcc tcc ttc 192

Val Ser Lys Glu Tyr Ser Asp Leu Leu Gln Lys Asp Glu Ala Ser Phe

50 55 60

acc aaa tgg gat gat gac aaa gtt aag gac cat tta gac act aac aag 240

Thr Lys Trp Asp Asp Asp Lys Val Lys Asp His Leu Asp Thr Asn Lys

65 70 75 80

aac ttg tac cca aac gat gaa atc aag gaa ttc gtc gaa tct gtc aaa 288

Asn Leu Tyr Pro Asn Asp Glu Ile Lys Glu Phe Val Glu Ser Val Lys

85 90 95

gct atg ttc ggt tcc atg aat gat ggt gaa atc aac gtt tcc gct tac 336

Ala Met Phe Gly Ser Met Asn Asp Gly Glu Ile Asn Val Ser Ala Tyr

100 105 110

gac acc gct tgg gtt gct ttg gtt caa gac gtt gat ggt tcc ggt tct 384

Asp Thr Ala Trp Val Ala Leu Val Gln Asp Val Asp Gly Ser Gly Ser

115 120 125

cca caa ttc cca tct tct ttg gaa tgg att gcc aac aac caa ttg tct 432

Pro Gln Phe Pro Ser Ser Leu Glu Trp Ile Ala Asn Asn Gln Leu Ser

130 135 140

gat ggt tct tgg ggt gac cat ttg tta ttc tct gct cac gac aga att 480

Asp Gly Ser Trp Gly Asp His Leu Leu Phe Ser Ala His Asp Arg Ile

145 150 155 160

att aac act tta gct tgt gtc att gct ttg act tcc tgg aat gtc cat 528

Ile Asn Thr Leu Ala Cys Val Ile Ala Leu Thr Ser Trp Asn Val His

165 170 175

cca tcc aag tgt gaa aag ggt ttg aac ttc ttg aga gaa aac atc tgt 576

Pro Ser Lys Cys Glu Lys Gly Leu Asn Phe Leu Arg Glu Asn Ile Cys

180 185 190

aag ttg gaa gat gaa aat gct gaa cac atg cca att ggt ttc gaa gtt 624

Lys Leu Glu Asp Glu Asn Ala Glu His Met Pro Ile Gly Phe Glu Val

195 200 205

acc ttc cca tct ttg att gat atc gcc aag aag ttg aac atc gaa gtc 672

Thr Phe Pro Ser Leu Ile Asp Ile Ala Lys Lys Leu Asn Ile Glu Val

210 215 220

cca gaa gac acc cca gct ttg aag gaa atc tac gcc aga aga gat atc 720

Pro Glu Asp Thr Pro Ala Leu Lys Glu Ile Tyr Ala Arg Arg Asp Ile

225 230 235 240

aag ttg acc aaa atc cca atg gaa gtt ttg cac aag gtt cca acc acc 768

Lys Leu Thr Lys Ile Pro Met Glu Val Leu His Lys Val Pro Thr Thr

245 250 255

ttg ttg cac tct ttg gaa ggt atg cca gac ttg gaa tgg gaa aag ttg 816

Leu Leu His Ser Leu Glu Gly Met Pro Asp Leu Glu Trp Glu Lys Leu

260 265 270

tta aag ttg caa tgt aag gac ggt tct ttc ttg ttc tct cca tct tct 864

Leu Lys Leu Gln Cys Lys Asp Gly Ser Phe Leu Phe Ser Pro Ser Ser

275 280 285

acc gcc ttt gct ttg atg caa act aag gac gaa aag tgt cta caa tac 912

Thr Ala Phe Ala Leu Met Gln Thr Lys Asp Glu Lys Cys Leu Gln Tyr

290 295 300

tta act aat atc gtt acc aaa ttc aac ggt ggt gtc cca aac gtt tac 960

Leu Thr Asn Ile Val Thr Lys Phe Asn Gly Gly Val Pro Asn Val Tyr

305 310 315 320

cct gtt gac ttg ttt gaa cac atc tgg gtt gtt gac aga ttg caa cgt 1008

Pro Val Asp Leu Phe Glu His Ile Trp Val Val Asp Arg Leu Gln Arg

325 330 335

ttg ggt att gct cgt tat ttc aag tct gaa atc aag gac tgt gtt gaa 1056

Leu Gly Ile Ala Arg Tyr Phe Lys Ser Glu Ile Lys Asp Cys Val Glu

340 345 350

tac atc aac aag tac tgg act aag aac ggt atc tgt tgg gct cgt aac 1104

Tyr Ile Asn Lys Tyr Trp Thr Lys Asn Gly Ile Cys Trp Ala Arg Asn

355 360 365

acc cac gtt caa gat atc gac gac act gct atg ggt ttc aga gtc ttg 1152

Thr His Val Gln Asp Ile Asp Asp Thr Ala Met Gly Phe Arg Val Leu

370 375 380

aga gct cat ggt tac gat gtc acc cca gat gtc ttc aga caa ttc gaa 1200

Arg Ala His Gly Tyr Asp Val Thr Pro Asp Val Phe Arg Gln Phe Glu

385 390 395 400

aag gat ggt aag ttc gtt tgt ttt gcc ggt caa tcc act caa gcc gtc 1248

Lys Asp Gly Lys Phe Val Cys Phe Ala Gly Gln Ser Thr Gln Ala Val

405 410 415

act ggt atg ttc aac gtc tac aga gct tct caa atg ttg ttc cca ggt 1296

Thr Gly Met Phe Asn Val Tyr Arg Ala Ser Gln Met Leu Phe Pro Gly

420 425 430

gaa aga atc cta gaa gac gct aag aag ttc tcc tac aac tac ttg aaa 1344

Glu Arg Ile Leu Glu Asp Ala Lys Lys Phe Ser Tyr Asn Tyr Leu Lys

435 440 445

gaa aag caa tct act aac gaa ttg ttg gac aaa tgg atc att gcc aaa 1392

Glu Lys Gln Ser Thr Asn Glu Leu Leu Asp Lys Trp Ile Ile Ala Lys

450 455 460

gac tta cca ggt gaa gtc ggt tac gct ttg gat att cca tgg tac gct 1440

Asp Leu Pro Gly Glu Val Gly Tyr Ala Leu Asp Ile Pro Trp Tyr Ala

465 470 475 480

tct cta cca aga tta gaa acc aga tac tac ttg gaa caa tac ggt ggt 1488

Ser Leu Pro Arg Leu Glu Thr Arg Tyr Tyr Leu Glu Gln Tyr Gly Gly

485 490 495

gaa gac gat gtc tgg atc ggt aag acc ttg tac aga atg ggt tac gtt 1536

Glu Asp Asp Val Trp Ile Gly Lys Thr Leu Tyr Arg Met Gly Tyr Val

500 505 510

tcc aac aac act tac ttg gaa atg gcc aaa ttg gac tac aac aac tac 1584

Ser Asn Asn Thr Tyr Leu Glu Met Ala Lys Leu Asp Tyr Asn Asn Tyr

515 520 525

gtc gcc gtc tta caa ttg gaa tgg tac acc att caa caa tgg tac gtt 1632

Val Ala Val Leu Gln Leu Glu Trp Tyr Thr Ile Gln Gln Trp Tyr Val

530 535 540

gac att ggt att gaa aag ttt gaa tcc gac aac atc aag tcc gtc ttg 1680

Asp Ile Gly Ile Glu Lys Phe Glu Ser Asp Asn Ile Lys Ser Val Leu

545 550 555 560

gtt tcc tac tac ttg gct gct gct tcc atc ttt gaa cca gaa aga tcc 1728

Val Ser Tyr Tyr Leu Ala Ala Ala Ser Ile Phe Glu Pro Glu Arg Ser

565 570 575

aag gaa aga att gct tgg gct aag acc acc atc ttg gtt gac aag atc 1776

Lys Glu Arg Ile Ala Trp Ala Lys Thr Thr Ile Leu Val Asp Lys Ile

580 585 590

act tct att ttc gac tct tcc caa tct tcc aag gaa gat atc acc gct 1824

Thr Ser Ile Phe Asp Ser Ser Gln Ser Ser Lys Glu Asp Ile Thr Ala

595 600 605

ttc att gac aaa ttc aga aac aag tct tct tcc aag aag cac tcc att 1872

Phe Ile Asp Lys Phe Arg Asn Lys Ser Ser Ser Lys Lys His Ser Ile

610 615 620

aac ggt gaa cca tgg cac gaa gtt atg gtt gct ttg aag aag act ttg 1920

Asn Gly Glu Pro Trp His Glu Val Met Val Ala Leu Lys Lys Thr Leu

625 630 635 640

cac ggt ttt gct ttg gat gct ttg atg act cac tct caa gat att cac 1968

His Gly Phe Ala Leu Asp Ala Leu Met Thr His Ser Gln Asp Ile His

645 650 655

cct caa tta cac caa gct tgg gaa atg tgg tta acc aag ttg caa gat 2016

Pro Gln Leu His Gln Ala Trp Glu Met Trp Leu Thr Lys Leu Gln Asp

660 665 670

ggt gtc gat gtc act gct gaa ttg atg gtt caa atg atc aac atg act 2064

Gly Val Asp Val Thr Ala Glu Leu Met Val Gln Met Ile Asn Met Thr

675 680 685

gcc ggt aga tgg gtt tct aag gaa ttg ttg act cac cct caa tac caa 2112

Ala Gly Arg Trp Val Ser Lys Glu Leu Leu Thr His Pro Gln Tyr Gln

690 695 700

cgt ttg tcc acc gtc acc aac tct gtc tgt cac gac atc act aag ttg 2160

Arg Leu Ser Thr Val Thr Asn Ser Val Cys His Asp Ile Thr Lys Leu

705 710 715 720

cac aac ttc aaa gaa aac tcc act act gtc gat tct aag gtt caa gaa 2208

His Asn Phe Lys Glu Asn Ser Thr Thr Val Asp Ser Lys Val Gln Glu

725 730 735

ttg gtt caa tta gtt ttc tct gac acc cca gat gac ttg gac caa gac 2256

Leu Val Gln Leu Val Phe Ser Asp Thr Pro Asp Asp Leu Asp Gln Asp

740 745 750

atg aag caa act ttc ttg act gtc atg aag acc ttc tac tac aag gct 2304

Met Lys Gln Thr Phe Leu Thr Val Met Lys Thr Phe Tyr Tyr Lys Ala

755 760 765

tgg tgt gac cca aac acc atc aac gac cat att tct aag gtc ttc gaa 2352

Trp Cys Asp Pro Asn Thr Ile Asn Asp His Ile Ser Lys Val Phe Glu

770 775 780

att gtt atc 2361

Ile Val Ile

785

<210> 60

<211> 787

<212> PRT

<213> Stevia rebaudiana

<400> 60

Met Lys Thr Gly Phe Ile Ser Pro Ala Thr Val Phe His His Arg Ile

1 5 10 15

Ser Pro Ala Thr Thr Phe Arg His His Leu Ser Pro Ala Thr Thr Asn

20 25 30

Ser Thr Gly Ile Val Ala Leu Arg Asp Ile Asn Phe Arg Cys Lys Ala

35 40 45

Val Ser Lys Glu Tyr Ser Asp Leu Leu Gln Lys Asp Glu Ala Ser Phe

50 55 60

Thr Lys Trp Asp Asp Asp Lys Val Lys Asp His Leu Asp Thr Asn Lys

65 70 75 80

Asn Leu Tyr Pro Asn Asp Glu Ile Lys Glu Phe Val Glu Ser Val Lys

85 90 95

Ala Met Phe Gly Ser Met Asn Asp Gly Glu Ile Asn Val Ser Ala Tyr

100 105 110

Asp Thr Ala Trp Val Ala Leu Val Gln Asp Val Asp Gly Ser Gly Ser

115 120 125

Pro Gln Phe Pro Ser Ser Leu Glu Trp Ile Ala Asn Asn Gln Leu Ser

130 135 140

Asp Gly Ser Trp Gly Asp His Leu Leu Phe Ser Ala His Asp Arg Ile

145 150 155 160

Ile Asn Thr Leu Ala Cys Val Ile Ala Leu Thr Ser Trp Asn Val His

165 170 175

Pro Ser Lys Cys Glu Lys Gly Leu Asn Phe Leu Arg Glu Asn Ile Cys

180 185 190

Lys Leu Glu Asp Glu Asn Ala Glu His Met Pro Ile Gly Phe Glu Val

195 200 205

Thr Phe Pro Ser Leu Ile Asp Ile Ala Lys Lys Leu Asn Ile Glu Val

210 215 220

Pro Glu Asp Thr Pro Ala Leu Lys Glu Ile Tyr Ala Arg Arg Asp Ile

225 230 235 240

Lys Leu Thr Lys Ile Pro Met Glu Val Leu His Lys Val Pro Thr Thr

245 250 255

Leu Leu His Ser Leu Glu Gly Met Pro Asp Leu Glu Trp Glu Lys Leu

260 265 270

Leu Lys Leu Gln Cys Lys Asp Gly Ser Phe Leu Phe Ser Pro Ser Ser

275 280 285

Thr Ala Phe Ala Leu Met Gln Thr Lys Asp Glu Lys Cys Leu Gln Tyr

290 295 300

Leu Thr Asn Ile Val Thr Lys Phe Asn Gly Gly Val Pro Asn Val Tyr

305 310 315 320

Pro Val Asp Leu Phe Glu His Ile Trp Val Val Asp Arg Leu Gln Arg

325 330 335

Leu Gly Ile Ala Arg Tyr Phe Lys Ser Glu Ile Lys Asp Cys Val Glu

340 345 350

Tyr Ile Asn Lys Tyr Trp Thr Lys Asn Gly Ile Cys Trp Ala Arg Asn

355 360 365

Thr His Val Gln Asp Ile Asp Asp Thr Ala Met Gly Phe Arg Val Leu

370 375 380

Arg Ala His Gly Tyr Asp Val Thr Pro Asp Val Phe Arg Gln Phe Glu

385 390 395 400

Lys Asp Gly Lys Phe Val Cys Phe Ala Gly Gln Ser Thr Gln Ala Val

405 410 415

Thr Gly Met Phe Asn Val Tyr Arg Ala Ser Gln Met Leu Phe Pro Gly

420 425 430

Glu Arg Ile Leu Glu Asp Ala Lys Lys Phe Ser Tyr Asn Tyr Leu Lys

435 440 445

Glu Lys Gln Ser Thr Asn Glu Leu Leu Asp Lys Trp Ile Ile Ala Lys

450 455 460

Asp Leu Pro Gly Glu Val Gly Tyr Ala Leu Asp Ile Pro Trp Tyr Ala

465 470 475 480

Ser Leu Pro Arg Leu Glu Thr Arg Tyr Tyr Leu Glu Gln Tyr Gly Gly

485 490 495

Glu Asp Asp Val Trp Ile Gly Lys Thr Leu Tyr Arg Met Gly Tyr Val

500 505 510

Ser Asn Asn Thr Tyr Leu Glu Met Ala Lys Leu Asp Tyr Asn Asn Tyr

515 520 525

Val Ala Val Leu Gln Leu Glu Trp Tyr Thr Ile Gln Gln Trp Tyr Val

530 535 540

Asp Ile Gly Ile Glu Lys Phe Glu Ser Asp Asn Ile Lys Ser Val Leu

545 550 555 560

Val Ser Tyr Tyr Leu Ala Ala Ala Ser Ile Phe Glu Pro Glu Arg Ser

565 570 575

Lys Glu Arg Ile Ala Trp Ala Lys Thr Thr Ile Leu Val Asp Lys Ile

580 585 590

Thr Ser Ile Phe Asp Ser Ser Gln Ser Ser Lys Glu Asp Ile Thr Ala

595 600 605

Phe Ile Asp Lys Phe Arg Asn Lys Ser Ser Ser Lys Lys His Ser Ile

610 615 620

Asn Gly Glu Pro Trp His Glu Val Met Val Ala Leu Lys Lys Thr Leu

625 630 635 640

His Gly Phe Ala Leu Asp Ala Leu Met Thr His Ser Gln Asp Ile His

645 650 655

Pro Gln Leu His Gln Ala Trp Glu Met Trp Leu Thr Lys Leu Gln Asp

660 665 670

Gly Val Asp Val Thr Ala Glu Leu Met Val Gln Met Ile Asn Met Thr

675 680 685

Ala Gly Arg Trp Val Ser Lys Glu Leu Leu Thr His Pro Gln Tyr Gln

690 695 700

Arg Leu Ser Thr Val Thr Asn Ser Val Cys His Asp Ile Thr Lys Leu

705 710 715 720

His Asn Phe Lys Glu Asn Ser Thr Thr Val Asp Ser Lys Val Gln Glu

725 730 735

Leu Val Gln Leu Val Phe Ser Asp Thr Pro Asp Asp Leu Asp Gln Asp

740 745 750

Met Lys Gln Thr Phe Leu Thr Val Met Lys Thr Phe Tyr Tyr Lys Ala

755 760 765

Trp Cys Asp Pro Asn Thr Ile Asn Asp His Ile Ser Lys Val Phe Glu

770 775 780

Ile Val Ile

785

<210> 61

<211> 2229

<212> DNA

<213> Stevia rebaudiana

<220>

<221> CDS

<222> (1)..(2229)

<400> 61

atg tgt aaa gct gtt tcc aag gaa tac tct gac ttg ttg caa aag gat 48

Met Cys Lys Ala Val Ser Lys Glu Tyr Ser Asp Leu Leu Gln Lys Asp

1 5 10 15

gaa gcc tcc ttc acc aaa tgg gat gat gac aaa gtt aag gac cat tta 96

Glu Ala Ser Phe Thr Lys Trp Asp Asp Asp Lys Val Lys Asp His Leu

20 25 30

gac act aac aag aac ttg tac cca aac gat gaa atc aag gaa ttc gtc 144

Asp Thr Asn Lys Asn Leu Tyr Pro Asn Asp Glu Ile Lys Glu Phe Val

35 40 45

gaa tct gtc aaa gct atg ttc ggt tcc atg aat gat ggt gaa atc aac 192

Glu Ser Val Lys Ala Met Phe Gly Ser Met Asn Asp Gly Glu Ile Asn

50 55 60

gtt tcc gct tac gac acc gct tgg gtt gct ttg gtt caa gac gtt gat 240

Val Ser Ala Tyr Asp Thr Ala Trp Val Ala Leu Val Gln Asp Val Asp

65 70 75 80

ggt tcc ggt tct cca caa ttc cca tct tct ttg gaa tgg att gcc aac 288

Gly Ser Gly Ser Pro Gln Phe Pro Ser Ser Leu Glu Trp Ile Ala Asn

85 90 95

aac caa ttg tct gat ggt tct tgg ggt gac cat ttg tta ttc tct gct 336

Asn Gln Leu Ser Asp Gly Ser Trp Gly Asp His Leu Leu Phe Ser Ala

100 105 110

cac gac aga att att aac act tta gct tgt gtc att gct ttg act tcc 384

His Asp Arg Ile Ile Asn Thr Leu Ala Cys Val Ile Ala Leu Thr Ser

115 120 125

tgg aat gtc cat cca tcc aag tgt gaa aag ggt ttg aac ttc ttg aga 432

Trp Asn Val His Pro Ser Lys Cys Glu Lys Gly Leu Asn Phe Leu Arg

130 135 140

gaa aac atc tgt aag ttg gaa gat gaa aat gct gaa cac atg cca att 480

Glu Asn Ile Cys Lys Leu Glu Asp Glu Asn Ala Glu His Met Pro Ile

145 150 155 160

ggt ttc gaa gtt acc ttc cca tct ttg att gat atc gcc aag aag ttg 528

Gly Phe Glu Val Thr Phe Pro Ser Leu Ile Asp Ile Ala Lys Lys Leu

165 170 175

aac atc gaa gtc cca gaa gac acc cca gct ttg aag gaa atc tac gcc 576

Asn Ile Glu Val Pro Glu Asp Thr Pro Ala Leu Lys Glu Ile Tyr Ala

180 185 190

aga aga gat atc aag ttg acc aaa atc cca atg gaa gtt ttg cac aag 624

Arg Arg Asp Ile Lys Leu Thr Lys Ile Pro Met Glu Val Leu His Lys

195 200 205

gtt cca acc acc ttg ttg cac tct ttg gaa ggt atg cca gac ttg gaa 672

Val Pro Thr Thr Leu Leu His Ser Leu Glu Gly Met Pro Asp Leu Glu

210 215 220

tgg gaa aag ttg tta aag ttg caa tgt aag gac ggt tct ttc ttg ttc 720

Trp Glu Lys Leu Leu Lys Leu Gln Cys Lys Asp Gly Ser Phe Leu Phe

225 230 235 240

tct cca tct tct acc gcc ttt gct ttg atg caa act aag gac gaa aag 768

Ser Pro Ser Ser Thr Ala Phe Ala Leu Met Gln Thr Lys Asp Glu Lys

245 250 255

tgt cta caa tac tta act aat atc gtt acc aaa ttc aac ggt ggt gtc 816

Cys Leu Gln Tyr Leu Thr Asn Ile Val Thr Lys Phe Asn Gly Gly Val

260 265 270

cca aac gtt tac cct gtt gac ttg ttt gaa cac atc tgg gtt gtt gac 864

Pro Asn Val Tyr Pro Val Asp Leu Phe Glu His Ile Trp Val Val Asp

275 280 285

aga ttg caa cgt ttg ggt att gct cgt tat ttc aag tct gaa atc aag 912

Arg Leu Gln Arg Leu Gly Ile Ala Arg Tyr Phe Lys Ser Glu Ile Lys

290 295 300

gac tgt gtt gaa tac atc aac aag tac tgg act aag aac ggt atc tgt 960

Asp Cys Val Glu Tyr Ile Asn Lys Tyr Trp Thr Lys Asn Gly Ile Cys

305 310 315 320

tgg gct cgt aac acc cac gtt caa gat atc gac gac act gct atg ggt 1008

Trp Ala Arg Asn Thr His Val Gln Asp Ile Asp Asp Thr Ala Met Gly

325 330 335

ttc aga gtc ttg aga gct cat ggt tac gat gtc acc cca gat gtc ttc 1056

Phe Arg Val Leu Arg Ala His Gly Tyr Asp Val Thr Pro Asp Val Phe

340 345 350

aga caa ttc gaa aag gat ggt aag ttc gtt tgt ttt gcc ggt caa tcc 1104

Arg Gln Phe Glu Lys Asp Gly Lys Phe Val Cys Phe Ala Gly Gln Ser

355 360 365

act caa gcc gtc act ggt atg ttc aac gtc tac aga gct tct caa atg 1152

Thr Gln Ala Val Thr Gly Met Phe Asn Val Tyr Arg Ala Ser Gln Met

370 375 380

ttg ttc cca ggt gaa aga atc cta gaa gac gct aag aag ttc tcc tac 1200

Leu Phe Pro Gly Glu Arg Ile Leu Glu Asp Ala Lys Lys Phe Ser Tyr

385 390 395 400

aac tac ttg aaa gaa aag caa tct act aac gaa ttg ttg gac aaa tgg 1248

Asn Tyr Leu Lys Glu Lys Gln Ser Thr Asn Glu Leu Leu Asp Lys Trp

405 410 415

atc att gcc aaa gac tta cca ggt gaa gtc ggt tac gct ttg gat att 1296

Ile Ile Ala Lys Asp Leu Pro Gly Glu Val Gly Tyr Ala Leu Asp Ile

420 425 430

cca tgg tac gct tct cta cca aga tta gaa acc aga tac tac ttg gaa 1344

Pro Trp Tyr Ala Ser Leu Pro Arg Leu Glu Thr Arg Tyr Tyr Leu Glu

435 440 445

caa tac ggt ggt gaa gac gat gtc tgg atc ggt aag acc ttg tac aga 1392

Gln Tyr Gly Gly Glu Asp Asp Val Trp Ile Gly Lys Thr Leu Tyr Arg

450 455 460

atg ggt tac gtt tcc aac aac act tac ttg gaa atg gcc aaa ttg gac 1440

Met Gly Tyr Val Ser Asn Asn Thr Tyr Leu Glu Met Ala Lys Leu Asp

465 470 475 480

tac aac aac tac gtc gcc gtc tta caa ttg gaa tgg tac acc att caa 1488

Tyr Asn Asn Tyr Val Ala Val Leu Gln Leu Glu Trp Tyr Thr Ile Gln

485 490 495

caa tgg tac gtt gac att ggt att gaa aag ttt gaa tcc gac aac atc 1536

Gln Trp Tyr Val Asp Ile Gly Ile Glu Lys Phe Glu Ser Asp Asn Ile

500 505 510

aag tcc gtc ttg gtt tcc tac tac ttg gct gct gct tcc atc ttt gaa 1584

Lys Ser Val Leu Val Ser Tyr Tyr Leu Ala Ala Ala Ser Ile Phe Glu

515 520 525

cca gaa aga tcc aag gaa aga att gct tgg gct aag acc acc atc ttg 1632

Pro Glu Arg Ser Lys Glu Arg Ile Ala Trp Ala Lys Thr Thr Ile Leu

530 535 540

gtt gac aag atc act tct att ttc gac tct tcc caa tct tcc aag gaa 1680

Val Asp Lys Ile Thr Ser Ile Phe Asp Ser Ser Gln Ser Ser Lys Glu

545 550 555 560

gat atc acc gct ttc att gac aaa ttc aga aac aag tct tct tcc aag 1728

Asp Ile Thr Ala Phe Ile Asp Lys Phe Arg Asn Lys Ser Ser Ser Lys

565 570 575

aag cac tcc att aac ggt gaa cca tgg cac gaa gtt atg gtt gct ttg 1776

Lys His Ser Ile Asn Gly Glu Pro Trp His Glu Val Met Val Ala Leu

580 585 590

aag aag act ttg cac ggt ttt gct ttg gat gct ttg atg act cac tct 1824

Lys Lys Thr Leu His Gly Phe Ala Leu Asp Ala Leu Met Thr His Ser

595 600 605

caa gat att cac cct caa tta cac caa gct tgg gaa atg tgg tta acc 1872

Gln Asp Ile His Pro Gln Leu His Gln Ala Trp Glu Met Trp Leu Thr

610 615 620

aag ttg caa gat ggt gtc gat gtc act gct gaa ttg atg gtt caa atg 1920

Lys Leu Gln Asp Gly Val Asp Val Thr Ala Glu Leu Met Val Gln Met

625 630 635 640

atc aac atg act gcc ggt aga tgg gtt tct aag gaa ttg ttg act cac 1968

Ile Asn Met Thr Ala Gly Arg Trp Val Ser Lys Glu Leu Leu Thr His

645 650 655

cct caa tac caa cgt ttg tcc acc gtc acc aac tct gtc tgt cac gac 2016

Pro Gln Tyr Gln Arg Leu Ser Thr Val Thr Asn Ser Val Cys His Asp

660 665 670

atc act aag ttg cac aac ttc aaa gaa aac tcc act act gtc gat tct 2064

Ile Thr Lys Leu His Asn Phe Lys Glu Asn Ser Thr Thr Val Asp Ser

675 680 685

aag gtt caa gaa ttg gtt caa tta gtt ttc tct gac acc cca gat gac 2112

Lys Val Gln Glu Leu Val Gln Leu Val Phe Ser Asp Thr Pro Asp Asp

690 695 700

ttg gac caa gac atg aag caa act ttc ttg act gtc atg aag acc ttc 2160

Leu Asp Gln Asp Met Lys Gln Thr Phe Leu Thr Val Met Lys Thr Phe

705 710 715 720

tac tac aag gct tgg tgt gac cca aac acc atc aac gac cat att tct 2208

Tyr Tyr Lys Ala Trp Cys Asp Pro Asn Thr Ile Asn Asp His Ile Ser

725 730 735

aag gtc ttc gaa att gtt atc 2229

Lys Val Phe Glu Ile Val Ile

740

<210> 62

<211> 743

<212> PRT

<213> Stevia rebaudiana

<400> 62

Met Cys Lys Ala Val Ser Lys Glu Tyr Ser Asp Leu Leu Gln Lys Asp

1 5 10 15

Glu Ala Ser Phe Thr Lys Trp Asp Asp Asp Lys Val Lys Asp His Leu

20 25 30

Asp Thr Asn Lys Asn Leu Tyr Pro Asn Asp Glu Ile Lys Glu Phe Val

35 40 45

Glu Ser Val Lys Ala Met Phe Gly Ser Met Asn Asp Gly Glu Ile Asn

50 55 60

Val Ser Ala Tyr Asp Thr Ala Trp Val Ala Leu Val Gln Asp Val Asp

65 70 75 80

Gly Ser Gly Ser Pro Gln Phe Pro Ser Ser Leu Glu Trp Ile Ala Asn

85 90 95

Asn Gln Leu Ser Asp Gly Ser Trp Gly Asp His Leu Leu Phe Ser Ala

100 105 110

His Asp Arg Ile Ile Asn Thr Leu Ala Cys Val Ile Ala Leu Thr Ser

115 120 125

Trp Asn Val His Pro Ser Lys Cys Glu Lys Gly Leu Asn Phe Leu Arg

130 135 140

Glu Asn Ile Cys Lys Leu Glu Asp Glu Asn Ala Glu His Met Pro Ile

145 150 155 160

Gly Phe Glu Val Thr Phe Pro Ser Leu Ile Asp Ile Ala Lys Lys Leu

165 170 175

Asn Ile Glu Val Pro Glu Asp Thr Pro Ala Leu Lys Glu Ile Tyr Ala

180 185 190

Arg Arg Asp Ile Lys Leu Thr Lys Ile Pro Met Glu Val Leu His Lys

195 200 205

Val Pro Thr Thr Leu Leu His Ser Leu Glu Gly Met Pro Asp Leu Glu

210 215 220

Trp Glu Lys Leu Leu Lys Leu Gln Cys Lys Asp Gly Ser Phe Leu Phe

225 230 235 240

Ser Pro Ser Ser Thr Ala Phe Ala Leu Met Gln Thr Lys Asp Glu Lys

245 250 255

Cys Leu Gln Tyr Leu Thr Asn Ile Val Thr Lys Phe Asn Gly Gly Val

260 265 270

Pro Asn Val Tyr Pro Val Asp Leu Phe Glu His Ile Trp Val Val Asp

275 280 285

Arg Leu Gln Arg Leu Gly Ile Ala Arg Tyr Phe Lys Ser Glu Ile Lys

290 295 300

Asp Cys Val Glu Tyr Ile Asn Lys Tyr Trp Thr Lys Asn Gly Ile Cys

305 310 315 320

Trp Ala Arg Asn Thr His Val Gln Asp Ile Asp Asp Thr Ala Met Gly

325 330 335

Phe Arg Val Leu Arg Ala His Gly Tyr Asp Val Thr Pro Asp Val Phe

340 345 350

Arg Gln Phe Glu Lys Asp Gly Lys Phe Val Cys Phe Ala Gly Gln Ser

355 360 365

Thr Gln Ala Val Thr Gly Met Phe Asn Val Tyr Arg Ala Ser Gln Met

370 375 380

Leu Phe Pro Gly Glu Arg Ile Leu Glu Asp Ala Lys Lys Phe Ser Tyr

385 390 395 400

Asn Tyr Leu Lys Glu Lys Gln Ser Thr Asn Glu Leu Leu Asp Lys Trp

405 410 415

Ile Ile Ala Lys Asp Leu Pro Gly Glu Val Gly Tyr Ala Leu Asp Ile

420 425 430

Pro Trp Tyr Ala Ser Leu Pro Arg Leu Glu Thr Arg Tyr Tyr Leu Glu

435 440 445

Gln Tyr Gly Gly Glu Asp Asp Val Trp Ile Gly Lys Thr Leu Tyr Arg

450 455 460

Met Gly Tyr Val Ser Asn Asn Thr Tyr Leu Glu Met Ala Lys Leu Asp

465 470 475 480

Tyr Asn Asn Tyr Val Ala Val Leu Gln Leu Glu Trp Tyr Thr Ile Gln

485 490 495

Gln Trp Tyr Val Asp Ile Gly Ile Glu Lys Phe Glu Ser Asp Asn Ile

500 505 510

Lys Ser Val Leu Val Ser Tyr Tyr Leu Ala Ala Ala Ser Ile Phe Glu

515 520 525

Pro Glu Arg Ser Lys Glu Arg Ile Ala Trp Ala Lys Thr Thr Ile Leu

530 535 540

Val Asp Lys Ile Thr Ser Ile Phe Asp Ser Ser Gln Ser Ser Lys Glu

545 550 555 560

Asp Ile Thr Ala Phe Ile Asp Lys Phe Arg Asn Lys Ser Ser Ser Lys

565 570 575

Lys His Ser Ile Asn Gly Glu Pro Trp His Glu Val Met Val Ala Leu

580 585 590

Lys Lys Thr Leu His Gly Phe Ala Leu Asp Ala Leu Met Thr His Ser

595 600 605

Gln Asp Ile His Pro Gln Leu His Gln Ala Trp Glu Met Trp Leu Thr

610 615 620

Lys Leu Gln Asp Gly Val Asp Val Thr Ala Glu Leu Met Val Gln Met

625 630 635 640

Ile Asn Met Thr Ala Gly Arg Trp Val Ser Lys Glu Leu Leu Thr His

645 650 655

Pro Gln Tyr Gln Arg Leu Ser Thr Val Thr Asn Ser Val Cys His Asp

660 665 670

Ile Thr Lys Leu His Asn Phe Lys Glu Asn Ser Thr Thr Val Asp Ser

675 680 685

Lys Val Gln Glu Leu Val Gln Leu Val Phe Ser Asp Thr Pro Asp Asp

690 695 700

Leu Asp Gln Asp Met Lys Gln Thr Phe Leu Thr Val Met Lys Thr Phe

705 710 715 720

Tyr Tyr Lys Ala Trp Cys Asp Pro Asn Thr Ile Asn Asp His Ile Ser

725 730 735

Lys Val Phe Glu Ile Val Ile

740

<210> 63

<211> 2352

<212> DNA

<213> Stevia rebaudiana

<220>

<221> CDS

<222> (1)..(2352)

<400> 63

atg aac ttg tct cta tgt att gct tct cca tta ttg acc aaa tct aac 48

Met Asn Leu Ser Leu Cys Ile Ala Ser Pro Leu Leu Thr Lys Ser Asn

1 5 10 15

aga cca gcc gct cta tcc gct att cac act gcc tcc act tct cac ggt 96

Arg Pro Ala Ala Leu Ser Ala Ile His Thr Ala Ser Thr Ser His Gly

20 25 30

ggt caa acc aac cca acc aac ttg att att gac acc acc aag gaa aga 144

Gly Gln Thr Asn Pro Thr Asn Leu Ile Ile Asp Thr Thr Lys Glu Arg

35 40 45

atc caa aag caa ttc aag aat gtt gaa atc tcc gtt tcc tcc tac gac 192

Ile Gln Lys Gln Phe Lys Asn Val Glu Ile Ser Val Ser Ser Tyr Asp

50 55 60

act gct tgg gtt gcc atg gtt cca tct cca aac tcc cca aag tct cca 240

Thr Ala Trp Val Ala Met Val Pro Ser Pro Asn Ser Pro Lys Ser Pro

65 70 75 80

tgt ttc cca gaa tgt ttg aac tgg tta atc aac aac caa ttg aac gat 288

Cys Phe Pro Glu Cys Leu Asn Trp Leu Ile Asn Asn Gln Leu Asn Asp

85 90 95

ggt tcc tgg ggt tta gtc aat cac acc cac aac cac aat cac cca ttg 336

Gly Ser Trp Gly Leu Val Asn His Thr His Asn His Asn His Pro Leu

100 105 110

ttg aag gac tct cta tcc tcc act ttg gct tgt atc gtt gct ttg aag 384

Leu Lys Asp Ser Leu Ser Ser Thr Leu Ala Cys Ile Val Ala Leu Lys

115 120 125

aga tgg aac gtt ggt gaa gac caa atc aac aag ggt ttg tcc ttt att 432

Arg Trp Asn Val Gly Glu Asp Gln Ile Asn Lys Gly Leu Ser Phe Ile

130 135 140

gaa tcc aac ttg gct tct gct act gaa aag tcc caa cca tct cct atc 480

Glu Ser Asn Leu Ala Ser Ala Thr Glu Lys Ser Gln Pro Ser Pro Ile

145 150 155 160

ggt ttt gac atc att ttc cca ggt tta ttg gaa tac gct aag aac ttg 528

Gly Phe Asp Ile Ile Phe Pro Gly Leu Leu Glu Tyr Ala Lys Asn Leu

165 170 175

gac atc aac tta tta tct aag caa acc gat ttc tcc ttg atg ttg cac 576

Asp Ile Asn Leu Leu Ser Lys Gln Thr Asp Phe Ser Leu Met Leu His

180 185 190

aag aga gaa ttg gaa caa aag aga tgt cac tcc aac gaa atg gac ggt 624

Lys Arg Glu Leu Glu Gln Lys Arg Cys His Ser Asn Glu Met Asp Gly

195 200 205

tac ttg gct tac att tct gaa ggt ttg ggt aac ttg tac gac tgg aac 672

Tyr Leu Ala Tyr Ile Ser Glu Gly Leu Gly Asn Leu Tyr Asp Trp Asn

210 215 220

atg gtc aag aaa tac caa atg aag aac ggt tcc gtt ttc aac tct cca 720

Met Val Lys Lys Tyr Gln Met Lys Asn Gly Ser Val Phe Asn Ser Pro

225 230 235 240

tct gct acc gct gct gct ttc atc aac cat caa aac cca ggt tgt ttg 768

Ser Ala Thr Ala Ala Ala Phe Ile Asn His Gln Asn Pro Gly Cys Leu

245 250 255

aac tac ttg aac tct ttg ttg gac aaa ttc ggt aac gct gtt cca act 816

Asn Tyr Leu Asn Ser Leu Leu Asp Lys Phe Gly Asn Ala Val Pro Thr

260 265 270

gtc tac cca cac gat ttg ttt atc aga tta tcc atg gtt gac acc att 864

Val Tyr Pro His Asp Leu Phe Ile Arg Leu Ser Met Val Asp Thr Ile

275 280 285

gaa cgt ttg ggt att tct cat cac ttc aga gtc gaa atc aag aac gtt 912

Glu Arg Leu Gly Ile Ser His His Phe Arg Val Glu Ile Lys Asn Val

290 295 300

ttg gat gaa act tac aga tgt tgg gtt gaa aga gat gaa caa atc ttc 960

Leu Asp Glu Thr Tyr Arg Cys Trp Val Glu Arg Asp Glu Gln Ile Phe

305 310 315 320

atg gat gtc gtc act tgt gcc ttg gcc ttc aga tta ttg aga att aac 1008

Met Asp Val Val Thr Cys Ala Leu Ala Phe Arg Leu Leu Arg Ile Asn

325 330 335

ggt tac gaa gtt tct cca gac cca ttg gct gaa atc act aac gaa ttg 1056

Gly Tyr Glu Val Ser Pro Asp Pro Leu Ala Glu Ile Thr Asn Glu Leu

340 345 350

gct ttg aag gac gaa tac gcc gct ttg gaa act tac cat gcc tct cac 1104

Ala Leu Lys Asp Glu Tyr Ala Ala Leu Glu Thr Tyr His Ala Ser His

355 360 365

atc tta tac caa gaa gac ttg tcc tct ggt aag caa atc ttg aag tct 1152

Ile Leu Tyr Gln Glu Asp Leu Ser Ser Gly Lys Gln Ile Leu Lys Ser

370 375 380

gct gac ttc ttg aag gaa att atc tct act gat tct aac aga ttg tcc 1200

Ala Asp Phe Leu Lys Glu Ile Ile Ser Thr Asp Ser Asn Arg Leu Ser

385 390 395 400

aag ttg att cac aag gaa gtt gaa aac gcc ttg aaa ttc cca atc aac 1248

Lys Leu Ile His Lys Glu Val Glu Asn Ala Leu Lys Phe Pro Ile Asn

405 410 415

act ggt ttg gaa aga att aac acc aga aga aac atc caa tta tac aac 1296

Thr Gly Leu Glu Arg Ile Asn Thr Arg Arg Asn Ile Gln Leu Tyr Asn

420 425 430

gtt gac aac act aga atc ttg aag act act tat cac tct tcc aac atc 1344

Val Asp Asn Thr Arg Ile Leu Lys Thr Thr Tyr His Ser Ser Asn Ile

435 440 445

tcc aac act gac tac ttg aga ttg gct gtc gaa gat ttc tac acc tgt 1392

Ser Asn Thr Asp Tyr Leu Arg Leu Ala Val Glu Asp Phe Tyr Thr Cys

450 455 460

caa tct att tac aga gaa gaa ttg aag ggt ttg gaa aga tgg gtt gtc 1440

Gln Ser Ile Tyr Arg Glu Glu Leu Lys Gly Leu Glu Arg Trp Val Val

465 470 475 480

gaa aac aaa ttg gac caa ttg aaa ttt gct aga caa aag acc gcc tac 1488

Glu Asn Lys Leu Asp Gln Leu Lys Phe Ala Arg Gln Lys Thr Ala Tyr

485 490 495

tgt tac ttc tcc gtt gct gcc act ttg tcc tct cca gaa tta tct gac 1536

Cys Tyr Phe Ser Val Ala Ala Thr Leu Ser Ser Pro Glu Leu Ser Asp

500 505 510

gcc aga atc tcc tgg gct aag aat ggt atc ttg acc acc gtt gtc gat 1584

Ala Arg Ile Ser Trp Ala Lys Asn Gly Ile Leu Thr Thr Val Val Asp

515 520 525

gac ttc ttc gat att ggt ggt acc att gac gaa ttg acc aac ttg att 1632

Asp Phe Phe Asp Ile Gly Gly Thr Ile Asp Glu Leu Thr Asn Leu Ile

530 535 540

caa tgt gtt gaa aag tgg aac gtc gat gtc gat aag gac tgt tgt tct 1680

Gln Cys Val Glu Lys Trp Asn Val Asp Val Asp Lys Asp Cys Cys Ser

545 550 555 560

gaa cac gtc aga atc tta ttc ttg gct ttg aaa gat gct atc tgt tgg 1728

Glu His Val Arg Ile Leu Phe Leu Ala Leu Lys Asp Ala Ile Cys Trp

565 570 575

atc ggt gac gaa gct ttc aaa tgg caa gct cgt gac gtt acc tct cac 1776

Ile Gly Asp Glu Ala Phe Lys Trp Gln Ala Arg Asp Val Thr Ser His

580 585 590

gtc atc caa acc tgg ttg gaa ttg atg aac tct atg ttg aga gaa gcc 1824

Val Ile Gln Thr Trp Leu Glu Leu Met Asn Ser Met Leu Arg Glu Ala

595 600 605

atc tgg acc cgt gat gct tac gtc cca act ttg aac gaa tac atg gaa 1872

Ile Trp Thr Arg Asp Ala Tyr Val Pro Thr Leu Asn Glu Tyr Met Glu

610 615 620

aat gct tac gtt tct ttc gct ttg ggt cca att gtc aag cct gct att 1920

Asn Ala Tyr Val Ser Phe Ala Leu Gly Pro Ile Val Lys Pro Ala Ile

625 630 635 640

tac ttc gtt ggt cca aag ttg tcc gaa gaa att gtt gaa tct tct gaa 1968

Tyr Phe Val Gly Pro Lys Leu Ser Glu Glu Ile Val Glu Ser Ser Glu

645 650 655

tac cac aac ttg ttc aaa ttg atg tct act caa ggt cgt ttg ttg aac 2016

Tyr His Asn Leu Phe Lys Leu Met Ser Thr Gln Gly Arg Leu Leu Asn

660 665 670

gat atc cac tct ttc aag cgt gaa ttc aag gaa ggt aag ttg aat gct 2064

Asp Ile His Ser Phe Lys Arg Glu Phe Lys Glu Gly Lys Leu Asn Ala

675 680 685

gtt gct ttg cat ttg tct aac ggt gaa tct ggt aag gtc gaa gaa gaa 2112

Val Ala Leu His Leu Ser Asn Gly Glu Ser Gly Lys Val Glu Glu Glu

690 695 700

gtt gtc gaa gaa atg atg atg atg atc aag aac aag aga aag gaa ttg 2160

Val Val Glu Glu Met Met Met Met Ile Lys Asn Lys Arg Lys Glu Leu

705 710 715 720

atg aag ttg atc ttt gaa gaa aac ggt tct att gtc cca aga gct tgt 2208

Met Lys Leu Ile Phe Glu Glu Asn Gly Ser Ile Val Pro Arg Ala Cys

725 730 735

aag gat gct ttc tgg aac atg tgt cac gtc ttg aac ttc ttc tac gct 2256

Lys Asp Ala Phe Trp Asn Met Cys His Val Leu Asn Phe Phe Tyr Ala

740 745 750

aac gat gac ggt ttc act ggt aac acc atc tta gac acc gtc aag gac 2304

Asn Asp Asp Gly Phe Thr Gly Asn Thr Ile Leu Asp Thr Val Lys Asp

755 760 765

atc att tac aac cca tta gtc ttg gtt aac gaa aac gaa gaa caa aga 2352

Ile Ile Tyr Asn Pro Leu Val Leu Val Asn Glu Asn Glu Glu Gln Arg

770 775 780

<210> 64

<211> 784

<212> PRT

<213> Stevia rebaudiana

<400> 64

Met Asn Leu Ser Leu Cys Ile Ala Ser Pro Leu Leu Thr Lys Ser Asn

1 5 10 15

Arg Pro Ala Ala Leu Ser Ala Ile His Thr Ala Ser Thr Ser His Gly

20 25 30

Gly Gln Thr Asn Pro Thr Asn Leu Ile Ile Asp Thr Thr Lys Glu Arg

35 40 45

Ile Gln Lys Gln Phe Lys Asn Val Glu Ile Ser Val Ser Ser Tyr Asp

50 55 60

Thr Ala Trp Val Ala Met Val Pro Ser Pro Asn Ser Pro Lys Ser Pro

65 70 75 80

Cys Phe Pro Glu Cys Leu Asn Trp Leu Ile Asn Asn Gln Leu Asn Asp

85 90 95

Gly Ser Trp Gly Leu Val Asn His Thr His Asn His Asn His Pro Leu

100 105 110

Leu Lys Asp Ser Leu Ser Ser Thr Leu Ala Cys Ile Val Ala Leu Lys

115 120 125

Arg Trp Asn Val Gly Glu Asp Gln Ile Asn Lys Gly Leu Ser Phe Ile

130 135 140

Glu Ser Asn Leu Ala Ser Ala Thr Glu Lys Ser Gln Pro Ser Pro Ile

145 150 155 160

Gly Phe Asp Ile Ile Phe Pro Gly Leu Leu Glu Tyr Ala Lys Asn Leu

165 170 175

Asp Ile Asn Leu Leu Ser Lys Gln Thr Asp Phe Ser Leu Met Leu His

180 185 190

Lys Arg Glu Leu Glu Gln Lys Arg Cys His Ser Asn Glu Met Asp Gly

195 200 205

Tyr Leu Ala Tyr Ile Ser Glu Gly Leu Gly Asn Leu Tyr Asp Trp Asn

210 215 220

Met Val Lys Lys Tyr Gln Met Lys Asn Gly Ser Val Phe Asn Ser Pro

225 230 235 240

Ser Ala Thr Ala Ala Ala Phe Ile Asn His Gln Asn Pro Gly Cys Leu

245 250 255

Asn Tyr Leu Asn Ser Leu Leu Asp Lys Phe Gly Asn Ala Val Pro Thr

260 265 270

Val Tyr Pro His Asp Leu Phe Ile Arg Leu Ser Met Val Asp Thr Ile

275 280 285

Glu Arg Leu Gly Ile Ser His His Phe Arg Val Glu Ile Lys Asn Val

290 295 300

Leu Asp Glu Thr Tyr Arg Cys Trp Val Glu Arg Asp Glu Gln Ile Phe

305 310 315 320

Met Asp Val Val Thr Cys Ala Leu Ala Phe Arg Leu Leu Arg Ile Asn

325 330 335

Gly Tyr Glu Val Ser Pro Asp Pro Leu Ala Glu Ile Thr Asn Glu Leu

340 345 350

Ala Leu Lys Asp Glu Tyr Ala Ala Leu Glu Thr Tyr His Ala Ser His

355 360 365

Ile Leu Tyr Gln Glu Asp Leu Ser Ser Gly Lys Gln Ile Leu Lys Ser

370 375 380

Ala Asp Phe Leu Lys Glu Ile Ile Ser Thr Asp Ser Asn Arg Leu Ser

385 390 395 400

Lys Leu Ile His Lys Glu Val Glu Asn Ala Leu Lys Phe Pro Ile Asn

405 410 415

Thr Gly Leu Glu Arg Ile Asn Thr Arg Arg Asn Ile Gln Leu Tyr Asn

420 425 430

Val Asp Asn Thr Arg Ile Leu Lys Thr Thr Tyr His Ser Ser Asn Ile

435 440 445

Ser Asn Thr Asp Tyr Leu Arg Leu Ala Val Glu Asp Phe Tyr Thr Cys

450 455 460

Gln Ser Ile Tyr Arg Glu Glu Leu Lys Gly Leu Glu Arg Trp Val Val

465 470 475 480

Glu Asn Lys Leu Asp Gln Leu Lys Phe Ala Arg Gln Lys Thr Ala Tyr

485 490 495

Cys Tyr Phe Ser Val Ala Ala Thr Leu Ser Ser Pro Glu Leu Ser Asp

500 505 510

Ala Arg Ile Ser Trp Ala Lys Asn Gly Ile Leu Thr Thr Val Val Asp

515 520 525

Asp Phe Phe Asp Ile Gly Gly Thr Ile Asp Glu Leu Thr Asn Leu Ile

530 535 540

Gln Cys Val Glu Lys Trp Asn Val Asp Val Asp Lys Asp Cys Cys Ser

545 550 555 560

Glu His Val Arg Ile Leu Phe Leu Ala Leu Lys Asp Ala Ile Cys Trp

565 570 575

Ile Gly Asp Glu Ala Phe Lys Trp Gln Ala Arg Asp Val Thr Ser His

580 585 590

Val Ile Gln Thr Trp Leu Glu Leu Met Asn Ser Met Leu Arg Glu Ala

595 600 605

Ile Trp Thr Arg Asp Ala Tyr Val Pro Thr Leu Asn Glu Tyr Met Glu

610 615 620

Asn Ala Tyr Val Ser Phe Ala Leu Gly Pro Ile Val Lys Pro Ala Ile

625 630 635 640

Tyr Phe Val Gly Pro Lys Leu Ser Glu Glu Ile Val Glu Ser Ser Glu

645 650 655

Tyr His Asn Leu Phe Lys Leu Met Ser Thr Gln Gly Arg Leu Leu Asn

660 665 670

Asp Ile His Ser Phe Lys Arg Glu Phe Lys Glu Gly Lys Leu Asn Ala

675 680 685

Val Ala Leu His Leu Ser Asn Gly Glu Ser Gly Lys Val Glu Glu Glu

690 695 700

Val Val Glu Glu Met Met Met Met Ile Lys Asn Lys Arg Lys Glu Leu

705 710 715 720

Met Lys Leu Ile Phe Glu Glu Asn Gly Ser Ile Val Pro Arg Ala Cys

725 730 735

Lys Asp Ala Phe Trp Asn Met Cys His Val Leu Asn Phe Phe Tyr Ala

740 745 750

Asn Asp Asp Gly Phe Thr Gly Asn Thr Ile Leu Asp Thr Val Lys Asp

755 760 765

Ile Ile Tyr Asn Pro Leu Val Leu Val Asn Glu Asn Glu Glu Gln Arg

770 775 780

<210> 65

<211> 2271

<212> DNA

<213> Stevia rebaudiana

<220>

<221> CDS

<222> (1)..(2271)

<400> 65

atg act tct cac ggt ggt caa acc aac cca acc aac ttg att att gac 48

Met Thr Ser His Gly Gly Gln Thr Asn Pro Thr Asn Leu Ile Ile Asp

1 5 10 15

acc acc aag gaa aga atc caa aag caa ttc aag aat gtt gaa atc tcc 96

Thr Thr Lys Glu Arg Ile Gln Lys Gln Phe Lys Asn Val Glu Ile Ser

20 25 30

gtt tcc tcc tac gac act gct tgg gtt gcc atg gtt cca tct cca aac 144

Val Ser Ser Tyr Asp Thr Ala Trp Val Ala Met Val Pro Ser Pro Asn

35 40 45

tcc cca aag tct cca tgt ttc cca gaa tgt ttg aac tgg tta atc aac 192

Ser Pro Lys Ser Pro Cys Phe Pro Glu Cys Leu Asn Trp Leu Ile Asn

50 55 60

aac caa ttg aac gat ggt tcc tgg ggt tta gtc aat cac acc cac aac 240

Asn Gln Leu Asn Asp Gly Ser Trp Gly Leu Val Asn His Thr His Asn

65 70 75 80

cac aat cac cca ttg ttg aag gac tct cta tcc tcc act ttg gct tgt 288

His Asn His Pro Leu Leu Lys Asp Ser Leu Ser Ser Thr Leu Ala Cys

85 90 95

atc gtt gct ttg aag aga tgg aac gtt ggt gaa gac caa atc aac aag 336

Ile Val Ala Leu Lys Arg Trp Asn Val Gly Glu Asp Gln Ile Asn Lys

100 105 110

ggt ttg tcc ttt att gaa tcc aac ttg gct tct gct act gaa aag tcc 384

Gly Leu Ser Phe Ile Glu Ser Asn Leu Ala Ser Ala Thr Glu Lys Ser

115 120 125

caa cca tct cct atc ggt ttt gac atc att ttc cca ggt tta ttg gaa 432

Gln Pro Ser Pro Ile Gly Phe Asp Ile Ile Phe Pro Gly Leu Leu Glu

130 135 140

tac gct aag aac ttg gac atc aac tta tta tct aag caa acc gat ttc 480

Tyr Ala Lys Asn Leu Asp Ile Asn Leu Leu Ser Lys Gln Thr Asp Phe

145 150 155 160

tcc ttg atg ttg cac aag aga gaa ttg gaa caa aag aga tgt cac tcc 528

Ser Leu Met Leu His Lys Arg Glu Leu Glu Gln Lys Arg Cys His Ser

165 170 175

aac gaa atg gac ggt tac ttg gct tac att tct gaa ggt ttg ggt aac 576

Asn Glu Met Asp Gly Tyr Leu Ala Tyr Ile Ser Glu Gly Leu Gly Asn

180 185 190

ttg tac gac tgg aac atg gtc aag aaa tac caa atg aag aac ggt tcc 624

Leu Tyr Asp Trp Asn Met Val Lys Lys Tyr Gln Met Lys Asn Gly Ser

195 200 205

gtt ttc aac tct cca tct gct acc gct gct gct ttc atc aac cat caa 672

Val Phe Asn Ser Pro Ser Ala Thr Ala Ala Ala Phe Ile Asn His Gln

210 215 220

aac cca ggt tgt ttg aac tac ttg aac tct ttg ttg gac aaa ttc ggt 720

Asn Pro Gly Cys Leu Asn Tyr Leu Asn Ser Leu Leu Asp Lys Phe Gly

225 230 235 240

aac gct gtt cca act gtc tac cca cac gat ttg ttt atc aga tta tcc 768

Asn Ala Val Pro Thr Val Tyr Pro His Asp Leu Phe Ile Arg Leu Ser

245 250 255

atg gtt gac acc att gaa cgt ttg ggt att tct cat cac ttc aga gtc 816

Met Val Asp Thr Ile Glu Arg Leu Gly Ile Ser His His Phe Arg Val

260 265 270

gaa atc aag aac gtt ttg gat gaa act tac aga tgt tgg gtt gaa aga 864

Glu Ile Lys Asn Val Leu Asp Glu Thr Tyr Arg Cys Trp Val Glu Arg

275 280 285

gat gaa caa atc ttc atg gat gtc gtc act tgt gcc ttg gcc ttc aga 912

Asp Glu Gln Ile Phe Met Asp Val Val Thr Cys Ala Leu Ala Phe Arg

290 295 300

tta ttg aga att aac ggt tac gaa gtt tct cca gac cca ttg gct gaa 960

Leu Leu Arg Ile Asn Gly Tyr Glu Val Ser Pro Asp Pro Leu Ala Glu

305 310 315 320

atc act aac gaa ttg gct ttg aag gac gaa tac gcc gct ttg gaa act 1008

Ile Thr Asn Glu Leu Ala Leu Lys Asp Glu Tyr Ala Ala Leu Glu Thr

325 330 335

tac cat gcc tct cac atc tta tac caa gaa gac ttg tcc tct ggt aag 1056

Tyr His Ala Ser His Ile Leu Tyr Gln Glu Asp Leu Ser Ser Gly Lys

340 345 350

caa atc ttg aag tct gct gac ttc ttg aag gaa att atc tct act gat 1104

Gln Ile Leu Lys Ser Ala Asp Phe Leu Lys Glu Ile Ile Ser Thr Asp

355 360 365

tct aac aga ttg tcc aag ttg att cac aag gaa gtt gaa aac gcc ttg 1152

Ser Asn Arg Leu Ser Lys Leu Ile His Lys Glu Val Glu Asn Ala Leu

370 375 380

aaa ttc cca atc aac act ggt ttg gaa aga att aac acc aga aga aac 1200

Lys Phe Pro Ile Asn Thr Gly Leu Glu Arg Ile Asn Thr Arg Arg Asn

385 390 395 400

atc caa tta tac aac gtt gac aac act aga atc ttg aag act act tat 1248

Ile Gln Leu Tyr Asn Val Asp Asn Thr Arg Ile Leu Lys Thr Thr Tyr

405 410 415

cac tct tcc aac atc tcc aac act gac tac ttg aga ttg gct gtc gaa 1296

His Ser Ser Asn Ile Ser Asn Thr Asp Tyr Leu Arg Leu Ala Val Glu

420 425 430

gat ttc tac acc tgt caa tct att tac aga gaa gaa ttg aag ggt ttg 1344

Asp Phe Tyr Thr Cys Gln Ser Ile Tyr Arg Glu Glu Leu Lys Gly Leu

435 440 445

gaa aga tgg gtt gtc gaa aac aaa ttg gac caa ttg aaa ttt gct aga 1392

Glu Arg Trp Val Val Glu Asn Lys Leu Asp Gln Leu Lys Phe Ala Arg

450 455 460

caa aag acc gcc tac tgt tac ttc tcc gtt gct gcc act ttg tcc tct 1440

Gln Lys Thr Ala Tyr Cys Tyr Phe Ser Val Ala Ala Thr Leu Ser Ser

465 470 475 480

cca gaa tta tct gac gcc aga atc tcc tgg gct aag aat ggt atc ttg 1488

Pro Glu Leu Ser Asp Ala Arg Ile Ser Trp Ala Lys Asn Gly Ile Leu

485 490 495

acc acc gtt gtc gat gac ttc ttc gat att ggt ggt acc att gac gaa 1536

Thr Thr Val Val Asp Asp Phe Phe Asp Ile Gly Gly Thr Ile Asp Glu

500 505 510

ttg acc aac ttg att caa tgt gtt gaa aag tgg aac gtc gat gtc gat 1584

Leu Thr Asn Leu Ile Gln Cys Val Glu Lys Trp Asn Val Asp Val Asp

515 520 525

aag gac tgt tgt tct gaa cac gtc aga atc tta ttc ttg gct ttg aaa 1632

Lys Asp Cys Cys Ser Glu His Val Arg Ile Leu Phe Leu Ala Leu Lys

530 535 540

gat gct atc tgt tgg atc ggt gac gaa gct ttc aaa tgg caa gct cgt 1680

Asp Ala Ile Cys Trp Ile Gly Asp Glu Ala Phe Lys Trp Gln Ala Arg

545 550 555 560

gac gtt acc tct cac gtc atc caa acc tgg ttg gaa ttg atg aac tct 1728

Asp Val Thr Ser His Val Ile Gln Thr Trp Leu Glu Leu Met Asn Ser

565 570 575

atg ttg aga gaa gcc atc tgg acc cgt gat gct tac gtc cca act ttg 1776

Met Leu Arg Glu Ala Ile Trp Thr Arg Asp Ala Tyr Val Pro Thr Leu

580 585 590

aac gaa tac atg gaa aat gct tac gtt tct ttc gct ttg ggt cca att 1824

Asn Glu Tyr Met Glu Asn Ala Tyr Val Ser Phe Ala Leu Gly Pro Ile

595 600 605

gtc aag cct gct att tac ttc gtt ggt cca aag ttg tcc gaa gaa att 1872

Val Lys Pro Ala Ile Tyr Phe Val Gly Pro Lys Leu Ser Glu Glu Ile

610 615 620

gtt gaa tct tct gaa tac cac aac ttg ttc aaa ttg atg tct act caa 1920

Val Glu Ser Ser Glu Tyr His Asn Leu Phe Lys Leu Met Ser Thr Gln

625 630 635 640

ggt cgt ttg ttg aac gat atc cac tct ttc aag cgt gaa ttc aag gaa 1968

Gly Arg Leu Leu Asn Asp Ile His Ser Phe Lys Arg Glu Phe Lys Glu

645 650 655

ggt aag ttg aat gct gtt gct ttg cat ttg tct aac ggt gaa tct ggt 2016

Gly Lys Leu Asn Ala Val Ala Leu His Leu Ser Asn Gly Glu Ser Gly

660 665 670

aag gtc gaa gaa gaa gtt gtc gaa gaa atg atg atg atg atc aag aac 2064

Lys Val Glu Glu Glu Val Val Glu Glu Met Met Met Met Ile Lys Asn

675 680 685

aag aga aag gaa ttg atg aag ttg atc ttt gaa gaa aac ggt tct att 2112

Lys Arg Lys Glu Leu Met Lys Leu Ile Phe Glu Glu Asn Gly Ser Ile

690 695 700

gtc cca aga gct tgt aag gat gct ttc tgg aac atg tgt cac gtc ttg 2160

Val Pro Arg Ala Cys Lys Asp Ala Phe Trp Asn Met Cys His Val Leu

705 710 715 720

aac ttc ttc tac gct aac gat gac ggt ttc act ggt aac acc atc tta 2208

Asn Phe Phe Tyr Ala Asn Asp Asp Gly Phe Thr Gly Asn Thr Ile Leu

725 730 735

gac acc gtc aag gac atc att tac aac cca tta gtc ttg gtt aac gaa 2256

Asp Thr Val Lys Asp Ile Ile Tyr Asn Pro Leu Val Leu Val Asn Glu

740 745 750

aac gaa gaa caa aga 2271

Asn Glu Glu Gln Arg

755

<210> 66

<211> 757

<212> PRT

<213> Stevia rebaudiana

<400> 66

Met Thr Ser His Gly Gly Gln Thr Asn Pro Thr Asn Leu Ile Ile Asp

1 5 10 15

Thr Thr Lys Glu Arg Ile Gln Lys Gln Phe Lys Asn Val Glu Ile Ser

20 25 30

Val Ser Ser Tyr Asp Thr Ala Trp Val Ala Met Val Pro Ser Pro Asn

35 40 45

Ser Pro Lys Ser Pro Cys Phe Pro Glu Cys Leu Asn Trp Leu Ile Asn

50 55 60

Asn Gln Leu Asn Asp Gly Ser Trp Gly Leu Val Asn His Thr His Asn

65 70 75 80

His Asn His Pro Leu Leu Lys Asp Ser Leu Ser Ser Thr Leu Ala Cys

85 90 95

Ile Val Ala Leu Lys Arg Trp Asn Val Gly Glu Asp Gln Ile Asn Lys

100 105 110

Gly Leu Ser Phe Ile Glu Ser Asn Leu Ala Ser Ala Thr Glu Lys Ser

115 120 125

Gln Pro Ser Pro Ile Gly Phe Asp Ile Ile Phe Pro Gly Leu Leu Glu

130 135 140

Tyr Ala Lys Asn Leu Asp Ile Asn Leu Leu Ser Lys Gln Thr Asp Phe

145 150 155 160

Ser Leu Met Leu His Lys Arg Glu Leu Glu Gln Lys Arg Cys His Ser

165 170 175

Asn Glu Met Asp Gly Tyr Leu Ala Tyr Ile Ser Glu Gly Leu Gly Asn

180 185 190

Leu Tyr Asp Trp Asn Met Val Lys Lys Tyr Gln Met Lys Asn Gly Ser

195 200 205

Val Phe Asn Ser Pro Ser Ala Thr Ala Ala Ala Phe Ile Asn His Gln

210 215 220

Asn Pro Gly Cys Leu Asn Tyr Leu Asn Ser Leu Leu Asp Lys Phe Gly

225 230 235 240

Asn Ala Val Pro Thr Val Tyr Pro His Asp Leu Phe Ile Arg Leu Ser

245 250 255

Met Val Asp Thr Ile Glu Arg Leu Gly Ile Ser His His Phe Arg Val

260 265 270

Glu Ile Lys Asn Val Leu Asp Glu Thr Tyr Arg Cys Trp Val Glu Arg

275 280 285

Asp Glu Gln Ile Phe Met Asp Val Val Thr Cys Ala Leu Ala Phe Arg

290 295 300

Leu Leu Arg Ile Asn Gly Tyr Glu Val Ser Pro Asp Pro Leu Ala Glu

305 310 315 320

Ile Thr Asn Glu Leu Ala Leu Lys Asp Glu Tyr Ala Ala Leu Glu Thr

325 330 335

Tyr His Ala Ser His Ile Leu Tyr Gln Glu Asp Leu Ser Ser Gly Lys

340 345 350

Gln Ile Leu Lys Ser Ala Asp Phe Leu Lys Glu Ile Ile Ser Thr Asp

355 360 365

Ser Asn Arg Leu Ser Lys Leu Ile His Lys Glu Val Glu Asn Ala Leu

370 375 380

Lys Phe Pro Ile Asn Thr Gly Leu Glu Arg Ile Asn Thr Arg Arg Asn

385 390 395 400

Ile Gln Leu Tyr Asn Val Asp Asn Thr Arg Ile Leu Lys Thr Thr Tyr

405 410 415

His Ser Ser Asn Ile Ser Asn Thr Asp Tyr Leu Arg Leu Ala Val Glu

420 425 430

Asp Phe Tyr Thr Cys Gln Ser Ile Tyr Arg Glu Glu Leu Lys Gly Leu

435 440 445

Glu Arg Trp Val Val Glu Asn Lys Leu Asp Gln Leu Lys Phe Ala Arg

450 455 460

Gln Lys Thr Ala Tyr Cys Tyr Phe Ser Val Ala Ala Thr Leu Ser Ser

465 470 475 480

Pro Glu Leu Ser Asp Ala Arg Ile Ser Trp Ala Lys Asn Gly Ile Leu

485 490 495

Thr Thr Val Val Asp Asp Phe Phe Asp Ile Gly Gly Thr Ile Asp Glu

500 505 510

Leu Thr Asn Leu Ile Gln Cys Val Glu Lys Trp Asn Val Asp Val Asp

515 520 525

Lys Asp Cys Cys Ser Glu His Val Arg Ile Leu Phe Leu Ala Leu Lys

530 535 540

Asp Ala Ile Cys Trp Ile Gly Asp Glu Ala Phe Lys Trp Gln Ala Arg

545 550 555 560

Asp Val Thr Ser His Val Ile Gln Thr Trp Leu Glu Leu Met Asn Ser

565 570 575

Met Leu Arg Glu Ala Ile Trp Thr Arg Asp Ala Tyr Val Pro Thr Leu

580 585 590

Asn Glu Tyr Met Glu Asn Ala Tyr Val Ser Phe Ala Leu Gly Pro Ile

595 600 605

Val Lys Pro Ala Ile Tyr Phe Val Gly Pro Lys Leu Ser Glu Glu Ile

610 615 620

Val Glu Ser Ser Glu Tyr His Asn Leu Phe Lys Leu Met Ser Thr Gln

625 630 635 640

Gly Arg Leu Leu Asn Asp Ile His Ser Phe Lys Arg Glu Phe Lys Glu

645 650 655

Gly Lys Leu Asn Ala Val Ala Leu His Leu Ser Asn Gly Glu Ser Gly

660 665 670

Lys Val Glu Glu Glu Val Val Glu Glu Met Met Met Met Ile Lys Asn

675 680 685

Lys Arg Lys Glu Leu Met Lys Leu Ile Phe Glu Glu Asn Gly Ser Ile

690 695 700

Val Pro Arg Ala Cys Lys Asp Ala Phe Trp Asn Met Cys His Val Leu

705 710 715 720

Asn Phe Phe Tyr Ala Asn Asp Asp Gly Phe Thr Gly Asn Thr Ile Leu

725 730 735

Asp Thr Val Lys Asp Ile Ile Tyr Asn Pro Leu Val Leu Val Asn Glu

740 745 750

Asn Glu Glu Gln Arg

755

<210> 67

<211> 1539

<212> DNA

<213> Stevia rebaudiana

<220>

<221> CDS

<222> (1)..(1539)

<400> 67

atg gat gct gtc act ggt ttg ttg acc gtc cca gct acc gcc atc acc 48

Met Asp Ala Val Thr Gly Leu Leu Thr Val Pro Ala Thr Ala Ile Thr

1 5 10 15

att ggt ggt act gct gtt gct ttg gct gtt gct ttg atc ttc tgg tac 96

Ile Gly Gly Thr Ala Val Ala Leu Ala Val Ala Leu Ile Phe Trp Tyr

20 25 30

ttg aaa tct tac act tct gcc aga aga tct caa tct aac cat tta cca 144

Leu Lys Ser Tyr Thr Ser Ala Arg Arg Ser Gln Ser Asn His Leu Pro

35 40 45

aga gtt cca gaa gtt cca ggt gtt cca ttg ttg ggt aac ttg ttg caa 192

Arg Val Pro Glu Val Pro Gly Val Pro Leu Leu Gly Asn Leu Leu Gln

50 55 60

ttg aaa gaa aag aag cct tac atg act ttc acc aga tgg gct gct acc 240

Leu Lys Glu Lys Lys Pro Tyr Met Thr Phe Thr Arg Trp Ala Ala Thr

65 70 75 80

tac ggt cca att tac tct atc aag acc ggt gct acc tcc atg gtt gtt 288

Tyr Gly Pro Ile Tyr Ser Ile Lys Thr Gly Ala Thr Ser Met Val Val

85 90 95

gtt tcc tcc aac gaa att gcc aag gaa gct tta gtc acc aga ttc caa 336

Val Ser Ser Asn Glu Ile Ala Lys Glu Ala Leu Val Thr Arg Phe Gln

100 105 110

tcc atc tct acc aga aac ttg tcc aag gct ttg aag gtc ttg act gct 384

Ser Ile Ser Thr Arg Asn Leu Ser Lys Ala Leu Lys Val Leu Thr Ala

115 120 125

gac aag acc atg gtt gcc atg tct gac tac gat gac tac cac aag act 432

Asp Lys Thr Met Val Ala Met Ser Asp Tyr Asp Asp Tyr His Lys Thr

130 135 140

gtc aaa cgt cac atc ttg act gct gtt ttg ggt cca aat gct caa aag 480

Val Lys Arg His Ile Leu Thr Ala Val Leu Gly Pro Asn Ala Gln Lys

145 150 155 160

aag cac aga att cac aga gat atc atg atg gac aac atc tct act caa 528

Lys His Arg Ile His Arg Asp Ile Met Met Asp Asn Ile Ser Thr Gln

165 170 175

ttg cat gaa ttt gtc aag aac aac cca gaa caa gaa gaa gtc gat ttg 576

Leu His Glu Phe Val Lys Asn Asn Pro Glu Gln Glu Glu Val Asp Leu

180 185 190

aga aag atc ttc caa tct gaa ttg ttc ggt ttg gcc atg aga caa gct 624

Arg Lys Ile Phe Gln Ser Glu Leu Phe Gly Leu Ala Met Arg Gln Ala

195 200 205

ttg ggt aaa gat gtc gaa tct tta tac gtc gaa gat ttg aag atc acc 672

Leu Gly Lys Asp Val Glu Ser Leu Tyr Val Glu Asp Leu Lys Ile Thr

210 215 220

atg aac aga gat gaa atc ttc caa gtt ttg gtt gtc gac cca atg atg 720

Met Asn Arg Asp Glu Ile Phe Gln Val Leu Val Val Asp Pro Met Met

225 230 235 240

ggt gcc att gat gtt gac tgg aga gat ttc ttc cca tac ttg aaa tgg 768

Gly Ala Ile Asp Val Asp Trp Arg Asp Phe Phe Pro Tyr Leu Lys Trp

245 250 255

gtt cca aac aag aag ttc gaa aat acc att caa caa atg tac atc cgt 816

Val Pro Asn Lys Lys Phe Glu Asn Thr Ile Gln Gln Met Tyr Ile Arg

260 265 270

cgt gaa gct gtc atg aag tct tta atc aag gaa aac aag aag aga att 864

Arg Glu Ala Val Met Lys Ser Leu Ile Lys Glu Asn Lys Lys Arg Ile

275 280 285

gct tct ggt gaa aag tta aac tcc tac att gac tat ttg ttg tcc gaa 912

Ala Ser Gly Glu Lys Leu Asn Ser Tyr Ile Asp Tyr Leu Leu Ser Glu

290 295 300

gct caa act ttg act gac caa caa tta ttg atg tct tta tgg gaa cca 960

Ala Gln Thr Leu Thr Asp Gln Gln Leu Leu Met Ser Leu Trp Glu Pro

305 310 315 320

atc att gaa tct tct gac acc acc atg gtc act act gaa tgg gct atg 1008

Ile Ile Glu Ser Ser Asp Thr Thr Met Val Thr Thr Glu Trp Ala Met

325 330 335

tac gaa ttg gcc aag aac cca aaa tta caa gac cgt ttg tac aga gat 1056

Tyr Glu Leu Ala Lys Asn Pro Lys Leu Gln Asp Arg Leu Tyr Arg Asp

340 345 350

atc aaa tcc gtc tgt ggt tcc gaa aag atc act gaa gaa cac ttg tct 1104

Ile Lys Ser Val Cys Gly Ser Glu Lys Ile Thr Glu Glu His Leu Ser

355 360 365

caa ttg cca tac atc act gct atc ttc cac gaa act ttg aga aga cac 1152

Gln Leu Pro Tyr Ile Thr Ala Ile Phe His Glu Thr Leu Arg Arg His

370 375 380

tct cca gtt cca atc att cca ttg aga cac gtc cac gaa gac act gtt 1200

Ser Pro Val Pro Ile Ile Pro Leu Arg His Val His Glu Asp Thr Val

385 390 395 400

ttg ggt ggt tac cac gtt cca gct ggt act gaa ttg gct gtc aac atc 1248

Leu Gly Gly Tyr His Val Pro Ala Gly Thr Glu Leu Ala Val Asn Ile

405 410 415

tac ggt tgt aac atg gac aag aac gtc tgg gaa aac cca gaa gaa tgg 1296

Tyr Gly Cys Asn Met Asp Lys Asn Val Trp Glu Asn Pro Glu Glu Trp

420 425 430

aac cca gaa aga ttc atg aag gaa aac gaa acc att gac ttc caa aag 1344

Asn Pro Glu Arg Phe Met Lys Glu Asn Glu Thr Ile Asp Phe Gln Lys

435 440 445

acc atg gct ttc ggt ggt ggt aag aga gtt tgt gcc ggt tcc ttg caa 1392

Thr Met Ala Phe Gly Gly Gly Lys Arg Val Cys Ala Gly Ser Leu Gln

450 455 460

gct cta ttg acc gct tcc att ggt att ggt aga atg gtt caa gaa ttt 1440

Ala Leu Leu Thr Ala Ser Ile Gly Ile Gly Arg Met Val Gln Glu Phe

465 470 475 480

gaa tgg aag ttg aag gac atg acc caa gaa gaa gtt aac acc att ggt 1488

Glu Trp Lys Leu Lys Asp Met Thr Gln Glu Glu Val Asn Thr Ile Gly

485 490 495

ttg act act caa atg tta aga cca ttg aga gcc atc atc aag cct cgc 1536

Leu Thr Thr Gln Met Leu Arg Pro Leu Arg Ala Ile Ile Lys Pro Arg

500 505 510

atc 1539

Ile

<210> 68

<211> 513

<212> PRT

<213> Stevia rebaudiana

<400> 68

Met Asp Ala Val Thr Gly Leu Leu Thr Val Pro Ala Thr Ala Ile Thr

1 5 10 15

Ile Gly Gly Thr Ala Val Ala Leu Ala Val Ala Leu Ile Phe Trp Tyr

20 25 30

Leu Lys Ser Tyr Thr Ser Ala Arg Arg Ser Gln Ser Asn His Leu Pro

35 40 45

Arg Val Pro Glu Val Pro Gly Val Pro Leu Leu Gly Asn Leu Leu Gln

50 55 60

Leu Lys Glu Lys Lys Pro Tyr Met Thr Phe Thr Arg Trp Ala Ala Thr

65 70 75 80

Tyr Gly Pro Ile Tyr Ser Ile Lys Thr Gly Ala Thr Ser Met Val Val

85 90 95

Val Ser Ser Asn Glu Ile Ala Lys Glu Ala Leu Val Thr Arg Phe Gln

100 105 110

Ser Ile Ser Thr Arg Asn Leu Ser Lys Ala Leu Lys Val Leu Thr Ala

115 120 125

Asp Lys Thr Met Val Ala Met Ser Asp Tyr Asp Asp Tyr His Lys Thr

130 135 140

Val Lys Arg His Ile Leu Thr Ala Val Leu Gly Pro Asn Ala Gln Lys

145 150 155 160

Lys His Arg Ile His Arg Asp Ile Met Met Asp Asn Ile Ser Thr Gln

165 170 175

Leu His Glu Phe Val Lys Asn Asn Pro Glu Gln Glu Glu Val Asp Leu

180 185 190

Arg Lys Ile Phe Gln Ser Glu Leu Phe Gly Leu Ala Met Arg Gln Ala

195 200 205

Leu Gly Lys Asp Val Glu Ser Leu Tyr Val Glu Asp Leu Lys Ile Thr

210 215 220

Met Asn Arg Asp Glu Ile Phe Gln Val Leu Val Val Asp Pro Met Met

225 230 235 240

Gly Ala Ile Asp Val Asp Trp Arg Asp Phe Phe Pro Tyr Leu Lys Trp

245 250 255

Val Pro Asn Lys Lys Phe Glu Asn Thr Ile Gln Gln Met Tyr Ile Arg

260 265 270

Arg Glu Ala Val Met Lys Ser Leu Ile Lys Glu Asn Lys Lys Arg Ile

275 280 285

Ala Ser Gly Glu Lys Leu Asn Ser Tyr Ile Asp Tyr Leu Leu Ser Glu

290 295 300

Ala Gln Thr Leu Thr Asp Gln Gln Leu Leu Met Ser Leu Trp Glu Pro

305 310 315 320

Ile Ile Glu Ser Ser Asp Thr Thr Met Val Thr Thr Glu Trp Ala Met

325 330 335

Tyr Glu Leu Ala Lys Asn Pro Lys Leu Gln Asp Arg Leu Tyr Arg Asp

340 345 350

Ile Lys Ser Val Cys Gly Ser Glu Lys Ile Thr Glu Glu His Leu Ser

355 360 365

Gln Leu Pro Tyr Ile Thr Ala Ile Phe His Glu Thr Leu Arg Arg His

370 375 380

Ser Pro Val Pro Ile Ile Pro Leu Arg His Val His Glu Asp Thr Val

385 390 395 400

Leu Gly Gly Tyr His Val Pro Ala Gly Thr Glu Leu Ala Val Asn Ile

405 410 415

Tyr Gly Cys Asn Met Asp Lys Asn Val Trp Glu Asn Pro Glu Glu Trp

420 425 430

Asn Pro Glu Arg Phe Met Lys Glu Asn Glu Thr Ile Asp Phe Gln Lys

435 440 445

Thr Met Ala Phe Gly Gly Gly Lys Arg Val Cys Ala Gly Ser Leu Gln

450 455 460

Ala Leu Leu Thr Ala Ser Ile Gly Ile Gly Arg Met Val Gln Glu Phe

465 470 475 480

Glu Trp Lys Leu Lys Asp Met Thr Gln Glu Glu Val Asn Thr Ile Gly

485 490 495

Leu Thr Thr Gln Met Leu Arg Pro Leu Arg Ala Ile Ile Lys Pro Arg

500 505 510

Ile

<210> 69

<211> 1566

<212> DNA

<213> Stevia rebaudiana

<220>

<221> CDS

<222> (1)..(1566)

<400> 69

atg ggt ttg ttc cca ttg gaa gac tct tac gct ttg gtt ttc gaa ggt 48

Met Gly Leu Phe Pro Leu Glu Asp Ser Tyr Ala Leu Val Phe Glu Gly

1 5 10 15

ttg gcc atc act ttg gct ttg tac tac cta ttg tct ttc atc tac aag 96

Leu Ala Ile Thr Leu Ala Leu Tyr Tyr Leu Leu Ser Phe Ile Tyr Lys

20 25 30

acc tcc aag aag acc tgt acc cca cca aag gct tct ggt gaa cac cca 144

Thr Ser Lys Lys Thr Cys Thr Pro Pro Lys Ala Ser Gly Glu His Pro

35 40 45

att acc ggt cat tta aac ttg ttg tct ggt tct tcc ggt ttg cca cac 192

Ile Thr Gly His Leu Asn Leu Leu Ser Gly Ser Ser Gly Leu Pro His

50 55 60

ttg gct ttg gct tct ttg gct gac aga tgt ggt cct atc ttc acc atc 240

Leu Ala Leu Ala Ser Leu Ala Asp Arg Cys Gly Pro Ile Phe Thr Ile

65 70 75 80

aga tta ggt atc aga aga gtt ttg gtt gtt tcc aac tgg gaa att gcc 288

Arg Leu Gly Ile Arg Arg Val Leu Val Val Ser Asn Trp Glu Ile Ala

85 90 95

aag gaa atc ttt acc acc cac gac ttg att gtc tcc aac aga cca aag 336

Lys Glu Ile Phe Thr Thr His Asp Leu Ile Val Ser Asn Arg Pro Lys

100 105 110

tac ttg gct gcc aag atc tta ggt ttc aac tac gtt tct ttc tct ttc 384

Tyr Leu Ala Ala Lys Ile Leu Gly Phe Asn Tyr Val Ser Phe Ser Phe

115 120 125

gct cca tac ggt cca tac tgg gtt ggt atc aga aag att att gct act 432

Ala Pro Tyr Gly Pro Tyr Trp Val Gly Ile Arg Lys Ile Ile Ala Thr

130 135 140

aaa ttg atg tct tct tct aga ttg caa aag ttg caa ttc gtc cgt gtc 480

Lys Leu Met Ser Ser Ser Arg Leu Gln Lys Leu Gln Phe Val Arg Val

145 150 155 160

ttt gaa ttg gaa aac tcc atg aaa tcc atc aga gaa tcc tgg aag gaa 528

Phe Glu Leu Glu Asn Ser Met Lys Ser Ile Arg Glu Ser Trp Lys Glu

165 170 175

aag aag gat gaa gaa ggt aag gtc ttg gtt gaa atg aag aag tgg ttc 576

Lys Lys Asp Glu Glu Gly Lys Val Leu Val Glu Met Lys Lys Trp Phe

180 185 190

tgg gaa ttg aat atg aac att gtc ttg aga acc gtt gct ggt aag caa 624

Trp Glu Leu Asn Met Asn Ile Val Leu Arg Thr Val Ala Gly Lys Gln

195 200 205

tac act ggt act gtt gac gat gct gat gcc aag aga att tct gaa ttg 672

Tyr Thr Gly Thr Val Asp Asp Ala Asp Ala Lys Arg Ile Ser Glu Leu

210 215 220

ttc aga gaa tgg ttc cac tac act ggt aga ttc gtt gtc ggt gat gct 720

Phe Arg Glu Trp Phe His Tyr Thr Gly Arg Phe Val Val Gly Asp Ala

225 230 235 240

ttc cca ttt cta ggt tgg ttg gat tta ggt ggt tac aag aag acc atg 768

Phe Pro Phe Leu Gly Trp Leu Asp Leu Gly Gly Tyr Lys Lys Thr Met

245 250 255

gaa ttg gtt gcc tcc aga tta gat tcc atg gtc agc aaa tgg ttg gac 816

Glu Leu Val Ala Ser Arg Leu Asp Ser Met Val Ser Lys Trp Leu Asp

260 265 270

gaa cac aga aag aag caa gct aac gat gac aag aag gaa gac atg gac 864

Glu His Arg Lys Lys Gln Ala Asn Asp Asp Lys Lys Glu Asp Met Asp

275 280 285

ttc atg gac atc atg atc tcc atg act gaa gct aac tct cca ttg gaa 912

Phe Met Asp Ile Met Ile Ser Met Thr Glu Ala Asn Ser Pro Leu Glu

290 295 300

ggt tac ggt act gac acc atc atc aag acc act tgt atg act ttg att 960

Gly Tyr Gly Thr Asp Thr Ile Ile Lys Thr Thr Cys Met Thr Leu Ile

305 310 315 320

gtt tcc ggt gtc gac acc act tct atc gtt ttg acc tgg gct ttg tct 1008

Val Ser Gly Val Asp Thr Thr Ser Ile Val Leu Thr Trp Ala Leu Ser

325 330 335

ttg ttg ttg aac aac aga gac act tta aag aag gct caa gaa gaa ttg 1056

Leu Leu Leu Asn Asn Arg Asp Thr Leu Lys Lys Ala Gln Glu Glu Leu

340 345 350

gac atg tgt gtc ggt aag ggt aga caa gtc aat gaa tct gat ttg gtc 1104

Asp Met Cys Val Gly Lys Gly Arg Gln Val Asn Glu Ser Asp Leu Val

355 360 365

aac tta atc tat tta gaa gct gtc ttg aaa gaa gct ttg aga ttg tac 1152

Asn Leu Ile Tyr Leu Glu Ala Val Leu Lys Glu Ala Leu Arg Leu Tyr

370 375 380

cca gct gct ttc ttg ggt ggt cct cgt gct ttc ttg gaa gac tgt acc 1200

Pro Ala Ala Phe Leu Gly Gly Pro Arg Ala Phe Leu Glu Asp Cys Thr

385 390 395 400

gtt gcc ggt tac aga att cca aag ggt act tgt ttg ttg atc aac atg 1248

Val Ala Gly Tyr Arg Ile Pro Lys Gly Thr Cys Leu Leu Ile Asn Met

405 410 415

tgg aaa ttg cac aga gat cca aac atc tgg tct gac cca tgt gaa ttc 1296

Trp Lys Leu His Arg Asp Pro Asn Ile Trp Ser Asp Pro Cys Glu Phe

420 425 430

aaa cca gaa aga ttc ttg act cca aac caa aag gat gtt gat gtc att 1344

Lys Pro Glu Arg Phe Leu Thr Pro Asn Gln Lys Asp Val Asp Val Ile

435 440 445

ggt atg gac ttc gaa ttg att cca ttc ggt gct ggt cgt cgt tac tgt 1392

Gly Met Asp Phe Glu Leu Ile Pro Phe Gly Ala Gly Arg Arg Tyr Cys

450 455 460

cca ggt acc aga tta gct ttg caa atg ttg cac att gtc ttg gcc act 1440

Pro Gly Thr Arg Leu Ala Leu Gln Met Leu His Ile Val Leu Ala Thr

465 470 475 480

cta tta caa aac ttt gaa atg tcc act cca aac gat gct cca gtt gac 1488

Leu Leu Gln Asn Phe Glu Met Ser Thr Pro Asn Asp Ala Pro Val Asp

485 490 495

atg act gct tcc gtt ggt atg acc aac gcc aag gct tct cca ttg gaa 1536

Met Thr Ala Ser Val Gly Met Thr Asn Ala Lys Ala Ser Pro Leu Glu

500 505 510

gtt ttg ttg tct cca aga gtc aaa tgg tct 1566

Val Leu Leu Ser Pro Arg Val Lys Trp Ser

515 520

<210> 70

<211> 522

<212> PRT

<213> Stevia rebaudiana

<400> 70

Met Gly Leu Phe Pro Leu Glu Asp Ser Tyr Ala Leu Val Phe Glu Gly

1 5 10 15

Leu Ala Ile Thr Leu Ala Leu Tyr Tyr Leu Leu Ser Phe Ile Tyr Lys

20 25 30

Thr Ser Lys Lys Thr Cys Thr Pro Pro Lys Ala Ser Gly Glu His Pro

35 40 45

Ile Thr Gly His Leu Asn Leu Leu Ser Gly Ser Ser Gly Leu Pro His

50 55 60

Leu Ala Leu Ala Ser Leu Ala Asp Arg Cys Gly Pro Ile Phe Thr Ile

65 70 75 80

Arg Leu Gly Ile Arg Arg Val Leu Val Val Ser Asn Trp Glu Ile Ala

85 90 95

Lys Glu Ile Phe Thr Thr His Asp Leu Ile Val Ser Asn Arg Pro Lys

100 105 110

Tyr Leu Ala Ala Lys Ile Leu Gly Phe Asn Tyr Val Ser Phe Ser Phe

115 120 125

Ala Pro Tyr Gly Pro Tyr Trp Val Gly Ile Arg Lys Ile Ile Ala Thr

130 135 140

Lys Leu Met Ser Ser Ser Arg Leu Gln Lys Leu Gln Phe Val Arg Val

145 150 155 160

Phe Glu Leu Glu Asn Ser Met Lys Ser Ile Arg Glu Ser Trp Lys Glu

165 170 175

Lys Lys Asp Glu Glu Gly Lys Val Leu Val Glu Met Lys Lys Trp Phe

180 185 190

Trp Glu Leu Asn Met Asn Ile Val Leu Arg Thr Val Ala Gly Lys Gln

195 200 205

Tyr Thr Gly Thr Val Asp Asp Ala Asp Ala Lys Arg Ile Ser Glu Leu

210 215 220

Phe Arg Glu Trp Phe His Tyr Thr Gly Arg Phe Val Val Gly Asp Ala

225 230 235 240

Phe Pro Phe Leu Gly Trp Leu Asp Leu Gly Gly Tyr Lys Lys Thr Met

245 250 255

Glu Leu Val Ala Ser Arg Leu Asp Ser Met Val Ser Lys Trp Leu Asp

260 265 270

Glu His Arg Lys Lys Gln Ala Asn Asp Asp Lys Lys Glu Asp Met Asp

275 280 285

Phe Met Asp Ile Met Ile Ser Met Thr Glu Ala Asn Ser Pro Leu Glu

290 295 300

Gly Tyr Gly Thr Asp Thr Ile Ile Lys Thr Thr Cys Met Thr Leu Ile

305 310 315 320

Val Ser Gly Val Asp Thr Thr Ser Ile Val Leu Thr Trp Ala Leu Ser

325 330 335

Leu Leu Leu Asn Asn Arg Asp Thr Leu Lys Lys Ala Gln Glu Glu Leu

340 345 350

Asp Met Cys Val Gly Lys Gly Arg Gln Val Asn Glu Ser Asp Leu Val

355 360 365

Asn Leu Ile Tyr Leu Glu Ala Val Leu Lys Glu Ala Leu Arg Leu Tyr

370 375 380

Pro Ala Ala Phe Leu Gly Gly Pro Arg Ala Phe Leu Glu Asp Cys Thr

385 390 395 400

Val Ala Gly Tyr Arg Ile Pro Lys Gly Thr Cys Leu Leu Ile Asn Met

405 410 415

Trp Lys Leu His Arg Asp Pro Asn Ile Trp Ser Asp Pro Cys Glu Phe

420 425 430

Lys Pro Glu Arg Phe Leu Thr Pro Asn Gln Lys Asp Val Asp Val Ile

435 440 445

Gly Met Asp Phe Glu Leu Ile Pro Phe Gly Ala Gly Arg Arg Tyr Cys

450 455 460

Pro Gly Thr Arg Leu Ala Leu Gln Met Leu His Ile Val Leu Ala Thr

465 470 475 480

Leu Leu Gln Asn Phe Glu Met Ser Thr Pro Asn Asp Ala Pro Val Asp

485 490 495

Met Thr Ala Ser Val Gly Met Thr Asn Ala Lys Ala Ser Pro Leu Glu

500 505 510

Val Leu Leu Ser Pro Arg Val Lys Trp Ser

515 520

<210> 71

<211> 1443

<212> DNA

<213> Stevia rebaudiana

<220>

<221> CDS

<222> (1)..(1443)

<400> 71

atg gac gct atg gcc acc act gaa aag aag cct cac gtt atc ttt att 48

Met Asp Ala Met Ala Thr Thr Glu Lys Lys Pro His Val Ile Phe Ile

1 5 10 15

cca ttc cca gct caa tct cat atc aag gct atg ttg aaa ttg gct caa 96

Pro Phe Pro Ala Gln Ser His Ile Lys Ala Met Leu Lys Leu Ala Gln

20 25 30

tta ttg cac cac aag ggt ttg caa atc act ttt gtc aac acc gac ttc 144

Leu Leu His His Lys Gly Leu Gln Ile Thr Phe Val Asn Thr Asp Phe

35 40 45

att cac aac caa ttc ttg gaa tct tct ggt cct cac tgt ttg gac ggt 192

Ile His Asn Gln Phe Leu Glu Ser Ser Gly Pro His Cys Leu Asp Gly

50 55 60

gct cca ggt ttc aga ttc gaa acc att cca gat ggt gtt tcc cac tct 240

Ala Pro Gly Phe Arg Phe Glu Thr Ile Pro Asp Gly Val Ser His Ser

65 70 75 80

cca gaa gcc tcc atc cca atc aga gaa tcc ttg ttg aga tct att gaa 288

Pro Glu Ala Ser Ile Pro Ile Arg Glu Ser Leu Leu Arg Ser Ile Glu

85 90 95

acc aac ttc ttg gac cgt ttc atc gat ttg gtt acc aaa ttg cca gac 336

Thr Asn Phe Leu Asp Arg Phe Ile Asp Leu Val Thr Lys Leu Pro Asp

100 105 110

cca cca acc tgt atc att tct gac ggt ttc ttg tcc gtt ttc acc atc 384

Pro Pro Thr Cys Ile Ile Ser Asp Gly Phe Leu Ser Val Phe Thr Ile

115 120 125

gat gct gcc aag aaa ttg ggt att cca gtc atg atg tac tgg act ttg 432

Asp Ala Ala Lys Lys Leu Gly Ile Pro Val Met Met Tyr Trp Thr Leu

130 135 140

gct gct tgt ggt ttc atg ggt ttc tac cat att cac tct ttg att gaa 480

Ala Ala Cys Gly Phe Met Gly Phe Tyr His Ile His Ser Leu Ile Glu

145 150 155 160

aag ggt ttc gct cca tta aag gat gct tct tac ttg acc aac ggt tac 528

Lys Gly Phe Ala Pro Leu Lys Asp Ala Ser Tyr Leu Thr Asn Gly Tyr

165 170 175

ttg gac acc gtc att gac tgg gtt cca ggt atg gaa ggt atc aga ttg 576

Leu Asp Thr Val Ile Asp Trp Val Pro Gly Met Glu Gly Ile Arg Leu

180 185 190

aaa gat ttc cca ttg gac tgg tct act gac ttg aat gac aag gtc ttg 624

Lys Asp Phe Pro Leu Asp Trp Ser Thr Asp Leu Asn Asp Lys Val Leu

195 200 205

atg ttc act act gaa gct cca caa aga tct cat aag gtt tct cac cac 672

Met Phe Thr Thr Glu Ala Pro Gln Arg Ser His Lys Val Ser His His

210 215 220

atc ttc cac act ttc gat gaa tta gaa cca tct atc atc aag act cta 720

Ile Phe His Thr Phe Asp Glu Leu Glu Pro Ser Ile Ile Lys Thr Leu

225 230 235 240

tcc ttg aga tac aac cat atc tac acc att ggt cca tta caa ttg ttg 768

Ser Leu Arg Tyr Asn His Ile Tyr Thr Ile Gly Pro Leu Gln Leu Leu

245 250 255

ttg gac caa atc cca gaa gaa aag aag caa acc ggt atc act tct ttg 816

Leu Asp Gln Ile Pro Glu Glu Lys Lys Gln Thr Gly Ile Thr Ser Leu

260 265 270

cac ggt tac tct tta gtc aag gaa gaa cca gaa tgt ttc caa tgg tta 864

His Gly Tyr Ser Leu Val Lys Glu Glu Pro Glu Cys Phe Gln Trp Leu

275 280 285

caa tcc aag gaa cca aac tct gtt gtc tac gtt aac ttt ggt tcc acc 912

Gln Ser Lys Glu Pro Asn Ser Val Val Tyr Val Asn Phe Gly Ser Thr

290 295 300

act gtt atg tcc ttg gaa gat atg act gaa ttt ggt tgg ggt ttg gct 960

Thr Val Met Ser Leu Glu Asp Met Thr Glu Phe Gly Trp Gly Leu Ala

305 310 315 320

aac tct aac cac tac ttc tta tgg atc atc aga tct aac ttg gtc att 1008

Asn Ser Asn His Tyr Phe Leu Trp Ile Ile Arg Ser Asn Leu Val Ile

325 330 335

ggt gaa aac gcc gtt ttg cct cca gaa ttg gaa gaa cac atc aag aag 1056

Gly Glu Asn Ala Val Leu Pro Pro Glu Leu Glu Glu His Ile Lys Lys

340 345 350

aga ggt ttc att gct tcc tgg tgt tct caa gaa aag gtc ttg aag cac 1104

Arg Gly Phe Ile Ala Ser Trp Cys Ser Gln Glu Lys Val Leu Lys His

355 360 365

cca tct gtt ggt ggt ttc ttg acc cac tgt ggt tgg ggt tcc acc att 1152

Pro Ser Val Gly Gly Phe Leu Thr His Cys Gly Trp Gly Ser Thr Ile

370 375 380

gaa tcc cta tct gct ggt gtt cca atg atc tgt tgg cca tac tcc tgg 1200

Glu Ser Leu Ser Ala Gly Val Pro Met Ile Cys Trp Pro Tyr Ser Trp

385 390 395 400

gac caa ttg act aac tgt cgt tac atc tgt aag gaa tgg gaa gtt ggt 1248

Asp Gln Leu Thr Asn Cys Arg Tyr Ile Cys Lys Glu Trp Glu Val Gly

405 410 415

ttg gaa atg ggt act aag gtc aag aga gat gaa gtc aag aga tta gtc 1296

Leu Glu Met Gly Thr Lys Val Lys Arg Asp Glu Val Lys Arg Leu Val

420 425 430

caa gaa ttg atg ggt gaa ggt ggt cac aag atg aga aac aaa gcc aag 1344

Gln Glu Leu Met Gly Glu Gly Gly His Lys Met Arg Asn Lys Ala Lys

435 440 445

gac tgg aag gaa aag gcc aga att gct att gct cca aac ggt tct tcc 1392

Asp Trp Lys Glu Lys Ala Arg Ile Ala Ile Ala Pro Asn Gly Ser Ser

450 455 460

tcc ttg aac atc gat aaa atg gtt aag gaa atc act gtc ttg gct cga 1440

Ser Leu Asn Ile Asp Lys Met Val Lys Glu Ile Thr Val Leu Ala Arg

465 470 475 480

aac 1443

Asn

<210> 72

<211> 481

<212> PRT

<213> Stevia rebaudiana

<400> 72

Met Asp Ala Met Ala Thr Thr Glu Lys Lys Pro His Val Ile Phe Ile

1 5 10 15

Pro Phe Pro Ala Gln Ser His Ile Lys Ala Met Leu Lys Leu Ala Gln

20 25 30

Leu Leu His His Lys Gly Leu Gln Ile Thr Phe Val Asn Thr Asp Phe

35 40 45

Ile His Asn Gln Phe Leu Glu Ser Ser Gly Pro His Cys Leu Asp Gly

50 55 60

Ala Pro Gly Phe Arg Phe Glu Thr Ile Pro Asp Gly Val Ser His Ser

65 70 75 80

Pro Glu Ala Ser Ile Pro Ile Arg Glu Ser Leu Leu Arg Ser Ile Glu

85 90 95

Thr Asn Phe Leu Asp Arg Phe Ile Asp Leu Val Thr Lys Leu Pro Asp

100 105 110

Pro Pro Thr Cys Ile Ile Ser Asp Gly Phe Leu Ser Val Phe Thr Ile

115 120 125

Asp Ala Ala Lys Lys Leu Gly Ile Pro Val Met Met Tyr Trp Thr Leu

130 135 140

Ala Ala Cys Gly Phe Met Gly Phe Tyr His Ile His Ser Leu Ile Glu

145 150 155 160

Lys Gly Phe Ala Pro Leu Lys Asp Ala Ser Tyr Leu Thr Asn Gly Tyr

165 170 175

Leu Asp Thr Val Ile Asp Trp Val Pro Gly Met Glu Gly Ile Arg Leu

180 185 190

Lys Asp Phe Pro Leu Asp Trp Ser Thr Asp Leu Asn Asp Lys Val Leu

195 200 205

Met Phe Thr Thr Glu Ala Pro Gln Arg Ser His Lys Val Ser His His

210 215 220

Ile Phe His Thr Phe Asp Glu Leu Glu Pro Ser Ile Ile Lys Thr Leu

225 230 235 240

Ser Leu Arg Tyr Asn His Ile Tyr Thr Ile Gly Pro Leu Gln Leu Leu

245 250 255

Leu Asp Gln Ile Pro Glu Glu Lys Lys Gln Thr Gly Ile Thr Ser Leu

260 265 270

His Gly Tyr Ser Leu Val Lys Glu Glu Pro Glu Cys Phe Gln Trp Leu

275 280 285

Gln Ser Lys Glu Pro Asn Ser Val Val Tyr Val Asn Phe Gly Ser Thr

290 295 300

Thr Val Met Ser Leu Glu Asp Met Thr Glu Phe Gly Trp Gly Leu Ala

305 310 315 320

Asn Ser Asn His Tyr Phe Leu Trp Ile Ile Arg Ser Asn Leu Val Ile

325 330 335

Gly Glu Asn Ala Val Leu Pro Pro Glu Leu Glu Glu His Ile Lys Lys

340 345 350

Arg Gly Phe Ile Ala Ser Trp Cys Ser Gln Glu Lys Val Leu Lys His

355 360 365

Pro Ser Val Gly Gly Phe Leu Thr His Cys Gly Trp Gly Ser Thr Ile

370 375 380

Glu Ser Leu Ser Ala Gly Val Pro Met Ile Cys Trp Pro Tyr Ser Trp

385 390 395 400

Asp Gln Leu Thr Asn Cys Arg Tyr Ile Cys Lys Glu Trp Glu Val Gly

405 410 415

Leu Glu Met Gly Thr Lys Val Lys Arg Asp Glu Val Lys Arg Leu Val

420 425 430

Gln Glu Leu Met Gly Glu Gly Gly His Lys Met Arg Asn Lys Ala Lys

435 440 445

Asp Trp Lys Glu Lys Ala Arg Ile Ala Ile Ala Pro Asn Gly Ser Ser

450 455 460

Ser Leu Asn Ile Asp Lys Met Val Lys Glu Ile Thr Val Leu Ala Arg

465 470 475 480

Asn

<210> 73

<211> 1380

<212> DNA

<213> Stevia rebaudiana

<220>

<221> CDS

<222> (1)..(1380)

<400> 73

atg gct gaa caa caa aag atc aag aaa tct cca cac gtc ttg ttg att 48

Met Ala Glu Gln Gln Lys Ile Lys Lys Ser Pro His Val Leu Leu Ile

1 5 10 15

cca ttc cca ttg caa ggt cac atc aac cca ttc atc caa ttc ggt aag 96

Pro Phe Pro Leu Gln Gly His Ile Asn Pro Phe Ile Gln Phe Gly Lys

20 25 30

aga ttg att tcc aag ggt gtc aag acc act tta gtc acc act att cac 144

Arg Leu Ile Ser Lys Gly Val Lys Thr Thr Leu Val Thr Thr Ile His

35 40 45

act tta aac tcc act tta aac cac tct aac act act acc acc tct att 192

Thr Leu Asn Ser Thr Leu Asn His Ser Asn Thr Thr Thr Thr Ser Ile

50 55 60

gaa atc caa gcc att tct gac ggt tgt gac gaa ggt ggt ttc atg tct 240

Glu Ile Gln Ala Ile Ser Asp Gly Cys Asp Glu Gly Gly Phe Met Ser

65 70 75 80

gct ggt gaa tct tac ttg gaa act ttc aag caa gtc ggt tcc aag tct 288

Ala Gly Glu Ser Tyr Leu Glu Thr Phe Lys Gln Val Gly Ser Lys Ser

85 90 95

ttg gct gat ttg atc aag aaa ttg caa tcc gaa ggt act acc atc gat 336

Leu Ala Asp Leu Ile Lys Lys Leu Gln Ser Glu Gly Thr Thr Ile Asp

100 105 110

gct atc atc tac gac tcc atg act gaa tgg gtt ttg gat gtt gcc att 384

Ala Ile Ile Tyr Asp Ser Met Thr Glu Trp Val Leu Asp Val Ala Ile

115 120 125

gaa ttt ggt att gac ggt ggt tct ttc ttc acc caa gcc tgt gtt gtt 432

Glu Phe Gly Ile Asp Gly Gly Ser Phe Phe Thr Gln Ala Cys Val Val

130 135 140

aac tct ttg tac tac cac gtc cac aag ggt ttg atc tct cta cca tta 480

Asn Ser Leu Tyr Tyr His Val His Lys Gly Leu Ile Ser Leu Pro Leu

145 150 155 160

ggt gaa acc gtt tcc gtc cca ggt ttc cca gtc ttg caa aga tgg gaa 528

Gly Glu Thr Val Ser Val Pro Gly Phe Pro Val Leu Gln Arg Trp Glu

165 170 175

act cca ttg atc tta caa aac cat gaa caa atc caa tct cca tgg tcc 576

Thr Pro Leu Ile Leu Gln Asn His Glu Gln Ile Gln Ser Pro Trp Ser

180 185 190

caa atg ttg ttt ggt caa ttc gct aac att gac caa gct aga tgg gtt 624

Gln Met Leu Phe Gly Gln Phe Ala Asn Ile Asp Gln Ala Arg Trp Val

195 200 205

ttc acc aac tct ttc tac aag ttg gaa gaa gaa gtc att gaa tgg acc 672

Phe Thr Asn Ser Phe Tyr Lys Leu Glu Glu Glu Val Ile Glu Trp Thr

210 215 220

aga aag atc tgg aac ttg aag gtt atc ggt cca act cta cca tcc atg 720

Arg Lys Ile Trp Asn Leu Lys Val Ile Gly Pro Thr Leu Pro Ser Met

225 230 235 240

tac ttg gac aag aga ttg gat gac gac aag gac aac ggt ttc aac ttg 768

Tyr Leu Asp Lys Arg Leu Asp Asp Asp Lys Asp Asn Gly Phe Asn Leu

245 250 255

tac aag gct aac cat cac gaa tgt atg aac tgg ttg gat gac aag cca 816

Tyr Lys Ala Asn His His Glu Cys Met Asn Trp Leu Asp Asp Lys Pro

260 265 270

aag gaa tct gtt gtt tac gtt gct ttc ggt tct ttg gtc aag cat ggt 864

Lys Glu Ser Val Val Tyr Val Ala Phe Gly Ser Leu Val Lys His Gly

275 280 285

cca gaa caa gtt gaa gaa atc acc aga gct ttg att gac tcc gat gtt 912

Pro Glu Gln Val Glu Glu Ile Thr Arg Ala Leu Ile Asp Ser Asp Val

290 295 300

aac ttc tta tgg gtt atc aag cac aag gaa gaa ggt aaa ttg cca gaa 960

Asn Phe Leu Trp Val Ile Lys His Lys Glu Glu Gly Lys Leu Pro Glu

305 310 315 320

aac ttg tct gaa gtt atc aag acc ggt aag ggt ttg att gtt gct tgg 1008

Asn Leu Ser Glu Val Ile Lys Thr Gly Lys Gly Leu Ile Val Ala Trp

325 330 335

tgt aag caa ttg gat gtt ttg gct cac gaa tcc gtc ggt tgt ttc gtc 1056

Cys Lys Gln Leu Asp Val Leu Ala His Glu Ser Val Gly Cys Phe Val

340 345 350

act cac tgt ggt ttc aac tct act ttg gaa gct atc tcc ttg ggt gtt 1104

Thr His Cys Gly Phe Asn Ser Thr Leu Glu Ala Ile Ser Leu Gly Val

355 360 365

cca gtt gtt gcc atg cct caa ttc tct gac caa acc acc aac gcc aaa 1152

Pro Val Val Ala Met Pro Gln Phe Ser Asp Gln Thr Thr Asn Ala Lys

370 375 380

ttg ttg gat gaa atc ttg ggt gtc ggt gtc cgt gtc aag gct gat gaa 1200

Leu Leu Asp Glu Ile Leu Gly Val Gly Val Arg Val Lys Ala Asp Glu

385 390 395 400

aac ggt att gtt aga aga ggt aac tta gct tcc tgt atc aag atg atc 1248

Asn Gly Ile Val Arg Arg Gly Asn Leu Ala Ser Cys Ile Lys Met Ile

405 410 415

atg gaa gaa gaa cgt ggt gtc att atc aga aag aat gct gtc aaa tgg 1296

Met Glu Glu Glu Arg Gly Val Ile Ile Arg Lys Asn Ala Val Lys Trp

420 425 430

aag gac ttg gct aag gtt gct gtc cac gaa ggt ggt tcc tct gac aat 1344

Lys Asp Leu Ala Lys Val Ala Val His Glu Gly Gly Ser Ser Asp Asn

435 440 445

gac att gtt gaa ttt gtc tct gaa ttg atc aaa gcg 1380

Asp Ile Val Glu Phe Val Ser Glu Leu Ile Lys Ala

450 455 460

<210> 74

<211> 460

<212> PRT

<213> Stevia rebaudiana

<400> 74

Met Ala Glu Gln Gln Lys Ile Lys Lys Ser Pro His Val Leu Leu Ile

1 5 10 15

Pro Phe Pro Leu Gln Gly His Ile Asn Pro Phe Ile Gln Phe Gly Lys

20 25 30

Arg Leu Ile Ser Lys Gly Val Lys Thr Thr Leu Val Thr Thr Ile His

35 40 45

Thr Leu Asn Ser Thr Leu Asn His Ser Asn Thr Thr Thr Thr Ser Ile

50 55 60

Glu Ile Gln Ala Ile Ser Asp Gly Cys Asp Glu Gly Gly Phe Met Ser

65 70 75 80

Ala Gly Glu Ser Tyr Leu Glu Thr Phe Lys Gln Val Gly Ser Lys Ser

85 90 95

Leu Ala Asp Leu Ile Lys Lys Leu Gln Ser Glu Gly Thr Thr Ile Asp

100 105 110

Ala Ile Ile Tyr Asp Ser Met Thr Glu Trp Val Leu Asp Val Ala Ile

115 120 125

Glu Phe Gly Ile Asp Gly Gly Ser Phe Phe Thr Gln Ala Cys Val Val

130 135 140

Asn Ser Leu Tyr Tyr His Val His Lys Gly Leu Ile Ser Leu Pro Leu

145 150 155 160

Gly Glu Thr Val Ser Val Pro Gly Phe Pro Val Leu Gln Arg Trp Glu

165 170 175

Thr Pro Leu Ile Leu Gln Asn His Glu Gln Ile Gln Ser Pro Trp Ser

180 185 190

Gln Met Leu Phe Gly Gln Phe Ala Asn Ile Asp Gln Ala Arg Trp Val

195 200 205

Phe Thr Asn Ser Phe Tyr Lys Leu Glu Glu Glu Val Ile Glu Trp Thr

210 215 220

Arg Lys Ile Trp Asn Leu Lys Val Ile Gly Pro Thr Leu Pro Ser Met

225 230 235 240

Tyr Leu Asp Lys Arg Leu Asp Asp Asp Lys Asp Asn Gly Phe Asn Leu

245 250 255

Tyr Lys Ala Asn His His Glu Cys Met Asn Trp Leu Asp Asp Lys Pro

260 265 270

Lys Glu Ser Val Val Tyr Val Ala Phe Gly Ser Leu Val Lys His Gly

275 280 285

Pro Glu Gln Val Glu Glu Ile Thr Arg Ala Leu Ile Asp Ser Asp Val

290 295 300

Asn Phe Leu Trp Val Ile Lys His Lys Glu Glu Gly Lys Leu Pro Glu

305 310 315 320

Asn Leu Ser Glu Val Ile Lys Thr Gly Lys Gly Leu Ile Val Ala Trp

325 330 335

Cys Lys Gln Leu Asp Val Leu Ala His Glu Ser Val Gly Cys Phe Val

340 345 350

Thr His Cys Gly Phe Asn Ser Thr Leu Glu Ala Ile Ser Leu Gly Val

355 360 365

Pro Val Val Ala Met Pro Gln Phe Ser Asp Gln Thr Thr Asn Ala Lys

370 375 380

Leu Leu Asp Glu Ile Leu Gly Val Gly Val Arg Val Lys Ala Asp Glu

385 390 395 400

Asn Gly Ile Val Arg Arg Gly Asn Leu Ala Ser Cys Ile Lys Met Ile

405 410 415

Met Glu Glu Glu Arg Gly Val Ile Ile Arg Lys Asn Ala Val Lys Trp

420 425 430

Lys Asp Leu Ala Lys Val Ala Val His Glu Gly Gly Ser Ser Asp Asn

435 440 445

Asp Ile Val Glu Phe Val Ser Glu Leu Ile Lys Ala

450 455 460

<210> 75

<211> 1374

<212> DNA

<213> Stevia rebaudiana

<220>

<221> CDS

<222> (1)..(1374)

<400> 75

atg gaa aac aag act gaa acc act gtt aga aga aga aga aga atc atc 48

Met Glu Asn Lys Thr Glu Thr Thr Val Arg Arg Arg Arg Arg Ile Ile

1 5 10 15

tta ttc cca gtt cca ttc caa ggt cac att aac cca atc ttg caa ttg 96

Leu Phe Pro Val Pro Phe Gln Gly His Ile Asn Pro Ile Leu Gln Leu

20 25 30

gct aac gtc tta tac tcc aag ggt ttc tcc atc acc atc ttc cac acc 144

Ala Asn Val Leu Tyr Ser Lys Gly Phe Ser Ile Thr Ile Phe His Thr

35 40 45

aac ttc aac aaa cct aaa act tcc aac tac cca cac ttc acc ttc aga 192

Asn Phe Asn Lys Pro Lys Thr Ser Asn Tyr Pro His Phe Thr Phe Arg

50 55 60

ttt atc ttg gac aac gac cca caa gat gaa aga att tct aac ttg cca 240

Phe Ile Leu Asp Asn Asp Pro Gln Asp Glu Arg Ile Ser Asn Leu Pro

65 70 75 80

acc cat ggt cca ttg gcc ggt atg aga att cca atc atc aac gaa cac 288

Thr His Gly Pro Leu Ala Gly Met Arg Ile Pro Ile Ile Asn Glu His

85 90 95

ggt gct gac gaa ttg aga aga gaa ttg gaa ttg ttg atg ttg gct tct 336

Gly Ala Asp Glu Leu Arg Arg Glu Leu Glu Leu Leu Met Leu Ala Ser

100 105 110

gaa gaa gat gaa gaa gtc tct tgt ttg atc act gat gct tta tgg tac 384

Glu Glu Asp Glu Glu Val Ser Cys Leu Ile Thr Asp Ala Leu Trp Tyr

115 120 125

ttt gct caa tct gtt gct gac tct ttg aac ttg aga aga tta gtc ttg 432

Phe Ala Gln Ser Val Ala Asp Ser Leu Asn Leu Arg Arg Leu Val Leu

130 135 140

atg acc tct tct ttg ttc aac ttc cac gct cac gtt tct cta cca caa 480

Met Thr Ser Ser Leu Phe Asn Phe His Ala His Val Ser Leu Pro Gln

145 150 155 160

ttt gat gaa ttg ggt tac ttg gac cca gat gac aag acc aga ttg gaa 528

Phe Asp Glu Leu Gly Tyr Leu Asp Pro Asp Asp Lys Thr Arg Leu Glu

165 170 175

gaa caa gcc tcc ggt ttc cca atg ttg aag gtc aag gat atc aag tct 576

Glu Gln Ala Ser Gly Phe Pro Met Leu Lys Val Lys Asp Ile Lys Ser

180 185 190

gcc tac tcc aac tgg caa atc ttg aag gaa att ttg ggt aag atg atc 624

Ala Tyr Ser Asn Trp Gln Ile Leu Lys Glu Ile Leu Gly Lys Met Ile

195 200 205

aag caa acc aag gct tct tct ggt gtc atc tgg aac tcc ttc aag gaa 672

Lys Gln Thr Lys Ala Ser Ser Gly Val Ile Trp Asn Ser Phe Lys Glu

210 215 220

ttg gaa gaa tct gaa ttg gaa acc gtc atc aga gaa att cca gct cca 720

Leu Glu Glu Ser Glu Leu Glu Thr Val Ile Arg Glu Ile Pro Ala Pro

225 230 235 240

tct ttc ttg att cca tta cca aag cat ttg act gct tcc tcc tct tct 768

Ser Phe Leu Ile Pro Leu Pro Lys His Leu Thr Ala Ser Ser Ser Ser

245 250 255

cta ttg gac cac gac aga act gtt ttc caa tgg ttg gac caa caa cca 816

Leu Leu Asp His Asp Arg Thr Val Phe Gln Trp Leu Asp Gln Gln Pro

260 265 270

cca tct tcc gtc tta tac gtt tcc ttt ggt tcc act tct gaa gtt gac 864

Pro Ser Ser Val Leu Tyr Val Ser Phe Gly Ser Thr Ser Glu Val Asp

275 280 285

gaa aag gac ttc ttg gaa att gct cgt ggt ttg gtt gac tcc aag caa 912

Glu Lys Asp Phe Leu Glu Ile Ala Arg Gly Leu Val Asp Ser Lys Gln

290 295 300

tct ttc tta tgg gtt gtc aga cca ggt ttc gtc aag ggt tcc acc tgg 960

Ser Phe Leu Trp Val Val Arg Pro Gly Phe Val Lys Gly Ser Thr Trp

305 310 315 320

gtt gaa cct ttg cca gac ggt ttc ttg ggt gaa aga ggt aga att gtc 1008

Val Glu Pro Leu Pro Asp Gly Phe Leu Gly Glu Arg Gly Arg Ile Val

325 330 335

aaa tgg gtt cca caa caa gaa gtt ttg gct cac ggt gcc att ggt gct 1056

Lys Trp Val Pro Gln Gln Glu Val Leu Ala His Gly Ala Ile Gly Ala

340 345 350

ttc tgg act cac tct ggt tgg aac tct act ttg gaa tcc gtt tgt gaa 1104

Phe Trp Thr His Ser Gly Trp Asn Ser Thr Leu Glu Ser Val Cys Glu

355 360 365

ggt gtt cca atg att ttc tct gac ttc ggt ttg gac caa cca ttg aat 1152

Gly Val Pro Met Ile Phe Ser Asp Phe Gly Leu Asp Gln Pro Leu Asn

370 375 380

gct cgt tac atg tcc gat gtt ttg aag gtt ggt gtc tac ttg gaa aac 1200

Ala Arg Tyr Met Ser Asp Val Leu Lys Val Gly Val Tyr Leu Glu Asn

385 390 395 400

ggt tgg gaa cgt ggt gaa att gct aac gcc atc aga aga gtc atg gtc 1248

Gly Trp Glu Arg Gly Glu Ile Ala Asn Ala Ile Arg Arg Val Met Val

405 410 415

gat gaa gaa ggt gaa tac atc aga caa aat gct cgt gtc ttg aaa caa 1296

Asp Glu Glu Gly Glu Tyr Ile Arg Gln Asn Ala Arg Val Leu Lys Gln

420 425 430

aag gct gat gtt tct ttg atg aag ggt ggt tct tct tac gaa tct ttg 1344

Lys Ala Asp Val Ser Leu Met Lys Gly Gly Ser Ser Tyr Glu Ser Leu

435 440 445

gaa tct ttg gtt tcc tac atc tcc agt ctc 1374

Glu Ser Leu Val Ser Tyr Ile Ser Ser Leu

450 455

<210> 76

<211> 458

<212> PRT

<213> Stevia rebaudiana

<400> 76

Met Glu Asn Lys Thr Glu Thr Thr Val Arg Arg Arg Arg Arg Ile Ile

1 5 10 15

Leu Phe Pro Val Pro Phe Gln Gly His Ile Asn Pro Ile Leu Gln Leu

20 25 30

Ala Asn Val Leu Tyr Ser Lys Gly Phe Ser Ile Thr Ile Phe His Thr

35 40 45

Asn Phe Asn Lys Pro Lys Thr Ser Asn Tyr Pro His Phe Thr Phe Arg

50 55 60

Phe Ile Leu Asp Asn Asp Pro Gln Asp Glu Arg Ile Ser Asn Leu Pro

65 70 75 80

Thr His Gly Pro Leu Ala Gly Met Arg Ile Pro Ile Ile Asn Glu His

85 90 95

Gly Ala Asp Glu Leu Arg Arg Glu Leu Glu Leu Leu Met Leu Ala Ser

100 105 110

Glu Glu Asp Glu Glu Val Ser Cys Leu Ile Thr Asp Ala Leu Trp Tyr

115 120 125

Phe Ala Gln Ser Val Ala Asp Ser Leu Asn Leu Arg Arg Leu Val Leu

130 135 140

Met Thr Ser Ser Leu Phe Asn Phe His Ala His Val Ser Leu Pro Gln

145 150 155 160

Phe Asp Glu Leu Gly Tyr Leu Asp Pro Asp Asp Lys Thr Arg Leu Glu

165 170 175

Glu Gln Ala Ser Gly Phe Pro Met Leu Lys Val Lys Asp Ile Lys Ser

180 185 190

Ala Tyr Ser Asn Trp Gln Ile Leu Lys Glu Ile Leu Gly Lys Met Ile

195 200 205

Lys Gln Thr Lys Ala Ser Ser Gly Val Ile Trp Asn Ser Phe Lys Glu

210 215 220

Leu Glu Glu Ser Glu Leu Glu Thr Val Ile Arg Glu Ile Pro Ala Pro

225 230 235 240

Ser Phe Leu Ile Pro Leu Pro Lys His Leu Thr Ala Ser Ser Ser Ser

245 250 255

Leu Leu Asp His Asp Arg Thr Val Phe Gln Trp Leu Asp Gln Gln Pro

260 265 270

Pro Ser Ser Val Leu Tyr Val Ser Phe Gly Ser Thr Ser Glu Val Asp

275 280 285

Glu Lys Asp Phe Leu Glu Ile Ala Arg Gly Leu Val Asp Ser Lys Gln

290 295 300

Ser Phe Leu Trp Val Val Arg Pro Gly Phe Val Lys Gly Ser Thr Trp

305 310 315 320

Val Glu Pro Leu Pro Asp Gly Phe Leu Gly Glu Arg Gly Arg Ile Val

325 330 335

Lys Trp Val Pro Gln Gln Glu Val Leu Ala His Gly Ala Ile Gly Ala

340 345 350

Phe Trp Thr His Ser Gly Trp Asn Ser Thr Leu Glu Ser Val Cys Glu

355 360 365

Gly Val Pro Met Ile Phe Ser Asp Phe Gly Leu Asp Gln Pro Leu Asn

370 375 380

Ala Arg Tyr Met Ser Asp Val Leu Lys Val Gly Val Tyr Leu Glu Asn

385 390 395 400

Gly Trp Glu Arg Gly Glu Ile Ala Asn Ala Ile Arg Arg Val Met Val

405 410 415

Asp Glu Glu Gly Glu Tyr Ile Arg Gln Asn Ala Arg Val Leu Lys Gln

420 425 430

Lys Ala Asp Val Ser Leu Met Lys Gly Gly Ser Ser Tyr Glu Ser Leu

435 440 445

Glu Ser Leu Val Ser Tyr Ile Ser Ser Leu

450 455

<210> 77

<211> 2130

<212> DNA

<213> Stevia rebaudiana

<220>

<221> CDS

<222> (1)..(2130)

<400> 77

atg caa tct gat tcc gtt aag gtt tct cca ttc gac tta gtc tct gct 48

Met Gln Ser Asp Ser Val Lys Val Ser Pro Phe Asp Leu Val Ser Ala

1 5 10 15

gcc atg aac ggt aag gct atg gaa aag ttg aac gct tct gaa tct gag 96

Ala Met Asn Gly Lys Ala Met Glu Lys Leu Asn Ala Ser Glu Ser Glu

20 25 30

gac cca act act tta cca gct tta aag atg ttg gtt gaa aac aga gaa 144

Asp Pro Thr Thr Leu Pro Ala Leu Lys Met Leu Val Glu Asn Arg Glu

35 40 45

ttg ttg act tta ttc acc acc tcc ttt gct gtc ttg att ggt tgt ttg 192

Leu Leu Thr Leu Phe Thr Thr Ser Phe Ala Val Leu Ile Gly Cys Leu

50 55 60

gtt ttc ttg atg tgg aga aga tct tct tct aag aag ttg gtt caa gac 240

Val Phe Leu Met Trp Arg Arg Ser Ser Ser Lys Lys Leu Val Gln Asp

65 70 75 80

cca gtt cct caa gtc atc gtc gtc aag aag aag gaa aag gaa tct gaa 288

Pro Val Pro Gln Val Ile Val Val Lys Lys Lys Glu Lys Glu Ser Glu

85 90 95

gtc gat gac ggt aag aag aaa gtt tcc atc ttc tac ggt act caa acc 336

Val Asp Asp Gly Lys Lys Lys Val Ser Ile Phe Tyr Gly Thr Gln Thr

100 105 110

ggt act gct gaa ggt ttc gct aaa gct tta gtt gaa gaa gct aag gtc 384

Gly Thr Ala Glu Gly Phe Ala Lys Ala Leu Val Glu Glu Ala Lys Val

115 120 125

aga tac gaa aag acc tct ttc aag gtt atc gat ttg gac gac tac gcc 432

Arg Tyr Glu Lys Thr Ser Phe Lys Val Ile Asp Leu Asp Asp Tyr Ala

130 135 140

gct gac gac gac gaa tac gaa gaa aag ttg aag aag gaa tct cta gct 480

Ala Asp Asp Asp Glu Tyr Glu Glu Lys Leu Lys Lys Glu Ser Leu Ala

145 150 155 160

ttc ttc ttc ttg gct acc tac ggt gac ggt gaa cca acc gac aat gct 528

Phe Phe Phe Leu Ala Thr Tyr Gly Asp Gly Glu Pro Thr Asp Asn Ala

165 170 175

gcc aac ttc tac aaa tgg ttc acc gaa ggt gat gac aag ggt gaa tgg 576

Ala Asn Phe Tyr Lys Trp Phe Thr Glu Gly Asp Asp Lys Gly Glu Trp

180 185 190

ttg aag aaa ttg caa tac ggt gtt ttc ggt tta ggt aac aga caa tac 624

Leu Lys Lys Leu Gln Tyr Gly Val Phe Gly Leu Gly Asn Arg Gln Tyr

195 200 205

gaa cac ttc aac aag att gcc atc gtc gtt gat gac aaa ttg act gaa 672

Glu His Phe Asn Lys Ile Ala Ile Val Val Asp Asp Lys Leu Thr Glu

210 215 220

atg ggt gcc aag aga ttg gtt cca gtc ggt ttg ggt gat gat gac caa 720

Met Gly Ala Lys Arg Leu Val Pro Val Gly Leu Gly Asp Asp Asp Gln

225 230 235 240

tgt att gaa gat gac ttt acc gct tgg aag gaa tta gtt tgg cca gaa 768

Cys Ile Glu Asp Asp Phe Thr Ala Trp Lys Glu Leu Val Trp Pro Glu

245 250 255

tta gac caa ttg ttg aga gat gaa gat gac acc tct gtt acc acc cca 816

Leu Asp Gln Leu Leu Arg Asp Glu Asp Asp Thr Ser Val Thr Thr Pro

260 265 270

tac acc gcc gct gtc ttg gaa tac cgt gtc gtt tac cat gac aaa cca 864

Tyr Thr Ala Ala Val Leu Glu Tyr Arg Val Val Tyr His Asp Lys Pro

275 280 285

gct gac tct tac gct gaa gat caa acc cac acc aac ggt cac gtt gtc 912

Ala Asp Ser Tyr Ala Glu Asp Gln Thr His Thr Asn Gly His Val Val

290 295 300

cac gac gct caa cac cca tcc aga tct aac gtt gct ttc aag aag gaa 960

His Asp Ala Gln His Pro Ser Arg Ser Asn Val Ala Phe Lys Lys Glu

305 310 315 320

ttg cac act tcc caa tct gat cgt tct tgt acc cac ttg gaa ttt gac 1008

Leu His Thr Ser Gln Ser Asp Arg Ser Cys Thr His Leu Glu Phe Asp

325 330 335

atc tcc cac acc ggt ttg tct tac gaa acc ggt gac cat gtt ggt gtc 1056

Ile Ser His Thr Gly Leu Ser Tyr Glu Thr Gly Asp His Val Gly Val

340 345 350

tac tct gaa aac ttg tct gaa gtt gtc gac gaa gct ttg aaa ttg ttg 1104

Tyr Ser Glu Asn Leu Ser Glu Val Val Asp Glu Ala Leu Lys Leu Leu

355 360 365

ggt ttg tct cca gac act tac ttc tcc gtc cac gct gac aaa gaa gat 1152

Gly Leu Ser Pro Asp Thr Tyr Phe Ser Val His Ala Asp Lys Glu Asp

370 375 380

ggt act cca atc ggt ggt gct tct ttg cct cca cca ttc cca cca tgt 1200

Gly Thr Pro Ile Gly Gly Ala Ser Leu Pro Pro Pro Phe Pro Pro Cys

385 390 395 400

act cta aga gat gct ttg acc cgt tat gct gac gtt cta tcc tct cca 1248

Thr Leu Arg Asp Ala Leu Thr Arg Tyr Ala Asp Val Leu Ser Ser Pro

405 410 415

aag aag gtt gct ttg ttg gct ttg gct gct cat gct tct gat cca tct 1296

Lys Lys Val Ala Leu Leu Ala Leu Ala Ala His Ala Ser Asp Pro Ser

420 425 430

gaa gct gac aga ttg aag ttc ttg gct tcc cca gcc ggt aag gac gaa 1344

Glu Ala Asp Arg Leu Lys Phe Leu Ala Ser Pro Ala Gly Lys Asp Glu

435 440 445

tac gct caa tgg att gtt gcc aac caa aga tcc ttg ttg gaa gtc atg 1392

Tyr Ala Gln Trp Ile Val Ala Asn Gln Arg Ser Leu Leu Glu Val Met

450 455 460

caa tct ttc cca tct gcc aag cct cca ttg ggt gtt ttc ttt gcc gct 1440

Gln Ser Phe Pro Ser Ala Lys Pro Pro Leu Gly Val Phe Phe Ala Ala

465 470 475 480

gtt gcc cca aga ttg caa cca aga tac tac tcc atc tcc tct tct cca 1488

Val Ala Pro Arg Leu Gln Pro Arg Tyr Tyr Ser Ile Ser Ser Ser Pro

485 490 495

aag atg tcc cca aac aga att cac gtc act tgt gct ttg gtt tac gaa 1536

Lys Met Ser Pro Asn Arg Ile His Val Thr Cys Ala Leu Val Tyr Glu

500 505 510

acc act cca gct ggt aga att cac aga ggt ttg tgt tcc acc tgg atg 1584

Thr Thr Pro Ala Gly Arg Ile His Arg Gly Leu Cys Ser Thr Trp Met

515 520 525

aag aat gcc gtc cca tta act gaa tct cca gac tgt tcc caa gct tct 1632

Lys Asn Ala Val Pro Leu Thr Glu Ser Pro Asp Cys Ser Gln Ala Ser

530 535 540

atc ttc gtc aga act tcc aac ttc aga ttg cca gtc gat cca aag gtc 1680

Ile Phe Val Arg Thr Ser Asn Phe Arg Leu Pro Val Asp Pro Lys Val

545 550 555 560

cca gtt atc atg atc ggt cca ggt act ggt ttg gct cca ttc aga ggt 1728

Pro Val Ile Met Ile Gly Pro Gly Thr Gly Leu Ala Pro Phe Arg Gly

565 570 575

ttc ttg caa gaa aga tta gct ttg aaa gaa tct ggt act gaa ttg ggt 1776

Phe Leu Gln Glu Arg Leu Ala Leu Lys Glu Ser Gly Thr Glu Leu Gly

580 585 590

tcc tcc atc ttc ttc ttc ggt tgt aga aac aga aag gtc gat ttt atc 1824

Ser Ser Ile Phe Phe Phe Gly Cys Arg Asn Arg Lys Val Asp Phe Ile

595 600 605

tac gaa gat gaa ttg aac aac ttc gtt gaa act ggt gct ttg tct gaa 1872

Tyr Glu Asp Glu Leu Asn Asn Phe Val Glu Thr Gly Ala Leu Ser Glu

610 615 620

ttg att gtc gct ttc tcc aga gaa ggt act gcc aag gaa tac gtc caa 1920

Leu Ile Val Ala Phe Ser Arg Glu Gly Thr Ala Lys Glu Tyr Val Gln

625 630 635 640

cac aag atg tcc caa aag gct tct gat atc tgg aag ttg ttg tct gaa 1968

His Lys Met Ser Gln Lys Ala Ser Asp Ile Trp Lys Leu Leu Ser Glu

645 650 655

ggt gct tac ttg tac gtt tgt ggt gat gct aaa ggt atg gcc aag gac 2016

Gly Ala Tyr Leu Tyr Val Cys Gly Asp Ala Lys Gly Met Ala Lys Asp

660 665 670

gtt cac aga act ttg cac act att gtt caa gaa caa ggt tct ttg gac 2064

Val His Arg Thr Leu His Thr Ile Val Gln Glu Gln Gly Ser Leu Asp

675 680 685

tcc tcc aag gct gaa tta tac gtc aag aac ttg caa atg tcc ggt cgt 2112

Ser Ser Lys Ala Glu Leu Tyr Val Lys Asn Leu Gln Met Ser Gly Arg

690 695 700

tac ttg aga gat gta tgg 2130

Tyr Leu Arg Asp Val Trp

705 710

<210> 78

<211> 710

<212> PRT

<213> Stevia rebaudiana

<400> 78

Met Gln Ser Asp Ser Val Lys Val Ser Pro Phe Asp Leu Val Ser Ala

1 5 10 15

Ala Met Asn Gly Lys Ala Met Glu Lys Leu Asn Ala Ser Glu Ser Glu

20 25 30

Asp Pro Thr Thr Leu Pro Ala Leu Lys Met Leu Val Glu Asn Arg Glu

35 40 45

Leu Leu Thr Leu Phe Thr Thr Ser Phe Ala Val Leu Ile Gly Cys Leu

50 55 60

Val Phe Leu Met Trp Arg Arg Ser Ser Ser Lys Lys Leu Val Gln Asp

65 70 75 80

Pro Val Pro Gln Val Ile Val Val Lys Lys Lys Glu Lys Glu Ser Glu

85 90 95

Val Asp Asp Gly Lys Lys Lys Val Ser Ile Phe Tyr Gly Thr Gln Thr

100 105 110

Gly Thr Ala Glu Gly Phe Ala Lys Ala Leu Val Glu Glu Ala Lys Val

115 120 125

Arg Tyr Glu Lys Thr Ser Phe Lys Val Ile Asp Leu Asp Asp Tyr Ala

130 135 140

Ala Asp Asp Asp Glu Tyr Glu Glu Lys Leu Lys Lys Glu Ser Leu Ala

145 150 155 160

Phe Phe Phe Leu Ala Thr Tyr Gly Asp Gly Glu Pro Thr Asp Asn Ala

165 170 175

Ala Asn Phe Tyr Lys Trp Phe Thr Glu Gly Asp Asp Lys Gly Glu Trp

180 185 190

Leu Lys Lys Leu Gln Tyr Gly Val Phe Gly Leu Gly Asn Arg Gln Tyr

195 200 205

Glu His Phe Asn Lys Ile Ala Ile Val Val Asp Asp Lys Leu Thr Glu

210 215 220

Met Gly Ala Lys Arg Leu Val Pro Val Gly Leu Gly Asp Asp Asp Gln

225 230 235 240

Cys Ile Glu Asp Asp Phe Thr Ala Trp Lys Glu Leu Val Trp Pro Glu

245 250 255

Leu Asp Gln Leu Leu Arg Asp Glu Asp Asp Thr Ser Val Thr Thr Pro

260 265 270

Tyr Thr Ala Ala Val Leu Glu Tyr Arg Val Val Tyr His Asp Lys Pro

275 280 285

Ala Asp Ser Tyr Ala Glu Asp Gln Thr His Thr Asn Gly His Val Val

290 295 300

His Asp Ala Gln His Pro Ser Arg Ser Asn Val Ala Phe Lys Lys Glu

305 310 315 320

Leu His Thr Ser Gln Ser Asp Arg Ser Cys Thr His Leu Glu Phe Asp

325 330 335

Ile Ser His Thr Gly Leu Ser Tyr Glu Thr Gly Asp His Val Gly Val

340 345 350

Tyr Ser Glu Asn Leu Ser Glu Val Val Asp Glu Ala Leu Lys Leu Leu

355 360 365

Gly Leu Ser Pro Asp Thr Tyr Phe Ser Val His Ala Asp Lys Glu Asp

370 375 380

Gly Thr Pro Ile Gly Gly Ala Ser Leu Pro Pro Pro Phe Pro Pro Cys

385 390 395 400

Thr Leu Arg Asp Ala Leu Thr Arg Tyr Ala Asp Val Leu Ser Ser Pro

405 410 415

Lys Lys Val Ala Leu Leu Ala Leu Ala Ala His Ala Ser Asp Pro Ser

420 425 430

Glu Ala Asp Arg Leu Lys Phe Leu Ala Ser Pro Ala Gly Lys Asp Glu

435 440 445

Tyr Ala Gln Trp Ile Val Ala Asn Gln Arg Ser Leu Leu Glu Val Met

450 455 460

Gln Ser Phe Pro Ser Ala Lys Pro Pro Leu Gly Val Phe Phe Ala Ala

465 470 475 480

Val Ala Pro Arg Leu Gln Pro Arg Tyr Tyr Ser Ile Ser Ser Ser Pro

485 490 495

Lys Met Ser Pro Asn Arg Ile His Val Thr Cys Ala Leu Val Tyr Glu

500 505 510

Thr Thr Pro Ala Gly Arg Ile His Arg Gly Leu Cys Ser Thr Trp Met

515 520 525

Lys Asn Ala Val Pro Leu Thr Glu Ser Pro Asp Cys Ser Gln Ala Ser

530 535 540

Ile Phe Val Arg Thr Ser Asn Phe Arg Leu Pro Val Asp Pro Lys Val

545 550 555 560

Pro Val Ile Met Ile Gly Pro Gly Thr Gly Leu Ala Pro Phe Arg Gly

565 570 575

Phe Leu Gln Glu Arg Leu Ala Leu Lys Glu Ser Gly Thr Glu Leu Gly

580 585 590

Ser Ser Ile Phe Phe Phe Gly Cys Arg Asn Arg Lys Val Asp Phe Ile

595 600 605

Tyr Glu Asp Glu Leu Asn Asn Phe Val Glu Thr Gly Ala Leu Ser Glu

610 615 620

Leu Ile Val Ala Phe Ser Arg Glu Gly Thr Ala Lys Glu Tyr Val Gln

625 630 635 640

His Lys Met Ser Gln Lys Ala Ser Asp Ile Trp Lys Leu Leu Ser Glu

645 650 655

Gly Ala Tyr Leu Tyr Val Cys Gly Asp Ala Lys Gly Met Ala Lys Asp

660 665 670

Val His Arg Thr Leu His Thr Ile Val Gln Glu Gln Gly Ser Leu Asp

675 680 685

Ser Ser Lys Ala Glu Leu Tyr Val Lys Asn Leu Gln Met Ser Gly Arg

690 695 700

Tyr Leu Arg Asp Val Trp

705 710

<210> 79

<211> 1575

<212> DNA

<213> Saccharomyces cerevisiae

<220>

<221> CDS

<222> (1)..(1575)

<400> 79

atg gac caa ttg gtc aag act gaa gtc acc aag aaa tct ttc act gct 48

Met Asp Gln Leu Val Lys Thr Glu Val Thr Lys Lys Ser Phe Thr Ala

1 5 10 15

cca gtc caa aag gct tcc act cca gtt ttg acc aac aag acc gtc atc 96

Pro Val Gln Lys Ala Ser Thr Pro Val Leu Thr Asn Lys Thr Val Ile

20 25 30

tcc ggt tcc aag gtt aaa tct ttg tcc tct gct caa tct tcc tcc tct 144

Ser Gly Ser Lys Val Lys Ser Leu Ser Ser Ala Gln Ser Ser Ser Ser

35 40 45

ggt cca tct tct tct tct gaa gaa gat gat tcc aga gat atc gaa tct 192

Gly Pro Ser Ser Ser Ser Glu Glu Asp Asp Ser Arg Asp Ile Glu Ser

50 55 60

ttg gac aag aaa atc aga cca ttg gaa gaa ttg gaa gct cta ttg tcc 240

Leu Asp Lys Lys Ile Arg Pro Leu Glu Glu Leu Glu Ala Leu Leu Ser

65 70 75 80

tct ggt aac act aag caa tta aag aac aag gaa gtt gct gct ttg gtt 288

Ser Gly Asn Thr Lys Gln Leu Lys Asn Lys Glu Val Ala Ala Leu Val

85 90 95

atc cac ggt aaa ttg cca ttg tac gct ttg gaa aag aaa tta ggt gac 336

Ile His Gly Lys Leu Pro Leu Tyr Ala Leu Glu Lys Lys Leu Gly Asp

100 105 110

acc acc aga gct gtt gct gtc aga aga aag gct ttg tcc att ttg gct 384

Thr Thr Arg Ala Val Ala Val Arg Arg Lys Ala Leu Ser Ile Leu Ala

115 120 125

gaa gct cca gtc ttg gct tcc gac aga tta cca tac aag aac tac gac 432

Glu Ala Pro Val Leu Ala Ser Asp Arg Leu Pro Tyr Lys Asn Tyr Asp

130 135 140

tac gac cgt gtc ttt ggt gct tgt tgt gaa aat gtc att ggt tac atg 480

Tyr Asp Arg Val Phe Gly Ala Cys Cys Glu Asn Val Ile Gly Tyr Met

145 150 155 160

cca tta cca gtt ggt gtc att ggt cca ttg gtt atc gac ggt act tct 528

Pro Leu Pro Val Gly Val Ile Gly Pro Leu Val Ile Asp Gly Thr Ser

165 170 175

tac cac atc cca atg gct acc act gaa ggt tgt ttg gtt gct tct gcc 576

Tyr His Ile Pro Met Ala Thr Thr Glu Gly Cys Leu Val Ala Ser Ala

180 185 190

atg aga ggt tgt aag gcc atc aac gct ggt ggt ggt gct acc acc gtt 624

Met Arg Gly Cys Lys Ala Ile Asn Ala Gly Gly Gly Ala Thr Thr Val

195 200 205

ttg act aag gat ggt atg acc aga ggt cct gtt gtc aga ttc cca act 672

Leu Thr Lys Asp Gly Met Thr Arg Gly Pro Val Val Arg Phe Pro Thr

210 215 220

ttg aag aga tct ggt gct tgt aag atc tgg ttg gat tct gaa gaa ggt 720

Leu Lys Arg Ser Gly Ala Cys Lys Ile Trp Leu Asp Ser Glu Glu Gly

225 230 235 240

caa aac gcc atc aag aag gct ttc aac tcc act tcc aga ttc gct aga 768

Gln Asn Ala Ile Lys Lys Ala Phe Asn Ser Thr Ser Arg Phe Ala Arg

245 250 255

ttg caa cac att caa act tgt tta gct ggt gac ttg ttg ttc atg aga 816

Leu Gln His Ile Gln Thr Cys Leu Ala Gly Asp Leu Leu Phe Met Arg

260 265 270

ttc aga acc acc act ggt gac gct atg ggt atg aac atg atc tcc aag 864

Phe Arg Thr Thr Thr Gly Asp Ala Met Gly Met Asn Met Ile Ser Lys

275 280 285

ggt gtt gaa tac tct ttg aag caa atg gtt gaa gaa tac ggt tgg gaa 912

Gly Val Glu Tyr Ser Leu Lys Gln Met Val Glu Glu Tyr Gly Trp Glu

290 295 300

gat atg gaa gtt gtc tct gtt tct ggt aac tac tgt acc gac aag aag 960

Asp Met Glu Val Val Ser Val Ser Gly Asn Tyr Cys Thr Asp Lys Lys

305 310 315 320

cca gct gcc atc aac tgg atc gaa ggt cgt ggt aag tcc gtt gtt gct 1008

Pro Ala Ala Ile Asn Trp Ile Glu Gly Arg Gly Lys Ser Val Val Ala

325 330 335

gaa gct acc att cca ggt gac gtt gtc aga aag gtt ttg aaa tct gat 1056

Glu Ala Thr Ile Pro Gly Asp Val Val Arg Lys Val Leu Lys Ser Asp

340 345 350

gtt tct gct tta gtc gaa ttg aac att gcc aag aac ttg gtc ggt tct 1104

Val Ser Ala Leu Val Glu Leu Asn Ile Ala Lys Asn Leu Val Gly Ser

355 360 365

gcc atg gct ggt tcc gtc ggt ggt ttc aac gct cat gcc gct aac ttg 1152

Ala Met Ala Gly Ser Val Gly Gly Phe Asn Ala His Ala Ala Asn Leu

370 375 380

gtc act gct gtt ttc ttg gct tta ggt caa gat cca gct caa aat gtc 1200

Val Thr Ala Val Phe Leu Ala Leu Gly Gln Asp Pro Ala Gln Asn Val

385 390 395 400

gaa tcc tct aac tgt atc act ttg atg aag gaa gtt gac ggt gat ttg 1248

Glu Ser Ser Asn Cys Ile Thr Leu Met Lys Glu Val Asp Gly Asp Leu

405 410 415

aga att tct gtt tcc atg cca tcc att gaa gtc ggt act atc ggt ggt 1296

Arg Ile Ser Val Ser Met Pro Ser Ile Glu Val Gly Thr Ile Gly Gly

420 425 430

ggt act gtc ttg gaa cca caa ggt gcc atg ttg gac ttg ttg ggt gtt 1344

Gly Thr Val Leu Glu Pro Gln Gly Ala Met Leu Asp Leu Leu Gly Val

435 440 445

cgt ggt cca cac gct acc gct cca ggt act aac gcc aga caa ttg gcc 1392

Arg Gly Pro His Ala Thr Ala Pro Gly Thr Asn Ala Arg Gln Leu Ala

450 455 460

aga att gtt gcc tgt gcc gtc ttg gct ggt gaa ttg tct cta tgt gcc 1440

Arg Ile Val Ala Cys Ala Val Leu Ala Gly Glu Leu Ser Leu Cys Ala

465 470 475 480

gct ttg gct gct ggt cac ttg gtt caa tct cac atg acc cac aac aga 1488

Ala Leu Ala Ala Gly His Leu Val Gln Ser His Met Thr His Asn Arg

485 490 495

aag cct gct gaa cca acc aaa cca aac aac ttg gat gct act gac att 1536

Lys Pro Ala Glu Pro Thr Lys Pro Asn Asn Leu Asp Ala Thr Asp Ile

500 505 510

aac aga tta aag gac ggt tct gtc acc tgt atc aag tct 1575

Asn Arg Leu Lys Asp Gly Ser Val Thr Cys Ile Lys Ser

515 520 525

<210> 80

<211> 525

<212> PRT

<213> Saccharomyces cerevisiae

<400> 80

Met Asp Gln Leu Val Lys Thr Glu Val Thr Lys Lys Ser Phe Thr Ala

1 5 10 15

Pro Val Gln Lys Ala Ser Thr Pro Val Leu Thr Asn Lys Thr Val Ile

20 25 30

Ser Gly Ser Lys Val Lys Ser Leu Ser Ser Ala Gln Ser Ser Ser Ser

35 40 45

Gly Pro Ser Ser Ser Ser Glu Glu Asp Asp Ser Arg Asp Ile Glu Ser

50 55 60

Leu Asp Lys Lys Ile Arg Pro Leu Glu Glu Leu Glu Ala Leu Leu Ser

65 70 75 80

Ser Gly Asn Thr Lys Gln Leu Lys Asn Lys Glu Val Ala Ala Leu Val

85 90 95

Ile His Gly Lys Leu Pro Leu Tyr Ala Leu Glu Lys Lys Leu Gly Asp

100 105 110

Thr Thr Arg Ala Val Ala Val Arg Arg Lys Ala Leu Ser Ile Leu Ala

115 120 125

Glu Ala Pro Val Leu Ala Ser Asp Arg Leu Pro Tyr Lys Asn Tyr Asp

130 135 140

Tyr Asp Arg Val Phe Gly Ala Cys Cys Glu Asn Val Ile Gly Tyr Met

145 150 155 160

Pro Leu Pro Val Gly Val Ile Gly Pro Leu Val Ile Asp Gly Thr Ser

165 170 175

Tyr His Ile Pro Met Ala Thr Thr Glu Gly Cys Leu Val Ala Ser Ala

180 185 190

Met Arg Gly Cys Lys Ala Ile Asn Ala Gly Gly Gly Ala Thr Thr Val

195 200 205

Leu Thr Lys Asp Gly Met Thr Arg Gly Pro Val Val Arg Phe Pro Thr

210 215 220

Leu Lys Arg Ser Gly Ala Cys Lys Ile Trp Leu Asp Ser Glu Glu Gly

225 230 235 240

Gln Asn Ala Ile Lys Lys Ala Phe Asn Ser Thr Ser Arg Phe Ala Arg

245 250 255

Leu Gln His Ile Gln Thr Cys Leu Ala Gly Asp Leu Leu Phe Met Arg

260 265 270

Phe Arg Thr Thr Thr Gly Asp Ala Met Gly Met Asn Met Ile Ser Lys

275 280 285

Gly Val Glu Tyr Ser Leu Lys Gln Met Val Glu Glu Tyr Gly Trp Glu

290 295 300

Asp Met Glu Val Val Ser Val Ser Gly Asn Tyr Cys Thr Asp Lys Lys

305 310 315 320

Pro Ala Ala Ile Asn Trp Ile Glu Gly Arg Gly Lys Ser Val Val Ala

325 330 335

Glu Ala Thr Ile Pro Gly Asp Val Val Arg Lys Val Leu Lys Ser Asp

340 345 350

Val Ser Ala Leu Val Glu Leu Asn Ile Ala Lys Asn Leu Val Gly Ser

355 360 365

Ala Met Ala Gly Ser Val Gly Gly Phe Asn Ala His Ala Ala Asn Leu

370 375 380

Val Thr Ala Val Phe Leu Ala Leu Gly Gln Asp Pro Ala Gln Asn Val

385 390 395 400

Glu Ser Ser Asn Cys Ile Thr Leu Met Lys Glu Val Asp Gly Asp Leu

405 410 415

Arg Ile Ser Val Ser Met Pro Ser Ile Glu Val Gly Thr Ile Gly Gly

420 425 430

Gly Thr Val Leu Glu Pro Gln Gly Ala Met Leu Asp Leu Leu Gly Val

435 440 445

Arg Gly Pro His Ala Thr Ala Pro Gly Thr Asn Ala Arg Gln Leu Ala

450 455 460

Arg Ile Val Ala Cys Ala Val Leu Ala Gly Glu Leu Ser Leu Cys Ala

465 470 475 480

Ala Leu Ala Ala Gly His Leu Val Gln Ser His Met Thr His Asn Arg

485 490 495

Lys Pro Ala Glu Pro Thr Lys Pro Asn Asn Leu Asp Ala Thr Asp Ile

500 505 510

Asn Arg Leu Lys Asp Gly Ser Val Thr Cys Ile Lys Ser

515 520 525

<210> 81

<211> 1056

<212> DNA

<213> Saccharomyces cerevisiae

<220>

<221> CDS

<222> (1)..(1056)

<400> 81

atg gct tct gaa aag gaa atc aga aga gaa cgt ttc ttg aat gtt ttc 48

Met Ala Ser Glu Lys Glu Ile Arg Arg Glu Arg Phe Leu Asn Val Phe

1 5 10 15

cca aaa ttg gtt gaa gaa ttg aac gct tct cta tta gct tac ggt atg 96

Pro Lys Leu Val Glu Glu Leu Asn Ala Ser Leu Leu Ala Tyr Gly Met

20 25 30

cca aag gaa gct tgt gac tgg tac gct cac tct ttg aac tac aac acc 144

Pro Lys Glu Ala Cys Asp Trp Tyr Ala His Ser Leu Asn Tyr Asn Thr

35 40 45

cca ggt ggt aag ttg aac aga ggt cta tcc gtt gtt gac acc tac gcc 192

Pro Gly Gly Lys Leu Asn Arg Gly Leu Ser Val Val Asp Thr Tyr Ala

50 55 60

att ttg tcc aac aag acc gtc gaa caa tta ggt caa gaa gaa tac gaa 240

Ile Leu Ser Asn Lys Thr Val Glu Gln Leu Gly Gln Glu Glu Tyr Glu

65 70 75 80

aag gtt gcc atc tta ggt tgg tgt atc gaa ttg ttg caa gct tac ttc 288

Lys Val Ala Ile Leu Gly Trp Cys Ile Glu Leu Leu Gln Ala Tyr Phe

85 90 95

ttg gtt gct gat gac atg atg gac aaa tct atc acc aga aga ggt caa 336

Leu Val Ala Asp Asp Met Met Asp Lys Ser Ile Thr Arg Arg Gly Gln

100 105 110

cca tgt tgg tac aag gtt cca gaa gtc ggt gaa att gcc atc aac gat 384

Pro Cys Trp Tyr Lys Val Pro Glu Val Gly Glu Ile Ala Ile Asn Asp

115 120 125

gct ttc atg ttg gaa gct gcc atc tac aag ttg ttg aag tct cac ttc 432

Ala Phe Met Leu Glu Ala Ala Ile Tyr Lys Leu Leu Lys Ser His Phe

130 135 140

aga aac gaa aag tac tac att gac atc act gaa tta ttc cac gaa gtt 480

Arg Asn Glu Lys Tyr Tyr Ile Asp Ile Thr Glu Leu Phe His Glu Val

145 150 155 160

act ttc caa acc gaa ttg ggt caa ttg atg gac ttg att acc gct cca 528

Thr Phe Gln Thr Glu Leu Gly Gln Leu Met Asp Leu Ile Thr Ala Pro

165 170 175

gaa gat aag gtc gat ttg tcc aaa ttt tcc ttg aag aaa cac tct ttc 576

Glu Asp Lys Val Asp Leu Ser Lys Phe Ser Leu Lys Lys His Ser Phe

180 185 190

att gtc act ttc aag act gct tac tac tcc ttt tac ttg cct gtt gct 624

Ile Val Thr Phe Lys Thr Ala Tyr Tyr Ser Phe Tyr Leu Pro Val Ala

195 200 205

ttg gcc atg tat gtc gct ggt atc acc gat gaa aag gac ttg aag caa 672

Leu Ala Met Tyr Val Ala Gly Ile Thr Asp Glu Lys Asp Leu Lys Gln

210 215 220

gct cgt gat gtc ttg att cca tta ggt gaa tac ttc caa atc caa gat 720

Ala Arg Asp Val Leu Ile Pro Leu Gly Glu Tyr Phe Gln Ile Gln Asp

225 230 235 240

gac tac ttg gac tgt ttc ggt act cca gaa caa atc ggt aag att ggt 768

Asp Tyr Leu Asp Cys Phe Gly Thr Pro Glu Gln Ile Gly Lys Ile Gly

245 250 255

act gat atc caa gac aac aag tgt tcc tgg gtt atc aac aag gct ttg 816

Thr Asp Ile Gln Asp Asn Lys Cys Ser Trp Val Ile Asn Lys Ala Leu

260 265 270

gaa ttg gct tct gct gaa caa aga aag act ttg gac gaa aac tac ggt 864

Glu Leu Ala Ser Ala Glu Gln Arg Lys Thr Leu Asp Glu Asn Tyr Gly

275 280 285

aag aag gac tct gtt gct gaa gct aag tgt aag aag atc ttc aac gat 912

Lys Lys Asp Ser Val Ala Glu Ala Lys Cys Lys Lys Ile Phe Asn Asp

290 295 300

ttg aaa att gaa caa tta tac cat gaa tac gaa gaa tct att gcc aag 960

Leu Lys Ile Glu Gln Leu Tyr His Glu Tyr Glu Glu Ser Ile Ala Lys

305 310 315 320

gac ttg aaa gcc aag atc tct caa gtc gac gaa tcc aga ggt ttc aag 1008

Asp Leu Lys Ala Lys Ile Ser Gln Val Asp Glu Ser Arg Gly Phe Lys

325 330 335

gct gat gtc ttg act gct ttc ttg aac aag gtc tac aag aga tca aaa 1056

Ala Asp Val Leu Thr Ala Phe Leu Asn Lys Val Tyr Lys Arg Ser Lys

340 345 350

<210> 82

<211> 352

<212> PRT

<213> Saccharomyces cerevisiae

<400> 82

Met Ala Ser Glu Lys Glu Ile Arg Arg Glu Arg Phe Leu Asn Val Phe

1 5 10 15

Pro Lys Leu Val Glu Glu Leu Asn Ala Ser Leu Leu Ala Tyr Gly Met

20 25 30

Pro Lys Glu Ala Cys Asp Trp Tyr Ala His Ser Leu Asn Tyr Asn Thr

35 40 45

Pro Gly Gly Lys Leu Asn Arg Gly Leu Ser Val Val Asp Thr Tyr Ala

50 55 60

Ile Leu Ser Asn Lys Thr Val Glu Gln Leu Gly Gln Glu Glu Tyr Glu

65 70 75 80

Lys Val Ala Ile Leu Gly Trp Cys Ile Glu Leu Leu Gln Ala Tyr Phe

85 90 95

Leu Val Ala Asp Asp Met Met Asp Lys Ser Ile Thr Arg Arg Gly Gln

100 105 110

Pro Cys Trp Tyr Lys Val Pro Glu Val Gly Glu Ile Ala Ile Asn Asp

115 120 125

Ala Phe Met Leu Glu Ala Ala Ile Tyr Lys Leu Leu Lys Ser His Phe

130 135 140

Arg Asn Glu Lys Tyr Tyr Ile Asp Ile Thr Glu Leu Phe His Glu Val

145 150 155 160

Thr Phe Gln Thr Glu Leu Gly Gln Leu Met Asp Leu Ile Thr Ala Pro

165 170 175

Glu Asp Lys Val Asp Leu Ser Lys Phe Ser Leu Lys Lys His Ser Phe

180 185 190

Ile Val Thr Phe Lys Thr Ala Tyr Tyr Ser Phe Tyr Leu Pro Val Ala

195 200 205

Leu Ala Met Tyr Val Ala Gly Ile Thr Asp Glu Lys Asp Leu Lys Gln

210 215 220

Ala Arg Asp Val Leu Ile Pro Leu Gly Glu Tyr Phe Gln Ile Gln Asp

225 230 235 240

Asp Tyr Leu Asp Cys Phe Gly Thr Pro Glu Gln Ile Gly Lys Ile Gly

245 250 255

Thr Asp Ile Gln Asp Asn Lys Cys Ser Trp Val Ile Asn Lys Ala Leu

260 265 270

Glu Leu Ala Ser Ala Glu Gln Arg Lys Thr Leu Asp Glu Asn Tyr Gly

275 280 285

Lys Lys Asp Ser Val Ala Glu Ala Lys Cys Lys Lys Ile Phe Asn Asp

290 295 300

Leu Lys Ile Glu Gln Leu Tyr His Glu Tyr Glu Glu Ser Ile Ala Lys

305 310 315 320

Asp Leu Lys Ala Lys Ile Ser Gln Val Asp Glu Ser Arg Gly Phe Lys

325 330 335

Ala Asp Val Leu Thr Ala Phe Leu Asn Lys Val Tyr Lys Arg Ser Lys

340 345 350

<210> 83

<211> 1005

<212> DNA

<213> Saccharomyces cerevisiae

<220>

<221> CDS

<222> (1)..(1005)

<400> 83

atg gaa gct aag att gac gaa ttg atc aac aac gac cct gtc tgg tcc 48

Met Glu Ala Lys Ile Asp Glu Leu Ile Asn Asn Asp Pro Val Trp Ser

1 5 10 15

tct caa aac gaa tct ttg atc tcc aag cca tac aac cac atc ttg ttg 96

Ser Gln Asn Glu Ser Leu Ile Ser Lys Pro Tyr Asn His Ile Leu Leu

20 25 30

aag cca ggt aag aac ttc aga tta aac ttg att gtt caa atc aac aga 144

Lys Pro Gly Lys Asn Phe Arg Leu Asn Leu Ile Val Gln Ile Asn Arg

35 40 45

gtt atg aac ttg cca aag gac caa ttg gcc att gtt tcc caa att gtc 192

Val Met Asn Leu Pro Lys Asp Gln Leu Ala Ile Val Ser Gln Ile Val

50 55 60

gaa ttg ttg cac aac tcc tct cta ttg atc gat gac att gaa gat aat 240

Glu Leu Leu His Asn Ser Ser Leu Leu Ile Asp Asp Ile Glu Asp Asn

65 70 75 80

gct cca tta aga aga ggt caa acc act tct cat ttg att ttc ggt gtc 288

Ala Pro Leu Arg Arg Gly Gln Thr Thr Ser His Leu Ile Phe Gly Val

85 90 95

cca tcc acc atc aac act gct aac tac atg tac ttc aga gcc atg caa 336

Pro Ser Thr Ile Asn Thr Ala Asn Tyr Met Tyr Phe Arg Ala Met Gln

100 105 110

ttg gtt tct caa ttg acc acc aag gaa cca tta tac cac aac ttg atc 384

Leu Val Ser Gln Leu Thr Thr Lys Glu Pro Leu Tyr His Asn Leu Ile

115 120 125

act atc ttt aac gaa gaa ttg att aac ttg cac cgt ggt caa ggt ttg 432

Thr Ile Phe Asn Glu Glu Leu Ile Asn Leu His Arg Gly Gln Gly Leu

130 135 140

gac atc tac tgg aga gat ttc ttg cca gaa att att cca act caa gaa 480

Asp Ile Tyr Trp Arg Asp Phe Leu Pro Glu Ile Ile Pro Thr Gln Glu

145 150 155 160

atg tac ttg aac atg gtc atg aac aag act ggt ggt tta ttc aga ttg 528

Met Tyr Leu Asn Met Val Met Asn Lys Thr Gly Gly Leu Phe Arg Leu

165 170 175

act tta cgt ttg atg gaa gct ttg tct cca tct tcc cac cac ggt cac 576

Thr Leu Arg Leu Met Glu Ala Leu Ser Pro Ser Ser His His Gly His

180 185 190

tct ttg gtt cca ttc atc aat cta tta ggt atc atc tac caa atc aga 624

Ser Leu Val Pro Phe Ile Asn Leu Leu Gly Ile Ile Tyr Gln Ile Arg

195 200 205

gat gat tac ttg aac ttg aag gac ttc caa atg tcc tct gaa aag ggt 672

Asp Asp Tyr Leu Asn Leu Lys Asp Phe Gln Met Ser Ser Glu Lys Gly

210 215 220

ttc gct gaa gat atc act gaa ggt aaa ttg tct ttc cca att gtc cac 720

Phe Ala Glu Asp Ile Thr Glu Gly Lys Leu Ser Phe Pro Ile Val His

225 230 235 240

gcc ttg aac ttt acc aag acc aag ggt caa act gaa caa cac aac gaa 768

Ala Leu Asn Phe Thr Lys Thr Lys Gly Gln Thr Glu Gln His Asn Glu

245 250 255

att ttg aga atc tta ttg ttg aga act tct gac aag gac atc aag ttg 816

Ile Leu Arg Ile Leu Leu Leu Arg Thr Ser Asp Lys Asp Ile Lys Leu

260 265 270

aaa ttg atc caa atc ttg gaa ttc gat acc aac tct ttg gct tac acc 864

Lys Leu Ile Gln Ile Leu Glu Phe Asp Thr Asn Ser Leu Ala Tyr Thr

275 280 285

aag aac ttc atc aac caa ttg gtt aac atg atc aag aat gac aac gaa 912

Lys Asn Phe Ile Asn Gln Leu Val Asn Met Ile Lys Asn Asp Asn Glu

290 295 300

aac aaa tac ttg cca gac ttg gct tcc cac tcc gat acc gct acc aac 960

Asn Lys Tyr Leu Pro Asp Leu Ala Ser His Ser Asp Thr Ala Thr Asn

305 310 315 320

ttg cac gac gaa ttg ttg tac att att gac cat ttg tct gag tta 1005

Leu His Asp Glu Leu Leu Tyr Ile Ile Asp His Leu Ser Glu Leu

325 330 335

<210> 84

<211> 335

<212> PRT

<213> Saccharomyces cerevisiae

<400> 84

Met Glu Ala Lys Ile Asp Glu Leu Ile Asn Asn Asp Pro Val Trp Ser

1 5 10 15

Ser Gln Asn Glu Ser Leu Ile Ser Lys Pro Tyr Asn His Ile Leu Leu

20 25 30

Lys Pro Gly Lys Asn Phe Arg Leu Asn Leu Ile Val Gln Ile Asn Arg

35 40 45

Val Met Asn Leu Pro Lys Asp Gln Leu Ala Ile Val Ser Gln Ile Val

50 55 60

Glu Leu Leu His Asn Ser Ser Leu Leu Ile Asp Asp Ile Glu Asp Asn

65 70 75 80

Ala Pro Leu Arg Arg Gly Gln Thr Thr Ser His Leu Ile Phe Gly Val

85 90 95

Pro Ser Thr Ile Asn Thr Ala Asn Tyr Met Tyr Phe Arg Ala Met Gln

100 105 110

Leu Val Ser Gln Leu Thr Thr Lys Glu Pro Leu Tyr His Asn Leu Ile

115 120 125

Thr Ile Phe Asn Glu Glu Leu Ile Asn Leu His Arg Gly Gln Gly Leu

130 135 140

Asp Ile Tyr Trp Arg Asp Phe Leu Pro Glu Ile Ile Pro Thr Gln Glu

145 150 155 160

Met Tyr Leu Asn Met Val Met Asn Lys Thr Gly Gly Leu Phe Arg Leu

165 170 175

Thr Leu Arg Leu Met Glu Ala Leu Ser Pro Ser Ser His His Gly His

180 185 190

Ser Leu Val Pro Phe Ile Asn Leu Leu Gly Ile Ile Tyr Gln Ile Arg

195 200 205

Asp Asp Tyr Leu Asn Leu Lys Asp Phe Gln Met Ser Ser Glu Lys Gly

210 215 220

Phe Ala Glu Asp Ile Thr Glu Gly Lys Leu Ser Phe Pro Ile Val His

225 230 235 240

Ala Leu Asn Phe Thr Lys Thr Lys Gly Gln Thr Glu Gln His Asn Glu

245 250 255

Ile Leu Arg Ile Leu Leu Leu Arg Thr Ser Asp Lys Asp Ile Lys Leu

260 265 270

Lys Leu Ile Gln Ile Leu Glu Phe Asp Thr Asn Ser Leu Ala Tyr Thr

275 280 285

Lys Asn Phe Ile Asn Gln Leu Val Asn Met Ile Lys Asn Asp Asn Glu

290 295 300

Asn Lys Tyr Leu Pro Asp Leu Ala Ser His Ser Asp Thr Ala Thr Asn

305 310 315 320

Leu His Asp Glu Leu Leu Tyr Ile Ile Asp His Leu Ser Glu Leu

325 330 335

<210> 85

<211> 1575

<212> DNA

<213> Gibberella fujikuroi

<220>

<221> CDS

<222> (1)..(1575)

<400> 85

atg tcc aag tct aac tcc atg aac tcc act tct cac gaa act tta ttc 48

Met Ser Lys Ser Asn Ser Met Asn Ser Thr Ser His Glu Thr Leu Phe

1 5 10 15

caa caa ttg gtt ttg ggt ttg gac aga atg cca ttg atg gat gtc cac 96

Gln Gln Leu Val Leu Gly Leu Asp Arg Met Pro Leu Met Asp Val His

20 25 30

tgg ttg atc tac gtt gct ttc ggt gct tgg tta tgt tcc tac gtc att 144

Trp Leu Ile Tyr Val Ala Phe Gly Ala Trp Leu Cys Ser Tyr Val Ile

35 40 45

cac gtt ttg tcc tct tct tct acc gtc aag gtt cca gtt gtc ggt tac 192

His Val Leu Ser Ser Ser Ser Thr Val Lys Val Pro Val Val Gly Tyr

50 55 60

aga tcc gtt ttc gaa cca acc tgg tta ttg aga tta aga ttt gtc tgg 240

Arg Ser Val Phe Glu Pro Thr Trp Leu Leu Arg Leu Arg Phe Val Trp

65 70 75 80

gaa ggt ggt tcc att att ggt caa ggt tac aac aaa ttc aag gac tct 288

Glu Gly Gly Ser Ile Ile Gly Gln Gly Tyr Asn Lys Phe Lys Asp Ser

85 90 95

atc ttc caa gtc aga aag ttg ggt act gac att gtt atc atc cca cca 336

Ile Phe Gln Val Arg Lys Leu Gly Thr Asp Ile Val Ile Ile Pro Pro

100 105 110

aac tac atc gat gaa gtc aga aag ttg tcc caa gac aag acc aga tct 384

Asn Tyr Ile Asp Glu Val Arg Lys Leu Ser Gln Asp Lys Thr Arg Ser

115 120 125

gtt gaa cca ttc atc aac gat ttc gct ggt caa tac acc aga ggt atg 432

Val Glu Pro Phe Ile Asn Asp Phe Ala Gly Gln Tyr Thr Arg Gly Met

130 135 140

gtc ttt cta caa tct gat ttg caa aac cgt gtc atc caa caa aga ttg 480

Val Phe Leu Gln Ser Asp Leu Gln Asn Arg Val Ile Gln Gln Arg Leu

145 150 155 160

act cca aag ttg gtt tct ttg act aag gtc atg aag gaa gaa ttg gac 528

Thr Pro Lys Leu Val Ser Leu Thr Lys Val Met Lys Glu Glu Leu Asp

165 170 175

tac gct ttg acc aag gaa atg cca gac atg aag aac gac gaa tgg gtt 576

Tyr Ala Leu Thr Lys Glu Met Pro Asp Met Lys Asn Asp Glu Trp Val

180 185 190

gaa gtt gac att tct tcc atc atg gtc aga ttg atc tcc aga atc tct 624

Glu Val Asp Ile Ser Ser Ile Met Val Arg Leu Ile Ser Arg Ile Ser

195 200 205

gcc cgt gtt ttc ttg ggt cca gaa cac tgt cgt aac caa gaa tgg ttg 672

Ala Arg Val Phe Leu Gly Pro Glu His Cys Arg Asn Gln Glu Trp Leu

210 215 220

acc acc act gct gaa tac tct gaa tct tta ttc atc act ggt ttc atc 720

Thr Thr Thr Ala Glu Tyr Ser Glu Ser Leu Phe Ile Thr Gly Phe Ile

225 230 235 240

ttg aga gtt gtc cca cac atc tta aga cca ttc att gct cca ttg ttg 768

Leu Arg Val Val Pro His Ile Leu Arg Pro Phe Ile Ala Pro Leu Leu

245 250 255

cct tct tac aga act ttg ttg aga aat gtc tct tct ggt aga aga gtt 816

Pro Ser Tyr Arg Thr Leu Leu Arg Asn Val Ser Ser Gly Arg Arg Val

260 265 270

atc ggt gat atc atc aga tct caa caa ggt gat ggt aac gaa gat atc 864

Ile Gly Asp Ile Ile Arg Ser Gln Gln Gly Asp Gly Asn Glu Asp Ile

275 280 285

ttg tcc tgg atg aga gat gct gct acc ggt gaa gaa aag caa att gac 912

Leu Ser Trp Met Arg Asp Ala Ala Thr Gly Glu Glu Lys Gln Ile Asp

290 295 300

aac att gct caa aga atg ttg atc ttg tct ttg gct tcc att cac acc 960

Asn Ile Ala Gln Arg Met Leu Ile Leu Ser Leu Ala Ser Ile His Thr

305 310 315 320

acc gcc atg acc atg acc cat gcc atg tac gac ttg tgt gcc tgt cca 1008

Thr Ala Met Thr Met Thr His Ala Met Tyr Asp Leu Cys Ala Cys Pro

325 330 335

gaa tac att gaa cca tta cgt gac gaa gtc aaa tcc gtt gtt ggt gct 1056

Glu Tyr Ile Glu Pro Leu Arg Asp Glu Val Lys Ser Val Val Gly Ala

340 345 350

tct ggt tgg gac aag act gct ttg aac aga ttc cac aag ttg gac tct 1104

Ser Gly Trp Asp Lys Thr Ala Leu Asn Arg Phe His Lys Leu Asp Ser

355 360 365

ttc ttg aag gaa tct caa aga ttc aac cca gtt ttc ttg ttg act ttc 1152

Phe Leu Lys Glu Ser Gln Arg Phe Asn Pro Val Phe Leu Leu Thr Phe

370 375 380

aac aga atc tac cat caa tcc atg act ttg tcc gat ggt acc aac att 1200

Asn Arg Ile Tyr His Gln Ser Met Thr Leu Ser Asp Gly Thr Asn Ile

385 390 395 400

cca tct ggt acc aga att gct gtt cca tct cac gct atg ttg caa gat 1248

Pro Ser Gly Thr Arg Ile Ala Val Pro Ser His Ala Met Leu Gln Asp

405 410 415

tct gct cac gtt cca ggt cca act cct cca act gaa ttt gac ggt ttc 1296

Ser Ala His Val Pro Gly Pro Thr Pro Pro Thr Glu Phe Asp Gly Phe

420 425 430

aga tac tcc aag atc aga tct gac tct aac tat gct caa aag tac ttg 1344

Arg Tyr Ser Lys Ile Arg Ser Asp Ser Asn Tyr Ala Gln Lys Tyr Leu

435 440 445

ttc tcc atg acc gat tct tcc aac atg gct ttc ggt tac ggt aag tac 1392

Phe Ser Met Thr Asp Ser Ser Asn Met Ala Phe Gly Tyr Gly Lys Tyr

450 455 460

gct tgt cca ggt cgt ttc tac gcc tcc aac gaa atg aaa ttg act ttg 1440

Ala Cys Pro Gly Arg Phe Tyr Ala Ser Asn Glu Met Lys Leu Thr Leu

465 470 475 480

gcc att ttg ttg ttg caa ttt gaa ttc aaa ttg cca gat ggt aag ggt 1488

Ala Ile Leu Leu Leu Gln Phe Glu Phe Lys Leu Pro Asp Gly Lys Gly

485 490 495

aga cca aga aac atc act atc gac tct gac atg att cca gac cca aga 1536

Arg Pro Arg Asn Ile Thr Ile Asp Ser Asp Met Ile Pro Asp Pro Arg

500 505 510

gct aga tta tgt gtc aga aag aga tct cta cgt gac gaa 1575

Ala Arg Leu Cys Val Arg Lys Arg Ser Leu Arg Asp Glu

515 520 525

<210> 86

<211> 525

<212> PRT

<213> Gibberella fujikuroi

<400> 86

Met Ser Lys Ser Asn Ser Met Asn Ser Thr Ser His Glu Thr Leu Phe

1 5 10 15

Gln Gln Leu Val Leu Gly Leu Asp Arg Met Pro Leu Met Asp Val His

20 25 30

Trp Leu Ile Tyr Val Ala Phe Gly Ala Trp Leu Cys Ser Tyr Val Ile

35 40 45

His Val Leu Ser Ser Ser Ser Thr Val Lys Val Pro Val Val Gly Tyr

50 55 60

Arg Ser Val Phe Glu Pro Thr Trp Leu Leu Arg Leu Arg Phe Val Trp

65 70 75 80

Glu Gly Gly Ser Ile Ile Gly Gln Gly Tyr Asn Lys Phe Lys Asp Ser

85 90 95

Ile Phe Gln Val Arg Lys Leu Gly Thr Asp Ile Val Ile Ile Pro Pro

100 105 110

Asn Tyr Ile Asp Glu Val Arg Lys Leu Ser Gln Asp Lys Thr Arg Ser

115 120 125

Val Glu Pro Phe Ile Asn Asp Phe Ala Gly Gln Tyr Thr Arg Gly Met

130 135 140

Val Phe Leu Gln Ser Asp Leu Gln Asn Arg Val Ile Gln Gln Arg Leu

145 150 155 160

Thr Pro Lys Leu Val Ser Leu Thr Lys Val Met Lys Glu Glu Leu Asp

165 170 175

Tyr Ala Leu Thr Lys Glu Met Pro Asp Met Lys Asn Asp Glu Trp Val

180 185 190

Glu Val Asp Ile Ser Ser Ile Met Val Arg Leu Ile Ser Arg Ile Ser

195 200 205

Ala Arg Val Phe Leu Gly Pro Glu His Cys Arg Asn Gln Glu Trp Leu

210 215 220

Thr Thr Thr Ala Glu Tyr Ser Glu Ser Leu Phe Ile Thr Gly Phe Ile

225 230 235 240

Leu Arg Val Val Pro His Ile Leu Arg Pro Phe Ile Ala Pro Leu Leu

245 250 255

Pro Ser Tyr Arg Thr Leu Leu Arg Asn Val Ser Ser Gly Arg Arg Val

260 265 270

Ile Gly Asp Ile Ile Arg Ser Gln Gln Gly Asp Gly Asn Glu Asp Ile

275 280 285

Leu Ser Trp Met Arg Asp Ala Ala Thr Gly Glu Glu Lys Gln Ile Asp

290 295 300

Asn Ile Ala Gln Arg Met Leu Ile Leu Ser Leu Ala Ser Ile His Thr

305 310 315 320

Thr Ala Met Thr Met Thr His Ala Met Tyr Asp Leu Cys Ala Cys Pro

325 330 335

Glu Tyr Ile Glu Pro Leu Arg Asp Glu Val Lys Ser Val Val Gly Ala

340 345 350

Ser Gly Trp Asp Lys Thr Ala Leu Asn Arg Phe His Lys Leu Asp Ser

355 360 365

Phe Leu Lys Glu Ser Gln Arg Phe Asn Pro Val Phe Leu Leu Thr Phe

370 375 380

Asn Arg Ile Tyr His Gln Ser Met Thr Leu Ser Asp Gly Thr Asn Ile

385 390 395 400

Pro Ser Gly Thr Arg Ile Ala Val Pro Ser His Ala Met Leu Gln Asp

405 410 415

Ser Ala His Val Pro Gly Pro Thr Pro Pro Thr Glu Phe Asp Gly Phe

420 425 430

Arg Tyr Ser Lys Ile Arg Ser Asp Ser Asn Tyr Ala Gln Lys Tyr Leu

435 440 445

Phe Ser Met Thr Asp Ser Ser Asn Met Ala Phe Gly Tyr Gly Lys Tyr

450 455 460

Ala Cys Pro Gly Arg Phe Tyr Ala Ser Asn Glu Met Lys Leu Thr Leu

465 470 475 480

Ala Ile Leu Leu Leu Gln Phe Glu Phe Lys Leu Pro Asp Gly Lys Gly

485 490 495

Arg Pro Arg Asn Ile Thr Ile Asp Ser Asp Met Ile Pro Asp Pro Arg

500 505 510

Ala Arg Leu Cys Val Arg Lys Arg Ser Leu Arg Asp Glu

515 520 525

<210> 87

<211> 1419

<212> DNA

<213> Stevia rebaudiana

<220>

<221> CDS

<222> (1)..(1419)

<400> 87

atg gcc act tct gac tcc atc gtt gat gac aga aag caa ttg cac gtt 48

Met Ala Thr Ser Asp Ser Ile Val Asp Asp Arg Lys Gln Leu His Val

1 5 10 15

gct act ttc cca tgg tta gct ttc ggt cac att ttg cca tac ttg caa 96

Ala Thr Phe Pro Trp Leu Ala Phe Gly His Ile Leu Pro Tyr Leu Gln

20 25 30

ttg tcc aaa ttg att gct gaa aag ggt cac aaa gtc tct ttc ttg tcc 144

Leu Ser Lys Leu Ile Ala Glu Lys Gly His Lys Val Ser Phe Leu Ser

35 40 45

acc acc aga aac atc caa aga tta tct tct cac att tct cca ttg atc 192

Thr Thr Arg Asn Ile Gln Arg Leu Ser Ser His Ile Ser Pro Leu Ile

50 55 60

aac gtt gtc caa ttg act tta cca aga gtt caa gaa ttg cca gaa gat 240

Asn Val Val Gln Leu Thr Leu Pro Arg Val Gln Glu Leu Pro Glu Asp

65 70 75 80

gct gaa gct acc acc gat gtc cat cca gaa gat atc cca tac ttg aag 288

Ala Glu Ala Thr Thr Asp Val His Pro Glu Asp Ile Pro Tyr Leu Lys

85 90 95

aag gct tct gat ggt ttg caa cca gaa gtc act aga ttc ttg gaa caa 336

Lys Ala Ser Asp Gly Leu Gln Pro Glu Val Thr Arg Phe Leu Glu Gln

100 105 110

cac tct cca gac tgg att atc tac gac tac act cac tac tgg tta cca 384

His Ser Pro Asp Trp Ile Ile Tyr Asp Tyr Thr His Tyr Trp Leu Pro

115 120 125

tcc att gct gcc tct ttg ggt atc tct cgt gct cat ttc tcc gtt acc 432

Ser Ile Ala Ala Ser Leu Gly Ile Ser Arg Ala His Phe Ser Val Thr

130 135 140

act cca tgg gct att gct tac atg ggt cca tct gct gat gct atg atc 480

Thr Pro Trp Ala Ile Ala Tyr Met Gly Pro Ser Ala Asp Ala Met Ile

145 150 155 160

aac ggt tct gat ggt aga acc act gtc gaa gac ttg acc act cca cca 528

Asn Gly Ser Asp Gly Arg Thr Thr Val Glu Asp Leu Thr Thr Pro Pro

165 170 175

aaa tgg ttc cca ttc cca acc aag gtc tgt tgg aga aag cac gat ttg 576

Lys Trp Phe Pro Phe Pro Thr Lys Val Cys Trp Arg Lys His Asp Leu

180 185 190

gcc aga tta gtt cca tac aag gcc cca ggt atc tct gac ggt tac aga 624

Ala Arg Leu Val Pro Tyr Lys Ala Pro Gly Ile Ser Asp Gly Tyr Arg

195 200 205

atg ggt tta gtc ttg aag ggt tct gac tgt ttg ttg tcc aag tgt tac 672

Met Gly Leu Val Leu Lys Gly Ser Asp Cys Leu Leu Ser Lys Cys Tyr

210 215 220

cat gaa ttc ggt act caa tgg tta cct ttg ttg gaa act ttg cac caa 720

His Glu Phe Gly Thr Gln Trp Leu Pro Leu Leu Glu Thr Leu His Gln

225 230 235 240

gtc cca gtt gtt cca gtt ggt ttg ttg cct cct gaa atc cca ggt gac 768

Val Pro Val Val Pro Val Gly Leu Leu Pro Pro Glu Ile Pro Gly Asp

245 250 255

gaa aag gac gaa acc tgg gtt tcc atc aag aaa tgg tta gat ggt aag 816

Glu Lys Asp Glu Thr Trp Val Ser Ile Lys Lys Trp Leu Asp Gly Lys

260 265 270

caa aag ggt tcc gtt gtc tac gtt gct ttg ggt tct gaa gtc ttg gtt 864

Gln Lys Gly Ser Val Val Tyr Val Ala Leu Gly Ser Glu Val Leu Val

275 280 285

tct caa act gaa gtt gtt gaa ttg gct ttg ggt ttg gaa ttg tcc ggt 912

Ser Gln Thr Glu Val Val Glu Leu Ala Leu Gly Leu Glu Leu Ser Gly

290 295 300

cta cca ttt gtc tgg gct tac aga aag cca aag ggt cca gct aag tct 960

Leu Pro Phe Val Trp Ala Tyr Arg Lys Pro Lys Gly Pro Ala Lys Ser

305 310 315 320

gac tct gtt gaa ttg cca gat ggt ttc gtc gaa aga acc aga gac aga 1008

Asp Ser Val Glu Leu Pro Asp Gly Phe Val Glu Arg Thr Arg Asp Arg

325 330 335

ggt ttg gtc tgg act tcc tgg gct cca caa ttg aga att ttg tcc cac 1056

Gly Leu Val Trp Thr Ser Trp Ala Pro Gln Leu Arg Ile Leu Ser His

340 345 350

gaa tct gtt tgt ggt ttc ttg acc cac tgt ggt tct ggt tcc att gtc 1104

Glu Ser Val Cys Gly Phe Leu Thr His Cys Gly Ser Gly Ser Ile Val

355 360 365

gaa ggt ttg atg ttc ggt cac cca ttg atc atg ttg cca atc ttc ggt 1152

Glu Gly Leu Met Phe Gly His Pro Leu Ile Met Leu Pro Ile Phe Gly

370 375 380

gac caa cca ttg aac gct aga tta ttg gaa gac aag caa gtc ggt att 1200

Asp Gln Pro Leu Asn Ala Arg Leu Leu Glu Asp Lys Gln Val Gly Ile

385 390 395 400

gaa atc cca aga aac gaa gaa gac ggt tgt ttg acc aag gaa tct gtt 1248

Glu Ile Pro Arg Asn Glu Glu Asp Gly Cys Leu Thr Lys Glu Ser Val

405 410 415

gcc cgt tct cta aga tct gtt gtt gtc gaa aag gaa ggt gaa atc tac 1296

Ala Arg Ser Leu Arg Ser Val Val Val Glu Lys Glu Gly Glu Ile Tyr

420 425 430

aag gct aac gct aga gaa ttg tcc aag atc tac aac gat acc aag gtc 1344

Lys Ala Asn Ala Arg Glu Leu Ser Lys Ile Tyr Asn Asp Thr Lys Val

435 440 445

gaa aag gaa tat gtc tcc caa ttt gtt gac tac ttg gaa aag aac gct 1392

Glu Lys Glu Tyr Val Ser Gln Phe Val Asp Tyr Leu Glu Lys Asn Ala

450 455 460

cgt gcc gtt gcc att gac cac gaa agc 1419

Arg Ala Val Ala Ile Asp His Glu Ser

465 470

<210> 88

<211> 473

<212> PRT

<213> Stevia rebaudiana

<400> 88

Met Ala Thr Ser Asp Ser Ile Val Asp Asp Arg Lys Gln Leu His Val

1 5 10 15

Ala Thr Phe Pro Trp Leu Ala Phe Gly His Ile Leu Pro Tyr Leu Gln

20 25 30

Leu Ser Lys Leu Ile Ala Glu Lys Gly His Lys Val Ser Phe Leu Ser

35 40 45

Thr Thr Arg Asn Ile Gln Arg Leu Ser Ser His Ile Ser Pro Leu Ile

50 55 60

Asn Val Val Gln Leu Thr Leu Pro Arg Val Gln Glu Leu Pro Glu Asp

65 70 75 80

Ala Glu Ala Thr Thr Asp Val His Pro Glu Asp Ile Pro Tyr Leu Lys

85 90 95

Lys Ala Ser Asp Gly Leu Gln Pro Glu Val Thr Arg Phe Leu Glu Gln

100 105 110

His Ser Pro Asp Trp Ile Ile Tyr Asp Tyr Thr His Tyr Trp Leu Pro

115 120 125

Ser Ile Ala Ala Ser Leu Gly Ile Ser Arg Ala His Phe Ser Val Thr

130 135 140

Thr Pro Trp Ala Ile Ala Tyr Met Gly Pro Ser Ala Asp Ala Met Ile

145 150 155 160

Asn Gly Ser Asp Gly Arg Thr Thr Val Glu Asp Leu Thr Thr Pro Pro

165 170 175

Lys Trp Phe Pro Phe Pro Thr Lys Val Cys Trp Arg Lys His Asp Leu

180 185 190

Ala Arg Leu Val Pro Tyr Lys Ala Pro Gly Ile Ser Asp Gly Tyr Arg

195 200 205

Met Gly Leu Val Leu Lys Gly Ser Asp Cys Leu Leu Ser Lys Cys Tyr

210 215 220

His Glu Phe Gly Thr Gln Trp Leu Pro Leu Leu Glu Thr Leu His Gln

225 230 235 240

Val Pro Val Val Pro Val Gly Leu Leu Pro Pro Glu Ile Pro Gly Asp

245 250 255

Glu Lys Asp Glu Thr Trp Val Ser Ile Lys Lys Trp Leu Asp Gly Lys

260 265 270

Gln Lys Gly Ser Val Val Tyr Val Ala Leu Gly Ser Glu Val Leu Val

275 280 285

Ser Gln Thr Glu Val Val Glu Leu Ala Leu Gly Leu Glu Leu Ser Gly

290 295 300

Leu Pro Phe Val Trp Ala Tyr Arg Lys Pro Lys Gly Pro Ala Lys Ser

305 310 315 320

Asp Ser Val Glu Leu Pro Asp Gly Phe Val Glu Arg Thr Arg Asp Arg

325 330 335

Gly Leu Val Trp Thr Ser Trp Ala Pro Gln Leu Arg Ile Leu Ser His

340 345 350

Glu Ser Val Cys Gly Phe Leu Thr His Cys Gly Ser Gly Ser Ile Val

355 360 365

Glu Gly Leu Met Phe Gly His Pro Leu Ile Met Leu Pro Ile Phe Gly

370 375 380

Asp Gln Pro Leu Asn Ala Arg Leu Leu Glu Asp Lys Gln Val Gly Ile

385 390 395 400

Glu Ile Pro Arg Asn Glu Glu Asp Gly Cys Leu Thr Lys Glu Ser Val

405 410 415

Ala Arg Ser Leu Arg Ser Val Val Val Glu Lys Glu Gly Glu Ile Tyr

420 425 430

Lys Ala Asn Ala Arg Glu Leu Ser Lys Ile Tyr Asn Asp Thr Lys Val

435 440 445

Glu Lys Glu Tyr Val Ser Gln Phe Val Asp Tyr Leu Glu Lys Asn Ala

450 455 460

Arg Ala Val Ala Ile Asp His Glu Ser

465 470

<210> 89

<211> 1575

<212> DNA

<213> Stevia rebaudiana

<220>

<221> CDS

<222> (1)..(1575)

<400> 89

atg ggt ttg ttc cca tta gag gat tcc tac gcg ctg gtc ttt gaa gga 48

Met Gly Leu Phe Pro Leu Glu Asp Ser Tyr Ala Leu Val Phe Glu Gly

1 5 10 15

cta gca ata aca ctg gct ttg tac tat cta ctg tct ttc atc tac aaa 96

Leu Ala Ile Thr Leu Ala Leu Tyr Tyr Leu Leu Ser Phe Ile Tyr Lys

20 25 30

aca tct aaa aag aca tgt aca cct cct aaa gca tct ggt gaa atc att 144

Thr Ser Lys Lys Thr Cys Thr Pro Pro Lys Ala Ser Gly Glu Ile Ile

35 40 45

cca att aca gga atc ata ttg aat ctg cta tct ggc tca agt ggt cta 192

Pro Ile Thr Gly Ile Ile Leu Asn Leu Leu Ser Gly Ser Ser Gly Leu

50 55 60

cct att atc tta gca ctt gcc tct tta gca gac aga tgt ggt cct att 240

Pro Ile Ile Leu Ala Leu Ala Ser Leu Ala Asp Arg Cys Gly Pro Ile

65 70 75 80

ttc acc att agg ctg ggt att agg aga gtg cta gta gta tca aat tgg 288

Phe Thr Ile Arg Leu Gly Ile Arg Arg Val Leu Val Val Ser Asn Trp

85 90 95

gaa atc gct aag gag att ttc act acc cac gat ttg ata gtt tct aat 336

Glu Ile Ala Lys Glu Ile Phe Thr Thr His Asp Leu Ile Val Ser Asn

100 105 110

aga cca aaa tac tta gcc gct aag att ctt ggt ttc aat tat gtt tca 384

Arg Pro Lys Tyr Leu Ala Ala Lys Ile Leu Gly Phe Asn Tyr Val Ser

115 120 125

ttc tct ttc gct cca tac ggc cca tat tgg gtc gga atc aga aag att 432

Phe Ser Phe Ala Pro Tyr Gly Pro Tyr Trp Val Gly Ile Arg Lys Ile

130 135 140

att gct aca aaa cta atg tct tct tcc aga ctt cag aag ttg caa ttt 480

Ile Ala Thr Lys Leu Met Ser Ser Ser Arg Leu Gln Lys Leu Gln Phe

145 150 155 160

gta aga gtt ttt gaa cta gaa aac tct atg aaa tct atc aga gaa tca 528

Val Arg Val Phe Glu Leu Glu Asn Ser Met Lys Ser Ile Arg Glu Ser

165 170 175

tgg aag gag aaa aag gat gaa gag gga aag gta tta gtt gag atg aaa 576

Trp Lys Glu Lys Lys Asp Glu Glu Gly Lys Val Leu Val Glu Met Lys

180 185 190

aag tgg ttc tgg gaa ctg aat atg aac ata gtg tta agg aca gtt gct 624

Lys Trp Phe Trp Glu Leu Asn Met Asn Ile Val Leu Arg Thr Val Ala

195 200 205

ggt aaa caa tac act ggt aca gtt gat gat gcc gat gca aag cgt atc 672

Gly Lys Gln Tyr Thr Gly Thr Val Asp Asp Ala Asp Ala Lys Arg Ile

210 215 220

tcc gag tta ttc aga gaa tgg ttt cac tac act ggc aga ttt gtc gtt 720

Ser Glu Leu Phe Arg Glu Trp Phe His Tyr Thr Gly Arg Phe Val Val

225 230 235 240

gga gat gct ttt cct ttt cta ggt tgg ttg gac ctg ggc gga tac aaa 768

Gly Asp Ala Phe Pro Phe Leu Gly Trp Leu Asp Leu Gly Gly Tyr Lys

245 250 255

aag aca atg gaa tta gtt gct agt aga ttg gac tca atg gtc agt aaa 816

Lys Thr Met Glu Leu Val Ala Ser Arg Leu Asp Ser Met Val Ser Lys

260 265 270

tgg tta gat gag cat cgt aaa aag caa gct aac gat gac aaa aag gag 864

Trp Leu Asp Glu His Arg Lys Lys Gln Ala Asn Asp Asp Lys Lys Glu

275 280 285

gat atg gat ttc atg gat atc atg atc tcc atg aca gaa gca aat tca 912

Asp Met Asp Phe Met Asp Ile Met Ile Ser Met Thr Glu Ala Asn Ser

290 295 300

cca ctt gaa gga tac ggc act gat act att atc aag acc aca tgt atg 960

Pro Leu Glu Gly Tyr Gly Thr Asp Thr Ile Ile Lys Thr Thr Cys Met

305 310 315 320

act ttg att gtt tca gga gtt gat aca acc tca atc gta ctt act tgg 1008

Thr Leu Ile Val Ser Gly Val Asp Thr Thr Ser Ile Val Leu Thr Trp

325 330 335

gcc tta tca ctt ttg tta aac aac aga gat act ttg aaa aag gca caa 1056

Ala Leu Ser Leu Leu Leu Asn Asn Arg Asp Thr Leu Lys Lys Ala Gln

340 345 350

gag gaa tta gat atg tgc gta ggt aaa gga aga caa gtc aac gag tct 1104

Glu Glu Leu Asp Met Cys Val Gly Lys Gly Arg Gln Val Asn Glu Ser

355 360 365

gat ctt gtt aac ttg ata tac ttg gaa gca gtg ctt aaa gag gct tta 1152

Asp Leu Val Asn Leu Ile Tyr Leu Glu Ala Val Leu Lys Glu Ala Leu

370 375 380

aga ctt tac cca gca gcg ttc tta ggc gga cca aga gca ttc ttg gaa 1200

Arg Leu Tyr Pro Ala Ala Phe Leu Gly Gly Pro Arg Ala Phe Leu Glu

385 390 395 400

gat tgt act gtt gct ggt tat aga att cca aag ggc acc tgc ttg ttg 1248

Asp Cys Thr Val Ala Gly Tyr Arg Ile Pro Lys Gly Thr Cys Leu Leu

405 410 415

att aac atg tgg aaa ctg cat aga gat cca aac att tgg agt gat cct 1296

Ile Asn Met Trp Lys Leu His Arg Asp Pro Asn Ile Trp Ser Asp Pro

420 425 430

tgc gaa ttc aag cca gaa aga ttt ttg aca cct aat caa aag gat gtt 1344

Cys Glu Phe Lys Pro Glu Arg Phe Leu Thr Pro Asn Gln Lys Asp Val

435 440 445

gat gtg atc ggt atg gat ttc gaa ttg ata cca ttt ggt gcc ggc aga 1392

Asp Val Ile Gly Met Asp Phe Glu Leu Ile Pro Phe Gly Ala Gly Arg

450 455 460

aga tat tgt cca ggt act aga ttg gct tta cag atg ttg cat atc gta 1440

Arg Tyr Cys Pro Gly Thr Arg Leu Ala Leu Gln Met Leu His Ile Val

465 470 475 480

tta gcg aca ttg ctg caa aac ttc gaa atg tca aca cca aac gat gcg 1488

Leu Ala Thr Leu Leu Gln Asn Phe Glu Met Ser Thr Pro Asn Asp Ala

485 490 495

cca gtc gat atg act gct tct gtt ggc atg aca aat gcc aaa gca tca 1536

Pro Val Asp Met Thr Ala Ser Val Gly Met Thr Asn Ala Lys Ala Ser

500 505 510

cct tta gaa gtc ttg cta tca cct cgt gtt aaa tgg tcc 1575

Pro Leu Glu Val Leu Leu Ser Pro Arg Val Lys Trp Ser

515 520 525

<210> 90

<211> 525

<212> PRT

<213> Stevia rebaudiana

<400> 90

Met Gly Leu Phe Pro Leu Glu Asp Ser Tyr Ala Leu Val Phe Glu Gly

1 5 10 15

Leu Ala Ile Thr Leu Ala Leu Tyr Tyr Leu Leu Ser Phe Ile Tyr Lys

20 25 30

Thr Ser Lys Lys Thr Cys Thr Pro Pro Lys Ala Ser Gly Glu Ile Ile

35 40 45

Pro Ile Thr Gly Ile Ile Leu Asn Leu Leu Ser Gly Ser Ser Gly Leu

50 55 60

Pro Ile Ile Leu Ala Leu Ala Ser Leu Ala Asp Arg Cys Gly Pro Ile

65 70 75 80

Phe Thr Ile Arg Leu Gly Ile Arg Arg Val Leu Val Val Ser Asn Trp

85 90 95

Glu Ile Ala Lys Glu Ile Phe Thr Thr His Asp Leu Ile Val Ser Asn

100 105 110

Arg Pro Lys Tyr Leu Ala Ala Lys Ile Leu Gly Phe Asn Tyr Val Ser

115 120 125

Phe Ser Phe Ala Pro Tyr Gly Pro Tyr Trp Val Gly Ile Arg Lys Ile

130 135 140

Ile Ala Thr Lys Leu Met Ser Ser Ser Arg Leu Gln Lys Leu Gln Phe

145 150 155 160

Val Arg Val Phe Glu Leu Glu Asn Ser Met Lys Ser Ile Arg Glu Ser

165 170 175

Trp Lys Glu Lys Lys Asp Glu Glu Gly Lys Val Leu Val Glu Met Lys

180 185 190

Lys Trp Phe Trp Glu Leu Asn Met Asn Ile Val Leu Arg Thr Val Ala

195 200 205

Gly Lys Gln Tyr Thr Gly Thr Val Asp Asp Ala Asp Ala Lys Arg Ile

210 215 220

Ser Glu Leu Phe Arg Glu Trp Phe His Tyr Thr Gly Arg Phe Val Val

225 230 235 240

Gly Asp Ala Phe Pro Phe Leu Gly Trp Leu Asp Leu Gly Gly Tyr Lys

245 250 255

Lys Thr Met Glu Leu Val Ala Ser Arg Leu Asp Ser Met Val Ser Lys

260 265 270

Trp Leu Asp Glu His Arg Lys Lys Gln Ala Asn Asp Asp Lys Lys Glu

275 280 285

Asp Met Asp Phe Met Asp Ile Met Ile Ser Met Thr Glu Ala Asn Ser

290 295 300

Pro Leu Glu Gly Tyr Gly Thr Asp Thr Ile Ile Lys Thr Thr Cys Met

305 310 315 320

Thr Leu Ile Val Ser Gly Val Asp Thr Thr Ser Ile Val Leu Thr Trp

325 330 335

Ala Leu Ser Leu Leu Leu Asn Asn Arg Asp Thr Leu Lys Lys Ala Gln

340 345 350

Glu Glu Leu Asp Met Cys Val Gly Lys Gly Arg Gln Val Asn Glu Ser

355 360 365

Asp Leu Val Asn Leu Ile Tyr Leu Glu Ala Val Leu Lys Glu Ala Leu

370 375 380

Arg Leu Tyr Pro Ala Ala Phe Leu Gly Gly Pro Arg Ala Phe Leu Glu

385 390 395 400

Asp Cys Thr Val Ala Gly Tyr Arg Ile Pro Lys Gly Thr Cys Leu Leu

405 410 415

Ile Asn Met Trp Lys Leu His Arg Asp Pro Asn Ile Trp Ser Asp Pro

420 425 430

Cys Glu Phe Lys Pro Glu Arg Phe Leu Thr Pro Asn Gln Lys Asp Val

435 440 445

Asp Val Ile Gly Met Asp Phe Glu Leu Ile Pro Phe Gly Ala Gly Arg

450 455 460

Arg Tyr Cys Pro Gly Thr Arg Leu Ala Leu Gln Met Leu His Ile Val

465 470 475 480

Leu Ala Thr Leu Leu Gln Asn Phe Glu Met Ser Thr Pro Asn Asp Ala

485 490 495

Pro Val Asp Met Thr Ala Ser Val Gly Met Thr Asn Ala Lys Ala Ser

500 505 510

Pro Leu Glu Val Leu Leu Ser Pro Arg Val Lys Trp Ser

515 520 525

<210> 91

<211> 1428

<212> DNA

<213> Stevia rebaudiana

<220>

<221> CDS

<222> (1)..(1428)

<400> 91

atg ata caa gtt tta act cca att cta ctc ttc ctc atc ttc ttc gtt 48

Met Ile Gln Val Leu Thr Pro Ile Leu Leu Phe Leu Ile Phe Phe Val

1 5 10 15

ttc tgg aaa gtc tac aaa cat caa aag act aaa atc aat cta cca cca 96

Phe Trp Lys Val Tyr Lys His Gln Lys Thr Lys Ile Asn Leu Pro Pro

20 25 30

ggt tcc ttc ggc tgg cca ttt ttg ggt gaa acc tta gcc tta ctt aga 144

Gly Ser Phe Gly Trp Pro Phe Leu Gly Glu Thr Leu Ala Leu Leu Arg

35 40 45

gca ggc tgg gat tct gag cca gaa aga ttc gta aga gag cgt atc aaa 192

Ala Gly Trp Asp Ser Glu Pro Glu Arg Phe Val Arg Glu Arg Ile Lys

50 55 60

aag cat gga tct cca ctt gtt ttc aag aca tca cta ttt gga gac aga 240

Lys His Gly Ser Pro Leu Val Phe Lys Thr Ser Leu Phe Gly Asp Arg

65 70 75 80

ttc gct gtt ctt tgc ggt cca gct ggt aat aag ttt ttg ttc tgc aac 288

Phe Ala Val Leu Cys Gly Pro Ala Gly Asn Lys Phe Leu Phe Cys Asn

85 90 95

gaa aac aaa tta gtg gca tct tgg tgg cca gtc cct gta agg aag ttg 336

Glu Asn Lys Leu Val Ala Ser Trp Trp Pro Val Pro Val Arg Lys Leu

100 105 110

ttc ggt aaa agt tta ctc aca ata aga gga gat gaa gca aaa tgg atg 384

Phe Gly Lys Ser Leu Leu Thr Ile Arg Gly Asp Glu Ala Lys Trp Met

115 120 125

aga aaa atg cta ttg tct tac ttg ggt cca gat gca ttt gcc aca cat 432

Arg Lys Met Leu Leu Ser Tyr Leu Gly Pro Asp Ala Phe Ala Thr His

130 135 140

tat gcc gtt act atg gat gtt gta aca cgt aga cat att gat gtc cat 480

Tyr Ala Val Thr Met Asp Val Val Thr Arg Arg His Ile Asp Val His

145 150 155 160

tgg agg ggc aag gag gaa gtt aat gta ttt caa aca gtt aag ttg tac 528

Trp Arg Gly Lys Glu Glu Val Asn Val Phe Gln Thr Val Lys Leu Tyr

165 170 175

gca ttc gaa tta gct tgt aga tta ttc atg aac cta gat gac cca aac 576

Ala Phe Glu Leu Ala Cys Arg Leu Phe Met Asn Leu Asp Asp Pro Asn

180 185 190

cac atc gcg aaa ctc ggt agt ctt ttc aac att ttc ctc aaa ggg atc 624

His Ile Ala Lys Leu Gly Ser Leu Phe Asn Ile Phe Leu Lys Gly Ile

195 200 205

atc gag ctt cct ata gac gtt cct gga act aga ttt tac tcc agt aaa 672

Ile Glu Leu Pro Ile Asp Val Pro Gly Thr Arg Phe Tyr Ser Ser Lys

210 215 220

aag gcc gca gct gcc att aga att gaa ttg aaa aag ctc att aaa gct 720

Lys Ala Ala Ala Ala Ile Arg Ile Glu Leu Lys Lys Leu Ile Lys Ala

225 230 235 240

aga aaa ctc gaa ttg aag gag ggt aag gcg tct tct tca cag gac ttg 768

Arg Lys Leu Glu Leu Lys Glu Gly Lys Ala Ser Ser Ser Gln Asp Leu

245 250 255

ctt tct cat cta tta aca tca cct gat gag aat ggg atg ttc ttg aca 816

Leu Ser His Leu Leu Thr Ser Pro Asp Glu Asn Gly Met Phe Leu Thr

260 265 270

gaa gag gaa ata gtc gat aac att cta ctt ttg tta ttc gct ggt cac 864

Glu Glu Glu Ile Val Asp Asn Ile Leu Leu Leu Leu Phe Ala Gly His

275 280 285

gat acc tct gca cta tca ata aca ctt ttg atg aaa acc tta ggt gaa 912

Asp Thr Ser Ala Leu Ser Ile Thr Leu Leu Met Lys Thr Leu Gly Glu

290 295 300

cac agt gat gtg tac gac aag gtt ttg aag gaa caa tta gaa att tcc 960

His Ser Asp Val Tyr Asp Lys Val Leu Lys Glu Gln Leu Glu Ile Ser

305 310 315 320

aaa aca aag gag gct tgg gaa tca cta aag tgg gaa gat atc cag aag 1008

Lys Thr Lys Glu Ala Trp Glu Ser Leu Lys Trp Glu Asp Ile Gln Lys

325 330 335

atg aag tac tca tgg tca gta atc tgt gaa gtc atg aga ttg aat cct 1056

Met Lys Tyr Ser Trp Ser Val Ile Cys Glu Val Met Arg Leu Asn Pro

340 345 350

cct gtc ata ggg aca tac aga gag gcg ttg gtt gat atc gac tat gct 1104

Pro Val Ile Gly Thr Tyr Arg Glu Ala Leu Val Asp Ile Asp Tyr Ala

355 360 365

ggt tac act atc cca aaa gga tgg aag ttg cat tgg tca gct gtt tct 1152

Gly Tyr Thr Ile Pro Lys Gly Trp Lys Leu His Trp Ser Ala Val Ser

370 375 380

act caa aga gat gaa gcc aat ttc gaa gat gta act aga ttc gat cca 1200

Thr Gln Arg Asp Glu Ala Asn Phe Glu Asp Val Thr Arg Phe Asp Pro

385 390 395 400

tcc aga ttt gaa ggg gca ggc cct act cca ttc aca ttt gtg cct ttc 1248

Ser Arg Phe Glu Gly Ala Gly Pro Thr Pro Phe Thr Phe Val Pro Phe

405 410 415

ggt gga ggt cct aga atg tgt tta ggc aaa gag ttt gcc agg tta gaa 1296

Gly Gly Gly Pro Arg Met Cys Leu Gly Lys Glu Phe Ala Arg Leu Glu

420 425 430

gtg tta gca ttt ctc cac aac att gtt acc aac ttt aag tgg gat ctt 1344

Val Leu Ala Phe Leu His Asn Ile Val Thr Asn Phe Lys Trp Asp Leu

435 440 445

cta atc cct gat gag aag atc gaa tat gat cca atg gct act cca gct 1392

Leu Ile Pro Asp Glu Lys Ile Glu Tyr Asp Pro Met Ala Thr Pro Ala

450 455 460

aag ggc ttg cca att aga ctt cat cca cac caa gtc 1428

Lys Gly Leu Pro Ile Arg Leu His Pro His Gln Val

465 470 475

<210> 92

<211> 476

<212> PRT

<213> Stevia rebaudiana

<400> 92

Met Ile Gln Val Leu Thr Pro Ile Leu Leu Phe Leu Ile Phe Phe Val

1 5 10 15

Phe Trp Lys Val Tyr Lys His Gln Lys Thr Lys Ile Asn Leu Pro Pro

20 25 30

Gly Ser Phe Gly Trp Pro Phe Leu Gly Glu Thr Leu Ala Leu Leu Arg

35 40 45

Ala Gly Trp Asp Ser Glu Pro Glu Arg Phe Val Arg Glu Arg Ile Lys

50 55 60

Lys His Gly Ser Pro Leu Val Phe Lys Thr Ser Leu Phe Gly Asp Arg

65 70 75 80

Phe Ala Val Leu Cys Gly Pro Ala Gly Asn Lys Phe Leu Phe Cys Asn

85 90 95

Glu Asn Lys Leu Val Ala Ser Trp Trp Pro Val Pro Val Arg Lys Leu

100 105 110

Phe Gly Lys Ser Leu Leu Thr Ile Arg Gly Asp Glu Ala Lys Trp Met

115 120 125

Arg Lys Met Leu Leu Ser Tyr Leu Gly Pro Asp Ala Phe Ala Thr His

130 135 140

Tyr Ala Val Thr Met Asp Val Val Thr Arg Arg His Ile Asp Val His

145 150 155 160

Trp Arg Gly Lys Glu Glu Val Asn Val Phe Gln Thr Val Lys Leu Tyr

165 170 175

Ala Phe Glu Leu Ala Cys Arg Leu Phe Met Asn Leu Asp Asp Pro Asn

180 185 190

His Ile Ala Lys Leu Gly Ser Leu Phe Asn Ile Phe Leu Lys Gly Ile

195 200 205

Ile Glu Leu Pro Ile Asp Val Pro Gly Thr Arg Phe Tyr Ser Ser Lys

210 215 220

Lys Ala Ala Ala Ala Ile Arg Ile Glu Leu Lys Lys Leu Ile Lys Ala

225 230 235 240

Arg Lys Leu Glu Leu Lys Glu Gly Lys Ala Ser Ser Ser Gln Asp Leu

245 250 255

Leu Ser His Leu Leu Thr Ser Pro Asp Glu Asn Gly Met Phe Leu Thr

260 265 270

Glu Glu Glu Ile Val Asp Asn Ile Leu Leu Leu Leu Phe Ala Gly His

275 280 285

Asp Thr Ser Ala Leu Ser Ile Thr Leu Leu Met Lys Thr Leu Gly Glu

290 295 300

His Ser Asp Val Tyr Asp Lys Val Leu Lys Glu Gln Leu Glu Ile Ser

305 310 315 320

Lys Thr Lys Glu Ala Trp Glu Ser Leu Lys Trp Glu Asp Ile Gln Lys

325 330 335

Met Lys Tyr Ser Trp Ser Val Ile Cys Glu Val Met Arg Leu Asn Pro

340 345 350

Pro Val Ile Gly Thr Tyr Arg Glu Ala Leu Val Asp Ile Asp Tyr Ala

355 360 365

Gly Tyr Thr Ile Pro Lys Gly Trp Lys Leu His Trp Ser Ala Val Ser

370 375 380

Thr Gln Arg Asp Glu Ala Asn Phe Glu Asp Val Thr Arg Phe Asp Pro

385 390 395 400

Ser Arg Phe Glu Gly Ala Gly Pro Thr Pro Phe Thr Phe Val Pro Phe

405 410 415

Gly Gly Gly Pro Arg Met Cys Leu Gly Lys Glu Phe Ala Arg Leu Glu

420 425 430

Val Leu Ala Phe Leu His Asn Ile Val Thr Asn Phe Lys Trp Asp Leu

435 440 445

Leu Ile Pro Asp Glu Lys Ile Glu Tyr Asp Pro Met Ala Thr Pro Ala

450 455 460

Lys Gly Leu Pro Ile Arg Leu His Pro His Gln Val

465 470 475

<210> 93

<211> 1575

<212> DNA

<213> Arabidopsis thaliana

<220>

<221> CDS

<222> (1)..(1575)

<400> 93

atg gag tct tta gtg gtt cat aca gta aat gct atc tgg tgt att gta 48

Met Glu Ser Leu Val Val His Thr Val Asn Ala Ile Trp Cys Ile Val

1 5 10 15

atc gtc ggg att ttc tca gtt ggt tat cac gtt tac ggt aga gct gtg 96

Ile Val Gly Ile Phe Ser Val Gly Tyr His Val Tyr Gly Arg Ala Val

20 25 30

gtc gaa caa tgg aga atg aga aga tca ctg aag cta caa ggt gtt aaa 144

Val Glu Gln Trp Arg Met Arg Arg Ser Leu Lys Leu Gln Gly Val Lys

35 40 45

ggc cca cca cca tcc atc ttc aat ggt aac gtt tca gaa atg caa cgt 192

Gly Pro Pro Pro Ser Ile Phe Asn Gly Asn Val Ser Glu Met Gln Arg

50 55 60

atc caa tcc gaa gct aaa cac tgc tct ggc gat aac att atc tca cat 240

Ile Gln Ser Glu Ala Lys His Cys Ser Gly Asp Asn Ile Ile Ser His

65 70 75 80

gat tat tct tct tca tta ttc cca cac ttc gat cac tgg aga aaa cag 288

Asp Tyr Ser Ser Ser Leu Phe Pro His Phe Asp His Trp Arg Lys Gln

85 90 95

tac ggc aga atc tac aca tac tct act gga tta aag caa cac ttg tac 336

Tyr Gly Arg Ile Tyr Thr Tyr Ser Thr Gly Leu Lys Gln His Leu Tyr

100 105 110

atc aat cat cca gaa atg gtg aag gag cta tct cag act aac aca ttg 384

Ile Asn His Pro Glu Met Val Lys Glu Leu Ser Gln Thr Asn Thr Leu

115 120 125

aac ttg ggt aga atc acc cat ata acc aaa aga ttg aat cct atc tta 432

Asn Leu Gly Arg Ile Thr His Ile Thr Lys Arg Leu Asn Pro Ile Leu

130 135 140

ggt aac gga atc ata acc tct aat ggt cct cat tgg gcc cat cag cgt 480

Gly Asn Gly Ile Ile Thr Ser Asn Gly Pro His Trp Ala His Gln Arg

145 150 155 160

aga att atc gcc tac gag ttt act cat gat aag atc aag ggt atg gtt 528

Arg Ile Ile Ala Tyr Glu Phe Thr His Asp Lys Ile Lys Gly Met Val

165 170 175

ggt ttg atg gtt gag tct gct atg cct atg ttg aat aag tgg gag gag 576

Gly Leu Met Val Glu Ser Ala Met Pro Met Leu Asn Lys Trp Glu Glu

180 185 190

atg gta aag aga ggc gga gaa atg gga tgc gac ata aga gtt gat gag 624

Met Val Lys Arg Gly Gly Glu Met Gly Cys Asp Ile Arg Val Asp Glu

195 200 205

gac ttg aaa gat gtt tca gca gat gtg att gca aaa gcc tgt ttc gga 672

Asp Leu Lys Asp Val Ser Ala Asp Val Ile Ala Lys Ala Cys Phe Gly

210 215 220

tcc tca ttt tct aaa ggt aag gct att ttc tct atg ata aga gat ttg 720

Ser Ser Phe Ser Lys Gly Lys Ala Ile Phe Ser Met Ile Arg Asp Leu

225 230 235 240

ctt aca gct atc aca aag aga agt gtt cta ttc aga ttc aac gga ttc 768

Leu Thr Ala Ile Thr Lys Arg Ser Val Leu Phe Arg Phe Asn Gly Phe

245 250 255

act gat atg gtc ttt ggg agt aaa aag cat ggt gac gtt gat ata gac 816

Thr Asp Met Val Phe Gly Ser Lys Lys His Gly Asp Val Asp Ile Asp

260 265 270

gct tta gaa atg gaa ttg gaa tca tcc att tgg gaa act gtc aag gaa 864

Ala Leu Glu Met Glu Leu Glu Ser Ser Ile Trp Glu Thr Val Lys Glu

275 280 285

cgt gaa ata gaa tgt aaa gat act cac aaa aag gat ctg atg caa ttg 912

Arg Glu Ile Glu Cys Lys Asp Thr His Lys Lys Asp Leu Met Gln Leu

290 295 300

att ttg gaa ggg gca atg cgt tca tgt gac ggt aac ctt tgg gat aaa 960

Ile Leu Glu Gly Ala Met Arg Ser Cys Asp Gly Asn Leu Trp Asp Lys

305 310 315 320

tca gca tat aga aga ttt gtt gta gat aat tgt aaa tct atc tac ttc 1008

Ser Ala Tyr Arg Arg Phe Val Val Asp Asn Cys Lys Ser Ile Tyr Phe

325 330 335

gca ggg cat gat agt aca gct gtc tca gtg tca tgg tgt ttg atg tta 1056

Ala Gly His Asp Ser Thr Ala Val Ser Val Ser Trp Cys Leu Met Leu

340 345 350

ctg gcc cta aac cca tca tgg caa gtt aag atc cgt gat gaa att ctg 1104

Leu Ala Leu Asn Pro Ser Trp Gln Val Lys Ile Arg Asp Glu Ile Leu

355 360 365

tct tct tgc aaa aat ggt att cca gat gcc gaa agt atc cca aac ctt 1152

Ser Ser Cys Lys Asn Gly Ile Pro Asp Ala Glu Ser Ile Pro Asn Leu

370 375 380

aaa aca gtg act atg gtt att caa gag aca atg aga tta tac cct cca 1200

Lys Thr Val Thr Met Val Ile Gln Glu Thr Met Arg Leu Tyr Pro Pro

385 390 395 400

gca cca atc gtc ggg aga gaa gcc tct aaa gat atc aga ttg ggc gat 1248

Ala Pro Ile Val Gly Arg Glu Ala Ser Lys Asp Ile Arg Leu Gly Asp

405 410 415

cta gtt gtt cct aaa ggc gtc tgt ata tgg aca cta ata cca gct tta 1296

Leu Val Val Pro Lys Gly Val Cys Ile Trp Thr Leu Ile Pro Ala Leu

420 425 430

cac aga gat cct gag att tgg gga cca gat gca aac gat ttc aaa cca 1344

His Arg Asp Pro Glu Ile Trp Gly Pro Asp Ala Asn Asp Phe Lys Pro

435 440 445

gaa aga ttt tct gaa gga att tca aag gct tgt aag tat cct caa agt 1392

Glu Arg Phe Ser Glu Gly Ile Ser Lys Ala Cys Lys Tyr Pro Gln Ser

450 455 460

tac att cca ttt ggt ctg ggt cct aga aca tgc gtt ggt aaa aac ttt 1440

Tyr Ile Pro Phe Gly Leu Gly Pro Arg Thr Cys Val Gly Lys Asn Phe

465 470 475 480

ggc atg atg gaa gta aag gtt ctt gtt tcc ctg att gtc tcc aag ttc 1488

Gly Met Met Glu Val Lys Val Leu Val Ser Leu Ile Val Ser Lys Phe

485 490 495

tct ttc act cta tct cct acc tac caa cat agt cct agt cac aaa ctt 1536

Ser Phe Thr Leu Ser Pro Thr Tyr Gln His Ser Pro Ser His Lys Leu

500 505 510

tta gta gaa cca caa cat ggg gtg gta att aga gtg gtt 1575

Leu Val Glu Pro Gln His Gly Val Val Ile Arg Val Val

515 520 525

<210> 94

<211> 525

<212> PRT

<213> Arabidopsis thaliana

<400> 94

Met Glu Ser Leu Val Val His Thr Val Asn Ala Ile Trp Cys Ile Val

1 5 10 15

Ile Val Gly Ile Phe Ser Val Gly Tyr His Val Tyr Gly Arg Ala Val

20 25 30

Val Glu Gln Trp Arg Met Arg Arg Ser Leu Lys Leu Gln Gly Val Lys

35 40 45

Gly Pro Pro Pro Ser Ile Phe Asn Gly Asn Val Ser Glu Met Gln Arg

50 55 60

Ile Gln Ser Glu Ala Lys His Cys Ser Gly Asp Asn Ile Ile Ser His

65 70 75 80

Asp Tyr Ser Ser Ser Leu Phe Pro His Phe Asp His Trp Arg Lys Gln

85 90 95

Tyr Gly Arg Ile Tyr Thr Tyr Ser Thr Gly Leu Lys Gln His Leu Tyr

100 105 110

Ile Asn His Pro Glu Met Val Lys Glu Leu Ser Gln Thr Asn Thr Leu

115 120 125

Asn Leu Gly Arg Ile Thr His Ile Thr Lys Arg Leu Asn Pro Ile Leu

130 135 140

Gly Asn Gly Ile Ile Thr Ser Asn Gly Pro His Trp Ala His Gln Arg

145 150 155 160

Arg Ile Ile Ala Tyr Glu Phe Thr His Asp Lys Ile Lys Gly Met Val

165 170 175

Gly Leu Met Val Glu Ser Ala Met Pro Met Leu Asn Lys Trp Glu Glu

180 185 190

Met Val Lys Arg Gly Gly Glu Met Gly Cys Asp Ile Arg Val Asp Glu

195 200 205

Asp Leu Lys Asp Val Ser Ala Asp Val Ile Ala Lys Ala Cys Phe Gly

210 215 220

Ser Ser Phe Ser Lys Gly Lys Ala Ile Phe Ser Met Ile Arg Asp Leu

225 230 235 240

Leu Thr Ala Ile Thr Lys Arg Ser Val Leu Phe Arg Phe Asn Gly Phe

245 250 255

Thr Asp Met Val Phe Gly Ser Lys Lys His Gly Asp Val Asp Ile Asp

260 265 270

Ala Leu Glu Met Glu Leu Glu Ser Ser Ile Trp Glu Thr Val Lys Glu

275 280 285

Arg Glu Ile Glu Cys Lys Asp Thr His Lys Lys Asp Leu Met Gln Leu

290 295 300

Ile Leu Glu Gly Ala Met Arg Ser Cys Asp Gly Asn Leu Trp Asp Lys

305 310 315 320

Ser Ala Tyr Arg Arg Phe Val Val Asp Asn Cys Lys Ser Ile Tyr Phe

325 330 335

Ala Gly His Asp Ser Thr Ala Val Ser Val Ser Trp Cys Leu Met Leu

340 345 350

Leu Ala Leu Asn Pro Ser Trp Gln Val Lys Ile Arg Asp Glu Ile Leu

355 360 365

Ser Ser Cys Lys Asn Gly Ile Pro Asp Ala Glu Ser Ile Pro Asn Leu

370 375 380

Lys Thr Val Thr Met Val Ile Gln Glu Thr Met Arg Leu Tyr Pro Pro

385 390 395 400

Ala Pro Ile Val Gly Arg Glu Ala Ser Lys Asp Ile Arg Leu Gly Asp

405 410 415

Leu Val Val Pro Lys Gly Val Cys Ile Trp Thr Leu Ile Pro Ala Leu

420 425 430

His Arg Asp Pro Glu Ile Trp Gly Pro Asp Ala Asn Asp Phe Lys Pro

435 440 445

Glu Arg Phe Ser Glu Gly Ile Ser Lys Ala Cys Lys Tyr Pro Gln Ser

450 455 460

Tyr Ile Pro Phe Gly Leu Gly Pro Arg Thr Cys Val Gly Lys Asn Phe

465 470 475 480

Gly Met Met Glu Val Lys Val Leu Val Ser Leu Ile Val Ser Lys Phe

485 490 495

Ser Phe Thr Leu Ser Pro Thr Tyr Gln His Ser Pro Ser His Lys Leu

500 505 510

Leu Val Glu Pro Gln His Gly Val Val Ile Arg Val Val

515 520 525

<210> 95

<211> 1587

<212> DNA

<213> Vitis vinifera

<220>

<221> CDS

<222> (1)..(1587)

<400> 95

atg tac ttc cta cta caa tac ctc aac atc aca acc gtt ggt gtc ttt 48

Met Tyr Phe Leu Leu Gln Tyr Leu Asn Ile Thr Thr Val Gly Val Phe

1 5 10 15

gcc aca ttg ttt ctc tct tat tgt tta ctt ctc tgg aga agt aga gcg 96

Ala Thr Leu Phe Leu Ser Tyr Cys Leu Leu Leu Trp Arg Ser Arg Ala

20 25 30

ggt aac aaa aag att gcc cca gaa gct gcc gct gca tgg cct att atc 144

Gly Asn Lys Lys Ile Ala Pro Glu Ala Ala Ala Ala Trp Pro Ile Ile

35 40 45

ggc cac ctc cac tta ctt gca ggt gga tcc cat caa cta cca cat att 192

Gly His Leu His Leu Leu Ala Gly Gly Ser His Gln Leu Pro His Ile

50 55 60

aca ttg ggt aac atg gca gat aag tac ggt cct gta ttc aca atc aga 240

Thr Leu Gly Asn Met Ala Asp Lys Tyr Gly Pro Val Phe Thr Ile Arg

65 70 75 80

ata ggc ttg cat aga gct gta gtt gtc tca tct tgg gaa atg gca aag 288

Ile Gly Leu His Arg Ala Val Val Val Ser Ser Trp Glu Met Ala Lys

85 90 95

gaa tgt tca aca gct aat gat caa gtg tct tct tca aga cct gaa cta 336

Glu Cys Ser Thr Ala Asn Asp Gln Val Ser Ser Ser Arg Pro Glu Leu

100 105 110

tta gct tct aag ttg ttg ggt tat aac tac gcc atg ttt ggt ttt tca 384

Leu Ala Ser Lys Leu Leu Gly Tyr Asn Tyr Ala Met Phe Gly Phe Ser

115 120 125

cca tac ggt tca tac tgg aga gaa atg aga aag atc atc tct ctc gaa 432

Pro Tyr Gly Ser Tyr Trp Arg Glu Met Arg Lys Ile Ile Ser Leu Glu

130 135 140

tta cta tct aat tcc aga ttg gaa cta ttg aaa gat gtt aga gcc tca 480

Leu Leu Ser Asn Ser Arg Leu Glu Leu Leu Lys Asp Val Arg Ala Ser

145 150 155 160

gaa gtt gtc aca tct att aag gaa cta tac aaa ttg tgg gcg gaa aag 528

Glu Val Val Thr Ser Ile Lys Glu Leu Tyr Lys Leu Trp Ala Glu Lys

165 170 175

aag aat gag tca gga ttg gtt tct gtc gag atg aaa caa tgg ttc gga 576

Lys Asn Glu Ser Gly Leu Val Ser Val Glu Met Lys Gln Trp Phe Gly

180 185 190

gat ttg act tta aac gtg atc ttg aga atg gtg gct ggt aaa aga tac 624

Asp Leu Thr Leu Asn Val Ile Leu Arg Met Val Ala Gly Lys Arg Tyr

195 200 205

ttc tcc gcg agt gac gct tca gaa aac aaa cag gcc cag cgt tgt aga 672

Phe Ser Ala Ser Asp Ala Ser Glu Asn Lys Gln Ala Gln Arg Cys Arg

210 215 220

aga gtc ttc aga gaa ttc ttc cat ctc tcc ggc ttg ttt gtg gtt gct 720

Arg Val Phe Arg Glu Phe Phe His Leu Ser Gly Leu Phe Val Val Ala

225 230 235 240

gat gct ata cct ttt ctt gga tgg ctc gat tgg gga aga cac gag aag 768

Asp Ala Ile Pro Phe Leu Gly Trp Leu Asp Trp Gly Arg His Glu Lys

245 250 255

acc ttg aaa aag acc gcc ata gaa atg gat tcc atc gcc cag gag tgg 816

Thr Leu Lys Lys Thr Ala Ile Glu Met Asp Ser Ile Ala Gln Glu Trp

260 265 270

ctt gag gaa cat aga cgt aga aaa gat tct gga gat gat aat tct acc 864

Leu Glu Glu His Arg Arg Arg Lys Asp Ser Gly Asp Asp Asn Ser Thr

275 280 285

caa gat ttc atg gac gtt atg caa tct gtg cta gat ggc aaa aat cta 912

Gln Asp Phe Met Asp Val Met Gln Ser Val Leu Asp Gly Lys Asn Leu

290 295 300

ggc gga tac gat gct gat acg att aac aag gct aca tgc tta act ctt 960

Gly Gly Tyr Asp Ala Asp Thr Ile Asn Lys Ala Thr Cys Leu Thr Leu

305 310 315 320

ata tca ggt ggc agt gat act act gta gtt tct ttg aca tgg gct ctt 1008

Ile Ser Gly Gly Ser Asp Thr Thr Val Val Ser Leu Thr Trp Ala Leu

325 330 335

agt ctt gtg tta aac aat aga gat act ttg aaa aag gca cag gaa gag 1056

Ser Leu Val Leu Asn Asn Arg Asp Thr Leu Lys Lys Ala Gln Glu Glu

340 345 350

tta gac atc caa gtc ggt aag gaa aga ttg gtt aac gag caa gac atc 1104

Leu Asp Ile Gln Val Gly Lys Glu Arg Leu Val Asn Glu Gln Asp Ile

355 360 365

agt aag tta gtt tac ttg caa gca ata gta aaa gag aca ctc aga ctt 1152

Ser Lys Leu Val Tyr Leu Gln Ala Ile Val Lys Glu Thr Leu Arg Leu

370 375 380

tat cca cca ggt cct ttg ggt ggt ttg aga caa ttc act gaa gat tgt 1200

Tyr Pro Pro Gly Pro Leu Gly Gly Leu Arg Gln Phe Thr Glu Asp Cys

385 390 395 400

aca cta ggt ggc tat cac gtt tca aaa gga act aga tta atc atg aac 1248

Thr Leu Gly Gly Tyr His Val Ser Lys Gly Thr Arg Leu Ile Met Asn

405 410 415

tta tcc aag att caa aaa gat cca cgt att tgg tct gat cct act gaa 1296

Leu Ser Lys Ile Gln Lys Asp Pro Arg Ile Trp Ser Asp Pro Thr Glu

420 425 430

ttc caa cca gag aga ttc ctt acg act cat aaa gat gtc gat cca cgt 1344

Phe Gln Pro Glu Arg Phe Leu Thr Thr His Lys Asp Val Asp Pro Arg

435 440 445

ggt aaa cac ttt gaa ttc att cca ttc ggt gca gga aga cgt gca tgt 1392

Gly Lys His Phe Glu Phe Ile Pro Phe Gly Ala Gly Arg Arg Ala Cys

450 455 460

cct ggt atc aca ttc gga tta caa gta cta cat cta aca ttg gca tct 1440

Pro Gly Ile Thr Phe Gly Leu Gln Val Leu His Leu Thr Leu Ala Ser

465 470 475 480

ttc ttg cat gcg ttt gaa ttt tca aca cca tca aat gag cag gtt aac 1488

Phe Leu His Ala Phe Glu Phe Ser Thr Pro Ser Asn Glu Gln Val Asn

485 490 495

atg aga gaa tca tta ggt ctt acg aat atg aaa tct acc cca tta gaa 1536

Met Arg Glu Ser Leu Gly Leu Thr Asn Met Lys Ser Thr Pro Leu Glu

500 505 510

gtt ttg att tct cca aga cta tcc ctt aat tgc ttc aac ctt atg aaa 1584

Val Leu Ile Ser Pro Arg Leu Ser Leu Asn Cys Phe Asn Leu Met Lys

515 520 525

att 1587

Ile

<210> 96

<211> 529

<212> PRT

<213> Vitis vinifera

<400> 96

Met Tyr Phe Leu Leu Gln Tyr Leu Asn Ile Thr Thr Val Gly Val Phe

1 5 10 15

Ala Thr Leu Phe Leu Ser Tyr Cys Leu Leu Leu Trp Arg Ser Arg Ala

20 25 30

Gly Asn Lys Lys Ile Ala Pro Glu Ala Ala Ala Ala Trp Pro Ile Ile

35 40 45

Gly His Leu His Leu Leu Ala Gly Gly Ser His Gln Leu Pro His Ile

50 55 60

Thr Leu Gly Asn Met Ala Asp Lys Tyr Gly Pro Val Phe Thr Ile Arg

65 70 75 80

Ile Gly Leu His Arg Ala Val Val Val Ser Ser Trp Glu Met Ala Lys

85 90 95

Glu Cys Ser Thr Ala Asn Asp Gln Val Ser Ser Ser Arg Pro Glu Leu

100 105 110

Leu Ala Ser Lys Leu Leu Gly Tyr Asn Tyr Ala Met Phe Gly Phe Ser

115 120 125

Pro Tyr Gly Ser Tyr Trp Arg Glu Met Arg Lys Ile Ile Ser Leu Glu

130 135 140

Leu Leu Ser Asn Ser Arg Leu Glu Leu Leu Lys Asp Val Arg Ala Ser

145 150 155 160

Glu Val Val Thr Ser Ile Lys Glu Leu Tyr Lys Leu Trp Ala Glu Lys

165 170 175

Lys Asn Glu Ser Gly Leu Val Ser Val Glu Met Lys Gln Trp Phe Gly

180 185 190

Asp Leu Thr Leu Asn Val Ile Leu Arg Met Val Ala Gly Lys Arg Tyr

195 200 205

Phe Ser Ala Ser Asp Ala Ser Glu Asn Lys Gln Ala Gln Arg Cys Arg

210 215 220

Arg Val Phe Arg Glu Phe Phe His Leu Ser Gly Leu Phe Val Val Ala

225 230 235 240

Asp Ala Ile Pro Phe Leu Gly Trp Leu Asp Trp Gly Arg His Glu Lys

245 250 255

Thr Leu Lys Lys Thr Ala Ile Glu Met Asp Ser Ile Ala Gln Glu Trp

260 265 270

Leu Glu Glu His Arg Arg Arg Lys Asp Ser Gly Asp Asp Asn Ser Thr

275 280 285

Gln Asp Phe Met Asp Val Met Gln Ser Val Leu Asp Gly Lys Asn Leu

290 295 300

Gly Gly Tyr Asp Ala Asp Thr Ile Asn Lys Ala Thr Cys Leu Thr Leu

305 310 315 320

Ile Ser Gly Gly Ser Asp Thr Thr Val Val Ser Leu Thr Trp Ala Leu

325 330 335

Ser Leu Val Leu Asn Asn Arg Asp Thr Leu Lys Lys Ala Gln Glu Glu

340 345 350

Leu Asp Ile Gln Val Gly Lys Glu Arg Leu Val Asn Glu Gln Asp Ile

355 360 365

Ser Lys Leu Val Tyr Leu Gln Ala Ile Val Lys Glu Thr Leu Arg Leu

370 375 380

Tyr Pro Pro Gly Pro Leu Gly Gly Leu Arg Gln Phe Thr Glu Asp Cys

385 390 395 400

Thr Leu Gly Gly Tyr His Val Ser Lys Gly Thr Arg Leu Ile Met Asn

405 410 415

Leu Ser Lys Ile Gln Lys Asp Pro Arg Ile Trp Ser Asp Pro Thr Glu

420 425 430

Phe Gln Pro Glu Arg Phe Leu Thr Thr His Lys Asp Val Asp Pro Arg

435 440 445

Gly Lys His Phe Glu Phe Ile Pro Phe Gly Ala Gly Arg Arg Ala Cys

450 455 460

Pro Gly Ile Thr Phe Gly Leu Gln Val Leu His Leu Thr Leu Ala Ser

465 470 475 480

Phe Leu His Ala Phe Glu Phe Ser Thr Pro Ser Asn Glu Gln Val Asn

485 490 495

Met Arg Glu Ser Leu Gly Leu Thr Asn Met Lys Ser Thr Pro Leu Glu

500 505 510

Val Leu Ile Ser Pro Arg Leu Ser Leu Asn Cys Phe Asn Leu Met Lys

515 520 525

Ile

<210> 97

<211> 1437

<212> DNA

<213> Medicago truncatula

<220>

<221> CDS

<222> (1)..(1437)

<400> 97

atg gaa cct aac ttt tac ttg tca tta cta ttg ttg ttc gtg acc ttc 48

Met Glu Pro Asn Phe Tyr Leu Ser Leu Leu Leu Leu Phe Val Thr Phe

1 5 10 15

att tct tta agt ctg ttt ttc atc ttt tac aaa caa aag tcc cca ttg 96

Ile Ser Leu Ser Leu Phe Phe Ile Phe Tyr Lys Gln Lys Ser Pro Leu

20 25 30

aat ttg cca cca ggg aaa atg ggt tac cct atc ata ggt gaa agt tta 144

Asn Leu Pro Pro Gly Lys Met Gly Tyr Pro Ile Ile Gly Glu Ser Leu

35 40 45

gaa ttc cta tcc aca ggc tgg aag gga cat cct gaa aag ttc ata ttt 192

Glu Phe Leu Ser Thr Gly Trp Lys Gly His Pro Glu Lys Phe Ile Phe

50 55 60

gat aga atg cgt aag tac agt agt gag tta ttc aag act tct att gta 240

Asp Arg Met Arg Lys Tyr Ser Ser Glu Leu Phe Lys Thr Ser Ile Val

65 70 75 80

ggc gaa tcc aca gtt gtt tgc tgt ggg gca gct agt aac aaa ttc cta 288

Gly Glu Ser Thr Val Val Cys Cys Gly Ala Ala Ser Asn Lys Phe Leu

85 90 95

ttc tct aac gaa aac aaa ctg gta act gcc tgg tgg cca gat tct gtt 336

Phe Ser Asn Glu Asn Lys Leu Val Thr Ala Trp Trp Pro Asp Ser Val

100 105 110

aac aaa atc ttc cca aca act tca ctg gat tct aat ttg aag gag gaa 384

Asn Lys Ile Phe Pro Thr Thr Ser Leu Asp Ser Asn Leu Lys Glu Glu

115 120 125

tct ata aag atg aga aag ttg ctg cca cag ttc ttc aaa cca gaa gca 432

Ser Ile Lys Met Arg Lys Leu Leu Pro Gln Phe Phe Lys Pro Glu Ala

130 135 140

ctt caa aga tac gtc ggc gtt atg gat gta atc gca caa aga cat ttt 480

Leu Gln Arg Tyr Val Gly Val Met Asp Val Ile Ala Gln Arg His Phe

145 150 155 160

gtc act cac tgg gac aac aaa aat gag atc aca gtt tat cca ctt gct 528

Val Thr His Trp Asp Asn Lys Asn Glu Ile Thr Val Tyr Pro Leu Ala

165 170 175

aaa aga tac act ttc ttg ctt gcg tgt aga ctg ttc atg tct gtt gag 576

Lys Arg Tyr Thr Phe Leu Leu Ala Cys Arg Leu Phe Met Ser Val Glu

180 185 190

gat gaa aat cat gtg gcg aaa ttc tca gac cca ttc caa cta atc gct 624

Asp Glu Asn His Val Ala Lys Phe Ser Asp Pro Phe Gln Leu Ile Ala

195 200 205

gca ggc atc att tca ctt cct atc gat ctt cct ggt act cca ttc aac 672

Ala Gly Ile Ile Ser Leu Pro Ile Asp Leu Pro Gly Thr Pro Phe Asn

210 215 220

aag gcc ata aag gct tca aat ttc att aga aaa gag ctg ata aag att 720

Lys Ala Ile Lys Ala Ser Asn Phe Ile Arg Lys Glu Leu Ile Lys Ile

225 230 235 240

atc aaa caa aga cgt gtt gat ctg gca gag ggt aca gca tct cca acc 768

Ile Lys Gln Arg Arg Val Asp Leu Ala Glu Gly Thr Ala Ser Pro Thr

245 250 255

cag gat atc ttg tca cat atg cta tta aca tct gat gaa aac ggt aaa 816

Gln Asp Ile Leu Ser His Met Leu Leu Thr Ser Asp Glu Asn Gly Lys

260 265 270

tct atg aac gag ttg aac att gcc gac aag att ctt gga cta ttg ata 864

Ser Met Asn Glu Leu Asn Ile Ala Asp Lys Ile Leu Gly Leu Leu Ile

275 280 285

gga ggc cac gat aca gct tca gta gct tgc aca ttt cta gtg aag tac 912

Gly Gly His Asp Thr Ala Ser Val Ala Cys Thr Phe Leu Val Lys Tyr

290 295 300

tta gga gaa tta cca cat atc tac gat aaa gtc tac caa gag caa atg 960

Leu Gly Glu Leu Pro His Ile Tyr Asp Lys Val Tyr Gln Glu Gln Met

305 310 315 320

gaa att gcc aag tcc aaa cct gct ggg gaa ttg ttg aat tgg gat gac 1008

Glu Ile Ala Lys Ser Lys Pro Ala Gly Glu Leu Leu Asn Trp Asp Asp

325 330 335

ttg aaa aag atg aag tat tca tgg aat gtg gca tgt gag gta atg aga 1056

Leu Lys Lys Met Lys Tyr Ser Trp Asn Val Ala Cys Glu Val Met Arg

340 345 350

ttg tca cca cct tta caa ggt ggt ttt aga gag gct ata act gac ttt 1104

Leu Ser Pro Pro Leu Gln Gly Gly Phe Arg Glu Ala Ile Thr Asp Phe

355 360 365

atg ttt aac ggt ttc tct att cca aaa ggg tgg aag tta tac tgg tcc 1152

Met Phe Asn Gly Phe Ser Ile Pro Lys Gly Trp Lys Leu Tyr Trp Ser

370 375 380

gcc aac tct aca cac aaa aat gca gaa tgt ttc cca atg cct gag aaa 1200

Ala Asn Ser Thr His Lys Asn Ala Glu Cys Phe Pro Met Pro Glu Lys

385 390 395 400

ttc gat cct acc aga ttt gaa ggt aat ggt cca gcg cct tat aca ttt 1248

Phe Asp Pro Thr Arg Phe Glu Gly Asn Gly Pro Ala Pro Tyr Thr Phe

405 410 415

gta cca ttc ggt gga ggc cct aga atg tgt cct gga aag gaa tac gct 1296

Val Pro Phe Gly Gly Gly Pro Arg Met Cys Pro Gly Lys Glu Tyr Ala

420 425 430

aga tta gaa atc ttg gtt ttc atg cat aat ctg gtc aaa cgt ttt aag 1344

Arg Leu Glu Ile Leu Val Phe Met His Asn Leu Val Lys Arg Phe Lys

435 440 445

tgg gaa aag gtt att cca gac gaa aag att att gtc gat cca ttc cca 1392

Trp Glu Lys Val Ile Pro Asp Glu Lys Ile Ile Val Asp Pro Phe Pro

450 455 460

atc cca gct aaa gat ctt cca atc cgt ttg tat cct cac aaa gct 1437

Ile Pro Ala Lys Asp Leu Pro Ile Arg Leu Tyr Pro His Lys Ala

465 470 475

<210> 98

<211> 479

<212> PRT

<213> Medicago truncatula

<400> 98

Met Glu Pro Asn Phe Tyr Leu Ser Leu Leu Leu Leu Phe Val Thr Phe

1 5 10 15

Ile Ser Leu Ser Leu Phe Phe Ile Phe Tyr Lys Gln Lys Ser Pro Leu

20 25 30

Asn Leu Pro Pro Gly Lys Met Gly Tyr Pro Ile Ile Gly Glu Ser Leu

35 40 45

Glu Phe Leu Ser Thr Gly Trp Lys Gly His Pro Glu Lys Phe Ile Phe

50 55 60

Asp Arg Met Arg Lys Tyr Ser Ser Glu Leu Phe Lys Thr Ser Ile Val

65 70 75 80

Gly Glu Ser Thr Val Val Cys Cys Gly Ala Ala Ser Asn Lys Phe Leu

85 90 95

Phe Ser Asn Glu Asn Lys Leu Val Thr Ala Trp Trp Pro Asp Ser Val

100 105 110

Asn Lys Ile Phe Pro Thr Thr Ser Leu Asp Ser Asn Leu Lys Glu Glu

115 120 125

Ser Ile Lys Met Arg Lys Leu Leu Pro Gln Phe Phe Lys Pro Glu Ala

130 135 140

Leu Gln Arg Tyr Val Gly Val Met Asp Val Ile Ala Gln Arg His Phe

145 150 155 160

Val Thr His Trp Asp Asn Lys Asn Glu Ile Thr Val Tyr Pro Leu Ala

165 170 175

Lys Arg Tyr Thr Phe Leu Leu Ala Cys Arg Leu Phe Met Ser Val Glu

180 185 190

Asp Glu Asn His Val Ala Lys Phe Ser Asp Pro Phe Gln Leu Ile Ala

195 200 205

Ala Gly Ile Ile Ser Leu Pro Ile Asp Leu Pro Gly Thr Pro Phe Asn

210 215 220

Lys Ala Ile Lys Ala Ser Asn Phe Ile Arg Lys Glu Leu Ile Lys Ile

225 230 235 240

Ile Lys Gln Arg Arg Val Asp Leu Ala Glu Gly Thr Ala Ser Pro Thr

245 250 255

Gln Asp Ile Leu Ser His Met Leu Leu Thr Ser Asp Glu Asn Gly Lys

260 265 270

Ser Met Asn Glu Leu Asn Ile Ala Asp Lys Ile Leu Gly Leu Leu Ile

275 280 285

Gly Gly His Asp Thr Ala Ser Val Ala Cys Thr Phe Leu Val Lys Tyr

290 295 300

Leu Gly Glu Leu Pro His Ile Tyr Asp Lys Val Tyr Gln Glu Gln Met

305 310 315 320

Glu Ile Ala Lys Ser Lys Pro Ala Gly Glu Leu Leu Asn Trp Asp Asp

325 330 335

Leu Lys Lys Met Lys Tyr Ser Trp Asn Val Ala Cys Glu Val Met Arg

340 345 350

Leu Ser Pro Pro Leu Gln Gly Gly Phe Arg Glu Ala Ile Thr Asp Phe

355 360 365

Met Phe Asn Gly Phe Ser Ile Pro Lys Gly Trp Lys Leu Tyr Trp Ser

370 375 380

Ala Asn Ser Thr His Lys Asn Ala Glu Cys Phe Pro Met Pro Glu Lys

385 390 395 400

Phe Asp Pro Thr Arg Phe Glu Gly Asn Gly Pro Ala Pro Tyr Thr Phe

405 410 415

Val Pro Phe Gly Gly Gly Pro Arg Met Cys Pro Gly Lys Glu Tyr Ala

420 425 430

Arg Leu Glu Ile Leu Val Phe Met His Asn Leu Val Lys Arg Phe Lys

435 440 445

Trp Glu Lys Val Ile Pro Asp Glu Lys Ile Ile Val Asp Pro Phe Pro

450 455 460

Ile Pro Ala Lys Asp Leu Pro Ile Arg Leu Tyr Pro His Lys Ala

465 470 475

<210> 99

<211> 1419

<212> DNA

<213> Stevia rebaudiana

<220>

<221> CDS

<222> (1)..(1419)

<400> 99

atg gct aca tct gat tct att gtt gat gac agg aag cag ttg cat gtg 48

Met Ala Thr Ser Asp Ser Ile Val Asp Asp Arg Lys Gln Leu His Val

1 5 10 15

gct act ttc cct tgg ctt gct ttc ggt cat ata ctg cct tac cta caa 96

Ala Thr Phe Pro Trp Leu Ala Phe Gly His Ile Leu Pro Tyr Leu Gln

20 25 30

cta tca aaa ctg ata gct gaa aaa gga cat aaa gtg tca ttc ctt tca 144

Leu Ser Lys Leu Ile Ala Glu Lys Gly His Lys Val Ser Phe Leu Ser

35 40 45

aca act aga aac att caa aga tta tct tcc cac ata tca cca ttg att 192

Thr Thr Arg Asn Ile Gln Arg Leu Ser Ser His Ile Ser Pro Leu Ile

50 55 60

aac gtc gtt caa ttg aca ctt cca aga gta cag gaa tta cca gaa gat 240

Asn Val Val Gln Leu Thr Leu Pro Arg Val Gln Glu Leu Pro Glu Asp

65 70 75 80

gct gaa gct aca aca gat gtg cat cct gaa gat atc cct tac ttg aaa 288

Ala Glu Ala Thr Thr Asp Val His Pro Glu Asp Ile Pro Tyr Leu Lys

85 90 95

aag gca tcc gat gga tta cag cct gag gtc act aga ttc ctt gag caa 336

Lys Ala Ser Asp Gly Leu Gln Pro Glu Val Thr Arg Phe Leu Glu Gln

100 105 110

cac agt cca gat tgg atc ata tac gac tac act cac tat tgg ttg cct 384

His Ser Pro Asp Trp Ile Ile Tyr Asp Tyr Thr His Tyr Trp Leu Pro

115 120 125

tca att gca gca tca cta ggc att tct agg gca cat ttc agt gta acc 432

Ser Ile Ala Ala Ser Leu Gly Ile Ser Arg Ala His Phe Ser Val Thr

130 135 140

aca cct tgg gcc att gct tac atg ggt cca tcc gct gat gct atg att 480

Thr Pro Trp Ala Ile Ala Tyr Met Gly Pro Ser Ala Asp Ala Met Ile

145 150 155 160

aac ggc agt gat ggt aga act acc gtt gaa gat ttg aca acc cca cca 528

Asn Gly Ser Asp Gly Arg Thr Thr Val Glu Asp Leu Thr Thr Pro Pro

165 170 175

aag tgg ttt cca ttt cca act aaa gtc tgt tgg aga aaa cac gac tta 576

Lys Trp Phe Pro Phe Pro Thr Lys Val Cys Trp Arg Lys His Asp Leu

180 185 190

gca aga ctg gtt cca tac aag gca cca gga atc tca gac ggc tat aga 624

Ala Arg Leu Val Pro Tyr Lys Ala Pro Gly Ile Ser Asp Gly Tyr Arg

195 200 205

atg ggt tta gtc ctt aaa ggg tct gac tgc cta ttg tct aag tgt tac 672

Met Gly Leu Val Leu Lys Gly Ser Asp Cys Leu Leu Ser Lys Cys Tyr

210 215 220

cat gag ttt ggg aca caa tgg cta cca ctt ttg gaa aca tta cac caa 720

His Glu Phe Gly Thr Gln Trp Leu Pro Leu Leu Glu Thr Leu His Gln

225 230 235 240

gtt cct gtc gta cca gtt ggt cta tta cct cca gaa atc cct ggt gat 768

Val Pro Val Val Pro Val Gly Leu Leu Pro Pro Glu Ile Pro Gly Asp

245 250 255

gag aag gac gag act tgg gtt tca atc aaa aag tgg tta gac ggg aag 816

Glu Lys Asp Glu Thr Trp Val Ser Ile Lys Lys Trp Leu Asp Gly Lys

260 265 270

caa aaa ggc tca gtg gta tat gtg gca ctg ggt tcc gaa gtt tta gta 864

Gln Lys Gly Ser Val Val Tyr Val Ala Leu Gly Ser Glu Val Leu Val

275 280 285

tct caa aca gaa gtt gtg gaa ctt gcc tta ggt ttg gaa cta tct gga 912

Ser Gln Thr Glu Val Val Glu Leu Ala Leu Gly Leu Glu Leu Ser Gly

290 295 300

ttg cca ttt gtc tgg gcc tac aga aaa cca aaa ggc cct gca aag tcc 960

Leu Pro Phe Val Trp Ala Tyr Arg Lys Pro Lys Gly Pro Ala Lys Ser

305 310 315 320

gat tca gtt gaa ttg cca gac ggc ttt gtc gag aga act aga gat aga 1008

Asp Ser Val Glu Leu Pro Asp Gly Phe Val Glu Arg Thr Arg Asp Arg

325 330 335

ggg ttg gta tgg act tca tgg gct cca caa ttg aga atc ctg agt cac 1056

Gly Leu Val Trp Thr Ser Trp Ala Pro Gln Leu Arg Ile Leu Ser His

340 345 350

gaa tct gtg tgc ggt ttc cta aca cat tgt ggt tct ggt tct ata gtt 1104

Glu Ser Val Cys Gly Phe Leu Thr His Cys Gly Ser Gly Ser Ile Val

355 360 365

gaa gga ctg atg ttt ggt cat cca ctt atc atg ttg cca atc ttt ggt 1152

Glu Gly Leu Met Phe Gly His Pro Leu Ile Met Leu Pro Ile Phe Gly

370 375 380

gac cag cct ttg aat gca cgt ctg tta gaa gat aaa caa gtt gga att 1200

Asp Gln Pro Leu Asn Ala Arg Leu Leu Glu Asp Lys Gln Val Gly Ile

385 390 395 400

gaa atc cca cgt aat gag gaa gat gga tgt tta acc aag gag tct gtg 1248

Glu Ile Pro Arg Asn Glu Glu Asp Gly Cys Leu Thr Lys Glu Ser Val

405 410 415

gcc aga tca tta cgt tcc gtt gtc gtt gaa aag gaa ggc gaa atc tac 1296

Ala Arg Ser Leu Arg Ser Val Val Val Glu Lys Glu Gly Glu Ile Tyr

420 425 430

aag gcc aat gcc cgt gaa ctt tca aag atc tac aat gac aca aaa gta 1344

Lys Ala Asn Ala Arg Glu Leu Ser Lys Ile Tyr Asn Asp Thr Lys Val

435 440 445

gag aag gaa tat gtt tct caa ttt gta gat tac cta gag aaa aac gct 1392

Glu Lys Glu Tyr Val Ser Gln Phe Val Asp Tyr Leu Glu Lys Asn Ala

450 455 460

aga gcc gta gct att gat cat gaa tcc 1419

Arg Ala Val Ala Ile Asp His Glu Ser

465 470

<210> 100

<211> 473

<212> PRT

<213> Stevia rebaudiana

<400> 100

Met Ala Thr Ser Asp Ser Ile Val Asp Asp Arg Lys Gln Leu His Val

1 5 10 15

Ala Thr Phe Pro Trp Leu Ala Phe Gly His Ile Leu Pro Tyr Leu Gln

20 25 30

Leu Ser Lys Leu Ile Ala Glu Lys Gly His Lys Val Ser Phe Leu Ser

35 40 45

Thr Thr Arg Asn Ile Gln Arg Leu Ser Ser His Ile Ser Pro Leu Ile

50 55 60

Asn Val Val Gln Leu Thr Leu Pro Arg Val Gln Glu Leu Pro Glu Asp

65 70 75 80

Ala Glu Ala Thr Thr Asp Val His Pro Glu Asp Ile Pro Tyr Leu Lys

85 90 95

Lys Ala Ser Asp Gly Leu Gln Pro Glu Val Thr Arg Phe Leu Glu Gln

100 105 110

His Ser Pro Asp Trp Ile Ile Tyr Asp Tyr Thr His Tyr Trp Leu Pro

115 120 125

Ser Ile Ala Ala Ser Leu Gly Ile Ser Arg Ala His Phe Ser Val Thr

130 135 140

Thr Pro Trp Ala Ile Ala Tyr Met Gly Pro Ser Ala Asp Ala Met Ile

145 150 155 160

Asn Gly Ser Asp Gly Arg Thr Thr Val Glu Asp Leu Thr Thr Pro Pro

165 170 175

Lys Trp Phe Pro Phe Pro Thr Lys Val Cys Trp Arg Lys His Asp Leu

180 185 190

Ala Arg Leu Val Pro Tyr Lys Ala Pro Gly Ile Ser Asp Gly Tyr Arg

195 200 205

Met Gly Leu Val Leu Lys Gly Ser Asp Cys Leu Leu Ser Lys Cys Tyr

210 215 220

His Glu Phe Gly Thr Gln Trp Leu Pro Leu Leu Glu Thr Leu His Gln

225 230 235 240

Val Pro Val Val Pro Val Gly Leu Leu Pro Pro Glu Ile Pro Gly Asp

245 250 255

Glu Lys Asp Glu Thr Trp Val Ser Ile Lys Lys Trp Leu Asp Gly Lys

260 265 270

Gln Lys Gly Ser Val Val Tyr Val Ala Leu Gly Ser Glu Val Leu Val

275 280 285

Ser Gln Thr Glu Val Val Glu Leu Ala Leu Gly Leu Glu Leu Ser Gly

290 295 300

Leu Pro Phe Val Trp Ala Tyr Arg Lys Pro Lys Gly Pro Ala Lys Ser

305 310 315 320

Asp Ser Val Glu Leu Pro Asp Gly Phe Val Glu Arg Thr Arg Asp Arg

325 330 335

Gly Leu Val Trp Thr Ser Trp Ala Pro Gln Leu Arg Ile Leu Ser His

340 345 350

Glu Ser Val Cys Gly Phe Leu Thr His Cys Gly Ser Gly Ser Ile Val

355 360 365

Glu Gly Leu Met Phe Gly His Pro Leu Ile Met Leu Pro Ile Phe Gly

370 375 380

Asp Gln Pro Leu Asn Ala Arg Leu Leu Glu Asp Lys Gln Val Gly Ile

385 390 395 400

Glu Ile Pro Arg Asn Glu Glu Asp Gly Cys Leu Thr Lys Glu Ser Val

405 410 415

Ala Arg Ser Leu Arg Ser Val Val Val Glu Lys Glu Gly Glu Ile Tyr

420 425 430

Lys Ala Asn Ala Arg Glu Leu Ser Lys Ile Tyr Asn Asp Thr Lys Val

435 440 445

Glu Lys Glu Tyr Val Ser Gln Phe Val Asp Tyr Leu Glu Lys Asn Ala

450 455 460

Arg Ala Val Ala Ile Asp His Glu Ser

465 470

<210> 101

<211> 1377

<212> DNA

<213> Ipomoea purpurea

<220>

<221> CDS

<222> (1)..(1377)

<400> 101

atg ggt tct caa gct acc acc tac cac atg gcc atg tac cca tgg ttc 48

Met Gly Ser Gln Ala Thr Thr Tyr His Met Ala Met Tyr Pro Trp Phe

1 5 10 15

ggt gtc ggt cat ttg act ggt ttc ttc aga ttg gct aac aag ttg gct 96

Gly Val Gly His Leu Thr Gly Phe Phe Arg Leu Ala Asn Lys Leu Ala

20 25 30

ggt aag ggt cac aga atc tct ttc ttg att cca aag aac act caa tcc 144

Gly Lys Gly His Arg Ile Ser Phe Leu Ile Pro Lys Asn Thr Gln Ser

35 40 45

aaa ttg gaa tct ttc aac ttg cac cca cat ttg atc tct ttc gtt cca 192

Lys Leu Glu Ser Phe Asn Leu His Pro His Leu Ile Ser Phe Val Pro

50 55 60

att gtt gtt cca tcc att cca ggt cta cca cca ggt gct gaa acc acc 240

Ile Val Val Pro Ser Ile Pro Gly Leu Pro Pro Gly Ala Glu Thr Thr

65 70 75 80

tct gat gtc cca ttc cca tct acc cat ttg ttg atg gaa gct atg gac 288

Ser Asp Val Pro Phe Pro Ser Thr His Leu Leu Met Glu Ala Met Asp

85 90 95

aag act caa aac gat att gaa atc atc ttg aag gac ttg aag gtc gat 336

Lys Thr Gln Asn Asp Ile Glu Ile Ile Leu Lys Asp Leu Lys Val Asp

100 105 110

gtc gtt ttc tac gat ttc acc cac tgg tta cca tct ttg gcc aga aag 384

Val Val Phe Tyr Asp Phe Thr His Trp Leu Pro Ser Leu Ala Arg Lys

115 120 125

atc ggt atc aaa tcc gtc ttt tac tcc acc att tct cca ttg atg cac 432

Ile Gly Ile Lys Ser Val Phe Tyr Ser Thr Ile Ser Pro Leu Met His

130 135 140

ggt tac gct ttg tcc cca gaa aga aga gtt gtc ggt aag caa ttg act 480

Gly Tyr Ala Leu Ser Pro Glu Arg Arg Val Val Gly Lys Gln Leu Thr

145 150 155 160

gaa gct gac atg atg aag gct cca gct tct ttc cca gac cca tcc atc 528

Glu Ala Asp Met Met Lys Ala Pro Ala Ser Phe Pro Asp Pro Ser Ile

165 170 175

aaa ttg cac gct cac gaa gcc aga ggt ttc acc gcc aga acc gtt atg 576

Lys Leu His Ala His Glu Ala Arg Gly Phe Thr Ala Arg Thr Val Met

180 185 190

aag ttc ggt ggt gat atc act ttc ttc gat cgt att ttc act gct gtt 624

Lys Phe Gly Gly Asp Ile Thr Phe Phe Asp Arg Ile Phe Thr Ala Val

195 200 205

tct gaa tct gat ggt ttg gct tac tcc act tgt cgt gaa atc gaa ggt 672

Ser Glu Ser Asp Gly Leu Ala Tyr Ser Thr Cys Arg Glu Ile Glu Gly

210 215 220

caa ttc tgt gac tac att gaa act caa ttc caa aag cca gtc tta ttg 720

Gln Phe Cys Asp Tyr Ile Glu Thr Gln Phe Gln Lys Pro Val Leu Leu

225 230 235 240

gct ggt cca gct ttg cca gtt cct tcc aag tcc acc atg gaa caa aaa 768

Ala Gly Pro Ala Leu Pro Val Pro Ser Lys Ser Thr Met Glu Gln Lys

245 250 255

tgg tct gac tgg tta ggt aag ttc aag gaa ggt tct gtc att tac tgt 816

Trp Ser Asp Trp Leu Gly Lys Phe Lys Glu Gly Ser Val Ile Tyr Cys

260 265 270

gct ttc ggt tct gaa tgt acc ttg aga aag gac aaa ttc caa gaa cta 864

Ala Phe Gly Ser Glu Cys Thr Leu Arg Lys Asp Lys Phe Gln Glu Leu

275 280 285

tta tgg ggt ttg gaa ttg act ggt atg cca ttc ttt gct gct ttg aag 912

Leu Trp Gly Leu Glu Leu Thr Gly Met Pro Phe Phe Ala Ala Leu Lys

290 295 300

cct cca ttt gaa act gaa tct gtt gaa gct gcc atc cca gaa gaa ttg 960

Pro Pro Phe Glu Thr Glu Ser Val Glu Ala Ala Ile Pro Glu Glu Leu

305 310 315 320

aag gaa aag atc caa ggt aga ggt atc gtt cac ggt gaa tgg gtt caa 1008

Lys Glu Lys Ile Gln Gly Arg Gly Ile Val His Gly Glu Trp Val Gln

325 330 335

caa caa ttg ttt ttg caa cac cca tct gtt ggt tgt ttc gtt tcc cac 1056

Gln Gln Leu Phe Leu Gln His Pro Ser Val Gly Cys Phe Val Ser His

340 345 350

tgt ggt tgg gct tct ttg tcc gaa gct ttg gtc aat gac tgt caa atc 1104

Cys Gly Trp Ala Ser Leu Ser Glu Ala Leu Val Asn Asp Cys Gln Ile

355 360 365

gtc ttg ttg cca caa gtc ggt gac caa att att aac gcc aga atc atg 1152

Val Leu Leu Pro Gln Val Gly Asp Gln Ile Ile Asn Ala Arg Ile Met

370 375 380

tcc gtt tct ttg aag gtt ggt gtt gaa gtc gaa aag ggt gaa gaa gat 1200

Ser Val Ser Leu Lys Val Gly Val Glu Val Glu Lys Gly Glu Glu Asp

385 390 395 400

ggt gtt ttc tcc aga gaa tct gtc tgt aag gcc gtc aag gct gtc atg 1248

Gly Val Phe Ser Arg Glu Ser Val Cys Lys Ala Val Lys Ala Val Met

405 410 415

gat gaa aag tct gaa atc ggt aga gaa gtt aga ggt aac cac gac aaa 1296

Asp Glu Lys Ser Glu Ile Gly Arg Glu Val Arg Gly Asn His Asp Lys

420 425 430

ttg aga ggt ttc ttg atg aac gct gac ttg gac tcc aag tac atg gac 1344

Leu Arg Gly Phe Leu Met Asn Ala Asp Leu Asp Ser Lys Tyr Met Asp

435 440 445

tct ttc aac caa aag ttg caa gac tta tta ggg 1377

Ser Phe Asn Gln Lys Leu Gln Asp Leu Leu Gly

450 455

<210> 102

<211> 459

<212> PRT

<213> Ipomoea purpurea

<400> 102

Met Gly Ser Gln Ala Thr Thr Tyr His Met Ala Met Tyr Pro Trp Phe

1 5 10 15

Gly Val Gly His Leu Thr Gly Phe Phe Arg Leu Ala Asn Lys Leu Ala

20 25 30

Gly Lys Gly His Arg Ile Ser Phe Leu Ile Pro Lys Asn Thr Gln Ser

35 40 45

Lys Leu Glu Ser Phe Asn Leu His Pro His Leu Ile Ser Phe Val Pro

50 55 60

Ile Val Val Pro Ser Ile Pro Gly Leu Pro Pro Gly Ala Glu Thr Thr

65 70 75 80

Ser Asp Val Pro Phe Pro Ser Thr His Leu Leu Met Glu Ala Met Asp

85 90 95

Lys Thr Gln Asn Asp Ile Glu Ile Ile Leu Lys Asp Leu Lys Val Asp

100 105 110

Val Val Phe Tyr Asp Phe Thr His Trp Leu Pro Ser Leu Ala Arg Lys

115 120 125

Ile Gly Ile Lys Ser Val Phe Tyr Ser Thr Ile Ser Pro Leu Met His

130 135 140

Gly Tyr Ala Leu Ser Pro Glu Arg Arg Val Val Gly Lys Gln Leu Thr

145 150 155 160

Glu Ala Asp Met Met Lys Ala Pro Ala Ser Phe Pro Asp Pro Ser Ile

165 170 175

Lys Leu His Ala His Glu Ala Arg Gly Phe Thr Ala Arg Thr Val Met

180 185 190

Lys Phe Gly Gly Asp Ile Thr Phe Phe Asp Arg Ile Phe Thr Ala Val

195 200 205

Ser Glu Ser Asp Gly Leu Ala Tyr Ser Thr Cys Arg Glu Ile Glu Gly

210 215 220

Gln Phe Cys Asp Tyr Ile Glu Thr Gln Phe Gln Lys Pro Val Leu Leu

225 230 235 240

Ala Gly Pro Ala Leu Pro Val Pro Ser Lys Ser Thr Met Glu Gln Lys

245 250 255

Trp Ser Asp Trp Leu Gly Lys Phe Lys Glu Gly Ser Val Ile Tyr Cys

260 265 270

Ala Phe Gly Ser Glu Cys Thr Leu Arg Lys Asp Lys Phe Gln Glu Leu

275 280 285

Leu Trp Gly Leu Glu Leu Thr Gly Met Pro Phe Phe Ala Ala Leu Lys

290 295 300

Pro Pro Phe Glu Thr Glu Ser Val Glu Ala Ala Ile Pro Glu Glu Leu

305 310 315 320

Lys Glu Lys Ile Gln Gly Arg Gly Ile Val His Gly Glu Trp Val Gln

325 330 335

Gln Gln Leu Phe Leu Gln His Pro Ser Val Gly Cys Phe Val Ser His

340 345 350

Cys Gly Trp Ala Ser Leu Ser Glu Ala Leu Val Asn Asp Cys Gln Ile

355 360 365

Val Leu Leu Pro Gln Val Gly Asp Gln Ile Ile Asn Ala Arg Ile Met

370 375 380

Ser Val Ser Leu Lys Val Gly Val Glu Val Glu Lys Gly Glu Glu Asp

385 390 395 400

Gly Val Phe Ser Arg Glu Ser Val Cys Lys Ala Val Lys Ala Val Met

405 410 415

Asp Glu Lys Ser Glu Ile Gly Arg Glu Val Arg Gly Asn His Asp Lys

420 425 430

Leu Arg Gly Phe Leu Met Asn Ala Asp Leu Asp Ser Lys Tyr Met Asp

435 440 445

Ser Phe Asn Gln Lys Leu Gln Asp Leu Leu Gly

450 455

<210> 103

<211> 1314

<212> DNA

<213> Bellis perennis

<220>

<221> CDS

<222> (1)..(1314)

<400> 103

atg gac tct aag att gac tcc aag act ttc aga gtt gtt atg ttg cca 48

Met Asp Ser Lys Ile Asp Ser Lys Thr Phe Arg Val Val Met Leu Pro

1 5 10 15

tgg ttg gct tac tcc cac atc tct tct ttc ttg gtt ttc gct aag aga 96

Trp Leu Ala Tyr Ser His Ile Ser Ser Phe Leu Val Phe Ala Lys Arg

20 25 30

ttg acc aac cac aac ttc cac atc tac atc tgt tcc tct caa acc aac 144

Leu Thr Asn His Asn Phe His Ile Tyr Ile Cys Ser Ser Gln Thr Asn

35 40 45

atg caa tac ttg aag aac aac ttg act tct caa tac tcc aag tcc atc 192

Met Gln Tyr Leu Lys Asn Asn Leu Thr Ser Gln Tyr Ser Lys Ser Ile

50 55 60

caa ttg att gaa ttg aac ttg cca tct tct tct gaa ttg cca ttg caa 240

Gln Leu Ile Glu Leu Asn Leu Pro Ser Ser Ser Glu Leu Pro Leu Gln

65 70 75 80

tac cac acc acc cac ggt cta cca cct cat ttg acc aag act cta tcc 288

Tyr His Thr Thr His Gly Leu Pro Pro His Leu Thr Lys Thr Leu Ser

85 90 95

gat gac tac caa aaa tct ggt cca gac ttc gaa acc atc ttg atc aaa 336

Asp Asp Tyr Gln Lys Ser Gly Pro Asp Phe Glu Thr Ile Leu Ile Lys

100 105 110

ttg aac cct cac tta gtc atc tac gac ttc aac caa tta tgg gct cca 384

Leu Asn Pro His Leu Val Ile Tyr Asp Phe Asn Gln Leu Trp Ala Pro

115 120 125

gaa gtt gct tcc act ttg cac att cca tct atc caa ttg ttg tcc ggt 432

Glu Val Ala Ser Thr Leu His Ile Pro Ser Ile Gln Leu Leu Ser Gly

130 135 140

tgt gtt gct ttg tac gct ttg gat gct cat ttg tac acc aag cca ttg 480

Cys Val Ala Leu Tyr Ala Leu Asp Ala His Leu Tyr Thr Lys Pro Leu

145 150 155 160

gat gaa aac ttg gcc aag ttc cca ttc cca gaa atc tac cca aag aac 528

Asp Glu Asn Leu Ala Lys Phe Pro Phe Pro Glu Ile Tyr Pro Lys Asn

165 170 175

aga gat atc cca aag ggt ggt tcc aaa tac atc gaa aga ttt gtc gac 576

Arg Asp Ile Pro Lys Gly Gly Ser Lys Tyr Ile Glu Arg Phe Val Asp

180 185 190

tgt atg aga aga tct tgt gaa atc atc ttg gtc aga tcc act atg gaa 624

Cys Met Arg Arg Ser Cys Glu Ile Ile Leu Val Arg Ser Thr Met Glu

195 200 205

ttg gaa ggt aag tac att gac tac ttg tct aag act tta ggt aag aag 672

Leu Glu Gly Lys Tyr Ile Asp Tyr Leu Ser Lys Thr Leu Gly Lys Lys

210 215 220

gtc tta cca gtt ggt cca tta gtc caa gaa gct tct ttg ttg caa gat 720

Val Leu Pro Val Gly Pro Leu Val Gln Glu Ala Ser Leu Leu Gln Asp

225 230 235 240

gac cac atc tgg atc atg aaa tgg ttg gac aag aag gaa gaa tcc tct 768

Asp His Ile Trp Ile Met Lys Trp Leu Asp Lys Lys Glu Glu Ser Ser

245 250 255

gtt gtc ttt gtt tgt ttc ggt tct gaa tac att ttg tct gac aac gaa 816

Val Val Phe Val Cys Phe Gly Ser Glu Tyr Ile Leu Ser Asp Asn Glu

260 265 270

atc gaa gac att gct tac ggt ttg gaa ttg tct caa gtc tct ttc gtc 864

Ile Glu Asp Ile Ala Tyr Gly Leu Glu Leu Ser Gln Val Ser Phe Val

275 280 285

tgg gct atc aga gcc aag acc tct gct ttg aat ggt ttc att gac cgt 912

Trp Ala Ile Arg Ala Lys Thr Ser Ala Leu Asn Gly Phe Ile Asp Arg

290 295 300

gtt ggt gac aag ggt ttg gtc att gac aaa tgg gtt cca caa gct aac 960

Val Gly Asp Lys Gly Leu Val Ile Asp Lys Trp Val Pro Gln Ala Asn

305 310 315 320

atc ttg tcc cac tct tcc act ggt ggt ttc atc tcc cac tgt ggt tgg 1008

Ile Leu Ser His Ser Ser Thr Gly Gly Phe Ile Ser His Cys Gly Trp

325 330 335

tcc tct acc atg gaa tcc atc aga tac ggt gtt cca atc att gct atg 1056

Ser Ser Thr Met Glu Ser Ile Arg Tyr Gly Val Pro Ile Ile Ala Met

340 345 350

cca atg caa ttc gat caa cca tac aac gcc aga ttg atg gaa act gtc 1104

Pro Met Gln Phe Asp Gln Pro Tyr Asn Ala Arg Leu Met Glu Thr Val

355 360 365

ggt gct ggt att gaa gtt ggt aga gat ggt gaa ggt aga ttg aag cgt 1152

Gly Ala Gly Ile Glu Val Gly Arg Asp Gly Glu Gly Arg Leu Lys Arg

370 375 380

gaa gaa att gcc gct gtt gtc aga aag gtt gtt gtt gaa gat tct ggt 1200

Glu Glu Ile Ala Ala Val Val Arg Lys Val Val Val Glu Asp Ser Gly

385 390 395 400

gaa tct atc aga gaa aag gcc aag gaa tta ggt gaa att atg aag aag 1248

Glu Ser Ile Arg Glu Lys Ala Lys Glu Leu Gly Glu Ile Met Lys Lys

405 410 415

aac atg gaa gct gaa gtc gat ggt att gtc att gaa aac tta gtc aaa 1296

Asn Met Glu Ala Glu Val Asp Gly Ile Val Ile Glu Asn Leu Val Lys

420 425 430

ttg tgt gaa atg aac aac 1314

Leu Cys Glu Met Asn Asn

435

<210> 104

<211> 438

<212> PRT

<213> Bellis perennis

<400> 104

Met Asp Ser Lys Ile Asp Ser Lys Thr Phe Arg Val Val Met Leu Pro

1 5 10 15

Trp Leu Ala Tyr Ser His Ile Ser Ser Phe Leu Val Phe Ala Lys Arg

20 25 30

Leu Thr Asn His Asn Phe His Ile Tyr Ile Cys Ser Ser Gln Thr Asn

35 40 45

Met Gln Tyr Leu Lys Asn Asn Leu Thr Ser Gln Tyr Ser Lys Ser Ile

50 55 60

Gln Leu Ile Glu Leu Asn Leu Pro Ser Ser Ser Glu Leu Pro Leu Gln

65 70 75 80

Tyr His Thr Thr His Gly Leu Pro Pro His Leu Thr Lys Thr Leu Ser

85 90 95

Asp Asp Tyr Gln Lys Ser Gly Pro Asp Phe Glu Thr Ile Leu Ile Lys

100 105 110

Leu Asn Pro His Leu Val Ile Tyr Asp Phe Asn Gln Leu Trp Ala Pro

115 120 125

Glu Val Ala Ser Thr Leu His Ile Pro Ser Ile Gln Leu Leu Ser Gly

130 135 140

Cys Val Ala Leu Tyr Ala Leu Asp Ala His Leu Tyr Thr Lys Pro Leu

145 150 155 160

Asp Glu Asn Leu Ala Lys Phe Pro Phe Pro Glu Ile Tyr Pro Lys Asn

165 170 175

Arg Asp Ile Pro Lys Gly Gly Ser Lys Tyr Ile Glu Arg Phe Val Asp

180 185 190

Cys Met Arg Arg Ser Cys Glu Ile Ile Leu Val Arg Ser Thr Met Glu

195 200 205

Leu Glu Gly Lys Tyr Ile Asp Tyr Leu Ser Lys Thr Leu Gly Lys Lys

210 215 220

Val Leu Pro Val Gly Pro Leu Val Gln Glu Ala Ser Leu Leu Gln Asp

225 230 235 240

Asp His Ile Trp Ile Met Lys Trp Leu Asp Lys Lys Glu Glu Ser Ser

245 250 255

Val Val Phe Val Cys Phe Gly Ser Glu Tyr Ile Leu Ser Asp Asn Glu

260 265 270

Ile Glu Asp Ile Ala Tyr Gly Leu Glu Leu Ser Gln Val Ser Phe Val

275 280 285

Trp Ala Ile Arg Ala Lys Thr Ser Ala Leu Asn Gly Phe Ile Asp Arg

290 295 300

Val Gly Asp Lys Gly Leu Val Ile Asp Lys Trp Val Pro Gln Ala Asn

305 310 315 320

Ile Leu Ser His Ser Ser Thr Gly Gly Phe Ile Ser His Cys Gly Trp

325 330 335

Ser Ser Thr Met Glu Ser Ile Arg Tyr Gly Val Pro Ile Ile Ala Met

340 345 350

Pro Met Gln Phe Asp Gln Pro Tyr Asn Ala Arg Leu Met Glu Thr Val

355 360 365

Gly Ala Gly Ile Glu Val Gly Arg Asp Gly Glu Gly Arg Leu Lys Arg

370 375 380

Glu Glu Ile Ala Ala Val Val Arg Lys Val Val Val Glu Asp Ser Gly

385 390 395 400

Glu Ser Ile Arg Glu Lys Ala Lys Glu Leu Gly Glu Ile Met Lys Lys

405 410 415

Asn Met Glu Ala Glu Val Asp Gly Ile Val Ile Glu Asn Leu Val Lys

420 425 430

Leu Cys Glu Met Asn Asn

435

<210> 105

<211> 1419

<212> DNA

<213> Stevia rebaudiana

<220>

<221> CDS

<222> (1)..(1419)

<400> 105

atg gcc act tct gac tct att gtc gat gac aga aag caa ttg cac gtt 48

Met Ala Thr Ser Asp Ser Ile Val Asp Asp Arg Lys Gln Leu His Val

1 5 10 15

gcc act ttc cca tgg ttg gcc ttc ggt cac att ttg cca tac ttg caa 96

Ala Thr Phe Pro Trp Leu Ala Phe Gly His Ile Leu Pro Tyr Leu Gln

20 25 30

ttg tcc aag ttg att gct gaa aag ggt cac aag gtt tct ttc ttg tcc 144

Leu Ser Lys Leu Ile Ala Glu Lys Gly His Lys Val Ser Phe Leu Ser

35 40 45

act acc aga aac atc caa aga tta tct tct cac atc tct cca tta atc 192

Thr Thr Arg Asn Ile Gln Arg Leu Ser Ser His Ile Ser Pro Leu Ile

50 55 60

aac gtt gtc caa ttg act tta cca aga gtt caa gaa ttg cca gaa gat 240

Asn Val Val Gln Leu Thr Leu Pro Arg Val Gln Glu Leu Pro Glu Asp

65 70 75 80

gct gaa gct acc acc gat gtc cat cca gaa gat atc cca tac ttg aag 288

Ala Glu Ala Thr Thr Asp Val His Pro Glu Asp Ile Pro Tyr Leu Lys

85 90 95

aag gct tct gac ggt ttg caa cct gaa gtc acc cgt ttc ttg gaa caa 336

Lys Ala Ser Asp Gly Leu Gln Pro Glu Val Thr Arg Phe Leu Glu Gln

100 105 110

cac tct cca gac tgg atc atc tac gac tac act cac tac tgg tta cct 384

His Ser Pro Asp Trp Ile Ile Tyr Asp Tyr Thr His Tyr Trp Leu Pro

115 120 125

tcc att gct gct tct ttg ggt atc tcc cgt gct cat ttc tcc gtc acc 432

Ser Ile Ala Ala Ser Leu Gly Ile Ser Arg Ala His Phe Ser Val Thr

130 135 140

act cca tgg gct att gct tac atg ggt cca tct gct gat gct atg atc 480

Thr Pro Trp Ala Ile Ala Tyr Met Gly Pro Ser Ala Asp Ala Met Ile

145 150 155 160

aac ggt tct gat ggt aga acc act gtt gaa gat ttg acc act cca cca 528

Asn Gly Ser Asp Gly Arg Thr Thr Val Glu Asp Leu Thr Thr Pro Pro

165 170 175

aaa tgg ttc cca ttc cca act aag gtc tgt tgg aga aag cac gat ttg 576

Lys Trp Phe Pro Phe Pro Thr Lys Val Cys Trp Arg Lys His Asp Leu

180 185 190

gcc aga ttg gtt cca tac aag gct cca ggt atc tct gat ggt tac aga 624

Ala Arg Leu Val Pro Tyr Lys Ala Pro Gly Ile Ser Asp Gly Tyr Arg

195 200 205

atg ggt ttg gtt ttg aaa ggt tct gac tgt ttg ttg tcc aaa tgt tac 672

Met Gly Leu Val Leu Lys Gly Ser Asp Cys Leu Leu Ser Lys Cys Tyr

210 215 220

cat gaa ttc ggt act caa tgg tta cca ttg ttg gaa act ttg cac caa 720

His Glu Phe Gly Thr Gln Trp Leu Pro Leu Leu Glu Thr Leu His Gln

225 230 235 240

gtt cca gtt gtt cca gtc ggt cta tta cca cca gaa gtc cca ggt gac 768

Val Pro Val Val Pro Val Gly Leu Leu Pro Pro Glu Val Pro Gly Asp

245 250 255

gaa aag gac gaa acc tgg gtt tcc atc aag aaa tgg tta gat ggt aag 816

Glu Lys Asp Glu Thr Trp Val Ser Ile Lys Lys Trp Leu Asp Gly Lys

260 265 270

caa aag ggt tcc gtt gtc tac gtt gct ttg ggt tct gaa gtc ttg gtt 864

Gln Lys Gly Ser Val Val Tyr Val Ala Leu Gly Ser Glu Val Leu Val

275 280 285

tct caa act gaa gtt gtc gaa ttg gct tta ggt ttg gaa ttg tcc ggt 912

Ser Gln Thr Glu Val Val Glu Leu Ala Leu Gly Leu Glu Leu Ser Gly

290 295 300

ttg cct ttc gtc tgg gct tac aga aag cca aag ggt cca gct aaa tct 960

Leu Pro Phe Val Trp Ala Tyr Arg Lys Pro Lys Gly Pro Ala Lys Ser

305 310 315 320

gac tcc gtc gaa tta cca gac ggt ttc gtt gaa aga acc cgt gac aga 1008

Asp Ser Val Glu Leu Pro Asp Gly Phe Val Glu Arg Thr Arg Asp Arg

325 330 335

ggt ttg gtc tgg act tcc tgg gct cca caa ttg aga atc ttg tcc cac 1056

Gly Leu Val Trp Thr Ser Trp Ala Pro Gln Leu Arg Ile Leu Ser His

340 345 350

gaa tct gtt tgt ggt ttc ttg acc cac tgt ggt tct ggt tcc att gtc 1104

Glu Ser Val Cys Gly Phe Leu Thr His Cys Gly Ser Gly Ser Ile Val

355 360 365

gaa ggt ttg atg ttt ggt cac cca ttg atc atg ttg cca atc ttc ggt 1152

Glu Gly Leu Met Phe Gly His Pro Leu Ile Met Leu Pro Ile Phe Gly

370 375 380

gac caa cca ttg aac gcc aga tta ttg gaa gac aag caa gtc ggt att 1200

Asp Gln Pro Leu Asn Ala Arg Leu Leu Glu Asp Lys Gln Val Gly Ile

385 390 395 400

gaa att cca aga aac gaa gaa gat ggt tgt ttg acc aag gaa tct gtt 1248

Glu Ile Pro Arg Asn Glu Glu Asp Gly Cys Leu Thr Lys Glu Ser Val

405 410 415

gcc aga tct ttg aga tcc gtt gtt gtc gaa aag gaa ggt gaa atc tac 1296

Ala Arg Ser Leu Arg Ser Val Val Val Glu Lys Glu Gly Glu Ile Tyr

420 425 430

aag gct aac gct aga gaa ttg tct aag atc tac aac gac acc aag gtt 1344

Lys Ala Asn Ala Arg Glu Leu Ser Lys Ile Tyr Asn Asp Thr Lys Val

435 440 445

gaa aag gaa tac gtt tct caa ttt gtc gac tac ttg gaa aag aac acc 1392

Glu Lys Glu Tyr Val Ser Gln Phe Val Asp Tyr Leu Glu Lys Asn Thr

450 455 460

aga gct gtt gcc att gac cac gaa agt 1419

Arg Ala Val Ala Ile Asp His Glu Ser

465 470

<210> 106

<211> 473

<212> PRT

<213> Stevia rebaudiana

<400> 106

Met Ala Thr Ser Asp Ser Ile Val Asp Asp Arg Lys Gln Leu His Val

1 5 10 15

Ala Thr Phe Pro Trp Leu Ala Phe Gly His Ile Leu Pro Tyr Leu Gln

20 25 30

Leu Ser Lys Leu Ile Ala Glu Lys Gly His Lys Val Ser Phe Leu Ser

35 40 45

Thr Thr Arg Asn Ile Gln Arg Leu Ser Ser His Ile Ser Pro Leu Ile

50 55 60

Asn Val Val Gln Leu Thr Leu Pro Arg Val Gln Glu Leu Pro Glu Asp

65 70 75 80

Ala Glu Ala Thr Thr Asp Val His Pro Glu Asp Ile Pro Tyr Leu Lys

85 90 95

Lys Ala Ser Asp Gly Leu Gln Pro Glu Val Thr Arg Phe Leu Glu Gln

100 105 110

His Ser Pro Asp Trp Ile Ile Tyr Asp Tyr Thr His Tyr Trp Leu Pro

115 120 125

Ser Ile Ala Ala Ser Leu Gly Ile Ser Arg Ala His Phe Ser Val Thr

130 135 140

Thr Pro Trp Ala Ile Ala Tyr Met Gly Pro Ser Ala Asp Ala Met Ile

145 150 155 160

Asn Gly Ser Asp Gly Arg Thr Thr Val Glu Asp Leu Thr Thr Pro Pro

165 170 175

Lys Trp Phe Pro Phe Pro Thr Lys Val Cys Trp Arg Lys His Asp Leu

180 185 190

Ala Arg Leu Val Pro Tyr Lys Ala Pro Gly Ile Ser Asp Gly Tyr Arg

195 200 205

Met Gly Leu Val Leu Lys Gly Ser Asp Cys Leu Leu Ser Lys Cys Tyr

210 215 220

His Glu Phe Gly Thr Gln Trp Leu Pro Leu Leu Glu Thr Leu His Gln

225 230 235 240

Val Pro Val Val Pro Val Gly Leu Leu Pro Pro Glu Val Pro Gly Asp

245 250 255

Glu Lys Asp Glu Thr Trp Val Ser Ile Lys Lys Trp Leu Asp Gly Lys

260 265 270

Gln Lys Gly Ser Val Val Tyr Val Ala Leu Gly Ser Glu Val Leu Val

275 280 285

Ser Gln Thr Glu Val Val Glu Leu Ala Leu Gly Leu Glu Leu Ser Gly

290 295 300

Leu Pro Phe Val Trp Ala Tyr Arg Lys Pro Lys Gly Pro Ala Lys Ser

305 310 315 320

Asp Ser Val Glu Leu Pro Asp Gly Phe Val Glu Arg Thr Arg Asp Arg

325 330 335

Gly Leu Val Trp Thr Ser Trp Ala Pro Gln Leu Arg Ile Leu Ser His

340 345 350

Glu Ser Val Cys Gly Phe Leu Thr His Cys Gly Ser Gly Ser Ile Val

355 360 365

Glu Gly Leu Met Phe Gly His Pro Leu Ile Met Leu Pro Ile Phe Gly

370 375 380

Asp Gln Pro Leu Asn Ala Arg Leu Leu Glu Asp Lys Gln Val Gly Ile

385 390 395 400

Glu Ile Pro Arg Asn Glu Glu Asp Gly Cys Leu Thr Lys Glu Ser Val

405 410 415

Ala Arg Ser Leu Arg Ser Val Val Val Glu Lys Glu Gly Glu Ile Tyr

420 425 430

Lys Ala Asn Ala Arg Glu Leu Ser Lys Ile Tyr Asn Asp Thr Lys Val

435 440 445

Glu Lys Glu Tyr Val Ser Gln Phe Val Asp Tyr Leu Glu Lys Asn Thr

450 455 460

Arg Ala Val Ala Ile Asp His Glu Ser

465 470

<210> 107

<211> 1455

<212> DNA

<213> Stevia rebaudiana

<220>

<221> CDS

<222> (1)..(1455)

<400> 107

atg tac aac gtc acc tac cat caa aac tcc aag gct atg gct act tct 48

Met Tyr Asn Val Thr Tyr His Gln Asn Ser Lys Ala Met Ala Thr Ser

1 5 10 15

gac tct att gtc gat gac aga aag caa ttg cac gtt gcc act ttc cca 96

Asp Ser Ile Val Asp Asp Arg Lys Gln Leu His Val Ala Thr Phe Pro

20 25 30

tgg tta gct ttc ggt cac atc tta cca ttc ttg caa ttg tcc aaa ttg 144

Trp Leu Ala Phe Gly His Ile Leu Pro Phe Leu Gln Leu Ser Lys Leu

35 40 45

att gct gaa aag ggt cac aag gtt tct ttc ttg tct acc act aga aac 192

Ile Ala Glu Lys Gly His Lys Val Ser Phe Leu Ser Thr Thr Arg Asn

50 55 60

att caa aga ttg tct tcc cac atc tct cca ttg atc aac gtt gtt caa 240

Ile Gln Arg Leu Ser Ser His Ile Ser Pro Leu Ile Asn Val Val Gln

65 70 75 80

ttg act cta cca aga gtc caa gaa tta cca gaa gat gct gaa gct acc 288

Leu Thr Leu Pro Arg Val Gln Glu Leu Pro Glu Asp Ala Glu Ala Thr

85 90 95

acc gat gtc cat cca gaa gat atc caa tac ttg aag aag gct gtt gac 336

Thr Asp Val His Pro Glu Asp Ile Gln Tyr Leu Lys Lys Ala Val Asp

100 105 110

ggt ttg caa cca gaa gtt acc aga ttc ttg gaa caa cac tct cca gac 384

Gly Leu Gln Pro Glu Val Thr Arg Phe Leu Glu Gln His Ser Pro Asp

115 120 125

tgg atc atc tac gac ttc acc cac tac tgg tta cct tcc att gct gcc 432

Trp Ile Ile Tyr Asp Phe Thr His Tyr Trp Leu Pro Ser Ile Ala Ala

130 135 140

tcc ttg ggt atc tcc aga gct tac ttc tgt gtt atc act cca tgg acc 480

Ser Leu Gly Ile Ser Arg Ala Tyr Phe Cys Val Ile Thr Pro Trp Thr

145 150 155 160

att gct tac ttg gct cca tct tct gat gct atg atc aac gac tct gac 528

Ile Ala Tyr Leu Ala Pro Ser Ser Asp Ala Met Ile Asn Asp Ser Asp

165 170 175

ggt aga acc act gtc gaa gat ttg acc act cca cct aaa tgg ttc cca 576

Gly Arg Thr Thr Val Glu Asp Leu Thr Thr Pro Pro Lys Trp Phe Pro

180 185 190

ttc cca act aag gtc tgt tgg aga aag cat gac ttg gcc aga atg gaa 624

Phe Pro Thr Lys Val Cys Trp Arg Lys His Asp Leu Ala Arg Met Glu

195 200 205

cca tac gaa gct cca ggt atc tcc gat ggt tac aga atg ggt atg gtt 672

Pro Tyr Glu Ala Pro Gly Ile Ser Asp Gly Tyr Arg Met Gly Met Val

210 215 220

ttc aag ggt tct gac tgt ttg ttg ttc aag tgt tac cac gaa ttc ggt 720

Phe Lys Gly Ser Asp Cys Leu Leu Phe Lys Cys Tyr His Glu Phe Gly

225 230 235 240

act caa tgg tta cca ttg ttg gaa act ttg cac caa gtc cca gtt gtc 768

Thr Gln Trp Leu Pro Leu Leu Glu Thr Leu His Gln Val Pro Val Val

245 250 255

cca gtc ggt ttg ttg cca cca gaa atc cca ggt gac gaa aag gac gaa 816

Pro Val Gly Leu Leu Pro Pro Glu Ile Pro Gly Asp Glu Lys Asp Glu

260 265 270

acc tgg gtt tcc atc aag aaa tgg ttg gat ggt aag caa aag ggt tcc 864

Thr Trp Val Ser Ile Lys Lys Trp Leu Asp Gly Lys Gln Lys Gly Ser

275 280 285

gtt gtc tac gtt gcc ttg ggt tct gaa gct tta gtc tct caa act gaa 912

Val Val Tyr Val Ala Leu Gly Ser Glu Ala Leu Val Ser Gln Thr Glu

290 295 300

gtt gtt gaa ttg gct ttg ggt ttg gaa ttg tct ggt cta cca ttt gtc 960

Val Val Glu Leu Ala Leu Gly Leu Glu Leu Ser Gly Leu Pro Phe Val

305 310 315 320

tgg gct tac aga aag cca aag ggt cca gcc aaa tct gac tcc gtt gaa 1008

Trp Ala Tyr Arg Lys Pro Lys Gly Pro Ala Lys Ser Asp Ser Val Glu

325 330 335

ttg cca gat ggt ttc gtc gaa cgt acc aga gac aga ggt tta gtc tgg 1056

Leu Pro Asp Gly Phe Val Glu Arg Thr Arg Asp Arg Gly Leu Val Trp

340 345 350

act tcc tgg gct cct caa ttg aga atc ttg tct cac gaa tct gtc tgt 1104

Thr Ser Trp Ala Pro Gln Leu Arg Ile Leu Ser His Glu Ser Val Cys

355 360 365

ggt ttc ttg act cac tgt ggt tct ggt tcc att gtc gaa ggt ttg atg 1152

Gly Phe Leu Thr His Cys Gly Ser Gly Ser Ile Val Glu Gly Leu Met

370 375 380

ttc ggt cac cca ttg att atg ttg cca att ttc tgt gac caa cca ttg 1200

Phe Gly His Pro Leu Ile Met Leu Pro Ile Phe Cys Asp Gln Pro Leu

385 390 395 400

aac gct aga tta ttg gaa gac aaa caa gtc ggt att gaa atc cca aga 1248

Asn Ala Arg Leu Leu Glu Asp Lys Gln Val Gly Ile Glu Ile Pro Arg

405 410 415

aac gaa gaa gat ggt tgt ttg acc aag gaa tct gtt gct cgt tct ttg 1296

Asn Glu Glu Asp Gly Cys Leu Thr Lys Glu Ser Val Ala Arg Ser Leu

420 425 430

aga tct gtt gtt gtc gaa aac gaa ggt gaa atc tac aag gcc aat gct 1344

Arg Ser Val Val Val Glu Asn Glu Gly Glu Ile Tyr Lys Ala Asn Ala

435 440 445

cgt gct ttg tcc aag atc tac aac gat acc aag gtt gaa aag gaa tac 1392

Arg Ala Leu Ser Lys Ile Tyr Asn Asp Thr Lys Val Glu Lys Glu Tyr

450 455 460

gtt tct caa ttt gtt gac tac ttg gaa aag aac gcc aga gct gtt gcc 1440

Val Ser Gln Phe Val Asp Tyr Leu Glu Lys Asn Ala Arg Ala Val Ala

465 470 475 480

att gac cac gaa tcc 1455

Ile Asp His Glu Ser

485

<210> 108

<211> 485

<212> PRT

<213> Stevia rebaudiana

<400> 108

Met Tyr Asn Val Thr Tyr His Gln Asn Ser Lys Ala Met Ala Thr Ser

1 5 10 15

Asp Ser Ile Val Asp Asp Arg Lys Gln Leu His Val Ala Thr Phe Pro

20 25 30

Trp Leu Ala Phe Gly His Ile Leu Pro Phe Leu Gln Leu Ser Lys Leu

35 40 45

Ile Ala Glu Lys Gly His Lys Val Ser Phe Leu Ser Thr Thr Arg Asn

50 55 60

Ile Gln Arg Leu Ser Ser His Ile Ser Pro Leu Ile Asn Val Val Gln

65 70 75 80

Leu Thr Leu Pro Arg Val Gln Glu Leu Pro Glu Asp Ala Glu Ala Thr

85 90 95

Thr Asp Val His Pro Glu Asp Ile Gln Tyr Leu Lys Lys Ala Val Asp

100 105 110

Gly Leu Gln Pro Glu Val Thr Arg Phe Leu Glu Gln His Ser Pro Asp

115 120 125

Trp Ile Ile Tyr Asp Phe Thr His Tyr Trp Leu Pro Ser Ile Ala Ala

130 135 140

Ser Leu Gly Ile Ser Arg Ala Tyr Phe Cys Val Ile Thr Pro Trp Thr

145 150 155 160

Ile Ala Tyr Leu Ala Pro Ser Ser Asp Ala Met Ile Asn Asp Ser Asp

165 170 175

Gly Arg Thr Thr Val Glu Asp Leu Thr Thr Pro Pro Lys Trp Phe Pro

180 185 190

Phe Pro Thr Lys Val Cys Trp Arg Lys His Asp Leu Ala Arg Met Glu

195 200 205

Pro Tyr Glu Ala Pro Gly Ile Ser Asp Gly Tyr Arg Met Gly Met Val

210 215 220

Phe Lys Gly Ser Asp Cys Leu Leu Phe Lys Cys Tyr His Glu Phe Gly

225 230 235 240

Thr Gln Trp Leu Pro Leu Leu Glu Thr Leu His Gln Val Pro Val Val

245 250 255

Pro Val Gly Leu Leu Pro Pro Glu Ile Pro Gly Asp Glu Lys Asp Glu

260 265 270

Thr Trp Val Ser Ile Lys Lys Trp Leu Asp Gly Lys Gln Lys Gly Ser

275 280 285

Val Val Tyr Val Ala Leu Gly Ser Glu Ala Leu Val Ser Gln Thr Glu

290 295 300

Val Val Glu Leu Ala Leu Gly Leu Glu Leu Ser Gly Leu Pro Phe Val

305 310 315 320

Trp Ala Tyr Arg Lys Pro Lys Gly Pro Ala Lys Ser Asp Ser Val Glu

325 330 335

Leu Pro Asp Gly Phe Val Glu Arg Thr Arg Asp Arg Gly Leu Val Trp

340 345 350

Thr Ser Trp Ala Pro Gln Leu Arg Ile Leu Ser His Glu Ser Val Cys

355 360 365

Gly Phe Leu Thr His Cys Gly Ser Gly Ser Ile Val Glu Gly Leu Met

370 375 380

Phe Gly His Pro Leu Ile Met Leu Pro Ile Phe Cys Asp Gln Pro Leu

385 390 395 400

Asn Ala Arg Leu Leu Glu Asp Lys Gln Val Gly Ile Glu Ile Pro Arg

405 410 415

Asn Glu Glu Asp Gly Cys Leu Thr Lys Glu Ser Val Ala Arg Ser Leu

420 425 430

Arg Ser Val Val Val Glu Asn Glu Gly Glu Ile Tyr Lys Ala Asn Ala

435 440 445

Arg Ala Leu Ser Lys Ile Tyr Asn Asp Thr Lys Val Glu Lys Glu Tyr

450 455 460

Val Ser Gln Phe Val Asp Tyr Leu Glu Lys Asn Ala Arg Ala Val Ala

465 470 475 480

Ile Asp His Glu Ser

485

<210> 109

<211> 1419

<212> DNA

<213> Stevia rebaudiana

<220>

<221> CDS

<222> (1)..(1419)

<400> 109

atg gcc act tct gac tcc att gtt gat gac aga aag caa ttg cac gtt 48

Met Ala Thr Ser Asp Ser Ile Val Asp Asp Arg Lys Gln Leu His Val

1 5 10 15

gct act ttc cca tgg ttg gcc ttt ggt cac att ttg cct ttc ttg caa 96

Ala Thr Phe Pro Trp Leu Ala Phe Gly His Ile Leu Pro Phe Leu Gln

20 25 30

ttg tct aaa ttg att gct gaa aag ggt cac aag gtt tcc ttc ttg tct 144

Leu Ser Lys Leu Ile Ala Glu Lys Gly His Lys Val Ser Phe Leu Ser

35 40 45

acc acc aga aac atc caa aga tta tct tcc cac atc tct cct ttg atc 192

Thr Thr Arg Asn Ile Gln Arg Leu Ser Ser His Ile Ser Pro Leu Ile

50 55 60

aac gtt gtt caa ttg act cta cca cgt gtc caa gaa tta cca gaa gac 240

Asn Val Val Gln Leu Thr Leu Pro Arg Val Gln Glu Leu Pro Glu Asp

65 70 75 80

gct gaa gct act acc gat gtc cac cca gaa gat atc caa tac ttg aag 288

Ala Glu Ala Thr Thr Asp Val His Pro Glu Asp Ile Gln Tyr Leu Lys

85 90 95

aag gct gtc gat ggt ttg caa cca gaa gtc acc aga ttc ttg gaa caa 336

Lys Ala Val Asp Gly Leu Gln Pro Glu Val Thr Arg Phe Leu Glu Gln

100 105 110

cac tct cca gac tgg atc atc tac gac ttc act cac tac tgg ttg cca 384

His Ser Pro Asp Trp Ile Ile Tyr Asp Phe Thr His Tyr Trp Leu Pro

115 120 125

tcc att gct gct tct ttg ggt atc tcc aga gct tac ttc tgt gtc atc 432

Ser Ile Ala Ala Ser Leu Gly Ile Ser Arg Ala Tyr Phe Cys Val Ile

130 135 140

act cca tgg acc att gct tac ttg gct cca tct tct gat gct atg att 480

Thr Pro Trp Thr Ile Ala Tyr Leu Ala Pro Ser Ser Asp Ala Met Ile

145 150 155 160

aac gat tct gat ggt aga acc acc gtc gaa gac ttg acc act cca cca 528

Asn Asp Ser Asp Gly Arg Thr Thr Val Glu Asp Leu Thr Thr Pro Pro

165 170 175

aaa tgg ttc cca ttc cca acc aag gtc tgt tgg aga aag cac gac ttg 576

Lys Trp Phe Pro Phe Pro Thr Lys Val Cys Trp Arg Lys His Asp Leu

180 185 190

gcc aga atg gaa cca tac gaa gct cca ggt atc tcc gat ggt tac aga 624

Ala Arg Met Glu Pro Tyr Glu Ala Pro Gly Ile Ser Asp Gly Tyr Arg

195 200 205

atg ggt atg gtt ttc aag ggt tcc gac tgt cta tta ttc aaa tgt tac 672

Met Gly Met Val Phe Lys Gly Ser Asp Cys Leu Leu Phe Lys Cys Tyr

210 215 220

cat gaa ttt ggt act caa tgg tta cca ttg ttg gaa act ttg cac caa 720

His Glu Phe Gly Thr Gln Trp Leu Pro Leu Leu Glu Thr Leu His Gln

225 230 235 240

gtc cca gtt gtt cca gtt ggt ttg ttg cct cca gaa atc cca ggt gat 768

Val Pro Val Val Pro Val Gly Leu Leu Pro Pro Glu Ile Pro Gly Asp

245 250 255

gaa aag gac gaa acc tgg gtt tcc atc aag aaa tgg ttg gac ggt aag 816

Glu Lys Asp Glu Thr Trp Val Ser Ile Lys Lys Trp Leu Asp Gly Lys

260 265 270

caa aag ggt tct gtt gtc tac gtt gct ttg ggt tct gaa gct ttg gtt 864

Gln Lys Gly Ser Val Val Tyr Val Ala Leu Gly Ser Glu Ala Leu Val

275 280 285

tct caa act gaa gtt gtc gaa ttg gct tta ggt ttg gaa ttg tcc ggt 912

Ser Gln Thr Glu Val Val Glu Leu Ala Leu Gly Leu Glu Leu Ser Gly

290 295 300

ttg cca ttc gtc tgg gct tac aga aag cca aag ggt cca gct aag tct 960

Leu Pro Phe Val Trp Ala Tyr Arg Lys Pro Lys Gly Pro Ala Lys Ser

305 310 315 320

gac tct gtt gaa ttg cca gat ggt ttc gtt gaa aga acc aga gac aga 1008

Asp Ser Val Glu Leu Pro Asp Gly Phe Val Glu Arg Thr Arg Asp Arg

325 330 335

ggt ttg gtc tgg act tct tgg gct cca caa ttg aga att ttg tcc cac 1056

Gly Leu Val Trp Thr Ser Trp Ala Pro Gln Leu Arg Ile Leu Ser His

340 345 350

gaa tct gtc tgt ggt ttc ttg act cac tgt ggt tct ggt tcc att gtc 1104

Glu Ser Val Cys Gly Phe Leu Thr His Cys Gly Ser Gly Ser Ile Val

355 360 365

gaa ggt ttg atg ttc ggt cat cca ttg atc atg ttg cca tta ttc ggt 1152

Glu Gly Leu Met Phe Gly His Pro Leu Ile Met Leu Pro Leu Phe Gly

370 375 380

gac caa cca ttg aac gct aga tta ttg gaa gac aaa caa gtc ggt att 1200

Asp Gln Pro Leu Asn Ala Arg Leu Leu Glu Asp Lys Gln Val Gly Ile

385 390 395 400

gaa att cca aga aac gaa gaa gat ggt tgt ttg acc aag gaa tcc gtt 1248

Glu Ile Pro Arg Asn Glu Glu Asp Gly Cys Leu Thr Lys Glu Ser Val

405 410 415

gct cgt tct tta cgt tct gtc gtt gtc gaa aac gaa ggt gaa atc tac 1296

Ala Arg Ser Leu Arg Ser Val Val Val Glu Asn Glu Gly Glu Ile Tyr

420 425 430

aag gcc aat gcc aga gaa ttg tcc aag atc tac aac gac acc aag gtt 1344

Lys Ala Asn Ala Arg Glu Leu Ser Lys Ile Tyr Asn Asp Thr Lys Val

435 440 445

gaa aag gaa tac gtt tct caa ttt gtc gac tac ttg gaa aag aac gcc 1392

Glu Lys Glu Tyr Val Ser Gln Phe Val Asp Tyr Leu Glu Lys Asn Ala

450 455 460

aga gct gtt gcc atc gac cac gaa tcc 1419

Arg Ala Val Ala Ile Asp His Glu Ser

465 470

<210> 110

<211> 473

<212> PRT

<213> Stevia rebaudiana

<400> 110

Met Ala Thr Ser Asp Ser Ile Val Asp Asp Arg Lys Gln Leu His Val

1 5 10 15

Ala Thr Phe Pro Trp Leu Ala Phe Gly His Ile Leu Pro Phe Leu Gln

20 25 30

Leu Ser Lys Leu Ile Ala Glu Lys Gly His Lys Val Ser Phe Leu Ser

35 40 45

Thr Thr Arg Asn Ile Gln Arg Leu Ser Ser His Ile Ser Pro Leu Ile

50 55 60

Asn Val Val Gln Leu Thr Leu Pro Arg Val Gln Glu Leu Pro Glu Asp

65 70 75 80

Ala Glu Ala Thr Thr Asp Val His Pro Glu Asp Ile Gln Tyr Leu Lys

85 90 95

Lys Ala Val Asp Gly Leu Gln Pro Glu Val Thr Arg Phe Leu Glu Gln

100 105 110

His Ser Pro Asp Trp Ile Ile Tyr Asp Phe Thr His Tyr Trp Leu Pro

115 120 125

Ser Ile Ala Ala Ser Leu Gly Ile Ser Arg Ala Tyr Phe Cys Val Ile

130 135 140

Thr Pro Trp Thr Ile Ala Tyr Leu Ala Pro Ser Ser Asp Ala Met Ile

145 150 155 160

Asn Asp Ser Asp Gly Arg Thr Thr Val Glu Asp Leu Thr Thr Pro Pro

165 170 175

Lys Trp Phe Pro Phe Pro Thr Lys Val Cys Trp Arg Lys His Asp Leu

180 185 190

Ala Arg Met Glu Pro Tyr Glu Ala Pro Gly Ile Ser Asp Gly Tyr Arg

195 200 205

Met Gly Met Val Phe Lys Gly Ser Asp Cys Leu Leu Phe Lys Cys Tyr

210 215 220

His Glu Phe Gly Thr Gln Trp Leu Pro Leu Leu Glu Thr Leu His Gln

225 230 235 240

Val Pro Val Val Pro Val Gly Leu Leu Pro Pro Glu Ile Pro Gly Asp

245 250 255

Glu Lys Asp Glu Thr Trp Val Ser Ile Lys Lys Trp Leu Asp Gly Lys

260 265 270

Gln Lys Gly Ser Val Val Tyr Val Ala Leu Gly Ser Glu Ala Leu Val

275 280 285

Ser Gln Thr Glu Val Val Glu Leu Ala Leu Gly Leu Glu Leu Ser Gly

290 295 300

Leu Pro Phe Val Trp Ala Tyr Arg Lys Pro Lys Gly Pro Ala Lys Ser

305 310 315 320

Asp Ser Val Glu Leu Pro Asp Gly Phe Val Glu Arg Thr Arg Asp Arg

325 330 335

Gly Leu Val Trp Thr Ser Trp Ala Pro Gln Leu Arg Ile Leu Ser His

340 345 350

Glu Ser Val Cys Gly Phe Leu Thr His Cys Gly Ser Gly Ser Ile Val

355 360 365

Glu Gly Leu Met Phe Gly His Pro Leu Ile Met Leu Pro Leu Phe Gly

370 375 380

Asp Gln Pro Leu Asn Ala Arg Leu Leu Glu Asp Lys Gln Val Gly Ile

385 390 395 400

Glu Ile Pro Arg Asn Glu Glu Asp Gly Cys Leu Thr Lys Glu Ser Val

405 410 415

Ala Arg Ser Leu Arg Ser Val Val Val Glu Asn Glu Gly Glu Ile Tyr

420 425 430

Lys Ala Asn Ala Arg Glu Leu Ser Lys Ile Tyr Asn Asp Thr Lys Val

435 440 445

Glu Lys Glu Tyr Val Ser Gln Phe Val Asp Tyr Leu Glu Lys Asn Ala

450 455 460

Arg Ala Val Ala Ile Asp His Glu Ser

465 470

<210> 111

<211> 1356

<212> DNA

<213> Populus trichocarpa

<220>

<221> CDS

<222> (1)..(1356)

<400> 111

atg gac gaa cct cac gaa ttg cac att gct atg ttc cca tgg ttg gcc 48

Met Asp Glu Pro His Glu Leu His Ile Ala Met Phe Pro Trp Leu Ala

1 5 10 15

ttt ggt cac atc att cca ttc ttg gaa tta gcc aag ttg att gct caa 96

Phe Gly His Ile Ile Pro Phe Leu Glu Leu Ala Lys Leu Ile Ala Gln

20 25 30

aga ggt cac aag atc tct ttc atc tcc act cca aga aac atc caa aga 144

Arg Gly His Lys Ile Ser Phe Ile Ser Thr Pro Arg Asn Ile Gln Arg

35 40 45

tta cca acc atc cca cca aac ttg acc cca aga atc aac tta gtc tct 192

Leu Pro Thr Ile Pro Pro Asn Leu Thr Pro Arg Ile Asn Leu Val Ser

50 55 60

ttg gct ttg cca cac gtt gaa aac ttg cca aac aac gct gaa gcc act 240

Leu Ala Leu Pro His Val Glu Asn Leu Pro Asn Asn Ala Glu Ala Thr

65 70 75 80

gct gac ttg cca ttc gac aag atc cca tac ttg aag att gct tac gac 288

Ala Asp Leu Pro Phe Asp Lys Ile Pro Tyr Leu Lys Ile Ala Tyr Asp

85 90 95

aga ttg caa gac tct ttg ttc cat ttc ttg cac tct tct tct cca gac 336

Arg Leu Gln Asp Ser Leu Phe His Phe Leu His Ser Ser Ser Pro Asp

100 105 110

tgg atc atc ttc gat ttt gcc tcc tac tgg tta cca gaa att gct acc 384

Trp Ile Ile Phe Asp Phe Ala Ser Tyr Trp Leu Pro Glu Ile Ala Thr

115 120 125

aaa ttg ggt atc tcc ggt gtt ttg ttc tcc atc ttc ggt gct tgg act 432

Lys Leu Gly Ile Ser Gly Val Leu Phe Ser Ile Phe Gly Ala Trp Thr

130 135 140

tta tct ttc gct ggt cca tct tac tct gcc att ttg aac ggt gat gac 480

Leu Ser Phe Ala Gly Pro Ser Tyr Ser Ala Ile Leu Asn Gly Asp Asp

145 150 155 160

cca aga acc gaa cca caa cat ttc acc gtt cca cca aaa tgg gtc act 528

Pro Arg Thr Glu Pro Gln His Phe Thr Val Pro Pro Lys Trp Val Thr

165 170 175

ttc cca tcc aag gtt gct ttc cgt atc cac gaa gct aag aga ttc ttg 576

Phe Pro Ser Lys Val Ala Phe Arg Ile His Glu Ala Lys Arg Phe Leu

180 185 190

gtt caa atc gaa gct aac tct tct ggt gtc act gac atc ttc aga tgg 624

Val Gln Ile Glu Ala Asn Ser Ser Gly Val Thr Asp Ile Phe Arg Trp

195 200 205

ggt tcc gtt ttg gct ggt tgt gat gtc att gcc gtc aga tct tgt ttg 672

Gly Ser Val Leu Ala Gly Cys Asp Val Ile Ala Val Arg Ser Cys Leu

210 215 220

gaa ttg gaa gct gac ttc ttg aga ttg gtc gaa gat ttg cac tgt aag 720

Glu Leu Glu Ala Asp Phe Leu Arg Leu Val Glu Asp Leu His Cys Lys

225 230 235 240

cca gtt atc cca gtc ggt cta tta cca cct cca gct caa tgt tct gaa 768

Pro Val Ile Pro Val Gly Leu Leu Pro Pro Pro Ala Gln Cys Ser Glu

245 250 255

ggt ggt tcc aga gaa ggt ggt gtc gac gaa aaa tgg gtt acc att tct 816

Gly Gly Ser Arg Glu Gly Gly Val Asp Glu Lys Trp Val Thr Ile Ser

260 265 270

gaa tgg ttg gac aag caa act caa ggt tcc gtt gtc tac att gct ttc 864

Glu Trp Leu Asp Lys Gln Thr Gln Gly Ser Val Val Tyr Ile Ala Phe

275 280 285

ggt tct gaa ttg acc atc aac caa aat gaa atc act gaa ttg gct ttg 912

Gly Ser Glu Leu Thr Ile Asn Gln Asn Glu Ile Thr Glu Leu Ala Leu

290 295 300

ggt tta gaa ttg tct ggt ttg cca ttc ttc tgg gct ttc aga aac aga 960

Gly Leu Glu Leu Ser Gly Leu Pro Phe Phe Trp Ala Phe Arg Asn Arg

305 310 315 320

gat gac tcc gtt aga ttg cca gat ggt ttc gat gaa aga gtc aag ggt 1008

Asp Asp Ser Val Arg Leu Pro Asp Gly Phe Asp Glu Arg Val Lys Gly

325 330 335

cgt ggt gtt gtc tgg act tcc tgg gct cct caa ttg aga atc atg gct 1056

Arg Gly Val Val Trp Thr Ser Trp Ala Pro Gln Leu Arg Ile Met Ala

340 345 350

cac gaa tcc gtt ggt ggt ttc ttg act cac tgt ggt tac tcc tct gtt 1104

His Glu Ser Val Gly Gly Phe Leu Thr His Cys Gly Tyr Ser Ser Val

355 360 365

atc gaa gct ttg tcc ttc ggt ttg gct tta atc atg ttg cca ttt gcc 1152

Ile Glu Ala Leu Ser Phe Gly Leu Ala Leu Ile Met Leu Pro Phe Ala

370 375 380

att gac caa ggt ttg att gcc cgt gtt ttc gaa ggt aag aag gtc ggt 1200

Ile Asp Gln Gly Leu Ile Ala Arg Val Phe Glu Gly Lys Lys Val Gly

385 390 395 400

att gaa gtc cca aga gat gaa caa gat ggt tcc ttc acc aga aac tct 1248

Ile Glu Val Pro Arg Asp Glu Gln Asp Gly Ser Phe Thr Arg Asn Ser

405 410 415

gtt gct gaa tct cta aga ttg gtc att gtt gac aag gaa ggt tct gct 1296

Val Ala Glu Ser Leu Arg Leu Val Ile Val Asp Lys Glu Gly Ser Ala

420 425 430

tac aga gaa aac gct aag caa caa atg gtt act ttg ttt ggt ttg acc 1344

Tyr Arg Glu Asn Ala Lys Gln Gln Met Val Thr Leu Phe Gly Leu Thr

435 440 445

tac acc atg acg 1356

Tyr Thr Met Thr

450

<210> 112

<211> 452

<212> PRT

<213> Populus trichocarpa

<400> 112

Met Asp Glu Pro His Glu Leu His Ile Ala Met Phe Pro Trp Leu Ala

1 5 10 15

Phe Gly His Ile Ile Pro Phe Leu Glu Leu Ala Lys Leu Ile Ala Gln

20 25 30

Arg Gly His Lys Ile Ser Phe Ile Ser Thr Pro Arg Asn Ile Gln Arg

35 40 45

Leu Pro Thr Ile Pro Pro Asn Leu Thr Pro Arg Ile Asn Leu Val Ser

50 55 60

Leu Ala Leu Pro His Val Glu Asn Leu Pro Asn Asn Ala Glu Ala Thr

65 70 75 80

Ala Asp Leu Pro Phe Asp Lys Ile Pro Tyr Leu Lys Ile Ala Tyr Asp

85 90 95

Arg Leu Gln Asp Ser Leu Phe His Phe Leu His Ser Ser Ser Pro Asp

100 105 110

Trp Ile Ile Phe Asp Phe Ala Ser Tyr Trp Leu Pro Glu Ile Ala Thr

115 120 125

Lys Leu Gly Ile Ser Gly Val Leu Phe Ser Ile Phe Gly Ala Trp Thr

130 135 140

Leu Ser Phe Ala Gly Pro Ser Tyr Ser Ala Ile Leu Asn Gly Asp Asp

145 150 155 160

Pro Arg Thr Glu Pro Gln His Phe Thr Val Pro Pro Lys Trp Val Thr

165 170 175

Phe Pro Ser Lys Val Ala Phe Arg Ile His Glu Ala Lys Arg Phe Leu

180 185 190

Val Gln Ile Glu Ala Asn Ser Ser Gly Val Thr Asp Ile Phe Arg Trp

195 200 205

Gly Ser Val Leu Ala Gly Cys Asp Val Ile Ala Val Arg Ser Cys Leu

210 215 220

Glu Leu Glu Ala Asp Phe Leu Arg Leu Val Glu Asp Leu His Cys Lys

225 230 235 240

Pro Val Ile Pro Val Gly Leu Leu Pro Pro Pro Ala Gln Cys Ser Glu

245 250 255

Gly Gly Ser Arg Glu Gly Gly Val Asp Glu Lys Trp Val Thr Ile Ser

260 265 270

Glu Trp Leu Asp Lys Gln Thr Gln Gly Ser Val Val Tyr Ile Ala Phe

275 280 285

Gly Ser Glu Leu Thr Ile Asn Gln Asn Glu Ile Thr Glu Leu Ala Leu

290 295 300

Gly Leu Glu Leu Ser Gly Leu Pro Phe Phe Trp Ala Phe Arg Asn Arg

305 310 315 320

Asp Asp Ser Val Arg Leu Pro Asp Gly Phe Asp Glu Arg Val Lys Gly

325 330 335

Arg Gly Val Val Trp Thr Ser Trp Ala Pro Gln Leu Arg Ile Met Ala

340 345 350

His Glu Ser Val Gly Gly Phe Leu Thr His Cys Gly Tyr Ser Ser Val

355 360 365

Ile Glu Ala Leu Ser Phe Gly Leu Ala Leu Ile Met Leu Pro Phe Ala

370 375 380

Ile Asp Gln Gly Leu Ile Ala Arg Val Phe Glu Gly Lys Lys Val Gly

385 390 395 400

Ile Glu Val Pro Arg Asp Glu Gln Asp Gly Ser Phe Thr Arg Asn Ser

405 410 415

Val Ala Glu Ser Leu Arg Leu Val Ile Val Asp Lys Glu Gly Ser Ala

420 425 430

Tyr Arg Glu Asn Ala Lys Gln Gln Met Val Thr Leu Phe Gly Leu Thr

435 440 445

Tyr Thr Met Thr

450

<210> 113

<211> 1362

<212> DNA

<213> Stevia rebaudiana

<220>

<221> CDS

<222> (1)..(1362)

<400> 113

atg tcc ttg aaa ggt aac gac aag gaa tta cat ttg gtc atg ttc cca 48

Met Ser Leu Lys Gly Asn Asp Lys Glu Leu His Leu Val Met Phe Pro

1 5 10 15

ttc ttt gct ttc ggt cac atc act cca ttt gtc caa ttg tcc aac aag 96

Phe Phe Ala Phe Gly His Ile Thr Pro Phe Val Gln Leu Ser Asn Lys

20 25 30

atc tcc tct ttg tac cca ggt gtc aag atc act ttc ttg gct gct tct 144

Ile Ser Ser Leu Tyr Pro Gly Val Lys Ile Thr Phe Leu Ala Ala Ser

35 40 45

gct tct gtt tcc aga atc gaa acc atg ttg aac cca tct acc aac acc 192

Ala Ser Val Ser Arg Ile Glu Thr Met Leu Asn Pro Ser Thr Asn Thr

50 55 60

aag gtc att cca ttg act ttg cca aga gtc gat ggt cta cca gaa ggt 240

Lys Val Ile Pro Leu Thr Leu Pro Arg Val Asp Gly Leu Pro Glu Gly

65 70 75 80

gtt gaa aac act gct gac gct tct cca gct acc att ggt cta ttg gtt 288

Val Glu Asn Thr Ala Asp Ala Ser Pro Ala Thr Ile Gly Leu Leu Val

85 90 95

gtt gcc att gat ttg atg caa cct caa atc aag act tta ttg gct aac 336

Val Ala Ile Asp Leu Met Gln Pro Gln Ile Lys Thr Leu Leu Ala Asn

100 105 110

ttg aag cca gac ttc gtt atc ttc gac ttc gtc cac tgg tgg tta cca 384

Leu Lys Pro Asp Phe Val Ile Phe Asp Phe Val His Trp Trp Leu Pro

115 120 125

gaa att gct tct gaa ttg ggt atc aag acc atc tac ttc tcc gtt tac 432

Glu Ile Ala Ser Glu Leu Gly Ile Lys Thr Ile Tyr Phe Ser Val Tyr

130 135 140

atg gcc aac att gtc atg cca tcc act tcc aag ttg acc ggt aac aag 480

Met Ala Asn Ile Val Met Pro Ser Thr Ser Lys Leu Thr Gly Asn Lys

145 150 155 160

cca tct acc gtc gaa gat atc aag gct ttg caa caa tct tac ggt att 528

Pro Ser Thr Val Glu Asp Ile Lys Ala Leu Gln Gln Ser Tyr Gly Ile

165 170 175

cca gtt aag act ttc gaa gcc atc tct ttg atg aac gtt ttc aag tct 576

Pro Val Lys Thr Phe Glu Ala Ile Ser Leu Met Asn Val Phe Lys Ser

180 185 190

ttc cac gac tgg atg gac aaa tgt atc aac ggt tgt aac ttg atg ttg 624

Phe His Asp Trp Met Asp Lys Cys Ile Asn Gly Cys Asn Leu Met Leu

195 200 205

atc aag tct tgt cgt gaa atg gaa ggt tcc aga att gac gat gtc acc 672

Ile Lys Ser Cys Arg Glu Met Glu Gly Ser Arg Ile Asp Asp Val Thr

210 215 220

aag caa tct acc aga cca gtt ttc ttg att ggt cca gtt gtt cca gaa 720

Lys Gln Ser Thr Arg Pro Val Phe Leu Ile Gly Pro Val Val Pro Glu

225 230 235 240

cct cac tct ggt gaa ttg gac gaa acc tgg gct aac tgg ttg aac aga 768

Pro His Ser Gly Glu Leu Asp Glu Thr Trp Ala Asn Trp Leu Asn Arg

245 250 255

ttc cca gct aag tct gtc atc tac tgt tcc ttt ggt tct gaa act ttc 816

Phe Pro Ala Lys Ser Val Ile Tyr Cys Ser Phe Gly Ser Glu Thr Phe

260 265 270

ttg acc gat gac caa atc aga gaa ttg gcc ttg ggt tta gaa ttg act 864

Leu Thr Asp Asp Gln Ile Arg Glu Leu Ala Leu Gly Leu Glu Leu Thr

275 280 285

ggt ttg cca ttc ttc ttg gtc ttg aac ttc cca gcc aat gtc gac aaa 912

Gly Leu Pro Phe Phe Leu Val Leu Asn Phe Pro Ala Asn Val Asp Lys

290 295 300

tct gct gaa ttg aag aga act tta cca gat ggt ttc ttg gaa aga gtt 960

Ser Ala Glu Leu Lys Arg Thr Leu Pro Asp Gly Phe Leu Glu Arg Val

305 310 315 320

aag gac aag ggt att gtc cac tct ggt tgg gtt caa caa aga cac atc 1008

Lys Asp Lys Gly Ile Val His Ser Gly Trp Val Gln Gln Arg His Ile

325 330 335

ttg gct cat gac tct gtt ggt tgt tac gtt ttc cac gct ggt tac ggt 1056

Leu Ala His Asp Ser Val Gly Cys Tyr Val Phe His Ala Gly Tyr Gly

340 345 350

tcc gtt atc gaa ggt ttg gtc aat gac tgt caa tta gtc atg ttg cca 1104

Ser Val Ile Glu Gly Leu Val Asn Asp Cys Gln Leu Val Met Leu Pro

355 360 365

atg aag gtt gac caa ttc acc aac tcc aag gtt att gct ttg gaa ttg 1152

Met Lys Val Asp Gln Phe Thr Asn Ser Lys Val Ile Ala Leu Glu Leu

370 375 380

aag gct ggt gtt gaa gtt aac aga aga gat gaa gat ggt tac ttc ggt 1200

Lys Ala Gly Val Glu Val Asn Arg Arg Asp Glu Asp Gly Tyr Phe Gly

385 390 395 400

aag gac gat gtc ttc gaa gct gtc gaa tct gtc atg atg gac act gaa 1248

Lys Asp Asp Val Phe Glu Ala Val Glu Ser Val Met Met Asp Thr Glu

405 410 415

aac gaa cca gcc aag tcc atc aga gaa aac cac cgt aaa ttg aag gaa 1296

Asn Glu Pro Ala Lys Ser Ile Arg Glu Asn His Arg Lys Leu Lys Glu

420 425 430

ttt ttg caa aac gat gaa atc caa aag aaa tac att gct gat ttc gtt 1344

Phe Leu Gln Asn Asp Glu Ile Gln Lys Lys Tyr Ile Ala Asp Phe Val

435 440 445

gaa aac ttg aaa gcg tta 1362

Glu Asn Leu Lys Ala Leu

450

<210> 114

<211> 454

<212> PRT

<213> Stevia rebaudiana

<400> 114

Met Ser Leu Lys Gly Asn Asp Lys Glu Leu His Leu Val Met Phe Pro

1 5 10 15

Phe Phe Ala Phe Gly His Ile Thr Pro Phe Val Gln Leu Ser Asn Lys

20 25 30

Ile Ser Ser Leu Tyr Pro Gly Val Lys Ile Thr Phe Leu Ala Ala Ser

35 40 45

Ala Ser Val Ser Arg Ile Glu Thr Met Leu Asn Pro Ser Thr Asn Thr

50 55 60

Lys Val Ile Pro Leu Thr Leu Pro Arg Val Asp Gly Leu Pro Glu Gly

65 70 75 80

Val Glu Asn Thr Ala Asp Ala Ser Pro Ala Thr Ile Gly Leu Leu Val

85 90 95

Val Ala Ile Asp Leu Met Gln Pro Gln Ile Lys Thr Leu Leu Ala Asn

100 105 110

Leu Lys Pro Asp Phe Val Ile Phe Asp Phe Val His Trp Trp Leu Pro

115 120 125

Glu Ile Ala Ser Glu Leu Gly Ile Lys Thr Ile Tyr Phe Ser Val Tyr

130 135 140

Met Ala Asn Ile Val Met Pro Ser Thr Ser Lys Leu Thr Gly Asn Lys

145 150 155 160

Pro Ser Thr Val Glu Asp Ile Lys Ala Leu Gln Gln Ser Tyr Gly Ile

165 170 175

Pro Val Lys Thr Phe Glu Ala Ile Ser Leu Met Asn Val Phe Lys Ser

180 185 190

Phe His Asp Trp Met Asp Lys Cys Ile Asn Gly Cys Asn Leu Met Leu

195 200 205

Ile Lys Ser Cys Arg Glu Met Glu Gly Ser Arg Ile Asp Asp Val Thr

210 215 220

Lys Gln Ser Thr Arg Pro Val Phe Leu Ile Gly Pro Val Val Pro Glu

225 230 235 240

Pro His Ser Gly Glu Leu Asp Glu Thr Trp Ala Asn Trp Leu Asn Arg

245 250 255

Phe Pro Ala Lys Ser Val Ile Tyr Cys Ser Phe Gly Ser Glu Thr Phe

260 265 270

Leu Thr Asp Asp Gln Ile Arg Glu Leu Ala Leu Gly Leu Glu Leu Thr

275 280 285

Gly Leu Pro Phe Phe Leu Val Leu Asn Phe Pro Ala Asn Val Asp Lys

290 295 300

Ser Ala Glu Leu Lys Arg Thr Leu Pro Asp Gly Phe Leu Glu Arg Val

305 310 315 320

Lys Asp Lys Gly Ile Val His Ser Gly Trp Val Gln Gln Arg His Ile

325 330 335

Leu Ala His Asp Ser Val Gly Cys Tyr Val Phe His Ala Gly Tyr Gly

340 345 350

Ser Val Ile Glu Gly Leu Val Asn Asp Cys Gln Leu Val Met Leu Pro

355 360 365

Met Lys Val Asp Gln Phe Thr Asn Ser Lys Val Ile Ala Leu Glu Leu

370 375 380

Lys Ala Gly Val Glu Val Asn Arg Arg Asp Glu Asp Gly Tyr Phe Gly

385 390 395 400

Lys Asp Asp Val Phe Glu Ala Val Glu Ser Val Met Met Asp Thr Glu

405 410 415

Asn Glu Pro Ala Lys Ser Ile Arg Glu Asn His Arg Lys Leu Lys Glu

420 425 430

Phe Leu Gln Asn Asp Glu Ile Gln Lys Lys Tyr Ile Ala Asp Phe Val

435 440 445

Glu Asn Leu Lys Ala Leu

450

<210> 115

<211> 1362

<212> DNA

<213> Stevia rebaudiana

<220>

<221> CDS

<222> (1)..(1362)

<400> 115

atg tcc ttg aaa ggt aac gac aag gaa tta cat ttg gtc atg ttc cca 48

Met Ser Leu Lys Gly Asn Asp Lys Glu Leu His Leu Val Met Phe Pro

1 5 10 15

ttc ttt gct ttc ggt cac atc act cca ttt gtc caa ttg tcc aac aag 96

Phe Phe Ala Phe Gly His Ile Thr Pro Phe Val Gln Leu Ser Asn Lys

20 25 30

atc tcc tct ttg tac cca ggt gtc aag atc act ttc ttg gct gct tct 144

Ile Ser Ser Leu Tyr Pro Gly Val Lys Ile Thr Phe Leu Ala Ala Ser

35 40 45

gct tct gtt tcc aga atc gaa acc atg ttg aac cca tct acc aac acc 192

Ala Ser Val Ser Arg Ile Glu Thr Met Leu Asn Pro Ser Thr Asn Thr

50 55 60

aag gtc att cca ttg act ttg cca aga gtc gat ggt cta cca gaa ggt 240

Lys Val Ile Pro Leu Thr Leu Pro Arg Val Asp Gly Leu Pro Glu Gly

65 70 75 80

gtt gaa aac act gct gac gct tct cca gct acc att ggt cta ttg gtt 288

Val Glu Asn Thr Ala Asp Ala Ser Pro Ala Thr Ile Gly Leu Leu Val

85 90 95

gtt gcc att gat ttg atg caa cct caa atc aag act tta ttg gct aac 336

Val Ala Ile Asp Leu Met Gln Pro Gln Ile Lys Thr Leu Leu Ala Asn

100 105 110

ttg aag cca gac ttc gtt atc ttc gac ttc gtc cac tgg tgg tta cca 384

Leu Lys Pro Asp Phe Val Ile Phe Asp Phe Val His Trp Trp Leu Pro

115 120 125

gaa att gct tct gaa ttg ggt atc aag acc atc tac ttc tcc gtt tac 432

Glu Ile Ala Ser Glu Leu Gly Ile Lys Thr Ile Tyr Phe Ser Val Tyr

130 135 140

atg gcc aac att gtc atg cca tcc act tcc aag ttg acc ggt aac aag 480

Met Ala Asn Ile Val Met Pro Ser Thr Ser Lys Leu Thr Gly Asn Lys

145 150 155 160

cca tct acc gtc gaa gat atc aag gct ttg caa caa tct gac ggt att 528

Pro Ser Thr Val Glu Asp Ile Lys Ala Leu Gln Gln Ser Asp Gly Ile

165 170 175

cca gtt aag act ttc gaa gcc atc tct ttg atg aac gtt ttc aag tct 576

Pro Val Lys Thr Phe Glu Ala Ile Ser Leu Met Asn Val Phe Lys Ser

180 185 190

ttc cac gac tgg atg gac aaa tgt atc aac ggt tgt aac ttg atg ttg 624

Phe His Asp Trp Met Asp Lys Cys Ile Asn Gly Cys Asn Leu Met Leu

195 200 205

atc aag tct tgt cgt gaa atg gaa ggt tcc aga att gac gat gtc acc 672

Ile Lys Ser Cys Arg Glu Met Glu Gly Ser Arg Ile Asp Asp Val Thr

210 215 220

aag caa tct acc aga cca gtt ttc ttg att ggt cca gtt gtt cca gaa 720

Lys Gln Ser Thr Arg Pro Val Phe Leu Ile Gly Pro Val Val Pro Glu

225 230 235 240

cct cac tct ggt gaa ttg gac gaa acc tgg gct aac tgg ttg aac aga 768

Pro His Ser Gly Glu Leu Asp Glu Thr Trp Ala Asn Trp Leu Asn Arg

245 250 255

ttc cca gct aag tct gtc atc tac tgt tcc ttt ggt tct gaa act ttc 816

Phe Pro Ala Lys Ser Val Ile Tyr Cys Ser Phe Gly Ser Glu Thr Phe

260 265 270

ttg acc gat gac caa atc aga gaa ttg gcc ttg ggt tta gaa ttg act 864

Leu Thr Asp Asp Gln Ile Arg Glu Leu Ala Leu Gly Leu Glu Leu Thr

275 280 285

ggt ttg cca ttc ttc ttg gtc ttg aac ttc cca gcc aat gtc gac aaa 912

Gly Leu Pro Phe Phe Leu Val Leu Asn Phe Pro Ala Asn Val Asp Lys

290 295 300

tct gct gaa ttg aag aga act tta cca gat ggt ttc ttg gaa aga gtt 960

Ser Ala Glu Leu Lys Arg Thr Leu Pro Asp Gly Phe Leu Glu Arg Val

305 310 315 320

aag gac aag ggt att gtc cac tct ggt tgg gtt caa caa aga cac atc 1008

Lys Asp Lys Gly Ile Val His Ser Gly Trp Val Gln Gln Arg His Ile

325 330 335

ttg gct cat gac tct gtt ggt tgt tac gtt ttc cac gct ggt tac ggt 1056

Leu Ala His Asp Ser Val Gly Cys Tyr Val Phe His Ala Gly Tyr Gly

340 345 350

tcc gtt atc gaa ggt ttg gtc aat gac tgt caa tta gtc atg ttg cca 1104

Ser Val Ile Glu Gly Leu Val Asn Asp Cys Gln Leu Val Met Leu Pro

355 360 365

atg aag gtt gac caa ttc acc aac tcc aag gtt att gct ttg gaa ttg 1152

Met Lys Val Asp Gln Phe Thr Asn Ser Lys Val Ile Ala Leu Glu Leu

370 375 380

aag gct ggt gtt gaa gtt aac aga aga gat gaa gat ggt tac ttc ggt 1200

Lys Ala Gly Val Glu Val Asn Arg Arg Asp Glu Asp Gly Tyr Phe Gly

385 390 395 400

aag gac gat gtc ttc gaa gct gtc gaa tct gtc atg atg gac act gaa 1248

Lys Asp Asp Val Phe Glu Ala Val Glu Ser Val Met Met Asp Thr Glu

405 410 415

aac gaa cca gcc aag tcc atc aga gaa aac cac cgt aaa ttg aag gaa 1296

Asn Glu Pro Ala Lys Ser Ile Arg Glu Asn His Arg Lys Leu Lys Glu

420 425 430

ttt ttg caa aac gat gaa atc caa aag aaa tac att gct gat ttc gtt 1344

Phe Leu Gln Asn Asp Glu Ile Gln Lys Lys Tyr Ile Ala Asp Phe Val

435 440 445

gaa aac ttg aaa gcg tta 1362

Glu Asn Leu Lys Ala Leu

450

<210> 116

<211> 454

<212> PRT

<213> Stevia rebaudiana

<400> 116

Met Ser Leu Lys Gly Asn Asp Lys Glu Leu His Leu Val Met Phe Pro

1 5 10 15

Phe Phe Ala Phe Gly His Ile Thr Pro Phe Val Gln Leu Ser Asn Lys

20 25 30

Ile Ser Ser Leu Tyr Pro Gly Val Lys Ile Thr Phe Leu Ala Ala Ser

35 40 45

Ala Ser Val Ser Arg Ile Glu Thr Met Leu Asn Pro Ser Thr Asn Thr

50 55 60

Lys Val Ile Pro Leu Thr Leu Pro Arg Val Asp Gly Leu Pro Glu Gly

65 70 75 80

Val Glu Asn Thr Ala Asp Ala Ser Pro Ala Thr Ile Gly Leu Leu Val

85 90 95

Val Ala Ile Asp Leu Met Gln Pro Gln Ile Lys Thr Leu Leu Ala Asn

100 105 110

Leu Lys Pro Asp Phe Val Ile Phe Asp Phe Val His Trp Trp Leu Pro

115 120 125

Glu Ile Ala Ser Glu Leu Gly Ile Lys Thr Ile Tyr Phe Ser Val Tyr

130 135 140

Met Ala Asn Ile Val Met Pro Ser Thr Ser Lys Leu Thr Gly Asn Lys

145 150 155 160

Pro Ser Thr Val Glu Asp Ile Lys Ala Leu Gln Gln Ser Asp Gly Ile

165 170 175

Pro Val Lys Thr Phe Glu Ala Ile Ser Leu Met Asn Val Phe Lys Ser

180 185 190

Phe His Asp Trp Met Asp Lys Cys Ile Asn Gly Cys Asn Leu Met Leu

195 200 205

Ile Lys Ser Cys Arg Glu Met Glu Gly Ser Arg Ile Asp Asp Val Thr

210 215 220

Lys Gln Ser Thr Arg Pro Val Phe Leu Ile Gly Pro Val Val Pro Glu

225 230 235 240

Pro His Ser Gly Glu Leu Asp Glu Thr Trp Ala Asn Trp Leu Asn Arg

245 250 255

Phe Pro Ala Lys Ser Val Ile Tyr Cys Ser Phe Gly Ser Glu Thr Phe

260 265 270

Leu Thr Asp Asp Gln Ile Arg Glu Leu Ala Leu Gly Leu Glu Leu Thr

275 280 285

Gly Leu Pro Phe Phe Leu Val Leu Asn Phe Pro Ala Asn Val Asp Lys

290 295 300

Ser Ala Glu Leu Lys Arg Thr Leu Pro Asp Gly Phe Leu Glu Arg Val

305 310 315 320

Lys Asp Lys Gly Ile Val His Ser Gly Trp Val Gln Gln Arg His Ile

325 330 335

Leu Ala His Asp Ser Val Gly Cys Tyr Val Phe His Ala Gly Tyr Gly

340 345 350

Ser Val Ile Glu Gly Leu Val Asn Asp Cys Gln Leu Val Met Leu Pro

355 360 365

Met Lys Val Asp Gln Phe Thr Asn Ser Lys Val Ile Ala Leu Glu Leu

370 375 380

Lys Ala Gly Val Glu Val Asn Arg Arg Asp Glu Asp Gly Tyr Phe Gly

385 390 395 400

Lys Asp Asp Val Phe Glu Ala Val Glu Ser Val Met Met Asp Thr Glu

405 410 415

Asn Glu Pro Ala Lys Ser Ile Arg Glu Asn His Arg Lys Leu Lys Glu

420 425 430

Phe Leu Gln Asn Asp Glu Ile Gln Lys Lys Tyr Ile Ala Asp Phe Val

435 440 445

Glu Asn Leu Lys Ala Leu

450

<210> 117

<211> 1449

<212> DNA

<213> Stevia rebaudiana

<220>

<221> CDS

<222> (1)..(1449)

<400> 117

atg gac caa atg gcc aag att gac gaa aag aag cct cac gtt gtt ttc 48

Met Asp Gln Met Ala Lys Ile Asp Glu Lys Lys Pro His Val Val Phe

1 5 10 15

att cca ttc cca gct caa tct cac atc aag tgt atg ttg aaa ttg gcc 96

Ile Pro Phe Pro Ala Gln Ser His Ile Lys Cys Met Leu Lys Leu Ala

20 25 30

aga att ttg cac caa aag ggt ttg tac atc act ttc atc aac act gac 144

Arg Ile Leu His Gln Lys Gly Leu Tyr Ile Thr Phe Ile Asn Thr Asp

35 40 45

acc aac cac gaa aga ttg gtt gcc tcc ggt ggt act caa tgg ttg gaa 192

Thr Asn His Glu Arg Leu Val Ala Ser Gly Gly Thr Gln Trp Leu Glu

50 55 60

aat gct cca ggt ttc tgg ttc aag acc gtc cca gat ggt ttc ggt tct 240

Asn Ala Pro Gly Phe Trp Phe Lys Thr Val Pro Asp Gly Phe Gly Ser

65 70 75 80

gcc aag gat gac ggt gtc aag cca act gat gct ttg aga gaa ttg atg 288

Ala Lys Asp Asp Gly Val Lys Pro Thr Asp Ala Leu Arg Glu Leu Met

85 90 95

gac tac ttg aag acc aac ttc ttc gat ttg ttc ttg gac ttg gtc ttg 336

Asp Tyr Leu Lys Thr Asn Phe Phe Asp Leu Phe Leu Asp Leu Val Leu

100 105 110

aag ttg gaa gtt cca gct acc tgt atc atc tgt gat ggt tgt atg acc 384

Lys Leu Glu Val Pro Ala Thr Cys Ile Ile Cys Asp Gly Cys Met Thr

115 120 125

ttt gct aac acc att aga gct gct gaa aag ttg aac att cca gtc atc 432

Phe Ala Asn Thr Ile Arg Ala Ala Glu Lys Leu Asn Ile Pro Val Ile

130 135 140

tta ttc tgg acc atg gct gct tgt ggt ttc atg gct ttc tac caa gct 480

Leu Phe Trp Thr Met Ala Ala Cys Gly Phe Met Ala Phe Tyr Gln Ala

145 150 155 160

aag gtc tta aag gaa aag gaa att gtt cca gtc aag gac gaa acc tac 528

Lys Val Leu Lys Glu Lys Glu Ile Val Pro Val Lys Asp Glu Thr Tyr

165 170 175

ttg acc aat ggt tac ttg gac atg gaa att gac tgg atc cca ggt atg 576

Leu Thr Asn Gly Tyr Leu Asp Met Glu Ile Asp Trp Ile Pro Gly Met

180 185 190

aag aga atc aga tta cgt gac ttg cct gaa ttc atc ttg gcc acc aag 624

Lys Arg Ile Arg Leu Arg Asp Leu Pro Glu Phe Ile Leu Ala Thr Lys

195 200 205

caa aac tac ttt gct ttc gaa ttc ttg ttt gaa act gct caa tta gct 672

Gln Asn Tyr Phe Ala Phe Glu Phe Leu Phe Glu Thr Ala Gln Leu Ala

210 215 220

gac aag gtt tct cac atg atc att cac act ttc gaa gaa ttg gaa gct 720

Asp Lys Val Ser His Met Ile Ile His Thr Phe Glu Glu Leu Glu Ala

225 230 235 240

tct tta gtc tct gaa atc aaa tct att ttc cca aac gtt tac act atc 768

Ser Leu Val Ser Glu Ile Lys Ser Ile Phe Pro Asn Val Tyr Thr Ile

245 250 255

ggt cca tta caa ttg ttg ttg aac aag atc act caa aag gaa acc aac 816

Gly Pro Leu Gln Leu Leu Leu Asn Lys Ile Thr Gln Lys Glu Thr Asn

260 265 270

aac gat tct tac tct tta tgg aag gaa gaa cca gaa tgt gtc gaa tgg 864

Asn Asp Ser Tyr Ser Leu Trp Lys Glu Glu Pro Glu Cys Val Glu Trp

275 280 285

ttg aac tcc aag gaa cca aac tcc gtt gtt tac gtc aac ttt ggt tct 912

Leu Asn Ser Lys Glu Pro Asn Ser Val Val Tyr Val Asn Phe Gly Ser

290 295 300

ttg gct gtc atg tct ttg caa gat ttg gtc gaa ttc ggt tgg ggt ttg 960

Leu Ala Val Met Ser Leu Gln Asp Leu Val Glu Phe Gly Trp Gly Leu

305 310 315 320

gtt aac tcc aac cat tac ttc tta tgg atc atc aga gct aac ttg att 1008

Val Asn Ser Asn His Tyr Phe Leu Trp Ile Ile Arg Ala Asn Leu Ile

325 330 335

gac ggt aag cca gct gtc atg cca caa gaa ttg aag gaa gcc atg aac 1056

Asp Gly Lys Pro Ala Val Met Pro Gln Glu Leu Lys Glu Ala Met Asn

340 345 350

gaa aaa ggt ttc gtt ggt tct tgg tgt tcc caa gaa gaa gtt ttg aac 1104

Glu Lys Gly Phe Val Gly Ser Trp Cys Ser Gln Glu Glu Val Leu Asn

355 360 365

cat cca gcc gtt ggt ggt ttc ttg act cac tgt ggc tgg ggt tcc atc 1152

His Pro Ala Val Gly Gly Phe Leu Thr His Cys Gly Trp Gly Ser Ile

370 375 380

att gaa tct cta tcc gct ggt gtt cca atg ttg ggt tgg cca tcc att 1200

Ile Glu Ser Leu Ser Ala Gly Val Pro Met Leu Gly Trp Pro Ser Ile

385 390 395 400

ggt gac caa aga gcc aac tgt cgt caa atg tgt aag gaa tgg gaa gtt 1248

Gly Asp Gln Arg Ala Asn Cys Arg Gln Met Cys Lys Glu Trp Glu Val

405 410 415

ggt atg gaa atc ggt aag aac gtt aag aga gat gaa gtc gaa aaa tta 1296

Gly Met Glu Ile Gly Lys Asn Val Lys Arg Asp Glu Val Glu Lys Leu

420 425 430

gtc aga atg ttg atg gaa ggt ttg gaa ggt gaa aga atg aga aag aag 1344

Val Arg Met Leu Met Glu Gly Leu Glu Gly Glu Arg Met Arg Lys Lys

435 440 445

gct ttg gaa tgg aag aaa tct gct act ttg gct act tgt tgt aac ggt 1392

Ala Leu Glu Trp Lys Lys Ser Ala Thr Leu Ala Thr Cys Cys Asn Gly

450 455 460

tcc tct tct ttg gat gtt gaa aaa ttg gct aac gaa atc aag aaa ttg 1440

Ser Ser Ser Leu Asp Val Glu Lys Leu Ala Asn Glu Ile Lys Lys Leu

465 470 475 480

tcc agg aac 1449

Ser Arg Asn

<210> 118

<211> 483

<212> PRT

<213> Stevia rebaudiana

<400> 118

Met Asp Gln Met Ala Lys Ile Asp Glu Lys Lys Pro His Val Val Phe

1 5 10 15

Ile Pro Phe Pro Ala Gln Ser His Ile Lys Cys Met Leu Lys Leu Ala

20 25 30

Arg Ile Leu His Gln Lys Gly Leu Tyr Ile Thr Phe Ile Asn Thr Asp

35 40 45

Thr Asn His Glu Arg Leu Val Ala Ser Gly Gly Thr Gln Trp Leu Glu

50 55 60

Asn Ala Pro Gly Phe Trp Phe Lys Thr Val Pro Asp Gly Phe Gly Ser

65 70 75 80

Ala Lys Asp Asp Gly Val Lys Pro Thr Asp Ala Leu Arg Glu Leu Met

85 90 95

Asp Tyr Leu Lys Thr Asn Phe Phe Asp Leu Phe Leu Asp Leu Val Leu

100 105 110

Lys Leu Glu Val Pro Ala Thr Cys Ile Ile Cys Asp Gly Cys Met Thr

115 120 125

Phe Ala Asn Thr Ile Arg Ala Ala Glu Lys Leu Asn Ile Pro Val Ile

130 135 140

Leu Phe Trp Thr Met Ala Ala Cys Gly Phe Met Ala Phe Tyr Gln Ala

145 150 155 160

Lys Val Leu Lys Glu Lys Glu Ile Val Pro Val Lys Asp Glu Thr Tyr

165 170 175

Leu Thr Asn Gly Tyr Leu Asp Met Glu Ile Asp Trp Ile Pro Gly Met

180 185 190

Lys Arg Ile Arg Leu Arg Asp Leu Pro Glu Phe Ile Leu Ala Thr Lys

195 200 205

Gln Asn Tyr Phe Ala Phe Glu Phe Leu Phe Glu Thr Ala Gln Leu Ala

210 215 220

Asp Lys Val Ser His Met Ile Ile His Thr Phe Glu Glu Leu Glu Ala

225 230 235 240

Ser Leu Val Ser Glu Ile Lys Ser Ile Phe Pro Asn Val Tyr Thr Ile

245 250 255

Gly Pro Leu Gln Leu Leu Leu Asn Lys Ile Thr Gln Lys Glu Thr Asn

260 265 270

Asn Asp Ser Tyr Ser Leu Trp Lys Glu Glu Pro Glu Cys Val Glu Trp

275 280 285

Leu Asn Ser Lys Glu Pro Asn Ser Val Val Tyr Val Asn Phe Gly Ser

290 295 300

Leu Ala Val Met Ser Leu Gln Asp Leu Val Glu Phe Gly Trp Gly Leu

305 310 315 320

Val Asn Ser Asn His Tyr Phe Leu Trp Ile Ile Arg Ala Asn Leu Ile

325 330 335

Asp Gly Lys Pro Ala Val Met Pro Gln Glu Leu Lys Glu Ala Met Asn

340 345 350

Glu Lys Gly Phe Val Gly Ser Trp Cys Ser Gln Glu Glu Val Leu Asn

355 360 365

His Pro Ala Val Gly Gly Phe Leu Thr His Cys Gly Trp Gly Ser Ile

370 375 380

Ile Glu Ser Leu Ser Ala Gly Val Pro Met Leu Gly Trp Pro Ser Ile

385 390 395 400

Gly Asp Gln Arg Ala Asn Cys Arg Gln Met Cys Lys Glu Trp Glu Val

405 410 415

Gly Met Glu Ile Gly Lys Asn Val Lys Arg Asp Glu Val Glu Lys Leu

420 425 430

Val Arg Met Leu Met Glu Gly Leu Glu Gly Glu Arg Met Arg Lys Lys

435 440 445

Ala Leu Glu Trp Lys Lys Ser Ala Thr Leu Ala Thr Cys Cys Asn Gly

450 455 460

Ser Ser Ser Leu Asp Val Glu Lys Leu Ala Asn Glu Ile Lys Lys Leu

465 470 475 480

Ser Arg Asn

<210> 119

<211> 1404

<212> DNA

<213> Stevia rebaudiana

<220>

<221> CDS

<222> (1)..(1404)

<400> 119

atg cca atc tcc gat atc aat gct ggt tcc cac att ttg gtt ttc cca 48

Met Pro Ile Ser Asp Ile Asn Ala Gly Ser His Ile Leu Val Phe Pro

1 5 10 15

tac cca gct caa ggt cac atg ttg act cta tta gat ttg acc cac caa 96

Tyr Pro Ala Gln Gly His Met Leu Thr Leu Leu Asp Leu Thr His Gln

20 25 30

ttg gcc atc aga aac ttg act atc act atc tta gtc acc cca aag aac 144

Leu Ala Ile Arg Asn Leu Thr Ile Thr Ile Leu Val Thr Pro Lys Asn

35 40 45

ttg cca acc att tct cca ttg ttg gct gct cat cca acc act gtt tct 192

Leu Pro Thr Ile Ser Pro Leu Leu Ala Ala His Pro Thr Thr Val Ser

50 55 60

gct ttg ttg ttg cca tta cca cct cac cca gcc atc cca tct ggt att 240

Ala Leu Leu Leu Pro Leu Pro Pro His Pro Ala Ile Pro Ser Gly Ile

65 70 75 80

gaa aac gtc aag gac ttg cca aac gat gct ttc aag gct atg atg gtt 288

Glu Asn Val Lys Asp Leu Pro Asn Asp Ala Phe Lys Ala Met Met Val

85 90 95

gct tta ggt gac ttg tac aac cca ttg aga gac tgg ttc aga aac caa 336

Ala Leu Gly Asp Leu Tyr Asn Pro Leu Arg Asp Trp Phe Arg Asn Gln

100 105 110

cct aac cca cca gtt gcc atc att tct gac ttc ttc ttg ggt tgg act 384

Pro Asn Pro Pro Val Ala Ile Ile Ser Asp Phe Phe Leu Gly Trp Thr

115 120 125

cac cac ttg gct gtc gaa tta ggt atc aga aga tac act ttc tct cca 432

His His Leu Ala Val Glu Leu Gly Ile Arg Arg Tyr Thr Phe Ser Pro

130 135 140

tct ggt gct ttg gct ttg tcc gtc att ttc tct tta tgg aga tac caa 480

Ser Gly Ala Leu Ala Leu Ser Val Ile Phe Ser Leu Trp Arg Tyr Gln

145 150 155 160

cca aag aga atc gat gtc gaa aac gaa aag gaa gct atc aaa ttc cca 528

Pro Lys Arg Ile Asp Val Glu Asn Glu Lys Glu Ala Ile Lys Phe Pro

165 170 175

aag att cca aac tct cca gaa tac cca tgg tgg caa tta tct cca atc 576

Lys Ile Pro Asn Ser Pro Glu Tyr Pro Trp Trp Gln Leu Ser Pro Ile

180 185 190

tac aga tct tac gtt gaa ggt gac cca gat tct gaa ttc atc aag gat 624

Tyr Arg Ser Tyr Val Glu Gly Asp Pro Asp Ser Glu Phe Ile Lys Asp

195 200 205

ggt ttc ttg gct gat att gct tcc tgg ggt att gtc atc aac tct ttc 672

Gly Phe Leu Ala Asp Ile Ala Ser Trp Gly Ile Val Ile Asn Ser Phe

210 215 220

acc gaa ttg gaa caa gtc tac gtt gac cat ttg aag cat gaa ttg ggt 720

Thr Glu Leu Glu Gln Val Tyr Val Asp His Leu Lys His Glu Leu Gly

225 230 235 240

cac gac caa gtc ttt gct gtc ggt cca tta ttg cct cca ggt gac aag 768

His Asp Gln Val Phe Ala Val Gly Pro Leu Leu Pro Pro Gly Asp Lys

245 250 255

act tct ggt aga ggt ggt tct tct tcc aac gat gtc ttg tcc tgg ttg 816

Thr Ser Gly Arg Gly Gly Ser Ser Ser Asn Asp Val Leu Ser Trp Leu

260 265 270

gac acc tgt gct gac aga acc gtt gtc tac gtt tgt ttc ggt tct caa 864

Asp Thr Cys Ala Asp Arg Thr Val Val Tyr Val Cys Phe Gly Ser Gln

275 280 285

atg gtt ttg acc aac ggt caa atg gaa gtt gtt gct ttg ggt ttg gaa 912

Met Val Leu Thr Asn Gly Gln Met Glu Val Val Ala Leu Gly Leu Glu

290 295 300

aag tcc cgt gtc aaa ttt gtc tgg tcc gtc aag gaa cca act gtt ggt 960

Lys Ser Arg Val Lys Phe Val Trp Ser Val Lys Glu Pro Thr Val Gly

305 310 315 320

cac gaa gct gct aac tac ggt cgt gtt cca cca ggt ttc gaa gac aga 1008

His Glu Ala Ala Asn Tyr Gly Arg Val Pro Pro Gly Phe Glu Asp Arg

325 330 335

gtt tcc ggt cgt ggt ttg gtt atc aga ggt tgg gtt cca caa gtt gcc 1056

Val Ser Gly Arg Gly Leu Val Ile Arg Gly Trp Val Pro Gln Val Ala

340 345 350

att ttg tct cac gac tcc gtt ggt gtt ttc ttg acc cac tgt ggt tgg 1104

Ile Leu Ser His Asp Ser Val Gly Val Phe Leu Thr His Cys Gly Trp

355 360 365

aac tct gtc atg gaa gct gtt gct gct gaa gtt ttg atg ttg acc tgg 1152

Asn Ser Val Met Glu Ala Val Ala Ala Glu Val Leu Met Leu Thr Trp

370 375 380

cca atg tcc gct gac caa ttc tcc aat gcc act ttg ttg cac gaa ttg 1200

Pro Met Ser Ala Asp Gln Phe Ser Asn Ala Thr Leu Leu His Glu Leu

385 390 395 400

aag gtt ggt atc aag gtt tgt gaa ggt tcc aac att gtc cca aac tct 1248

Lys Val Gly Ile Lys Val Cys Glu Gly Ser Asn Ile Val Pro Asn Ser

405 410 415

gac gaa ttg gct gaa ttg ttc tcc aaa tct cta tcc gat gaa act aga 1296

Asp Glu Leu Ala Glu Leu Phe Ser Lys Ser Leu Ser Asp Glu Thr Arg

420 425 430

ttg gaa aga aag aga gtc aag gaa ttt gcc aaa tct gcc aag gaa gcc 1344

Leu Glu Arg Lys Arg Val Lys Glu Phe Ala Lys Ser Ala Lys Glu Ala

435 440 445

gtc ggt cca aag ggt tcc tct gtc ggt gaa tta gaa aga ttg gtt gac 1392

Val Gly Pro Lys Gly Ser Ser Val Gly Glu Leu Glu Arg Leu Val Asp

450 455 460

aac ttg tct ttg 1404

Asn Leu Ser Leu

465

<210> 120

<211> 468

<212> PRT

<213> Stevia rebaudiana

<400> 120

Met Pro Ile Ser Asp Ile Asn Ala Gly Ser His Ile Leu Val Phe Pro

1 5 10 15

Tyr Pro Ala Gln Gly His Met Leu Thr Leu Leu Asp Leu Thr His Gln

20 25 30

Leu Ala Ile Arg Asn Leu Thr Ile Thr Ile Leu Val Thr Pro Lys Asn

35 40 45

Leu Pro Thr Ile Ser Pro Leu Leu Ala Ala His Pro Thr Thr Val Ser

50 55 60

Ala Leu Leu Leu Pro Leu Pro Pro His Pro Ala Ile Pro Ser Gly Ile

65 70 75 80

Glu Asn Val Lys Asp Leu Pro Asn Asp Ala Phe Lys Ala Met Met Val

85 90 95

Ala Leu Gly Asp Leu Tyr Asn Pro Leu Arg Asp Trp Phe Arg Asn Gln

100 105 110

Pro Asn Pro Pro Val Ala Ile Ile Ser Asp Phe Phe Leu Gly Trp Thr

115 120 125

His His Leu Ala Val Glu Leu Gly Ile Arg Arg Tyr Thr Phe Ser Pro

130 135 140

Ser Gly Ala Leu Ala Leu Ser Val Ile Phe Ser Leu Trp Arg Tyr Gln

145 150 155 160

Pro Lys Arg Ile Asp Val Glu Asn Glu Lys Glu Ala Ile Lys Phe Pro

165 170 175

Lys Ile Pro Asn Ser Pro Glu Tyr Pro Trp Trp Gln Leu Ser Pro Ile

180 185 190

Tyr Arg Ser Tyr Val Glu Gly Asp Pro Asp Ser Glu Phe Ile Lys Asp

195 200 205

Gly Phe Leu Ala Asp Ile Ala Ser Trp Gly Ile Val Ile Asn Ser Phe

210 215 220

Thr Glu Leu Glu Gln Val Tyr Val Asp His Leu Lys His Glu Leu Gly

225 230 235 240

His Asp Gln Val Phe Ala Val Gly Pro Leu Leu Pro Pro Gly Asp Lys

245 250 255

Thr Ser Gly Arg Gly Gly Ser Ser Ser Asn Asp Val Leu Ser Trp Leu

260 265 270

Asp Thr Cys Ala Asp Arg Thr Val Val Tyr Val Cys Phe Gly Ser Gln

275 280 285

Met Val Leu Thr Asn Gly Gln Met Glu Val Val Ala Leu Gly Leu Glu

290 295 300

Lys Ser Arg Val Lys Phe Val Trp Ser Val Lys Glu Pro Thr Val Gly

305 310 315 320

His Glu Ala Ala Asn Tyr Gly Arg Val Pro Pro Gly Phe Glu Asp Arg

325 330 335

Val Ser Gly Arg Gly Leu Val Ile Arg Gly Trp Val Pro Gln Val Ala

340 345 350

Ile Leu Ser His Asp Ser Val Gly Val Phe Leu Thr His Cys Gly Trp

355 360 365

Asn Ser Val Met Glu Ala Val Ala Ala Glu Val Leu Met Leu Thr Trp

370 375 380

Pro Met Ser Ala Asp Gln Phe Ser Asn Ala Thr Leu Leu His Glu Leu

385 390 395 400

Lys Val Gly Ile Lys Val Cys Glu Gly Ser Asn Ile Val Pro Asn Ser

405 410 415

Asp Glu Leu Ala Glu Leu Phe Ser Lys Ser Leu Ser Asp Glu Thr Arg

420 425 430

Leu Glu Arg Lys Arg Val Lys Glu Phe Ala Lys Ser Ala Lys Glu Ala

435 440 445

Val Gly Pro Lys Gly Ser Ser Val Gly Glu Leu Glu Arg Leu Val Asp

450 455 460

Asn Leu Ser Leu

465

<210> 121

<211> 1383

<212> DNA

<213> Stevia rebaudiana

<220>

<221> CDS

<222> (1)..(1383)

<400> 121

atg gaa tct tcc aag gtc att ttg tac cca tct cca ggt att ggt cac 48

Met Glu Ser Ser Lys Val Ile Leu Tyr Pro Ser Pro Gly Ile Gly His

1 5 10 15

ttg gtt tcc atg gtc gaa ttg ggt aaa ttg att cac acc cac cac cca 96

Leu Val Ser Met Val Glu Leu Gly Lys Leu Ile His Thr His His Pro

20 25 30

tct cta tcc gtc atc atc ttg gtt ttg cca gct acc tac gaa act ggt 144

Ser Leu Ser Val Ile Ile Leu Val Leu Pro Ala Thr Tyr Glu Thr Gly

35 40 45

tcc acc acc acc tac atc aac act gtt tct acc acc act cca ttc atc 192

Ser Thr Thr Thr Tyr Ile Asn Thr Val Ser Thr Thr Thr Pro Phe Ile

50 55 60

act ttc cac cac ttg cca gtc att cca ttg cca cca gac tcc tct tct 240

Thr Phe His His Leu Pro Val Ile Pro Leu Pro Pro Asp Ser Ser Ser

65 70 75 80

gaa ttt atc gat ttg gct ttc gac att cca caa tta tac aac cca gtt 288

Glu Phe Ile Asp Leu Ala Phe Asp Ile Pro Gln Leu Tyr Asn Pro Val

85 90 95

gtc tac aac act ttg gtt gcc atc tct gaa act tcc acc atc aag gct 336

Val Tyr Asn Thr Leu Val Ala Ile Ser Glu Thr Ser Thr Ile Lys Ala

100 105 110

gtt atc ttg gac ttc ttt gtc aac gcc gct ttc caa atc tcc aag tct 384

Val Ile Leu Asp Phe Phe Val Asn Ala Ala Phe Gln Ile Ser Lys Ser

115 120 125

ttg gac ttg cca acc tac tac ttc ttc act tct ggt gct tct ggt cta 432

Leu Asp Leu Pro Thr Tyr Tyr Phe Phe Thr Ser Gly Ala Ser Gly Leu

130 135 140

tgt gct ttc ttg cat ttg cca act atc tac aag acc tac tcc ggt aac 480

Cys Ala Phe Leu His Leu Pro Thr Ile Tyr Lys Thr Tyr Ser Gly Asn

145 150 155 160

ttc aag gac ttg gac act ttc atc aac att cca ggt gtt cct cct atc 528

Phe Lys Asp Leu Asp Thr Phe Ile Asn Ile Pro Gly Val Pro Pro Ile

165 170 175

cac tct tct gac atg cca acc gtc tta ttc gac aag gaa tcc aac tct 576

His Ser Ser Asp Met Pro Thr Val Leu Phe Asp Lys Glu Ser Asn Ser

180 185 190

tac aag aac ttt gtc aag act tcc aac aac atg gcc aaa tct tct ggt 624

Tyr Lys Asn Phe Val Lys Thr Ser Asn Asn Met Ala Lys Ser Ser Gly

195 200 205

gtc att gct aac tcc ttc ttg caa ttg gaa gaa aga gct gct caa act 672

Val Ile Ala Asn Ser Phe Leu Gln Leu Glu Glu Arg Ala Ala Gln Thr

210 215 220

ttg aga gat ggt aag tcc atc acc gat ggt cca tct cca cca atc tac 720

Leu Arg Asp Gly Lys Ser Ile Thr Asp Gly Pro Ser Pro Pro Ile Tyr

225 230 235 240

ttg att ggt cca ttg atc gct tct ggt aac caa gtt gac cat aat gaa 768

Leu Ile Gly Pro Leu Ile Ala Ser Gly Asn Gln Val Asp His Asn Glu

245 250 255

aac gaa tgt ttg aaa tgg tta aac act caa cca tcc aaa tct gtt gtt 816

Asn Glu Cys Leu Lys Trp Leu Asn Thr Gln Pro Ser Lys Ser Val Val

260 265 270

ttc ttg tgt ttc ggt tct caa ggt gtt ttc aag aag gaa caa ttg aag 864

Phe Leu Cys Phe Gly Ser Gln Gly Val Phe Lys Lys Glu Gln Leu Lys

275 280 285

gaa att gct gtt ggt ttg gaa aga tct ggt caa aga ttc tta tgg gtt 912

Glu Ile Ala Val Gly Leu Glu Arg Ser Gly Gln Arg Phe Leu Trp Val

290 295 300

gtc aga aag cct cca tct gat ggt ggt aag gaa ttc ggt ttg gat gac 960

Val Arg Lys Pro Pro Ser Asp Gly Gly Lys Glu Phe Gly Leu Asp Asp

305 310 315 320

gtc ttg cca gaa ggt ttc gtt gcc aga acc aag gaa aag ggt ttg gtt 1008

Val Leu Pro Glu Gly Phe Val Ala Arg Thr Lys Glu Lys Gly Leu Val

325 330 335

gtc aag aac tgg gct cca caa cca gct att ttg ggt cac gaa tct gtc 1056

Val Lys Asn Trp Ala Pro Gln Pro Ala Ile Leu Gly His Glu Ser Val

340 345 350

ggt ggt ttc gtt tcc cac tgt ggt tgg aac tct tct ttg gaa gcc gtt 1104

Gly Gly Phe Val Ser His Cys Gly Trp Asn Ser Ser Leu Glu Ala Val

355 360 365

gtt ttc ggt gtc cca atg gtt gct tgg cca tta tac gct gaa caa aag 1152

Val Phe Gly Val Pro Met Val Ala Trp Pro Leu Tyr Ala Glu Gln Lys

370 375 380

atg aac cgt gtc tac ttg gtc gaa gaa atc aag gtt gct tta tgg tta 1200

Met Asn Arg Val Tyr Leu Val Glu Glu Ile Lys Val Ala Leu Trp Leu

385 390 395 400

aga atg tcc gct gat ggt ttt gtc tct gct gaa gct gtt gaa gaa act 1248

Arg Met Ser Ala Asp Gly Phe Val Ser Ala Glu Ala Val Glu Glu Thr

405 410 415

gtc aga caa ttg atg gac ggt aga aga gtt cgt gaa aga att ttg gaa 1296

Val Arg Gln Leu Met Asp Gly Arg Arg Val Arg Glu Arg Ile Leu Glu

420 425 430

atg tcc acc aag gcc aag gcc gct gtc gaa gat ggt ggt tct tcc aga 1344

Met Ser Thr Lys Ala Lys Ala Ala Val Glu Asp Gly Gly Ser Ser Arg

435 440 445

gtt gac ttc ttc aaa ttg act gaa tcc tgg acc cac aag 1383

Val Asp Phe Phe Lys Leu Thr Glu Ser Trp Thr His Lys

450 455 460

<210> 122

<211> 461

<212> PRT

<213> Stevia rebaudiana

<400> 122

Met Glu Ser Ser Lys Val Ile Leu Tyr Pro Ser Pro Gly Ile Gly His

1 5 10 15

Leu Val Ser Met Val Glu Leu Gly Lys Leu Ile His Thr His His Pro

20 25 30

Ser Leu Ser Val Ile Ile Leu Val Leu Pro Ala Thr Tyr Glu Thr Gly

35 40 45

Ser Thr Thr Thr Tyr Ile Asn Thr Val Ser Thr Thr Thr Pro Phe Ile

50 55 60

Thr Phe His His Leu Pro Val Ile Pro Leu Pro Pro Asp Ser Ser Ser

65 70 75 80

Glu Phe Ile Asp Leu Ala Phe Asp Ile Pro Gln Leu Tyr Asn Pro Val

85 90 95

Val Tyr Asn Thr Leu Val Ala Ile Ser Glu Thr Ser Thr Ile Lys Ala

100 105 110

Val Ile Leu Asp Phe Phe Val Asn Ala Ala Phe Gln Ile Ser Lys Ser

115 120 125

Leu Asp Leu Pro Thr Tyr Tyr Phe Phe Thr Ser Gly Ala Ser Gly Leu

130 135 140

Cys Ala Phe Leu His Leu Pro Thr Ile Tyr Lys Thr Tyr Ser Gly Asn

145 150 155 160

Phe Lys Asp Leu Asp Thr Phe Ile Asn Ile Pro Gly Val Pro Pro Ile

165 170 175

His Ser Ser Asp Met Pro Thr Val Leu Phe Asp Lys Glu Ser Asn Ser

180 185 190

Tyr Lys Asn Phe Val Lys Thr Ser Asn Asn Met Ala Lys Ser Ser Gly

195 200 205

Val Ile Ala Asn Ser Phe Leu Gln Leu Glu Glu Arg Ala Ala Gln Thr

210 215 220

Leu Arg Asp Gly Lys Ser Ile Thr Asp Gly Pro Ser Pro Pro Ile Tyr

225 230 235 240

Leu Ile Gly Pro Leu Ile Ala Ser Gly Asn Gln Val Asp His Asn Glu

245 250 255

Asn Glu Cys Leu Lys Trp Leu Asn Thr Gln Pro Ser Lys Ser Val Val

260 265 270

Phe Leu Cys Phe Gly Ser Gln Gly Val Phe Lys Lys Glu Gln Leu Lys

275 280 285

Glu Ile Ala Val Gly Leu Glu Arg Ser Gly Gln Arg Phe Leu Trp Val

290 295 300

Val Arg Lys Pro Pro Ser Asp Gly Gly Lys Glu Phe Gly Leu Asp Asp

305 310 315 320

Val Leu Pro Glu Gly Phe Val Ala Arg Thr Lys Glu Lys Gly Leu Val

325 330 335

Val Lys Asn Trp Ala Pro Gln Pro Ala Ile Leu Gly His Glu Ser Val

340 345 350

Gly Gly Phe Val Ser His Cys Gly Trp Asn Ser Ser Leu Glu Ala Val

355 360 365

Val Phe Gly Val Pro Met Val Ala Trp Pro Leu Tyr Ala Glu Gln Lys

370 375 380

Met Asn Arg Val Tyr Leu Val Glu Glu Ile Lys Val Ala Leu Trp Leu

385 390 395 400

Arg Met Ser Ala Asp Gly Phe Val Ser Ala Glu Ala Val Glu Glu Thr

405 410 415

Val Arg Gln Leu Met Asp Gly Arg Arg Val Arg Glu Arg Ile Leu Glu

420 425 430

Met Ser Thr Lys Ala Lys Ala Ala Val Glu Asp Gly Gly Ser Ser Arg

435 440 445

Val Asp Phe Phe Lys Leu Thr Glu Ser Trp Thr His Lys

450 455 460

<210> 123

<211> 1455

<212> DNA

<213> Stevia rebaudiana

<220>

<221> CDS

<222> (1)..(1455)

<400> 123

atg tac aac gtt act tac cat caa aac tcc aag gcc atg gct acc tct 48

Met Tyr Asn Val Thr Tyr His Gln Asn Ser Lys Ala Met Ala Thr Ser

1 5 10 15

gac tcc att gtc gac gac aga aag caa ttg cac gtt gcc act ttc cca 96

Asp Ser Ile Val Asp Asp Arg Lys Gln Leu His Val Ala Thr Phe Pro

20 25 30

tgg ttg gct ttc ggt cac atc ttg cca ttc ttg caa ttg tcc aaa ttg 144

Trp Leu Ala Phe Gly His Ile Leu Pro Phe Leu Gln Leu Ser Lys Leu

35 40 45

att gct gaa aag ggt cac aaa gtc tct ttc ttg tct acc acc aga aac 192

Ile Ala Glu Lys Gly His Lys Val Ser Phe Leu Ser Thr Thr Arg Asn

50 55 60

atc caa aga ttg tcc tct cac atc tct cca ttg att aac gtt gtc caa 240

Ile Gln Arg Leu Ser Ser His Ile Ser Pro Leu Ile Asn Val Val Gln

65 70 75 80

ttg act tta cca aga gtc caa gaa ttg cca gaa gat gct gaa gct act 288

Leu Thr Leu Pro Arg Val Gln Glu Leu Pro Glu Asp Ala Glu Ala Thr

85 90 95

act gat gtt cac cca gaa gat atc caa tac ttg aag aag gct gtc gat 336

Thr Asp Val His Pro Glu Asp Ile Gln Tyr Leu Lys Lys Ala Val Asp

100 105 110

ggt ttg caa cca gaa gtt acc aga ttc ttg gaa caa cac tct cca gac 384

Gly Leu Gln Pro Glu Val Thr Arg Phe Leu Glu Gln His Ser Pro Asp

115 120 125

tgg atc atc tac gac ttc act cac tac tgg tta cca tcc att gct gct 432

Trp Ile Ile Tyr Asp Phe Thr His Tyr Trp Leu Pro Ser Ile Ala Ala

130 135 140

tct ttg ggt atc tcc aga gct tac ttc tgt gtt atc act cca tgg acc 480

Ser Leu Gly Ile Ser Arg Ala Tyr Phe Cys Val Ile Thr Pro Trp Thr

145 150 155 160

att gct tac ttg gct cca tct tct gat gcc atg atc aac gac tct gat 528

Ile Ala Tyr Leu Ala Pro Ser Ser Asp Ala Met Ile Asn Asp Ser Asp

165 170 175

ggt aga acc act gtt gaa gat ttg acc acc cca cct aaa tgg ttc cca 576

Gly Arg Thr Thr Val Glu Asp Leu Thr Thr Pro Pro Lys Trp Phe Pro

180 185 190

ttc cca acc aag gtc tgt tgg aga aag cat gat ttg gcc aga atg gaa 624

Phe Pro Thr Lys Val Cys Trp Arg Lys His Asp Leu Ala Arg Met Glu

195 200 205

cca tac gaa gct cca ggt atc tct gac ggt tac aga atg ggt atg gtt 672

Pro Tyr Glu Ala Pro Gly Ile Ser Asp Gly Tyr Arg Met Gly Met Val

210 215 220

ttc aag ggt tct gac tgt ttg ttg ttc aaa tgt tac cat gaa ttc ggt 720

Phe Lys Gly Ser Asp Cys Leu Leu Phe Lys Cys Tyr His Glu Phe Gly

225 230 235 240

act caa tgg tta cct cta tta gaa act ttg cac caa gtt cca gtt gtt 768

Thr Gln Trp Leu Pro Leu Leu Glu Thr Leu His Gln Val Pro Val Val

245 250 255

cca gtc ggt ttg ttg cct cca gaa atc cca ggt gac gaa aag gac gaa 816

Pro Val Gly Leu Leu Pro Pro Glu Ile Pro Gly Asp Glu Lys Asp Glu

260 265 270

acc tgg gtt tcc atc aag aaa tgg ttg gat ggt aag caa aag ggt tcc 864

Thr Trp Val Ser Ile Lys Lys Trp Leu Asp Gly Lys Gln Lys Gly Ser

275 280 285

gtt gtc tac gtt gct tta ggt tct gaa gct tta gtc tct caa act gaa 912

Val Val Tyr Val Ala Leu Gly Ser Glu Ala Leu Val Ser Gln Thr Glu

290 295 300

gtt gtc gaa ttg gct ttg ggt ttg gaa ttg tcc ggt ttg cca ttt gtc 960

Val Val Glu Leu Ala Leu Gly Leu Glu Leu Ser Gly Leu Pro Phe Val

305 310 315 320

tgg gct tac aga aag cca aag ggt cca gcc aag tct gat tct gtc gaa 1008

Trp Ala Tyr Arg Lys Pro Lys Gly Pro Ala Lys Ser Asp Ser Val Glu

325 330 335

tta cca gat ggt ttc gtc gaa aga acc cgt gac cgt ggt ttg gtc tgg 1056

Leu Pro Asp Gly Phe Val Glu Arg Thr Arg Asp Arg Gly Leu Val Trp

340 345 350

act tcc tgg gct cca caa ttg aga att ttg tcc cac gaa tct gtc tgt 1104

Thr Ser Trp Ala Pro Gln Leu Arg Ile Leu Ser His Glu Ser Val Cys

355 360 365

ggt ttc ttg act cac tgt ggt tct ggt tcc att gtt gaa ggt ttg atg 1152

Gly Phe Leu Thr His Cys Gly Ser Gly Ser Ile Val Glu Gly Leu Met

370 375 380

ttc ggt cac cca ttg atc atg ttg cca atc ttc tgt gac caa cca tta 1200

Phe Gly His Pro Leu Ile Met Leu Pro Ile Phe Cys Asp Gln Pro Leu

385 390 395 400

aac gct aga ttg ttg gaa gac aag caa gtc ggt att gaa att cca aga 1248

Asn Ala Arg Leu Leu Glu Asp Lys Gln Val Gly Ile Glu Ile Pro Arg

405 410 415

aac gaa gaa gac ggt tgt ttg acc aag gaa tcc gtt gcc aga tct ttg 1296

Asn Glu Glu Asp Gly Cys Leu Thr Lys Glu Ser Val Ala Arg Ser Leu

420 425 430

aga tct gtt gtt gtc gaa aac gaa ggt gaa atc tac aag gct aac gct 1344

Arg Ser Val Val Val Glu Asn Glu Gly Glu Ile Tyr Lys Ala Asn Ala

435 440 445

cgt gct cta tcc aag atc tac aac gac acc aag gtc gaa aag gaa tac 1392

Arg Ala Leu Ser Lys Ile Tyr Asn Asp Thr Lys Val Glu Lys Glu Tyr

450 455 460

gtt tct caa ttt gtt gac tac ttg gaa aag aac gcc aga gct gtt gcc 1440

Val Ser Gln Phe Val Asp Tyr Leu Glu Lys Asn Ala Arg Ala Val Ala

465 470 475 480

att gac cac gaa agt 1455

Ile Asp His Glu Ser

485

<210> 124

<211> 485

<212> PRT

<213> Stevia rebaudiana

<400> 124

Met Tyr Asn Val Thr Tyr His Gln Asn Ser Lys Ala Met Ala Thr Ser

1 5 10 15

Asp Ser Ile Val Asp Asp Arg Lys Gln Leu His Val Ala Thr Phe Pro

20 25 30

Trp Leu Ala Phe Gly His Ile Leu Pro Phe Leu Gln Leu Ser Lys Leu

35 40 45

Ile Ala Glu Lys Gly His Lys Val Ser Phe Leu Ser Thr Thr Arg Asn

50 55 60

Ile Gln Arg Leu Ser Ser His Ile Ser Pro Leu Ile Asn Val Val Gln

65 70 75 80

Leu Thr Leu Pro Arg Val Gln Glu Leu Pro Glu Asp Ala Glu Ala Thr

85 90 95

Thr Asp Val His Pro Glu Asp Ile Gln Tyr Leu Lys Lys Ala Val Asp

100 105 110

Gly Leu Gln Pro Glu Val Thr Arg Phe Leu Glu Gln His Ser Pro Asp

115 120 125

Trp Ile Ile Tyr Asp Phe Thr His Tyr Trp Leu Pro Ser Ile Ala Ala

130 135 140

Ser Leu Gly Ile Ser Arg Ala Tyr Phe Cys Val Ile Thr Pro Trp Thr

145 150 155 160

Ile Ala Tyr Leu Ala Pro Ser Ser Asp Ala Met Ile Asn Asp Ser Asp

165 170 175

Gly Arg Thr Thr Val Glu Asp Leu Thr Thr Pro Pro Lys Trp Phe Pro

180 185 190

Phe Pro Thr Lys Val Cys Trp Arg Lys His Asp Leu Ala Arg Met Glu

195 200 205

Pro Tyr Glu Ala Pro Gly Ile Ser Asp Gly Tyr Arg Met Gly Met Val

210 215 220

Phe Lys Gly Ser Asp Cys Leu Leu Phe Lys Cys Tyr His Glu Phe Gly

225 230 235 240

Thr Gln Trp Leu Pro Leu Leu Glu Thr Leu His Gln Val Pro Val Val

245 250 255

Pro Val Gly Leu Leu Pro Pro Glu Ile Pro Gly Asp Glu Lys Asp Glu

260 265 270

Thr Trp Val Ser Ile Lys Lys Trp Leu Asp Gly Lys Gln Lys Gly Ser

275 280 285

Val Val Tyr Val Ala Leu Gly Ser Glu Ala Leu Val Ser Gln Thr Glu

290 295 300

Val Val Glu Leu Ala Leu Gly Leu Glu Leu Ser Gly Leu Pro Phe Val

305 310 315 320

Trp Ala Tyr Arg Lys Pro Lys Gly Pro Ala Lys Ser Asp Ser Val Glu

325 330 335

Leu Pro Asp Gly Phe Val Glu Arg Thr Arg Asp Arg Gly Leu Val Trp

340 345 350

Thr Ser Trp Ala Pro Gln Leu Arg Ile Leu Ser His Glu Ser Val Cys

355 360 365

Gly Phe Leu Thr His Cys Gly Ser Gly Ser Ile Val Glu Gly Leu Met

370 375 380

Phe Gly His Pro Leu Ile Met Leu Pro Ile Phe Cys Asp Gln Pro Leu

385 390 395 400

Asn Ala Arg Leu Leu Glu Asp Lys Gln Val Gly Ile Glu Ile Pro Arg

405 410 415

Asn Glu Glu Asp Gly Cys Leu Thr Lys Glu Ser Val Ala Arg Ser Leu

420 425 430

Arg Ser Val Val Val Glu Asn Glu Gly Glu Ile Tyr Lys Ala Asn Ala

435 440 445

Arg Ala Leu Ser Lys Ile Tyr Asn Asp Thr Lys Val Glu Lys Glu Tyr

450 455 460

Val Ser Gln Phe Val Asp Tyr Leu Glu Lys Asn Ala Arg Ala Val Ala

465 470 475 480

Ile Asp His Glu Ser

485

<210> 125

<211> 1485

<212> DNA

<213> Stevia rebaudiana

<220>

<221> CDS

<222> (1)..(1485)

<400> 125

atg tct cca aag atg gtt gct cca cca acc aac ttg cac ttc gtc tta 48

Met Ser Pro Lys Met Val Ala Pro Pro Thr Asn Leu His Phe Val Leu

1 5 10 15

ttc cca ttg atg gct caa ggt cac ttg gtt cca atg gtc gac att gct 96

Phe Pro Leu Met Ala Gln Gly His Leu Val Pro Met Val Asp Ile Ala

20 25 30

aga att ttg gct caa aga ggt gct acc gtc acc atc atc acc act cca 144

Arg Ile Leu Ala Gln Arg Gly Ala Thr Val Thr Ile Ile Thr Thr Pro

35 40 45

tac cat gcc aac aga gtc aga cca gtt atc tcc aga gcc att gct acc 192

Tyr His Ala Asn Arg Val Arg Pro Val Ile Ser Arg Ala Ile Ala Thr

50 55 60

aac ttg aaa atc caa ttg ttg gaa ttg caa ttg aga tcc act gaa gct 240

Asn Leu Lys Ile Gln Leu Leu Glu Leu Gln Leu Arg Ser Thr Glu Ala

65 70 75 80

ggt ttg cca gaa ggt tgt gaa tct ttc gac caa ttg cca tct ttc gaa 288

Gly Leu Pro Glu Gly Cys Glu Ser Phe Asp Gln Leu Pro Ser Phe Glu

85 90 95

tac tgg aag aac atc tcc act gcc att gac ttg ttg caa caa cca gct 336

Tyr Trp Lys Asn Ile Ser Thr Ala Ile Asp Leu Leu Gln Gln Pro Ala

100 105 110

gaa gat cta ttg aga gaa ttg tct cca cct cca gac tgt atc atc tcc 384

Glu Asp Leu Leu Arg Glu Leu Ser Pro Pro Pro Asp Cys Ile Ile Ser

115 120 125

gat ttc ttg ttc cca tgg acc act gat gtc gcc aga aga ttg aac att 432

Asp Phe Leu Phe Pro Trp Thr Thr Asp Val Ala Arg Arg Leu Asn Ile

130 135 140

cca aga ttg gtt ttc aac ggt cca ggt tgt ttc tac ttg ttg tgt atc 480

Pro Arg Leu Val Phe Asn Gly Pro Gly Cys Phe Tyr Leu Leu Cys Ile

145 150 155 160

cac gtt gcc atc act tct aac atc tta ggt gaa aac gaa cct gtt tcc 528

His Val Ala Ile Thr Ser Asn Ile Leu Gly Glu Asn Glu Pro Val Ser

165 170 175

tcc aac act gaa aga gtt gtt ttg cca ggt tta cca gat cgt att gaa 576

Ser Asn Thr Glu Arg Val Val Leu Pro Gly Leu Pro Asp Arg Ile Glu

180 185 190

gtt acc aaa ttg caa atc gtc ggt tct tcc aga cca gct aac gtt gac 624

Val Thr Lys Leu Gln Ile Val Gly Ser Ser Arg Pro Ala Asn Val Asp

195 200 205

gaa atg ggt tcc tgg tta aga gct gtc gaa gct gaa aag gct tct ttc 672

Glu Met Gly Ser Trp Leu Arg Ala Val Glu Ala Glu Lys Ala Ser Phe

210 215 220

ggt att gtc gtc aac acc ttt gaa gaa ttg gaa cca gaa tac gtc gaa 720

Gly Ile Val Val Asn Thr Phe Glu Glu Leu Glu Pro Glu Tyr Val Glu

225 230 235 240

gaa tac aag acc gtc aag gac aag aag atg tgg tgt atc ggt cca gtt 768

Glu Tyr Lys Thr Val Lys Asp Lys Lys Met Trp Cys Ile Gly Pro Val

245 250 255

tct cta tgt aac aag act ggt cca gac ttg gct gaa aga ggt aac aag 816

Ser Leu Cys Asn Lys Thr Gly Pro Asp Leu Ala Glu Arg Gly Asn Lys

260 265 270

gct gcc atc acc gaa cac aac tgt ttg aaa tgg ttg gat gaa aga aag 864

Ala Ala Ile Thr Glu His Asn Cys Leu Lys Trp Leu Asp Glu Arg Lys

275 280 285

ttg ggt tct gtc ttg tac gtt tgt ttg ggt tct ttg gcc aga atc tcc 912

Leu Gly Ser Val Leu Tyr Val Cys Leu Gly Ser Leu Ala Arg Ile Ser

290 295 300

gct gct caa gcc att gaa tta ggt tta ggt ttg gaa tct atc aac aga 960

Ala Ala Gln Ala Ile Glu Leu Gly Leu Gly Leu Glu Ser Ile Asn Arg

305 310 315 320

cca ttt atc tgg tgt gtt aga aac gaa act gat gaa ttg aag act tgg 1008

Pro Phe Ile Trp Cys Val Arg Asn Glu Thr Asp Glu Leu Lys Thr Trp

325 330 335

ttc ttg gac ggt ttc gaa gaa aga gtt cgt gac aga ggt ttg att gtt 1056

Phe Leu Asp Gly Phe Glu Glu Arg Val Arg Asp Arg Gly Leu Ile Val

340 345 350

cac ggt tgg gct cca caa gtt ttg atc tta tct cac cca acc att ggt 1104

His Gly Trp Ala Pro Gln Val Leu Ile Leu Ser His Pro Thr Ile Gly

355 360 365

ggt ttc ttg act cac tgt ggt tgg aac tct acc att gaa tcc atc act 1152

Gly Phe Leu Thr His Cys Gly Trp Asn Ser Thr Ile Glu Ser Ile Thr

370 375 380

gct ggt gtt cca atg atc acc tgg cca ttc ttt gct gac caa ttc ttg 1200

Ala Gly Val Pro Met Ile Thr Trp Pro Phe Phe Ala Asp Gln Phe Leu

385 390 395 400

aat gaa gct ttc atc gtt gaa gtt ttg aaa att ggt gtc aga atc ggt 1248

Asn Glu Ala Phe Ile Val Glu Val Leu Lys Ile Gly Val Arg Ile Gly

405 410 415

gtt gaa aga gct tgt ttg ttc ggt gaa gaa gac aag gtc ggt gtc tta 1296

Val Glu Arg Ala Cys Leu Phe Gly Glu Glu Asp Lys Val Gly Val Leu

420 425 430

gtc aag aag gaa gat gtt aag aag gct gtt gaa tgt ttg atg gac gaa 1344

Val Lys Lys Glu Asp Val Lys Lys Ala Val Glu Cys Leu Met Asp Glu

435 440 445

gat gaa gac ggt gac caa aga aga aag cgt gtc att gaa ttg gcc aag 1392

Asp Glu Asp Gly Asp Gln Arg Arg Lys Arg Val Ile Glu Leu Ala Lys

450 455 460

atg gct aag att gct atg gct gaa ggt ggt tct tct tac gaa aac gtt 1440

Met Ala Lys Ile Ala Met Ala Glu Gly Gly Ser Ser Tyr Glu Asn Val

465 470 475 480

tcc tct ttg atc aga gat gtc act gaa act gtc cgt gct cct cat 1485

Ser Ser Leu Ile Arg Asp Val Thr Glu Thr Val Arg Ala Pro His

485 490 495

<210> 126

<211> 495

<212> PRT

<213> Stevia rebaudiana

<400> 126

Met Ser Pro Lys Met Val Ala Pro Pro Thr Asn Leu His Phe Val Leu

1 5 10 15

Phe Pro Leu Met Ala Gln Gly His Leu Val Pro Met Val Asp Ile Ala

20 25 30

Arg Ile Leu Ala Gln Arg Gly Ala Thr Val Thr Ile Ile Thr Thr Pro

35 40 45

Tyr His Ala Asn Arg Val Arg Pro Val Ile Ser Arg Ala Ile Ala Thr

50 55 60

Asn Leu Lys Ile Gln Leu Leu Glu Leu Gln Leu Arg Ser Thr Glu Ala

65 70 75 80

Gly Leu Pro Glu Gly Cys Glu Ser Phe Asp Gln Leu Pro Ser Phe Glu

85 90 95

Tyr Trp Lys Asn Ile Ser Thr Ala Ile Asp Leu Leu Gln Gln Pro Ala

100 105 110

Glu Asp Leu Leu Arg Glu Leu Ser Pro Pro Pro Asp Cys Ile Ile Ser

115 120 125

Asp Phe Leu Phe Pro Trp Thr Thr Asp Val Ala Arg Arg Leu Asn Ile

130 135 140

Pro Arg Leu Val Phe Asn Gly Pro Gly Cys Phe Tyr Leu Leu Cys Ile

145 150 155 160

His Val Ala Ile Thr Ser Asn Ile Leu Gly Glu Asn Glu Pro Val Ser

165 170 175

Ser Asn Thr Glu Arg Val Val Leu Pro Gly Leu Pro Asp Arg Ile Glu

180 185 190

Val Thr Lys Leu Gln Ile Val Gly Ser Ser Arg Pro Ala Asn Val Asp

195 200 205

Glu Met Gly Ser Trp Leu Arg Ala Val Glu Ala Glu Lys Ala Ser Phe

210 215 220

Gly Ile Val Val Asn Thr Phe Glu Glu Leu Glu Pro Glu Tyr Val Glu

225 230 235 240

Glu Tyr Lys Thr Val Lys Asp Lys Lys Met Trp Cys Ile Gly Pro Val

245 250 255

Ser Leu Cys Asn Lys Thr Gly Pro Asp Leu Ala Glu Arg Gly Asn Lys

260 265 270

Ala Ala Ile Thr Glu His Asn Cys Leu Lys Trp Leu Asp Glu Arg Lys

275 280 285

Leu Gly Ser Val Leu Tyr Val Cys Leu Gly Ser Leu Ala Arg Ile Ser

290 295 300

Ala Ala Gln Ala Ile Glu Leu Gly Leu Gly Leu Glu Ser Ile Asn Arg

305 310 315 320

Pro Phe Ile Trp Cys Val Arg Asn Glu Thr Asp Glu Leu Lys Thr Trp

325 330 335

Phe Leu Asp Gly Phe Glu Glu Arg Val Arg Asp Arg Gly Leu Ile Val

340 345 350

His Gly Trp Ala Pro Gln Val Leu Ile Leu Ser His Pro Thr Ile Gly

355 360 365

Gly Phe Leu Thr His Cys Gly Trp Asn Ser Thr Ile Glu Ser Ile Thr

370 375 380

Ala Gly Val Pro Met Ile Thr Trp Pro Phe Phe Ala Asp Gln Phe Leu

385 390 395 400

Asn Glu Ala Phe Ile Val Glu Val Leu Lys Ile Gly Val Arg Ile Gly

405 410 415

Val Glu Arg Ala Cys Leu Phe Gly Glu Glu Asp Lys Val Gly Val Leu

420 425 430

Val Lys Lys Glu Asp Val Lys Lys Ala Val Glu Cys Leu Met Asp Glu

435 440 445

Asp Glu Asp Gly Asp Gln Arg Arg Lys Arg Val Ile Glu Leu Ala Lys

450 455 460

Met Ala Lys Ile Ala Met Ala Glu Gly Gly Ser Ser Tyr Glu Asn Val

465 470 475 480

Ser Ser Leu Ile Arg Asp Val Thr Glu Thr Val Arg Ala Pro His

485 490 495

<210> 127

<211> 1272

<212> DNA

<213> Stevia rebaudiana

<220>

<221> CDS

<222> (1)..(1272)

<400> 127

atg ttg caa ttg gct acc tac ttg cac tct caa ggt atc tcc atc act 48

Met Leu Gln Leu Ala Thr Tyr Leu His Ser Gln Gly Ile Ser Ile Thr

1 5 10 15

att gct caa tac cca aac ttc aac tct cca gac tcc tcc aac cac cca 96

Ile Ala Gln Tyr Pro Asn Phe Asn Ser Pro Asp Ser Ser Asn His Pro

20 25 30

gaa ttg act ttc tta cca tta tct tcc ggt aac ttg tcc gtt gct gat 144

Glu Leu Thr Phe Leu Pro Leu Ser Ser Gly Asn Leu Ser Val Ala Asp

35 40 45

atc tct ggt ggt ttc ttc aaa ttc att caa acc ttg aac cac aac tgt 192

Ile Ser Gly Gly Phe Phe Lys Phe Ile Gln Thr Leu Asn His Asn Cys

50 55 60

aag cct cac ttc aga gaa tac ttg gtt caa aac atg tcc tct gat gac 240

Lys Pro His Phe Arg Glu Tyr Leu Val Gln Asn Met Ser Ser Asp Asp

65 70 75 80

aag gaa tcc att gtc atc atc aga gac aac ttg atg ttc ttt gct ggt 288

Lys Glu Ser Ile Val Ile Ile Arg Asp Asn Leu Met Phe Phe Ala Gly

85 90 95

gaa atc gct ggt gaa tta ggt tta cca tct atc atc ttg aga ggt tct 336

Glu Ile Ala Gly Glu Leu Gly Leu Pro Ser Ile Ile Leu Arg Gly Ser

100 105 110

aac gct gtc atg ttg act gct tcc gat atc att cca caa ttg cac caa 384

Asn Ala Val Met Leu Thr Ala Ser Asp Ile Ile Pro Gln Leu His Gln

115 120 125

gaa ggt aga ttc cca cca cca gac tct cta ttg caa gaa acc att cca 432

Glu Gly Arg Phe Pro Pro Pro Asp Ser Leu Leu Gln Glu Thr Ile Pro

130 135 140

gaa ttg gtt cca ttc aga tac aag gac ttg cca ttc atc ggt tac cca 480

Glu Leu Val Pro Phe Arg Tyr Lys Asp Leu Pro Phe Ile Gly Tyr Pro

145 150 155 160

att cac caa acc ttg gaa ttc tcc att acc atg atg acc cca aag tct 528

Ile His Gln Thr Leu Glu Phe Ser Ile Thr Met Met Thr Pro Lys Ser

165 170 175

cca gct tct gcc atc ttg atc aac act tta gaa ttc ttg gaa caa tct 576

Pro Ala Ser Ala Ile Leu Ile Asn Thr Leu Glu Phe Leu Glu Gln Ser

180 185 190

gct ttg act caa atc cgt gac cac tac aag gtc cca gtt ttc act atc 624

Ala Leu Thr Gln Ile Arg Asp His Tyr Lys Val Pro Val Phe Thr Ile

195 200 205

ggt cca ttg cac aag att gtc act acc aga tcc act tcc atc ttg gaa 672

Gly Pro Leu His Lys Ile Val Thr Thr Arg Ser Thr Ser Ile Leu Glu

210 215 220

gaa gac acc tct tgt atc aac tgg ttg gac aag caa tct cca aag tct 720

Glu Asp Thr Ser Cys Ile Asn Trp Leu Asp Lys Gln Ser Pro Lys Ser

225 230 235 240

gtt gtc tac gtt tct cta ggt tct ttg gcc aaa ttg gac gaa aag gtt 768

Val Val Tyr Val Ser Leu Gly Ser Leu Ala Lys Leu Asp Glu Lys Val

245 250 255

gct tct gaa atg gct tgt ggt ttg gcc atg tcc aac cat aaa ttc tta 816

Ala Ser Glu Met Ala Cys Gly Leu Ala Met Ser Asn His Lys Phe Leu

260 265 270

tgg gtt gtc aga cca ggt atg gtc cat ggt ttc gaa tgg gtt gaa ttt 864

Trp Val Val Arg Pro Gly Met Val His Gly Phe Glu Trp Val Glu Phe

275 280 285

ttg cca gac tct ttg gtt ggt gaa atg aag gcc aga ggt ttg att gtc 912

Leu Pro Asp Ser Leu Val Gly Glu Met Lys Ala Arg Gly Leu Ile Val

290 295 300

aaa tgg gct cca caa acc act gtt ttg gct cac aac gct gtc ggt ggt 960

Lys Trp Ala Pro Gln Thr Thr Val Leu Ala His Asn Ala Val Gly Gly

305 310 315 320

ttt tgg tcc cac tgt ggt tgg aac tct act atc gaa tgt ttg gct gaa 1008

Phe Trp Ser His Cys Gly Trp Asn Ser Thr Ile Glu Cys Leu Ala Glu

325 330 335

ggt gtt cca atg atg tgt caa cct ttc ttc gct gac caa ttg ttg aat 1056

Gly Val Pro Met Met Cys Gln Pro Phe Phe Ala Asp Gln Leu Leu Asn

340 345 350

gct cgt tac gtt tcc gat gtc tgg aag acc ggt ttc gaa att gtc att 1104

Ala Arg Tyr Val Ser Asp Val Trp Lys Thr Gly Phe Glu Ile Val Ile

355 360 365

gaa aag ggt gaa att gct tgt gcc atc aag aga gtt ttg gtc gat gaa 1152

Glu Lys Gly Glu Ile Ala Cys Ala Ile Lys Arg Val Leu Val Asp Glu

370 375 380

gaa ggt gaa gaa atg aga caa aga gct atg gaa atc aag gaa aag gtc 1200

Glu Gly Glu Glu Met Arg Gln Arg Ala Met Glu Ile Lys Glu Lys Val

385 390 395 400

aag att gcc atc aac gat ggt ggt tct tct tac gac tct ttc aag gat 1248

Lys Ile Ala Ile Asn Asp Gly Gly Ser Ser Tyr Asp Ser Phe Lys Asp

405 410 415

ttg gtt gct ttc atc tcc tct ttg 1272

Leu Val Ala Phe Ile Ser Ser Leu

420

<210> 128

<211> 424

<212> PRT

<213> Stevia rebaudiana

<400> 128

Met Leu Gln Leu Ala Thr Tyr Leu His Ser Gln Gly Ile Ser Ile Thr

1 5 10 15

Ile Ala Gln Tyr Pro Asn Phe Asn Ser Pro Asp Ser Ser Asn His Pro

20 25 30

Glu Leu Thr Phe Leu Pro Leu Ser Ser Gly Asn Leu Ser Val Ala Asp

35 40 45

Ile Ser Gly Gly Phe Phe Lys Phe Ile Gln Thr Leu Asn His Asn Cys

50 55 60

Lys Pro His Phe Arg Glu Tyr Leu Val Gln Asn Met Ser Ser Asp Asp

65 70 75 80

Lys Glu Ser Ile Val Ile Ile Arg Asp Asn Leu Met Phe Phe Ala Gly

85 90 95

Glu Ile Ala Gly Glu Leu Gly Leu Pro Ser Ile Ile Leu Arg Gly Ser

100 105 110

Asn Ala Val Met Leu Thr Ala Ser Asp Ile Ile Pro Gln Leu His Gln

115 120 125

Glu Gly Arg Phe Pro Pro Pro Asp Ser Leu Leu Gln Glu Thr Ile Pro

130 135 140

Glu Leu Val Pro Phe Arg Tyr Lys Asp Leu Pro Phe Ile Gly Tyr Pro

145 150 155 160

Ile His Gln Thr Leu Glu Phe Ser Ile Thr Met Met Thr Pro Lys Ser

165 170 175

Pro Ala Ser Ala Ile Leu Ile Asn Thr Leu Glu Phe Leu Glu Gln Ser

180 185 190

Ala Leu Thr Gln Ile Arg Asp His Tyr Lys Val Pro Val Phe Thr Ile

195 200 205

Gly Pro Leu His Lys Ile Val Thr Thr Arg Ser Thr Ser Ile Leu Glu

210 215 220

Glu Asp Thr Ser Cys Ile Asn Trp Leu Asp Lys Gln Ser Pro Lys Ser

225 230 235 240

Val Val Tyr Val Ser Leu Gly Ser Leu Ala Lys Leu Asp Glu Lys Val

245 250 255

Ala Ser Glu Met Ala Cys Gly Leu Ala Met Ser Asn His Lys Phe Leu

260 265 270

Trp Val Val Arg Pro Gly Met Val His Gly Phe Glu Trp Val Glu Phe

275 280 285

Leu Pro Asp Ser Leu Val Gly Glu Met Lys Ala Arg Gly Leu Ile Val

290 295 300

Lys Trp Ala Pro Gln Thr Thr Val Leu Ala His Asn Ala Val Gly Gly

305 310 315 320

Phe Trp Ser His Cys Gly Trp Asn Ser Thr Ile Glu Cys Leu Ala Glu

325 330 335

Gly Val Pro Met Met Cys Gln Pro Phe Phe Ala Asp Gln Leu Leu Asn

340 345 350

Ala Arg Tyr Val Ser Asp Val Trp Lys Thr Gly Phe Glu Ile Val Ile

355 360 365

Glu Lys Gly Glu Ile Ala Cys Ala Ile Lys Arg Val Leu Val Asp Glu

370 375 380

Glu Gly Glu Glu Met Arg Gln Arg Ala Met Glu Ile Lys Glu Lys Val

385 390 395 400

Lys Ile Ala Ile Asn Asp Gly Gly Ser Ser Tyr Asp Ser Phe Lys Asp

405 410 415

Leu Val Ala Phe Ile Ser Ser Leu

420

<210> 129

<211> 1422

<212> DNA

<213> Stevia rebaudiana

<220>

<221> CDS

<222> (1)..(1422)

<400> 129

atg tct acc tct gaa ttg gtt ttc att cca tct cca ggt gct ggt cat 48

Met Ser Thr Ser Glu Leu Val Phe Ile Pro Ser Pro Gly Ala Gly His

1 5 10 15

ttg cca cca act gtt gaa ttg gcc aaa ttg ttg ttg cac cgt gac caa 96

Leu Pro Pro Thr Val Glu Leu Ala Lys Leu Leu Leu His Arg Asp Gln

20 25 30

aga ttg tct gtc acc atc att gtc atg aac tta tgg tta ggt cca aag 144

Arg Leu Ser Val Thr Ile Ile Val Met Asn Leu Trp Leu Gly Pro Lys

35 40 45

cac aac act gaa gcc aga cca tgt gtt cct tcc ttg aga ttc gtc gat 192

His Asn Thr Glu Ala Arg Pro Cys Val Pro Ser Leu Arg Phe Val Asp

50 55 60

atc cca tgt gac gaa tct acc atg gct ttg atc tct cca aac act ttc 240

Ile Pro Cys Asp Glu Ser Thr Met Ala Leu Ile Ser Pro Asn Thr Phe

65 70 75 80

atc tct gcc ttt gtc gaa cac cac aag cca aga gtt cgt gac atc gtt 288

Ile Ser Ala Phe Val Glu His His Lys Pro Arg Val Arg Asp Ile Val

85 90 95

cgt ggt atc att gaa tct gac tct gtc aga ttg gct ggt ttc gtc ttg 336

Arg Gly Ile Ile Glu Ser Asp Ser Val Arg Leu Ala Gly Phe Val Leu

100 105 110

gac atg ttc tgt atg cca atg tcc gat gtt gct aac gaa ttt ggt gtt 384

Asp Met Phe Cys Met Pro Met Ser Asp Val Ala Asn Glu Phe Gly Val

115 120 125

cca tct tac aac tac ttc acc tct ggt gct gcc act tta ggt ttg atg 432

Pro Ser Tyr Asn Tyr Phe Thr Ser Gly Ala Ala Thr Leu Gly Leu Met

130 135 140

ttc cat ttg caa tgg aag aga gat cac gaa ggt tac gac gct act gaa 480

Phe His Leu Gln Trp Lys Arg Asp His Glu Gly Tyr Asp Ala Thr Glu

145 150 155 160

ttg aag aac tct gac act gaa tta tct gtc cca tct tac gtt aac cct 528

Leu Lys Asn Ser Asp Thr Glu Leu Ser Val Pro Ser Tyr Val Asn Pro

165 170 175

gtt cca gcc aag gtc ttg cca gaa gtt gtt ttg gac aag gaa ggt ggt 576

Val Pro Ala Lys Val Leu Pro Glu Val Val Leu Asp Lys Glu Gly Gly

180 185 190

tcc aag atg ttc ttg gat ttg gct gaa aga atc aga gaa tcc aag ggt 624

Ser Lys Met Phe Leu Asp Leu Ala Glu Arg Ile Arg Glu Ser Lys Gly

195 200 205

atc atc gtt aac tct tgt caa gct att gaa aga cac gct ttg gaa tac 672

Ile Ile Val Asn Ser Cys Gln Ala Ile Glu Arg His Ala Leu Glu Tyr

210 215 220

cta tcc tcc aac aac aac ggt att cca cct gtt ttc cca gtc ggt cca 720

Leu Ser Ser Asn Asn Asn Gly Ile Pro Pro Val Phe Pro Val Gly Pro

225 230 235 240

atc ttg aac ttg gaa aac aag aag gac gat gct aag act gac gaa atc 768

Ile Leu Asn Leu Glu Asn Lys Lys Asp Asp Ala Lys Thr Asp Glu Ile

245 250 255

atg aga tgg ttg aac gaa caa cca gaa tcc tct gtt gtt ttc ttg tgt 816

Met Arg Trp Leu Asn Glu Gln Pro Glu Ser Ser Val Val Phe Leu Cys

260 265 270

ttc ggt tcc atg ggt tct ttc aac gaa aag caa gtc aaa gaa att gct 864

Phe Gly Ser Met Gly Ser Phe Asn Glu Lys Gln Val Lys Glu Ile Ala

275 280 285

gtt gcc att gaa aga tct ggt cac aga ttt tta tgg tct ttg aga aga 912

Val Ala Ile Glu Arg Ser Gly His Arg Phe Leu Trp Ser Leu Arg Arg

290 295 300

cca act cca aag gaa aag att gaa ttc cca aag gaa tac gaa aac ttg 960

Pro Thr Pro Lys Glu Lys Ile Glu Phe Pro Lys Glu Tyr Glu Asn Leu

305 310 315 320

gaa gaa gtc tta cca gaa ggt ttc ttg aag aga acc tct tct atc ggt 1008

Glu Glu Val Leu Pro Glu Gly Phe Leu Lys Arg Thr Ser Ser Ile Gly

325 330 335

aag gtt atc ggt tgg gct cca caa atg gct gtc ttg tcc cac cca tcc 1056

Lys Val Ile Gly Trp Ala Pro Gln Met Ala Val Leu Ser His Pro Ser

340 345 350

gtc ggt ggt ttc gtt tcc cac tgt ggt tgg aac tcc act ttg gaa tcc 1104

Val Gly Gly Phe Val Ser His Cys Gly Trp Asn Ser Thr Leu Glu Ser

355 360 365

atg tgg tgt ggt gtc cca atg gct gct tgg cca tta tac gct gaa caa 1152

Met Trp Cys Gly Val Pro Met Ala Ala Trp Pro Leu Tyr Ala Glu Gln

370 375 380

act ttg aat gct ttc ttg ttg gtt gtc gaa ttg ggt ttg gct gct gaa 1200

Thr Leu Asn Ala Phe Leu Leu Val Val Glu Leu Gly Leu Ala Ala Glu

385 390 395 400

att aga atg gac tac aga acc gat acc aag gct ggt tac gat ggt ggt 1248

Ile Arg Met Asp Tyr Arg Thr Asp Thr Lys Ala Gly Tyr Asp Gly Gly

405 410 415

atg gaa gtc acc gtt gaa gaa att gaa gac ggt atc aga aag ttg atg 1296

Met Glu Val Thr Val Glu Glu Ile Glu Asp Gly Ile Arg Lys Leu Met

420 425 430

tct gac ggt gaa atc aga aac aag gtc aag gat gtc aag gaa aaa tcc 1344

Ser Asp Gly Glu Ile Arg Asn Lys Val Lys Asp Val Lys Glu Lys Ser

435 440 445

aga gct gcc gtt gtt gag ggt ggt tct tct tac gct tcc att ggt aaa 1392

Arg Ala Ala Val Val Glu Gly Gly Ser Ser Tyr Ala Ser Ile Gly Lys

450 455 460

ttc atc gaa cac gtt tcc aac gtc acc ata 1422

Phe Ile Glu His Val Ser Asn Val Thr Ile

465 470

<210> 130

<211> 474

<212> PRT

<213> Stevia rebaudiana

<400> 130

Met Ser Thr Ser Glu Leu Val Phe Ile Pro Ser Pro Gly Ala Gly His

1 5 10 15

Leu Pro Pro Thr Val Glu Leu Ala Lys Leu Leu Leu His Arg Asp Gln

20 25 30

Arg Leu Ser Val Thr Ile Ile Val Met Asn Leu Trp Leu Gly Pro Lys

35 40 45

His Asn Thr Glu Ala Arg Pro Cys Val Pro Ser Leu Arg Phe Val Asp

50 55 60

Ile Pro Cys Asp Glu Ser Thr Met Ala Leu Ile Ser Pro Asn Thr Phe

65 70 75 80

Ile Ser Ala Phe Val Glu His His Lys Pro Arg Val Arg Asp Ile Val

85 90 95

Arg Gly Ile Ile Glu Ser Asp Ser Val Arg Leu Ala Gly Phe Val Leu

100 105 110

Asp Met Phe Cys Met Pro Met Ser Asp Val Ala Asn Glu Phe Gly Val

115 120 125

Pro Ser Tyr Asn Tyr Phe Thr Ser Gly Ala Ala Thr Leu Gly Leu Met

130 135 140

Phe His Leu Gln Trp Lys Arg Asp His Glu Gly Tyr Asp Ala Thr Glu

145 150 155 160

Leu Lys Asn Ser Asp Thr Glu Leu Ser Val Pro Ser Tyr Val Asn Pro

165 170 175

Val Pro Ala Lys Val Leu Pro Glu Val Val Leu Asp Lys Glu Gly Gly

180 185 190

Ser Lys Met Phe Leu Asp Leu Ala Glu Arg Ile Arg Glu Ser Lys Gly

195 200 205

Ile Ile Val Asn Ser Cys Gln Ala Ile Glu Arg His Ala Leu Glu Tyr

210 215 220

Leu Ser Ser Asn Asn Asn Gly Ile Pro Pro Val Phe Pro Val Gly Pro

225 230 235 240

Ile Leu Asn Leu Glu Asn Lys Lys Asp Asp Ala Lys Thr Asp Glu Ile

245 250 255

Met Arg Trp Leu Asn Glu Gln Pro Glu Ser Ser Val Val Phe Leu Cys

260 265 270

Phe Gly Ser Met Gly Ser Phe Asn Glu Lys Gln Val Lys Glu Ile Ala

275 280 285

Val Ala Ile Glu Arg Ser Gly His Arg Phe Leu Trp Ser Leu Arg Arg

290 295 300

Pro Thr Pro Lys Glu Lys Ile Glu Phe Pro Lys Glu Tyr Glu Asn Leu

305 310 315 320

Glu Glu Val Leu Pro Glu Gly Phe Leu Lys Arg Thr Ser Ser Ile Gly

325 330 335

Lys Val Ile Gly Trp Ala Pro Gln Met Ala Val Leu Ser His Pro Ser

340 345 350

Val Gly Gly Phe Val Ser His Cys Gly Trp Asn Ser Thr Leu Glu Ser

355 360 365

Met Trp Cys Gly Val Pro Met Ala Ala Trp Pro Leu Tyr Ala Glu Gln

370 375 380

Thr Leu Asn Ala Phe Leu Leu Val Val Glu Leu Gly Leu Ala Ala Glu

385 390 395 400

Ile Arg Met Asp Tyr Arg Thr Asp Thr Lys Ala Gly Tyr Asp Gly Gly

405 410 415

Met Glu Val Thr Val Glu Glu Ile Glu Asp Gly Ile Arg Lys Leu Met

420 425 430

Ser Asp Gly Glu Ile Arg Asn Lys Val Lys Asp Val Lys Glu Lys Ser

435 440 445

Arg Ala Ala Val Val Glu Gly Gly Ser Ser Tyr Ala Ser Ile Gly Lys

450 455 460

Phe Ile Glu His Val Ser Asn Val Thr Ile

465 470

<210> 131

<211> 1437

<212> DNA

<213> Stevia rebaudiana

<220>

<221> CDS

<222> (1)..(1437)

<400> 131

atg gcc tcc att gct gaa atg caa aag cct cat gcc atc tgt atc cca 48

Met Ala Ser Ile Ala Glu Met Gln Lys Pro His Ala Ile Cys Ile Pro

1 5 10 15

tac cca gct caa ggt cac atc aac cca atg atg caa ttt gcc aaa ttg 96

Tyr Pro Ala Gln Gly His Ile Asn Pro Met Met Gln Phe Ala Lys Leu

20 25 30

ttg cac ttc aag ggt ttc cac atc tct ttc gtt aac aac cat tac aac 144

Leu His Phe Lys Gly Phe His Ile Ser Phe Val Asn Asn His Tyr Asn

35 40 45

cac aag aga tta caa aga tct aga ggt cta tcc gct ttg gaa ggt ttg 192

His Lys Arg Leu Gln Arg Ser Arg Gly Leu Ser Ala Leu Glu Gly Leu

50 55 60

cca gac ttc cac ttc tac tcc atc cca gat ggt ttg cct cca tct aac 240

Pro Asp Phe His Phe Tyr Ser Ile Pro Asp Gly Leu Pro Pro Ser Asn

65 70 75 80

gct gaa gct act caa tcc att cca ggt cta tgt gaa tct att cca aag 288

Ala Glu Ala Thr Gln Ser Ile Pro Gly Leu Cys Glu Ser Ile Pro Lys

85 90 95

cac tct ttg gaa cca ttc tgt gac ttg att gcc act ttg aat ggt tct 336

His Ser Leu Glu Pro Phe Cys Asp Leu Ile Ala Thr Leu Asn Gly Ser

100 105 110

gat gtt cca cca gtc tcc tgt atc atc tct gac ggt gtc atg tct ttc 384

Asp Val Pro Pro Val Ser Cys Ile Ile Ser Asp Gly Val Met Ser Phe

115 120 125

act ttg caa gct gct gaa aga ttc ggt tta cca gaa gtc tta ttc tgg 432

Thr Leu Gln Ala Ala Glu Arg Phe Gly Leu Pro Glu Val Leu Phe Trp

130 135 140

act cca tct gct tgt ggt ttc ttg gct tac act cac tac aga gat ttg 480

Thr Pro Ser Ala Cys Gly Phe Leu Ala Tyr Thr His Tyr Arg Asp Leu

145 150 155 160

gtt gac aaa gaa tac att cca ttg aag gac acc aat gat ttg acc aac 528

Val Asp Lys Glu Tyr Ile Pro Leu Lys Asp Thr Asn Asp Leu Thr Asn

165 170 175

ggt tac ttg gaa act tct ttg gac tgg atc cca ggt atg aag aac atc 576

Gly Tyr Leu Glu Thr Ser Leu Asp Trp Ile Pro Gly Met Lys Asn Ile

180 185 190

aga tta aag gac ttc cca tct ttc atc aga acc acc gat atc aac gat 624

Arg Leu Lys Asp Phe Pro Ser Phe Ile Arg Thr Thr Asp Ile Asn Asp

195 200 205

atc atg ttg aac tac ttc ttg att gaa act gaa gcc att cca aag ggt 672

Ile Met Leu Asn Tyr Phe Leu Ile Glu Thr Glu Ala Ile Pro Lys Gly

210 215 220

gtt gcc atc atc ttg aac act ttc gat gct ttg gaa aag gac tct atc 720

Val Ala Ile Ile Leu Asn Thr Phe Asp Ala Leu Glu Lys Asp Ser Ile

225 230 235 240

act cca gtc ttg gct ttg aac cct caa atc tac acc atc ggt cca ttg 768

Thr Pro Val Leu Ala Leu Asn Pro Gln Ile Tyr Thr Ile Gly Pro Leu

245 250 255

cac atg atg caa caa tac gtc gac cat gat gaa aga ttg aag cac att 816

His Met Met Gln Gln Tyr Val Asp His Asp Glu Arg Leu Lys His Ile

260 265 270

ggt tcc aac tta tgg aag gaa gac gtt tct tgt atc aac tgg ttg gac 864

Gly Ser Asn Leu Trp Lys Glu Asp Val Ser Cys Ile Asn Trp Leu Asp

275 280 285

acc aag aag cca aac tcc gtt gtc tac gtc aac ttt ggt tcc atc act 912

Thr Lys Lys Pro Asn Ser Val Val Tyr Val Asn Phe Gly Ser Ile Thr

290 295 300

gtt atg acc aag gaa caa ttg att gaa ttc ggt tgg ggt ttg gct aac 960

Val Met Thr Lys Glu Gln Leu Ile Glu Phe Gly Trp Gly Leu Ala Asn

305 310 315 320

tct aag aag gac ttc tta tgg atc acc aga cca gac att gtt ggt ggt 1008

Ser Lys Lys Asp Phe Leu Trp Ile Thr Arg Pro Asp Ile Val Gly Gly

325 330 335

aac gaa gct atg att cca gct gaa ttc att gaa gaa acc aag gaa aga 1056

Asn Glu Ala Met Ile Pro Ala Glu Phe Ile Glu Glu Thr Lys Glu Arg

340 345 350

ggt atg gtt act tcc tgg tgt tcc caa gaa gaa gtt ttg aag cac cca 1104

Gly Met Val Thr Ser Trp Cys Ser Gln Glu Glu Val Leu Lys His Pro

355 360 365

tcc att ggt gtt ttc ttg acc cac tct ggt tgg aac tcc acc att gaa 1152

Ser Ile Gly Val Phe Leu Thr His Ser Gly Trp Asn Ser Thr Ile Glu

370 375 380

tcc atc tct aac ggt gtt cca atg atc tgt tgg cca ttc ttc gct gaa 1200

Ser Ile Ser Asn Gly Val Pro Met Ile Cys Trp Pro Phe Phe Ala Glu

385 390 395 400

caa caa acc aac tgt cgt tac tgt tgt gtc gaa tgg gaa atc ggt ttg 1248

Gln Gln Thr Asn Cys Arg Tyr Cys Cys Val Glu Trp Glu Ile Gly Leu

405 410 415

gaa att gac act gac gtc aag cgt gaa gaa gtt gaa gct caa gtt aga 1296

Glu Ile Asp Thr Asp Val Lys Arg Glu Glu Val Glu Ala Gln Val Arg

420 425 430

gaa atg atg gat ggt tct aag ggt aag atg atg aaa aac aag gct ttg 1344

Glu Met Met Asp Gly Ser Lys Gly Lys Met Met Lys Asn Lys Ala Leu

435 440 445

gaa tgg aag aag aag gct gaa gaa gct gtc tct att ggt ggt tct tct 1392

Glu Trp Lys Lys Lys Ala Glu Glu Ala Val Ser Ile Gly Gly Ser Ser

450 455 460

tac ttg aac ttt gaa aaa ttg gtc acc gat gtc ttg ttg cgc aaa 1437

Tyr Leu Asn Phe Glu Lys Leu Val Thr Asp Val Leu Leu Arg Lys

465 470 475

<210> 132

<211> 479

<212> PRT

<213> Stevia rebaudiana

<400> 132

Met Ala Ser Ile Ala Glu Met Gln Lys Pro His Ala Ile Cys Ile Pro

1 5 10 15

Tyr Pro Ala Gln Gly His Ile Asn Pro Met Met Gln Phe Ala Lys Leu

20 25 30

Leu His Phe Lys Gly Phe His Ile Ser Phe Val Asn Asn His Tyr Asn

35 40 45

His Lys Arg Leu Gln Arg Ser Arg Gly Leu Ser Ala Leu Glu Gly Leu

50 55 60

Pro Asp Phe His Phe Tyr Ser Ile Pro Asp Gly Leu Pro Pro Ser Asn

65 70 75 80

Ala Glu Ala Thr Gln Ser Ile Pro Gly Leu Cys Glu Ser Ile Pro Lys

85 90 95

His Ser Leu Glu Pro Phe Cys Asp Leu Ile Ala Thr Leu Asn Gly Ser

100 105 110

Asp Val Pro Pro Val Ser Cys Ile Ile Ser Asp Gly Val Met Ser Phe

115 120 125

Thr Leu Gln Ala Ala Glu Arg Phe Gly Leu Pro Glu Val Leu Phe Trp

130 135 140

Thr Pro Ser Ala Cys Gly Phe Leu Ala Tyr Thr His Tyr Arg Asp Leu

145 150 155 160

Val Asp Lys Glu Tyr Ile Pro Leu Lys Asp Thr Asn Asp Leu Thr Asn

165 170 175

Gly Tyr Leu Glu Thr Ser Leu Asp Trp Ile Pro Gly Met Lys Asn Ile

180 185 190

Arg Leu Lys Asp Phe Pro Ser Phe Ile Arg Thr Thr Asp Ile Asn Asp

195 200 205

Ile Met Leu Asn Tyr Phe Leu Ile Glu Thr Glu Ala Ile Pro Lys Gly

210 215 220

Val Ala Ile Ile Leu Asn Thr Phe Asp Ala Leu Glu Lys Asp Ser Ile

225 230 235 240

Thr Pro Val Leu Ala Leu Asn Pro Gln Ile Tyr Thr Ile Gly Pro Leu

245 250 255

His Met Met Gln Gln Tyr Val Asp His Asp Glu Arg Leu Lys His Ile

260 265 270

Gly Ser Asn Leu Trp Lys Glu Asp Val Ser Cys Ile Asn Trp Leu Asp

275 280 285

Thr Lys Lys Pro Asn Ser Val Val Tyr Val Asn Phe Gly Ser Ile Thr

290 295 300

Val Met Thr Lys Glu Gln Leu Ile Glu Phe Gly Trp Gly Leu Ala Asn

305 310 315 320

Ser Lys Lys Asp Phe Leu Trp Ile Thr Arg Pro Asp Ile Val Gly Gly

325 330 335

Asn Glu Ala Met Ile Pro Ala Glu Phe Ile Glu Glu Thr Lys Glu Arg

340 345 350

Gly Met Val Thr Ser Trp Cys Ser Gln Glu Glu Val Leu Lys His Pro

355 360 365

Ser Ile Gly Val Phe Leu Thr His Ser Gly Trp Asn Ser Thr Ile Glu

370 375 380

Ser Ile Ser Asn Gly Val Pro Met Ile Cys Trp Pro Phe Phe Ala Glu

385 390 395 400

Gln Gln Thr Asn Cys Arg Tyr Cys Cys Val Glu Trp Glu Ile Gly Leu

405 410 415

Glu Ile Asp Thr Asp Val Lys Arg Glu Glu Val Glu Ala Gln Val Arg

420 425 430

Glu Met Met Asp Gly Ser Lys Gly Lys Met Met Lys Asn Lys Ala Leu

435 440 445

Glu Trp Lys Lys Lys Ala Glu Glu Ala Val Ser Ile Gly Gly Ser Ser

450 455 460

Tyr Leu Asn Phe Glu Lys Leu Val Thr Asp Val Leu Leu Arg Lys

465 470 475

<210> 133

<211> 1374

<212> DNA

<213> Stevia rebaudiana

<220>

<221> CDS

<222> (1)..(1374)

<400> 133

atg gaa aac aag act gaa act acc gtt cgt cgt aga aga aga atc atc 48

Met Glu Asn Lys Thr Glu Thr Thr Val Arg Arg Arg Arg Arg Ile Ile

1 5 10 15

tta ttc cca gtt cca gtt caa ggt cac atc aac cca att ttg caa ttg 96

Leu Phe Pro Val Pro Val Gln Gly His Ile Asn Pro Ile Leu Gln Leu

20 25 30

gct aac gtc tta tac tcc aag ggt ttc tcc atc act att ttc cac act 144

Ala Asn Val Leu Tyr Ser Lys Gly Phe Ser Ile Thr Ile Phe His Thr

35 40 45

aac ttc aac aag cca aag acc tcc aac tac cca cac ttc acc ttc aga 192

Asn Phe Asn Lys Pro Lys Thr Ser Asn Tyr Pro His Phe Thr Phe Arg

50 55 60

ttt att ttg gac aat gac cct caa gat gtc aga atc tcc aac ttg cca 240

Phe Ile Leu Asp Asn Asp Pro Gln Asp Val Arg Ile Ser Asn Leu Pro

65 70 75 80

act cac ggt cca ttg acc gtc atg aga att ttg atc atc aac gaa cac 288

Thr His Gly Pro Leu Thr Val Met Arg Ile Leu Ile Ile Asn Glu His

85 90 95

ggt gct gat gaa ttg caa aga gaa ttg gaa ttg ttg atg ttg gcc tcc 336

Gly Ala Asp Glu Leu Gln Arg Glu Leu Glu Leu Leu Met Leu Ala Ser

100 105 110

gaa gaa gac ggt gaa gtc tcc tgt ttg att acc gac caa atc tgg tac 384

Glu Glu Asp Gly Glu Val Ser Cys Leu Ile Thr Asp Gln Ile Trp Tyr

115 120 125

ttc acc caa tct gtt gct gac tct ttg aac ttg aga aga ttg gtt ttg 432

Phe Thr Gln Ser Val Ala Asp Ser Leu Asn Leu Arg Arg Leu Val Leu

130 135 140

atg act tct tct ttg ttc aac ttc cac gct cat gtt tct ttg cct caa 480

Met Thr Ser Ser Leu Phe Asn Phe His Ala His Val Ser Leu Pro Gln

145 150 155 160

ttc gat gaa ttg ggt tac ttg gac cca gac gac aag act aga tta gaa 528

Phe Asp Glu Leu Gly Tyr Leu Asp Pro Asp Asp Lys Thr Arg Leu Glu

165 170 175

gaa caa gct tct ggt ttc cca atg ttg aag gtc aag gat atc aaa tgt 576

Glu Gln Ala Ser Gly Phe Pro Met Leu Lys Val Lys Asp Ile Lys Cys

180 185 190

ggt ttc tct atg tgg aag caa ggt aag gaa atc ttt gaa aac atc acc 624

Gly Phe Ser Met Trp Lys Gln Gly Lys Glu Ile Phe Glu Asn Ile Thr

195 200 205

aag caa acc aag gct tct tct ggt gtc atc tgg aac tct ttc aag gaa 672

Lys Gln Thr Lys Ala Ser Ser Gly Val Ile Trp Asn Ser Phe Lys Glu

210 215 220

ttg gaa gaa tct gaa ttg gaa acc gtc atc aga gaa atc cca gct cca 720

Leu Glu Glu Ser Glu Leu Glu Thr Val Ile Arg Glu Ile Pro Ala Pro

225 230 235 240

tcc ttc ttg att cca ttg cca aag cat ttg act gct tcc tct tct tct 768

Ser Phe Leu Ile Pro Leu Pro Lys His Leu Thr Ala Ser Ser Ser Ser

245 250 255

cta tta gac cac gac aga act gtt ttc cca tgg tta gac caa caa cca 816

Leu Leu Asp His Asp Arg Thr Val Phe Pro Trp Leu Asp Gln Gln Pro

260 265 270

tct cgt tct gtc ttg tac gtt tct ttc ggt tct gct act gaa gtc gat 864

Ser Arg Ser Val Leu Tyr Val Ser Phe Gly Ser Ala Thr Glu Val Asp

275 280 285

gct aag gac ttc ttg gaa att gct cgt ggt ttg gtc gac tcc aag caa 912

Ala Lys Asp Phe Leu Glu Ile Ala Arg Gly Leu Val Asp Ser Lys Gln

290 295 300

tct ttc tta tgg gtt gtt aga cca ggt ttc gtc aag ggt tcc acc tgg 960

Ser Phe Leu Trp Val Val Arg Pro Gly Phe Val Lys Gly Ser Thr Trp

305 310 315 320

gtt gaa cca ttg cca gat ggt ttc ttg ggt gaa aga ggt aga att gtc 1008

Val Glu Pro Leu Pro Asp Gly Phe Leu Gly Glu Arg Gly Arg Ile Val

325 330 335

aaa tgg gtt cca caa caa gaa gtt ttg gct cac ggt gcc att ggt gct 1056

Lys Trp Val Pro Gln Gln Glu Val Leu Ala His Gly Ala Ile Gly Ala

340 345 350

ttc tgg acc cac tct ggt tgg aac tcc act ttg gaa tcc gtt tgt gaa 1104

Phe Trp Thr His Ser Gly Trp Asn Ser Thr Leu Glu Ser Val Cys Glu

355 360 365

ggt gtt cca atg att ttc tct gcc ttt gcc ttt gac caa cca tta aac 1152

Gly Val Pro Met Ile Phe Ser Ala Phe Ala Phe Asp Gln Pro Leu Asn

370 375 380

gct aga tac atg tcc gat gtc ttg aag gtt ggt gtc tac ttg gaa aac 1200

Ala Arg Tyr Met Ser Asp Val Leu Lys Val Gly Val Tyr Leu Glu Asn

385 390 395 400

ggt tgg gaa aga ggt gaa att gcc aac gcc atc aga aga gtt atg gtc 1248

Gly Trp Glu Arg Gly Glu Ile Ala Asn Ala Ile Arg Arg Val Met Val

405 410 415

gat gaa gaa ggt ggt tac atc aga caa aac gct tcc gtc ttg aag caa 1296

Asp Glu Glu Gly Gly Tyr Ile Arg Gln Asn Ala Ser Val Leu Lys Gln

420 425 430

aag gct gat gtt tcc ttg atg aag ggt ggt tct tct tac gaa tct cta 1344

Lys Ala Asp Val Ser Leu Met Lys Gly Gly Ser Ser Tyr Glu Ser Leu

435 440 445

gaa tct ttg gtt gct tac atc tcc agc tta 1374

Glu Ser Leu Val Ala Tyr Ile Ser Ser Leu

450 455

<210> 134

<211> 458

<212> PRT

<213> Stevia rebaudiana

<400> 134

Met Glu Asn Lys Thr Glu Thr Thr Val Arg Arg Arg Arg Arg Ile Ile

1 5 10 15

Leu Phe Pro Val Pro Val Gln Gly His Ile Asn Pro Ile Leu Gln Leu

20 25 30

Ala Asn Val Leu Tyr Ser Lys Gly Phe Ser Ile Thr Ile Phe His Thr

35 40 45

Asn Phe Asn Lys Pro Lys Thr Ser Asn Tyr Pro His Phe Thr Phe Arg

50 55 60

Phe Ile Leu Asp Asn Asp Pro Gln Asp Val Arg Ile Ser Asn Leu Pro

65 70 75 80

Thr His Gly Pro Leu Thr Val Met Arg Ile Leu Ile Ile Asn Glu His

85 90 95

Gly Ala Asp Glu Leu Gln Arg Glu Leu Glu Leu Leu Met Leu Ala Ser

100 105 110

Glu Glu Asp Gly Glu Val Ser Cys Leu Ile Thr Asp Gln Ile Trp Tyr

115 120 125

Phe Thr Gln Ser Val Ala Asp Ser Leu Asn Leu Arg Arg Leu Val Leu

130 135 140

Met Thr Ser Ser Leu Phe Asn Phe His Ala His Val Ser Leu Pro Gln

145 150 155 160

Phe Asp Glu Leu Gly Tyr Leu Asp Pro Asp Asp Lys Thr Arg Leu Glu

165 170 175

Glu Gln Ala Ser Gly Phe Pro Met Leu Lys Val Lys Asp Ile Lys Cys

180 185 190

Gly Phe Ser Met Trp Lys Gln Gly Lys Glu Ile Phe Glu Asn Ile Thr

195 200 205

Lys Gln Thr Lys Ala Ser Ser Gly Val Ile Trp Asn Ser Phe Lys Glu

210 215 220

Leu Glu Glu Ser Glu Leu Glu Thr Val Ile Arg Glu Ile Pro Ala Pro

225 230 235 240

Ser Phe Leu Ile Pro Leu Pro Lys His Leu Thr Ala Ser Ser Ser Ser

245 250 255

Leu Leu Asp His Asp Arg Thr Val Phe Pro Trp Leu Asp Gln Gln Pro

260 265 270

Ser Arg Ser Val Leu Tyr Val Ser Phe Gly Ser Ala Thr Glu Val Asp

275 280 285

Ala Lys Asp Phe Leu Glu Ile Ala Arg Gly Leu Val Asp Ser Lys Gln

290 295 300

Ser Phe Leu Trp Val Val Arg Pro Gly Phe Val Lys Gly Ser Thr Trp

305 310 315 320

Val Glu Pro Leu Pro Asp Gly Phe Leu Gly Glu Arg Gly Arg Ile Val

325 330 335

Lys Trp Val Pro Gln Gln Glu Val Leu Ala His Gly Ala Ile Gly Ala

340 345 350

Phe Trp Thr His Ser Gly Trp Asn Ser Thr Leu Glu Ser Val Cys Glu

355 360 365

Gly Val Pro Met Ile Phe Ser Ala Phe Ala Phe Asp Gln Pro Leu Asn

370 375 380

Ala Arg Tyr Met Ser Asp Val Leu Lys Val Gly Val Tyr Leu Glu Asn

385 390 395 400

Gly Trp Glu Arg Gly Glu Ile Ala Asn Ala Ile Arg Arg Val Met Val

405 410 415

Asp Glu Glu Gly Gly Tyr Ile Arg Gln Asn Ala Ser Val Leu Lys Gln

420 425 430

Lys Ala Asp Val Ser Leu Met Lys Gly Gly Ser Ser Tyr Glu Ser Leu

435 440 445

Glu Ser Leu Val Ala Tyr Ile Ser Ser Leu

450 455

<210> 135

<211> 1377

<212> DNA

<213> Stevia rebaudiana

<220>

<221> CDS

<222> (1)..(1377)

<400> 135

atg gaa aac aag act gaa act act gtc aga aga aga cgt cgt atc atc 48

Met Glu Asn Lys Thr Glu Thr Thr Val Arg Arg Arg Arg Arg Ile Ile

1 5 10 15

ttg ttc cca gtt cca ttc caa ggt cac atc aac cca atg tta caa tta 96

Leu Phe Pro Val Pro Phe Gln Gly His Ile Asn Pro Met Leu Gln Leu

20 25 30

gct aac gtc ttg tac tcc aag ggt ttc tcc atc acc att ttc cac acc 144

Ala Asn Val Leu Tyr Ser Lys Gly Phe Ser Ile Thr Ile Phe His Thr

35 40 45

aac ttc aac aag cca aag act tct aac tac cca cac ttc act ttc aga 192

Asn Phe Asn Lys Pro Lys Thr Ser Asn Tyr Pro His Phe Thr Phe Arg

50 55 60

ttt atc ttg gac aac gac cct caa gat gtc aga att tcc aac ttg cca 240

Phe Ile Leu Asp Asn Asp Pro Gln Asp Val Arg Ile Ser Asn Leu Pro

65 70 75 80

acc cac ggt cca ttg gct gtc atg aga att ttg atc atc aac gaa cac 288

Thr His Gly Pro Leu Ala Val Met Arg Ile Leu Ile Ile Asn Glu His

85 90 95

ggt gcc gac gaa ttg aga aga gaa ttg gaa ttg ttg atg ttg gct tct 336

Gly Ala Asp Glu Leu Arg Arg Glu Leu Glu Leu Leu Met Leu Ala Ser

100 105 110

gaa gaa gat ggt gaa gtt tcc tgt ttg att gct gac caa atc tgg tac 384

Glu Glu Asp Gly Glu Val Ser Cys Leu Ile Ala Asp Gln Ile Trp Tyr

115 120 125

ttc acc caa tct gtt gct gac tct ttg aac ttg aga aga ttg gtc ttg 432

Phe Thr Gln Ser Val Ala Asp Ser Leu Asn Leu Arg Arg Leu Val Leu

130 135 140

gtc act tct tct ttg ttc aac ttc cac gct cat gtc tct ttg cca caa 480

Val Thr Ser Ser Leu Phe Asn Phe His Ala His Val Ser Leu Pro Gln

145 150 155 160

ttt gac gaa ttg ggt tac ttg gac cca gat gac aag acc aga ttg gaa 528

Phe Asp Glu Leu Gly Tyr Leu Asp Pro Asp Asp Lys Thr Arg Leu Glu

165 170 175

gaa caa gct tct ggt ttc cca atg ttg aag gtt aag gat atc aaa tgt 576

Glu Gln Ala Ser Gly Phe Pro Met Leu Lys Val Lys Asp Ile Lys Cys

180 185 190

tct ttc tcc atg tgg aag aaa tac aag gaa tac ttt gaa aac atc acc 624

Ser Phe Ser Met Trp Lys Lys Tyr Lys Glu Tyr Phe Glu Asn Ile Thr

195 200 205

aag caa acc aag gcc tcc tcc ggt gtt atc tgg aac tct ttc aag gaa 672

Lys Gln Thr Lys Ala Ser Ser Gly Val Ile Trp Asn Ser Phe Lys Glu

210 215 220

tta gaa gaa tct gaa ttg gaa act gtt atc aga gaa att cca gct cca 720

Leu Glu Glu Ser Glu Leu Glu Thr Val Ile Arg Glu Ile Pro Ala Pro

225 230 235 240

tct ttc tta atc cca tta cca aag cat ttg act gct tct tct tcc tct 768

Ser Phe Leu Ile Pro Leu Pro Lys His Leu Thr Ala Ser Ser Ser Ser

245 250 255

ttg ttg gac cac gac aga acc gtt ttc cca tgg ttg gac caa caa cca 816

Leu Leu Asp His Asp Arg Thr Val Phe Pro Trp Leu Asp Gln Gln Pro

260 265 270

tcc aga tct gtc ttg tac gtt tct ttc ggt tct ggt act gaa gtc tta 864

Ser Arg Ser Val Leu Tyr Val Ser Phe Gly Ser Gly Thr Glu Val Leu

275 280 285

gat gaa aag gac ttc ttg gaa att gcc aga ggt tta gtc gac tcc aag 912

Asp Glu Lys Asp Phe Leu Glu Ile Ala Arg Gly Leu Val Asp Ser Lys

290 295 300

caa tct ttc ttg tgg gtt gtt aga cca ggt ttc gtc aag ggt tcc acc 960

Gln Ser Phe Leu Trp Val Val Arg Pro Gly Phe Val Lys Gly Ser Thr

305 310 315 320

tgg gtt gaa cca ttg cca gat ggt ttc ttg ggt gaa aga ggt aga att 1008

Trp Val Glu Pro Leu Pro Asp Gly Phe Leu Gly Glu Arg Gly Arg Ile

325 330 335

gtc aaa tgg gtt cct caa caa gaa gtt ttg gct cac ggt gcc att ggt 1056

Val Lys Trp Val Pro Gln Gln Glu Val Leu Ala His Gly Ala Ile Gly

340 345 350

gct ttc tgg acc cac tct ggt tgg aac tcc act ttg gaa tct gtt tgt 1104

Ala Phe Trp Thr His Ser Gly Trp Asn Ser Thr Leu Glu Ser Val Cys

355 360 365

gaa ggt gtt cca atg att ttc tcc gat ttc ggt cta gac caa cca ttg 1152

Glu Gly Val Pro Met Ile Phe Ser Asp Phe Gly Leu Asp Gln Pro Leu

370 375 380

aac gct cgt tac atg tcc gat gtt ttg aag gtt ggt gtc tac ttg gaa 1200

Asn Ala Arg Tyr Met Ser Asp Val Leu Lys Val Gly Val Tyr Leu Glu

385 390 395 400

aac ggt tgg gaa aga ggt gaa att gct aac gcc atc aga aga gtc atg 1248

Asn Gly Trp Glu Arg Gly Glu Ile Ala Asn Ala Ile Arg Arg Val Met

405 410 415

gtt gac gaa gaa ggt gaa tac atc aga caa aat gct cgt gtc ttg aaa 1296

Val Asp Glu Glu Gly Glu Tyr Ile Arg Gln Asn Ala Arg Val Leu Lys

420 425 430

caa aag gct gat gtt tct ttg atg aag ggt ggt tct tct tac gaa tcc 1344

Gln Lys Ala Asp Val Ser Leu Met Lys Gly Gly Ser Ser Tyr Glu Ser

435 440 445

ttg gaa tct tta gtc tct tac atc tcc tcc tta 1377

Leu Glu Ser Leu Val Ser Tyr Ile Ser Ser Leu

450 455

<210> 136

<211> 459

<212> PRT

<213> Stevia rebaudiana

<400> 136

Met Glu Asn Lys Thr Glu Thr Thr Val Arg Arg Arg Arg Arg Ile Ile

1 5 10 15

Leu Phe Pro Val Pro Phe Gln Gly His Ile Asn Pro Met Leu Gln Leu

20 25 30

Ala Asn Val Leu Tyr Ser Lys Gly Phe Ser Ile Thr Ile Phe His Thr

35 40 45

Asn Phe Asn Lys Pro Lys Thr Ser Asn Tyr Pro His Phe Thr Phe Arg

50 55 60

Phe Ile Leu Asp Asn Asp Pro Gln Asp Val Arg Ile Ser Asn Leu Pro

65 70 75 80

Thr His Gly Pro Leu Ala Val Met Arg Ile Leu Ile Ile Asn Glu His

85 90 95

Gly Ala Asp Glu Leu Arg Arg Glu Leu Glu Leu Leu Met Leu Ala Ser

100 105 110

Glu Glu Asp Gly Glu Val Ser Cys Leu Ile Ala Asp Gln Ile Trp Tyr

115 120 125

Phe Thr Gln Ser Val Ala Asp Ser Leu Asn Leu Arg Arg Leu Val Leu

130 135 140

Val Thr Ser Ser Leu Phe Asn Phe His Ala His Val Ser Leu Pro Gln

145 150 155 160

Phe Asp Glu Leu Gly Tyr Leu Asp Pro Asp Asp Lys Thr Arg Leu Glu

165 170 175

Glu Gln Ala Ser Gly Phe Pro Met Leu Lys Val Lys Asp Ile Lys Cys

180 185 190

Ser Phe Ser Met Trp Lys Lys Tyr Lys Glu Tyr Phe Glu Asn Ile Thr

195 200 205

Lys Gln Thr Lys Ala Ser Ser Gly Val Ile Trp Asn Ser Phe Lys Glu

210 215 220

Leu Glu Glu Ser Glu Leu Glu Thr Val Ile Arg Glu Ile Pro Ala Pro

225 230 235 240

Ser Phe Leu Ile Pro Leu Pro Lys His Leu Thr Ala Ser Ser Ser Ser

245 250 255

Leu Leu Asp His Asp Arg Thr Val Phe Pro Trp Leu Asp Gln Gln Pro

260 265 270

Ser Arg Ser Val Leu Tyr Val Ser Phe Gly Ser Gly Thr Glu Val Leu

275 280 285

Asp Glu Lys Asp Phe Leu Glu Ile Ala Arg Gly Leu Val Asp Ser Lys

290 295 300

Gln Ser Phe Leu Trp Val Val Arg Pro Gly Phe Val Lys Gly Ser Thr

305 310 315 320

Trp Val Glu Pro Leu Pro Asp Gly Phe Leu Gly Glu Arg Gly Arg Ile

325 330 335

Val Lys Trp Val Pro Gln Gln Glu Val Leu Ala His Gly Ala Ile Gly

340 345 350

Ala Phe Trp Thr His Ser Gly Trp Asn Ser Thr Leu Glu Ser Val Cys

355 360 365

Glu Gly Val Pro Met Ile Phe Ser Asp Phe Gly Leu Asp Gln Pro Leu

370 375 380

Asn Ala Arg Tyr Met Ser Asp Val Leu Lys Val Gly Val Tyr Leu Glu

385 390 395 400

Asn Gly Trp Glu Arg Gly Glu Ile Ala Asn Ala Ile Arg Arg Val Met

405 410 415

Val Asp Glu Glu Gly Glu Tyr Ile Arg Gln Asn Ala Arg Val Leu Lys

420 425 430

Gln Lys Ala Asp Val Ser Leu Met Lys Gly Gly Ser Ser Tyr Glu Ser

435 440 445

Leu Glu Ser Leu Val Ser Tyr Ile Ser Ser Leu

450 455

<210> 137

<211> 1419

<212> DNA

<213> Stevia rebaudiana

<220>

<221> CDS

<222> (1)..(1419)

<400> 137

atg gct act tct gac tcc att gtc gat gac aga aag caa ttg cac gtt 48

Met Ala Thr Ser Asp Ser Ile Val Asp Asp Arg Lys Gln Leu His Val

1 5 10 15

gcc act ttc cca tgg ttg gct ttc ggt cac att ttg cca ttc ttg caa 96

Ala Thr Phe Pro Trp Leu Ala Phe Gly His Ile Leu Pro Phe Leu Gln

20 25 30

ttg tcc aaa ttg att gct gaa aag ggt cac aag gtt tct ttc ttg tcc 144

Leu Ser Lys Leu Ile Ala Glu Lys Gly His Lys Val Ser Phe Leu Ser

35 40 45

act acc aga aac atc caa aga tta tct tct cac atc tct cca ttg atc 192

Thr Thr Arg Asn Ile Gln Arg Leu Ser Ser His Ile Ser Pro Leu Ile

50 55 60

aac gtt gtc caa ttg act ttg cca aga gtc caa gaa ttg cca gaa gat 240

Asn Val Val Gln Leu Thr Leu Pro Arg Val Gln Glu Leu Pro Glu Asp

65 70 75 80

gct gaa gct acc acc gat gtc cac cca gaa gat atc caa tac ttg aag 288

Ala Glu Ala Thr Thr Asp Val His Pro Glu Asp Ile Gln Tyr Leu Lys

85 90 95

aag gct gtc gat ggt ttg caa cca gaa gtc acc aga ttc ttg gaa caa 336

Lys Ala Val Asp Gly Leu Gln Pro Glu Val Thr Arg Phe Leu Glu Gln

100 105 110

cac tct cca gac tgg atc atc tac gac ttc acc cac tac tgg tta cca 384

His Ser Pro Asp Trp Ile Ile Tyr Asp Phe Thr His Tyr Trp Leu Pro

115 120 125

tcc att gct gct tct ttg ggt atc tcc aga gct tac ttc tgt gtc atc 432

Ser Ile Ala Ala Ser Leu Gly Ile Ser Arg Ala Tyr Phe Cys Val Ile

130 135 140

act cca tgg acc att gct tac ttg gct cca tct tct gat gcc atg atc 480

Thr Pro Trp Thr Ile Ala Tyr Leu Ala Pro Ser Ser Asp Ala Met Ile

145 150 155 160

aac gac tct gac ggt aga acc act gtt gaa gat ttg acc act cca cct 528

Asn Asp Ser Asp Gly Arg Thr Thr Val Glu Asp Leu Thr Thr Pro Pro

165 170 175

aaa tgg ttc cca ttc cca acc aag gtt tgt tgg aga aag cat gac ttg 576

Lys Trp Phe Pro Phe Pro Thr Lys Val Cys Trp Arg Lys His Asp Leu

180 185 190

gcc aga atg gaa cct tac gaa gct cca ggt att tct gat ggt tac aga 624

Ala Arg Met Glu Pro Tyr Glu Ala Pro Gly Ile Ser Asp Gly Tyr Arg

195 200 205

atg ggt atg gtt ttc aag ggt tct gac tgt cta tta ttc aaa tgt tac 672

Met Gly Met Val Phe Lys Gly Ser Asp Cys Leu Leu Phe Lys Cys Tyr

210 215 220

cat gaa ttc ggt act caa tgg ttg cca ttg ttg gaa act ttg cac caa 720

His Glu Phe Gly Thr Gln Trp Leu Pro Leu Leu Glu Thr Leu His Gln

225 230 235 240

gtt cca gtt gtt cca gtc ggt ttg ttg cca cca gaa att cca ggt gat 768

Val Pro Val Val Pro Val Gly Leu Leu Pro Pro Glu Ile Pro Gly Asp

245 250 255

gaa aag gat gaa acc tgg gtt tcc atc aag aaa tgg ttg gac ggt aag 816

Glu Lys Asp Glu Thr Trp Val Ser Ile Lys Lys Trp Leu Asp Gly Lys

260 265 270

caa aag ggt tcc gtt gtc tac gtt gct ttg ggt tct gaa gct ttg gtt 864

Gln Lys Gly Ser Val Val Tyr Val Ala Leu Gly Ser Glu Ala Leu Val

275 280 285

tct caa act gaa gtt gtc gaa ttg gct ttg ggt cta gaa ttg tcc ggt 912

Ser Gln Thr Glu Val Val Glu Leu Ala Leu Gly Leu Glu Leu Ser Gly

290 295 300

tta cca ttt gtc tgg gct tac aga aag cca aag ggt cca gcc aaa tct 960

Leu Pro Phe Val Trp Ala Tyr Arg Lys Pro Lys Gly Pro Ala Lys Ser

305 310 315 320

gat tcc gtc gaa tta cca gac ggt ttc gtc gaa aga acc cgt gac cgt 1008

Asp Ser Val Glu Leu Pro Asp Gly Phe Val Glu Arg Thr Arg Asp Arg

325 330 335

ggt tta gtc tgg act tcc tgg gct cca caa ttg aga atc ttg tcc cac 1056

Gly Leu Val Trp Thr Ser Trp Ala Pro Gln Leu Arg Ile Leu Ser His

340 345 350

gaa tcc gtt tgt ggt ttc ttg act cac tgt ggt tct ggt tct att gtc 1104

Glu Ser Val Cys Gly Phe Leu Thr His Cys Gly Ser Gly Ser Ile Val

355 360 365

gaa ggt ttg atg ttc ggt cac cca ttg att atg ttg cct ttg ttt ggt 1152

Glu Gly Leu Met Phe Gly His Pro Leu Ile Met Leu Pro Leu Phe Gly

370 375 380

gac caa cca tta aac gct aga tta ttg gaa gac aag caa gtc ggt atc 1200

Asp Gln Pro Leu Asn Ala Arg Leu Leu Glu Asp Lys Gln Val Gly Ile

385 390 395 400

gaa att cca aga aac gaa gaa gac ggt tgt ttg acc aag gaa tcc gtt 1248

Glu Ile Pro Arg Asn Glu Glu Asp Gly Cys Leu Thr Lys Glu Ser Val

405 410 415

gct cgt tct ttg aga tcc gtt gtt gtc gaa aac gaa ggt gaa atc tac 1296

Ala Arg Ser Leu Arg Ser Val Val Val Glu Asn Glu Gly Glu Ile Tyr

420 425 430

aag gcc aac gcc aga gaa ttg tct aag atc tac aac gac acc aag gtt 1344

Lys Ala Asn Ala Arg Glu Leu Ser Lys Ile Tyr Asn Asp Thr Lys Val

435 440 445

gaa aag gaa tac gtt tct caa ttt gtc gac tac ttg gaa aag aac gct 1392

Glu Lys Glu Tyr Val Ser Gln Phe Val Asp Tyr Leu Glu Lys Asn Ala

450 455 460

aga gct gtt gcc atc gac cac gaa agc 1419

Arg Ala Val Ala Ile Asp His Glu Ser

465 470

<210> 138

<211> 473

<212> PRT

<213> Stevia rebaudiana

<400> 138

Met Ala Thr Ser Asp Ser Ile Val Asp Asp Arg Lys Gln Leu His Val

1 5 10 15

Ala Thr Phe Pro Trp Leu Ala Phe Gly His Ile Leu Pro Phe Leu Gln

20 25 30

Leu Ser Lys Leu Ile Ala Glu Lys Gly His Lys Val Ser Phe Leu Ser

35 40 45

Thr Thr Arg Asn Ile Gln Arg Leu Ser Ser His Ile Ser Pro Leu Ile

50 55 60

Asn Val Val Gln Leu Thr Leu Pro Arg Val Gln Glu Leu Pro Glu Asp

65 70 75 80

Ala Glu Ala Thr Thr Asp Val His Pro Glu Asp Ile Gln Tyr Leu Lys

85 90 95

Lys Ala Val Asp Gly Leu Gln Pro Glu Val Thr Arg Phe Leu Glu Gln

100 105 110

His Ser Pro Asp Trp Ile Ile Tyr Asp Phe Thr His Tyr Trp Leu Pro

115 120 125

Ser Ile Ala Ala Ser Leu Gly Ile Ser Arg Ala Tyr Phe Cys Val Ile

130 135 140

Thr Pro Trp Thr Ile Ala Tyr Leu Ala Pro Ser Ser Asp Ala Met Ile

145 150 155 160

Asn Asp Ser Asp Gly Arg Thr Thr Val Glu Asp Leu Thr Thr Pro Pro

165 170 175

Lys Trp Phe Pro Phe Pro Thr Lys Val Cys Trp Arg Lys His Asp Leu

180 185 190

Ala Arg Met Glu Pro Tyr Glu Ala Pro Gly Ile Ser Asp Gly Tyr Arg

195 200 205

Met Gly Met Val Phe Lys Gly Ser Asp Cys Leu Leu Phe Lys Cys Tyr

210 215 220

His Glu Phe Gly Thr Gln Trp Leu Pro Leu Leu Glu Thr Leu His Gln

225 230 235 240

Val Pro Val Val Pro Val Gly Leu Leu Pro Pro Glu Ile Pro Gly Asp

245 250 255

Glu Lys Asp Glu Thr Trp Val Ser Ile Lys Lys Trp Leu Asp Gly Lys

260 265 270

Gln Lys Gly Ser Val Val Tyr Val Ala Leu Gly Ser Glu Ala Leu Val

275 280 285

Ser Gln Thr Glu Val Val Glu Leu Ala Leu Gly Leu Glu Leu Ser Gly

290 295 300

Leu Pro Phe Val Trp Ala Tyr Arg Lys Pro Lys Gly Pro Ala Lys Ser

305 310 315 320

Asp Ser Val Glu Leu Pro Asp Gly Phe Val Glu Arg Thr Arg Asp Arg

325 330 335

Gly Leu Val Trp Thr Ser Trp Ala Pro Gln Leu Arg Ile Leu Ser His

340 345 350

Glu Ser Val Cys Gly Phe Leu Thr His Cys Gly Ser Gly Ser Ile Val

355 360 365

Glu Gly Leu Met Phe Gly His Pro Leu Ile Met Leu Pro Leu Phe Gly

370 375 380

Asp Gln Pro Leu Asn Ala Arg Leu Leu Glu Asp Lys Gln Val Gly Ile

385 390 395 400

Glu Ile Pro Arg Asn Glu Glu Asp Gly Cys Leu Thr Lys Glu Ser Val

405 410 415

Ala Arg Ser Leu Arg Ser Val Val Val Glu Asn Glu Gly Glu Ile Tyr

420 425 430

Lys Ala Asn Ala Arg Glu Leu Ser Lys Ile Tyr Asn Asp Thr Lys Val

435 440 445

Glu Lys Glu Tyr Val Ser Gln Phe Val Asp Tyr Leu Glu Lys Asn Ala

450 455 460

Arg Ala Val Ala Ile Asp His Glu Ser

465 470

<210> 139

<211> 1419

<212> DNA

<213> Stevia rebaudiana

<220>

<221> CDS

<222> (1)..(1419)

<400> 139

atg gct acc tct gac tcc att gtc gat gac cgt aaa caa ttg cac gtt 48

Met Ala Thr Ser Asp Ser Ile Val Asp Asp Arg Lys Gln Leu His Val

1 5 10 15

gct act ttc cca tgg ttg gct ttc ggt cac atc ttg cct tac ttg caa 96

Ala Thr Phe Pro Trp Leu Ala Phe Gly His Ile Leu Pro Tyr Leu Gln

20 25 30

ttg tcc aag ttg att gct gaa aag ggt cac aag gtt tct ttc ttg tcc 144

Leu Ser Lys Leu Ile Ala Glu Lys Gly His Lys Val Ser Phe Leu Ser

35 40 45

act act aga aac atc caa aga tta tct tct cac atc tct cca ttg atc 192

Thr Thr Arg Asn Ile Gln Arg Leu Ser Ser His Ile Ser Pro Leu Ile

50 55 60

aac gtt gtc caa ttg act ttg cca aga gtt caa gaa tta cca gaa gat 240

Asn Val Val Gln Leu Thr Leu Pro Arg Val Gln Glu Leu Pro Glu Asp

65 70 75 80

gct gaa gct acc acc gat gtc cac cca gaa gac att cca tac ttg aag 288

Ala Glu Ala Thr Thr Asp Val His Pro Glu Asp Ile Pro Tyr Leu Lys

85 90 95

aag gcc tct gac ggt ttg caa cca gaa gtc act aga ttc ttg gaa caa 336

Lys Ala Ser Asp Gly Leu Gln Pro Glu Val Thr Arg Phe Leu Glu Gln

100 105 110

cac tct cca gac tgg atc atc tac gac tac act cac tac tgg ttg cca 384

His Ser Pro Asp Trp Ile Ile Tyr Asp Tyr Thr His Tyr Trp Leu Pro

115 120 125

tcc att gct gct tct ttg ggt atc tcc aga gcc cat ttc tcc gtc acc 432

Ser Ile Ala Ala Ser Leu Gly Ile Ser Arg Ala His Phe Ser Val Thr

130 135 140

act cca tgg gct att gct tac atg ggt cca tct gct gat gct atg atc 480

Thr Pro Trp Ala Ile Ala Tyr Met Gly Pro Ser Ala Asp Ala Met Ile

145 150 155 160

aac ggt tct gac ggt aga acc acc gtc gaa gat ttg act acc cca cct 528

Asn Gly Ser Asp Gly Arg Thr Thr Val Glu Asp Leu Thr Thr Pro Pro

165 170 175

aaa tgg ttc cca ttc cca acc aag gtt tgt tgg aga aag cat gat ttg 576

Lys Trp Phe Pro Phe Pro Thr Lys Val Cys Trp Arg Lys His Asp Leu

180 185 190

gcc aga tta gtc cca tac aag gct cca ggt att tct gat ggt tac aga 624

Ala Arg Leu Val Pro Tyr Lys Ala Pro Gly Ile Ser Asp Gly Tyr Arg

195 200 205

atg ggt ttg gtc ttg aaa ggt tct gac tgt ttg ttg tcc aaa tgt tac 672

Met Gly Leu Val Leu Lys Gly Ser Asp Cys Leu Leu Ser Lys Cys Tyr

210 215 220

cac gaa ttc ggt act caa tgg tta cca ttg ttg gaa act ttg cac caa 720

His Glu Phe Gly Thr Gln Trp Leu Pro Leu Leu Glu Thr Leu His Gln

225 230 235 240

gtt cca gtt gtt cca gtc ggt ttg ttg cct cca gaa gtt cca ggt gac 768

Val Pro Val Val Pro Val Gly Leu Leu Pro Pro Glu Val Pro Gly Asp

245 250 255

gaa aag gat gaa acc tgg gtt tcc atc aag aaa tgg ttg gat ggt aag 816

Glu Lys Asp Glu Thr Trp Val Ser Ile Lys Lys Trp Leu Asp Gly Lys

260 265 270

caa aag ggt tcc gtt gtc tac gtt gct ttg ggt tct gaa gtc ttg gtc 864

Gln Lys Gly Ser Val Val Tyr Val Ala Leu Gly Ser Glu Val Leu Val

275 280 285

agc caa act gaa gtt gtc gaa ttg gct tta ggt ttg gaa ttg tcc ggt 912

Ser Gln Thr Glu Val Val Glu Leu Ala Leu Gly Leu Glu Leu Ser Gly

290 295 300

cta cca ttt gtc tgg gct tac aga aag cca aag ggt cca gct aag tct 960

Leu Pro Phe Val Trp Ala Tyr Arg Lys Pro Lys Gly Pro Ala Lys Ser

305 310 315 320

gac tcc gtt gaa ttg cca gat ggt ttc gtc gaa aga acc aga gac aga 1008

Asp Ser Val Glu Leu Pro Asp Gly Phe Val Glu Arg Thr Arg Asp Arg

325 330 335

ggt ttg gtt tgg act tct tgg gct cca caa ttg aga atc tta tct cac 1056

Gly Leu Val Trp Thr Ser Trp Ala Pro Gln Leu Arg Ile Leu Ser His

340 345 350

gaa tct gtt tgt ggt ttc ttg acc cac tgt ggt tct ggt tcc atc gtc 1104

Glu Ser Val Cys Gly Phe Leu Thr His Cys Gly Ser Gly Ser Ile Val

355 360 365

gaa ggt ttg atg ttc ggt cac cca tta atc atg ttg cca att ttc ggt 1152

Glu Gly Leu Met Phe Gly His Pro Leu Ile Met Leu Pro Ile Phe Gly

370 375 380

gac caa cca ttg aac gcc aga tta ttg gaa gac aag caa gtc ggt att 1200

Asp Gln Pro Leu Asn Ala Arg Leu Leu Glu Asp Lys Gln Val Gly Ile

385 390 395 400

gaa att cca aga aac gaa gaa gat ggt tgt ttg acc aag gaa tcc gtt 1248

Glu Ile Pro Arg Asn Glu Glu Asp Gly Cys Leu Thr Lys Glu Ser Val

405 410 415

gcc aga tct cta cgt tct gtt gtt gtc gaa aag gaa ggt gaa atc tac 1296

Ala Arg Ser Leu Arg Ser Val Val Val Glu Lys Glu Gly Glu Ile Tyr

420 425 430

aag gcc aac gct cgt gaa tta tcc aag atc tac aac gac acc aag gtt 1344

Lys Ala Asn Ala Arg Glu Leu Ser Lys Ile Tyr Asn Asp Thr Lys Val

435 440 445

gaa aag gaa tac gtt tct caa ttt gtt gac tac ttg gaa aag aac act 1392

Glu Lys Glu Tyr Val Ser Gln Phe Val Asp Tyr Leu Glu Lys Asn Thr

450 455 460

aga gct gtt gcc att gac cat gag tct 1419

Arg Ala Val Ala Ile Asp His Glu Ser

465 470

<210> 140

<211> 473

<212> PRT

<213> Stevia rebaudiana

<400> 140

Met Ala Thr Ser Asp Ser Ile Val Asp Asp Arg Lys Gln Leu His Val

1 5 10 15

Ala Thr Phe Pro Trp Leu Ala Phe Gly His Ile Leu Pro Tyr Leu Gln

20 25 30

Leu Ser Lys Leu Ile Ala Glu Lys Gly His Lys Val Ser Phe Leu Ser

35 40 45

Thr Thr Arg Asn Ile Gln Arg Leu Ser Ser His Ile Ser Pro Leu Ile

50 55 60

Asn Val Val Gln Leu Thr Leu Pro Arg Val Gln Glu Leu Pro Glu Asp

65 70 75 80

Ala Glu Ala Thr Thr Asp Val His Pro Glu Asp Ile Pro Tyr Leu Lys

85 90 95

Lys Ala Ser Asp Gly Leu Gln Pro Glu Val Thr Arg Phe Leu Glu Gln

100 105 110

His Ser Pro Asp Trp Ile Ile Tyr Asp Tyr Thr His Tyr Trp Leu Pro

115 120 125

Ser Ile Ala Ala Ser Leu Gly Ile Ser Arg Ala His Phe Ser Val Thr

130 135 140

Thr Pro Trp Ala Ile Ala Tyr Met Gly Pro Ser Ala Asp Ala Met Ile

145 150 155 160

Asn Gly Ser Asp Gly Arg Thr Thr Val Glu Asp Leu Thr Thr Pro Pro

165 170 175

Lys Trp Phe Pro Phe Pro Thr Lys Val Cys Trp Arg Lys His Asp Leu

180 185 190

Ala Arg Leu Val Pro Tyr Lys Ala Pro Gly Ile Ser Asp Gly Tyr Arg

195 200 205

Met Gly Leu Val Leu Lys Gly Ser Asp Cys Leu Leu Ser Lys Cys Tyr

210 215 220

His Glu Phe Gly Thr Gln Trp Leu Pro Leu Leu Glu Thr Leu His Gln

225 230 235 240

Val Pro Val Val Pro Val Gly Leu Leu Pro Pro Glu Val Pro Gly Asp

245 250 255

Glu Lys Asp Glu Thr Trp Val Ser Ile Lys Lys Trp Leu Asp Gly Lys

260 265 270

Gln Lys Gly Ser Val Val Tyr Val Ala Leu Gly Ser Glu Val Leu Val

275 280 285

Ser Gln Thr Glu Val Val Glu Leu Ala Leu Gly Leu Glu Leu Ser Gly

290 295 300

Leu Pro Phe Val Trp Ala Tyr Arg Lys Pro Lys Gly Pro Ala Lys Ser

305 310 315 320

Asp Ser Val Glu Leu Pro Asp Gly Phe Val Glu Arg Thr Arg Asp Arg

325 330 335

Gly Leu Val Trp Thr Ser Trp Ala Pro Gln Leu Arg Ile Leu Ser His

340 345 350

Glu Ser Val Cys Gly Phe Leu Thr His Cys Gly Ser Gly Ser Ile Val

355 360 365

Glu Gly Leu Met Phe Gly His Pro Leu Ile Met Leu Pro Ile Phe Gly

370 375 380

Asp Gln Pro Leu Asn Ala Arg Leu Leu Glu Asp Lys Gln Val Gly Ile

385 390 395 400

Glu Ile Pro Arg Asn Glu Glu Asp Gly Cys Leu Thr Lys Glu Ser Val

405 410 415

Ala Arg Ser Leu Arg Ser Val Val Val Glu Lys Glu Gly Glu Ile Tyr

420 425 430

Lys Ala Asn Ala Arg Glu Leu Ser Lys Ile Tyr Asn Asp Thr Lys Val

435 440 445

Glu Lys Glu Tyr Val Ser Gln Phe Val Asp Tyr Leu Glu Lys Asn Thr

450 455 460

Arg Ala Val Ala Ile Asp His Glu Ser

465 470

<210> 141

<211> 2361

<212> DNA

<213> Stevia rebaudiana

<400> 141

atgaaaaccg gtttcatctc tcccgccact gttttccacc accgaatctc ccccgccacc 60

accttccgac accacctctc ccctgccacc accaactcca ctggtattgt tgctctgcga 120

gacatcaact tccgatgcaa ggctgtttcc aaggagtact ccgatctgct ccagaaggac 180

gaggcctctt tcaccaagtg ggacgacgac aaggtcaagg accacctcga caccaacaag 240

aacctctacc ccaacgacga gatcaaggag tttgtcgagt ccgtcaaggc catgttcggc 300

tccatgaacg acggcgagat taatgtctct gcttacgaca ccgcctgggt tgctctggtc 360

caggatgtcg acggttccgg ctctcctcag ttcccttcct ctctcgagtg gatcgccaac 420

aaccagctgt ccgacggttc ttggggtgac cacctgctct tctctgctca cgaccgaatc 480

atcaacaccc tggcctgtgt cattgctctg acctcttgga acgtccaccc ctccaagtgc 540

gagaagggtc tgaacttcct ccgagagaac atctgcaagc tcgaggacga gaacgccgag 600

cacatgccca ttggcttcga ggtcaccttc ccctctctga ttgacattgc caagaagctc 660

aacattgagg tccccgagga cacccccgct ctcaaggaga tctacgctcg acgagacatc 720

aagctcacca agatccccat ggaggttctc cacaaggtcc ccaccactct cctccactct 780

ctcgagggta tgcccgatct cgagtgggag aagctgctca agctgcagtg caaggacggc 840

tctttcctct tctccccctc ttccactgcc ttcgccctca tgcagaccaa ggacgagaag 900

tgtctccagt acctcaccaa cattgtcacc aagttcaacg gtggtgtccc caacgtctac 960

cccgttgacc tctttgagca catctgggtt gttgaccgac tccagcgact cggtatcgcc 1020

cgatacttca agtccgagat caaggactgt gtcgagtaca tcaacaagta ctggaccaag 1080

aacggtatct gctgggcccg aaacacccac gtccaggaca ttgacgacac cgccatgggc 1140

ttccgagttc tgcgagccca cggctacgat gtcacccccg atgtctttcg acagtttgag 1200

aaggacggca agtttgtctg tttcgccggt cagtccaccc aggccgtcac cggtatgttc 1260

aacgtctacc gagcttctca gatgctcttc cccggtgagc gaatcctcga ggacgccaag 1320

aagttctcct acaactacct caaggagaag cagtccacca acgagctgct cgacaagtgg 1380

atcattgcca aggatctgcc cggtgaggtt ggctacgccc tcgacatccc ctggtacgcc 1440

tctctgcccc gactggagac tcgatactac ctcgagcagt acggtggtga ggacgatgtc 1500

tggatcggta agaccctgta ccgaatgggc tacgtttcca acaacaccta cctcgagatg 1560

gccaagctcg actacaacaa ctacgttgcc gtcctccagc tcgagtggta caccatccag 1620

cagtggtacg tcgacattgg tatcgagaag ttcgagtccg acaacatcaa gtccgtcctt 1680

gtctcctact acctcgctgc tgcctccatc ttcgagcccg agcgatccaa ggagcgaatt 1740

gcctgggcca agaccaccat cctcgtcgac aagatcacct ccatcttcga ctcctcccag 1800

tcctccaagg aagatatcac cgccttcatt gacaagttcc gaaacaagtc ctcctccaag 1860

aagcactcca tcaacggcga gccctggcac gaggtcatgg ttgctctcaa gaaaactctc 1920

cacggctttg ccctcgacgc tctgatgacc cactctcagg acatccaccc ccagctccac 1980

caggcctggg agatgtggct caccaagctc caggacggtg ttgatgtcac tgctgagctc 2040

atggtccaga tgatcaacat gaccgccggc cgatgggttt ccaaggagct cctcacccac 2100

ccccagtacc agcgactctc cactgtcacc aactctgtct gccacgacat caccaagctc 2160

cacaacttca aggagaactc caccaccgtc gactccaagg tccaggagct ggtccagctc 2220

gttttctccg acacccccga tgatctcgac caggacatga agcagacctt cctgactgtc 2280

atgaaaactt tctactacaa ggcctggtgc gaccccaaca ccatcaacga ccacatctcc 2340

aaggtctttg agattgtgat t 2361

<210> 142

<211> 2229

<212> DNA

<213> Stevia rebaudiana

<400> 142

atgtgcaagg ctgtttccaa ggagtactcc gatctgctcc agaaggacga ggcctctttc 60

accaagtggg acgacgacaa ggtcaaggac cacctcgaca ccaacaagaa cctctacccc 120

aacgacgaga tcaaggagtt tgtcgagtcc gtcaaggcca tgttcggctc catgaacgac 180

ggcgagatta atgtctctgc ttacgacacc gcctgggttg ctctggtcca ggatgtcgac 240

ggttccggct ctcctcagtt cccttcctct ctcgagtgga tcgccaacaa ccagctgtcc 300

gacggttctt ggggtgacca cctgctcttc tctgctcacg accgaatcat caacaccctg 360

gcctgtgtca ttgctctgac ctcttggaac gtccacccct ccaagtgcga gaagggtctg 420

aacttcctcc gagagaacat ctgcaagctc gaggacgaga acgccgagca catgcccatt 480

ggcttcgagg tcaccttccc ctctctgatt gacattgcca agaagctcaa cattgaggtc 540

cccgaggaca cccccgctct caaggagatc tacgctcgac gagacatcaa gctcaccaag 600

atccccatgg aggttctcca caaggtcccc accactctcc tccactctct cgagggtatg 660

cccgatctcg agtgggagaa gctgctcaag ctgcagtgca aggacggctc tttcctcttc 720

tccccctctt ccactgcctt cgccctcatg cagaccaagg acgagaagtg tctccagtac 780

ctcaccaaca ttgtcaccaa gttcaacggt ggtgtcccca acgtctaccc cgttgacctc 840

tttgagcaca tctgggttgt tgaccgactc cagcgactcg gtatcgcccg atacttcaag 900

tccgagatca aggactgtgt cgagtacatc aacaagtact ggaccaagaa cggtatctgc 960

tgggcccgaa acacccacgt ccaggacatt gacgacaccg ccatgggctt ccgagttctg 1020

cgagcccacg gctacgatgt cacccccgat gtctttcgac agtttgagaa ggacggcaag 1080

tttgtctgtt tcgccggtca gtccacccag gccgtcaccg gtatgttcaa cgtctaccga 1140

gcttctcaga tgctcttccc cggtgagcga atcctcgagg acgccaagaa gttctcctac 1200

aactacctca aggagaagca gtccaccaac gagctgctcg acaagtggat cattgccaag 1260

gatctgcccg gtgaggttgg ctacgccctc gacatcccct ggtacgcctc tctgccccga 1320

ctggagactc gatactacct cgagcagtac ggtggtgagg acgatgtctg gatcggtaag 1380

accctgtacc gaatgggcta cgtttccaac aacacctacc tcgagatggc caagctcgac 1440

tacaacaact acgttgccgt cctccagctc gagtggtaca ccatccagca gtggtacgtc 1500

gacattggta tcgagaagtt cgagtccgac aacatcaagt ccgtccttgt ctcctactac 1560

ctcgctgctg cctccatctt cgagcccgag cgatccaagg agcgaattgc ctgggccaag 1620

accaccatcc tcgtcgacaa gatcacctcc atcttcgact cctcccagtc ctccaaggaa 1680

gatatcaccg ccttcattga caagttccga aacaagtcct cctccaagaa gcactccatc 1740

aacggcgagc cctggcacga ggtcatggtt gctctcaaga aaactctcca cggctttgcc 1800

ctcgacgctc tgatgaccca ctctcaggac atccaccccc agctccacca ggcctgggag 1860

atgtggctca ccaagctcca ggacggtgtt gatgtcactg ctgagctcat ggtccagatg 1920

atcaacatga ccgccggccg atgggtttcc aaggagctcc tcacccaccc ccagtaccag 1980

cgactctcca ctgtcaccaa ctctgtctgc cacgacatca ccaagctcca caacttcaag 2040

gagaactcca ccaccgtcga ctccaaggtc caggagctgg tccagctcgt tttctccgac 2100

acccccgatg atctcgacca ggacatgaag cagaccttcc tgactgtcat gaaaactttc 2160

tactacaagg cctggtgcga ccccaacacc atcaacgacc acatctccaa ggtctttgag 2220

attgtgatt 2229

<210> 143

<211> 2352

<212> DNA

<213> Stevia rebaudiana

<400> 143

atgaacctgt ctctctgtat cgcctctcct ctcctcacca agtccaaccg acccgctgct 60

ctgtctgcca tccacaccgc ctccacctcc cacggcggcc agaccaaccc caccaacctc 120

atcattgaca ccaccaagga gcgaatccag aagcagttca agaacgtcga gatctccgtt 180

tcctcctacg acaccgcctg ggtcgccatg gtcccctctc ccaactcccc caagtctccc 240

tgcttccccg agtgtctcaa ctggctcatc aacaaccagc tcaacgacgg ctcttggggt 300

ctggtcaacc acacccacaa ccacaaccac cccctcctca aggactctct ctcttccact 360

ctcgcctgca ttgttgctct caagcgatgg aacgttggcg aggaccagat caacaagggt 420

ctgtctttca ttgagtccaa cctcgcctcc gccaccgaga agtcccagcc ctcccccatt 480

ggctttgata tcatcttccc cggtctgctc gagtacgcca agaacctcga tatcaacctg 540

ctctccaagc agaccgactt ctctctcatg ctgcacaagc gagagctcga gcagaagcga 600

tgccactcca acgagatgga cggctacctg gcctacattt ccgagggtct gggtaacctc 660

tacgactgga acatggtcaa gaagtaccag atgaagaacg gttccgtttt caactccccc 720

tctgccaccg ctgctgcctt catcaaccac cagaaccccg gctgtctcaa ctacctcaac 780

tctctgctcg acaagtttgg taacgccgtc cccactgtct acccccacga tctcttcatc 840

cgactctcca tggtcgacac cattgagcga ctcggtattt cccaccactt ccgagtcgag 900

atcaagaacg ttctcgatga gacttaccga tgctgggttg agcgagatga gcagatcttc 960

atggacgttg tcacctgtgc tctggccttc cgactcctcc gaatcaacgg ttacgaggtt 1020

tcccccgacc ccctcgccga gatcaccaac gagctggctc tcaaggacga gtacgccgcc 1080

ctcgagactt accacgcttc tcacattctg taccaagagg atctgtcctc cggcaagcag 1140

attctcaagt ccgccgactt cctcaaggag atcatctcca ctgactccaa ccgactctcc 1200

aagctcatcc acaaggaagt cgagaacgct ctcaagttcc ccatcaacac cggtctggag 1260

cgaatcaaca cccgacgaaa catccagctc tacaacgtcg acaacacccg aattctcaag 1320

accacctacc actcttccaa catctccaac accgactacc tgcgactcgc cgtcgaggac 1380

ttctacacct gccagtccat ctaccgagag gagctcaagg gtctggagcg atgggttgtc 1440

gagaacaagc tcgaccagct caagtttgcc cgacaaaaga ctgcctactg ctacttctcc 1500

gttgctgcca ccctctcttc tcccgagctc tccgacgccc gaatctcttg ggccaagaac 1560

ggtatcctga ccactgttgt cgacgacttc tttgacattg gtggcaccat tgacgagctg 1620

accaacctca tccagtgcgt cgagaagtgg aacgtcgacg ttgacaagga ctgttgttcc 1680

gagcacgtcc gaatcctctt cctggctctc aaggacgcca tctgctggat cggtgacgag 1740

gccttcaagt ggcaggctcg agatgtcact tcccacgtca tccagacctg gctcgagctc 1800

atgaactcca tgctgcgaga ggccatctgg acccgagatg cctacgtccc caccctcaac 1860

gagtacatgg agaacgccta cgtcagcttt gctctcggtc ccattgtcaa gcccgccatc 1920

tactttgtcg gtcccaagct gtccgaggag attgtcgagt cctccgagta ccacaacctc 1980

ttcaagctca tgtccaccca gggccgactc ctcaacgata tccactcctt caagcgagag 2040

ttcaaggaag gtaagctcaa cgccgttgct ctgcacctgt ccaacggtga gtccggcaag 2100

gtcgaggaag aggtcgtcga ggagatgatg atgatgatca agaacaagcg aaaggagctc 2160

atgaagctca tcttcgagga gaacggctcc attgtccccc gagcctgcaa ggacgccttc 2220

tggaacatgt gccacgtcct caacttcttc tacgccaacg acgacggttt caccggcaac 2280

accattctcg acaccgtcaa ggacatcatc tacaaccctc tggttctggt caacgagaac 2340

gaggagcaga gg 2352

<210> 144

<211> 2271

<212> DNA

<213> Stevia rebaudiana

<400> 144

atgacctccc acggcggcca gaccaacccc accaacctca tcattgacac caccaaggag 60

cgaatccaga agcagttcaa gaacgtcgag atctccgttt cctcctacga caccgcctgg 120

gtcgccatgg tcccctctcc caactccccc aagtctccct gcttccccga gtgtctcaac 180

tggctcatca acaaccagct caacgacggc tcttggggtc tggtcaacca cacccacaac 240

cacaaccacc ccctcctcaa ggactctctc tcttccactc tcgcctgcat tgttgctctc 300

aagcgatgga acgttggcga ggaccagatc aacaagggtc tgtctttcat tgagtccaac 360

ctcgcctccg ccaccgagaa gtcccagccc tcccccattg gctttgatat catcttcccc 420

ggtctgctcg agtacgccaa gaacctcgat atcaacctgc tctccaagca gaccgacttc 480

tctctcatgc tgcacaagcg agagctcgag cagaagcgat gccactccaa cgagatggac 540

ggctacctgg cctacatttc cgagggtctg ggtaacctct acgactggaa catggtcaag 600

aagtaccaga tgaagaacgg ttccgttttc aactccccct ctgccaccgc tgctgccttc 660

atcaaccacc agaaccccgg ctgtctcaac tacctcaact ctctgctcga caagtttggt 720

aacgccgtcc ccactgtcta cccccacgat ctcttcatcc gactctccat ggtcgacacc 780

attgagcgac tcggtatttc ccaccacttc cgagtcgaga tcaagaacgt tctcgatgag 840

acttaccgat gctgggttga gcgagatgag cagatcttca tggacgttgt cacctgtgct 900

ctggccttcc gactcctccg aatcaacggt tacgaggttt cccccgaccc cctcgccgag 960

atcaccaacg agctggctct caaggacgag tacgccgccc tcgagactta ccacgcttct 1020

cacattctgt accaagagga tctgtcctcc ggcaagcaga ttctcaagtc cgccgacttc 1080

ctcaaggaga tcatctccac tgactccaac cgactctcca agctcatcca caaggaagtc 1140

gagaacgctc tcaagttccc catcaacacc ggtctggagc gaatcaacac ccgacgaaac 1200

atccagctct acaacgtcga caacacccga attctcaaga ccacctacca ctcttccaac 1260

atctccaaca ccgactacct gcgactcgcc gtcgaggact tctacacctg ccagtccatc 1320

taccgagagg agctcaaggg tctggagcga tgggttgtcg agaacaagct cgaccagctc 1380

aagtttgccc gacaaaagac tgcctactgc tacttctccg ttgctgccac cctctcttct 1440

cccgagctct ccgacgcccg aatctcttgg gccaagaacg gtatcctgac cactgttgtc 1500

gacgacttct ttgacattgg tggcaccatt gacgagctga ccaacctcat ccagtgcgtc 1560

gagaagtgga acgtcgacgt tgacaaggac tgttgttccg agcacgtccg aatcctcttc 1620

ctggctctca aggacgccat ctgctggatc ggtgacgagg ccttcaagtg gcaggctcga 1680

gatgtcactt cccacgtcat ccagacctgg ctcgagctca tgaactccat gctgcgagag 1740

gccatctgga cccgagatgc ctacgtcccc accctcaacg agtacatgga gaacgcctac 1800

gtcagctttg ctctcggtcc cattgtcaag cccgccatct actttgtcgg tcccaagctg 1860

tccgaggaga ttgtcgagtc ctccgagtac cacaacctct tcaagctcat gtccacccag 1920

ggccgactcc tcaacgatat ccactccttc aagcgagagt tcaaggaagg taagctcaac 1980

gccgttgctc tgcacctgtc caacggtgag tccggcaagg tcgaggaaga ggtcgtcgag 2040

gagatgatga tgatgatcaa gaacaagcga aaggagctca tgaagctcat cttcgaggag 2100

aacggctcca ttgtcccccg agcctgcaag gacgccttct ggaacatgtg ccacgtcctc 2160

aacttcttct acgccaacga cgacggtttc accggcaaca ccattctcga caccgtcaag 2220

gacatcatct acaaccctct ggttctggtc aacgagaacg aggagcagag g 2271

<210> 145

<211> 1539

<212> DNA

<213> Stevia rebaudiana

<400> 145

atggacgccg tcactggtct gctcaccgtc cccgccactg ccatcaccat cggtggtact 60

gccgttgctc tggccgttgc tctcatcttc tggtacctca agtcctacac ctctgcccga 120

cgatcccagt ccaaccacct cccccgagtc cccgaggtcc ccggtgtccc cctgctcggt 180

aacctgctcc agctcaagga gaagaagccc tacatgacct tcacccgatg ggctgccacc 240

tacggcccca tctactccat caagactggt gccacctcca tggttgttgt ctcttccaac 300

gagattgcta aagaggctct cgtcacccga ttccagtcca tctccacccg aaacctctcc 360

aaggctctca aggttctgac cgccgacaag accatggttg ccatgtccga ctacgacgac 420

taccacaaga ccgtcaagcg acacattctc accgccgttc tcggccccaa cgcccagaag 480

aagcaccgaa tccaccgaga catcatgatg gacaacatct ccacccagct ccacgagttt 540

gtcaagaaca accccgagca ggaagaggtt gatctccgaa agatcttcca gtccgagctc 600

ttcggtctgg ccatgcgaca ggctctgggc aaggatgtcg agtctctcta cgtcgaggat 660

ctcaagatca ccatgaaccg agatgagatc ttccaggttc tggttgtcga ccccatgatg 720

ggtgccattg atgtcgactg gcgagacttc ttcccctacc tcaagtgggt ccccaacaag 780

aagtttgaga acaccatcca gcagatgtac atccgacgag aggctgtcat gaagtctctc 840

atcaaggaga acaagaagcg aattgcctcc ggtgagaagc tcaactccta cattgactac 900

ctgctctccg aggcccagac cctcaccgac cagcagctgc tcatgtctct gtgggagccc 960

atcattgagt cctccgacac caccatggtc accaccgagt gggccatgta cgagctcgcc 1020

aagaacccca agctccagga ccgactctac cgagacatca agtctgtctg tggctccgag 1080

aagatcaccg aggagcacct gtcccagctc ccctacatca ctgccatctt ccacgagact 1140

ctccgacgac actctcccgt ccccatcatc cctctccgac acgtccacga ggacaccgtt 1200

ctgggtggct accacgtccc cgccggtacc gagctcgctg tcaacatcta cggctgcaac 1260

atggacaaga acgtctggga gaaccccgag gagtggaacc ccgagcgatt catgaaggag 1320

aacgagacta ttgacttcca gaaaaccatg gcctttggtg gtggcaagcg agtctgcgcc 1380

ggctctctcc aggctctgct gaccgcctcc attggtattg gccgaatggt ccaggagttc 1440

gagtggaagc tcaaggacat gacccaggaa gaggtcaaca ccattggcct caccacccag 1500

atgctccgac ctctccgagc catcatcaag ccccgaata 1539

<210> 146

<211> 1566

<212> DNA

<213> Stevia rebaudiana

<400> 146

atgggtctgt tccccctcga ggactcctac gctctggtct ttgagggcct cgccatcacc 60

ctcgccctct actacctgct ctccttcatc tacaagacct ccaagaaaac ctgcactcct 120

cccaaggcct ccggtgagca ccccatcacc ggccacctca acctgctctc cggctcttcc 180

ggcctccccc acctcgccct cgcctctctg gctgaccgat gtggtcccat cttcaccatc 240

cgactcggta tccgacgagt cctcgttgtc tccaactggg agattgccaa ggagatcttc 300

accacccacg atctcattgt ctccaaccga cccaagtacc tggctgccaa gattctcggt 360

ttcaactacg tttccttctc ctttgccccc tacggcccct actgggtcgg tatccgaaag 420

atcattgcca ccaagctcat gtcctcttct cgactccaga agctccagtt cgtccgagtc 480

tttgagctcg agaactccat gaagtccatc cgagagtctt ggaaggagaa gaaggacgag 540

gaaggcaagg ttctggtcga gatgaagaag tggttctggg agctcaacat gaacattgtt 600

ctgcgaactg ttgctggtaa gcagtacacc ggtaccgtcg acgacgccga tgccaagcga 660

atctccgagc tcttccgaga gtggttccac tacaccggcc gatttgttgt cggtgacgcc 720

ttccccttcc tcggctggct cgatctcggt ggctacaaga aaaccatgga gctcgtcgcc 780

tcccgactcg actccatggt cagcaagtgg ctcgacgagc accgaaagaa gcaggccaac 840

gacgacaaga aagaggacat ggacttcatg gacatcatga tctccatgac cgaggccaac 900

tctcccctcg agggttacgg taccgacacc atcatcaaga ccacctgcat gaccctcatt 960

gtctccggtg tcgacaccac ctccattgtc ctgacctggg ctctgtctct gctgctcaac 1020

aaccgagaca ctctcaagaa ggcccaggaa gagctcgaca tgtgtgtcgg caagggccga 1080

caggtcaacg agtccgatct ggtcaacctc atctacctcg aggctgttct caaggaagct 1140

ctgcgactct accccgctgc cttcctcggt ggtccccgag ccttcctcga ggactgcact 1200

gttgccggct accgaatccc caagggtacc tgtctgctca tcaacatgtg gaagctccac 1260

cgagatccca acatctggtc cgacccctgc gagttcaagc ccgagcgatt cctgaccccc 1320

aaccagaagg acgttgatgt cattggtatg gacttcgagc tcatcccctt cggtgccggc 1380

cgacgatact gccccggtac ccgactcgcc ctccagatgc tgcacattgt tctcgccact 1440

ctgctccaga actttgagat gtccaccccc aacgacgccc ccgtcgacat gactgcttcc 1500

gtcggtatga ccaacgccaa ggcttctcct ctcgaggtcc tcctgtctcc ccgagtcaag 1560

tggtca 1566

<210> 147

<211> 1443

<212> DNA

<213> Stevia rebaudiana

<400> 147

atggacgcca tggccaccac cgagaagaag ccccacgtca tcttcatccc cttccccgcc 60

cagtcccaca tcaaggccat gctcaagctc gcccagctcc tccaccacaa gggcctccag 120

atcacctttg tcaacaccga cttcatccac aaccagttcc tcgagtcctc cggcccccac 180

tgtctggacg gtgctcccgg tttccgattt gagactatcc ccgatggtgt ctcccactcc 240

cccgaggcct ccatccccat ccgagagtct ctgctccgat ccattgagac taacttcctc 300

gaccgattca ttgatctcgt caccaagctc cccgatcctc ccacctgtat catctccgac 360

ggtttcctgt ccgttttcac cattgatgct gccaagaagc tcggtatccc cgtcatgatg 420

tactggactc tggctgcctg tggtttcatg ggtttctacc acatccactc tctgatcgag 480

aagggctttg ctcctctcaa ggacgcctcc tacctcacca acggttacct cgacaccgtc 540

attgactggg tccccggtat ggagggtatc cgactcaagg acttccccct cgactggtcc 600

accgacctca acgacaaggt tctcatgttc accaccgagg ctccccagcg atcccacaag 660

gtttcccacc acatcttcca caccttcgac gagctcgagc cctccatcat caagactctg 720

tctctgcgat acaaccacat ctacaccatt ggccccctcc agctcctcct cgaccagatc 780

cccgaggaga agaagcagac cggtatcacc tctctgcacg gctactctct cgtcaaggaa 840

gagcccgagt gcttccagtg gctccagtcc aaggagccca actccgttgt ctacgtcaac 900

tttggctcca ccaccgtcat gtctctcgag gacatgaccg agtttggctg gggtctggcc 960

aactccaacc actacttcct gtggatcatc cgatccaacc tcgtcattgg cgagaacgcc 1020

gttctgcctc ccgagctcga ggagcacatc aagaagcgag gcttcattgc ctcttggtgc 1080

tcccaggaga aggttctcaa gcacccctcc gtcggtggtt tcctgaccca ctgcggctgg 1140

ggctccacca ttgagtctct gtccgctggt gtccccatga tctgctggcc ctactcctgg 1200

gaccagctca ccaactgccg atacatctgc aaggagtggg aggttggtct ggagatgggt 1260

accaaggtca agcgagatga ggtcaagcga ctcgtccagg agctcatggg cgagggtggt 1320

cacaagatgc gaaacaaggc caaggactgg aaggagaagg cccgaattgc cattgccccc 1380

aacggctctt cttctctcaa cattgacaag atggtcaagg agatcactgt tctcgctcga 1440

aac 1443

<210> 148

<211> 1380

<212> DNA

<213> Stevia rebaudiana

<400> 148

atggccgagc agcagaagat caagaagtct ccccacgttc tgctcatccc cttccctctg 60

cagggccaca tcaacccctt catccagttc ggcaagcgac tcatctccaa gggtgtcaag 120

accactctgg tcaccaccat ccacaccctc aactccactc tcaaccactc caacaccacc 180

accacctcca tcgagatcca ggccatctcc gacggctgtg acgagggtgg tttcatgtct 240

gctggtgagt cttacctcga gactttcaag caggtcggtt ccaagtctct ggctgacctc 300

atcaagaagc tccagtccga gggtaccacc attgacgcca tcatctacga ctccatgacc 360

gagtgggttc tcgatgtcgc catcgagttt ggtattgacg gtggctcctt cttcacccag 420

gcctgtgtcg tcaactctct ctactaccac gtccacaagg gtctgatctc tctgcccctc 480

ggcgagactg tctccgtccc cggtttcccc gttctgcagc gatgggagac tcctctcatt 540

ctccagaacc acgagcagat ccagtccccc tggtcccaga tgctcttcgg ccagttcgcc 600

aacattgacc aggcccgatg ggttttcacc aactccttct acaagctcga ggaagaggtc 660

attgagtgga cccgaaagat ctggaacctc aaggtcattg gccccaccct cccctccatg 720

tacctcgaca agcgactcga tgacgacaag gacaacggtt tcaacctcta caaggccaac 780

caccacgagt gcatgaactg gctcgacgac aagcccaagg agtccgttgt ctacgttgcc 840

tttggctctc tggtcaagca cggccccgag caggttgagg agatcacccg agctctgatt 900

gactccgatg tcaacttcct gtgggtcatc aagcacaagg aagagggtaa gctccccgag 960

aacctgtccg aggtcatcaa gaccggcaag ggcctcattg ttgcctggtg caagcagctc 1020

gacgttctcg cccacgagtc cgtcggctgc tttgtcaccc actgcggttt caactccacc 1080

ctcgaggcta tctctctcgg tgtccccgtt gttgccatgc cccagttctc cgaccagacc 1140

accaacgcca agctcctcga tgagattctc ggtgtcggtg tccgagtcaa ggctgacgag 1200

aacggtattg tccgacgagg taacctggct tcttgtatca agatgatcat ggaggaagag 1260

cgaggtgtca tcatccgaaa gaacgccgtc aagtggaagg atctggccaa ggttgctgtc 1320

cacgagggtg gctcttccga caacgacatt gtcgagtttg tctccgagct catcaaggcc 1380

<210> 149

<211> 1374

<212> DNA

<213> Stevia rebaudiana

<400> 149

atggagaaca agaccgagac taccgtccga cgacgacgac gaatcattct cttccccgtc 60

cccttccagg gccacatcaa ccccattctg cagctcgcca acgttctgta ctccaagggc 120

ttctccatca ccatcttcca caccaacttc aacaagccca agacctccaa ctacccccac 180

ttcactttcc gattcatcct cgacaacgac ccccaggacg agcgaatctc caacctgccc 240

acccacggtc ctctggctgg tatgcgaatc cccatcatca acgagcacgg tgctgacgag 300

ctccgacgag agctcgagct gctcatgctc gcctccgaag aggacgagga agtctcctgt 360

ctgatcaccg atgctctgtg gtactttgcc cagtccgtcg ccgactctct caacctgcga 420

cgactcgttc tcatgacctc ctctctgttc aacttccacg cccacgtttc tctgccccag 480

tttgacgagc tcggttacct cgaccccgat gacaagaccc gactcgagga gcaggcttcc 540

ggtttcccca tgctcaaggt caaggacatc aagtccgcct actccaactg gcagattctc 600

aaggagattc tcggcaagat gatcaagcag accaaggcct cctccggtgt catctggaac 660

tccttcaagg agctcgagga gtccgagctc gagactgtca tccgagagat ccccgctccc 720

tctttcctca tccccctgcc caagcacctc accgcttcct cctcttctct gctcgaccac 780

gaccgaaccg tctttcagtg gctcgaccag cagccccctt cctccgtcct ctacgtttcc 840

ttcggctcca cctccgaggt cgacgagaag gacttcctcg agattgctcg aggcctcgtt 900

gactccaagc agtccttcct gtgggttgtc cgacccggct ttgtcaaggg ctccacctgg 960

gttgagcccc tgcccgatgg tttcctcggt gagcgaggcc gaattgtcaa gtgggtcccc 1020

cagcaggaag ttctggccca cggtgccatt ggtgccttct ggacccactc cggctggaac 1080

tccactctcg agtccgtctg cgagggtgtc cccatgatct tctccgactt tggcctcgac 1140

cagcccctca acgcccgata catgtccgat gttctcaagg tcggtgtcta cctcgagaac 1200

ggctgggagc gaggtgagat tgccaacgcc atccgacgag tcatggtcga cgaggaaggt 1260

gagtacatcc gacagaacgc ccgagtcctc aagcagaagg ccgatgtctc tctcatgaag 1320

ggtggttctt cttacgagtc tctcgagtct ctcgtttcct acatctcttc tttg 1374

<210> 150

<211> 2130

<212> DNA

<213> Stevia rebaudiana

<400> 150

atgcagtccg actccgtcaa ggtcagcccc ttcgatcttg tctccgccgc catgaacggc 60

aaggccatgg agaagctcaa cgcctccgag tccgaggacc ccaccaccct ccccgctctc 120

aagatgctcg tcgagaaccg agagctcctc accctcttca ccacctcctt cgccgttctc 180

attggctgtc tggtctttct catgtggcga cgatcttcct ccaagaagct cgtccaggac 240

cccgtccccc aggtcattgt tgtcaagaag aaggagaagg agtccgaggt cgacgacggc 300

aagaagaagg tttccatctt ctacggtact cagaccggta ctgccgaggg tttcgccaag 360

gctctcgtcg aggaagccaa ggtccgatac gaaaagacct ccttcaaggt catcgatctc 420

gatgactacg ctgctgacga cgacgagtac gaggagaagc tcaagaagga gtctctcgcc 480

ttcttcttcc tggccaccta cggtgacggt gagcccactg acaacgccgc caacttctac 540

aagtggttca ccgagggtga cgacaagggc gagtggctca agaagctcca gtacggtgtc 600

tttggcctcg gtaaccgaca gtacgagcac ttcaacaaga ttgccattgt tgtcgacgac 660

aagctgaccg agatgggcgc caagcgactc gttcccgtcg gcctcggtga tgatgaccag 720

tgtatcgagg acgacttcac cgcctggaag gagctcgtct ggcccgagct cgaccagctg 780

ctgcgagatg aggacgacac ttccgtcacc actccctaca ccgctgctgt tctcgagtac 840

cgagttgtct accacgacaa gcccgctgac tcctacgccg aggaccagac ccacaccaac 900

ggccacgttg tccacgacgc ccagcacccc tctcgatcca acgttgcctt caagaaggag 960

ctccacacct cccagtccga ccgatcttgc acccacctcg agtttgacat ctcccacact 1020

ggtctgtctt acgaaaccgg cgaccacgtc ggtgtctact ccgagaacct ctccgaggtt 1080

gtcgacgagg ctctcaagct gctcggtctg tctcccgaca cctacttctc cgtccacgcc 1140

gataaagagg acggcacccc catcggtggt gcttctctgc ctcctccctt tcccccttgc 1200

actctgcgag atgctctgac ccgatacgcc gacgttctct cttctcccaa gaaggttgct 1260

ctgctggccc tcgccgccca cgcctccgac ccctccgagg ccgaccgact caagttcctc 1320

gcctctcccg ctggtaagga cgagtacgcc cagtggatcg tcgccaacca gcgatctctg 1380

ctcgaggtca tgcagtcctt cccctctgcc aagccccctc tgggtgtttt cttcgccgcc 1440

gttgctcccc gactccagcc ccgatactac tccatctctt cttctcccaa gatgtccccc 1500

aaccgaatcc acgtcacctg tgctctggtc tacgaaacca cccccgccgg ccgaatccac 1560

cgaggcctct gttccacctg gatgaagaac gccgtccccc tgaccgagtc ccccgactgc 1620

tcccaggctt ccatcttcgt ccgaacctcc aacttccgac tccccgttga ccccaaggtc 1680

cccgtcatca tgatcggtcc cggtaccggt ctggccccct tccgaggctt cctgcaggag 1740

cgactcgctc tcaaggagtc cggcaccgag ctcggttcct ccatcttctt ctttggctgc 1800

cgaaaccgaa aggtcgactt catctacgag gacgagctca acaactttgt cgagactggt 1860

gctctctccg agctcattgt tgccttctcc cgagagggta ccgccaagga gtacgtccag 1920

cacaagatgt cccagaaggc ctccgacatc tggaagctcc tctccgaggg tgcctacctc 1980

tacgtctgcg gtgacgccaa gggtatggcc aaggatgtcc accgaaccct gcacaccatt 2040

gtccaggagc agggctctct cgactcttcc aaggccgagc tctacgtcaa gaacctccag 2100

atgtccggcc gatacctccg agatgtgtgg 2130

<210> 151

<211> 2397

<212> DNA

<213> Lactuca sativa

<400> 151

atgaaaacca tgatctcttc tcccatcccc gctttccacc cccgattctc tcctgctgct 60

ggctctcgac gactctcccc catcctcccc tcttccggct ccgtcgttct gaccggctcc 120

aagacccagt gcaaggctgt ctccaagtcc cccacccagg agtacttcga cgttctgcag 180

aagaacggtc tgcccttcat caactggcag aacgacgttg tcgaggacga gctcgacaag 240

gagaagaaga ttctctaccc caacgacgag atcaagggct ttgtcgagcg aatcaaggtc 300

atgctcggct ccatggacga gggtgagatc actgtctccg cctacgacac cgcctgggtt 360

gctctcgtcc aggacattga cggcaacggt cgacccgagt tcccctcctc tctcgagtgg 420

atcgtcaaga accagctgtc cgacggctct tggggtgacc acctcatctt ctccgcccac 480

gaccgaatca tcaacaccct cgcctgtgtc attgctctga cctcttggaa cgtccacccc 540

ggcaagtgcc agaagggtct gaagttcctc aacgacaaca tctccaagct cgaagaggag 600

aaccccgagc acatgcccat tggtttcgag gttgccttcc cctctctcat tgacattgcc 660

cgaaagctcg atatccaggt ccccgaggac tcccctgctc tcaaggagat ctacgcccga 720

cgaaacctca agctcaccaa gatccccaag tctctcatgc acaaggtccc caccactctg 780

ctgcactctc tcgagggtat gcccgatctc gagtgggaga agctgctcaa gctccagtgc 840

aaggacggtt ccttcctctt ctccccctcc tccaccgcct tcgccctcat gcagaccaag 900

gaccagaagt gtctgcagta cctcaccgac gccgtcacca agttcaacgg tggtgtcccc 960

aacgtctacc ccgtcgacct ctttgagcac atctgggttg tcgaccgact gcagcgactc 1020

ggtatctctc gatactttga ctccgagatc aaggactgtg tcgactacat ctaccgatac 1080

tggaccaagg acggtatctg ctgggccaag aactccaacg tccaggacat tgacgacacc 1140

gccatgggct tccgagttct gcgaatgcac ggctacaagg tcaccactga cgttttccga 1200

cagttcgaga aggacggcaa gttcgtctgt ttccccggcc agaccaccca ggccgtcacc 1260

ggtatgttca acctcttccg agcttcccag gttctcttcc ccgacgagaa gattctcgag 1320

gacgccaaga agttctccta caactacctc aaggagaagc agtccaccaa cgagctcctc 1380

gacaagtgga tcattgccaa ggatctgccc ggtgaggtcg agtacgccct cgatgtcccc 1440

tggtacgcct ctctgcctcg actcgagact cgattctacc tcgagcagta cggtggcgag 1500

gacgatgtct ggatcggtaa gactctctac cgaatgggta acgtttccaa caacacctac 1560

ctcgagatgg ccaagctcga ctacaacaac tgtctggcca tccaccacct cgagtggaac 1620

accatgcagc agtggtacgt cgactttggt atggagcgat tcggtacctc cgatatcacc 1680

tctctgcttg tctcttacta cctcgccgct gcctccgtct ttgagcccga gcgatccaag 1740

gagcgaattg cctgggccaa gaccaccact ctggtcgaca ccatctcctc cttcttccac 1800

tctctcaaga tctccaacga gcaccgacga gagtttgtcg aggagttccg aaacatctcc 1860

aactccatcc accacgccaa gtacggcaag ccctggcacg gtctgatggt tgctctcaag 1920

ggtactctgc acgagattgc tctcgatgtt ctcatgaccc accgacgaga catccacccc 1980

cagctccacc acgcctggga gatgtggctc atgcgatggc agcagggtgt tgacgccacc 2040

gagggccagg ctgagctcat tgtccagacc atcaacatga ccgccggtcg atgggtttcc 2100

aacgagctgc tcgcccaccc ccagtaccga ctcctctcct ccgtcatcaa caacatctgc 2160

cacgagatct accacaaccg aacctgcatg gaggtcaact ccaccaccat ctccacctcc 2220

attgactcca agatgcagga gctcgtccag ctggttctct ccgactctct cgatgatctc 2280

gaccaggatc tcaagcagac cttcctgacc gttgccaaga ccttctacta caaggcctac 2340

tgcgaccccg agactatcaa cgtccacatc tccaaggtca tgtttgagac tattatt 2397

<210> 152

<211> 2271

<212> DNA

<213> Lactuca sativa

<400> 152

atgtgcaagg ctgtctccaa gtcccccacc caggagtact tcgacgttct gcagaagaac 60

ggtctgccct tcatcaactg gcagaacgac gttgtcgagg acgagctcga caaggagaag 120

aagattctct accccaacga cgagatcaag ggctttgtcg agcgaatcaa ggtcatgctc 180

ggctccatgg acgagggtga gatcactgtc tccgcctacg acaccgcctg ggttgctctc 240

gtccaggaca ttgacggcaa cggtcgaccc gagttcccct cctctctcga gtggatcgtc 300

aagaaccagc tgtccgacgg ctcttggggt gaccacctca tcttctccgc ccacgaccga 360

atcatcaaca ccctcgcctg tgtcattgct ctgacctctt ggaacgtcca ccccggcaag 420

tgccagaagg gtctgaagtt cctcaacgac aacatctcca agctcgaaga ggagaacccc 480

gagcacatgc ccattggttt cgaggttgcc ttcccctctc tcattgacat tgcccgaaag 540

ctcgatatcc aggtccccga ggactcccct gctctcaagg agatctacgc ccgacgaaac 600

ctcaagctca ccaagatccc caagtctctc atgcacaagg tccccaccac tctgctgcac 660

tctctcgagg gtatgcccga tctcgagtgg gagaagctgc tcaagctcca gtgcaaggac 720

ggttccttcc tcttctcccc ctcctccacc gccttcgccc tcatgcagac caaggaccag 780

aagtgtctgc agtacctcac cgacgccgtc accaagttca acggtggtgt ccccaacgtc 840

taccccgtcg acctctttga gcacatctgg gttgtcgacc gactgcagcg actcggtatc 900

tctcgatact ttgactccga gatcaaggac tgtgtcgact acatctaccg atactggacc 960

aaggacggta tctgctgggc caagaactcc aacgtccagg acattgacga caccgccatg 1020

ggcttccgag ttctgcgaat gcacggctac aaggtcacca ctgacgtttt ccgacagttc 1080

gagaaggacg gcaagttcgt ctgtttcccc ggccagacca cccaggccgt caccggtatg 1140

ttcaacctct tccgagcttc ccaggttctc ttccccgacg agaagattct cgaggacgcc 1200

aagaagttct cctacaacta cctcaaggag aagcagtcca ccaacgagct cctcgacaag 1260

tggatcattg ccaaggatct gcccggtgag gtcgagtacg ccctcgatgt cccctggtac 1320

gcctctctgc ctcgactcga gactcgattc tacctcgagc agtacggtgg cgaggacgat 1380

gtctggatcg gtaagactct ctaccgaatg ggtaacgttt ccaacaacac ctacctcgag 1440

atggccaagc tcgactacaa caactgtctg gccatccacc acctcgagtg gaacaccatg 1500

cagcagtggt acgtcgactt tggtatggag cgattcggta cctccgatat cacctctctg 1560

cttgtctctt actacctcgc cgctgcctcc gtctttgagc ccgagcgatc caaggagcga 1620

attgcctggg ccaagaccac cactctggtc gacaccatct cctccttctt ccactctctc 1680

aagatctcca acgagcaccg acgagagttt gtcgaggagt tccgaaacat ctccaactcc 1740

atccaccacg ccaagtacgg caagccctgg cacggtctga tggttgctct caagggtact 1800

ctgcacgaga ttgctctcga tgttctcatg acccaccgac gagacatcca cccccagctc 1860

caccacgcct gggagatgtg gctcatgcga tggcagcagg gtgttgacgc caccgagggc 1920

caggctgagc tcattgtcca gaccatcaac atgaccgccg gtcgatgggt ttccaacgag 1980

ctgctcgccc acccccagta ccgactcctc tcctccgtca tcaacaacat ctgccacgag 2040

atctaccaca accgaacctg catggaggtc aactccacca ccatctccac ctccattgac 2100

tccaagatgc aggagctcgt ccagctggtt ctctccgact ctctcgatga tctcgaccag 2160

gatctcaagc agaccttcct gaccgttgcc aagaccttct actacaaggc ctactgcgac 2220

cccgagacta tcaacgtcca catctccaag gtcatgtttg agactattat t 2271

<210> 153

<211> 2283

<212> DNA

<213> Picea glauca

<400> 153

atgaagatgt ccaagtccgt cgaggtccag cactgcgccg tccagttcct gtcctccacc 60

accgaccaga ttgagatccg agagcgaaac ctccagatct ccaccgaggc catgaagatg 120

aagtcttgga tcgagactgt caagtacatt ctgcagtcca tggaggacgg tgagatcacc 180

atctctgctt acgacactgc ctggatcgcc ctcgtccccg ctctcaacgg ctcttccgag 240

ccccagttcc cctcttctct gcagtggctc atcaacaacc agctccagga cggctcttgg 300

ggtgaccccc tcatgttcct catccgagat cgaatcatca acaccctcgc ctgtgtcctt 360

gctctcaaga cctggaacat ccactctctc ggtgtcaaca agggtctgtc cttcctccag 420

acctacatcc ccaagatgaa cgacgagcac gacgcccaca cccccgttgg ctttgagatt 480

gtctttcctg ctctcatgga ggacgccaag atcatggagc tcgatctgcc ctacgacgcc 540

gagttcctcc agaagatcta cgacgagcga gatctcaaga tgaagcgaat ccccatgaag 600

gttctgcacg agttcccctc cactctgctc cactctctcg agggcctccg agacaaggtc 660

aactgggagg agctcctcaa gctccagtcc aagaacggtt ccttcctctt ctcccccgcc 720

tccaccgcct gtgctctggc ccagacctcc gacaccaact gtctgcgata cctcaacgag 780

atcaccaaga agtacgacgg tggtgctccc aacgtctacc ccgttgacct ctttgagcga 840

ctctggaccg tcgaccgaat tgagcgactc ggtattgccc gatactttga gtccgagatc 900

accgactctc tcgagtacgt ctaccgatac tggaccaacc agggtattgg ctgggcccga 960

gactcccccg tcaaggacgt tgacgacacc tccatggcct tccgactgct gcgatcccac 1020

ggcttcgatg tcactgccga ggccttcaac cacttcaagc aggacgacca gttcttctgc 1080

ttctttggcc agaccaagca gaccgtcacc ggtatgtaca acctctaccg agcctcccag 1140

ttctccttcc ccggtgagtc catcctcgag gaagctcgag tctttaccaa gaacttcctc 1200

gaggagaagc gagctgagaa gcagctgcga gacaagtgga tcattgccaa gggcctcaag 1260

gaagaggtcg agtacgctct caagttcccc tggtacgcct ctcagcctcg aattgacacc 1320

cgaatgtaca tcaaccagta ccgagttgat gatgtctgga tcggtaaggc tctctaccga 1380

atgcccattg tcaacaacaa gacctacatt gagctcgcca aggctgactt caacatctgc 1440

cagtccatcc accgaaccga gctccacggt atcatccgat ggtaccgaga gtccggtctg 1500

gacgagctcg gtctgcgaca ggaccagatt gtcaagtctt acttcctcgc cgccattgcc 1560

atctacgagc ccgacatggc ctccgcccga ctcgcctggg ccaagtctgc tgttctcatg 1620

gctgccatcc gaatcttctt ctccggtgag aactgctttg cccaccaccg acgacagttc 1680

ctcgacgcct tcacccgatg ggacggccga gccatgcgag actcccccaa ctccgccaag 1740

cgactcttct cttgtctctt ccgaatggtc aacctcttct ccgtcgacgg tgttgttgcc 1800

cagggccgag acatctccgg tgatctgcga caccgatggg agcactggct cgcctccgag 1860

gccgaggatc tgaccgacgc ccaggaccac gagaagctcg gtaccgaggc tgagattgtt 1920

gttctgaccg ctgctttcct cggccgagag actatctccc ccgatctcat ctcccacccc 1980

gacttctcct ccatcatgaa ggtcaccaac accgtctgct ctctgctgcg acgaattgcc 2040

acctacaagg aagagggctg cgactccccc tccggtactg aagaggacga ccgactcaag 2100

cgacgagctg aagagggtat gggccacctt gtccgagccg tctaccgaca ccagtactcc 2160

cccgtcccct ctggtgtcaa gcgactctgt ctggttgtcg gcaagtcttt ctactacgcc 2220

gcccactgca acaacgagga agtcggcaac cacgttgaga ctgttctctt ccagcccgtg 2280

tat 2283

<210> 154

<211> 1548

<212> DNA

<213> Bradyrhizobium japonicum

<400> 154

atgaacgccc tctccgagca cattctgtcc gagctccgac gactcctctc cgagatgtcc 60

gacggtggtt ccgtcggccc ctccgtctac gacaccgccc aggctctgcg attccacggt 120

aacgtcaccg gccgacagga cgcctacgcc tggctcattg cccagcagca ggctgacggt 180

ggctggggtt ccgccgactt ccctctcttc cgacacgccc ccacctgggc tgctctgctc 240

gctctgcagc gagccgaccc cctccccggt gctgccgacg ccgtccagac cgccacccga 300

ttcctccagc gacagcccga cccctacgcc cacgccgtcc ccgaggacgc ccccattggt 360

gccgagctca tcctccccca gttctgcggt gaggctgctt ctctgctcgg tggtgttgcc 420

ttcccccgac accccgctct gctccccctc cgacaggcct gtctggtcaa gctcggtgcc 480

gtcgccatgc tcccctccgg ccaccctctg ctccactctt gggaggcctg gggtacttct 540

cccaccactg cctgccccga cgacgacggc tccatcggta tctctcccgc tgccaccgcc 600

gcctggcgag cccaggctgt cacccgaggc tccactcccc aggtcggccg agccgatgcc 660

tacctccaga tggcctctcg agccacccga tccggtatcg agggtgtttt ccccaacgtc 720

tggcccatca acgtctttga gccctgctgg tccctctaca ccctccacct cgccggcctc 780

ttcgcccacc ccgctctcgc cgaggctgtc cgagtcatcg tcgcccagct cgacgcccga 840

ctcggtgtcc acggtcttgg ccccgctctg cactttgctg ccgacgccga cgacaccgcc 900

gttgctctct gtgttctgca cctcgccggc cgagatcccg ccgtcgatgc tctgcgacac 960

tttgagattg gtgagctctt cgtcaccttc cccggtgagc gaaacgcctc cgtttccacc 1020

aacatccacg ccctccacgc cctccgactc ctcggcaagc ccgctgccgg tgcctccgcc 1080

tacgtcgagg ccaaccgaaa cccccacggt ctgtgggaca acgagaagtg gcacgtcagc 1140

tggctctacc ccaccgccca cgccgttgct gctctcgccc agggcaagcc ccagtggcga 1200

gatgagcgag ctctggctgc tctgctccag gcccagcgag atgacggtgg ctggggtgct 1260

ggccgaggct ccaccttcga ggagactgcc tacgccctct tcgccctcca cgtcatggac 1320

ggctctgaag aggccaccgg ccgacgacga atcgcccagg ttgttgcccg agctctcgag 1380

tggatgctcg cccgacacgc cgcccacggt ctgccccaga cccctctgtg gatcggtaag 1440

gagctctact gccccacccg agttgtccga gttgccgagc tcgccggtct gtggctcgct 1500

ctgcgatggg gccgacgagt cctcgccgag ggtgccggtg ctgcccca 1548

<210> 155

<211> 2364

<212> DNA

<213> Lactuca sativa

<400> 155

atgaacattg cccagatcac ctcttctgcc atgctcgtcc cctcttccca cattccccac 60

cgatcttggg tcgtcaactg ctgcatggtc cagtacaacc cctccggtct gcgaaccgct 120

tcttcccagg ctggccaggt caaccccact gtcatgaccc tcgatgtcac caaggagcga 180

atccgaaagc tcttcaacaa cgttgaggtt tccgtttcct cttacgacac cgcctgggtt 240

gccatggtcc cctctcccaa ctcccccaag tctccttgtt tccccgactg tctcaactgg 300

ctcctcgaca accagctcga cgacggctct tggggtctgc tcccccacca gtctcctctg 360

atcaaggaca ccctctcttc cactctggcc tgtgtccttg ctctcaagcg atggaacgtc 420

ggtaaggacc agatcaacaa gggtctgcac tacattgagt ccaactttgc ctccgtcacc 480

gacaagaacc aggcttctcc ctttggcttc gacatcatct tccccggtat gctcgagtac 540

gccaaggacc tcgatatcaa gctgcccctc aaccagaccc acctgtccgt catgctccac 600

gagcgagagc tcgagctccg acgatgccac tccaacggcc gagaggccta cctcgcctac 660

atctccgagg gtcttggtaa cctcaacgac tggaacatgg tcatgaagta ccagatgaag 720

aacggctctc tgttcaactc cccctctgcc accgcctccg ttctcatcca ccaccagaac 780

gccggctgtc tgcactacct gacctctctg ctcgacaagt ttggtaacgc tgtccccacc 840

gtctacccca ttgatctgta cgtccgactc tccatggtcg acaccctcga gcgactcggt 900

atcaagcgac acttcatggt cgagatccag aacgttctcg acgagactta ccgatgctgg 960

gtccagggtg atgtccagat cttcatggac gttgtcacct gtgctctggc cttccgagtt 1020

ctgcgatcca acggttacga ggtttcttcc gaccctctgg ccaagatcac caaggaaggt 1080

gactacatga actcccccga gaagcccttc aaggacgtct acacctctct cgaggtctac 1140

aaggcctccc agatcatcta ccaagaggag ctcgccttcc gagagcagaa cctgacctcc 1200

tacctgccct cttccaacaa gctctccaac tacattctca aagaggtcga cgacgctctc 1260

aagttcccct tcaacggctc tctcgagcga atgtccaccc gacgaaacat tgagcactac 1320

aacctcaacc acacccgaat tctcaagacc acctactcct cttccaacat ctccaacaag 1380

gactacctca agctcgccgt ccaggacttc aacgagtgcc agtccatcta ctgtgaggag 1440

ctcaaggacc tcgagcgatg ggttgtcgag aaccgactcg acaagctcaa gtttgcccga 1500

caaaagactg cttactgcta cttctccgct gcctctttcc tctcctcccc cgatctgtcc 1560

gacgcccgaa tctcttgggc caagtcctcc attctgacca ccgtcattga cgacttcttc 1620

gatgtcggtg gctccatgga cgagctcgtc aactttgtcc acatcattga gaagtggaac 1680

gtcaacgtcg agaacgactg ctgctccgaa gaggtcggtg ttctcttcct ggctctcaag 1740

gacgccgtct gctggatcgg tgacaaggcc ttcaagatcc aggagcgaaa catcacctcc 1800

cacgtcattg agatctggct cgatctcgtc aagtccatgc tgcgagaggc catctgggcc 1860

aaggacggct ccatccccac catcaacgag tacatggaga acggctacgt ttcctttgct 1920

ctcggtccca ttgttctgcc cactctctac ttcctcggtg tcaagctgtc cgaggaagtt 1980

gtccagtcct ccgagtacca caagctctac gaggtcatgt ccacccaggg ccgactcatg 2040

aacgacatcc actccttcaa gcgagagaag aaggctggta agctcaacgc cgtcgccctc 2100

tacatgtccg acggcaagtc cggctccgtc gaagaggaag ttgtcgagga gatgaagatc 2160

ctcaccaagt cccagcgaaa ggagatgatg aagctcgttc tcgagactaa gggctccgtc 2220

gtcccccgag tctgcaagga tgttttctgg aacatgtgca acgttctcaa cctcttctac 2280

gccaccgacg acggtttcac tggtaacgcc attctcgatg ttgtcaagga gatcatctac 2340

gagcccgttt cccacgagct catc 2364

<210> 156

<211> 2250

<212> DNA

<213> Lactuca sativa

<400> 156

atggcttctt cccaggctgg ccaggtcaac cccactgtca tgaccctcga tgtcaccaag 60

gagcgaatcc gaaagctctt caacaacgtt gaggtttccg tttcctctta cgacaccgcc 120

tgggttgcca tggtcccctc tcccaactcc cccaagtctc cttgtttccc cgactgtctc 180

aactggctcc tcgacaacca gctcgacgac ggctcttggg gtctgctccc ccaccagtct 240

cctctgatca aggacaccct ctcttccact ctggcctgtg tccttgctct caagcgatgg 300

aacgtcggta aggaccagat caacaagggt ctgcactaca ttgagtccaa ctttgcctcc 360

gtcaccgaca agaaccaggc ttctcccttt ggcttcgaca tcatcttccc cggtatgctc 420

gagtacgcca aggacctcga tatcaagctg cccctcaacc agacccacct gtccgtcatg 480

ctccacgagc gagagctcga gctccgacga tgccactcca acggccgaga ggcctacctc 540

gcctacatct ccgagggtct tggtaacctc aacgactgga acatggtcat gaagtaccag 600

atgaagaacg gctctctgtt caactccccc tctgccaccg cctccgttct catccaccac 660

cagaacgccg gctgtctgca ctacctgacc tctctgctcg acaagtttgg taacgctgtc 720

cccaccgtct accccattga tctgtacgtc cgactctcca tggtcgacac cctcgagcga 780

ctcggtatca agcgacactt catggtcgag atccagaacg ttctcgacga gacttaccga 840

tgctgggtcc agggtgatgt ccagatcttc atggacgttg tcacctgtgc tctggccttc 900

cgagttctgc gatccaacgg ttacgaggtt tcttccgacc ctctggccaa gatcaccaag 960

gaaggtgact acatgaactc ccccgagaag cccttcaagg acgtctacac ctctctcgag 1020

gtctacaagg cctcccagat catctaccaa gaggagctcg ccttccgaga gcagaacctg 1080

acctcctacc tgccctcttc caacaagctc tccaactaca ttctcaaaga ggtcgacgac 1140

gctctcaagt tccccttcaa cggctctctc gagcgaatgt ccacccgacg aaacattgag 1200

cactacaacc tcaaccacac ccgaattctc aagaccacct actcctcttc caacatctcc 1260

aacaaggact acctcaagct cgccgtccag gacttcaacg agtgccagtc catctactgt 1320

gaggagctca aggacctcga gcgatgggtt gtcgagaacc gactcgacaa gctcaagttt 1380

gcccgacaaa agactgctta ctgctacttc tccgctgcct ctttcctctc ctcccccgat 1440

ctgtccgacg cccgaatctc ttgggccaag tcctccattc tgaccaccgt cattgacgac 1500

ttcttcgatg tcggtggctc catggacgag ctcgtcaact ttgtccacat cattgagaag 1560

tggaacgtca acgtcgagaa cgactgctgc tccgaagagg tcggtgttct cttcctggct 1620

ctcaaggacg ccgtctgctg gatcggtgac aaggccttca agatccagga gcgaaacatc 1680

acctcccacg tcattgagat ctggctcgat ctcgtcaagt ccatgctgcg agaggccatc 1740

tgggccaagg acggctccat ccccaccatc aacgagtaca tggagaacgg ctacgtttcc 1800

tttgctctcg gtcccattgt tctgcccact ctctacttcc tcggtgtcaa gctgtccgag 1860

gaagttgtcc agtcctccga gtaccacaag ctctacgagg tcatgtccac ccagggccga 1920

ctcatgaacg acatccactc cttcaagcga gagaagaagg ctggtaagct caacgccgtc 1980

gccctctaca tgtccgacgg caagtccggc tccgtcgaag aggaagttgt cgaggagatg 2040

aagatcctca ccaagtccca gcgaaaggag atgatgaagc tcgttctcga gactaagggc 2100

tccgtcgtcc cccgagtctg caaggatgtt ttctggaaca tgtgcaacgt tctcaacctc 2160

ttctacgcca ccgacgacgg tttcactggt aacgccattc tcgatgttgt caaggagatc 2220

atctacgagc ccgtttccca cgagctcatc 2250

<210> 157

<211> 2271

<212> DNA

<213> Picea glauca

<400> 157

atgaagcgag agcagtacac catcctcaac gagaaggagt ccatggccga ggagctcatc 60

ctccgaatca agcgaatgtt ctccgagatt gagaacaccc agacctccgc ctccgcctac 120

gacaccgcct gggttgccat ggtcccctct ctcgactcct cccagcagcc ccagttcccc 180

cagtgcctct cttggatcat cgacaaccag ctcctcgacg gctcttgggg tatcccctac 240

ctcatcatca aggaccgact ctgccacacc ctcgcctgtg tcattgctct gcgaaagtgg 300

aacgccggta accagaacgt cgagactggt ctgcgattcc tgcgagagaa cattgagggt 360

attgtccacg aggacgagta cacccccatt ggtttccaga tcatcttccc cgccatgctc 420

gaggaagctc gaggtctggg tctggagctc ccctacgatc tgacccccat caagctcatg 480

ctgacccacc gagagaagat catgaagggc aaggccattg accacatgca cgagtacgac 540

tcctctctca tctacaccgt cgagggtatc cacaagattg tcgactggaa caaggttctc 600

aagcaccaga acaaggacgg ctctctcttc aactccccct ctgccaccgc ctgtgctctc 660

atgcacaccc gaaagtccaa ctgtctggag tacctctctt ccatgctcca gaagctcggt 720

aacggtgtcc cctccgtcta ccccatcaac ctctacgccc gaatctccat gattgaccga 780

ctccagcgac tcggtctggc ccgacacttc cgaaacgaga tcatccacgc cctcgacgac 840

atctaccgat actggatgca gaaggagact tctcgagagg gcaagtctct cacccccgac 900

attgtttcca cctccattgc cttcatgctg ctgcgactcc acggctacga tgtccccgct 960

gacgttttct gctgctacca cctccactcc attgagcagt ccggtgaggc tgtcactgcc 1020

atgctgtctc tgtaccgagc ctcccagatc atgttccccg gtgagactat cctcgaggag 1080

atcaagaccg tttcccgaaa gtacctcgac aagcgaaagg agaacggtcg aatctactac 1140

cacaacattg tcatgaagga cctccgaggt gaggttgagt acgccctctc cgtcccctgg 1200

tacgcctctc tcgagcgaat cgagaaccga cgatacattg accagtacgg tgtcaacgac 1260

acctggatcg ccaagacctc ttacaagatc ccctgtatct ccaacgatct gttcctggct 1320

ctggccaagc aggactacaa catctgtcag gccatccagc agaaggagct ccgagagctc 1380

gagcgatggt tcgccgacaa caagttctcc cacctcaact ttgcccgaca gaagctcatc 1440

tactgctact tctccgctgc tgccactctc ttctctcccg agctctctgc tgctcgagtt 1500

gtctgggcca agaacggtgt catcaccacc gttgttgacg acttcttcga tgttggtggc 1560

tcctccgagg agatccactc ttttgtcgag gctgtccgag tctgggatga ggctgccacc 1620

gacggtctgt ccgagaacgt ccagatcctc ttctccgctc tgtacaacac cgttgacgag 1680

attgtccagc aggcctttgt ctttcagggc cgagacatct ccatccacct gcgagagatc 1740

tggtaccgac tcgtcaactc catgatgacc gaggcccagt gggcccgaac ccactgcatc 1800

ccctccatgc acgagtacat ggagaacgcc gagccttcca ttgctctcga gcccattgtt 1860

ctctcctctc tctactttgt cggccccaag ctgtccgagg agatcatctg ccaccccgag 1920

tactacaacc tcatgcacct gctcaacatc tgtggccgac tcctcaacga tatccagggc 1980

tgcaagcgag aggcccacca gggcaagctc aactccgtca ctctctacat ggaggagaac 2040

tccggcacca ccatggagga tgccattgtc tacctgcgaa agaccatcga cgagtcccga 2100

cagctcctcc tcaaagaggt tctgcgacct tccattgtcc cccgagagtg caagcagctc 2160

cactggaaca tgatgcgaat cctccagctc ttctacctca agaacgacgg tttcacctcc 2220

cccaccgaga tgctcggcta cgtcaacgcc gtcattgtcg accccatatt g 2271

<210> 158

<211> 900

<212> DNA

<213> Bradyrhizobium japonicum

<400> 158

atgatccaga ccgagcgagc cgtccagcag gttctcgagt ggggtcgatc tctgaccggc 60

tttgccgacg agcacgccgt cgaggccgtc cgaggtggtc agtacatcct ccagcgaatc 120

cacccctctc tgcgaggtac ctccgcccga accggccgag atccccagga cgagactctc 180

attgtcacct tctaccgaga gctcgctctg ctcttctggc tcgacgactg caacgatctc 240

ggcctcatct cccccgagca gctcgccgcc gtcgagcagg ctctcggcca gggtgtcccc 300

tgtgctctgc ccggtttcga gggctgtgcc gtcctccgag cttctctcgc caccctcgcc 360

tacgaccgac gagactacgc ccagctcctc gacgacaccc gatgctactc cgctgctctg 420

cgagctggtc acgcccaggc tgttgctgcc gagcgatggt cctacgccga gtacctccac 480

aacggtatcg actccattgc ctacgccaac gttttctgct gtctgtctct gctctggggc 540

ctcgacatgg ccactctgcg agcccgaccc gccttccgac aggttctgcg actcatctcc 600

gccatcggtc gactccagaa cgatctccac ggctgcgaca aggaccgatc cgccggtgag 660

gctgacaacg ccgtcattct gctcctccag cgataccccg ccatgcccgt tgtcgagttc 720

ctcaacgacg agctcgccgg ccacacccga atgctgcacc gagtcatggc cgaggagcga 780

ttccccgctc cctggggtcc tctcattgag gccatggctg ccatccgagt ccagtactac 840

cgaacctcca cctcccgata ccgatccgat gccgtccgag gtggtcagcg agctcccgcc 900

<210> 159

<211> 2838

<212> DNA

<213> Phaeosphaeria sp

<400> 159

atgttcgcca agttcgacat gctcgaggaa gaggcccgag ctctggtccg aaaggtcggt 60

aacgccgtcg accccatcta cggtttctcc accacctcct gccagatcta cgacaccgcc 120

tgggccgcca tgatctccaa agaggagcac ggtgacaagg tctggctctt ccccgagtcc 180

ttcaagtacc tcctcgagaa gcagggtgag gacggctctt gggagcgaca cccccgatcc 240

aagaccgtcg gtgttctcaa caccgccgcc gcctgtctgg ctctcctccg acacgtcaag 300

aaccccctgc agctgcagga cattgctgcc caggacatcg agctccgaat ccagcgaggt 360

ctgcgatctc tcgaggagca gctcatcgcc tgggatgacg ttctggacac caaccacatt 420

ggtgtcgaga tgattgtccc cgctctgctc gactacctcc aggccgagga cgagaacgtc 480

gacttcgagt ttgagtccca ctctctgctc atgcagatgt acaaggagaa gatggcccga 540

ttctcccccg agtctctcta ccgagctcga ccctcttctg ctctgcacaa cctcgaggct 600

ctcatcggta agctcgattt cgacaaggtc ggccaccacc tctacaacgg ctccatgatg 660

gcctccccct cttccactgc cgccttcctc atgcacgcct ctccctggtc ccacgaggcc 720

gaggcctacc tccgacacgt ctttgaggct ggtaccggca agggctccgg tggtttcccc 780

ggtacctacc ccaccaccta ctttgagctc aactgggtcc tctccaccct catgaagtcc 840

ggcttcactc tctccgatct cgagtgcgac gagctctcct ccattgccaa caccatcgcc 900

gagggtttcg agtgcgacca cggtgtcatc ggtttcgctc cccgagccgt cgacgttgat 960

gacactgcca agggtctgct caccctcacc ctcctcggta tggacgaggg tgtctccccc 1020

gctcccatga ttgccatgtt cgaggccaag gaccacttcc tgaccttcct cggtgagcga 1080

gatccctcct tcacctccaa ctgccacgtt ctgctctctc tgctccaccg aaccgacctc 1140

ctccagtacc tcccccagat ccgaaagacc accaccttcc tctgtgaggc ctggtgggcc 1200

tgcgacggcc agatcaagga caagtggcac ctctcccacc tctaccccac catgctcatg 1260

gtccaggcct ttgccgagat cctcctcaag tccgccgagg gtgagcctct gcacgacgcc 1320

tttgacgccg ccactctgtc tcgagtctcc atctgtgttt tccaggcctg tctgcgaact 1380

ctgctcgccc agtcccagga cggctcttgg cacggccagc ccgaggcttc ttgctacgcc 1440

gtcctcaccc tcgccgagtc cggccgactt gttctgctcc aggctctgca gccccagatt 1500

gctgctgcca tggagaaggc tgctgatgtc atgcaggctg gtcgatggtc ttgttccgac 1560

cacgactgtg actggacctc caagaccgcc taccgagtcg acctcgttgc tgctgcttac 1620

cgactcgccg ccatgaaggc ctcttccaac ctcaccttca ccgtcgacga caacgtttcc 1680

aagcgatcca acggtttcca gcagctcgtc ggccgaaccg acctcttctc cggtgtcccc 1740

gcctgggagc tccaggcctc tttcctcgag tccgctctgt ttgtccctct gctccgaaac 1800

caccgactcg atgtctttga ccgagatgat atcaaggttt ccaaggacca ctacctcgat 1860

atgatcccct tcacctgggt cggctgcaac aaccgatctc gaacctacgt ttccacctcc 1920

ttcctcttcg acatgatgat catctccatg ctcggctacc agattgacga gttcttcgag 1980

gctgaggctg cccccgcctt cgcccagtgc atcggtcagc tccaccaggt tgttgacaag 2040

gtcgtcgacg aggtcattga cgaggttgtc gacaaggttg tcggtaaggt cgtcggtaag 2100

gtcgtcggca aggtcgtcga cgagcgagtc gactccccca cccacgaggc catcgccatc 2160

tgcaacatcg aggcttctct gcgacgattc gtcgaccacg ttctccacca ccagcacgtt 2220

ctgcacgcct cccagcagga gcaggatatc ctctggcgag agctccgagc cttcctgcac 2280

gcccacgttg tccagatggc cgacaactcc actctcgctc ctcccggccg aaccttcttt 2340

gactgggtcc gaaccactgc tgccgaccac gttgcctgtg cctactcctt cgccttcgcc 2400

tgctgcatca cctccgccac cattggccag ggccagtcca tgtttgccac cgtcaacgag 2460

ctctacctgg tccaggctgc tgcccgacac atgaccacca tgtgccgaat gtgcaacgac 2520

attggctccg tcgaccgaga cttcattgag gccaacatca actccgtcca cttccccgag 2580

ttctccactc tgtctctcgt tgctgacaag aagaaggctc tggcccgact cgccgcctac 2640

gagaagtctt gtctgaccca caccctcgac cagtttgaga acgaggttct ccagtctccc 2700

cgagtctcct ccgccgcctc cggtgacttc cgaacccgaa aggtcgccgt tgtccgattc 2760

ttcgccgatg tcactgactt ctacgaccag ctctacatcc tccgagatct gtcctcctct 2820

ctcaagcacg tcgggaca 2838

<210> 160

<211> 2856

<212> DNA

<213> Gibberella fujikuroi

<400> 160

atgcccggca agattgagaa cggtaccccc aaggacctca agaccggcaa cgacttcgtt 60

tccgctgcca agtctctgct cgaccgagcc ttcaagtccc accactccta ctacggtctg 120

tgctccactt cctgccaggt ctacgacacc gcctgggttg ccatgatccc caagacccga 180

gacaacgtca agcagtggct cttccccgag tgtttccact acctgctcaa gacccaggct 240

gctgacggct cttggggctc tctgcccacc acccagaccg ccggtatcct cgacactgcc 300

tccgccgttc tggctctgct ctgccacgcc caggagcctc tgcagatcct cgatgtctcc 360

cccgatgaga tgggtctgcg aatcgagcac ggtgtcactt ctctcaagcg acagctcgcc 420

gtctggaacg acgttgagga caccaaccac attggtgtcg agttcatcat ccccgctctg 480

ctctccatgc tcgagaagga gctcgatgtc ccctccttcg agttcccttg ccgatccatt 540

ctggagcgaa tgcacggcga gaagctcggt cactttgatc tcgagcaggt ctacggcaag 600

ccctcttctc tgctgcactc tctcgaggcc ttcctcggca agctcgattt cgaccgactc 660

tcccaccacc tctaccacgg ctccatgatg gcctccccct cttccaccgc cgcctacctc 720

attggtgcca ccaagtggga cgacgaggcc gaggactacc tccgacacgt catgcgaaac 780

ggtgccggcc acggtaacgg tggtatctct ggtaccttcc ccaccaccca ctttgagtgc 840

tcctggatca ttgccactct cctcaaggtc ggtttcactc tcaagcagat tgacggtgac 900

ggtctgcgag gcctctccac cattctcctc gaggctctgc gtgacgagaa cggtgtcatt 960

ggcttcgctc cccgaaccgc cgacgttgat gacactgcca aggctctgct ggctctgtct 1020

ctggtcaacc agcccgtttc ccccgacatc atgatcaagg ttttcgaggg taaggaccac 1080

ttcaccacct ttggctccga gcgagatccc tctctgacct ccaacctcca cgttctcctc 1140

tctctgctca agcagtccaa cctgtctcag taccaccccc agatcctcaa gaccactctc 1200

ttcacctgtc gatggtggtg gggctccgac cactgtgtca aggacaagtg gaacctctcc 1260

cacctctacc ccaccatgct cctcgtcgag gccttcaccg aggttctgca cctcatcgac 1320

ggtggtgagc tctcctctct gtttgacgag tctttcaagt gcaagatcgg tctgtccatc 1380

ttccaggccg ttctccgaat catcctcacc caggacaacg acggctcttg gcgaggctac 1440

cgagagcaga cctgttacgc cattctcgcc ctcgtccagg cccgacacgt ctgtttcttc 1500

acccacatgg tcgaccgact ccagtcctgt gtcgaccgag gtttctcttg gctcaagtct 1560

tgctccttcc actctcagga cctcacctgg acctccaaga ccgcttacga ggttggtttc 1620

gttgccgagg cctacaagct cgctgctctc cagtccgcct ctctcgaggt ccccgctgcc 1680

accatcggcc actccgtcac ctctgctgtc ccctcctccg atctcgagaa gtacatgcga 1740

ctcgtccgaa agaccgctct gttctctccc ctcgatgagt ggggtctgat ggcctccatc 1800

atcgagtcct ccttctttgt ccccctcctc caggcccagc gagttgagat ctacccccga 1860

gacaacatca aggtcgacga ggacaagtac ctctccatca tccccttcac ctgggttggc 1920

tgcaacaacc gatctcgaac ctttgcctcc aaccgatggc tctacgacat gatgtacctg 1980

tctctgctcg gctaccagac cgacgagtac atggaggccg ttgccggccc cgtctttggt 2040

gatgtctctc tgctgcacca gaccatcgac aaggtcattg acaacaccat gggtaacctg 2100

gcccgagcca acggtaccgt ccactccggc aacggtcacc agcacgagtc ccccaacatt 2160

ggccaggtcg aggacaccct cactcgattt accaactccg tcctcaacca caaggacgtc 2220

ctcaactcct cctcctccga ccaggacacc ctccgacgag agttccgaac cttcatgcac 2280

gcccacatca cccagatcga ggacaactcc cgattctcca agcaggcttc ttccgacgcc 2340

ttctcctccc ccgagcagtc ctacttccag tgggtcaact ccactggtgg ttcccacgtt 2400

gcctgcgcct actctttcgc cttctccaac tgcctcatgt ccgccaacct cctccagggt 2460

aaggacgcct tcccttccgg tacccagaag tacctcatct cctccgtcat gcgacacgcc 2520

accaacatgt gccgaatgta caacgatttc ggttccatcg cccgagacaa cgccgagcga 2580

aacgtcaact ccatccactt tcccgagttc actctctgta acggtacctc ccagaacctc 2640

gacgagcgaa aggagcgact cctcaagatt gctacttacg agcagggcta cctcgaccga 2700

gctctcgagg ctctggagcg acagtcccga gatgatgctg gtgaccgagc cggctccaag 2760

gacatgcgaa agctcaagat tgtcaagctc ttctgtgacg tcaccgacct ctacgaccag 2820

ctctacgtca tcaaggacct ctcctcctcc atgaaa 2856

<210> 161

<211> 1533

<212> DNA

<213> Lactuca sativa

<400> 161

atggatctgc agaccatggc ccccatgggc tctgctgcca ttgccatcgg tggtcccgct 60

gttgccgttg ctggtggtat ctctctgctc ttcctcaagt ctttcctgtc ccagcagccc 120

ggcaacccca accacctccc ctccgtcccc gctgtccccg gtgtccctct gctcggtaac 180

ctgctcgagc tcaaggagaa gaagccctac aagaccttca ccaagtgggc cgagacttac 240

ggccccatct actccatcaa gactggtgcc acctccatgg tcgttgtcaa ctccaaccag 300

ctcgccaaag aggccatggt cacccgattc gactccatct ccacccgaaa gctctccaag 360

gctctccaga ttctcactgc tgacaagacc atggttgcca tgtccgacta cgacgactac 420

cacaagaccg tcaagcgaaa cctgctcacc tccatcctcg gtcccgctgc ccagaagcga 480

caccgagccc accgagatgc catgggtgac aacctgtctc gacagctcca cgctcttgct 540

ctcaactctc ctcaagaggc catcaacttc cgacagatct tccagtccga gctcttcact 600

ctcgccttca agcagacctt tggccgagac attgagtcca tcttcgtcgg tgatctcggt 660

accaccatga cccgagagga gatgttccag atcctcgttg tcgaccccat gatgggtgcc 720

attgacgttg actggcgaga cttcttcccc tacctcaagt ggatccccaa cgccaagctc 780

gaggagaaga ttgagcagat gtacatccga cgaaaggccg tcatgaaggc cgtcatccag 840

gagcaccgaa agcgaattga ctccggcgag aacctcgact cttacattga cttcctgctc 900

gccgaggccc agcctctgac cgagaagcag ctcctcatgt ctctgtggga gcccatcatt 960

gagacttccg acaccaccat ggtcaccacc gagtgggcca tgtacgagct ctccaagcac 1020

cccaacaagc agcagcgact ctacaacgag atccgaaaca tctgtggttc cgagaagatc 1080

accgaggaga agctctgcaa gatgccctac ctgtctgctg ttttccacga gactctgcga 1140

gtccactccc ccgtttccat catccccctg cgatacgtcc acgagaacac cgagctcggc 1200

ggctaccacg tccccgccgg caccgagctc gccgtcaaca tctacggctg caacatggag 1260

cgagagatct gggagaaccc cgaggagtgg tcccccgagc gattcctcgc cgagaacgag 1320

cccgtcaacc tgcagaaaac catggccttt ggtgctggta agcgagtctg cgctggtgcc 1380

atgcaggcca tgctgctcgc ctgtgtcggt attggccgaa tggtccagga gtttgagtgg 1440

cgactcaagg acgacgttga agaggatgtc aacaccctcg gcctcaccac ccagcgactc 1500

aaccccatgc tcgccgtcat caagccccgg aat 1533

<210> 162

<211> 1536

<212> DNA

<213> Lactuca sativa

<400> 162

atggacggtg tcattgacat gcagaccatc cccctgcgaa ctgccattgc cattggtggt 60

actgctgttg ctctcgttgt tgctctctac ttctggttcc tgcgatctta cgcctccccc 120

tctcaccact ccaaccacct tcctcccgtc cccgaggtcc ccggtgtccc cgtcctcggt 180

aacctgctcc agctcaagga gaagaagccc tacatgacct tcaccaagtg ggccgagatg 240

tacggcccca tctactccat ccgaaccggt gccacctcca tggttgttgt ctcttccaac 300

gagattgcca aggaagttgt tgtcacccga ttcccctcca tctccacccg aaagctctct 360

tacgctctca aggtcctcac tgaggacaag tccatggttg ccatgtccga ctaccacgac 420

taccacaaga ccgtcaagcg acacattctc accgccgtcc tcggccccaa cgcccagaag 480

aagttccgag cccaccgaga caccatgatg gagaacgttt ccaacgagct ccacgccttc 540

ttcgagaaga accccaacca ggaagtcaac ctgcgaaaga tcttccagtc tcagctcttt 600

ggtctggcca tgaagcaggc tctcggcaag gatgtcgagt ccatctacgt caaggatctc 660

gagactacca tgaagcgaga ggagatcttc gaggttctgg tcgtcgaccc catgatgggt 720

gccattgagg tcgactggcg agacttcttc ccctacctca agtgggtccc caacaagtct 780

ttcgagaaca tcatccaccg aatgtacacc cgacgagagg ctgtcatgaa ggctctcatc 840

caggagcaca agaagcgaat tgcctccggt gagaacctca actcctacat tgactacctg 900

ctctccgagg cccagaccct caccgacaag cagctcctca tgtctctgtg ggagcccatc 960

atcgagtcct ccgacaccac catggtcacc accgagtggg ccatgtacga gctcgccaag 1020

aaccccaaca tgcaggaccg actctacgag gagatccagt ctgtctgtgg ctccgagaag 1080

atcactgagg agaacctgtc ccagctcccc tacctctacg ccgttttcca ggagactctg 1140

cgaaagcact gccccgtccc catcatgccc ctgcgatacg tccacgagaa caccgtcctc 1200

ggtggctacc acgtccccgc tggtaccgag gttgccatca acatctacgg ctgcaacatg 1260

gacaagaagg tctgggagaa ccccgaggag tggaaccccg agcgattcct gtccgagaag 1320

gagtccatgg acctctacaa gaccatggcc tttggtggtg gcaagcgagt ctgtgccggc 1380

tctctgcagg ccatggtcat ctcctgcatt ggtatcggtc gactcgtcca ggactttgag 1440

tggaagctca aggacgatgc tgaggaagat gtcaacaccc tcggcctcac cacccagaag 1500

ctccaccctc tgctcgccct catcaacccc cggaaa 1536

<210> 163

<211> 1575

<212> DNA

<213> Sphaceloma manihoticola

<400> 163

atgatggacg acaccacctc cccctactcc acctaccact ccgtccgatc catccgaaac 60

cagtccgcct gggctctggc ccccattgcc gtctttatct gctacgttgt tctgcgacac 120

aaccgaaagt ccgtccccgc tgcctctgct ggttcccact ccatcctcga gcccctgtgg 180

ctcgcccgac tccgattcat ccgagactct cgattcatca tcggtcaggg ctactccaag 240

ttcaaggaca ccatcttcaa ggtcaccaag gtcggtgccg atatcattgt tgttgctccc 300

aagtacgtcg aggagatccg acgactctcc cgagacaccg gccgatccgt cgagcccttc 360

atccacgact ttgccggtga gctcctcggt ggcctcaact tcctcgagtc cgatctccag 420

acccgagttg tccagcagaa gctcaccccc aacctcaaga ccattgtccc cgtcatggag 480

gacgagatgc actacgctct cgtttccgag ctcgactctt gtctcgatgg ttccgagcac 540

tggacccgag tcgacatgat ccacatgctc tcccgaattg tctctcgaat ctccgcccga 600

atcttcctcg gccccaagta ctgccgaaac gatctgtggc tcaagaccac tgccgagtac 660

accgagaacc tcttcctgac cggtactctg ctccgattcg tcccccgaat gctccagaag 720

tggatcgctc ctctgctccc ctctttccga cagctccagg agaaccgaca ggccgcccga 780

aagatcatct ccgagattct gaccgaccac cagcccgaga agcacgacga gacttccgac 840

aacggtgacc cctaccccga cattctcacc ctcatgttcc aggctgcccg aggtaaggag 900

aaggacattg aggacattgc ccagcacact ctgctcctct ctctgtcctc catccacacc 960

actgctctga ccatgaccca ggctctctac gatctctgtg cctaccccca gtacctcgac 1020

cccgtcaagc acgagattgc cgacactctg cagtccgagg gctcttggtc caaggccatg 1080

ctcgacaagc tccacatgat ggactctctg ctccgagagt cccagcgact ctctcccgtc 1140

tttctgctca ccttcaaccg aatcctccac acccctctga ccctctccaa cggtatccac 1200

ctccccaagg gtacccgaat tgccgccccc tccgacgcca tcctcaacga cccctctctg 1260

gtccccggcc cccagcccgc tgacaccttc gaccccttcc gatacatcaa ccactccacc 1320

ggtgatgcca agaaaaccaa gaccaacttc cagaccacct ctctgcagaa catggccttt 1380

ggctacggta agtacgcctg ccccggccga ttctacgttg ccaacgagat caagctcgtt 1440

ctcggccacc tgctcatgca ctacgagttc aagttccctc ccggtatggg ccgacccgtc 1500

aactccactg tcgacaccga catgtacccc gatctcggtg cccgactcct cgtccgaaag 1560

cgaaagatgg aggag 1575

<210> 164

<211> 1440

<212> DNA

<213> Artemisia annua

<400> 164

atgcccatga ccgtcatgct gctctttgtt ttcctgctct tcattgccat ctgtttcttc 60

ctcgtccacc gacacaactc caccaccacc aagaacctgc ctcccggctc cttcggctgg 120

cccttcattg gtgagactct cgcctacatc cgatccaagc gaggtggtga ccccgagcga 180

ttcaccaagg agcgaatcga gaagtacggc tccactctgg tctttaagac ctccgttgcc 240

ggtgagcgaa tggccgtttt ctgcggtccc gagggtaaca agttcctctt tggcaacgag 300

aacaagctcg ttgcttcttg gtggcccaac tccgtccgaa ttctcttcga gaagtgtctg 360

atcaccatcc gaggtgacga ggccaagtgg ctccgaaaga tgatgttcgc ctacctcggt 420

cccgatgctc tgtccaaccg atacaccggt accatggagg ttgtcacccg actccacatc 480

cagaaccact ggcagggcaa gtccgagctc aaggtctttg agactgtccg accttacctc 540

ttcgagctcg cctgccgact cttcctgtct ctcgacgacc ccaagcacgt tgccgagctc 600

ggtaccctct tcaacacctt cctcaagggt ctgaccgagc tccccatcaa catccccggt 660

acccgattct accgagccaa gcgagctgcc aacgccatca agaagcagct cattgtcatc 720

atcaagcagc gacgacaggc tctcaagcaa gaggaccagt cctcctcctt cgaggacctc 780

ctctcccacc tccttgtctc ctccgacgag aacggccgat tcctgtccga ggctgagatt 840

gccaacaacg ttctgctcct cctcttcgcc ggccacgaca cttctgctgt ctccatcact 900

ctgctcatga agtctctggc cgagcacccc caggtctacg acaacgtcct caaggagcag 960

ctcggtattc tcgaggccaa ggctcccggt gagatgctca actgggagga tatccagaag 1020

atgcgatact cttggtacgt tgtctgcgag gtcatgcgac tcattcctcc cgttgtcggc 1080

tctttccgag aggctctggt cgactttgag tacgccggct acaccatccc caagggctgg 1140

aagatcatct ggtccgccgt catgacccac aaagaggaga acaacttccc caacgccacc 1200

aagttcgacc cctctcgatt cgagggtgct ggtcccaccc ccttcaccta cgtccccttc 1260

ggtggtggcc cccgaatgtg cctcggcaag gagctcgccc gagtccgaat cctcgtcttt 1320

ctgcacaaca tcatgaccaa gttcaagtgg gatctgctca tccccgacga gaagattggc 1380

tacgaccccc tcgccacccc cgtcaagggt ctgcccgtcc gactccaccc ccaccaggtt 1440

<210> 165

<211> 1365

<212> DNA

<213> Ricinus communis

<400> 165

atggagctcg tcatgttccc cgttctggct ctcgtttcca ctctcttcct gctcgccctc 60

cacttcatca tccgaaccct caaggagcga ctctttggct ctcccaacct gcctcccggc 120

cgactcggct ggcccctcat tggtgagact cccgccttct tccgagctgg ctttgaggcc 180

aagcccgaga agttcatcgg tgagcgaatg gagaagtacg actcccgagt ctttaagacc 240

tctctgctcg gcaagccctt cgccgtcatc tccggtaccg ccggccacaa gttcctcttc 300

tccaacgaga acaagctcgt caacctgtgg tggcccgagt ccgtccgaat gctcttcaag 360

tctgctctcg tttccgttgt cggtgacgag gccaagcgaa tccgaaagat gctcatgacc 420

ttcctcggcc tcgatgctct caagaactac accgagcgaa ttgacatggt cacccagcag 480

cacatccgaa cctactggga gggtaaagag gaagtcaccg tctactccac tctcaagctc 540

tacaccttca ctctggcctg caacctcttc gcctccatca acgaccccga gcgactctcc 600

aagctcggtg cccacttcga cgtctttgtc aagggtgtca tctctctgcc catctccatc 660

cccggtaccc gactctacaa gtccatgaag gctgccaacg ccatccgaga ggagctcaag 720

ctcattgtcc gagatcgaaa agaggctctc gagcgaaaga tggcctcccc cacccaggat 780

ctgctctcct acctgctcgt cgactccgac accaacggcc gattcctgtc cgagatggag 840

attctcgaca acatcatgct gctcctctac gctgagcagc tcgagattgc caactccaag 900

aagcccggtg agctgctcca gtgggaggac gtccagaaga tgcgatactc ttggaacgtc 960

atctccgagg ttctgcgact gtctcctccc gtttcttctg cctaccgaca cgccattgtt 1020

gacttcacct acgagggtta caccatcccc aagggctggc agctcttcac ctccttcggt 1080

accacccacc gagatcccgc tctcttcccc aaccccgagc gattcgacgc ctctcgattc 1140

gagggtaacg gtcctccctc ctactcctac atccccttcg gtggtggtcc ccgaatgtgc 1200

attggctacg agtttgcccg actcgagatg ctcatcttcc tgcacaacat catcaagcga 1260

ttcaagtggg acattctcat ccccgacgag cagtttggct acaaccccct gctcgccccc 1320

tcccagggtt tccccgtccg actgcgaccc caccactccc atctc 1365

<210> 166

<211> 1428

<212> DNA

<213> Stevia rebaudiana

<400> 166

atgatccagg ttctgacccc catcctcctc ttcctcatct tcttcgtttt ctggaaggtc 60

tacaagcacc aaaagaccaa gatcaacctg ccccctggct ccttcggctg gcccttcctc 120

ggtgagactc ttgctctgct ccgagccggc tgggactccg agcccgagcg attcgtccga 180

gagcgaatca agaagcacgg ctctcctctc gttttcaaga cctctctctt cggtgaccga 240

ttcgccgttc tctgtggtcc cgctggtaac aagttcctct tctgcaacga gaacaagctc 300

gttgcctctt ggtggcccgt ccccgtccga aagctcttcg gcaagtctct gctcaccatc 360

cgaggtgacg aggccaagtg gatgcgaaag atgctgctct cctacctcgg tcccgatgcc 420

tttgccaccc actacgctgt caccatggac gttgtcaccc gacgacacat tgacgtccac 480

tggcgaggca aggaagaggt caacgttttc cagaccgtca agctctacgc ctttgagctc 540

gcctgccgac tcttcatgaa cctcgacgac cccaaccaca ttgccaagct cggctctctc 600

ttcaacatct tcctcaaggg tatcatcgag ctccccattg acgttcccgg tacccgattc 660

tactcctcca agaaggctgc tgctgccatc cgaatcgagc tcaagaagct catcaaggcc 720

cgaaagctcg agctcaagga aggcaaggcc tcctcctccc aggatctgct gtcccacctg 780

ctcacctccc ccgacgagaa cggtatgttc ctgaccgagg aagagattgt cgacaacatt 840

ctgctcctcc tctttgccgg ccacgacacc tctgctctgt ccatcactct gctcatgaaa 900

actctcggtg agcactccga tgtctacgac aaggtcctca aggagcagct cgagatctcc 960

aagactaaag aggcctggga gtctctcaag tgggaggaca tccagaagat gaagtactcc 1020

tggtccgtca tctgcgaggt catgcgactc aaccctcccg tcatcggtac ctaccgagag 1080

gctctggtcg acattgacta cgccggctac accatcccca agggctggaa gctccactgg 1140

tccgctgtct ccacccagcg agatgaggcc aactttgagg acgtcacccg attcgacccc 1200

tctcgattcg agggtgccgg tcccactccc ttcacctttg tccccttcgg tggtggtccc 1260

cgaatgtgtc tcggcaagga gtttgcccga ctcgaggttc tggccttcct gcacaacatt 1320

gtcaccaact tcaagtggga tctgctcatc cccgacgaga agattgagta cgaccccatg 1380

gccacccccg ccaagggtct gcccatccga ctccaccccc accaggtg 1428

<210> 167

<211> 1575

<212> DNA

<213> Arabidopsis thaliana

<400> 167

atggagtctc tggttgtcca caccgtcaac gccatctggt gcattgtcat tgtcggtatc 60

ttctccgtcg gctaccacgt ctacggccga gctgttgtcg agcagtggcg aatgcgacga 120

tctctcaagc tccagggtgt caagggtcct cctccctcca tcttcaacgg taacgtttcc 180

gagatgcagc gaatccagtc cgaggccaag cactgctccg gtgacaacat catctcccac 240

gactactctt cttctctgtt cccccacttt gaccactggc gaaagcagta cggccgaatc 300

tacacctact ccactggcct caagcagcac ctctacatca accaccccga gatggtcaag 360

gagctctccc agaccaacac cctcaacctc ggccgaatca cccacatcac caagcgactc 420

aaccccattc tcggtaacgg tatcatcacc tccaacggcc cccactgggc ccaccagcga 480

cgaatcattg cctacgagtt cacccacgac aagatcaagg gtatggtcgg tctgatggtc 540

gagtccgcca tgcccatgct caacaagtgg gaggagatgg tcaagcgagg tggtgagatg 600

ggctgtgaca tccgagtcga cgaggacctc aaggatgtct ccgctgacgt cattgccaag 660

gcctgtttcg gctcttcctt ctccaagggc aaggccatct tctccatgat ccgagatctg 720

ctcaccgcca tcaccaagcg atccgtcctc ttccgattca acggtttcac cgacatggtt 780

ttcggctcca agaagcacgg tgacgttgac attgacgctc tcgagatgga gctcgagtcc 840

tccatctggg agactgtcaa ggagcgagag attgagtgca aggacaccca caagaaggac 900

ctcatgcagc tcattctcga gggtgccatg cgatcttgtg acggtaacct gtgggacaag 960

tctgcttacc gacgattcgt tgtcgacaac tgcaagtcca tctactttgc cggccacgac 1020

tccaccgccg tttccgtttc ttggtgcctc atgctgctcg ctctcaaccc ctcttggcag 1080

gtcaagatcc gagatgagat tctgtcctcc tgcaagaacg gtatccccga cgccgagtcc 1140

atccccaacc tcaagaccgt caccatggtc atccaggaga ctatgcgact ctaccctccc 1200

gctcccattg tcggccgaga ggcctccaag gacattcgac tcggtgatct ggttgtcccc 1260

aagggtgtct gtatctggac cctcatcccc gctctgcacc gagatcccga gatctggggt 1320

cccgacgcca acgacttcaa gcccgagcga ttctccgagg gtatctccaa ggcctgcaag 1380

tacccccagt cctacatccc ctttggcctc ggcccccgaa cctgtgtcgg caagaacttt 1440

ggtatgatgg aggtcaaggt cctcgtttct ctgattgtct ccaagttctc cttcactctg 1500

tctcccacct accagcactc tccctcccac aagctgctcg tcgagcccca gcacggtgtt 1560

gtcatccgag ttgta 1575

<210> 168

<211> 1437

<212> DNA

<213> Ixeris dentata

<400> 168

atggacgccg ttgccgtcaa ctccgagact atgtcccacg ttgtctttat ccccttcccc 60

gcccagtccc acatcaagtg catgctcaag ctcgcccgac tgctgcacca caagggtctg 120

cacatcacct ttgtcaacac cgagctcaac cacaaccagc tgctgtcctc cggtggtccc 180

aactctctcg acggtgagcc cggtttccga ttcaagacca tccccgatgg tgtccccgag 240

ggtgctcccg acttcatgta cgctctgtgt gactccgttc tcaacaagat gctcgacccc 300

ttcgtcgatc tcattggccg actcgagtcc cccgccacct gtatcatcgg tgacggtatg 360

atgcccttca ctgttgctgc tgccgagaag ctcaagctcc ccatcatgca cttctggacc 420

ttccccgctg ctgccttcct cggctactac caggccccca acctcattga gaagggcttc 480

atccctccca aggacgagtc ttggtccacc aacggctacc tcgagactgt tgtcgactcc 540

atctccggtc tggagggttt ccgaatccga gacatccccg cctacttccg aaccaccgac 600

cccaacgact ccgacttcaa ctacatcatt gagtgtgtca aggccatccg aaaggtttcc 660

aacattgttc tccacacctt tgaggagctc gagtccacca tcatcaaggc tctgcagccc 720

atgatccccc acgtctacac cattggcccc ctcgagctcc tcctcaaccc catcaagctc 780

gaagaggaga ctgagaagct cgatatcaag ggctactctc tgtggaagga agatgacgag 840

tgcctcaagt ggctcgactc caaggagccc aactccgtca tctacgtcaa cttcggctct 900

ctcatctcca tgtccaagga gcagctcgcc gagtttggct ggggtctggt caactccaac 960

cactgcttcc tctgggtcat ccgacgagat ctggttgtcg gtgactctgc tcctctgcct 1020

cccgagctca aggagcgaat caacgagcga ggtttcattg cctcttggtg cccccaggag 1080

aaggtcctca agcactcctc cgtcggtggt ttcctcaccc actgtggctg gggctccatc 1140

atcgagtctc tgtccgccgg tgtccccatg ctctgctggc cctacctctg ggaccagccc 1200

accaactgcc gacaggcctg caaggagtgg gaggtcggtc tggagattga gggtaacgtc 1260

aacaaggacg aggtcgagcg actcacccga gagctcattg gtggtgagaa gggcaagcag 1320

atgcgatcca aggccctcga gtggaagaag aagattgaga ttgccaccgg ccccaagggc 1380

tcttcttctc tcaacgtcga gcgactggcc aacgacatca acatgttctc ccgaaat 1437

<210> 169

<211> 1446

<212> DNA

<213> Ricinus communis

<400> 169

atgggctcca ttgtccgaga tcacgacaag ccccacgttg tctgtgtccc ctaccccgcc 60

cagggccacg tcaaccccat ggtcaagctc gccaagctcc tccactacaa cgacttccac 120

gtcacctttg tcaacaccga gtacaaccac cgacgactcc tcaactcccg aggcccctct 180

tctctcgacg gcctccccga cttccgattc gaggccatct ccgacggtct gcccccttcc 240

gacgccaacg ccacccagga tatcccctct ctctgtgact ccacctccaa gaactctctg 300

gcccccttcc gaaacctgct gctcaagctc aagtcctccg actctctgcc tcccgtcacc 360

tgtatcatct ccgacgcctg catgtccttc accctcgatg ctgctgagga gttcggtatc 420

cccgagatcc tcttctggac cccctcttct tgtggtgttc tcggctactc ccagtaccac 480

actctcattg agaagggtct gactcccctc aaggacgcct cctacctgac caacggctac 540

ctcgagacta ccctcgactg gatccccggt atgaaggaca tccgattccg agatctgccc 600

tctttcatcc gaaccaccga ccgaaacgac atcatgctca acttcgttgt ccgagagctc 660

gagcgaacct cccgagcctc tgctgttgtt ttcaacacct tctacgcctt tgagaaggac 720

gttctcgacg ttctgtccac catgttccct cccatctact ccatcggtcc cctccagctc 780

ctcgtcgacc agatccccat tgaccgaaac ctcggtaaca ttggctccaa cctgtggaag 840

gagcagcccg agtgcattga ctggctcgac accaaggagc ccaactccgt tgtctacgtc 900

aactttggct ccatcaccgt catcactccc cagcagatga ttgagtttgc ctggggtctg 960

gcctcttcca agaagccctt cctgtggatc atccgacccg atctggtcat tggtgagaac 1020

gccatgctcc ccgctgagtt tgtctccgag actaaggacc gaggtatgct cgcctcttgg 1080

ggtccccagg agcagatcct caagcacccc gctgtcggtg gtttcctgtc ccacatgggc 1140

tggaactcca ccctcgactc catgtccggt ggtgtcccca tggtctgctg gcccttcttc 1200

gccgagcagc agaccaactg ccgattcgcc tgcaccgagt ggggtgtcgg tatggagatt 1260

gacaacaacg tcaagcgaga tgaggtcaag aagctcgttg aggtcctcat ggacggcaag 1320

aagggcaagg agatgaagtc caaggccatg gagtggaaaa ccaaggctga ggaagctgcc 1380

aagcccggtg gttcctccca caacaacctc gaccgactcg tcaagttcat caagggccag 1440

aagaat 1446

<210> 170

<211> 1374

<212> DNA

<213> Ixeris dentata

<400> 170

atggccgagg agcacaacaa gaccaacaac tcctctcccc acgttgtcat cttccccttc 60

ccctcccagg gccacatcaa ccccctcatc cagtttgcca agcgactctc ctccaagggt 120

gtcaagccca ctctcatcac caccatctac attgccaaga cctctcctta ccccaactcc 180

tccattgttg tcgagcccat ctccgacggt ttcgacgacg gtggtttcaa gtccgccacc 240

tccgctgagt cctacattga caccttccac caggtcggct ccaagtctct ggccaacctc 300

atccgaaagc tcgtcaacga gggcaaccac gttgatgcca tcatctacga ctccttcgtc 360

acctgggctc tcgatgttgc catggagtac ggtattgacg gtggctgctt cttcacccag 420

gcctgtgccg tcaacaacat ctactaccac gtctacaagg gtgtcctcga gatccccctg 480

caggctgctg ctcctcccac cgtcaccatt ctgctccccg agctccccca gctccagctg 540

tgggagactc cctcttttgt ccacaacccc ggcccctacc ccggctgggc ccacattgtc 600

tttaaccagt tccccaacat ccacaacgcc cgatgggttt tctccaacac cttcttcaag 660

ctcgaggagc aggtcatcaa gtggatgcga ctcatgtggc ctctcatggt cgtcggtccc 720

accgtcccct ccatgtacct cgacaagcga ctcgaggacg acgacgacta cggtatgtct 780

ctgctcaagc ccaaccacat tgagtgcatg ggctggctca acaacaagcc caagggctcc 840

gtcgtctacg tttctttcgg ctcttacggt gagctcggtg ttgcccagat ggaggagatt 900

gcctggggtc tgaacgagtc ctccgtcaac tacctgtggg ttgtccgaga gactgagaag 960

gagaagctcc ccaagtcctt cctggccaac ggtctgattg tcgagtggtg ccgacagctc 1020

gaggtcctcg cccacgaggc tgttggctgt ttcgtcaccc actgcggttt caactcctct 1080

ctcgagacta tctctctcgg tgtccccgtt gttgccatcc cccagtggac cgaccagacc 1140

accaacgcca agtgcctcga ggatatctgg ggtgtcggta tccgagccaa gacccccgtc 1200

acccgaacca acctggtctg gtgcatcaag gagatcatgg agggtgagcg aggtgctgtt 1260

gctcgaaaga acgccatcaa gtggaaggat ctggccattg aggctgtctc ccccggcggc 1320

tcttccgaca aggacatcaa cgagtttgtc tcccagctct cccccatcaa gtgt 1374

<210> 171

<211> 1362

<212> DNA

<213> Populus trichocarpa

<400> 171

atggacaaca agaagtccca cgtcattgtt ctgacctacc ccgcccaggg ccacatcaac 60

cccctgctcc agtttgccaa gcgactcgcc tccaagggcc tcaaggccac tctggccacc 120

acctactaca ccgtcaactc catcgacgcc cccaccgtcg gtgttgagcc catctccgac 180

ggtttcgacg agggtggttt caagcaggct tcttctctcg atgtctacct cgagtccttc 240

aagaccgtcg gctcccgaac tctgaccgag ctcgttttca agttcaaggc ttccggctct 300

cccgtcaact gtgttgtcta cgactccatg ctcccctggg ccctcgacgt tgctcgagat 360

ctcggtatct acgctgctgc cttcatgacc acctctgctt ccgtctgctc catgtactgg 420

cgaatcgatc tcggtctgct gtctctgccc ctcaagcagc agaccgccac tgtctctctg 480

cccggtctgc cccctctcgg ctgctgtgac ctcccctctt tcctcgccga gcccacctcc 540

cagaccgcct acctcgaggt catcatggag aagttccact ctctcaacga ggacgactgg 600

gttttctgca actccttcga ggacctcgag attgagctcg tcaaggccat gcgaggcaag 660

tggcccctcg tcatggtcgg ccccatggtc ccctccgcct acctcgacca gcagatcgac 720

ggtgaccgag cctacggtgc ttctctgtgg aagcccacct cctcccagtg cttcacctgg 780

ctcgacacca agccccctcg atccgtcatc tacgtttcct tcggctccat gggcaacatc 840

tccgccgagc aggttgagga gattgcctgg ggcctcaagg cttccaaccg acccttcctg 900

tgggtcatga aggagtccga gaagaagctc cccaccggtt tcctcaactc cgtcggtgag 960

actggtatgg ttgtctcttg gtgcaaccag ctcgaggttc tcgcccacca ggccattggc 1020

tgctttgtca cccactgtgg ctggaactcc actctcgagg gtctgggcct cggtgtcccc 1080

atggtctgtg tcaccgagcg atccgaccag cccatgaacg ccaagttcgt cgaggatgtc 1140

tggaaggtcg gtgtccgagc caagaaggac gaggttggta ttgtcacccg agaggagctc 1200

gagaagtgca tccgaggtgt catggacggt gagaacggtg aggagatcaa gcgaaacgcc 1260

aacaagtggc gagagctcgc ccgatctgct gtttccgtcg gtggttcctc cgacatgaac 1320

atcaacgagt ttgttgtcaa gctgctcgag ggcaagaagg ga 1362

<210> 172

<211> 1377

<212> DNA

<213> Nicotiana tabacum

<400> 172

atgaccaccc agaaggccca ctgtctgatc ctcccctacc ccgcccaggg ccacatcaac 60

cccatgctcc agttctccaa gcgactccag tccaagggtg tcaagatcac cattgctgcc 120

accaagtctt tcctcaagac catgcaggag ctgtccacct ctgtctccgt cgaggccatc 180

tccgacggct acgacgacgg tggccgagag caggctggta cctttgtcgc ctacatcacc 240

cgattcaaag aggttggctc cgacactctg tcccagctca tcggtaagct caccaactgt 300

ggctgccctg tctcttgcat tgtctacgac cccttcctgc cctgggctgt cgaggtcggt 360

aacaacttcg gtgttgccac tgctgccttc ttcacccagt cttgtgccgt cgacaacatc 420

tactaccacg tccacaaggg tgttctcaag ctccctccca ccgacgttga caaggagatc 480

tccatccccg gtctgctcac cattgaggct tccgatgtcc cctcttttgt ctccaacccc 540

gagtcctccc gaatcctcga gatgctcgtc aaccagttct ccaacctcga gaacaccgac 600

tgggttctca tcaactcctt ctacgagctc gagaaggaag tcattgactg gatggccaag 660

atctacccca tcaagaccat tggccccacc atcccctcca tgtacctcga caagcgactc 720

cccgatgaca aggagtacgg tctgtccgtc tttaagccca tgaccaacgc ctgcctcaac 780

tggctcaacc accagcccgt ttcttccgtc gtctacgttt ctttcggctc tctggccaag 840

ctcgaggctg agcagatgga ggagctcgcc tggggtctgt ccaactccaa caagaacttc 900

ctgtgggttg tccgatccac tgaggagtcc aagctcccca acaacttcct cgaggagctc 960

gcctccgaga agggtctggt tgtctcttgg tgcccccagc tccaggttct cgagcacaag 1020

tccatcggtt gcttcctgac ccactgcggc tggaactcca ctctcgaggc catctctctc 1080

ggtgtcccca tgattgccat gccccactgg tccgaccagc ccaccaacgc caagctcgtc 1140

gaggatgtct gggagatggg tatccgaccc aagcaggatg agaagggtct ggtccgacga 1200

gaggtcattg aggagtgtat caagattgtc atggaggaga agaagggcaa gaagatccga 1260

gagaacgcca agaagtggaa ggagctcgcc cgaaaggctg ttgacgaggg tggctcttcc 1320

gaccgaaaca ttgaggagtt cgtttccaag ctcgtcacca tcgcttccgt cgaatcg 1377

<210> 173

<211> 1434

<212> DNA

<213> Vaccaria hispanica

<400> 173

atgtccaaca acgagaacaa cgccacccag gtcattgttc tgccctacca cggccagggc 60

cacatgaaca ccatggtcca gtttgccaag cgactcgcct ggaagggtgt ccacgtcacc 120

atcgccacca ccttcaacac catccagcag atgaagctca acatctcctc ttacaactcc 180

atcactctcg agcccatcta cgacgacacc gacgactcca ctctgcacat caaggaccga 240

atggcccgat tcgaggctga ggctgcctcc aacctgaccc gagttctcga ggccaagaag 300

cagcagcagg ctctcaacaa gaagtgtctg ctcgtctacc acggctctct caactgggcc 360

ctcgttgtcg cccaccagca gaacgttgcc ggtgctgcct tcttcaccgc cgcctctgct 420

tctttcgcct gctactacta cctgcacctc gagtcccagg gcaagggtgt cgatctcgag 480

gagctcccct ccattctgcc ccctcccaag gtcatcgtcc agaagctccc caagtccttc 540

ctggcctacg gtgacaacaa ctcccacaac aacaacaata acaacaataa caacaacaat 600

aacaacaaca tgggtctgca ccctctggtt ctgtggctcc tcaaggacta cggcaactcc 660

gtcaaggccg actttgtcct cctcaactcc ttcgacaagc tcgaggaaga ggccatcaag 720

tggatctcca acatctgctc cgtcaagacc atcggtccca ccatcccctc cacctacctc 780

gacaagcaga tcgagaacga tgtcgactac ggtttcaacc agtacaagcc caccaacgag 840

gactgcatga agtggctcga caccaaggaa gccaactccg ttgtctacat tgccttcggc 900

tctgttgctc gactgtccgt cgagcagatg gccgagattg ccaaggctct cgaccactcc 960

tccaagtctt tcatctgggt tgtccgagag actgagaagg agaagctccc cgtcgacctc 1020

gtcgagaaga tctccggcca gggtatggtt gtcccctggg ctccccagct cgaggtcctc 1080

gcccacgatg ctgtcggctg tttcgtttcc cactgtggct ggaactccac cattgaggct 1140

ctgtctttcg gtgtccccat cctcgccatg ccccagttcc tcgaccagct cgttgatgcc 1200

cactttgtcg accgagtctg gggtgtcggt attgccccca ctgtcgacga gaacgatctc 1260

gtcacccagg aagagatctc ccgatgcctc gacgagatga tgggtggtgg tcccgagggt 1320

gagaagatca agaagaacgt tgccatgtgg aaggagctga ccaaagaggc tctcgacaag 1380

ggtggctctt ccgacaagca cattgacgag atcattgagt ggctctcttc tagt 1434

<210> 174

<211> 1443

<212> DNA

<213> Streptococcus mutans

<400> 174

atgcccatca tcaacaagac catgctcatc acctacgccg actctctcgg caagaacctc 60

aaggagctca acgagaacat tgagaactac ttcggtgatg ctgttggtgg tgtccacctg 120

ctccccttct tcccctccac tggtgaccga ggcttcgccc ccattgacta ccacgaggtt 180

gactctgcct tcggtgactg ggatgatgtc aagtgcctcg gtgagaagta ctacctcatg 240

ttcgacttca tgatcaacca catctcccga cagtccaagt actacaagga ctaccaggag 300

aagcacgagg cttctgccta caaggacctc ttcctcaact gggacaagtt ctggcccaag 360

aaccgaccta cccaggaaga tgtcgatctc atctacaagc gaaaggaccg agctcccaag 420

caggagatcc agtttgccga cggctccgtc gagcacctgt ggaacacctt cggtgaggag 480

cagattgacc tcgatgtcac caaggaagtc accatggact tcatccgatc caccattgag 540

aacctggctg ccaacggctg tgacctcatc cgactcgacg cctttgccta cgccgtcaag 600

aagctcgaca ccaacgactt cttcgtcgag cccgagatct ggactctgct cgacaaggtc 660

cgagacattg ctgccgtttc cggtgccgag atcctccccg agatccacga gcactacacc 720

atccagttca agattgctga ccacgactac tacgtctacg actttgctct gcccatggtc 780

actctgtact ctctgtactc ctccaaggtc gaccgactcg ccaagtggct caagatgtct 840

cccatgaagc agttcaccac cctcgacacc cacgacggta tcggtgttgt tgatgtcaag 900

gacattctga ccgacgagga gatcacctac acctccaacg agctctacaa ggtcggtgcc 960

aacgtcaacc gaaagtactc cactgccgag tacaacaacc tcgatatcta ccagatcaac 1020

tccacctact actctgctct cggtgacgac gaccagaagt acttcctggc ccgactcatc 1080

caggcctttg cccccggtat cccccaggtc tactacgttg gcttcctcgc cggcaagaac 1140

gatctcgagc tgctcgagtc caccaaagag ggccgaaaca tcaaccgaca ctactactcc 1200

tccgaggaga ttgctaaaga ggtcaagcga cccgttgtca aggctctgct caacctcttc 1260

acctaccgaa accagtccgc cgcctttgac ctcgacggcc gaatcgaggt tgagactccc 1320

aacgaggcca ccattgtcat cgagcgacag aacaaggacg gctctcacat tgccaaggcc 1380

gagatcaacc tgcaggacat gacctaccga gtcaccgaga acgaccagac catctccttc 1440

gag 1443

<210> 175

<211> 1392

<212> DNA

<213> Lobelia erinus

<400> 175

atggacaaca accacctcgg tgagactctg ctccccctcg ctcccaagaa cggccgacga 60

gttctcttct tcccctaccc cctccagggc cacatctccc ccatgctcaa cctcgccaac 120

ctgctccact ccaagggttt caccatcacc atcatccaca ccaacctcaa ctcccccaac 180

cagtccgact acccccactt caccttccga ccctttgacg acggtttccc tccttactcc 240

aagggctggc agctcgccac cctctgctcc cgatgtgtcg agcccttccg agagtgtctg 300

gcccagatct tcctgtccga ccacaccgcc cccgagggtg agcgagagtc cattgcctgc 360

ctcattgccg atggtctgtg gaacttcctc ggtgctgccg tctacaactt caagctcccc 420

atgattgttc tgcgaaccgg taacatgtcc aacattgttg ccaacgtcaa gctcccctgc 480

ttcatcgaga agggctactt tgaccacacc aaggaaggct ccaagctcga ggctgccgtc 540

cccgagttcc ccaccatcaa gttcaaggac attctcaaga cctacggctc caaccccaag 600

gccatctgtg agactctgac cgctctgctc aaggagatgc gagcttcttc cggtgtcatc 660

tggaactcct gcaaggagct cgagcagtcc gagctccaga tgatctgcaa ggagttcccc 720

gtcccccact tcctcatcgg tcctctgcac aagtacttcc ccgcctcttc ttcttctctc 780

gttgcccacg acccctcttc catctcttgg ctcaactcca aggctcccaa ctccgtcctc 840

tacgtttctt ttggctccat ctcctccatg gacgaggctg agttcctcga gactgcctgg 900

ggtctggcca actccatgca gcagttcctg tgggttgtcc gacccggctc cgtccgaggt 960

tcccagtggc tcgagtctct gcccgatggt ttcattgaca agctcgacgg ccgaggccac 1020

attgtcaagt gggctcccca gcaagaggtt ctcgcccacc aggccaccgg tggtttctgg 1080

acccactgtg gctggaactc cactctcgag tccatgtgcg agggtgtccc catgatctgc 1140

tcccacggta tcatggacca gcccatcaac gcccgatacg tcaccgatgt ctggaaggtc 1200

ggtatcgagc tcgagaaggg ctttgactcc gaggagatca agatggccat ccgacgactc 1260

atggtcgata aagagggcca ggagatccga gagcgatctt ctcgactcaa ggagtctctg 1320

tccaactgtc tcaagcaggg tggttcctcc cacgactccg tcgagtctct ggtcgaccac 1380

attctcagct tc 1392

<210> 176

<211> 1380

<212> DNA

<213> Arabidopsis thaliana

<400> 176

atggaggagc gaaagggccg acgaatcatc atgttccctc tgcccttccc cggccacttc 60

aaccccatga tcgagctcgc tggtatcttc caccaccgag gcttctccgt caccatcctc 120

cacacctcct acaacttccc cgacccctct cgacaccccc acttcacctt ccgaaccatc 180

tcccacaaca aggaaggcga agaggacccc ctgtcccagt ccgagacttc ttccatggac 240

ctcattgttc tcgtccgacg actcaagcag cgatacgctg agcccttccg aaagtccgtt 300

gctgctgagg tcggtggtgg tgagactgtc tgctgcctcg tttccgatgc catctggggt 360

aagaacaccg aggttgttgc cgaggagatt ggtgtccgac gagttgtcct ccgaaccggt 420

ggtgcctctt ctttctgcgc ctttgctgct ttccctctgc tccgagacaa gggctacctc 480

cccatccagg actcccgact cgacgagccc gtcaccgagc tgccccctct caaggtcaag 540

gatctgcccg tcatggagac taacgagccc gaggagctct accgagttgt caacgacatg 600

gtcgagggtg ccaagtcctc ttccggtgtc atctggaaca cctttgagga tctcgagcga 660

ctctctctca tgaactgctc ctccaagctc caggtcccct tcttccccat cggtcccttc 720

cacaagtact ccgaggaccc cacccccaag accgagaaca aggaagatac cgactggctc 780

gacaagcagg acccccagtc cgttgtctac gcctcttttg gctctctcgc cgccatcgag 840

gagaaggagt tcctcgagat tgcctggggt ctgcgaaact ccgagcgacc cttcctgtgg 900

gttgtccgac ccggctccgt ccgaggcacc gagtggctcg agtctctgcc cctcggcttc 960

atggagaaca ttggtgacaa gggcaagatc gtcaagtggg ccaaccagct cgaggttctg 1020

gcccaccccg ccatcggtgc cttctggacc cactgtggct ggaactccac tctcgagtcc 1080

atctgtgagg gtgtccccat gatctgcacc tcttgtttca ccgaccagca cgtcaacgcc 1140

cgatacattg tcgatgtctg gcgagtcggt atgctgctcg agcgatccaa gatggagaag 1200

aaggagattg agaaggtcct ccgatccgtc atgatggaga agggtgacgg tctgcgagag 1260

cgatctctca agctcaagga gcgagccgac ttctgcctct ccaaggacgg ctcctcctcc 1320

aagtacctcg acaagcttgt ctcccacgtt ctgtcttttg actcctacgc ctttgcatcc 1380

<210> 177

<211> 2139

<212> DNA

<213> Gibberella fujikuroi

<400> 177

atggctgagc tcgacactct cgacattgtt gttctcggtg tcatcttcct cggtaccgtt 60

gcctacttca ccaagggtaa gctgtggggt gtcaccaagg acccctacgc caacggcttt 120

gctgctggtg gtgcctccaa gcccggccga acccgaaaca ttgttgaggc catggaggag 180

tccggcaaga actgtgttgt tttctacggc tcccagaccg gcaccgccga ggactacgcc 240

tctcgactgg ccaaggaagg caagtctcga ttcggtctga acaccatgat cgctgatctc 300

gaggactacg actttgacaa cctggacacc gtcccctccg acaacatcgt catgtttgtc 360

ctggccacct acggtgaggg tgagcccacc gacaacgccg tcgacttcta cgagttcatc 420

accggtgagg acgcttcttt caacgagggt aacgaccctc ctctcggcaa cctcaactac 480

gttgccttcg gtctgggcaa caacacctac gagcactaca actccatggt ccgaaacgtc 540

aacaaggctc tcgagaagct cggcgcccac cgaatcggtg aggctggtga gggtgacgac 600

ggtgccggta ccatggagga agatttcctg gcctggaagg accccatgtg ggaggctctc 660

gccaagaaga tgggcctcga ggagcgagag gccgtctacg agcccatctt tgccatcaac 720

gagcgagatg atctcacccc cgaggccaac gaggtctacc tcggtgagcc caacaagctc 780

cacctcgagg gtaccgccaa gggccccttc aactcccaca acccctacat tgcccccatt 840

gctgagtcct acgagctgtt ctccgccaag gaccgaaact gtctgcacat ggagattgac 900

atctccggct ccaacctcaa gtacgagact ggtgaccaca tcgccatctg gcccaccaac 960

cccggtgaag aggtcaacaa gttcctcgac atcctcgatc tctccggcaa gcagcactct 1020

gttgtcaccg tcaaggctct cgagcccacc gccaaggtcc ccttccccaa ccccaccacc 1080

tacgacgcca tcctgcgata ccacctcgag atctgtgccc ccgtttctcg acagttcgtt 1140

tccactctgg ctgcctttgc ccccaacgac gacatcaagg ccgagatgaa ccgactcggc 1200

tccgacaagg actacttcca cgagaaaacc ggtcctcact actacaacat tgctcgattc 1260

ctggcttctg tctccaaggg tgagaagtgg accaagatcc ccttctccgc cttcattgag 1320

ggtctgacca agctccagcc ccgatactac tccatctctt cttcttctct cgtccagccc 1380

aagaagatct ccatcaccgc tgttgtcgag tcccagcaga tccccggccg agatgacccc 1440

ttccgaggtg ttgccaccaa ctacctcttc gccctcaagc agaagcagaa cggtgacccc 1500

aaccccgctc ccttcggtca gtcttacgag ctcaccggcc cccgaaacaa gtacgacggt 1560

atccacgtcc ccgtccacgt ccgacactcc aacttcaagc tcccctccga ccccggcaag 1620

cccatcatca tgattggccc cggtactggt gttgctccct tccgaggctt tgtccaggag 1680

cgagccaagc aggctcgaga tggtgtcgag gtcggtaaga ctctgctctt cttcggctgc 1740

cgaaagtcca ccgaggactt catgtaccag aaggagtggc aggagtacaa agaggctctc 1800

ggtgacaagt tcgagatgat caccgccttc tcccgagagg gctccaagaa ggtctacgtc 1860

cagcaccgac tcaaggagcg atccaaggaa gtctccgacc tcctctccca gaaggcctac 1920

ttctacgtct gcggtgacgc cgcccacatg gcccgagagg tcaacaccgt tctggcccag 1980

atcattgccg agggccgagg tgtctccgag gccaagggcg aggagattgt caagaacatg 2040

cgatctgcca accagtacca ggtctgctcc gactttgtca ccctccactg caaggagact 2100

acttacgcca actccgagct ccaggaagat gtctggtct 2139

<210> 178

<211> 2076

<212> DNA

<213> Arabidopsis thaliana

<400> 178

atgacctctg ctctctacgc ctccgatctc ttcaagcagc tcaagtccat catgggtacc 60

gactccctgt ccgacgatgt cgttctggtc attgccacca cctctctggc tctggtcgcc 120

ggtttcgttg tcctcctctg gaagaaaacc accgctgacc gatccggcga gctcaagccc 180

ctcatgatcc ccaagtctct catggccaag gacgaggacg acgatctcga tctcggttcc 240

ggcaagaccc gagtctccat cttctttggc acccagaccg gtaccgccga gggtttcgcc 300

aaggctctct ccgaggagat caaggcccga tacgagaagg ccgccgtcaa ggtcatcgac 360

ctcgacgact acgctgctga cgacgaccag tacgaggaga agctcaagaa ggagactctc 420

gccttcttct gtgtcgccac ctacggtgac ggcgagccca ccgacaacgc cgcccgattc 480

tacaagtggt tcaccgagga gaacgagcga gacatcaagc tccagcagct ggcctacggt 540

gttttcgccc tcggcaaccg acagtacgag cacttcaaca agatcggtat tgtcctcgac 600

gaggagctct gcaagaaggg tgccaagcga ctcattgagg ttggtctggg tgatgatgac 660

cagtccattg aggacgactt caacgcctgg aaggagtctc tgtggtccga gctcgacaag 720

ctgctcaagg acgaggacga caagtccgtt gccaccccct acaccgccgt catccccgag 780

taccgagtcg tcacccacga cccccgattc accacccaga agtccatgga gtccaacgtt 840

gccaacggta acaccaccat tgacatccac cacccctgcc gagtcgacgt tgccgtccag 900

aaggagctcc acacccacga gtccgaccga tcttgtatcc acctcgagtt tgacatctcc 960

cgaaccggta tcacctacga gactggtgac cacgttggtg tctacgccga gaaccacgtt 1020

gagattgtcg aggaagctgg taagctgctc ggccactctc tcgacctcgt tttctccatc 1080

cacgccgaca aagaggacgg ctctcccctc gagtccgctg ttccccctcc cttccccggc 1140

ccctgcactc tcggcaccgg tctggcccga tacgctgacc tcctcaaccc tccccgaaag 1200

tccgctctgg ttgctctcgc cgcctacgcc accgagccct ccgaggccga gaagctcaag 1260

cacctgacct cccccgacgg caaggacgag tactcccagt ggatcgttgc ttcccagcga 1320

tctctgctcg aggtcatggc cgccttcccc tctgccaagc cccctctggg tgttttcttt 1380

gccgccattg ctccccgact ccagccccga tactactcca tctcctcctc tccccgactc 1440

gccccctctc gagtccacgt cacctctgct ctcgtctacg gtcccactcc cactggccga 1500

atccacaagg gtgtctgctc cacctggatg aagaacgccg tccccgctga gaagtcccac 1560

gagtgctccg gtgctcccat cttcatccga gcctccaact tcaagctccc ctccaacccc 1620

tccaccccca ttgtcatggt cggccccggt actggtctgg cccccttccg aggcttcctc 1680

caggagcgaa tggctctcaa ggaagatggt gaggagctcg gctcttctct gctcttcttt 1740

ggctgccgaa accgacagat ggacttcatc tacgaggacg agctcaacaa ctttgtcgac 1800

cagggtgtca tctccgagct catcatggcc ttctcccgag agggtgccca gaaggagtac 1860

gtccagcaca agatgatgga gaaggccgcc caggtctggg atctcatcaa ggaagagggc 1920

tacctctacg tctgtggtga cgccaagggc atggcccgag atgtccaccg aactctgcac 1980

accattgtcc aggagcagga aggtgtctct tcctccgagg ctgaggccat tgtcaagaag 2040

ctgcagaccg agggccgata cctgcgagat gtatgg 2076

<210> 179

<211> 2133

<212> DNA

<213> Arabidopsis thaliana

<400> 179

atgtcctcct cttcttcttc ttccacctcc atgattgatc tcatggctgc catcatcaag 60

ggtgagcccg tcattgtctc cgaccccgcc aacgcctccg cctacgagtc cgttgctgcc 120

gagctgtcct ccatgctcat cgagaaccga cagtttgcca tgatcgtcac cacctccatt 180

gctgttctca ttggctgcat tgtcatgctc gtctggcgac gatctggctc cggtaactcc 240

aagcgagtcg agcccctcaa gcccctggtc atcaagcccc gagaagagga gatcgacgac 300

ggccgaaaga aggtcaccat cttctttggc acccagaccg gtactgctga gggcttcgcc 360

aaggctctcg gtgaggaagc caaggctcga tacgaaaaga cccgattcaa gattgtcgac 420

ctcgatgatt acgctgccga tgacgacgag tacgaggaga agctcaagaa agaggacgtt 480

gccttcttct tcctcgccac ctacggtgac ggtgagccca ccgacaacgc tgcccgattc 540

tacaagtggt tcaccgaggg taacgaccga ggcgagtggc tcaagaacct caagtacggt 600

gttttcggtc tgggcaaccg acagtacgag cacttcaaca aggttgccaa ggttgtcgac 660

gacatcctcg tcgagcaggg tgcccagcga ctcgtccagg tcggcctcgg tgatgatgac 720

cagtgcatcg aggacgactt cactgcctgg cgagaggctc tgtggcccga gctcgacacc 780

attctgcgag aggaaggtga caccgccgtt gccaccccct acaccgccgc cgtcctcgag 840

taccgagtct ccatccacga ctccgaggat gccaagttca acgacatcaa catggccaac 900

ggtaacggct acaccgtctt tgacgcccag cacccctaca aggccaacgt cgccgtcaag 960

cgagagctcc acacccccga gtccgaccga tcttgtatcc acctcgagtt tgacattgct 1020

ggttccggtc tgacctacga gactggtgac cacgttggtg tcctctgtga caacctgtcc 1080

gagactgtcg acgaggctct gcgactcctc gacatgtccc ccgacactta cttctctctg 1140

cacgccgaga aagaggacgg tactcccatc tcttcttctc tgccccctcc cttccctccc 1200

tgcaacctgc gaaccgctct gacccgatac gcctgcctcc tctcttctcc caagaagtct 1260

gctctcgttg ctctggccgc ccacgcctcc gaccccaccg aggctgagcg actcaagcac 1320

ctcgcctctc ccgctggcaa ggacgagtac tccaagtggg ttgtcgagtc ccagcgatct 1380

ctgctcgagg tcatggccga gttcccctcc gccaagcccc ctctcggtgt tttcttcgcc 1440

ggtgttgctc cccgactcca gccccgattc tactccatct cctcttcccc caagatcgcc 1500

gagactcgaa tccacgttac ctgtgctctg gtctacgaga agatgcccac cggccgaatc 1560

cacaagggtg tctgctccac ctggatgaag aacgccgttc cctacgagaa gtccgagaac 1620

tgttcctctg ctcccatctt tgtccgacag tccaacttca agctcccctc cgactccaag 1680

gtccccatca tcatgattgg ccccggtacc ggcctcgccc ccttccgagg cttcctgcag 1740

gagcgactcg ccctcgtcga gtccggtgtc gagctcggcc cctccgtcct cttctttggc 1800

tgccgaaacc gacgaatgga cttcatctac gaagaggagc tccagcgatt cgtcgagtcc 1860

ggtgctctcg ccgagctctc cgttgccttc tcccgagagg gtcccaccaa ggagtacgtc 1920

cagcacaaga tgatggacaa ggcctccgac atctggaaca tgatctccca gggcgcctac 1980

ctctacgtct gcggtgacgc caagggtatg gcccgagatg tccaccgatc tctgcacacc 2040

attgcccagg agcagggctc catggactcc accaaggccg agggtttcgt caagaacctc 2100

cagacctccg gccgatacct ccgagatgtc tgg 2133

<210> 180

<211> 1575

<212> DNA

<213> Gibberella fujikuroi

<400> 180

atgtccaagt ccaactccat gaactccacc tcccacgaga ctctcttcca gcagctcgtt 60

ctcggcctcg accgaatgcc cctcatggac gtccactggc tcatctacgt tgcctttggt 120

gcctggctct gctcctacgt catccacgtt ctgtcctctt cctccactgt caaggtcccc 180

gtcgtcggtt accgatccgt tttcgagccc acctggctcc tccgactgcg attcgtctgg 240

gagggtggtt ccatcattgg ccagggctac aacaagttca aggactccat cttccaggtc 300

cgaaagctcg gtaccgacat tgtcatcatc cctcccaact acattgacga ggtccgaaag 360

ctctcccagg acaagacccg atccgtcgag cccttcatca acgactttgc cggccagtac 420

acccgaggta tggtctttct gcagtccgat ctccagaacc gagtcatcca gcagcgactc 480

acccccaagc ttgtctctct caccaaggtc atgaaggaag agctcgacta cgctctgacc 540

aaggagatgc ccgacatgaa gaacgacgag tgggttgagg tcgacatctc ttccatcatg 600

gtccgactca tctctcgaat ctccgcccga gttttcctcg gccccgagca ctgccgaaac 660

caggagtggc tcaccaccac cgccgagtac tccgagtctc tcttcatcac cggcttcatc 720

ctccgagttg tcccccacat tctccgaccc ttcattgctc ctctgctgcc ctcttaccga 780

accctgctgc gaaacgtttc ttccggccga cgagtcattg gtgatatcat ccgatcccag 840

cagggtgacg gtaacgagga catcctctct tggatgcgag atgctgccac tggtgaggag 900

aagcagatcg acaacattgc ccagcgaatg ctcattctgt ctctcgcctc catccacacc 960

accgccatga ccatgaccca cgccatgtac gatctgtgtg cctgccccga gtacattgag 1020

cccctccgag atgaggtcaa gtccgtcgtt ggtgcttctg gctgggacaa gaccgctctc 1080

aaccgattcc acaagctcga ctctttcctc aaggagtccc agcgattcaa ccccgttttc 1140

ctgctcacct tcaaccgaat ctaccaccag tccatgaccc tctccgatgg taccaacatc 1200

ccctccggta cccgaattgc tgtcccctct cacgccatgc tccaggactc cgcccacgtc 1260

cccggtccca ctcctcccac tgagttcgac ggtttccgat actccaagat ccgatccgac 1320

tccaactacg cccagaagta cctcttctcc atgaccgact cttccaacat ggcctttggc 1380

tacggtaagt acgcctgccc cggccgattc tacgcctcca acgagatgaa gctgactctg 1440

gccattctgc tcctccagtt tgagttcaag ctccccgacg gtaagggccg accccgaaac 1500

atcaccatcg actccgacat gatccccgac ccccgagctc gactctgtgt ccgaaagcga 1560

tctctgcgtg acgag 1575

<210> 181

<211> 1419

<212> DNA

<213> Stevia rebaudiana

<400> 181

atggccacct ccgactccat tgtcgacgac cgaaagcagc tgcacgttgc caccttcccc 60

tggctcgcct ttggccacat tctgccctac ctccagctct ccaagctcat tgctgagaag 120

ggccacaagg tttctttcct gtccaccacc cgaaacatcc agcgactctc ctcccacatc 180

tctcctctca tcaacgttgt ccagctcacc ctcccccgag tccaggagct ccccgaggat 240

gccgaggcca ccactgatgt ccaccccgag gacatcccct acctcaagaa ggcctccgac 300

ggtctgcagc ccgaggtcac ccgattcctc gagcagcact ctcccgactg gatcatctac 360

gactacaccc actactggct cccctccatt gctgcttctc tcggtatctc tcgagcccac 420

ttctccgtca ccaccccctg ggccattgct tacatgggcc cctctgctga cgccatgatc 480

aacggttccg acggccgaac caccgtcgag gatctcacca cccctcccaa gtggttcccc 540

ttccccacca aggtctgctg gcgaaagcac gatctcgccc gactcgtccc ctacaaggcc 600

cccggtatct ccgacggtta ccgaatgggt ctggttctca agggctccga ctgtctgctc 660

tccaagtgct accacgagtt tggtacccag tggctccccc tgctcgagac tctgcaccag 720

gtccccgttg tccccgtcgg tctgctccct cccgagatcc ccggtgacga gaaggacgag 780

acttgggttt ccatcaagaa gtggctcgac ggcaagcaga agggctccgt cgtctacgtt 840

gctctcggct ccgaggttct tgtctcccag actgaggtcg tcgagctcgc cctcggtctg 900

gagctctccg gtctgccctt cgtctgggcc taccgaaagc ccaagggtcc cgccaagtcc 960

gactccgtcg agctccccga cggtttcgtc gagcgaactc gagatcgagg tctggtctgg 1020

acctcttggg ctccccagct ccgaatcctc tcccacgagt ccgtctgcgg tttcctgacc 1080

cactgtggtt ccggctccat tgtcgagggc ctcatgttcg gccaccccct catcatgctg 1140

cccatcttcg gtgaccagcc cctcaacgcc cgactcctcg aggacaagca ggtcggtatc 1200

gagatccccc gaaacgaaga ggacggctgc ctcaccaagg agtctgttgc ccgatctctg 1260

cgatctgttg ttgtcgagaa agagggtgag atctacaagg ccaacgcccg agagctctcc 1320

aagatctaca acgacaccaa ggtcgagaag gagtacgttt cccagtttgt cgactacctc 1380

gagaagaacg cccgagctgt cgccattgac cacgagagt 1419

<210> 182

<211> 2552

<212> DNA

<213> 人工序列

<220>

<223> tCPS-cwpT

<400> 182

cccactagtt ataaagtcac aagtatctca gtatacccgt ctaaccacac atttatcacc 60

atgtgcaagg ctgtttccaa ggagtactcc gatctgctcc agaaggacga ggcctctttc 120

accaagtggg acgacgacaa ggtcaaggac cacctcgaca ccaacaagaa cctctacccc 180

aacgacgaga tcaaggagtt tgtcgagtcc gtcaaggcca tgttcggctc catgaacgac 240

ggcgagatta atgtctctgc ttacgacacc gcctgggttg ctctggtcca ggatgtcgac 300

ggttccggct ctcctcagtt cccttcctct ctcgagtgga tcgccaacaa ccagctgtcc 360

gacggttctt ggggtgacca cctgctcttc tctgctcacg accgaatcat caacaccctg 420

gcctgtgtca ttgctctgac ctcttggaac gtccacccct ccaagtgcga gaagggtctg 480

aacttcctcc gagagaacat ctgcaagctc gaggacgaga acgccgagca catgcccatt 540

ggcttcgagg tcaccttccc ctctctgatt gacattgcca agaagctcaa cattgaggtc 600

cccgaggaca cccccgctct caaggagatc tacgctcgac gagacatcaa gctcaccaag 660

atccccatgg aggttctcca caaggtcccc accactctcc tccactctct cgagggtatg 720

cccgatctcg agtgggagaa gctgctcaag ctgcagtgca aggacggctc tttcctcttc 780

tccccctctt ccactgcctt cgccctcatg cagaccaagg acgagaagtg tctccagtac 840

ctcaccaaca ttgtcaccaa gttcaacggt ggtgtcccca acgtctaccc cgttgacctc 900

tttgagcaca tctgggttgt tgaccgactc cagcgactcg gtatcgcccg atacttcaag 960

tccgagatca aggactgtgt cgagtacatc aacaagtact ggaccaagaa cggtatctgc 1020

tgggcccgaa acacccacgt ccaggacatt gacgacaccg ccatgggctt ccgagttctg 1080

cgagcccacg gctacgatgt cacccccgat gtctttcgac agtttgagaa ggacggcaag 1140

tttgtctgtt tcgccggtca gtccacccag gccgtcaccg gtatgttcaa cgtctaccga 1200

gcttctcaga tgctcttccc cggtgagcga atcctcgagg acgccaagaa gttctcctac 1260

aactacctca aggagaagca gtccaccaac gagctgctcg acaagtggat cattgccaag 1320

gatctgcccg gtgaggttgg ctacgccctc gacatcccct ggtacgcctc tctgccccga 1380

ctggagactc gatactacct cgagcagtac ggtggtgagg acgatgtctg gatcggtaag 1440

accctgtacc gaatgggcta cgtttccaac aacacctacc tcgagatggc caagctcgac 1500

tacaacaact acgttgccgt cctccagctc gagtggtaca ccatccagca gtggtacgtc 1560

gacattggta tcgagaagtt cgagtccgac aacatcaagt ccgtccttgt ctcctactac 1620

ctcgctgctg cctccatctt cgagcccgag cgatccaagg agcgaattgc ctgggccaag 1680

accaccatcc tcgtcgacaa gatcacctcc atcttcgact cctcccagtc ctccaaggaa 1740

gatatcaccg ccttcattga caagttccga aacaagtcct cctccaagaa gcactccatc 1800

aacggcgagc cctggcacga ggtcatggtt gctctcaaga aaactctcca cggctttgcc 1860

ctcgacgctc tgatgaccca ctctcaggac atccaccccc agctccacca ggcctgggag 1920

atgtggctca ccaagctcca ggacggtgtt gatgtcactg ctgagctcat ggtccagatg 1980

atcaacatga ccgccggccg atgggtttcc aaggagctcc tcacccaccc ccagtaccag 2040

cgactctcca ctgtcaccaa ctctgtctgc cacgacatca ccaagctcca caacttcaag 2100

gagaactcca ccaccgtcga ctccaaggtc caggagctgg tccagctcgt tttctccgac 2160

acccccgatg atctcgacca ggacatgaag cagaccttcc tgactgtcat gaaaactttc 2220

tactacaagg cctggtgcga ccccaacacc atcaacgacc acatctccaa ggtctttgag 2280

attgtgattt aagttttttg atcaatgatc caatggcttt cacatacccc cccacgccta 2340

taattaaaac acagagaaat ataatctaac ttaataaata ttacggagaa tctttcgagt 2400

gttcagcaga aatatagcca ttgtaacaaa agccggctat cgaccgcttt atcgaagaat 2460

atttcccgcc ccccagtggc caaacgatat cgaaacaaaa gagctgaaat catatccttc 2520

agtagtagta tagtcctgtt atcacagcat ca 2552

<210> 183

<211> 2394

<212> DNA

<213> 人工序列

<220>

<223> tKS

<400> 183

agatatacaa gcaatgtcac tctccttcgt actcgtacat acaacacaac tacattcaaa 60

atgacctccc acggcggcca gaccaacccc accaacctca tcattgacac caccaaggag 120

cgaatccaga agcagttcaa gaacgtcgag atctccgttt cctcctacga caccgcctgg 180

gtcgccatgg tcccctctcc caactccccc aagtctccct gcttccccga gtgtctcaac 240

tggctcatca acaaccagct caacgacggc tcttggggtc tggtcaacca cacccacaac 300

cacaaccacc ccctcctcaa ggactctctc tcttccactc tcgcctgcat tgttgctctc 360

aagcgatgga acgttggcga ggaccagatc aacaagggtc tgtctttcat tgagtccaac 420

ctcgcctccg ccaccgagaa gtcccagccc tcccccattg gctttgatat catcttcccc 480

ggtctgctcg agtacgccaa gaacctcgat atcaacctgc tctccaagca gaccgacttc 540

tctctcatgc tgcacaagcg agagctcgag cagaagcgat gccactccaa cgagatggac 600

ggctacctgg cctacatttc cgagggtctg ggtaacctct acgactggaa catggtcaag 660

aagtaccaga tgaagaacgg ttccgttttc aactccccct ctgccaccgc tgctgccttc 720

atcaaccacc agaaccccgg ctgtctcaac tacctcaact ctctgctcga caagtttggt 780

aacgccgtcc ccactgtcta cccccacgat ctcttcatcc gactctccat ggtcgacacc 840

attgagcgac tcggtatttc ccaccacttc cgagtcgaga tcaagaacgt tctcgatgag 900

acttaccgat gctgggttga gcgagatgag cagatcttca tggacgttgt cacctgtgct 960

ctggccttcc gactcctccg aatcaacggt tacgaggttt cccccgaccc cctcgccgag 1020

atcaccaacg agctggctct caaggacgag tacgccgccc tcgagactta ccacgcttct 1080

cacattctgt accaagagga tctgtcctcc ggcaagcaga ttctcaagtc cgccgacttc 1140

ctcaaggaga tcatctccac tgactccaac cgactctcca agctcatcca caaggaagtc 1200

gagaacgctc tcaagttccc catcaacacc ggtctggagc gaatcaacac ccgacgaaac 1260

atccagctct acaacgtcga caacacccga attctcaaga ccacctacca ctcttccaac 1320

atctccaaca ccgactacct gcgactcgcc gtcgaggact tctacacctg ccagtccatc 1380

taccgagagg agctcaaggg tctggagcga tgggttgtcg agaacaagct cgaccagctc 1440

aagtttgccc gacaaaagac tgcctactgc tacttctccg ttgctgccac cctctcttct 1500

cccgagctct ccgacgcccg aatctcttgg gccaagaacg gtatcctgac cactgttgtc 1560

gacgacttct ttgacattgg tggcaccatt gacgagctga ccaacctcat ccagtgcgtc 1620

gagaagtgga acgtcgacgt tgacaaggac tgttgttccg agcacgtccg aatcctcttc 1680

ctggctctca aggacgccat ctgctggatc ggtgacgagg ccttcaagtg gcaggctcga 1740

gatgtcactt cccacgtcat ccagacctgg ctcgagctca tgaactccat gctgcgagag 1800

gccatctgga cccgagatgc ctacgtcccc accctcaacg agtacatgga gaacgcctac 1860

gtcagctttg ctctcggtcc cattgtcaag cccgccatct actttgtcgg tcccaagctg 1920

tccgaggaga ttgtcgagtc ctccgagtac cacaacctct tcaagctcat gtccacccag 1980

ggccgactcc tcaacgatat ccactccttc aagcgagagt tcaaggaagg taagctcaac 2040

gccgttgctc tgcacctgtc caacggtgag tccggcaagg tcgaggaaga ggtcgtcgag 2100

gagatgatga tgatgatcaa gaacaagcga aaggagctca tgaagctcat cttcgaggag 2160

aacggctcca ttgtcccccg agcctgcaag gacgccttct ggaacatgtg ccacgtcctc 2220

aacttcttct acgccaacga cgacggtttc accggcaaca ccattctcga caccgtcaag 2280

gacatcatct acaaccctct ggttctggtc aacgagaacg aggagcagag gtaactatcc 2340

gaagatcaag agcgaagcaa gttgtaagtc caggacatgt ttcccgccca cgcg 2394

<210> 184

<211> 2979

<212> DNA

<213> 人工序列

<220>

<223> CPSKS

<400> 184

cccactagtt ataaagtcac aagtatctca gtatacccgt ctaaccacac atttatcacc 60

atgcccggca agattgagaa cggtaccccc aaggacctca agaccggcaa cgacttcgtt 120

tccgctgcca agtctctgct cgaccgagcc ttcaagtccc accactccta ctacggtctg 180

tgctccactt cctgccaggt ctacgacacc gcctgggttg ccatgatccc caagacccga 240

gacaacgtca agcagtggct cttccccgag tgtttccact acctgctcaa gacccaggct 300

gctgacggct cttggggctc tctgcccacc acccagaccg ccggtatcct cgacactgcc 360

tccgccgttc tggctctgct ctgccacgcc caggagcctc tgcagatcct cgatgtctcc 420

cccgatgaga tgggtctgcg aatcgagcac ggtgtcactt ctctcaagcg acagctcgcc 480

gtctggaacg acgttgagga caccaaccac attggtgtcg agttcatcat ccccgctctg 540

ctctccatgc tcgagaagga gctcgatgtc ccctccttcg agttcccttg ccgatccatt 600

ctggagcgaa tgcacggcga gaagctcggt cactttgatc tcgagcaggt ctacggcaag 660

ccctcttctc tgctgcactc tctcgaggcc ttcctcggca agctcgattt cgaccgactc 720

tcccaccacc tctaccacgg ctccatgatg gcctccccct cttccaccgc cgcctacctc 780

attggtgcca ccaagtggga cgacgaggcc gaggactacc tccgacacgt catgcgaaac 840

ggtgccggcc acggtaacgg tggtatctct ggtaccttcc ccaccaccca ctttgagtgc 900

tcctggatca ttgccactct cctcaaggtc ggtttcactc tcaagcagat tgacggtgac 960

ggtctgcgag gcctctccac cattctcctc gaggctctgc gtgacgagaa cggtgtcatt 1020

ggcttcgctc cccgaaccgc cgacgttgat gacactgcca aggctctgct ggctctgtct 1080

ctggtcaacc agcccgtttc ccccgacatc atgatcaagg ttttcgaggg taaggaccac 1140

ttcaccacct ttggctccga gcgagatccc tctctgacct ccaacctcca cgttctcctc 1200

tctctgctca agcagtccaa cctgtctcag taccaccccc agatcctcaa gaccactctc 1260

ttcacctgtc gatggtggtg gggctccgac cactgtgtca aggacaagtg gaacctctcc 1320

cacctctacc ccaccatgct cctcgtcgag gccttcaccg aggttctgca cctcatcgac 1380

ggtggtgagc tctcctctct gtttgacgag tctttcaagt gcaagatcgg tctgtccatc 1440

ttccaggccg ttctccgaat catcctcacc caggacaacg acggctcttg gcgaggctac 1500

cgagagcaga cctgttacgc cattctcgcc ctcgtccagg cccgacacgt ctgtttcttc 1560

acccacatgg tcgaccgact ccagtcctgt gtcgaccgag gtttctcttg gctcaagtct 1620

tgctccttcc actctcagga cctcacctgg acctccaaga ccgcttacga ggttggtttc 1680

gttgccgagg cctacaagct cgctgctctc cagtccgcct ctctcgaggt ccccgctgcc 1740

accatcggcc actccgtcac ctctgctgtc ccctcctccg atctcgagaa gtacatgcga 1800

ctcgtccgaa agaccgctct gttctctccc ctcgatgagt ggggtctgat ggcctccatc 1860

atcgagtcct ccttctttgt ccccctcctc caggcccagc gagttgagat ctacccccga 1920

gacaacatca aggtcgacga ggacaagtac ctctccatca tccccttcac ctgggttggc 1980

tgcaacaacc gatctcgaac ctttgcctcc aaccgatggc tctacgacat gatgtacctg 2040

tctctgctcg gctaccagac cgacgagtac atggaggccg ttgccggccc cgtctttggt 2100

gatgtctctc tgctgcacca gaccatcgac aaggtcattg acaacaccat gggtaacctg 2160

gcccgagcca acggtaccgt ccactccggc aacggtcacc agcacgagtc ccccaacatt 2220

ggccaggtcg aggacaccct cactcgattt accaactccg tcctcaacca caaggacgtc 2280

ctcaactcct cctcctccga ccaggacacc ctccgacgag agttccgaac cttcatgcac 2340

gcccacatca cccagatcga ggacaactcc cgattctcca agcaggcttc ttccgacgcc 2400

ttctcctccc ccgagcagtc ctacttccag tgggtcaact ccactggtgg ttcccacgtt 2460

gcctgcgcct actctttcgc cttctccaac tgcctcatgt ccgccaacct cctccagggt 2520

aaggacgcct tcccttccgg tacccagaag tacctcatct cctccgtcat gcgacacgcc 2580

accaacatgt gccgaatgta caacgatttc ggttccatcg cccgagacaa cgccgagcga 2640

aacgtcaact ccatccactt tcccgagttc actctctgta acggtacctc ccagaacctc 2700

gacgagcgaa aggagcgact cctcaagatt gctacttacg agcagggcta cctcgaccga 2760

gctctcgagg ctctggagcg acagtcccga gatgatgctg gtgaccgagc cggctccaag 2820

gacatgcgaa agctcaagat tgtcaagctc ttctgtgacg tcaccgacct ctacgaccag 2880

ctctacgtca tcaaggacct ctcctcctcc atgaaataac tatccgaaga tcaagagcga 2940

agcaagttgt aagtccagga catgtttccc gcccacgcg 2979

<210> 185

<211> 1698

<212> DNA

<213> 人工序列

<220>

<223> KAH4

<400> 185

ttgaatcttt tttcttcttc tcttctctat attcattctt gaattaaaca cacatcaaca 60

atggagtctc tggttgtcca caccgtcaac gccatctggt gcattgtcat tgtcggtatc 120

ttctccgtcg gctaccacgt ctacggccga gctgttgtcg agcagtggcg aatgcgacga 180

tctctcaagc tccagggtgt caagggtcct cctccctcca tcttcaacgg taacgtttcc 240

gagatgcagc gaatccagtc cgaggccaag cactgctccg gtgacaacat catctcccac 300

gactactctt cttctctgtt cccccacttt gaccactggc gaaagcagta cggccgaatc 360

tacacctact ccactggcct caagcagcac ctctacatca accaccccga gatggtcaag 420

gagctctccc agaccaacac cctcaacctc ggccgaatca cccacatcac caagcgactc 480

aaccccattc tcggtaacgg tatcatcacc tccaacggcc cccactgggc ccaccagcga 540

cgaatcattg cctacgagtt cacccacgac aagatcaagg gtatggtcgg tctgatggtc 600

gagtccgcca tgcccatgct caacaagtgg gaggagatgg tcaagcgagg tggtgagatg 660

ggctgtgaca tccgagtcga cgaggacctc aaggatgtct ccgctgacgt cattgccaag 720

gcctgtttcg gctcttcctt ctccaagggc aaggccatct tctccatgat ccgagatctg 780

ctcaccgcca tcaccaagcg atccgtcctc ttccgattca acggtttcac cgacatggtt 840

ttcggctcca agaagcacgg tgacgttgac attgacgctc tcgagatgga gctcgagtcc 900

tccatctggg agactgtcaa ggagcgagag attgagtgca aggacaccca caagaaggac 960

ctcatgcagc tcattctcga gggtgccatg cgatcttgtg acggtaacct gtgggacaag 1020

tctgcttacc gacgattcgt tgtcgacaac tgcaagtcca tctactttgc cggccacgac 1080

tccaccgccg tttccgtttc ttggtgcctc atgctgctcg ctctcaaccc ctcttggcag 1140

gtcaagatcc gagatgagat tctgtcctcc tgcaagaacg gtatccccga cgccgagtcc 1200

atccccaacc tcaagaccgt caccatggtc atccaggaga ctatgcgact ctaccctccc 1260

gctcccattg tcggccgaga ggcctccaag gacattcgac tcggtgatct ggttgtcccc 1320

aagggtgtct gtatctggac cctcatcccc gctctgcacc gagatcccga gatctggggt 1380

cccgacgcca acgacttcaa gcccgagcga ttctccgagg gtatctccaa ggcctgcaag 1440

tacccccagt cctacatccc ctttggcctc ggcccccgaa cctgtgtcgg caagaacttt 1500

ggtatgatgg aggtcaaggt cctcgtttct ctgattgtct ccaagttctc cttcactctg 1560

tctcccacct accagcactc tccctcccac aagctgctcg tcgagcccca gcacggtgtt 1620

gtcatccgag ttgtataaac ttcgagctaa tccagtagct tacgttaccc aggggcaggt 1680

caactggcta gccacgag 1698

<210> 186

<211> 1698

<212> DNA

<213> 人工序列

<220>

<223> KOgib

<400> 186

cacacccgaa atcgttaagc atttccttct gagtataaga atcattcgct agccacaaaa 60

atgtccaagt ccaactccat gaactccacc tcccacgaga ctctcttcca gcagctcgtt 120

ctcggcctcg accgaatgcc cctcatggac gtccactggc tcatctacgt tgcctttggt 180

gcctggctct gctcctacgt catccacgtt ctgtcctctt cctccactgt caaggtcccc 240

gtcgtcggtt accgatccgt tttcgagccc acctggctcc tccgactgcg attcgtctgg 300

gagggtggtt ccatcattgg ccagggctac aacaagttca aggactccat cttccaggtc 360

cgaaagctcg gtaccgacat tgtcatcatc cctcccaact acattgacga ggtccgaaag 420

ctctcccagg acaagacccg atccgtcgag cccttcatca acgactttgc cggccagtac 480

acccgaggta tggtctttct gcagtccgat ctccagaacc gagtcatcca gcagcgactc 540

acccccaagc ttgtctctct caccaaggtc atgaaggaag agctcgacta cgctctgacc 600

aaggagatgc ccgacatgaa gaacgacgag tgggttgagg tcgacatctc ttccatcatg 660

gtccgactca tctctcgaat ctccgcccga gttttcctcg gccccgagca ctgccgaaac 720

caggagtggc tcaccaccac cgccgagtac tccgagtctc tcttcatcac cggcttcatc 780

ctccgagttg tcccccacat tctccgaccc ttcattgctc ctctgctgcc ctcttaccga 840

accctgctgc gaaacgtttc ttccggccga cgagtcattg gtgatatcat ccgatcccag 900

cagggtgacg gtaacgagga catcctctct tggatgcgag atgctgccac tggtgaggag 960

aagcagatcg acaacattgc ccagcgaatg ctcattctgt ctctcgcctc catccacacc 1020

accgccatga ccatgaccca cgccatgtac gatctgtgtg cctgccccga gtacattgag 1080

cccctccgag atgaggtcaa gtccgtcgtt ggtgcttctg gctgggacaa gaccgctctc 1140

aaccgattcc acaagctcga ctctttcctc aaggagtccc agcgattcaa ccccgttttc 1200

ctgctcacct tcaaccgaat ctaccaccag tccatgaccc tctccgatgg taccaacatc 1260

ccctccggta cccgaattgc tgtcccctct cacgccatgc tccaggactc cgcccacgtc 1320

cccggtccca ctcctcccac tgagttcgac ggtttccgat actccaagat ccgatccgac 1380

tccaactacg cccagaagta cctcttctcc atgaccgact cttccaacat ggcctttggc 1440

tacggtaagt acgcctgccc cggccgattc tacgcctcca acgagatgaa gctgactctg 1500

gccattctgc tcctccagtt tgagttcaag ctccccgacg gtaagggccg accccgaaac 1560

atcaccatcg actccgacat gatccccgac ccccgagctc gactctgtgt ccgaaagcga 1620

tctctgcgtg acgagtaagc tatttacagc atgtgtaatg aggaatataa cgttgattga 1680

attgtttgtg aaaaatgt 1698

<210> 187

<211> 2262

<212> DNA

<213> 人工序列

<220>

<223> CPR1

<400> 187

cgtccatata tatctatgct gcgtcgtcct tttcgtgaca tcaccaaaac acatacaaaa 60

atggctgagc tcgacactct cgacattgtt gttctcggtg tcatcttcct cggtaccgtt 120

gcctacttca ccaagggtaa gctgtggggt gtcaccaagg acccctacgc caacggcttt 180

gctgctggtg gtgcctccaa gcccggccga acccgaaaca ttgttgaggc catggaggag 240

tccggcaaga actgtgttgt tttctacggc tcccagaccg gcaccgccga ggactacgcc 300

tctcgactgg ccaaggaagg caagtctcga ttcggtctga acaccatgat cgctgatctc 360

gaggactacg actttgacaa cctggacacc gtcccctccg acaacatcgt catgtttgtc 420

ctggccacct acggtgaggg tgagcccacc gacaacgccg tcgacttcta cgagttcatc 480

accggtgagg acgcttcttt caacgagggt aacgaccctc ctctcggcaa cctcaactac 540

gttgccttcg gtctgggcaa caacacctac gagcactaca actccatggt ccgaaacgtc 600

aacaaggctc tcgagaagct cggcgcccac cgaatcggtg aggctggtga gggtgacgac 660

ggtgccggta ccatggagga agatttcctg gcctggaagg accccatgtg ggaggctctc 720

gccaagaaga tgggcctcga ggagcgagag gccgtctacg agcccatctt tgccatcaac 780

gagcgagatg atctcacccc cgaggccaac gaggtctacc tcggtgagcc caacaagctc 840

cacctcgagg gtaccgccaa gggccccttc aactcccaca acccctacat tgcccccatt 900

gctgagtcct acgagctgtt ctccgccaag gaccgaaact gtctgcacat ggagattgac 960

atctccggct ccaacctcaa gtacgagact ggtgaccaca tcgccatctg gcccaccaac 1020

cccggtgaag aggtcaacaa gttcctcgac atcctcgatc tctccggcaa gcagcactct 1080

gttgtcaccg tcaaggctct cgagcccacc gccaaggtcc ccttccccaa ccccaccacc 1140

tacgacgcca tcctgcgata ccacctcgag atctgtgccc ccgtttctcg acagttcgtt 1200

tccactctgg ctgcctttgc ccccaacgac gacatcaagg ccgagatgaa ccgactcggc 1260

tccgacaagg actacttcca cgagaaaacc ggtcctcact actacaacat tgctcgattc 1320

ctggcttctg tctccaaggg tgagaagtgg accaagatcc ccttctccgc cttcattgag 1380

ggtctgacca agctccagcc ccgatactac tccatctctt cttcttctct cgtccagccc 1440

aagaagatct ccatcaccgc tgttgtcgag tcccagcaga tccccggccg agatgacccc 1500

ttccgaggtg ttgccaccaa ctacctcttc gccctcaagc agaagcagaa cggtgacccc 1560

aaccccgctc ccttcggtca gtcttacgag ctcaccggcc cccgaaacaa gtacgacggt 1620

atccacgtcc ccgtccacgt ccgacactcc aacttcaagc tcccctccga ccccggcaag 1680

cccatcatca tgattggccc cggtactggt gttgctccct tccgaggctt tgtccaggag 1740

cgagccaagc aggctcgaga tggtgtcgag gtcggtaaga ctctgctctt cttcggctgc 1800

cgaaagtcca ccgaggactt catgtaccag aaggagtggc aggagtacaa agaggctctc 1860

ggtgacaagt tcgagatgat caccgccttc tcccgagagg gctccaagaa ggtctacgtc 1920

cagcaccgac tcaaggagcg atccaaggaa gtctccgacc tcctctccca gaaggcctac 1980

ttctacgtct gcggtgacgc cgcccacatg gcccgagagg tcaacaccgt tctggcccag 2040

atcattgccg agggccgagg tgtctccgag gccaagggcg aggagattgt caagaacatg 2100

cgatctgcca accagtacca ggtctgctcc gactttgtca ccctccactg caaggagact 2160

acttacgcca actccgagct ccaggaagat gtctggtctt aaaattaaca gatagtttgc 2220

cggtgataat tctcttaacc tcccacactc ctttgacata ac 2262

<210> 188

<211> 2256

<212> DNA

<213> 人工序列

<220>

<223> CPR3

<400> 188

cgtccatata tatctatgct gcgtcgtcct tttcgtgaca tcaccaaaac acatacaaaa 60

atgtcctcct cttcttcttc ttccacctcc atgattgatc tcatggctgc catcatcaag 120

ggtgagcccg tcattgtctc cgaccccgcc aacgcctccg cctacgagtc cgttgctgcc 180

gagctgtcct ccatgctcat cgagaaccga cagtttgcca tgatcgtcac cacctccatt 240

gctgttctca ttggctgcat tgtcatgctc gtctggcgac gatctggctc cggtaactcc 300

aagcgagtcg agcccctcaa gcccctggtc atcaagcccc gagaagagga gatcgacgac 360

ggccgaaaga aggtcaccat cttctttggc acccagaccg gtactgctga gggcttcgcc 420

aaggctctcg gtgaggaagc caaggctcga tacgaaaaga cccgattcaa gattgtcgac 480

ctcgatgatt acgctgccga tgacgacgag tacgaggaga agctcaagaa agaggacgtt 540

gccttcttct tcctcgccac ctacggtgac ggtgagccca ccgacaacgc tgcccgattc 600

tacaagtggt tcaccgaggg taacgaccga ggcgagtggc tcaagaacct caagtacggt 660

gttttcggtc tgggcaaccg acagtacgag cacttcaaca aggttgccaa ggttgtcgac 720

gacatcctcg tcgagcaggg tgcccagcga ctcgtccagg tcggcctcgg tgatgatgac 780

cagtgcatcg aggacgactt cactgcctgg cgagaggctc tgtggcccga gctcgacacc 840

attctgcgag aggaaggtga caccgccgtt gccaccccct acaccgccgc cgtcctcgag 900

taccgagtct ccatccacga ctccgaggat gccaagttca acgacatcaa catggccaac 960

ggtaacggct acaccgtctt tgacgcccag cacccctaca aggccaacgt cgccgtcaag 1020

cgagagctcc acacccccga gtccgaccga tcttgtatcc acctcgagtt tgacattgct 1080

ggttccggtc tgacctacga gactggtgac cacgttggtg tcctctgtga caacctgtcc 1140

gagactgtcg acgaggctct gcgactcctc gacatgtccc ccgacactta cttctctctg 1200

cacgccgaga aagaggacgg tactcccatc tcttcttctc tgccccctcc cttccctccc 1260

tgcaacctgc gaaccgctct gacccgatac gcctgcctcc tctcttctcc caagaagtct 1320

gctctcgttg ctctggccgc ccacgcctcc gaccccaccg aggctgagcg actcaagcac 1380

ctcgcctctc ccgctggcaa ggacgagtac tccaagtggg ttgtcgagtc ccagcgatct 1440

ctgctcgagg tcatggccga gttcccctcc gccaagcccc ctctcggtgt tttcttcgcc 1500

ggtgttgctc cccgactcca gccccgattc tactccatct cctcttcccc caagatcgcc 1560

gagactcgaa tccacgttac ctgtgctctg gtctacgaga agatgcccac cggccgaatc 1620

cacaagggtg tctgctccac ctggatgaag aacgccgttc cctacgagaa gtccgagaac 1680

tgttcctctg ctcccatctt tgtccgacag tccaacttca agctcccctc cgactccaag 1740

gtccccatca tcatgattgg ccccggtacc ggcctcgccc ccttccgagg cttcctgcag 1800

gagcgactcg ccctcgtcga gtccggtgtc gagctcggcc cctccgtcct cttctttggc 1860

tgccgaaacc gacgaatgga cttcatctac gaagaggagc tccagcgatt cgtcgagtcc 1920

ggtgctctcg ccgagctctc cgttgccttc tcccgagagg gtcccaccaa ggagtacgtc 1980

cagcacaaga tgatggacaa ggcctccgac atctggaaca tgatctccca gggcgcctac 2040

ctctacgtct gcggtgacgc caagggtatg gcccgagatg tccaccgatc tctgcacacc 2100

attgcccagg agcagggctc catggactcc accaaggccg agggtttcgt caagaacctc 2160

cagacctccg gccgatacct ccgagatgtc tggtaaaatt aacagatagt ttgccggtga 2220

taattctctt aacctcccac actcctttga cataac 2256

<210> 189

<211> 1566

<212> DNA

<213> 人工序列

<220>

<223> UGT1

<400> 189

cccactagtt ataaagtcac aagtatctca gtatacccgt ctaaccacac atttatcacc 60

atggacgcca tggccaccac cgagaagaag ccccacgtca tcttcatccc cttccccgcc 120

cagtcccaca tcaaggccat gctcaagctc gcccagctcc tccaccacaa gggcctccag 180

atcacctttg tcaacaccga cttcatccac aaccagttcc tcgagtcctc cggcccccac 240

tgtctggacg gtgctcccgg tttccgattt gagactatcc ccgatggtgt ctcccactcc 300

cccgaggcct ccatccccat ccgagagtct ctgctccgat ccattgagac taacttcctc 360

gaccgattca ttgatctcgt caccaagctc cccgatcctc ccacctgtat catctccgac 420

ggtttcctgt ccgttttcac cattgatgct gccaagaagc tcggtatccc cgtcatgatg 480

tactggactc tggctgcctg tggtttcatg ggtttctacc acatccactc tctgatcgag 540

aagggctttg ctcctctcaa ggacgcctcc tacctcacca acggttacct cgacaccgtc 600

attgactggg tccccggtat ggagggtatc cgactcaagg acttccccct cgactggtcc 660

accgacctca acgacaaggt tctcatgttc accaccgagg ctccccagcg atcccacaag 720

gtttcccacc acatcttcca caccttcgac gagctcgagc cctccatcat caagactctg 780

tctctgcgat acaaccacat ctacaccatt ggccccctcc agctcctcct cgaccagatc 840

cccgaggaga agaagcagac cggtatcacc tctctgcacg gctactctct cgtcaaggaa 900

gagcccgagt gcttccagtg gctccagtcc aaggagccca actccgttgt ctacgtcaac 960

tttggctcca ccaccgtcat gtctctcgag gacatgaccg agtttggctg gggtctggcc 1020

aactccaacc actacttcct gtggatcatc cgatccaacc tcgtcattgg cgagaacgcc 1080

gttctgcctc ccgagctcga ggagcacatc aagaagcgag gcttcattgc ctcttggtgc 1140

tcccaggaga aggttctcaa gcacccctcc gtcggtggtt tcctgaccca ctgcggctgg 1200

ggctccacca ttgagtctct gtccgctggt gtccccatga tctgctggcc ctactcctgg 1260

gaccagctca ccaactgccg atacatctgc aaggagtggg aggttggtct ggagatgggt 1320

accaaggtca agcgagatga ggtcaagcga ctcgtccagg agctcatggg cgagggtggt 1380

cacaagatgc gaaacaaggc caaggactgg aaggagaagg cccgaattgc cattgccccc 1440

aacggctctt cttctctcaa cattgacaag atggtcaagg agatcactgt tctcgctcga 1500

aactaactat ccgaagatca agagcgaagc aagttgtaag tccaggacat gtttcccgcc 1560

cacgcg 1566

<210> 190

<211> 1503

<212> DNA

<213> 人工序列

<220>

<223> UGT3

<400> 190

ttgaatcttt tttcttcttc tcttctctat attcattctt gaattaaaca cacatcaaca 60

atggccgagc agcagaagat caagaagtct ccccacgttc tgctcatccc cttccctctg 120

cagggccaca tcaacccctt catccagttc ggcaagcgac tcatctccaa gggtgtcaag 180

accactctgg tcaccaccat ccacaccctc aactccactc tcaaccactc caacaccacc 240

accacctcca tcgagatcca ggccatctcc gacggctgtg acgagggtgg tttcatgtct 300

gctggtgagt cttacctcga gactttcaag caggtcggtt ccaagtctct ggctgacctc 360

atcaagaagc tccagtccga gggtaccacc attgacgcca tcatctacga ctccatgacc 420

gagtgggttc tcgatgtcgc catcgagttt ggtattgacg gtggctcctt cttcacccag 480

gcctgtgtcg tcaactctct ctactaccac gtccacaagg gtctgatctc tctgcccctc 540

ggcgagactg tctccgtccc cggtttcccc gttctgcagc gatgggagac tcctctcatt 600

ctccagaacc acgagcagat ccagtccccc tggtcccaga tgctcttcgg ccagttcgcc 660

aacattgacc aggcccgatg ggttttcacc aactccttct acaagctcga ggaagaggtc 720

attgagtgga cccgaaagat ctggaacctc aaggtcattg gccccaccct cccctccatg 780

tacctcgaca agcgactcga tgacgacaag gacaacggtt tcaacctcta caaggccaac 840

caccacgagt gcatgaactg gctcgacgac aagcccaagg agtccgttgt ctacgttgcc 900

tttggctctc tggtcaagca cggccccgag caggttgagg agatcacccg agctctgatt 960

gactccgatg tcaacttcct gtgggtcatc aagcacaagg aagagggtaa gctccccgag 1020

aacctgtccg aggtcatcaa gaccggcaag ggcctcattg ttgcctggtg caagcagctc 1080

gacgttctcg cccacgagtc cgtcggctgc tttgtcaccc actgcggttt caactccacc 1140

ctcgaggcta tctctctcgg tgtccccgtt gttgccatgc cccagttctc cgaccagacc 1200

accaacgcca agctcctcga tgagattctc ggtgtcggtg tccgagtcaa ggctgacgag 1260

aacggtattg tccgacgagg taacctggct tcttgtatca agatgatcat ggaggaagag 1320

cgaggtgtca tcatccgaaa gaacgccgtc aagtggaagg atctggccaa ggttgctgtc 1380

cacgagggtg gctcttccga caacgacatt gtcgagtttg tctccgagct catcaaggcc 1440

taaacttcga gctaatccag tagcttacgt tacccagggg caggtcaact ggctagccac 1500

gag 1503

<210> 191

<211> 1497

<212> DNA

<213> 人工序列

<220>

<223> UGT4

<400> 191

cacacccgaa atcgttaagc atttccttct gagtataaga atcattcgct agccacaaaa 60

atggagaaca agaccgagac taccgtccga cgacgacgac gaatcattct cttccccgtc 120

cccttccagg gccacatcaa ccccattctg cagctcgcca acgttctgta ctccaagggc 180

ttctccatca ccatcttcca caccaacttc aacaagccca agacctccaa ctacccccac 240

ttcactttcc gattcatcct cgacaacgac ccccaggacg agcgaatctc caacctgccc 300

acccacggtc ctctggctgg tatgcgaatc cccatcatca acgagcacgg tgctgacgag 360

ctccgacgag agctcgagct gctcatgctc gcctccgaag aggacgagga agtctcctgt 420

ctgatcaccg atgctctgtg gtactttgcc cagtccgtcg ccgactctct caacctgcga 480

cgactcgttc tcatgacctc ctctctgttc aacttccacg cccacgtttc tctgccccag 540

tttgacgagc tcggttacct cgaccccgat gacaagaccc gactcgagga gcaggcttcc 600

ggtttcccca tgctcaaggt caaggacatc aagtccgcct actccaactg gcagattctc 660

aaggagattc tcggcaagat gatcaagcag accaaggcct cctccggtgt catctggaac 720

tccttcaagg agctcgagga gtccgagctc gagactgtca tccgagagat ccccgctccc 780

tctttcctca tccccctgcc caagcacctc accgcttcct cctcttctct gctcgaccac 840

gaccgaaccg tctttcagtg gctcgaccag cagccccctt cctccgtcct ctacgtttcc 900

ttcggctcca cctccgaggt cgacgagaag gacttcctcg agattgctcg aggcctcgtt 960

gactccaagc agtccttcct gtgggttgtc cgacccggct ttgtcaaggg ctccacctgg 1020

gttgagcccc tgcccgatgg tttcctcggt gagcgaggcc gaattgtcaa gtgggtcccc 1080

cagcaggaag ttctggccca cggtgccatt ggtgccttct ggacccactc cggctggaac 1140

tccactctcg agtccgtctg cgagggtgtc cccatgatct tctccgactt tggcctcgac 1200

cagcccctca acgcccgata catgtccgat gttctcaagg tcggtgtcta cctcgagaac 1260

ggctgggagc gaggtgagat tgccaacgcc atccgacgag tcatggtcga cgaggaaggt 1320

gagtacatcc gacagaacgc ccgagtcctc aagcagaagg ccgatgtctc tctcatgaag 1380

ggtggttctt cttacgagtc tctcgagtct ctcgtttcct acatctcttc tttgtaagct 1440

atttacagca tgtgtaatga ggaatataac gttgattgaa ttgtttgtga aaaatgt 1497

<210> 192

<211> 1542

<212> DNA

<213> 人工序列

<220>

<223> UGT9

<400> 192

cgtccatata tatctatgct gcgtcgtcct tttcgtgaca tcaccaaaac acatacaaaa 60

atggccacct ccgactccat tgtcgacgac cgaaagcagc tgcacgttgc caccttcccc 120

tggctcgcct ttggccacat tctgccctac ctccagctct ccaagctcat tgctgagaag 180

ggccacaagg tttctttcct gtccaccacc cgaaacatcc agcgactctc ctcccacatc 240

tctcctctca tcaacgttgt ccagctcacc ctcccccgag tccaggagct ccccgaggat 300

gccgaggcca ccactgatgt ccaccccgag gacatcccct acctcaagaa ggcctccgac 360

ggtctgcagc ccgaggtcac ccgattcctc gagcagcact ctcccgactg gatcatctac 420

gactacaccc actactggct cccctccatt gctgcttctc tcggtatctc tcgagcccac 480

ttctccgtca ccaccccctg ggccattgct tacatgggcc cctctgctga cgccatgatc 540

aacggttccg acggccgaac caccgtcgag gatctcacca cccctcccaa gtggttcccc 600

ttccccacca aggtctgctg gcgaaagcac gatctcgccc gactcgtccc ctacaaggcc 660

cccggtatct ccgacggtta ccgaatgggt ctggttctca agggctccga ctgtctgctc 720

tccaagtgct accacgagtt tggtacccag tggctccccc tgctcgagac tctgcaccag 780

gtccccgttg tccccgtcgg tctgctccct cccgagatcc ccggtgacga gaaggacgag 840

acttgggttt ccatcaagaa gtggctcgac ggcaagcaga agggctccgt cgtctacgtt 900

gctctcggct ccgaggttct tgtctcccag actgaggtcg tcgagctcgc cctcggtctg 960

gagctctccg gtctgccctt cgtctgggcc taccgaaagc ccaagggtcc cgccaagtcc 1020

gactccgtcg agctccccga cggtttcgtc gagcgaactc gagatcgagg tctggtctgg 1080

acctcttggg ctccccagct ccgaatcctc tcccacgagt ccgtctgcgg tttcctgacc 1140

cactgtggtt ccggctccat tgtcgagggc ctcatgttcg gccaccccct catcatgctg 1200

cccatcttcg gtgaccagcc cctcaacgcc cgactcctcg aggacaagca ggtcggtatc 1260

gagatccccc gaaacgaaga ggacggctgc ctcaccaagg agtctgttgc ccgatctctg 1320

cgatctgttg ttgtcgagaa agagggtgag atctacaagg ccaacgcccg agagctctcc 1380

aagatctaca acgacaccaa ggtcgagaag gagtacgttt cccagtttgt cgactacctc 1440

gagaagaacg cccgagctgt cgccattgac cacgagagtt aaaattaaca gatagtttgc 1500

cggtgataat tctcttaacc tcccacactc ctttgacata ac 1542

<210> 193

<211> 880

<212> DNA

<213> 人工序列

<220>

<223> pTPI

<400> 193

aaacaaaaga gctgaaatca tatccttcag tagtagtata gtcctgttat cacagcatca 60

attacccccg tccaagtaag ttgattggga tttttgttta cagatacagt aatatacttg 120

actatttctt tacaggtgac tcagaaagtg catgttggaa atgagccaca gaccaagaca 180

agatatgaca aaattgcact attcgatgca gaattcgacg gtgtttccat tggtgttatg 240

acattcatct gcattcatac aaaaaagtct tggtagtggt acttttgcgt tattacctcc 300

gatatctacg caccccccaa cccccctgct acagtaaaga gtgtgagtct actgtacatg 360

cttactaaac cacctactgt acagcgaaac ccctcagcaa aatcacacaa tcagctcatt 420

acaacacacc caatgacctc accacaaatt ctatacgcct tttgacgcca ttattacagt 480

agcttgcaac gccgttgtct taggttccat ttttagtgct ctattacctc acttaacccg 540

tataggcaga tcaggccatg gcactaagtg tagagctaga ggttgatatc gccacgagtg 600

ctccatcagg gctagggtgg ggttagaaat acagtccgtg cgcactcaaa aggcgtccgg 660

gttagggcat ccgataatat cgcctggact cggcgccata ttctcgactt ctgggcgcgt 720

tgtattcatc tcctccgctt cccaacactt ccacccgttt ctccatccca accaatagaa 780

tagggtaacc ttattcggga cactttcgtc atacatagtc agatatacaa gcaatgtcac 840

tctccttcgt actcgtacat acaacacaac tacattcaaa 880

<210> 194

<211> 1183

<212> DNA

<213> 人工序列

<220>

<223> gpdT-pGPD

<400> 194

ctatccgaag atcaagagcg aagcaagttg taagtccagg acatgtttcc cgcccacgcg 60

agtgatttat aacacctctc ttttttgaca cccgctcgcc ttgaaattca tgtcacataa 120

attatagtca acgacgtttg aataacttgt cttgtagttc gatgatgatc atatgattac 180

attaatagta attactgtat ggcgcgccgg tagtcggaaa gagccgggac cggccggcga 240

gcataaaccg gacgcagtag gatgtcctgc acgggtcttt ttgtggggtg tggagaaagg 300

ggtgcttgga gatggaagcc ggtagaaccg ggctgcttgg ggggatttgg ggccgctggg 360

ctccaaagag gggtaggcat ttcgttgggg ttacgtaatt gcggcatttg ggtcctgcgc 420

gcatgtccca ttggtcagaa ttagtccgga taggagactt atcagccaat cacagcgccg 480

gatccacctg taggttgggt tgggtgggag cacccctcca cagagtagag tcaaacagca 540

gcagcaacat gatagttggg ggtgtgcgtg ttaaaggaaa aaaaaagaag cttgggttat 600

attcccgctc tatttagagg ttgcgggata gacgccgacg gagggcaatg gcgccatgga 660

accttgcgga tatcgatacg ccgcggcgga ctgcgtccga accagctcca gcagcgtttt 720

ttccgggcca ttgagccgac tgcgaccccg ccaacgtgtc ttggcccacg cactcatgtc 780

atgttggtgt tgggaggcca ctttttaagt agcacaaggc acctagctcg cagcaaggtg 840

tccgaaccaa agaagcggct gcagtggtgc aaacggggcg gaaacggcgg gaaaaagcca 900

cgggggcacg aattgaggca cgccctcgaa tttgagacga gtcacggccc cattcgcccg 960

cgcaatggct cgccaacgcc cggtcttttg caccacatca ggttacccca agccaaacct 1020

ttgtgttaaa aagcttaaca tattataccg aacgtaggtt tgggcgggct tgctccgtct 1080

gtccaaggca acatttatat aagggtctgc atcgccggct caattgaatc ttttttcttc 1140

ttctcttctc tatattcatt cttgaattaa acacacatca aca 1183

<210> 195

<211> 639

<212> DNA

<213> 人工序列

<220>

<223> pgmT-pTEF

<400> 195

acttcgagct aatccagtag cttacgttac ccaggggcag gtcaactggc tagccacgag 60

tctgtcccag gtcgcaattt agtgtaataa acaatatata tattgagtct aaagggaatt 120

gtagctattg tgattgtgtg attttcgtct tgctggttct tattgtgtcc cattcgtttc 180

atcctgatga ggacccctgg cgtacgccgg cgaagcttgg taccagagac gggttggcgg 240

cgtatttgtg tcccaaaaaa cagccccaat tgccccaatt gaccccaaat tgacccagta 300

gcgggcccaa ccccggcgag agcccccttc accccacata tcaaacctcc cccggttccc 360

acacttgccg ttaagggcgt agggtactgc agtctggaat ctacgcttgt tcagactttg 420

tactagtttc tttgtctggc catccgggta acccatgccg gacgcaaaat agactactga 480

aaattttttt gctttgtggt tgggacttta gccaagggta taaaagacca ccgtccccga 540

attacctttc ctcttctttt ctctctctcc ttgtcaactc acacccgaaa tcgttaagca 600

tttccttctg agtataagaa tcattcgcta gccacaaaa 639

<210> 196

<211> 1009

<212> DNA

<213> 人工序列

<220>

<223> pgkT-pPGM

<400> 196

gctatttaca gcatgtgtaa tgaggaatat aacgttgatt gaattgtttg tgaaaaatgt 60

agaaaatttc agtgaagttg tgttttctat atagtaagca cttttggtac aagtatctgc 120

acatccctgc atgttacaag cctgatcatg cagggcaata ttctgactat aaatatacct 180

cgatatttta gcaagctata cgtacgtacc aaccacagat tacgacccat tcgcagtcac 240

agttcactag ggtttgggtt gcatccgttg agagtggttt gtttttaacc ttctccatgt 300

gctcactcag gttttgggtt cagatcaaat caaggcgtga accactgttt gaggacaaat 360

gtgacacaac caaccagtgt caggggcaag tccgtgacaa aggggaagat acaatgcaat 420

tactgacagt tacggactgc ctcgatgccc taaccttgcc ccaaaataag acaactgtcc 480

tcgtttaagc gcaaccctat tcagcgtcac gtcataatag cgtttggata gcactagtct 540

atgaggagcg ttttatgttg cggtgagggc gattggtgct catatgggtt caattgaggt 600

ggtggaacga gcttagtctt caattgaggt gcgagcgaca caattgggtg tcacgtggcc 660

taattgacct cggatcgtgg agtccccagt tatacagcaa ccacgaggtg catgagtagg 720

agacgtcacc agacaatagg gtttttttgg actggagagg gtagggcaaa agcgctcaac 780

gggctgtttg gggagctatg ggggaggaat tggcgatatt tgtgaggttg acggctccga 840

tttgcgtgtt ttgtcgcttc tgcatctccc catacccata tcttccctcc ccacctcttt 900

ccacgataat tttacggatc agcaataagg ttccttctcc tagtttccac gtccatatat 960

atctatgctg cgtcgtcctt ttcgtgacat caccaaaaca catacaaaa 1009

<210> 197

<211> 2472

<212> DNA

<213> 人工序列

<220>

<223> LEU2 and flanking sequences

<400> 197

aaaattaaca gatagtttgc cggtgataat tctcttaacc tcccacactc ctttgacata 60

acgatttatg taacgaaact gaaatttgac cagatattgt tgtaaataga aaatctggct 120

tgtaggtgga aactagtaac ggccgccagt gtgctggaat tcggctccac tgtcctccac 180

tacaaacaca cccaatctgc ttcttctagt caaggttgct acaccggtaa attataaatc 240

atcatttcat tagcagggct gggccctttt tatagagtct tatacactag cggaccctgc 300

cggtagacca acccgcaggc gcgtcagttt gctccttcca tcaatgcgtc gtagaaacga 360

cttactcctt cttgagcagc tccttgacct tgttggcaac aaagtctccg acctcggagg 420

tggaggaaga gcctccgata tcggcggtag tgataccagc ctcgacggac tccttgacgg 480

cagcctcaac agcgtcaccg gcgggcttca tgttaagaga gaacttgagc atcatggcgg 540

cagacagaat ggtggcaatg gggttgacct tctgcttgcc gagatcgggg gcagatccgt 600

gacagggctc gtacagaccg aacgcctcgt tggtgtcggg cagagaagcc agagaggcgg 660

agggcagcag acccagagaa ccggggatga cggaggcctc gtcggagatg atatcgccaa 720

acatgttggt ggtgatgatg ataccattca tcttggaggg ctgcttgatg aggatcatgg 780

cggccgagtc gatcagctgg tggttgagct cgagctgggg gaattcgtcc ttgaggactc 840

gagtgacagt ctttcgccaa agtcgagagg aggccagcac gttggccttg tcaagagacc 900

acacgggaag aggggggttg tgctgaaggg ccaggaaggc ggccattcgg gcaattcgct 960

caacctcagg aacggagtag gtctcggtgt cggaagcgac gccagatccg tcatcctcct 1020

ttcgctctcc aaagtagata cctccgacga gctctcggac aatgatgaag tcggtgccct 1080

caacgtttcg gatgggggag agatcggcga gcttgggcga cagcagctgg cagggtcgca 1140

ggttggcgta caggttcagg tcctttcgca gcttgaggag accctgctcg ggtcgcacgt 1200

cggttcgtcc gtcgggagtg gtccatacgg tgttggcagc gcctccgaca gcaccgagca 1260

taatagagtc agcctttcgg cagatgtcga gagtagcgtc ggtgatgggc tcgccctcct 1320

tctcaatggc agctcctcca atgagtcggt cctcaaacac aaactcggtg ccggaggcct 1380

cagcaacaga cttgagcacc ttgacggcct cggcaatcac ctcggggcca cagaagtcgc 1440

cgccgagaag aacaatcttc ttggagtcag tcttggtctt cttagtttcg ggttccattg 1500

tggatgtgtg tggttgtatg tgtgatgtgg tgtgtggagt gaaaatctgt ggctggcaaa 1560

cgctcttgta tatatacgca cttttgcccg tgctatgtgg aagactaaac ctccgaagat 1620

tgtgactcag gtagtgcggt atcggctagg gacccaaacc ttgtcgatgc cgatagcgct 1680

atcgaacgta ccccagccgg ccgggagtat gtcggagggg acatacgaga tcgtcaaggg 1740

tttgtggcca actggtaaat aaatgatgac tcaggcgacg acggaattcg acagcaacta 1800

ctcctttcac caaccatgtg cattttagct cgaataacat tcacaggctt ggtgatctac 1860

atccatggtg tctggccgat taccgtggtg ttttggcagt aacgagaata ttgagtggac 1920

tcttcccatc accaataaag actcatacta caatcacgag cgcttcagct gccactatag 1980

tgttggtgac acaatacccc tcgatgctgg gcattactgt agcaagagat attatttcat 2040

ggcgcatttt tccagtctac ctgacttttt agtgtgattt cttctccaca ttttatgctc 2100

agtgtgaaaa gttggagtgc acacttaatt atcgccggtt ttcggaaagt actatgtgct 2160

caaggttgca ccccacgtta cgtatgcagc acattgagca gcctttggac cgtggagata 2220

acggtgtgga gatagcaacg ggtagtcttc gtaataagca atgcagccga attctgcaga 2280

tatccatcac actggcggcc gctcgagcat gcatctagat ggcctccttg gccgggtttc 2340

aattcaattc atcatttttt ttttattctt ttttttgatt tcggtttctt tgaaattttt 2400

ttgattcggt aatctccgaa cagaaggaag aacgaaggaa ggagcacaga cttagattgg 2460

tatatatacg ca 2472

<210> 198

<211> 6989

<212> DNA

<213> 人工序列

<220>

<223> 载体序列

<400> 198

ctagatggcc tccttggccg ggtttcaatt caattcatca tttttttttt attctttttt 60

ttgatttcgg tttctttgaa atttttttga ttcggtaatc tccgaacaga aggaagaacg 120

aaggaaggag cacagactta gattggtata tatacgcata tgtagtgttg aagaaacatg 180

aaattgccca gtattcttaa cccaactgca cagaacaaaa acctgcagga aacgaagata 240

aatcatgtcg aaagctacat ataaggaacg tgctgctact catcctagtc ctgttgctgc 300

caagctattt aatatcatgc acgaaaagca aacaaacttg tgtgcttcat tggatgttcg 360

taccaccaag gaattactgg agttagttga agcattaggt cccaaaattt gtttactaaa 420

aacacatgtg gatatcttga ctgatttttc catggagggc acagttaagc cgctaaaggc 480

attatccgcc aagtacaatt ttttactctt cgaagacaga aaatttgctg acattggtaa 540

tacagtcaaa ttgcagtact ctgcgggtgt atacagaata gcagaatggg cagacattac 600

gaatgcacac ggtgtggtgg gcccaggtat tgttagcggt ttgaagcagg cggcagaaga 660

agtaacaaag gaacctagag gccttttgat gttagcagaa ttgtcatgca agggctccct 720

atctactgga gaatatacta agggtactgt tgacattgcg aagagcgaca aagattttgt 780

tatcggcttt attgctcaaa gagacatggg tggaagagat gaaggttacg attggttgat 840

tatgacaccc ggtgtgggtt tagatgacaa gggagacgca ttgggtcaac agtatagaac 900

cgtggatgat gtggtctcta caggatctga cattattatt gttggaagag gactatttgc 960

aaagggaagg gatgctaagg tagagggtga acgttacaga aaagcaggct gggaagcata 1020

tttgagaaga tgcggccagc aaaactaaaa aactgtatta taagtaaatg catgtatact 1080

aaactcacaa attagagctt caatttaatt atatcagtta ttacccggga atctcggtcg 1140

taatgatttt tataatgacg aaaaaaaaaa aattggaaag aaaacccccc ccccgcagcg 1200

ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc cggctaggct 1260

ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga acgtgaagcg 1320

actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc ggtttccgtg 1380

tttcgtaaag tctggaaacg cggaagtcag cgccctgcac cattatgttc cggatctgca 1440

tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg aagcgctggc 1500

attgaccctg agtgattttt ctctggtccc gccgcatcca taccgccagt tgtttaccct 1560

cacaacgttc cagtaaccgg gcatgttcat catcagtaac ccgtatcgtg agcatcctct 1620

ctcgtttcat cggtatcatt acccccatga acagaaattc ccccttacac ggaggcatca 1680

agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt 1740

aacgcttctg gagaaactca acgagctgga cgcggatgaa caggcagaca tctgtgaatc 1800

gcttcacgac cacgctgatg agctttaccg caggtgggcc attctcatga agaatatctt 1860

gaatttattg tcatattact agttggtgtg gaagtccata tatcggtgat caatatagtg 1920

gttgacatgc tggctagtca acattgagcc ttttgatcat gcaaatatat tacggtattt 1980

tacaatcaaa tatcaaactt aactattgac tttataactt atttaggtgg taacattctt 2040

ataaaaaaga aaaaaattac tgcaaaacag tactagcttt taacttgtat cctaggttat 2100

ctatgctgtc tcaccataga gaatattacc tatttcagaa tgtatgtcca tgattcgccg 2160

ggtaaataca tataatacac aaatctggct taataaagtc tataatatat ctcataaaga 2220

agtgctaaat tggctagtgc tatatatttt taagaaaatt tcttttgact aagtccatat 2280

cgactttgta aaagttcact ttagcataca tatattacac gagccagaaa ttgtaacttt 2340

tgcctaaaat cacaaattgc aaaatttaat tgcttgcaaa aggtcacatg cttataatca 2400

acttttttaa aaatttaaaa tactttttta ttttttattt ttaaacataa atgaaataat 2460

ttatttattg tttatgatta ccgaaacata aaacctgctc aagaaaaaga aactgttttg 2520

tccttggaaa aaaagcacta cctaggagcg gccaaaatgc cgaggctttc atagcttaaa 2580

ctctttacag aaaataggca ttatagatca gttcgagttt tcttattctt ccttccggtt 2640

ttatcgtcac agttttacag taaataagta tcacctctta gagttaacta tgagataagc 2700

aagtatcatc tcatttcatt tacctgaagt cgagtaaaca gaaaatccaa ttgttgatga 2760

acctcaatga cttagaacta tctatcggca gatcatataa agaggattta ggtacctaga 2820

ggactgtacc tggagtatat atatatatat atatatatta tctcaactat agtccataga 2880

ggtttctttc ttgaggcctt aaactgctaa agaatgatat tggtggaatg caagcaccaa 2940

tctctcttct ttcgtaactg ttcatatact tcaaaccaag aatgtaacgg gcattgaccc 3000

atccaaaacc ttcagtagct gcccctttaa agtcagcacc ttgattaccg tattctgctt 3060

caacacgatg aggatctgtt cctcttgtga catcatattt ttcaaccaca ataccattat 3120

aatcgacaaa agcctttgtc atcatgaaaa gccatctata agctagccta ttcgttacag 3180

ttaaataacc ataagaacgg aggccttccc aagcaagaat ttgatggggt gcccaaccaa 3240

atggatagtc ccattgtcta attggtctcg aaatagaaat tgggcctcga gaacgctccg 3300

tacatgcagc taaacctcca agcatctcta acttgggtag tgctttctcc accattttct 3360

gtgcttgctc cttcgtggca agtccagccc ataatgccca gaatgtagtt gcggattcgt 3420

atgacgttct gtgcttgatt tttgtgttgt agtcaaagaa aaaccccgac tcgtcatccc 3480

acatatattt ggtaattgat gaggcaacgc taattatcaa catatagatt gttatctatc 3540

tgcatgaaca cgaaatcttt acttgacgac ttgaggctga tggtgtttat gcaaagaaac 3600

cactgtgttt aatatgtgtc actgtttgat attactgtca gcgtagaaga taatagtaaa 3660

agcggttaat aagtgtattt gagataagtg tgataaagtt tttacagcga aaagacgata 3720

aatacaagaa aatgattacg aggatacgga gagaggtatg tacatgtgta tttatatact 3780

aagctgccgg cggttgtttg caagaccgag aaaaggctag caagaatcgg gtcattgtag 3840

cgtatgcgcc tgtgaacatt ctcttcaaca agtttgattc cattgcggtg aaatggtaaa 3900

agtcaacccc ctgcgatgta tattttcctg tacaatcaat caaaaagcca aatgatttag 3960

cattatcttt acatcttgtt attttacaga ttttatgttt agatctttta tgcttgcttt 4020

tcaaaaggcc tgcaggcaag tgcacaaaca atacttaaat aaatactact cagtaataac 4080

ctatttctta gcatttttga cgaaatttgc tattttgtta gagtctttta caccatttgt 4140

ctccacacct ccgcttacat caacaccaat aacgccattt aatctaagcg catcaccaac 4200

attttctggc gtcagtccac cagctaacat aaaatgtaag ctctgcctcg cgcgtttcgg 4260

tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta 4320

agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg 4380

gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg 4440

gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc 4500

gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc 4560

tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 4620

acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 4680

aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 4740

cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 4800

gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 4860

tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 4920

tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 4980

cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 5040

gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 5100

ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt 5160

ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 5220

ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 5280

agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 5340

aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 5400

atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 5460

tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 5520

tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 5580

tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 5640

gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 5700

tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 5760

ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg 5820

gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 5880

aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 5940

ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 6000

tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 6060

ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta 6120

aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 6180

ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 6240

ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 6300

agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 6360

tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 6420

ataggggttt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 6480

tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttggccacct 6540

aggccggcct tcccggaagt atatactaat tcattccttg aatatttatg aaaagcaaca 6600

tgtgtatttc ttgtgtgtgc ggcaaacgta gcaattgcaa ctgcataaac gatgattgta 6660

aaagtatcac actttgctca gacaggttag attcacctgg tacgagggca gtgtcttaaa 6720

ggttccatct acctcggccc ttgtttcttg aagagtggtc aatatgtgtt ttatacagct 6780

gaaatttccc ctgtatgttg agatcgtgta tattggtcat aatctgggct ctttagtcga 6840

tcccagtttt ctcgggcaag tttttttctc cacaaagtac cgctggaaaa ctctatgtga 6900

cttgttgaca gattacttgg gttatctgcg ggatatgtct tggataggca accgggcata 6960

tatcaccggg cggactgttg gttctgtac 6989

<210> 199

<211> 1170

<212> DNA

<213> 人工序列

<220>

<223> pENO

<400> 199

tcgggcaagt ttttttctcc acaaagtacc gctggaaaac tctatgtgac ttgttgacag 60

attacttggg ttatctgcgg gatatgtctt ggataggcaa ccgggcatat atcaccgggc 120

ggactgttgg ttctgtacgt acatacagca ctttgagctc atgtctcaca cgcaaccatg 180

gtgcgtggag gctttggcat cctttctact tgtagtggct atagtacttg cagtccaagc 240

aaacatgagt atgtgcttgt atgtactgaa acccgtctac ggtaatattt tagagtgtgg 300

aactatggga tgagtgctca ttcgatacta tgttgtcacc cgatttgccg tttgcgaggt 360

aagacacatt cggtggttca ggcggctact tgtatgtagc atccacgttc atgttttgtg 420

gatcagatta atggtatgga tatgcacggg gcgtttcccc ggtaacgtgt aggcagtcca 480

gtgcaaccca gacagctgag ctctctatag ccgtgcgtgt gcggtcatat cacgctacac 540

ttagctacag aataaagctc ggtagcgcca acagcgttga caaatagctc aagggcgtgg 600

agcacagggt ttaggaggtt ttaatgggcg agaaggcgcg tagatgtagt cttcctcggt 660

cccatcggta atcacgtgtg tgccgatttg caagacgaaa agccacgaga ataaaccggg 720

agaggggatg gaagtccccg aacagcaacc agcccttgcc ctcgtggaca taacctttca 780

cttgccagaa ctctaagcgt caccacggta tacaagcgca cgtagaagat tgtggaagtc 840

gtgttggaga ctgttgattt gggcggtgga ggggggtatt tgagagcaag tttgagattt 900

gtgccattga gggggaggtt attgtggcca tgcagtcgga tttgccgtca cgggaccgca 960

acatgctttt cattgcagtc cttcaactat ccatctcacc tcccccaatg gcttttaact 1020

ttcgaatgac gaaagcaccc ccctttgtac agatgactat ttgggaccaa tccaatagcg 1080

caattgggtt tgcatcatgt ataaaaggag caatccccca ctagttataa agtcacaagt 1140

atctcagtat acccgtctaa ccacacattt 1170

<210> 200

<211> 2276

<212> DNA

<213> 人工序列

<220>

<223> HPH

<400> 200

aaattaacag atagtttgcc ggtgataatt ctcttaacct cccacactcc tttgacataa 60

cgatttatgt aacgaaactg aaatttgacc agatattgtt gtaaatagaa aatctggctt 120

gtaggtggca aactagtaac ggccgccagt gtgctggaat tgaatattta ccgttcgtat 180

aatgtatgct atacgaagtt ataccggtct cgtagtgttc acgttcagtt cacggtgagc 240

ttaaaactat cttcaagaag agatttgaga cctgatttat acttgcagca atgtttactt 300

cttatcgcga tacacgaatg tgatacggat caaagtaagc aggactacga taagataacg 360

aatgcggtgc agtccatgtc gattaggtat agatacattt attttgtgtt atgttacatt 420

ttggggggat actgtcctac ttgtagtacc tacttgtagt ggcgcgtcta ttcctttgcc 480

ctcggacgag tgctggggcg tcggtttcca ctatcggcga gtacttctac acagccatcg 540

gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat 600

cggacgattg cgtcgcatcg accctgcgcc caagctgcat catcgaaatt gccgtcaacc 660

aagctctgat agagttggtc aagaccaatg cggagcatat acgcccggag ccgcggcgat 720

cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct gctgctccat acaagccaac 780

cacggcctcc agaagaagat gttggcgacc tcgtattggg aatccccgaa catcgcctcg 840

ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa 900

tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat cagctcatcg 960

agagcctgcg cgacggacgc actgacggtg tcgtccatca cagtttgcca gtgatacaca 1020

tggggatcag caatcgcgca tatgaaatca cgccatgtag tgtattgacc gattccttgc 1080

ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat cgcatccatg 1140

gcctccgcga ccggctgcag aacagcgggc agttcggttt caggcaggtc ttgcaacgtg 1200

acaccctgtg cacggcggga gatgcaatag gtcaggctct cgctgaattc cccaatgtca 1260

agcacttccg gaatcgggag cgcggccgat gcaaagtgcc gataaacata acgatctttg 1320

tagaaaccat cggcgcagct atttacccgc aggacatatc cacgccctcc tacatcgaag 1380

ctgaaagcac gagattcttc gccctccgag agctgcatca ggtcggagac gctgtcgaac 1440

ttttcgatca gaaacttctc gacagacgtc gcggtgagtt caggcttttt catatgggta 1500

cctgagaaca tttttgtgtc taggtgtttg tgtttggact gcgatcagtg aagaaaagaa 1560

gaggaaaaat tgtgcaagaa attttgcttt caagacttgg ctgatgcagc agggtaactc 1620

tgggacacag acctatgttt gtggttaaac tcaatgcacg tggtacgtgc gtggagcgct 1680

tacccatcca agggtgtgga catggaaccg acggtccgtg gagttgtgta atgtcatttt 1740

ggcgactctt gaagcaaggc tataaaaaaa ttgtgtggct tgagtcttat cgagctcggt 1800

cactacaaga gttaatcttc ctgtctcagg cagacaggtc aggcagggtt acttttgggt 1860

gtgctgtaac tcactgtatg gccgttagtg cgcatagacg ttgtacatac tggaccgaat 1920

tgtagcgtgc tcaatagggc caataaagct attgtaggga tccataactt cgtataatgt 1980

atgctatacg aacggtaccc gggcaattct gcagatatcc atcacactgg cggccgctcg 2040

agcatgcatc tagatggcct ccttggccgg gtttcaattc aattcatcat ttttttttta 2100

ttcttttttt tgatttcggt ttctttgaaa tttttttgat tcggtaatct ccgaacagaa 2160

ggaagaacga aggaaggagc acagacttag attggtatat atacgcatat gtagtgttga 2220

agaaacatga aattgcccag tattcttaac ccaactgcac agaacaaaaa cctgca 2276

<210> 201

<211> 600

<212> DNA

<213> 人工序列

<220>

<223> Sc Eno2.启动子

<400> 201

gtgtcgacgc tgcgggtata gaaagggttc tttactctat agtacctcct cgctcagcat 60

ctgcttcttc ccaaagatga acgcggcgtt atgtcactaa cgacgtgcac caacttgcgg 120

aaagtggaat cccgttccaa aactggcatc cactaattga tacatctaca caccgcacgc 180

cttttttctg aagcccactt tcgtggactt tgccatatgc aaaattcatg aagtgtgata 240

ccaagtcagc atacacctca ctagggtagt ttctttggtt gtattgatca tttggttcat 300

cgtggttcat taattttttt tctccattgc tttctggctt tgatcttact atcatttgga 360

tttttgtcga aggttgtaga attgtatgtg acaagtggca ccaagcatat ataaaaaaaa 420

aaagcattat cttcctacca gagttgattg ttaaaaacgt atttatagca aacgcaattg 480

taattaattc ttattttgta tcttttcttc ccttgtctca atcttttatt tttattttat 540

ttttcttttc ttagtttctt tcataacacc aagcaactaa tactataaca tacaataata 600

<210> 202

<211> 600

<212> DNA

<213> 人工序列

<220>

<223> Sc Fba1.启动子

<400> 202

ctacttggct tcacatacgt tgcatacgtc gatatagata ataatgataa tgacagcagg 60

attatcgtaa tacgtaatag ttgaaaatct caaaaatgtg tgggtcatta cgtaaataat 120

gataggaatg ggattcttct atttttcctt tttccattct agcagccgtc gggaaaacgt 180

ggcatcctct ctttcgggct caattggagt cacgctgccg tgagcatcct ctctttccat 240

atctaacaac tgagcacgta accaatggaa aagcatgagc ttagcgttgc tccaaaaaag 300

tattggatgg ttaataccat ttgtctgttc tcttctgact ttgactcctc aaaaaaaaaa 360

aatctacaat caacagatcg cttcaattac gccctcacaa aaactttttt ccttcttctt 420

cgcccacgtt aaattttatc cctcatgttg tctaacggat ttctgcactt gatttattat 480

aaaaagacaa agacataata cttctctatc aatttcagtt attgttcttc cttgcgttat 540

tcttctgttc ttctttttct tttgtcatat ataaccataa ccaagtaata catattcaaa 600

<210> 203

<211> 600

<212> DNA

<213> 人工序列

<220>

<223> Sc Tef1.启动子

<400> 203

ttggctgata atagcgtata aacaatgcat actttgtacg ttcaaaatac aatgcagtag 60

atatatttat gcatattaca tataatacat atcacatagg aagcaacagg cgcgttggac 120

ttttaatttt cgaggaccgc gaatccttac atcacaccca atcccccaca agtgatcccc 180

cacacaccat agcttcaaaa tgtttctact ccttttttac tcttccagat tttctcggac 240

tccgcgcatc gccgtaccac ttcaaaacac ccaagcacag catactaaat ttcccctctt 300

tcttcctcta gggtgtcgtt aattacccgt actaaaggtt tggaaaagaa aaaagacacc 360

gcctcgtttc tttttcttcg tcgaaaaagg caataaaaat ttttatcacg tttctttttc 420

ttgaaaattt ttttttttga tttttttctc tttcgatgac ctcccattga tatttaagtt 480

aataaacggt cttcaatttc tcaagtttca gtttcatttt tcttgttcta ttacaacttt 540

ttttacttct tgctcattag aaagaaagca tagcaatcta atctaagttt taattacaaa 600

<210> 204

<211> 600

<212> DNA

<213> 人工序列

<220>

<223> Sc Pgk1.启动子

<400> 204

gggccagaaa aaggaagtgt ttccctcctt cttgaattga tgttaccctc ataaagcacg 60

tggcctctta tcgagaaaga aattaccgtc gctcgtgatt tgtttgcaaa aagaacaaaa 120

ctgaaaaaac ccagacacgc tcgacttcct gtcttcctat tgattgcagc ttccaatttc 180

gtcacacaac aaggtcctag cgacggctca caggttttgt aacaagcaat cgaaggttct 240

ggaatggcgg gaaagggttt agtaccacat gctatgatgc ccactgtgat ctccagagca 300

aagttcgttc gatcgtactg ttactctctc tctttcaaac agaattgtcc gaatcgtgtg 360

acaacaacag cctgttctca cacactcttt tcttctaacc aagggggtgg tttagtttag 420

tagaacctcg tgaaacttac atttacatat atataaactt gcataaattg gtcaatgcaa 480

gaaatacata tttggtcttt tctaattcgt agtttttcaa gttcttagat gctttctttt 540

tctctttttt acagatcatc aaggaagtaa ttatctactt tttacaacaa atataaaaca 600

<210> 205

<211> 1001

<212> DNA

<213> 人工序列

<220>

<223> Kl prom 12.pro

<400> 205

cgtaaaaact aaaacgagcc cccaccaaag aacaaaaaag aaggtgctgg gcccccactt 60

tcttcccttg cacgtgatag gaagatggct acagaaacaa gaagatggaa atcgaaggaa 120

agagggagac tggaagctgt aaaaactgaa atgaaaaaaa aaaaaaaaaa aaaaaaacaa 180

gaagctgaaa atggaagact gaaatttgaa aaatggtaaa aaaaaaaaag aaacacgaag 240

ctaaaaacct ggattccatt ttgagaagaa gcaagaaagg taagtatggt aacgaccgta 300

caggcaagcg cgaaggcaaa tggaaaagct ggagtccgga agataatcat ttcatcttct 360

tttgttagaa cagaacagtg gatgtccctc atctcggtaa cgtattgtcc atgccctaga 420

actctctgtc cctaaaaaga ggacaaaaac ccaatggttt ccccagcttc cagtggagcc 480

accgatccca ctggaaacca ctggacagga agagaaaatc acggacttcc tctattgaag 540

gataattcaa cactttcacc agatcccaaa tgtcccgccc ctattcccgt gttccatcac 600

gtaccataac ttaccatttc atcacgttct ctatggcaca ctggtactgc ttcgactgct 660

ttgcttcatc ttctctatgg gccaatgagc taatgagcac aatgtgctgc gaaataaagg 720

gatatctaat ttatattatt acattataat atgtactagt gtggttattg gtaattgtac 780

ttaattttga tatataaagg gtggatcttt ttcattttga atcagaattg gaattgcaac 840

ttgtctcttg tcactattac ttaatagtaa ttatatttct tattaacctt ttttttaagt 900

caaaacacca aggacaagaa ctactcttca aaggtatttc aagttatcat acgtgtcaca 960

cacgcttcac agtttcaagt aaaaaaaaag aatattacac a 1001

<210> 206

<211> 403

<212> DNA

<213> 人工序列

<220>

<223> Ag lox_TEF1.启动子

<400> 206

taccgttcgt ataatgtatg ctatacgaag ttatgtcccc gccgggtcac ccggccagcg 60

acatggaggc ccagaatacc ctccttgaca gtcttgacgt gcgcagctca ggggcatgat 120

gtgactgtcg cccgtacatt tagcccatac atccccatgt ataatcattt gcatccatac 180

attttgatgg ccgcacggcg cgaagcaaaa attacggctc ctcgctgcag acctgcgagc 240

agggaaacgc tcccctcaca gacgcgttga attgtcccca cgccgcgccc ctgtagagaa 300

atataaaagg ttaggatttg ccactgaggt tcttctttca tatacttcct tttaaaatct 360

tgctaggata cagttctcac atcacatccg aacataaaca aca 403

<210> 207

<211> 1000

<212> DNA

<213> 人工序列

<220>

<223> Kl prom 6.pro

<400> 207

caaagggggg gcagggacag ggatacgaca agggctgggg aaaaaaaaaa agatagatac 60

gattggccgg gtaagcctgg ggaaatgtag caagtgcggg taagttaaaa ggtaaccacg 120

tgactccgga agagtcacgt ggttacggac ttttttctct agatctcagc tttttatcgg 180

tcttaccctg ccctcctgcc ccctgcccct tccctttgcc ccaaaaagaa aggaaatctg 240

ttggatttcg ctcaggccat ccctttcgtt aatatcggtt atcgctttac acactgcaca 300

tccttctgtc caaaaggaat ccagaagttt agcttttcct tcctttccca cagacattag 360

cctaggccct ctctcatcat ttgcatgcct cagccaatgt accaagaata acgcaacgag 420

gttgggaaat tttaacccaa caatcgatgc agatgtgaca agagattaga cacgttccag 480

ataccagatt acacagcttg tgctagcaga gtgacatatg gtggtgttgt gtctcgttta 540

gtacctgtaa tcgagagtgt tcaaatcagt cgatttgaac acccttactg ccactgaata 600

ttgattgaat accgtttatt gaaggtttta tgagtgatct tctttcggtc caggacaatt 660

tgttgagctt tttctatgta gagttccgtc cctttttttt ttttttttgc tttctcgcac 720

ttactagcac tatttttttt tcacacacta aaacacttta ttttaatcta tatatatata 780

tatatatata tgtaggaatg gaatcacaga catttgatac tcatcctcat ccttattaat 840

tcttgtttta atttgtttga cttagccaaa ccaccaatct caacccatcg tatttcaggt 900

attgtgtgtc tagtgtgtct ctggtatacg gaaataagtg ccagaagtaa ggaagaaaca 960

aagaacaagt gtctgaatac tactagcctc tcttttcata 1000

<210> 208

<211> 600

<212> DNA

<213> 人工序列

<220>

<223> Sc Pma1.pro

<400> 208

ccatcatgaa aaatctctcg acaccgttta tccattgctt ttttgttgtc tttttccctc 60

gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc 120

cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt 180

tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta 240

tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg 300

tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa 360

caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac 420

ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat 480

tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagctagtta 540

aagaaaatca ttgaaaagaa taagaagata agaaagattt aattatcaaa caatatcaaa 600

<210> 209

<211> 600

<212> DNA

<213> 人工序列

<220>

<223> Sc Vps68.启动子

<400> 209

gatatagccg ctacgactga ttagtgatgt gataaagata gaagatttaa gtcacagagg 60

cgtgcatcta cgattttggc gtttcacatt ttttacactt aaattttagt gatctagccg 120

tgaccttggc agcagtttcc aaaatcattc catgaccatg tcatgcttaa gaacgttaga 180

cccagaacaa gtggacctgt attctaactc ttcactcttg ggcaaagata atagtattat 240

cttttacccc attttttgta tgttttttcg tttattgagt ttggcgtttc ctatttagaa 300

atagtacaat ccggtcaatc attcgatagt gaaatatata tatttaacta ggaaaattag 360

taaaacctca tttaaagatc attcaccttg atatatacta ctattgacct tttgttaatg 420

accattttcg taaaaatgaa ctgcgattct cttctggaat ttgttaccct accttattca 480

ctaaatcaga aataataatg tgcagcgccc ctttcataaa gaaggcaagt atagggcata 540

tagttaaagg tcagaactct ttatccccaa ctacaagatc aattagaaaa tcacatcata 600

<210> 210

<211> 600

<212> DNA

<213> 人工序列

<220>

<223> Sc Oye2.启动子

<400> 210

actacaattt agcggcttag cacaatacgc gttttcaact tcctacgcta gcgatgacaa 60

aatgtctcca agaggcggaa cttgcgacgg atgcatggaa atatcttacg taatgaactt 120

ccgtaatgaa cttccgtaat tcaagatctc ttagcatctc ttgttcaatc ttcagactct 180

actaagtgtt cttaccaacc attggatgct cattacaaat gaatgaatat attgcacgga 240

acggaagcgg catgcttttt ccgtgtcgtg tgcttagtaa agcaaaacgg agtagaatcg 300

gtaagaactt cctttttggg ttggaaaatc attgccattg tttggacacc tttctttttc 360

cgtattgttc gagcaccgcg tttctttttg ggtacttgat gaggtagcag attcctggaa 420

cgtgctttct ctcgaggtaa cctgccttgt tcctcctggt gactttctaa aatataaaag 480

gaaaagcata tctctagttt cgagtttttt cttcatactt tatttcctta tgttaaacgg 540

tccagatata gaataaatca tcatattaag ctaaatatag acgataatat agtatcgata 600

<210> 211

<211> 810

<212> DNA

<213> 人工序列

<220>

<223> KANMX

<400> 211

atgggtaagg aaaagactca cgtttcgagg ccgcgattaa attccaacat ggatgctgat 60

ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga 120

ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc 180

aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg 240

accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc 300

ggcaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat 360

gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac 420

agcgatcgcg tatttcgttt ggctcaggcg caatcacgaa tgaataacgg tttggttgat 480

gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg 540

cataagcttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat 600

aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc 660

gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca 720

ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag 780

tttcatttga tgctcgatga gtttttctaa 810

<210> 212

<211> 301

<212> DNA

<213> 人工序列

<220>

<223> Adh1.终止子

<400> 212

agcgaatttc ttatgattta tgatttttat tattaaataa gttataaaaa aaataagtgt 60

atacaaattt taaagtgact cttaggtttt aaaacgaaaa ttcttattct tgagtaactc 120

tttcctgtag gtcaggttgc tttctcaggt atagcatgag gtcgctctta ttgaccacac 180

ctctaccggc atgccgagca aatgcctgca aatcgctccc catttcaccc aattgtagat 240

atgctaactc cagcaatgag ttgatgaatc tcggtgtgta ttttatgtcc tcagaggaca 300

a 301

<210> 213

<211> 301

<212> DNA

<213> 人工序列

<220>

<223> Adh2.终止子

<400> 213

agcggatctc ttatgtcttt acgatttata gttttcatta tcaagtatgc ctatattagt 60

atatagcatc tttagatgac agtgttcgaa gtttcacgaa taaaagataa tattctactt 120

tttgctccca ccgcgtttgc tagcacgagt gaacaccatc cctcgcctgt gagttgtacc 180

cattcctcta aactgtagac atggtagctt cagcagtgtt cgttatgtac ggcatcctcc 240

aacaaacagt cggttatagt ttgtcctgct cctctgaatc gtgtccctcg atatttctca 300

t 301

<210> 214

<211> 301

<212> DNA

<213> 人工序列

<220>

<223> Gmp1.终止子

<400> 214

agtctgaaga atgaatgatt tgatgatttc tttttccctc catttttctt actgaatata 60

tcaatgatat agacttgtat agtttattat ttcaaattaa gtagctatat atagtcaaga 120

taacgtttgt ttgacacgat tacattattc gtcgacatct tttttcagcc tgtcgtggta 180

gcaatttgag gagtattatt aattgaatag gttcattttg cgctcgcata aacagttttc 240

gtcagggaca gtatgttgga atgagtggta attaatggtg acatgacatg ttatagcaat 300

a 301

<210> 215

<211> 301

<212> DNA

<213> 人工序列

<220>

<223> Sc Tal1.终止子

<400> 215

aggaagtatc tcggaaatat taatttaggc catgtcctta tgcacgtttc ttttgatact 60

tacgggtaca tgtacacaag tatatctata tatataaatt aatgaaaatc ccctatttat 120

atatatgact ttaacgagac agaacagttt tttatttttt atcctatttg atgaatgata 180

cagtttctta ttcacgtgtt atacccacac caaatccaat agcaataccg gccatcacaa 240

tcactgtttc ggcagcccct aagatcagac aaaacatccg gaaccacctt aaatcaacgt 300

c 301

<210> 216

<211> 301

<212> DNA

<213> 人工序列

<220>

<223> Sc Tpi1.终止子

<400> 216

agattaatat aattatataa aaatattatc ttcttttctt tatatctagt gttatgtaaa 60

ataaattgat gactacggaa agctttttta tattgtttct ttttcattct gagccactta 120

aatttcgtga atgttcttgt aagggacggt agatttacaa gtgatacaac aaaaagcaag 180

gcgctttttc taataaaaag aagaaaagca tttaacaatt gaacacctct atatcaacga 240

agaatattac tttgtctcta aatccttgta aaatgtgtac gatctctata tgggttactc 300

a 301

<210> 217

<211> 285

<212> DNA

<213> 人工序列

<220>

<223> Ag Tef1_lox.终止子

<400> 217

atcagtactg acaataaaaa gattcttgtt ttcaagaact tgtcatttgt atagtttttt 60

tatattgtag ttgttctatt ttaatcaaat gttagcgtga tttatatttt ttttcgcctc 120

gacatcatct gcccagatgc gaagttaagt gcgcagaaag taatatcatg cgtcaatcgt 180

atgtgaatgc tggtcgctat actgctgtcg attcgatact aacgccgcca tccagtgtcg 240

aaaacgagct cataacttcg tataatgtat gctatacgaa cggta 285

<210> 218

<211> 301

<212> DNA

<213> 人工序列

<220>

<223> Sc Pdc1.终止子

<400> 218

agcgatttaa tctctaatta ttagttaaag ttttataagc atttttatgt aacgaaaaat 60

aaattggttc atattattac tgcactgtca cttaccatgg aaagaccaga caagaagttg 120

ccgacagtct gttgaattgg cctggttagg cttaagtctg ggtccgcttc tttacaaatt 180

tggagaattt ctcttaaacg atatgtatat tcttttcgtt ggaaaagatg tcttccaaaa 240

aaaaaaccga tgaattagtg gaaccaagga aaaaaaaaga ggtatccttg attaaggaac 300

a 301

<210> 219

<211> 301

<212> DNA

<213> 人工序列

<220>

<223> Sc Tdh1.终止子

<400> 219

aataaagcaa tcttgatgag gataatgatt tttttttgaa tatacataaa tactaccgtt 60

tttctgctag attttgtgaa gacgtaaata agtacatatt actttttaag ccaagacaag 120

attaagcatt aactttaccc ttttctcttc taagtttcaa tactagttat cactgtttaa 180

aagttatggc gagaacgtcg gcggttaaaa tatattaccc tgaacgtggt gaattgaagt 240

tctaggatgg tttaaagatt tttccttttt gggaaataag taaacaatat attgctgcct 300

t 301

<210> 220

<211> 301

<212> DNA

<213> 人工序列

<220>

<223> Sc Eno1.终止子

<400> 220

aagcttttga ttaagccttc tagtccaaaa aacacgtttt tttgtcattt atttcatttt 60

cttagaatag tttagtttat tcattttata gtcacgaatg ttttatgatt ctatataggg 120

ttgcaaacaa gcatttttca ttttatgtta aaacaatttc aggtttacct tttattctgc 180

ttgtggtgac gcgtgtatcc gcccgctctt ttggtcaccc atgtatttaa ttgcataaat 240

aattcttaaa agtggagcta gtctatttct atttacatac ctctcatttc tcatttcctc 300

c 301

<210> 221

<211> 1000

<212> DNA

<213> 人工序列

<220>

<223> Kl prom 3.pro

<400> 221

gagcctgtcc aagcaaatgc cttctcataa atggtgccaa agacccgcaa gcccaaagca 60

attacccccc aaaaagaaat gatatagtgc aagatacgta tatgaccatg acttgactag 120

gtgaaacagt gcagaaacag ccgcacaaaa gcagccctaa ccctcagagt cgattttact 180

ctttcaggta ataaagcctc gacatcaatt ttagacagaa gccaggctgg cctcgagatt 240

atagccatag gcaagcaaga ggagagaagg ggaggccccc catggggggc ctcccccccg 300

ctgtcaaggt ttggcagaac ctagcttcat taggccacta gcccagccta aaacgtcaac 360

gggcaggagg aacactccca caagacggcg tagtattctc gattcataac cattttctca 420

atcgaattac acagaacaca ccgtacaaac ctctctatca taactactta atagtcacac 480

acgtactcgt ctaaatacac atcatcgtcc tacaagttca tcaaagtgtt ggacagacaa 540

ctataccagc atggatctct tgtatcggtt cttttctccc gctctctcgc aataacaatg 600

aacactgggt caatcatagc ctacacaggt gaacagagta gcgtttatac agggtttata 660

cggtgattcc tacggcaaaa atttttcatt tctaaaaaaa aaaagaaaaa tttttctttc 720

caacgctaga aggaaaagaa aaatctaatt aaattgattt ggtgattttc tgagagttcc 780

ctttttcata tatcgaattt tgaatataaa aggagatcga aaaaattttt ctattcaatc 840

tgttttctgg ttttatttga tagttttttt gtgtattatt attatggatt agtactggtt 900

tatatgggtt tttctgtata acttcttttt attttagttt gtttaatctt attttgagtt 960

acattatagt tccctaactg caagagaagt aacattaaaa 1000

<210> 222

<211> 1000

<212> DNA

<213> 人工序列

<220>

<223> Kl prom 2.pro

<400> 222

gagcctgtcc aagcaaatgc cttctcataa atggtgccaa agacccgcaa gcccaaagca 60

attacccccc aaaaagaaat gatatagtgc aagatacgta tatgaccatg acttgactag 120

gtgaaacagt gcagaaacag ccgcacaaaa gcagccctaa ccctcagagt cgattttact 180

ctttcaggta ataaagcctc gacatcaatt ttagacagaa gccaggctgg cctcgagatt 240

atagccatag gcaagcaaga ggagagaagg ggaggccccc catggggggc ctcccccccg 300

ctgtcaaggt ttggcagaac ctagcttcat taggccacta gcccagccta aaacgtcaac 360

gggcaggagg aacactccca caagacggcg tagtattctc gattcataac cattttctca 420

atcgaattac acagaacaca ccgtacaaac ctctctatca taactactta atagtcacac 480

acgtactcgt ctaaatacac atcatcgtcc tacaagttca tcaaagtgtt ggacagacaa 540

ctataccagc atggatctct tgtatcggtt cttttctccc gctctctcgc aataacaatg 600

aacactgggt caatcatagc ctacacaggt gaacagagta gcgtttatac agggtttata 660

cggtgattcc tacggcaaaa atttttcatt tctaaaaaaa aaaagaaaaa tttttctttc 720

caacgctaga aggaaaagaa aaatctaatt aaattgattt ggtgattttc tgagagttcc 780

ctttttcata tatcgaattt tgaatataaa aggagatcga aaaaattttt ctattcaatc 840

tgttttctgg ttttatttga tagttttttt gtgtattatt attatggatt agtactggtt 900

tatatgggtt tttctgtata acttcttttt attttagttt gtttaatctt attttgagtt 960

acattatagt tccctaactg caagagaagt aacattaaaa 1000

<210> 223

<211> 600

<212> DNA

<213> 人工序列

<220>

<223> Sc PRE3.启动子

<400> 223

caaacattaa tttgttctgc atactttgaa cctttcagaa aataaaaaac attacgcgca 60

tacttaccct gctcgcgaag aagagtaaca ctaacgcatt ctatgggcaa ttgaagacag 120

tattcagtac aagacatagt ccgtttcctt gagtcaattc ctatagcatt atgaactagc 180

cgcctttaag agtgccaagc tgttcaacac cgatcatttt tgatgatttg gcgtttttgt 240

tatattgata gatttctttt gaattttgtc attttcactt ttccactcgc aacggaatcc 300

ggtggcaaaa aagggaaaag cattgaaatg caatctttaa cagtatttta aacaagttgc 360

gacacggtgt acaattacga taagaattgc tacttcaaag tacacacaga aagttaacat 420

gaatggaatt caagtggaca tcaatcgttt gaaaaagggc gaagtcagtt taggtacctc 480

aatgtatgta tataagaatt tttcctccca ctttattgtt tctaaaagtt caatgaagta 540

aagtctcaat tggccttatt actaactaat aggtatctta taatcaccta ataaaataga 600

Claims (15)

1.一种具有式(I)的甜菊醇糖苷，

其中在位置R₁上存在至少三个糖部分且在位置R₂上存在至少三个糖部分，且其中所述甜菊醇糖苷包括至少七个糖部分，所述至少七个糖部分全部均直接或间接地通过β键联接到甜菊醇糖苷配基。

2.一种具有式(I)的甜菊醇糖苷，

其中在位置R₁上存在至少四个糖部分且在位置R₂上存在至少三个糖部分。

3.一种具有式(I)的甜菊醇糖苷，

其中在位置R₁上存在至少三个糖部分且在位置R₂上存在至少三个糖部分，其中所述甜菊醇糖苷包括至少七个糖部分，且其中存在于位置R₁的糖中的至少一个通过α键被联接到甜菊醇糖苷配基或糖分子。

4.一种具有式(I)的甜菊醇糖苷，

其中在位置R₁上存在至少三个糖部分且在位置R₂上存在至少四个糖部分，其中存在于位置R₂上的所述糖部分中的至少四个为葡萄糖部分。

5.一种具有式(II)的甜菊醇糖苷

6.一种具有式(III)的甜菊醇糖苷

7.一种具有式(IV)的甜菊醇糖苷

8.根据前述权利要求中任一项所述的甜菊醇糖苷，其为发酵制备的。

9.一种发酵制备的具有式(I)的甜菊醇糖苷，

其中在位置R₁上存在至少三个糖部分且在位置R₂上存在至少三个糖部分，且其中所述甜菊醇糖苷包括至少七个糖部分。

10.根据权利要求9所述的甜菊醇糖苷，其具有根据权利要求1至7中的任一项所述的结构。

11.一种用于制备根据前述权利要求中任一项所述的甜菊醇糖苷的方法，所述方法包括：

提供包含编码多肽的重组核酸序列的重组酵母细胞，所述多肽包含由下列编码的氨基酸序列：SEQ ID NO:61、SEQ ID NO:65、SEQ ID NO:23、SEQ ID NO:33、SEQ ID NO:77、SEQID NO:71、SEQ ID NO:87、SEQ ID NO:73和SEQ ID NO:75；

在合适的发酵培养基中发酵所述重组酵母细胞；以及，可选地，

回收根据前述权利要求中任一项所述的甜菊醇糖苷。

12.一种组合物，其包括根据权利要求1至11中的任一项所述的甜菊醇糖苷以及一种或更多种不同的甜菊醇糖苷。

13.一种食品、饲料或饮料，其包括根据权利要求1至10中的任一项所述的甜菊醇糖苷或根据权利要求12所述的组合物。

14.根据权利要求1至10中的任一项所述的甜菊醇糖苷或根据权利要求12所述的组合物在甜味剂组合物或风味组合物中的用途。

15.根据权利要求1至10中的任一项所述的甜菊醇糖苷或根据权利要求12所述的组合物在食品、饲料或饮料中的用途。

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