CN105726681A - Injection for treating dairy cattle endometritis and preparing method of injection - Google Patents

Abstract

The invention discloses an injection for treating dairy cattle endometritis and a preparing method of the injection. The injection is prepared from traditional Chinese medicine extract, wherein the traditional Chinese medicine extract is prepared from, by weight, 10-15 parts of herba leonuri, 15-20 parts of mangosteen peel, 7-12 parts of fresh enteromorpha prolifera, 6-9 parts of indigo naturalis, 8-10 parts of leaves or fruits of eucalyptus globulus, 4-7 parts of radix sophorae flavescentis, 8-10 parts of coptidis rhizoma, 3-5 parts of herba artemisiae scopariae, 6-9 parts of divaricate knotweed roots, 1-3 parts of hernandialeaf stephania roots, 4-8 parts of decumbent corydalis tubers, 5-9 parts of uniflower swisscentaury roots, 6-10 parts of herba rabdosiae rubescentis, 5-9 parts of bunge corydalis herb and 7-10 parts of honey-fried licorice roots. The injection is a pure traditional Chinese medicine preparation and can safely and effectively treat dairy cattle endometritis, it is avoided that the quality and effect are influenced due to the fact that diary cattle generate medicine resistance by large usage of antibiotic medicine, and the food safety of dairy cattle is effectively ensured.

Description

A kind of injection treating cow endometritis and preparation method thereof

Technical field

The present invention relates to Chinese veterinary drug preparation field, particularly to a kind of injection treating cow endometritis Liquid and preparation method thereof.

Background technology

Cow endometritis is the Reproduction Disorder that milch cow is most commonly seen, and sickness rate is high, and mortality rate is low, Often in chronic process, therefore usually can not cause the attention of people, cause bigger economic damage to milk cattle cultivating family Lose, be one of important diseases endangering milk cow production.Endometritis is mainly in cow puerperal, often in slowly Property pass through, although sickness rate is high but mortality rate is the lowest, severely impacts reproductive capacity and the productivity of milch cow Calving interval prolongation, milk yield can be made to reduce, mortality increases, and causes cattle during antibiotic therapy Milk is discarded, thus causes the biggest economic loss.The reason causing cow endometritis is varied, Except feeding and management is improper, environmental health condition is poor, clinical manipulation technology lack of standardization in addition to, also micro-with cause of disease The biological closely related pathogenic microorganism of infection mainly include antibacterial (staphylococcus aureus, escherichia coli, Streptococcus uberis, Klebsiella pneumonia etc.), virus, fungus, mycoplasma, parasite etc..At present, Antibiotic is the conventional medicine for the treatment of endometritis, but owing to antibiotic uses the most in a large number, on the one hand Causing Resistant strain to increase, the food-safety problem that on the other hand Residue of Antibiotics in Milk brings the most more comes More paid close attention to by people.After milch cow suffers from endometritis, due to heavy dose of, for a long time use various Antibiotic medicine, causes Ruzhong to there is residual antibiotic.When, after the breast that people eat containing antibiotic, drawing Play anaphylaxis such as erythra, discomfort, severe patient generation anaphylactic shock.More people are because of long-term drink Remain the Bovine Mastitis Milk of antibiotic, and in making body, antibacterial produces drug resistance, brings to the treatment of disease Bigger difficulty.Therefore safe prevention cow endometritis increasingly causes the attention of people.

Summary of the invention

It is an object of the invention to provide a kind of injection treating cow endometritis and preparation side thereof Method, this injection is pure Chinese medicinal preparation, can safely and effectively treat cow endometritis, it is to avoid a large amount of Use antibiotic medicine to make milch cow produce drug resistance and affect mass effect, effectively ensure that the food peace of milk Entirely.

To achieve these goals, the technical scheme that the present invention provides is as follows:

A kind of injection treating cow endometritis, comprises 20g mannitol in 1000ml injection, 60g sodium chloride, 50g Chinese medicine extract；Make described Chinese medicine extract raw material composition and parts by weight be: Herba Leonuri 10-15 part, mangosteen skin 15-20 part, Entermorpha fresh goods 7-12 part, Indigo Naturalis 6-9 part, Eucalyptus globulus Labill 8-10 Part, Radix Sophorae Flavescentis 4-7 part, Rhizoma Coptidis 8-10 part, Herba Artemisiae Scopariae 3-5 part, Radix polygoni divaricati 6-9 part, Radix Stephaniae hemandifoliae 1-3 part, Rhizoma Corydalis Decumbentis 4-8 part, Radix Rhapontici 5-9 part, Rabdosia rubescens 6-10 part, Herba Corydalis Bungeanae 5-9 part, Radix Glycyrrhizae Preparata 7-10 Part.

Further, make described Chinese medicine extract raw material composition and parts by weight be: Herba Leonuri 10 parts, Mangosteen skin 15 parts, Entermorpha fresh goods 7 parts, Indigo Naturalis 6 parts, Eucalyptus globulus Labill 8 parts, Radix Sophorae Flavescentis 4 parts, Rhizoma Coptidis 8 parts, Herba Artemisiae Scopariae 3 parts, Radix polygoni divaricati 6 parts, Radix Stephaniae hemandifoliae 1 part, Rhizoma Corydalis Decumbentis 4 parts, Radix Rhapontici 5 parts, Rabdosia rubescens 6 parts, Herba Corydalis Bungeanae 5 parts, Radix Glycyrrhizae Preparata 7 parts.

Further, make described Chinese medicine extract raw material composition and parts by weight be: Herba Leonuri 15 parts, Mangosteen skin 20 parts, Entermorpha fresh goods 12 parts, Indigo Naturalis 9 parts, Eucalyptus globulus Labill 10 parts, Radix Sophorae Flavescentis 7 parts, Rhizoma Coptidis 10 Part, Herba Artemisiae Scopariae 5 parts, Radix polygoni divaricati 9 parts, Radix Stephaniae hemandifoliae 3 parts, Rhizoma Corydalis Decumbentis 8 parts, Radix Rhapontici 9 parts, Rabdosia rubescens 10 parts, Herba Corydalis Bungeanae 9 parts, Radix Glycyrrhizae Preparata 10 parts.

Further, make described Chinese medicine extract raw material composition and parts by weight be: Herba Leonuri 12 Part, mangosteen skin 18 parts, Entermorpha fresh goods 10 parts, Indigo Naturalis 7 parts, Eucalyptus globulus Labill 9 parts, Radix Sophorae Flavescentis 5 parts, Rhizoma Coptidis 9 Part, Herba Artemisiae Scopariae 4 parts, Radix polygoni divaricati 7 parts, Radix Stephaniae hemandifoliae 2 parts, Rhizoma Corydalis Decumbentis 6 parts, Radix Rhapontici 7 parts, Rabdosia rubescens 8 Part, Herba Corydalis Bungeanae 7 parts, Radix Glycyrrhizae Preparata 8 parts.

The preparation method of the injection of above-mentioned treatment cow endometritis, comprises the following steps:

(1) Herba Leonuri, Indigo Naturalis, Eucalyptus globulus Labill, Radix Sophorae Flavescentis, Rhizoma Coptidis, Herba Artemisiae Scopariae, Radix polygoni divaricati, Radix Stephaniae hemandifoliae, summer Nothing, Radix Rhapontici, Rabdosia rubescens, Herba Corydalis Bungeanae, the broken 50 mesh mixed-powders that obtain of Radix Glycyrrhizae Preparata, the addition of gained mixed-powder The water soaking that gross mass multiple is 5 times 1 hour, slow fire boiling 1 hour, cross 500 mesh drainage screens after decoction Obtain filtrate, the again water soaking of addition mixed-powder gross mass multiple 5 times 0.5 hour, slow fire boiling 1 Cross 500 mesh drainage screens after hour and obtain filtrate, merge twice gained filtrate and be cooled to room temperature, standby；

(2) after mangosteen skin and Entermorpha fresh goods are cleaned, addition step (1) gained filtrate is squeezed the juice, and stands 1 little Filtered to get filtrate time after A；

(3) step (2) gained filtrate A cryoconcentration (less than 40 DEG C) to relative density be 1.35 ± The thick paste of 0.02 (30 DEG C), gained thick paste vacuum lyophilization, gained solid micronizing obtains 1000 mesh Powder is Chinese medicine extract；

(4) weigh the Chinese medicine extract of 50g step (3) gained, add 20g mannitol and 60g chlorine Change sodium, add the stirring of 200ml water for injection and make it dissolve, inject water to mixed solution further total Volume 1000ml, mixed solution is added homogenizer is carried out homogeneous, homogeneous temperature 15 ± 2 DEG C, the time Within 13 ± 2 minutes, obtaining homogeneous liquid, regulation pH value is 7.0, further obtains with 0.22 μm filter fine straining Filtrate；

(5) to step (4) gained filtrate ultraviolet sterilization 25 ± 5 minutes, gained liquid subpackage is become 20ml/ Injection and get final product.

Usage and dosage: ill domestic animal of growing up is injected 2 times on the 1st, the injection 20ml of the shot present invention, 6 Day is a course for the treatment of.

The raw materials used characteristic of Chinese medicine extract of the present invention is as follows: Herba Leonuri: bitter, pungent, is slightly cold, invigorates blood circulation Regulating menstruation, inducing diuresis to remove edema, heat-clearing and toxic substances removing, for menoxenia, dysmenorrhea amenorrhea, lochiorrhea, edema is urinated Few, sore swollen toxin；Mangosteen skin: slightly cool in nature, diminishing inflammation, removing pain, heat-clearing and toxic substances removing, dampness removing and anti-diarrhea effect, Mangosteen skin is rich in tannic acid, and tannic acid is to clinical common isolated strains such as staphylococcus aureus, epidermis Portugal Grape coccus, staphylococcus haemolyticus, enterococcus faecalis, enterococcus faecalis, escherichia coli, Klebsiella Pneumoniae, Pseudomonas aeruginosa, Acinetobacter bauamnnii etc. have antibacterial action and can affect biomembranous formation, Garcinia mangostana Skin is rich in procyanidin, and procyanidin is a kind of bioflavonoids having special molecular structure, is mesh Before in the world generally acknowledge the removing maximally effective Natural antioxidant of people's interior free yl, procyanidin can be strong Changing blood capillary, tremulous pulse and vein blood vessel, it has effect that detumescenceization is become silted up, the drag reduction of blood capillary Improving with permeability, make cell be easier to absorb nutrient and get rid of refuse, procyanidin can be with scavenger cell Water solublity and fat-soluble free radical in film, therefore, it is suppressed that discharge some enzyme and go to injure capillary wall Process, procyanidin suppression produces the enzyme that histamine needs, and prevents from generating histamine, thus dephlogistication； Entermorpha: be a kind of large-scale chlorella, be commonly called as Enteromorpha clathrata (Roth) Greville, green laver etc., for Chlorophyta Ulvales Ulvaceae Entermorpha The algae belonged to, common are Enteromorpha linza, Enteromorpha compressa(L.) Grev., Enteromorpha clathrata (Roth) Greville, intestinal Entermorpha etc., and Entermorpha is not only There is abundant nutritive value, and there is heat-clearing and toxic substances removing, anti-inflammation, cholesterol reducing, enhancing body Immunity, hard masses softening and resolving, swelling diuretic and effect of change disease.Record according to " occupying diet spectrum with breath ": waterside Tongue " gallbladder that disappears, the scrofula goiter that disappears, let out swollen, reduce phlegm, control being not acclimatized "；Compendium of Material Medica note has waterside Tongue " burn end nasal insufflation and stop epistaxis, soup is unrestrained smash deposited the back of the hand swell and ache "；Coastal spreading so far is controlled with ulva class decoction Acute and chronic gastroenteritis, Entermorpha is then relieved summer heat detoxification beverage by Guangdong.Entermorpha, rich in polysaccharide, accounts for Sargassum and does Weigh more than 50%, have widely biological activity and medicinal function (blood fat reducing, antitumor, antiviral, Antibacterial, regulation immunity, antioxidation, resisting fatigue and blood sugar lowering etc.), can be widely applied to medicine, health care Food and bread and cheese；Indigo Naturalis: salty, cold, heat clearing away, removing heat from blood, removing toxic substances, mass dissipating and swelling eliminating, control epidemic febrile disease heat Containing, macule, spit blood, spit blood, pediatric epilepsy scared, skin ulcer swells, and erysipelas, snake bite and insect sting, Indigo Naturalis alcohol immersion exists External to anthrax bacillus, streptococcus pneumoniae, shigella, vibrio cholera, staphylococcus aureus and Staphylococcus albus all has inhibitory action；Eucalyptus globulus Labill: the micro-hardship pungent, micro-of taste, property is put down.Dispelling wind is antipyretic, antibacterial Antiinflammatory, anticorrosion is antipruritic.For preventing influenza, epidemic cerebrospinal meningitis, control upper respiratory tract sense Dye, pharyngolaryngitis, bronchitis, pneumonia, Acute and chronic pyelonephritis, enteritis, dysentery, filaricide； Burn and scald, cellulitis, mastitis, furuncle and phyma, erysipelas, paddy-field dermatitis, skin eczema, foot are controlled in external Tinea, skin degerming；Radix Sophorae Flavescentis: bitter in the mouth, cold in nature, heat clearing and damp drying, antibacterial parasite killing, diuresis, for hematodiarrhoea, Have blood in stool, jaundice urine retention, leucorrhea with red and white discharge, swelling of the vulva pudendal pruritus, eczema, eczema, skin pruritus, scabies leprosy, External treatment trichomonal vaginitis；Rhizoma Coptidis: bitter in the mouth, cold, heat clearing and damp drying, eliminating fire and detoxication, anti-inflammation, use In damp and hot feeling of fullness, vomit acid regurgitation, dysentery, jaundice, unconsciousness due to high fever, hyperactivity of heart-fire, dysphoria and insomnia, blood Heat tells nosebleed, conjunctival congestion, toothache, quenches one's thirst, carbuncle furuncle, external treatment eczema, eczema, and auditory meatus is suppurated, and wine is yellow The kind clear part of the body cavity above the diaphragm housing the heart and lungs is burning hot, for conjunctival congestion, aphtha, prepared RHIZOMA COPTIDIS with rhizoma zingiberis recens juice clearing stomach stomach function regulating preventing or arresting vomiting, ties mutually for cold and heat, Damp and hot middle resistance, feeling of fullness vomiting, cornel Rhizoma Coptidis soothing liver-QI stomach function regulating preventing or arresting vomiting, for incoordination between the liver and stomach, vomit acid regurgitation, yellow Even and Radix Glycyrrhizae share can the effect of obvious its anti-Staphylococcus aureus of gain；Herba Artemisiae Scopariae: bitter, pungent, is slightly cold, Clearing away heat-damp and promoting diuresis, jaundice eliminating, cure mainly jaundice, dysuria, eczema pruritus etc., pharmaceutical research have function of gallbladder promoting, Liver function protecting, antipyretic, antiinflammatory, blood fat reducing, blood pressure lowering, coronary dilating etc. acts on, and in vitro tests proves, mattress Old decoct is to staphylococcus aureus, diphtheria corynebacterium, anthrax bacillus, Bacillus typhi, paratyphoid A bar Bacterium, bacillus pyocyaneus, escherichia coli, bacterium flexneri, dysentery bacterium, meningococcus, Bacillus subtilis etc. have inhibitory action in various degree；Radix polygoni divaricati: acid is sweet, temperature, cold relieving, the kidney warming, control Colic of cold type, scrotum is perspired, to staphylococcus aureus, Bacillus typhi, paratyphoid bacillus A, B-mode pair Bacillus typhi, escherichia coli O88B7, sonne bacillus, bacterium flexneri all have fungistatic effect； Radix Stephaniae hemandifoliae: bitter, cool, heat-clearing and toxic substances removing, eliminating stasis to stop pain, main flu, pharyngalgia, diarrhoea, dysentery, cellulitis swells Poison, stomachache, wind syndrome of head pain, rheumatic arthralgia, traumatic injury；Rhizoma Corydalis Decumbentis: warm in nature, bitter in the mouth, micro-pungent, live Blood, dredging collateral, promoting the circulation of QI to relieve pain, with controlling apoplectic hemiplegia, poliomyelitis sequela, sciatica, rheumatism Property arthritis, traumatic injury, lumbar muscle strain etc.；Radix Rhapontici: bitter in the mouth, cold in nature, enters stomach, large intestine channel, tool The effect having heat-clearing and toxic substances removing, eliminating carbuncle, stimulating milk secretion, relaxing muscles and tendons to promote blood circulation, swells and ache for acute mastitis, carbuncle carbuncle on the back, scrofula Scrofula sore, galactostasis, arthralgia chiefly caused by damp pathogen contracture, arthralgia, toxic-heat and blood stasis, bleeding hemorrhoids etc. are demonstrate,proved；Winter insults Grass: bitter in the mouth, sweet, cold nature, return lung, stomach, Liver Channel, there is heat-clearing and toxic substances removing, effect of promoting blood circulation and stopping pain, Cure mainly laryngopharynx swelling and pain, tonsillitis, headache due to common cold, tracheitis, chronic hepatitis, articular rheumatic pain, Serpentis Insect bite wounds etc. are demonstrate,proved, and can effectively suppress A type, beta hemolytic streptococcus, staphylococcus aureus etc., Thus improve human body resistance, reduce rapidly the leukocyte caused because of inflammation and increase；Herba Corydalis Bungeanae: bitter in the mouth, Cold in nature, GUIXIN, liver, large intestine channel, there is effect of heat-clearing and toxic substances removing, mass dissipating and swelling eliminating, cure mainly epidemic disease flu, Laryngopharynx swelling and pain, carbuncle carbuncle on the back, treat skin ulcer swell and ache wait demonstrate,prove；Radix Glycyrrhizae Preparata: containing glycyrrhizin, enoxolone, The multiple chemical composition such as Angelica Polysaccharide, has antiinflammatory and anti-allergy action, scalable immunity of organism merit Can, antitumor and analgesic effect, therefore the primary efficacy of Radix Glycyrrhizae Preparata is and middle emergency, lung moistening, removing toxic substances, Coordinating the actions of various ingredients in a prescription.

Chinese veterinarian's cow endometritis is called band disease, how because of female animal antibacterial in puerperal, the infection of virus, or Owing to pregnancy period feeding and management is not good at, conceived burden is excessive, and the factor such as excessive usage, childbirth causes QI and blood to hinder Stagnant or deficient, and then the positive deficiency of vital energy of body declines, pathogen is taken advantage of a weak point.Therefore, the injection of the present invention uses Anti-inflammation, heat-clearing and toxic substances removing, restore menstrual flow and invigorate blood circulation method treatment cow endometritis, as effective ingredient Chinese medicine extract raw material in, mangosteen skin, Indigo Naturalis, Rabdosia rubescens, Entermorpha, Eucalyptus globulus Labill, Radix Sophorae Flavescentis, Rhizoma Coptidis, Herba Artemisiae Scopariae, Radix polygoni divaricati can well play the effect of anti-inflammation, effectively suppression kill as staphylococcus, The pathogenic bacterium such as streptococcus, escherichia coli, corynebacterium, effectively treat cow endometritis, thus subtract The use of few antibiotic, wherein, in mangosteen skin rich in tannic acid to staphylococcus, staphylococcus epidermidis Etc. there being good antibacterial action, biomembranous formation can be affected；Mangosteen skin and Herba Leonuri, Indigo Naturalis, mattress Old, Radix Rhapontici, Rabdosia rubescens, Herba Corydalis Bungeanae, Radix Sophorae Flavescentis, Rhizoma Coptidis, Herba Artemisiae Scopariae, Radix Stephaniae hemandifoliae share, and can play heat clearing away The effect of removing toxic substances, conditioning milch cow body effectively treats endometritis；Meanwhile, raw material mangosteen skin also rich in Procyanidin, procyanidin can strengthen blood capillary, tremulous pulse and vein blood vessel, and it has detumescenceization to become silted up Effect, the drag reduction of blood capillary and permeability improve, and make cell be easier to absorb nutrient useless with eliminating Thing, procyanidin can be with water solublity in scavenger cell film and fat-soluble free radical, it is suppressed that discharge some Enzyme goes to injure the process of capillary wall, and procyanidin suppression produces the enzyme that histamine needs, prevents generation group Amine, thus dephlogistication, with Herba Leonuri, Indigo Naturalis, Radix Rhapontici, Rabdosia rubescens, Herba Corydalis Bungeanae, Rhizoma Corydalis Decumbentis, you Blue common effect can play the effect of the effect well restored menstrual flow and invigorated blood circulation, and dredges the blood vessels of affected part silt resistance, Make effective ingredient preferably arrive affected part simultaneously, accelerate curative effect；Each material effective component is comprehensively worked in coordination with Effect, can effectively treat cow endometritis.In raw material, mangosteen skin, Herba Leonuri, Entermorpha also have profit The effect of urine toxin expelling, can promote that Contents in Cows endotoxin is discharged by urine, accelerates to remove Contents in Cows endotoxin, Strengthen milch cow autoimmunity.

The invention have the benefit that and provide a kind of injection treating cow endometritis and system thereof Preparation Method, this injection is pure Chinese medicinal preparation, can safely and effectively treat cow endometritis, it is to avoid A large amount of use antibiotic medicines make milch cow produce drug resistance affects mass effect, effectively ensure that the food of milk Product safety.Specifically:

(1) for the cause of disease of cow endometritis, theoretical in conjunction with traditional Chinese veterinary medicine, the injection of the present invention Use anti-inflammation, heat-clearing and toxic substances removing, restore menstrual flow and invigorate blood circulation method treatment cow endometritis, as effectively In the raw material of the Chinese medicine extract of composition, mangosteen skin, Indigo Naturalis, Rabdosia rubescens, Entermorpha, Eucalyptus globulus Labill, Radix Sophorae Flavescentis, Rhizoma Coptidis, Herba Artemisiae Scopariae, Radix polygoni divaricati can well play the effect of anti-inflammation, and effectively suppression is killed such as Portugal The pathogenic bacterium such as grape coccus, streptococcus, escherichia coli, corynebacterium, effectively treat cow endometritis, Thus reduce the use of antibiotic, wherein, in mangosteen skin rich in tannic acid to staphylococcus, epidermis Portugal Grape coccus etc. has good antibacterial action, can affect biomembranous formation；Mangosteen skin and Herba Leonuri, green grass or young crops Black pigment used by women in ancient times to paint their eyebrows, Herba Artemisiae Scopariae, Radix Rhapontici, Rabdosia rubescens, Herba Corydalis Bungeanae, Radix Sophorae Flavescentis, Rhizoma Coptidis, Herba Artemisiae Scopariae, Radix Stephaniae hemandifoliae share, Ke Yiqi To the effect of heat-clearing and toxic substances removing, conditioning milch cow body effectively treats endometritis；Meanwhile, raw material mangosteen skin Also rich in procyanidin, procyanidin can strengthen blood capillary, tremulous pulse and vein blood vessel, and it has detumescence Changing the effect become silted up, the drag reduction of blood capillary and permeability improve, make cell be easier to absorb nutrient with Getting rid of refuse, procyanidin can be with water solublity in scavenger cell film and fat-soluble free radical, it is suppressed that release Putting some enzyme and go to injure the process of capillary wall, procyanidin suppression produces the enzyme that histamine needs, prevents Generation histamine, thus dephlogistication, with Herba Leonuri, Indigo Naturalis, Radix Rhapontici, Rabdosia rubescens, Herba Corydalis Bungeanae, summer Nothing, Radix Stephaniae hemandifoliae act on the effect that can play the effect well restored menstrual flow and invigorated blood circulation jointly, the silt resistance of dredging affected part Blood vessels, make effective ingredient preferably arrive affected part simultaneously, accelerate curative effect；Each material effective component is combined Close synergism, can effectively treat cow endometritis.

(2) in the raw material of Chinese medicine extract of the present invention, mangosteen skin, Herba Leonuri, Entermorpha also have diuresis toxin expelling Effect, can promote that Contents in Cows endotoxin is discharged by urine, accelerate remove Contents in Cows endotoxin, reduce Contents in Cows endogenous cause of ill inflammation and the blood toxicity that produces, prevent the generation of complication, strengthen milch cow autoimmune Power, further ensures that milk milk products quality, reduces the content of the harmful components such as milk drug residue material.

(3) preparation method of the injection of the present invention is simple, suitable a large amount of productions, and is extracting mangosteen skin In effective ingredient and follow-up preparation process thereof, it is to avoid high-temperature heating means, protect in mangosteen skin is former The effective ingredient such as anthocyanidin, it is ensured that its action effect.

Detailed description of the invention

According to following embodiment, the present invention be may be better understood.But, those skilled in the art holds Readily understood, concrete material proportion, process conditions and result thereof described by embodiment are merely to illustrate this Invention and should be without limitation on the present invention described in detail in claims yet.

Embodiment 1

A kind of injection treating cow endometritis, comprises 20g mannitol in 1000ml injection, 60g sodium chloride, 50g Chinese medicine extract；Make described Chinese medicine extract raw material composition and parts by weight be: Herba Leonuri 10 parts, mangosteen skin 15 parts, Entermorpha fresh goods 7 parts, Indigo Naturalis 6 parts, Eucalyptus globulus Labill 8 parts, Radix Sophorae Flavescentis 4 Part, Rhizoma Coptidis 8 parts, Herba Artemisiae Scopariae 3 parts, Radix polygoni divaricati 6 parts, Radix Stephaniae hemandifoliae 1 part, Rhizoma Corydalis Decumbentis 4 parts, Radix Rhapontici 5 Part, Rabdosia rubescens 6 parts, Herba Corydalis Bungeanae 5 parts, Radix Glycyrrhizae Preparata 7 parts.

The preparation method of the injection of above-mentioned treatment cow endometritis, comprises the following steps:

(1) Herba Leonuri, Indigo Naturalis, Eucalyptus globulus Labill, Radix Sophorae Flavescentis, Rhizoma Coptidis, Herba Artemisiae Scopariae, Radix polygoni divaricati, Radix Stephaniae hemandifoliae, summer Nothing, Radix Rhapontici, Rabdosia rubescens, Herba Corydalis Bungeanae, the broken 50 mesh mixed-powders that obtain of Radix Glycyrrhizae Preparata, the addition of gained mixed-powder The water soaking that gross mass multiple is 5 times 1 hour, slow fire boiling 1 hour, cross 500 mesh drainage screens after decoction Obtain filtrate, the again water soaking of addition mixed-powder gross mass multiple 5 times 0.5 hour, slow fire boiling 1 Cross 500 mesh drainage screens after hour and obtain filtrate, merge twice gained filtrate and be cooled to room temperature, standby；

(2) after mangosteen skin and Entermorpha fresh goods are cleaned, addition step (1) gained filtrate is squeezed the juice, and stands 1 little Filtered to get filtrate time after A；

(3) step (2) gained filtrate A cryoconcentration (less than 40 DEG C) to relative density be 1.35 ± The thick paste of 0.02 (30 DEG C), gained thick paste vacuum lyophilization, gained solid micronizing obtains 1000 mesh Powder is Chinese medicine extract；

(4) weigh the Chinese medicine extract of 50g step (3) gained, add 20g mannitol and 60g chlorine Change sodium, add the stirring of 200ml water for injection and make it dissolve, inject water to mixed solution further total Volume 1000ml, mixed solution is added homogenizer is carried out homogeneous, homogeneous temperature 15 ± 2 DEG C, the time Within 13 ± 2 minutes, obtaining homogeneous liquid, regulation pH value is 7.0, further obtains with 0.22 μm filter fine straining Filtrate；

(5) to step (4) gained filtrate ultraviolet sterilization 25 ± 5 minutes, gained liquid subpackage is become 20ml/ Injection and get final product.

Embodiment 2

A kind of injection treating cow endometritis, comprises 20g mannitol in 1000ml injection, 60g sodium chloride, 50g Chinese medicine extract；Herba Leonuri 15 parts, mangosteen skin 20 parts, Entermorpha fresh goods 12 parts, Indigo Naturalis 9 parts, Eucalyptus globulus Labill 10 parts, Radix Sophorae Flavescentis 7 parts, Rhizoma Coptidis 10 parts, Herba Artemisiae Scopariae 5 parts, Radix polygoni divaricati 9 parts, Radix Stephaniae hemandifoliae 3 parts, Rhizoma Corydalis Decumbentis 8 parts, Radix Rhapontici 9 parts, Rabdosia rubescens 10 parts, Herba Corydalis Bungeanae 9 parts, Radix Glycyrrhizae Preparata 10 Part.

The preparation method of the injection of embodiment 2 is same as in Example 1.

Embodiment 3

1. treat an injection for cow endometritis, 1000ml injection comprises 20g manna Alcohol, 60g sodium chloride, 50g Chinese medicine extract；Make raw material composition and the weight portion of described Chinese medicine extract Number is: Herba Leonuri 12 parts, mangosteen skin 18 parts, Entermorpha fresh goods 10 parts, Indigo Naturalis 7 parts, Eucalyptus globulus Labill 9 parts, Radix Sophorae Flavescentis 5 parts, Rhizoma Coptidis 9 parts, Herba Artemisiae Scopariae 4 parts, Radix polygoni divaricati 7 parts, Radix Stephaniae hemandifoliae 2 parts, Rhizoma Corydalis Decumbentis 6 parts, Radix Rhapontici 7 parts, Rabdosia rubescens 8 parts, Herba Corydalis Bungeanae 7 parts, Radix Glycyrrhizae Preparata 8 parts.

The preparation method of the injection of embodiment 3 is same as in Example 1.

Embodiment 4 extracorporeal bacteria inhibitor test

1, experimental strain: staphylococcus aureus (ATCC6538)

2, bacterium culture medium: nutrient agar and nutrient broth medium.

3, bacteriostatic experiment divides three experimental grouies and a matched group, and experimental group uses embodiment 1 to 3 respectively Injection, wherein, experimental group A is the injection of embodiment 1, and experimental group B is the note of embodiment 2 Penetrating liquid, experimental group C is the injection of embodiment 3, and matched group uses normal saline.

4, specific experiment step:

(1) prepared by susceptibility sense sheet: the circle of 20 diameter 6mm made by double-round board qualitative filter paper card punch The scraps of paper are put in the penicillin empty bottle that cleaning is dried, and bottleneck is wrapped up with monolayer kraft paper, 121 DEG C of autoclavings 30min, then through 100 DEG C of drying, airtight saves backup.The most respectively the scraps of paper are soaked in reality Execute 2h in the injection of example 1 to 3 and normal saline, after liquid is absorbed by the scraps of paper completely, take out filter paper Sheet, it is standby that susceptibility sheet is made in 50 DEG C of drying, and every kind of liquid prepares 5 susceptibility sense sheets；

(2) bacterium solution is prepared: staphylococcus aureus strain is placed in 10ml nutrient broth medium, 37 DEG C Cultivate 18h；Take 0.9% aseptic sodium chloride solution that culture fluid 1ml adds 9ml, use 10 times to be incremented by dilute Interpretation of the law, is diluted to 105～106cfu/ml by bacterium solution, standby；

(3) the test bacterium solution that above-mentioned steps (2) prepares uniformly is inoculated in mannitol sodium chloride agar to put down On plate, left at room temperature 5min, the 20 susceptibility sense sheets prepared in step (1) are laid in and are inoculated with gold On the agar plate of Staphylococcus aureus, flat board is inverted in incubator after 37 DEG C of cultivation 24h, survey Measuring the antibacterial circle diameter (mm) of each susceptibility sheet, calculate the antibacterial circle diameter meansigma methods of each group, above operation is equal For sterile working.

5, experimental observation is measured:

Observe: represent with "-" without inhibition zone；

Antibacterial circle diameter ＜ 10mm is the most antibacterial, with "+" represent；

Antibacterial circle diameter 10～14mm is that moderate is antibacterial, represents with " ++ "；

Antibacterial circle diameter ＞ 14mm is that height is antibacterial, represents with " +++ "；

	Negative control	Experimental group A	Experimental group B	Experimental group C
					Staphylococcus aureus	-	+++	+++	+++

Test result indicate that, the injection of the present invention Main Pathogenic Bacteria golden yellow Fructus Vitis viniferae to endometritis Coccus has preferable inhibitory action, may be used for treating cow endometritis.

Embodiment 5 therapeutic effect is tested

Choose parity, age, the lactogenic moon close milch cow 60 suffering from endometritis, be randomly divided into reality Test group A, experimental group B and tri-experimental grouies of experimental group C, respectively the note of trial edition inventive embodiments 1 to 3 Penetrate liquid, within 1st, inject 2 times, the injection 20ml of the shot present invention, after 6 days, observe its curative effect, The standard of curative effect evaluation is:

Cure: intrauterine is discharged without rheuminess thing and pus, and uterus recovers normal substantially, examination per rectum Time uterine contraction to normally, microscopy leukocyte is normal；

Effective: intrauterine rheuminess thing and pus output significantly reduce；

Take a turn for the better: without any therapeutic effect or aggravation.

Efficacy result statistics is as follows:

	Experimental group A	Experimental group B	Experimental group C
				Cure (head)	10	14	12
Take a turn for the better (head)	9	6	7
				Invalid (head)	1	0	1
Effective percentage (%)	95	100	95

Result of the test shows: the injection of the present invention is evident in efficacy in terms for the treatment of cow endometritis, Effective percentage is up to more than 95%, is the ideal medicament for the treatment of cow endometritis.

Embodiment of above only for technology design and the feature of the present invention are described, its object is to allow and is familiar with this The people of item technology understands present invention and is carried out, and can not limit the scope of the invention with this, All equivalence changes done according to spirit of the invention or modification, all should contain the protection model in the present invention In enclosing.

Claims (5)

1. the injection treating cow endometritis, it is characterised in that: 1000ml injection comprises 20g mannitol, 60g sodium chloride, 50g Chinese medicine extract；Make described Chinese medicine extract raw material composition and Parts by weight are: Herba Leonuri 10-15 part, mangosteen skin 15-20 part, Entermorpha fresh goods 7-12 part, Indigo Naturalis 6-9 Part, Eucalyptus globulus Labill 8-10 part, Radix Sophorae Flavescentis 4-7 part, Rhizoma Coptidis 8-10 part, Herba Artemisiae Scopariae 3-5 part, Radix polygoni divaricati 6-9 part, Radix Stephaniae hemandifoliae 1-3 part, Rhizoma Corydalis Decumbentis 4-8 part, Radix Rhapontici 5-9 part, Rabdosia rubescens 6-10 part, Herba Corydalis Bungeanae 5-9 part, process Radix Glycyrrhizae 7-10 part.

A kind of injection treating cow endometritis, it is characterised in that: Make described Chinese medicine extract raw material composition and parts by weight be: Herba Leonuri 10 parts, mangosteen skin 15 parts, Entermorpha fresh goods 7 parts, Indigo Naturalis 6 parts, Eucalyptus globulus Labill 8 parts, Radix Sophorae Flavescentis 4 parts, Rhizoma Coptidis 8 parts, Herba Artemisiae Scopariae 3 parts, acid is not Slip root 6 parts, Radix Stephaniae hemandifoliae 1 part, Rhizoma Corydalis Decumbentis 4 parts, Radix Rhapontici 5 parts, Rabdosia rubescens 6 parts, Herba Corydalis Bungeanae 5 parts, process 7 parts of Radix Glycyrrhizae.

A kind of injection treating cow endometritis, it is characterised in that: Make described Chinese medicine extract raw material composition and parts by weight be: Herba Leonuri 15 parts, mangosteen skin 20 parts, Entermorpha fresh goods 12 parts, Indigo Naturalis 9 parts, Eucalyptus globulus Labill 10 parts, Radix Sophorae Flavescentis 7 parts, Rhizoma Coptidis 10 parts, Herba Artemisiae Scopariae 5 parts, acid Do not slip root 9 parts, Radix Stephaniae hemandifoliae 3 parts, Rhizoma Corydalis Decumbentis 8 parts, Radix Rhapontici 9 parts, Rabdosia rubescens 10 parts, Herba Corydalis Bungeanae 9 parts, Radix Glycyrrhizae Preparata 10 parts.

A kind of injection treating cow endometritis, it is characterised in that: Make described Chinese medicine extract raw material composition and parts by weight be: Herba Leonuri 12 parts, mangosteen skin 18 parts, Entermorpha fresh goods 10 parts, Indigo Naturalis 7 parts, Eucalyptus globulus Labill 9 parts, Radix Sophorae Flavescentis 5 parts, Rhizoma Coptidis 9 parts, Herba Artemisiae Scopariae 4 parts, acid is not Slip root 7 parts, Radix Stephaniae hemandifoliae 2 parts, Rhizoma Corydalis Decumbentis 6 parts, Radix Rhapontici 7 parts, Rabdosia rubescens 8 parts, Herba Corydalis Bungeanae 7 parts, process 8 parts of Radix Glycyrrhizae.

5. the preparation side of a kind of injection treating cow endometritis as described in Claims 1-4 Method, it is characterised in that comprise the following steps:

(1) Herba Leonuri, Indigo Naturalis, Eucalyptus globulus Labill, Radix Sophorae Flavescentis, Rhizoma Coptidis, Herba Artemisiae Scopariae, Radix polygoni divaricati, Radix Stephaniae hemandifoliae, Rhizoma Corydalis Decumbentis, Radix Rhapontici, Rabdosia rubescens, Herba Corydalis Bungeanae, the broken 50 mesh mixed-powders that obtain of Radix Glycyrrhizae Preparata, gained mixed-powder addition gross mass The water soaking that multiple is 5 times 1 hour, slow fire boiling 1 hour, cross 500 mesh drainage screens after decoction and obtain filtrate, The water soaking of addition mixed-powder gross mass multiple 5 times 0.5 hour again, slow fire boiling crossed 500 after 1 hour Mesh drainage screen obtains filtrate, merges twice gained filtrate and is cooled to room temperature, standby；

(2) after mangosteen skin and Entermorpha fresh goods are cleaned, addition step (1) gained filtrate is squeezed the juice, and stands 1 hour After filter to get filtrate A；

(3) step (2) gained filtrate A cryoconcentration (less than 40 DEG C) is 1.35 ± 0.02 to relative density The thick paste of (30 DEG C), gained thick paste vacuum lyophilization, gained solid micronizing obtains 1000 mesh powder i.e. For Chinese medicine extract；

(4) weigh the Chinese medicine extract of 50g step (3) gained, add 20g mannitol and 60g chlorination Sodium, adds the stirring of 200ml water for injection and makes it dissolve, inject water to mixed solution cumulative volume further 1000ml, mixed solution is added homogenizer is carried out homogeneous, homogeneous temperature 15 ± 2 DEG C, the time 13 ± 2 points Clock obtains homogeneous liquid, and regulation pH value is 7.0, further obtains filtrate with 0.22 μm filter fine straining；

(5) to step (4) gained filtrate ultraviolet sterilization 25 ± 5 minutes, gained liquid subpackage is become 20ml/ Injection and get final product.