CN113303115A - Pinus kesiya cutting propagation seedling raising method - Google Patents
Pinus kesiya cutting propagation seedling raising method Download PDFInfo
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- CN113303115A CN113303115A CN202110737204.3A CN202110737204A CN113303115A CN 113303115 A CN113303115 A CN 113303115A CN 202110737204 A CN202110737204 A CN 202110737204A CN 113303115 A CN113303115 A CN 113303115A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/22—Improving land use; Improving water use or availability; Controlling erosion
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/40—Afforestation or reforestation
Abstract
The invention discloses a cutting propagation seedling raising method for pinus khasys, which comprises four steps of cutting seedling raising, management of cutting seedlings, seedling hardening and outplanting and afforestation. The invention not only improves the rooting rate of the cutting seedling of the pinus khasys by applying the microbial inoculum, but also has no obvious seedling recovering stage in the seedling stage, the microbial inoculum is rich in nutrients required by the growth of the seedling, and is also rich in bacillus, so that the absorption of the plant to nutrient elements can be promoted, and the growth of the plant can be indirectly promoted.
Description
Technical Field
The invention relates to the technical field of cutting seedling raising, in particular to a pinus khasys cutting propagation seedling raising method.
Background
The pinus khasys (Pinus khesyavar. langbianensis) is a geographical variety of Pinus khasys (Pinus khesyas), has the characteristics of fast growth, high lipid yield, strong ecological adaptability and the like, is an important tree species for both wood and lipid in Yunnan province, is mainly distributed in Puer cities, Lincang regions, Jinghong cities and partial counties of the state of the red river in Yunnan province, and has the altitude distribution range of 700-1800 m.
The pinus khasys is not only an important commercial forest source tree species, but also an afforestation tree species with the largest construction area of an artificial forest. In recent years, forestry workers pay attention to the method for cultivating pinus khasys seedlings by adopting asexual cutting propagation, a great deal of work is done in the research of pinus khasys cutting technology, the work comprises the selection and treatment of scion wood, the selection of cutting medium and environment, the tests of the influence of microbial fertilizer on the rooting rate of pinus khasys cutting and the growth of cutting seedlings, and the like, and the cutting technology is relatively mature.
However, after the pinus khasys forestation, the pinus khasys grows slowly in 5 months, and the forestation effect is seriously influenced, so that how to further supplement the methods for strong seedling cultivation in the cutting seedling stage and the growth amount in the initial forestation stage of the pinus khasys, and provide new technical measures for the strong seedling cultivation of the cutting seedling stage of the pinus khasys, are technical problems to be solved urgently by technical personnel in the field.
Disclosure of Invention
In view of the above, the invention provides a pinus khasys cutting propagation seedling raising method which can greatly improve the survival rate of cutting seedlings and can improve the afforestation growth amount of pinus khasys.
In order to achieve the purpose, the invention adopts the following technical scheme:
a cutting propagation seedling raising method for pinus khasys comprises the following steps:
(1) cutting seedling raising
Cutting and seedling by using mountain red soil as a matrix, selecting the scion cutting stock plants to germinate semi-lignified crushed aggregates in the current year, soaking the scion cutting in a treatment solution for 3-5s, cutting into the matrix, compacting the matrix around the scion cutting, watering thoroughly, immediately building an arch shed above the cutting plants after cutting, and covering a shading net with shading rate of 75% on the arch shed in an arch manner to form the shading shed;
(2) management of cutting seedlings
After cutting of the scions, spraying clear water on leaf surfaces every morning, spraying clear water on leaf surfaces every 3d after 20d, spraying a bactericide on leaf surfaces every 7d, spraying a chemical fertilizer on leaf surfaces every 15d, applying a 4000-fold liquid of a microbial agent every 30d, and stopping fertilizing one month before outplanting;
(3) hardening off seedlings
Cutting the scion wood for 85-95 days, opening two ends of the arched shed, ventilating, keeping for 8-12 days, then completely uncovering the arched shed, keeping for 18-22 days, and finally completely uncovering the shade shed, and performing full-light seedling hardening;
(4) afforestation out of nursery
In the planting and afforestation process, the row spacing of planted plants is selected to be 3m multiplied by 3m, the planted plants are thoroughly chopped and nurtured once in 11-12 months in the first year after afforestation, the planted plants are thoroughly chopped and nurtured twice in 4-5 months and 11 months in the second year, combined soil loosening and nurturing is carried out in 4-5 months, each plant is applied with 100 plus materials of compound fertilizer and 200g, young pinus khasys forests in the third year are gradually closed, and the planted plants are thoroughly chopped and nurtured once in 4-5 months.
Preferably, the seedling raising container for performing cutting seedling raising in the step (1) is a non-woven fabric nutrition bag with the specification of 10 x 12 cm.
Preferably, the treatment solution in the step (1) is 4000 times of biological agent and ABT with the concentration of 1000mg/L1The ear strips are respectively put in 4000 times of biological agent solution and ABT with the concentration of 1000mg/L1Soaking for 3-5 s.
The biological agent used in the invention is a RhizovitalRfl microbial fertilizer agent produced by Yunnan Reynaud Exie Biotech limited, is rich in bacillus, the number of effective viable bacteria is more than or equal to 0.5 hundred million/g, the treatment solution not only improves the rooting rate of the cutting seedlings of the pinus khasys, but also has no obvious seedling recovering period in the seedling stage, and the fertilizer is rich in nutrients required by the growth of the seedlings, and also is rich in bacillus, thereby not only promoting the absorption of the plants to the nutrient elements, but also indirectly promoting the growth of the plants.
Preferably, the humidity in the greenhouse is kept to be more than 90% within 1 month after the cutting of the scion and the spike in the step (2), and the humidity in the greenhouse is kept to be more than 80% after 1 month.
Preferably, the bactericide in the step (2) is 800 times of carbendazim liquid or 1000 times of methyl mop liquid; the fertilizer is urea with the concentration of 1%.
In the setting, the bactericide can prevent and treat root rot, improve the survival rate of cuttage, and the fertilizer can improve the growth of seedlings.
Preferably, the moldy ear is timely treated during the management of the cutting seedlings, and 1500 times of liquid of metalaxyl is selected for prevention and treatment.
Preferably, the slow release fertilizer is applied after the full-light seedling refining in the step (3), the application amount of the slow release fertilizer is 3 g/plant, and the slow release fertilizer is N: p2O5：K2The mass ratio of O is 16:9: 12.
The slow release fertilizer can improve the growth amount of cutting seedlings of pinus khasys, and has a lasting effect of 7-8 months after afforestation due to long fertilizer efficiency period.
Preferably, the land is completely cleaned before forestation in the step (4), then a hole-shaped land preparation is adopted, a planting hole is dug along a contour line, the specification is 40cm × 40cm × 30cm, surface soil and subsoil are respectively placed on one side of the hole during hole digging, the surface soil is returned firstly when the pond returns, then the subsoil is returned, a small hole of 20cm × 20cm is dug in the middle after the soil is returned, seedlings are placed in the small hole, the soil is surrounded and compacted, then the soil is covered into a steamed bun shape, and the situation that the base part is buried in the soil is avoided.
Preferably, the compound fertilizer in the step (4) is N: p2O5：K2The mass ratio of O is 15:15: 15.
Through the technical scheme, compared with the prior art, the invention has the following beneficial effects:
(1) the invention not only improves the rooting rate of the cutting seedling of pinus khasys by applying the microbial agent, but also has no obvious seedling revival stage in the seedling stage, and the fertilizer is rich in nutrients required by seedling growth and also rich in bacillus, thereby not only promoting the absorption of the plant to the nutrient elements, but also indirectly promoting the growth of the plant;
(2) the slow release fertilizer and the application amount of 3 g/plant are selected, the growth amount of the pinus khasys cutting seedlings in the seedling stage and after afforestation is better than other usage amount, and the slow release fertilizer has longer effective period and can last for 3-5 months after afforestation, thereby effectively promoting the growth of the pinus khasys cutting seedlings after afforestation;
(3) the biological agent and the slow release fertilizer are used in a matched manner, so that the survival rate of the cutting seedlings is greatly improved, the biological agent has a remarkable effect on promoting growth of pinus khasys in the cutting seedling stage and 1 year after afforestation, the productivity can be greatly improved when the biological agent is applied to production, and the biological agent has an important significance on the development of pinus khasys industry.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A cutting propagation seedling raising method for pinus khasys comprises the following steps:
(1) cutting seedling raising
Cutting and raising seedlings by using mountain red soil as a matrix, wherein a seedling raising container is a non-woven fabric nutrition bag of 10 multiplied by 12cm, the current-year half-lignified lateral branches of a scion picking stock plant are selected to sprout, the length of the scion is about 10cm, the scion is dipped in treatment liquid quickly for 3-5s and then is inserted into the matrix, the cutting depth of the scion is 1-3cm, the matrix around the scion is compacted, the matrix is watered thoroughly to keep the matrix moist, a plastic arched shed is built immediately above the cutting plant after the cutting is finished, and a shading net with the shading rate of 75% is arched on the arched shed to form a shading shed;
(2) management of cutting seedlings
After cutting of the scion wood, keeping the humidity in the greenhouse to be more than 90% within 1 month, keeping the humidity in the greenhouse to be more than 80% after 1 month, spraying clear water on leaf surfaces every morning, spraying clear water on leaf surfaces every 3dd after 20 days, spraying carbendazim 800 times liquid on leaf surfaces every 7dd, spraying urea with a concentration of 1% on leaf surfaces every 15d, and stopping fertilizing one month before outplanting; the mildew ears are found to be treated in time, and 1500 times of liquid of metalaxyl is selected for prevention and treatment;
(3) hardening off seedlings
Cutting the scion wood for 85-95 days, opening plastic films at two ends of the arched shed, ventilating, keeping for 8-12 days, then completely uncovering the arched shed, keeping for 18-22 days, and finally completely uncovering the shading shed, and performing full-light seedling hardening;
(4) afforestation out of nursery
Cleaning soil, preparing soil in cave shape, digging planting holes along contour line with specification of 40cm × 40cm × 30cm, placing surface soil and subsoil on one side of the holes, and returning surface soil to pondThen the subsoil is returned. Digging a small hole of 20cm multiplied by 20cm in the center after soil is returned, putting the nursery stock into the hole, compacting by treading the soil, covering soil into a steamed bread shape, avoiding the pine needles at the base part from being buried in the soil, ensuring the row spacing of the planted nursery stock to be 3m multiplied by 3m, completely cutting grass for the afforestation site once in the first 11-12 months after afforestation, completely cutting grass for the afforestation site twice in the second 4-5 months and 11 months, combining with loosening soil for nurturing in the second 4-5 months, and applying a compound fertilizer (N: P) to each nursery stock2O5:K2O15: 15:15)100 and 200g, gradually closing young pinus khasys in the third year, and cutting grass for one time in 4-5 months.
In this example, the treatment solution was 4000-fold biological agent solution and ABT with a concentration of 1000mg/L1(ii) a The ear strips are respectively in 4000 times of biological agent solution and ABT with the concentration of 1000mg/L1Soaking for 3-5 s.
In this example, the slow release fertilizer N, P2O5And K2The mass ratio of O is 16:9:12, and the application amount is 3 g/plant.
Example 2
Through setting up the experiment research influence of different factors to pine khasys cuttage propagation seedling raising
1 general description of test region
1.1 overview of the Strong seedling cultivation test area
The test is carried out in a nursery garden of a scientific research institute of forestry in Puer city, the geographical positions of the test are 99 degrees 09'E and 22 degrees 05' N, the altitude is 1300m, the test belongs to subtropical monsoon climate, dry and wet seasons are obvious, dry seasons are from 11 months to 4 months next year, and rainy seasons are from 5 months to 10 months; the annual average temperature is 19.4 ℃, the accumulated temperature is more than or equal to 10 ℃ and 6302 ℃, the air temperature is poor in a year and big in a day, the radiation fog is generated in winter and spring, the foggy day is 138d every year, and the annual precipitation is 1340.9 mm; the soil belongs to acid red soil, and the pH value is 4.6-6.3.
1.2 overview of the test area for forestation
The afforestation land belongs to a pinus khasys cutover area, is located in a Meng town of Yun town Yun county of Yunnan province, is located between 100 degrees of 39 'to 100 degrees of 56' E and 23 degrees of 54 'to 24 degrees of 08' N, belongs to semihumid monsoon climate of the subtropical zone of south China, and has the remarkable characteristics of rain and heat in the same season, clear dry and wet seasons, no summer heat, no severe cold in winter and sufficient light energy and heat. Due to the complex plateau mountain landform, the horizontal terrain regularity is influenced, the vertical change of the climate is obvious, and the three-dimensional climate has the characteristics of small annual temperature difference and large daily temperature difference. The average temperature is 18.9 ℃, the annual rainfall is 1300-1700 mm, the annual average relative temperature is 78%, and the accumulated temperature is 6017.5-7219 ℃ which is not lower than 10 ℃. The elevation of the implementation land is 1350-1600 m, the soil is red soil, and the implementation land is a typical pinus khasys suitable growing area.
2 materials and methods
2.1 test materials
The scion used in the test is seeds collected from a clonal seed garden of pinus khasys of a scenic valley demonstration forest farm in 2018 and 1 month, after 3 months of seedling raising, 6 months of separation and strong seedling are planted as a scion stock plant, and the scion used for cuttage is collected from lateral branches where the scion stock plant sprouts; the seedling raising container is a non-woven fabric nutrition bag with the specification of 10cm multiplied by 12 cm; the substrate is mountain red loam; the microbial agent is a microbial fertilizer agent of Rhizovitalrfl produced by Yunnan Reynaud EbBiotechnology Limited, is rich in bacillus, has effective viable count of more than or equal to 0.5 hundred million/g, and is applied slowly with N: p2O5：K2O16: 9:12, 8-9 months of fertilizer efficiency.
2.2 test methods
2.2.1 study of the Effect of applying microbial Agents and Slow Fertilizer on the cultivation of Strong seedlings of pinus khasys
Adopts three factors of different hormone formulas (A), the application times (B) of the biological agent and the application amount (C) of the slow release fertilizer, and adopts orthogonal test design L9(34) The influence of different factors on the cultivation of the strong cutting seedlings of the pinus khasys is researched, and the experimental design is shown in tables 1 and 2. The method comprises the following steps of cutting 100 cuttings in each treatment, wherein the total number of cuttings is 2700, applying biological fungicide after cutting, using 4000 times of liquid each time, slowly applying fertilizer after cutting (about 120 d) and full-light hardening, and cutting the pinus khasys in 3 different treatment solutions after dipping roots, wherein the experimental design is shown in tables 1 and 2.
TABLE 1 three-factor three-level table
TABLE 2 orthogonal experimental design Table
The pinus khasys spikes are dipped in 3 different treatment solutions and then subjected to cuttage, the biological agent is applied after cuttage, the application concentration is 4000 times of the solution, and the slow release fertilizer is applied after the cuttage seedlings enter full-light hardening (about 120 days). Each treatment is performed by 100 cuttings, and the process is repeated for 3 times, wherein 2700 cuttings are needed.
2.2.2 Afforestation test
And 7, in 2019, hanging and transporting the nursery stock hanging labels of each treatment experiment in the seedling raising experiment to an afforestation site, setting 3 times of repetition by adopting single-factor experiment design of a seedling stage in the experiment, arranging the nursery stock hanging labels from the top of a mountain to the feet of the mountain in an upper, middle and lower mode, wherein each block comprises all 6 treatments, 15 plants are fixedly planted in each treatment, 270 plants are counted, and a protection row is arranged at the edge of the whole afforestation test site.
2.3 index determination and data analysis
And (4) surveying the rooting and survival rate in outplanting at 4 months in 2020, and the height, the ground diameter, the root length, the number of lateral roots and the outplanting rate in outplanting at 7 months. And (4) surveying the height and the ground diameter of the seedlings in 1 year of afforestation, analyzing data by adopting SPSS software, and performing multiple comparison by using an LSD method.
3 results and analysis
3.1 study of the Effect of applying microbial Agents and Slow Fertilizer on the cultivation of Strong seedlings of pinus khasys
3.1.1 survival rate of cutting seedlings of pinus khasys
And (4) surveying the survival rate of the cutting seedlings of the pinus khasys after 120d of cutting, performing arcsine conversion on the survival rate, and performing variance analysis, wherein the results are shown in a table 3.
TABLE 3 examination of the Effect between subjects
a.R Fang ═ 0.959 (adjustment R Fang ═ 0.947)
The results of the anova show that different hormone formulas have extremely obvious influence on the survival rate of the cutting seedlings of the pinus khasys (Sig. ═ 0.000<0.01), and the application times of different biological inoculants have no obvious influence on the survival rate of the cutting seedlings (Sig. ═ 0.508> 0.01). Multiple comparisons of survival rates were made and the results are shown in table 4.
TABLE 4 multiple comparison of survival rates
The results in table 4 show that the rooting agent and biological agent combined treatment is carried out on the pinus khasys cuttings during cuttage, the survival rate of the cuttage seedlings can reach 93.07%, the survival rate is obviously higher than that of the cuttage seedlings obtained by singly using the rooting agent or the biological agent, and the combination formula of the rooting agent and the biological agent has an extremely obvious promotion effect on the survival of the pinus khasys cuttage seedlings and can be popularized and used in the production of the pinus khasys cuttage seedlings.
3.1.2 Effect of different factors on the growth of cutting seedlings of pinus khasys
After cutting seedlings enter full-light hardening (about 120 days), slow release fertilizer is applied, indexes such as the nursery rate, the seedling height, the ground diameter, the number of roots and the root length of the cutting seedlings are investigated when the cutting seedlings are out of the nursery, and the influence of combined treatment of 3 factors of different hormone formulas, the application times of biological microbial agents and the application amount of the slow release fertilizer on the growth of the cutting seedlings of pinus khasys is explored.
(1) Influence of different factors on outplanting rate of pinus khasys cutting seedlings
The nursery rate of the pinus khasys cutting seedlings is investigated when the pinus khasys cutting seedlings are out of nursery, the nursery rate is subjected to arcsine conversion and then variance analysis is carried out, and the results are shown in table 5.
TABLE 5 examination of the Effect between subjects
a.R Fang (0.971) ("R Fang
The results in table 5 show that different hormone formulations and slow release fertilizer application amounts all have very significant effects on the cutting seedling outplanting rate (sig. ═ 0.000<0.01), and the application times of the biological fungicide have little effect on the outplanting rate (sig. ═ 0.403> 0.05). Multiple comparisons of out-of-nursery rates were made and the results are shown in table 6.
TABLE 6 multiple comparison of the Effect of different factors on the outplanting rate of Pinus khasys cutting seedlings
Multiple comparison results show that in different hormone formulations, ABT1The combined formula of 1000mg/L plus 4000 times of biological agent can obviously improve the outplanting rate of the pinus khasys cutting seedlings; in the application amount of the slow release fertilizer, when the using amount of the slow release fertilizer reaches 5 g/plant, the outplanting rate of the cutting seedlings can be obviously reduced, and practical investigation also shows that when the using amount of the slow release fertilizer is 5 g/plant, the cutting seedlings can be burnt, so that the using amount of the slow release fertilizer of the pinus khasys cutting seedlings cannot exceed 3 g/plant.
When indexes such as seedling height, ground diameter, root number, root length and the like are analyzed, the slow release fertilizer is applied in an amount of 5 g/plant to cause seedling burning and cannot be used in production, so that the group of data is meaningless for production, and the data of 5 g/plant is removed in the following analysis.
(2) Influence on seedling height
The results of the test were analyzed for variance and are shown in Table 7.
TABLE 7 examination of the Effect between subjects
a.R Fang-0.934 (adjusting R Fang-0.905)
The results in table 7 show that different hormone formulations, the application times of the biological agent and the application amount of the slow release fertilizer have very significant influence on the height of the cutting seedlings (Sig is 0.000< 0.01). Multiple comparisons of seedling height were made and the results are shown in table 8.
TABLE 8 multiple comparison of high Effect of different factors on Pinus khasys cutting seedlings
Multiple comparison results show that in different hormone formulations, ABT1The 1000mg/L + 4000 times liquid of the biological agent and the 4000 times liquid of the biological agent have obvious promotion effects on the high growth of seedlings, and in the application times of the biological agent, the biological agent is not applied and has very obvious difference with the biological agent, so that the biological agent has obvious promotion effects on the growth of the cutting seedlings of pinus khasys. The multiple comparison result also shows that the high growth of the cutting seedlings can be promoted when the application amount of the slow release fertilizer is 3 g/plant.
(3) Influence on the ground diameter
The results of the test were analyzed for variance and are shown in Table 9.
TABLE 9 examination of the Effect between subjects
a.R20.884 (adjustment of R)2＝0.833)
Different formulas, the application times of the biological bactericide and the application amount of the slow release fertilizer have extremely obvious influence on the ground diameter of the cutting seedlings (Sig is 0.000< 0.01). Multiple comparisons of seedling heights were made and the results are shown in table 10.
TABLE 10 multiple comparison of the effects of different factors on the diameter of Pinus khasys cutting seedlings
Table 10 results show that ABT was used1The pine khasys cutting seedling is treated by 1000mg/L + 4000 times liquid of biological microbial inoculum or 4000 times liquid of biological microbial inoculum, then cuttage is carried out, the biological microbial inoculum is applied after cuttage, and 3 g/plant of slow release fertilizer is applied after the survival of the cuttage seedling, so that the growth of the ground diameter of the cuttage seedling of the pine khasys can be remarkably promoted.
(4) Influence on rooting quantity of pinus khasys cutting seedlings
The results of the test were analyzed for variance and are shown in Table 11.
TABLE 11 examination of the effects between subjects
a.R Fang (0.876 (adjustment R Fang (0.821))
The results of the anova show that different formulas have obvious influence on the rooting number of the cutting seedlings of pinus sylvestris (Sig. ═ 0.022<0.05), the application amount of the slow release fertilizer has extremely obvious influence on the rooting number of the cutting seedlings (Sig. ═ 0.000<0.01), and the application times of the biological bactericide has no influence on the roots (Sig. ═ 0.059> 0.05).
The results show that treatment group A2B1C2The rooting quantity of the cutting seedlings is obviously lower than that of other treatment groups, the analysis result of variance shows that the application times of the biological agent has no obvious influence on the lateral root germination, and only A exists in the process of the growth of the cutting seedlings of pinus khasys2B1C2The treatment group does not use a biological agent, which shows that the biological agent has a remarkable promoting effect on the lateral root germination of the pinus khasys cutting seedlings and is irrelevant to the application times. Multiple comparisons of the number of roots were made and the results are shown in Table 12.
TABLE 12 multiple comparison of the effects of different factors on the number of rooting of Pinus khasys cuttings
Multiple comparison results show that different hormone formulations are ABT1The seed number of 1000mg/L + 4000 times of biological agent or 4000 times of biological agent, and 3 g/plant of slow release fertilizer is applied after the cutting seedling survives, which can obviously promote the germination of lateral roots of the cutting seedling of pinus khasys.
(5) Influence on root length
The results of the test were analyzed for variance and are shown in Table 13.
TABLE 13 examination of the effects between subjects
a.R Fang (0.900 Fang (0.855 regulating R Fang)
The results in table 13 show that different formulations, the application times of the biological agent and the application amount of the slow release fertilizer have very significant influence on the growth of the lateral roots of the cutting seedlings (sig. ═ 0.000< 0.01). Multiple comparisons of root length were made and the results are shown in Table 14.
TABLE 14 multiple comparison of the effects of different factors on the root length of Pinus khasys cutting seedlings
The results in Table 14 show that ABT is present in different hormone formulations1The 1000mg/L plus 4000 times of biological agent or 4000 times of biological agent can promote the growth of lateral roots of cutting seedlings of pinus khasys; in the application times of the biological agent, the biological agent is not applied and has very obvious difference with the biological agent, which shows that the biological agent has obvious promotion effect on the growth of lateral roots of the pinus khasys cutting seedlings; in the application amount of the slow release fertilizer, when the application amount of the slow release fertilizer is 3 g/plant, the lateral roots of the pinus khasys grow best.
To sum up, ABT is used when the pinus khasys ear is stripped1Combined formula solution of No. 1000mg/L + 4000 times liquid of biological agentThe liquid dips in roots and then is used for cuttage, the survival rate of cuttage seedlings can be remarkably improved, and biological agents are applied after cuttage, and 3 g/plant slow release fertilizer is applied after the cuttage seedlings survive, so that the growth of the cuttage seedlings can be remarkably promoted. The test result also shows that the effect of simultaneously applying the biological agent and the slow release fertilizer is more obvious than that of singly applying the biological agent or the slow release fertilizer, and the biological agent and the slow release fertilizer can generate obvious interaction to jointly promote the growth of the pinus khasys cutting seedlings.
Combining all test results to screen out the best combination A1B2C2。
3.2 Afforestation test
According to the test results of strong seedling cultivation in seedling stage, 5 treatment combinations, namely A, are screened from the table 21B2C2、A3B1C3、A3B3C2、A2B1C2、A2B3C3(control), treatment combination A1B2C2：ABT1No. 1000mg/L + 4000 times of biological agent, 1 time of biological agent application and 3g of slow fertilization application amount per plant; treatment combination A3B1C3: 4000 times of liquid of the biological agent, 2 times of application of the biological agent and 3g of slow fertilization application amount per plant; treatment combination A3B3C2: biological agent 4000 times liquid, 1 time of biological agent application, 1g of slow fertilization application amount per plant and treatment combination A2B1C2：ABT1Number 1000mg/L + biological agent application times 2 times + slow fertilization application amount 5 g/plant; treatment combination A2B3C3(control): ABT1No. 1000mg/L + slow fertilization application amount 3 g/plant. The growth amount was investigated 1 year after forestation. Variance analysis is carried out on the height and the ground diameter of the seedlings, and the results are shown in a table 15.
TABLE 15 one-way analysis of variance
The result shows that the cutting seedlings of the pinus sylvestris treated by different treatments are afforested for 1 year, and the height and the ground diameter of the seedlings are very different (Sig. ═ 0.000< 0.01). Multiple comparisons of seedling heights were made and the results are shown in table 16.
TABLE 16 multiple comparison of seedling heights
The results show that the biological agent and the slow release fertilizer have very obvious difference compared with the ground diameter control on the height of the seedlings when the pinus khasys cutting seedlings are planted for 1 year, the formula of the rooting agent contains the biological agent, the slow growth stage does not exist after the treatment afforestation by applying the bacterial fertilizer every month, the biological agent and the slow release fertilizer are applied for 3g, the growth amount is obviously higher than that of other treatments, the average seedling heights of the best treatment combination 2 and the best treatment combination 7 respectively reach 117.1cm and 112.1cm, are 145 percent and 134 percent higher than the average seedling height of 47.9cm of 6 treated by the control, the average ground diameter is 33.0mm and 31.8mm respectively, and are 110 percent and 103 percent higher than the average ground diameter of 15.7mm of 6 treated by the control. Treatment 6 (control) no biological agent is applied from the cutting seedling raising formula and the seedling stage, the growth is slow in 5 months after afforestation, and the seedling height and the ground diameter are lower than those of other treatments.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other. The device disclosed by the embodiment corresponds to the method disclosed by the embodiment, so that the description is simple, and the relevant points can be referred to the method part for description.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (9)
1. A cutting propagation seedling raising method for pinus kesiya is characterized by comprising the following steps:
(1) cutting seedling raising
Cutting and seedling by using mountain red soil as a matrix, selecting the scion cutting stock plant to germinate semi-lignified lateral branches in the current year, soaking the scion in a treatment solution for 3-5s, then cutting into the matrix, compacting the matrix around the scion and watering thoroughly, immediately building an arch shed above the cutting plant after cutting, and covering a shading net with shading rate of 75% on the arch shed to form the shading shed;
(2) management of cutting seedlings
After cutting of the scions, spraying clear water on leaf surfaces every morning, spraying clear water on leaf surfaces every 3d after 20d, spraying a bactericide on leaf surfaces every 7d, spraying a chemical fertilizer on leaf surfaces every 15d, applying a 4000-fold liquid of a microbial agent every 30d, and stopping fertilizing one month before outplanting;
(3) hardening off seedlings
Cutting the scion wood for 85-95 days, opening two ends of the arched shed, ventilating, keeping for 8-12 days, then completely uncovering the arched shed, keeping for 18-22 days, and finally completely uncovering the shade shed, and performing full-light seedling hardening;
(4) afforestation out of nursery
In the planting and afforestation process, the row spacing of planting plants is selected to be 3m multiplied by 3m, the grass is fully cut and nursed once in the first 11-12 months after afforestation, the grass is fully cut and nursed twice in the second 4-5 months and the second 11 months, soil loosening and nurturing are combined in the 4-5 months, each plant is applied with 100 plus materials of compound fertilizer 200g, the young pinus khasys forest in the third year is gradually closed, and the grass is cut and nursed once in the 4-5 months.
2. The cuttage propagation seedling raising method for pinus khasys according to claim 1, wherein the length of the scion wood in step (1) is 8-12cm, and the cuttage depth of the scion wood is 1-3 cm.
3. The cuttage propagation seedling raising method for pinus khasys according to claim 1, wherein the treatment solution in step (1) is 4000 times biological agent solution and ABT with concentration of 1000mg/L1。
4. The cuttage propagation seedling raising method for pinus khasys according to claim 1, characterized in that the humidity in the greenhouse is maintained to be more than 90% within 1 month after the cutting of the scion wood in the step (2), and the humidity in the greenhouse is maintained to be more than 80% after 1 month.
5. The cuttage propagation seedling raising method for pinus khasys according to claim 1, wherein the bactericide in step (2) is 800 times liquid of carbendazim or 1000 times liquid of methyl mop water; the fertilizer is urea, and the mass concentration of the urea is 1%.
6. The method for raising seedlings of pinus khasys by cutting propagation according to claim 1, wherein the moldy ears are found to be treated in time during the management of cutting seedlings, and 1500-fold liquid of hymexazol is selected for control.
7. The cutting propagation seedling raising method of pinus khasys according to claim 1, wherein the slow release fertilizer is applied once after full light seedling hardening in step (3), the application amount of the slow release fertilizer is 3 g/plant, and the application method of the slow release fertilizer is as follows: and guiding a small hole with the depth of about 2cm by using bamboo chopsticks in the nutrition bag, and then applying slow release fertilizer, wherein the slow release fertilizer is N: p2O5：K2The mass ratio of O is 16:9: 12.
8. The cutting propagation seedling raising method of pinus khasys according to claim 1, wherein in step (4), the land is completely cleaned before forestation, then the hole-shaped land is used for soil preparation, planting holes are dug along the contour line, the specification is 40cm x 30cm, surface soil and subsoil are respectively placed at one side of each hole during hole digging, the surface soil is returned to the pond, then the subsoil is returned, a small hole of 20cm x 20cm is dug in the middle after soil returning, seedlings are placed, the surrounding soil is treaded tightly, then the soil is covered into steamed bread shape, and the pine needle base is prevented from being buried into the soil.
9. The cuttage propagation seedling raising method for pinus khasys according to claim 1, wherein in the step (4), the compound fertilizer is N: p2O5：K2The mass ratio of O is 15:15: 15.
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| CN102113445A (en) * | 2010-09-10 | 2011-07-06 | 普洱市林业科学研究所 | Method for raising seedlings of Pinus kesiya var.langbianensis by efficient twig cuttage propagation |
| CN104106360A (en) * | 2014-07-06 | 2014-10-22 | 普洱市林业科学研究所 | Method for shoot cuttage rejuvenation of superior adult trees of pinus khasys |
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| CN102113445A (en) * | 2010-09-10 | 2011-07-06 | 普洱市林业科学研究所 | Method for raising seedlings of Pinus kesiya var.langbianensis by efficient twig cuttage propagation |
| CN104106360A (en) * | 2014-07-06 | 2014-10-22 | 普洱市林业科学研究所 | Method for shoot cuttage rejuvenation of superior adult trees of pinus khasys |
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