CN1132845C - 获得具有正确键合的胱氨酸键的胰岛素前体的改进的方法 - Google Patents

获得具有正确键合的胱氨酸键的胰岛素前体的改进的方法 Download PDF

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Publication number
CN1132845C
CN1132845C CN98117909A CN98117909A CN1132845C CN 1132845 C CN1132845 C CN 1132845C CN 98117909 A CN98117909 A CN 98117909A CN 98117909 A CN98117909 A CN 98117909A CN 1132845 C CN1132845 C CN 1132845C
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China
Prior art keywords
amino
insulin
acid residue
peptide
arg
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Expired - Lifetime
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CN98117909A
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English (en)
Chinese (zh)
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CN1209437A (zh
Inventor
F-J·鲁伯洛德
R·凯勒
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sanofi Aventis Deutschland GmbH
Original Assignee
Hoechst Marion Roussel Deutschland GmbH
Aventis Pharma Deutschland GmbH
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Publication of CN1209437A publication Critical patent/CN1209437A/zh
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • A61P5/48—Drugs for disorders of the endocrine system of the pancreatic hormones
    • A61P5/50—Drugs for disorders of the endocrine system of the pancreatic hormones for increasing or potentiating the activity of insulin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/02—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Diabetes (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Endocrinology (AREA)
  • Analytical Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Hematology (AREA)
  • Emergency Medicine (AREA)
  • Obesity (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
CN98117909A 1997-08-18 1998-08-17 获得具有正确键合的胱氨酸键的胰岛素前体的改进的方法 Expired - Lifetime CN1132845C (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19735711A DE19735711C2 (de) 1997-08-18 1997-08-18 Verfahren zur Herstellung eines Vorläufers von Insulin oder Insulinderivaten mit korrekt verbundenen Cystinbrücken
DE19735711.3 1997-08-18

Related Child Applications (2)

Application Number Title Priority Date Filing Date
CN031523307A Division CN1483831B (zh) 1997-08-18 1998-08-17 获得具有正确键合的胱氨酸键的胰岛素或胰岛素衍生物的方法
CN2006101000625A Division CN101186940B (zh) 1997-08-18 1998-08-17 获得具有正确键合的胱氨酸键的胰岛素或胰岛素衍生物的方法

Publications (2)

Publication Number Publication Date
CN1209437A CN1209437A (zh) 1999-03-03
CN1132845C true CN1132845C (zh) 2003-12-31

Family

ID=7839275

Family Applications (3)

Application Number Title Priority Date Filing Date
CN98117909A Expired - Lifetime CN1132845C (zh) 1997-08-18 1998-08-17 获得具有正确键合的胱氨酸键的胰岛素前体的改进的方法
CN2006101000625A Expired - Lifetime CN101186940B (zh) 1997-08-18 1998-08-17 获得具有正确键合的胱氨酸键的胰岛素或胰岛素衍生物的方法
CN031523307A Expired - Lifetime CN1483831B (zh) 1997-08-18 1998-08-17 获得具有正确键合的胱氨酸键的胰岛素或胰岛素衍生物的方法

Family Applications After (2)

Application Number Title Priority Date Filing Date
CN2006101000625A Expired - Lifetime CN101186940B (zh) 1997-08-18 1998-08-17 获得具有正确键合的胱氨酸键的胰岛素或胰岛素衍生物的方法
CN031523307A Expired - Lifetime CN1483831B (zh) 1997-08-18 1998-08-17 获得具有正确键合的胱氨酸键的胰岛素或胰岛素衍生物的方法

Country Status (20)

Country Link
US (3) US5986048A (https=)
EP (2) EP0906918B1 (https=)
JP (1) JP4291900B2 (https=)
KR (2) KR100537842B1 (https=)
CN (3) CN1132845C (https=)
AR (1) AR016821A1 (https=)
AT (2) ATE217636T1 (https=)
AU (1) AU744824B2 (https=)
BR (2) BRPI9816233B8 (https=)
CA (1) CA2245151C (https=)
CZ (1) CZ295679B6 (https=)
DE (3) DE19735711C2 (https=)
DK (2) DK0980874T3 (https=)
ES (2) ES2177178T3 (https=)
HU (2) HU228161B1 (https=)
ID (1) ID20717A (https=)
PL (1) PL191901B1 (https=)
PT (2) PT906918E (https=)
RU (2) RU2205836C2 (https=)
TR (1) TR199801587A3 (https=)

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DE19735711C2 (de) 1997-08-18 2001-04-26 Aventis Pharma Gmbh Verfahren zur Herstellung eines Vorläufers von Insulin oder Insulinderivaten mit korrekt verbundenen Cystinbrücken
DE19930676B4 (de) 1999-07-02 2006-01-19 Sanofi-Aventis Deutschland Gmbh Verfahren zur Stabilisierung von Insulin, Insulinderivaten und/oder deren Vorläufer in komplexen Mischungen bei deren Lagerung in wäßrigen Lösungsmitteln
WO2001046453A1 (en) * 1999-12-22 2001-06-28 Novo Nordisk A/S Method for extractive refolding of scrambled single-chain polypeptides
US20030212248A1 (en) * 2000-07-12 2003-11-13 Furman Thomas Charles Process to increase protein stability
DE10235168A1 (de) * 2002-08-01 2004-02-12 Aventis Pharma Deutschland Gmbh Verfahren zur Reinigung von Preproinsulin
DE102004015965A1 (de) * 2004-04-01 2005-10-20 Aventis Pharma Gmbh Verfahren zur Gewinnung von Insulinen durch verbesserte Luftbegasung der Faltung
NZ568809A (en) 2005-12-22 2011-08-26 Genentech Inc Recovering and purification of VEGF proteins from prokaryotic cells using polyanionic agents
US20080125580A1 (en) 2006-07-14 2008-05-29 Genentech, Inc. Refolding of Recombinant Proteins
JP5244175B2 (ja) * 2007-07-03 2013-07-24 アムジエン・インコーポレーテツド 封入体乾燥重量を用いたタンパク質の測定
RU2451750C2 (ru) * 2007-09-24 2012-05-27 Общество С Ограниченной Ответственностью "Герофарм" Способ получения рекомбинантного с-пептида проинсулина человека
CN102007143B (zh) 2008-01-09 2015-08-26 塞诺菲-安万特德国有限公司 具有超延迟时效特征的新型胰岛素衍生物
WO2009133529A2 (en) * 2008-04-30 2009-11-05 Wockhardt Research Centre Processes for refolding of insulin
CN102256618A (zh) 2008-10-17 2011-11-23 赛诺菲-安万特德国有限公司 胰岛素和glp-1激动剂的组合
TR201809460T4 (tr) 2009-11-13 2018-07-23 Sanofi Aventis Deutschland Bir GLP- 1-agonisti, bir insülin ve metiyonin içeren farmasötik bileşim.
NZ599847A (en) 2009-11-13 2013-09-27 Sanofi Aventis Deutschland Pharmaceutical composition comprising a glp-1 agonist and methionine
WO2012028172A1 (en) 2010-08-30 2012-03-08 Sanofi-Aventis Deutschland Gmbh Use of ave0010 for the manufacture of a medicament for the treatment of diabetes mellitus type 2
US9821032B2 (en) 2011-05-13 2017-11-21 Sanofi-Aventis Deutschland Gmbh Pharmaceutical combination for improving glycemic control as add-on therapy to basal insulin
TWI559929B (en) 2011-09-01 2016-12-01 Sanofi Aventis Deutschland Pharmaceutical composition for use in the treatment of a neurodegenerative disease
US12157766B2 (en) * 2012-08-05 2024-12-03 Absci Corporation Cytoplasmic expression system
EP2931301B2 (en) 2012-12-17 2021-09-15 Merck Sharp & Dohme Corp. Process for purifying insulin and analogues thereof
TWI708782B (zh) * 2013-02-26 2020-11-01 南韓商韓美藥品股份有限公司 新穎胰島素類似物及其用途
CN103694339B (zh) * 2013-12-04 2017-10-13 珠海联邦制药股份有限公司 一种甘精胰岛素前体的复性方法
EP3116531B1 (en) 2014-03-14 2021-10-20 Merck Sharp & Dohme Corp. Purifying insulin using cation exchange and reverse phase chromatography in the presence of an organic modifier and elevated temperature
PE20171622A1 (es) 2014-12-12 2017-11-02 Sanofi Aventis Deutschland Formulacion de relacion fija de insulina glargina/lixisenatida
TWI748945B (zh) 2015-03-13 2021-12-11 德商賽諾菲阿凡提斯德意志有限公司 第２型糖尿病病患治療
TW201705975A (zh) 2015-03-18 2017-02-16 賽諾菲阿凡提斯德意志有限公司 第２型糖尿病病患之治療
CN104911203A (zh) * 2015-06-30 2015-09-16 成都易胜科生物科技有限公司 一种重组人胰岛素的工业制备方法
US10745457B2 (en) 2015-09-02 2020-08-18 Merck Sharp & Dohme Corp. Process for obtaining insulin with correctly formed disulfide bonds
US10689430B2 (en) 2016-05-25 2020-06-23 Merck Sharp & Dohme Corp. Insulin receptor partial agonists
WO2018018613A1 (zh) 2016-07-29 2018-02-01 广东东阳光药业有限公司 一种提高抗体纯度的细胞培养基和培养方法
CN113773391B (zh) * 2020-06-09 2023-10-20 宁波鲲鹏生物科技有限公司 一种门冬胰岛素的制备方法
CN113773400B (zh) * 2020-06-09 2023-08-18 宁波鲲鹏生物科技有限公司 一种门冬胰岛素衍生物及其应用
CN113773396A (zh) * 2020-06-10 2021-12-10 宁波鲲鹏生物科技有限公司 一种地特胰岛素衍生物及其应用
JP7749397B2 (ja) * 2021-09-30 2025-10-06 キヤノン株式会社 超音波ファントム及び超音波ファントムの製造方法

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NZ199391A (en) * 1981-01-02 1985-12-13 Genentech Inc Chimeric polypeptides comprising a proinsulin sequence,and preparation by recombinant dna technique;production of human insulin
DE3501641A1 (de) * 1985-01-19 1986-07-24 Hoechst Ag, 6230 Frankfurt Verfahren zur gewinnung von insulin-vorlaeufern aus reaktionsgemischen, die bei der faltung von insulin-vorlaeufern aus den entsprechenden s-sulfonaten anfallen
IL95495A (en) * 1989-08-29 1996-10-16 Hoechst Ag Fusion proteins their preparation and use
RU2045535C1 (ru) * 1991-06-18 1995-10-10 Институт биоорганической химии им.М.М.Шемякина и Ю.А.Овчинникова РАН Способ получения проинсулина человека
DK0600372T3 (da) * 1992-12-02 1997-08-11 Hoechst Ag Fremgangsmåde til fremstilling af proinsulin med korrekt forbundne cystinbroer.
DE4405179A1 (de) * 1994-02-18 1995-08-24 Hoechst Ag Verfahren zur Gewinnung von Insulin mit korrekt verbundenen Cystinbrücken
RU2081122C1 (ru) * 1994-06-16 1997-06-10 Институт биоорганической химии им.М.М.Шемякина и Ю.А.Овчинникова РАН Способ выделения и очистки инсулина или его биотехнологических предшественников
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Also Published As

Publication number Publication date
RU2205836C2 (ru) 2003-06-10
HU228161B1 (en) 2013-01-28
EP0906918A2 (de) 1999-04-07
ES2176870T3 (es) 2002-12-01
HK1061870A1 (en) 2004-10-08
DE19735711A1 (de) 1999-02-25
CZ295679B6 (cs) 2005-09-14
DK0906918T3 (da) 2002-09-09
US6727346B2 (en) 2004-04-27
ID20717A (id) 1999-02-18
KR19990023651A (ko) 1999-03-25
HU228203B1 (en) 2013-01-28
JP4291900B2 (ja) 2009-07-08
US20020156234A1 (en) 2002-10-24
US5986048A (en) 1999-11-16
RU2302882C2 (ru) 2007-07-20
US6380355B1 (en) 2002-04-30
ATE217012T1 (de) 2002-05-15
EP0906918B1 (de) 2002-05-15
CA2245151C (en) 2009-10-06
DK0980874T3 (da) 2002-08-19
CN1209437A (zh) 1999-03-03
KR100537842B1 (ko) 2006-03-23
CN1483831A (zh) 2004-03-24
KR100574580B1 (ko) 2006-04-28
HU0600287D0 (en) 2006-05-29
CN101186940A (zh) 2008-05-28
CA2245151A1 (en) 1999-02-18
HUP9801886A2 (hu) 1999-01-28
DE59804121D1 (de) 2002-06-20
EP0906918A3 (de) 1999-08-11
BRPI9816233B8 (pt) 2021-05-25
EP0980874B1 (de) 2002-05-02
AR016821A1 (es) 2001-08-01
PL328108A1 (en) 1999-03-01
JPH11130798A (ja) 1999-05-18
KR20050067371A (ko) 2005-07-01
ES2177178T3 (es) 2002-12-01
HK1018464A1 (en) 1999-12-24
HK1119449A1 (en) 2009-03-06
PT906918E (pt) 2002-10-31
HU9801886D0 (en) 1998-10-28
BRPI9816233B1 (pt) 2016-09-27
DE59803985D1 (de) 2002-06-06
TR199801587A2 (xx) 1999-03-22
TR199801587A3 (tr) 1999-03-22
CN101186940B (zh) 2012-02-08
AU744824B2 (en) 2002-03-07
PT980874E (pt) 2002-10-31
ATE217636T1 (de) 2002-06-15
HUP9801886A3 (en) 1999-06-28
CN1483831B (zh) 2012-06-13
BR9803755A (pt) 2000-03-28
AU8076398A (en) 1999-02-25
EP0980874A1 (de) 2000-02-23
PL191901B1 (pl) 2006-07-31
CZ259898A3 (cs) 1999-03-17
DE19735711C2 (de) 2001-04-26

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C53 Correction of patent of invention or patent application
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Applicant after: Awentis Medicines Deutschland GmbH

Applicant before: German Beh Chester Marie

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Free format text: CORRECT: APPLICANT; FROM: HOECHST MARION GERMANY; MOSHIER LTD. TO: AVENTIS PHARMACY (GERMANY)INTERNATIONAL CO., LTD.

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Granted publication date: 20031231