CN112316123A - Traditional Chinese medicine composition for resisting helicobacter pylori infection and preparation method and application thereof - Google Patents
Traditional Chinese medicine composition for resisting helicobacter pylori infection and preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine composition for resisting helicobacter pylori infection, which comprises the following raw materials: the traditional Chinese medicine composition is formed by combining probiotics, lysozyme and Chinese medicines, and can effectively inhibit helicobacter pylori until helicobacter pylori in a human body is eliminated, reduce the occurrence of gastric cancer and have no harm to the human body, and the composition can be applied to toothpaste and combined with other matrixes, so that the helicobacter pylori loses the living environment in the oral cavity, and is killed by natural bactericidal medicines, healthy and safe, the disease is prevented from entering the oral cavity, the environment in the oral cavity is not damaged, and the occurrence of stomach diseases is fundamentally prevented.
Description
Technical Field
The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition for resisting helicobacter pylori infection and a preparation method and application thereof.
Background
Helicobacter pylori (h. pylori) is a spiroid, microaerophilic, gram-negative bacterium that is very demanding on growth conditions, and is currently the only microorganism species known to be able to survive in the human stomach. Helicobacter pylori mainly enters the stomach of a human body through an oral cavity and is planted on the surface of epithelial cells, an organism after planting is difficult to remove spontaneously, the helicobacter pylori can almost cause active inflammation of gastric mucosa, a series of common diseases such as chronic gastritis, chronic atrophic gastritis, peptic ulcer and gastric cancer can also occur on the basis of the active inflammation, and the health of people is seriously harmed.
For the treatment of helicobacter pylori, the existing medical method adopts a large dose of a plurality of antibiotics to kill the helicobacter pylori at the same time, but the method can kill a large amount of beneficial bacteria while killing the helicobacter pylori, so that the disease is recurrent, moreover, the drug resistance of the helicobacter pylori is increased by the administration of the large dose of the plurality of antibiotics, the drug resistance generated by the bacteria reduces or loses the treatment effect of the originally effective antibiotics, and the treatment difficulty and the medical cost of the disease of a patient are increased.
Therefore, how to provide an effective and safe traditional Chinese medicine composition for resisting helicobacter pylori infection is a problem to be solved urgently by related people in the field.
Disclosure of Invention
The composition can be applied to toothpaste, is combined with other substrates of the toothpaste together, enables the helicobacter pylori to lose the living environment in the oral cavity, kills the helicobacter pylori through natural bactericidal medicines, is healthy and safe, prevents the disease from entering the oral cavity, does not damage the environment in the oral cavity, and fundamentally prevents the occurrence of stomach diseases.
In order to achieve the purpose, the invention adopts the following technical scheme:
a traditional Chinese medicine composition for resisting helicobacter pylori infection comprises the following raw materials: lysozyme, probiotics, clove leaves, wrinkled gianthyssop, eupatorium, evodia and coptis.
The invention provides a traditional Chinese medicine composition, which solves the problem of drug resistance caused by using antibiotics in the prior art. On one hand, the invention adopts several traditional Chinese medicines of clove leaves, wrinkled gianthyssop herb, eupatorium, evodia rutaecarpa and coptis root for compatibility, has the action characteristics of multiple components, multiple ways and multiple targets, and has low drug resistance incidence rate of traditional Chinese medicine therapy, less adverse reaction and good patient drug compliance; on the other hand, the lysozyme and the probiotics are added into the traditional Chinese medicine composition, and the traditional Chinese medicine composition and the probiotics are cooperated, so that the beneficial bacteria in the stomach can be proliferated, the harmful bacteria helicobacter pylori in the stomach can be inhibited, the effect of regulating the flora in the stomach is achieved, the proliferation of immune cells in the stomach can be promoted, and the health care effects of relaxing bowel and enhancing immunity are achieved.
Preferably, the method comprises the following raw materials: according to the weight portion, 15-30 portions of lysozyme, 10-20 portions of probiotics, 8-15 portions of clove leaves, 5-10 portions of wrinkled gianthyssop herb, 5-15 portions of fortune eupatorium herb, 8-16 portions of evodia rutaecarpa and 7-15 portions of coptis chinensis.
Preferably, the method comprises the following raw materials: according to the weight portion, 20 portions of lysozyme, 15 portions of probiotics, 9 portions of clove leaves, 8 portions of wrinkled gianthyssop herb, 10 portions of fortune eupatorium herb, 10 portions of evodia fruit and 11 portions of coptis chinensis.
The formula adopted by the invention exerts the effects of all substances to the maximum effect, and the effective components are matched with each other to achieve the highest effect.
Preferably, the probiotics comprise lactobacillus reuteri powder, lactobacillus plantarum powder, lactobacillus salivarius powder and lactobacillus rhamnosus powder in a mass ratio of 1:3-5:2-5: 4-6.
The probiotics can strengthen the barrier effect of mucosa, reduce inflammatory reaction, improve clinical symptoms and compliance of patients in the treatment process, directly or indirectly improve the eradication rate of helicobacter pylori, and have important significance for preventing and treating the helicobacter pylori and related diseases.
The preparation method of the traditional Chinese medicine composition for resisting helicobacter pylori infection specifically comprises the following steps:
(1) weighing raw materials including lysozyme, probiotics, clove leaves, agastache rugosus, eupatorium, evodia rutaecarpa and coptis for later use;
(2) uniformly mixing the clove leaves, the agastache rugosus, the eupatorium fortunei, the evodia rutaecarpa and the coptis chinensis, adding water for soaking for 0.5-1h, then boiling, refluxing and extracting for 1-2h, filtering to obtain a filtrate, concentrating, finally adding absolute ethyl alcohol, uniformly stirring, standing for 24-48h, sucking a supernatant, and concentrating to obtain an extracting solution;
(3) and after the extracting solution is subjected to spray drying, uniformly mixing the extracting solution with the lysozyme and the probiotics to obtain the traditional Chinese medicine composition for resisting helicobacter pylori infection.
The preparation method is simple, and ensures the activity of the probiotics and the lysozyme on the basis of ensuring that the effective components are fully extracted, thereby exerting the maximum effect.
Preferably, the reflux extraction in step (2) is performed for 3 times, wherein the addition amount of water is in turn the mass of the raw materials: 15-20 times, 7-10 times and 7-10 times; the addition amount of the absolute ethyl alcohol is 3-5 times of the mass of the mixed raw materials.
Preferably, the filtrate in step (2) is concentrated to a relative density of 1.00-1.20.
Preferably, the supernatant in step (2) is concentrated to a relative density of 0.90-1.10.
Preferably, the conditions for the concentration in step (2) are: steam pressure is 0.08-0.1MPa, vacuum degree is-0.1-0.02 MPa, and concentration temperature is 55-95 ℃.
The invention also claims application of the traditional Chinese medicine composition or the traditional Chinese medicine composition prepared by the preparation method in toothpaste.
The composition can be applied to toothpaste and combined with other substrates of the toothpaste, so that helicobacter pylori loses the living environment in the oral cavity, and the helicobacter pylori is killed by natural bactericidal drugs, thereby being healthy and safe, preventing diseases from entering the oral cavity, not damaging the environment in the oral cavity, and fundamentally preventing the occurrence of stomach diseases.
Preferably, the traditional Chinese medicine composition accounts for 0.5-10% of the total mass of the toothpaste, and the balance is toothpaste matrix.
According to the technical scheme, compared with the prior art, the invention has the following beneficial effects: the invention provides a Chinese medicinal composition for resisting helicobacter pylori infection and its preparing process, wherein the composition is prepared from probiotics, lysozyme and Chinese medicinal herbs, the three are combined synergistically, can effectively inhibit helicobacter pylori, remove helicobacter pylori in human body, reduce gastric cancer, and has no harm to human body.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A preparation method of a traditional Chinese medicine composition for resisting helicobacter pylori infection specifically comprises the following steps:
(1) weighing raw materials: according to the weight parts, 15 parts of lysozyme, 10 parts of probiotics, 8 parts of clove leaves, 5 parts of agastache rugosus, 5 parts of eupatorium, 8 parts of fructus evodiae and 7 parts of coptis chinensis for later use; wherein the probiotics comprise lactobacillus reuteri powder, lactobacillus plantarum powder, lactobacillus salivarius powder and lactobacillus rhamnosus powder in a mass ratio of 1:3:2: 4;
(2) pulverizing folium Caryophylli, herba Agastaches, herba Eupatorii, fructus evodiae and Coptidis rhizoma, mixing, adding 15 times of water, soaking for 0.5 hr, heating to boil, and reflux extracting for 1 hr; filtering, wherein the filtrate is a 100-mesh filter cloth, adding water 7 times the mass of the Chinese medicinal materials into the residue, boiling, and extracting under reflux for 1 hr; after filtering, the filtrate is reserved, the used filtering cloth is 100-mesh and 150-mesh filtering cloth, water with the mass being 7 times that of the traditional Chinese medicine raw material is added into the filter residue, boiling and reflux extraction is carried out for 1 hour, and after filtering, the filtrate is reserved, and the used filtering cloth is 100-mesh and 150-mesh filtering cloth; reflux-extracting for three times, mixing the three filtrates, and concentrating in a concentrator to relative density of 1.00(20 deg.C); then adding anhydrous ethanol with the mass 3 times of that of the traditional Chinese medicine raw material, standing for 24h, sucking supernatant, and concentrating the supernatant in a concentrator to the relative density of 0.9(20 ℃), thus obtaining an extracting solution; the steam pressure of the concentrator is 0.08MPa, the vacuum degree is-0.1 MPa, and the concentration temperature is 55 ℃;
(3) spray drying the extractive solution, spray drying at inlet temperature of 190 deg.C and outlet temperature of 95 deg.C, and mixing with lysozyme and probiotic bacteria to obtain the Chinese medicinal composition for resisting helicobacter pylori infection.
Example 2
A preparation method of a traditional Chinese medicine composition for resisting helicobacter pylori infection specifically comprises the following steps:
(1) weighing raw materials: according to the weight parts, 30 parts of lysozyme, 20 parts of probiotics, 15 parts of clove leaves, 10 parts of wrinkled gianthyssop herb, 15 parts of fortune eupatorium herb, 16 parts of evodia rutaecarpa and 15 parts of coptis chinensis for later use; wherein the probiotics comprise lactobacillus reuteri powder, lactobacillus plantarum powder, lactobacillus salivarius powder and lactobacillus rhamnosus powder in a mass ratio of 1:5:5: 6;
(2) pulverizing folium Caryophylli, herba Agastaches, herba Eupatorii, fructus evodiae and Coptidis rhizoma, mixing, adding 20 times of water, soaking for 1 hr, heating to boil, and reflux extracting for 2 hr; filtering, wherein the filtrate is a 150-mesh filter cloth, adding water 10 times the weight of the Chinese medicinal materials into the residue, boiling, and extracting under reflux for 2 hr; after filtering, the filtrate is reserved, the filtering is 100-mesh filter cloth with 150 meshes, water with the mass being 10 times that of the traditional Chinese medicine raw material is added into the filter residue, boiling and reflux extraction is carried out for 2 hours, and after filtering, the filtrate is reserved, and the filtering is 100-mesh filter cloth with 150 meshes; reflux-extracting for three times, mixing the three filtrates, and concentrating in a concentrator to relative density of 1.20(20 deg.C); then adding anhydrous ethanol 5 times the mass of the traditional Chinese medicine raw materials, standing for 48h, sucking supernatant, and concentrating the supernatant in a concentrator to relative density of 1.10(20 ℃), thus obtaining an extracting solution; the steam pressure of the concentrator is 0.1MPa, the vacuum degree is-0.2 MPa, and the concentration temperature is 95 ℃;
(3) spray drying the extractive solution at inlet temperature of 200 deg.C and outlet temperature of 100 deg.C, and mixing with lysozyme and probiotic bacteria to obtain the Chinese medicinal composition for resisting helicobacter pylori infection.
Example 3
A preparation method of a traditional Chinese medicine composition for resisting helicobacter pylori infection specifically comprises the following steps:
(1) weighing raw materials: according to the weight parts, 20 parts of lysozyme, 15 parts of probiotics, 9 parts of clove leaves, 8 parts of agastache rugosus, 10 parts of eupatorium, 10 parts of evodia rutaecarpa and 11 parts of coptis chinensis for later use; wherein the probiotics comprise lactobacillus reuteri powder, lactobacillus plantarum powder, lactobacillus salivarius powder and lactobacillus rhamnosus powder in a mass ratio of 1:4:4: 5;
(2) pulverizing folium Caryophylli, herba Agastaches, herba Eupatorii, fructus evodiae and Coptidis rhizoma, mixing, adding 20 times of water, soaking for 1 hr, heating to boil, and reflux extracting for 2 hr; after filtering, the filtrate is reserved, the used filtering is 100-mesh filter cloth with 150 meshes, water with the mass of 10 times of that of the traditional Chinese medicine raw material is added into the filter residue, and the mixture is boiled and extracted for 2 hours in a reflux manner; after filtering, the filtrate is reserved, the used filtering is 100-mesh filtering cloth with 150 meshes, water with the mass being 10 times that of the traditional Chinese medicine raw material is added into the filter residue, boiling and reflux extraction is carried out for 2 hours, and after filtering, the filtrate is reserved, and the used filtering is 100-mesh filtering cloth; reflux-extracting for three times, mixing the three filtrates, and concentrating in a concentrator to relative density of 1.20(20 deg.C); then adding anhydrous ethanol 5 times the mass of the traditional Chinese medicine raw materials, standing for 48h, sucking supernatant, and concentrating the supernatant in a concentrator to relative density of 1.10(20 ℃), thus obtaining an extracting solution; the steam pressure of the concentrator is 0.1MPa, the vacuum degree is-0.2 MPa, and the concentration temperature is 95 ℃;
(3) spray drying the extractive solution at inlet temperature of 200 deg.C and outlet temperature of 100 deg.C, and mixing with lysozyme and probiotic bacteria to obtain the Chinese medicinal composition for resisting helicobacter pylori infection.
Comparative example 1
No lysogen was added to the raw materials, and the remaining raw materials and the operation steps were completely the same as in example 3.
Comparative example 2
No probiotics were added to the raw materials and the remaining raw materials and operating procedures were exactly the same as in example 3.
Comparative example 3
The raw materials only adopt 20 parts of lysozyme and 15 parts of probiotics, and the operation steps are completely the same as those of the example 3.
Comparative example 4
The raw materials are not added with probiotics and lysozyme, and the rest raw materials and the operation steps are completely the same as the example 3.
Application example 1
500 patients with gastric ulcer, which were clinically recognized to have gastric ulcer caused by helicobacter pylori, were selected as test drug volunteers. Wherein each gastric ulcer patient13C urease breath test positive and has at least one of the following symptoms: abdominal distension, acid regurgitation, belching, nausea, heartburn, chest pain, hematemesis, anorexia, dark stool, epigastric fullness after eating, epigastric discomfort after eating, epigastric pain after eating, and small amount of upper gastrointestinal hemorrhage.
Divided into 5 groups, each group using the drugs prepared in example 3 and comparative examples 1 to 4 of the present invention, respectively, and each group was infused with warm water before supper for 20 to 30g of the drugs prepared in example 3 and comparative examples 1 to 4 for 20 days, and then the symptoms of each patient were recorded,13c urease breath test helicobacter pylori, the results are shown in Table 1.
TABLE 1 Experimental results of example 3 and comparative examples 1-3
| Negative for helicobacter pylori | Symptom relief | Invalidation | |
| Example 3 | 90 | 10 | 0 |
| Comparative example 1 | 29 | 71 | 0 |
| Comparative example 2 | 36 | 64 | 0 |
| Comparative example 3 | 0 | 70 | 30 |
| Comparative example 4 | 12 | 88 | 0 |
As can be seen from the data in Table 1, the technical effect of the traditional Chinese medicine composition is obviously superior to that of comparative examples 1-4, and the traditional Chinese medicine composition can effectively inhibit helicobacter pylori until helicobacter pylori in a human body is eliminated, and has a synergistic effect.
Application example 2
The traditional Chinese medicine composition of the embodiment 3 and the comparative examples 1 to 4 is added into toothpaste, and the traditional Chinese medicine composition and a toothpaste matrix are uniformly mixed, wherein the traditional Chinese medicine composition accounts for 8 percent of the total mass of the toothpaste, and the toothpaste matrix is humectant, thickener, adhesive, foaming agent, friction agent, sweetener, aromatic agent and the like added in the traditional subspecies.
Mixing helicobacter pylori cultured at 37 deg.C for 24 hr with normal saline to reach mixed bacteria content of 105cfu/mL; uniformly coating 0.1ml of bacterial liquid on a beef extract protein solid culture medium, respectively taking the toothpaste prepared in the embodiment 3 and the comparative examples 1-4 of the invention, wetting sterile filter paper sheets with the diameter of 10mm by sterile water, putting the filter paper sheets on a bacteria coating culture medium, culturing for 26h at 37 ℃, observing the diameter of a bacteriostatic circle, and judging the bacteriostatic ability of a sample; repeating the bacteriostasis experiment for 3 times, wherein the bacteriostasis result is the average value of the experiments of 3 times, and the result is shown in table 2;
TABLE 2 results of bacteriostatic experiments in example 3 and comparative examples 1 to 4
| Diameter of bacteriostatic circle (mm) | Growth of bacteria | |
| Example 3 | 35 | 0 |
| Comparative example 1 | 11 | ++ |
| Comparative example 2 | 16 | + |
| Comparative example 3 | 5 | +++ |
| Comparative example 4 | 8 | ++ |
Remarking: the "+" indicates how fast the bacteria multiply, and a larger "+" sign indicates a faster bacterial multiplication.
As can be seen from the data in Table 2, the oral cavity helicobacter pylori-resisting traditional Chinese medicine toothpaste disclosed by the invention has an obvious helicobacter pylori resisting effect, can completely kill helicobacter pylori, has an effect remarkably superior to that of comparative examples 1-4, and shows that the components are matched synergistically and have remarkable progress.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. A traditional Chinese medicine composition for resisting helicobacter pylori infection is characterized by comprising the following raw materials: lysozyme, probiotics, clove leaves, wrinkled gianthyssop, eupatorium, evodia and coptis.
2. The traditional Chinese medicine composition for resisting helicobacter pylori infection according to claim 1, which is characterized by comprising the following raw materials in parts by weight: 15-30 parts of lysozyme, 10-20 parts of probiotics, 8-15 parts of clove leaves, 5-10 parts of agastache rugosus, 5-15 parts of eupatorium, 8-16 parts of fructus evodiae and 7-15 parts of coptis chinensis.
3. The traditional Chinese medicine composition for resisting helicobacter pylori infection according to claim 1 or 2, wherein the probiotics comprise lactobacillus reuteri powder, lactobacillus plantarum powder, lactobacillus salivarius powder and lactobacillus rhamnosus powder in a mass ratio of 1:3-5:2-5: 4-6.
4. A method for preparing the Chinese medicinal composition for resisting helicobacter pylori infection according to any one of claims 1 to 3, which comprises the following steps:
(1) weighing raw materials including lysozyme, probiotics, clove leaves, agastache rugosus, eupatorium, evodia rutaecarpa and coptis for later use;
(2) uniformly mixing the clove leaves, the agastache rugosus, the eupatorium fortunei, the evodia rutaecarpa and the coptis chinensis, adding water for soaking for 0.5-1h, then boiling, refluxing and extracting for 1-2h, filtering to obtain a filtrate, concentrating, finally adding absolute ethyl alcohol, uniformly stirring, standing for 24-48h, sucking a supernatant, and concentrating to obtain an extracting solution;
(3) and after the extracting solution is subjected to spray drying, uniformly mixing the extracting solution with the lysozyme and the probiotics to obtain the traditional Chinese medicine composition for resisting helicobacter pylori infection.
5. The method for preparing a Chinese medicinal composition for resisting helicobacter pylori infection according to claim 4, wherein the reflux extraction in step (2) is performed 3 times, and the amounts of water added are, in order, based on the mass of the mixed raw materials: 15-20 times, 7-10 times and 7-10 times; the addition amount of the absolute ethyl alcohol is 3-5 times of the mass of the mixed raw materials.
6. The method for preparing a Chinese medicinal composition for resisting helicobacter pylori infection according to claim 4, wherein the filtrate in the step (2) is concentrated to a relative density of 1.00-1.20.
7. The method for preparing a Chinese medicinal composition for resisting helicobacter pylori infection according to claim 4, wherein the supernatant obtained in the step (2) is concentrated to a relative density of 0.90-1.10.
8. The method for preparing a Chinese medicinal composition for resisting helicobacter pylori infection according to claim 4, wherein the concentration conditions in the step (2) are as follows: steam pressure of 0.08-0.1MPa, vacuum degree of (-0.1) - (-0.02) MPa, and concentration temperature of 55-95 deg.C.
9. Use of the Chinese medicinal composition according to any one of claims 1 to 3 or the Chinese medicinal composition prepared by the preparation method according to any one of claims 4 to 8 in toothpaste.
10. The use of claim 9, wherein the traditional Chinese medicine composition accounts for 0.5-10% of the total mass of the toothpaste, and the balance is toothpaste base.
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