RU2700081C1 - Preparation "berkana" for prevention and treatment of endometritis in cows and a method for production thereof - Google Patents

Abstract

FIELD: agriculture.

SUBSTANCE: invention refers to agriculture, namely to veterinary gynecology. Disclosed is a preparation for preventing and treating endometritis in cows in the form of a plant extract of raw material from birch wood processing wastes in a water-dispersed state, with the following ratio of components: raw material from birch wood processing wastes – 18–22 wt%, water – balance. Raw material contains birch bark and bast lubes at the following ratio: birch bark – 17–23 wt%, birch bast – 77–83 wt%. Also disclosed is a method for preparing a preparation for preventing and treating endometritis in cows by extraction, including grinding raw materials, pouring with distilled water and treatment with frequency vibrations. Grinding is performed to 1–2 mm fraction, vibration treatment is carried out using oscillations in infrasound frequency range. Duration of vibration treatment is 15–16 days to obtain water-dispersed with particle size of 15–27 mcm vegetal extract of raw material from wastes of birch wood processing. Then, the extract is packaged in sterile conditions.

EFFECT: invention enables to obtain an effective herbal preparation for treating cows' endometritis.

3 cl, 6 tbl, 6 dwg

Description

The invention relates to agriculture, namely to medicinal preparations based on plant materials used in veterinary gynecology, and methods for their preparation, and can be used in agricultural enterprises during preventive and therapeutic measures in veterinary medicine.

The most important factor determining the profitable management of animal husbandry is the formation of a healthy herd of animals, taking into account the latest achievements of science and best practices, and the production of environmentally friendly meat and milk without antibiotics.

The pathomorphological picture of lesions of vital organs in chronic diseases or with age-related degeneration is diverse, but it contains one common element - a violation of the stability of cell membranes, their biological wear. A universal role in the pathogenesis of membrane-cell lesions belongs to free radicals, which cause the destruction of biopolymers. An increase in the content of free radicals in the body is accompanied by a decrease in the total antioxidant activity, in particular, a decrease in the antioxidant activity of lipid complexes. Some substances, inhibitors of free radical processes that do not have antioxidant activity per se, are able to increase the antioxidant properties of lipids and enhance the radioprotective effect of endogenous antioxidants. Inhibitors of radicals, exogenous antioxidant drugs in optimal doses make up for the deficiency of endogenous antioxidants and reduce the scale of lesions associated with free radical reactions.

In this regard, the development of specialists from the Bashkir Scientific Research Institute of Agricultural Sciences and Agroveit LLC for the prevention and treatment of inflammatory processes in the body of cows is relevant.

A known drug that is used in veterinary gynecology, which includes antibiotics and various targeted supplements of non-plant origin (RU 2455992, publ. 20.07.2012, RU 2333759, publ. 09/20/2008, RU 2611039, publ. 02.20.2017).

It is known that antibiotics that enter the human body through the food chain: “animal-production-man” pose a serious danger to his health (Usha B.V., Kalnitskaya OI. Control of antibiotic residues in raw materials and animal products // “Modern problems of veterinary pharmacology and toxicology. ”The Second Congress of Veterinary Pharmacologists and Toxicologists of Russia. Kazan. June 9-12, 2009. Materials of the Congress. - Kazan, 2009, p. 11-14). The most important factor determining the profitable management of animal husbandry is the formation of a healthy herd of animals, taking into account the latest achievements of science and best practices, and the production of environmentally friendly meat and milk without antibiotics. For this reason, an important factor is the creation of such drugs that contain components that are harmless to humans.

Known preparations containing a valuable biologically active substance betulin, which is obtained by hydrolysis from birch bark (RU 2291684, publ. 20.01.2007, RU 2319497, publ. 20.03.2008, RU 2251407, publ. 10.05.2005, RU 2252775, publ. 27.05 .2005) and is used for various therapeutic purposes, however, they have not been used to date in veterinary gynecology.

Betulin is a valuable biologically active resinous substance of white color, which is obtained from birch bark (http://www.poznavavka.org/anatomiya-i-meditsina/betulin-lekarstvo-ot-samov-prirodvi-s-raznostoronnim-devstviem/) .

The use of an aqueous extract of birch wood processing waste as a therapeutic preparation for cows from open sources of information is not known.

The group of inventions solves a single technical problem, which consists in obtaining safe and inexpensive betulin in production - containing a herbal preparation from waste products from birch wood, which has pronounced pharmacological properties and high efficiency in the prevention and treatment of endometritis in cows as a result of influencing the dynamics of the body's immunobiological parameters cows by eliminating disorders in the organs of their reproductive system.

The technical result consists in obtaining the specified drug when creating conditions that ensure the high solubility of the substance of betulin in the aquatic environment during the production of the aqueous extract, its penetration through the cell membranes of a living cell and a high degree of biological activity, which allows the drug to influence the cause of the disease, and not just remove its symptoms.

The technical result is achieved by the fact that the proposed drug for the prevention and treatment of endometritis in cows in the form of a plant extract of raw materials from waste processing of birch wood in a water-dispersed state in the following ratio of components, wt. %:

raw materials from birch wood processing waste 18-22

water rest,

while the feed contains birch bark and birch bast in a ratio, wt.%:

birch birch 17-23 birch bast 77-83

The technical result is also achieved by the fact that the method of obtaining the drug for the prevention and treatment of endometritis in cows by extraction involves grinding the raw material, pouring with distilled water and processing with frequency fluctuations, and is characterized in that birch bark and birch bast from waste products from birch wood are used as raw materials in the ratio , wt%:

birch birch 17-23 birch bast 77-83

and grinding is carried out to a fraction of 1-2 mm, vibration processing is carried out using vibrations in the infrasonic frequency range, the duration of the vibration processing is 15-16 days to obtain a water-dispersed plant extract of raw materials from birch wood processing waste with a particle size of 15-27 microns in the following ratio components, wt. %:

raw materials from birch wood processing waste 18-22

water rest

and in sterile conditions packaged in containers.

In addition, the oscillation frequency during infrasonic processing is 1-4 Hz.

A known method of producing betulin from birch bark, including crushing the bark, extraction with alcohol, separation of the solution, followed by removal of solvent from it (SU 382657, publ. 05.23.1973). The crust is crushed in the presence of alkali, then hot 85-88% isopropyl alcohol is added with stirring at a temperature of 60-70 ° C, the solvent is separated off, water is added to the obtained extract, and the betulin precipitated is separated off.

A known method of producing betulin from birch birch bark, including its activation at a temperature of 180-260 ° C, a pressure of 2-5 MPa for 60-300 s, alkali treatment and extraction with alcohol (RU 2131882, publ. 06/20/1990).

A disadvantage of the known methods is the low pharmacological value of the final product, because in the process of hydrolysis, a number of extraactive substances are lost. The output of the most basic active substance of betulin is only 10-30%.

The closest in technical essence to the invention is a bioresonance method of obtaining a drug for the treatment of burns and wounds by extraction to obtain an aqueous extract of a plant substance - Siberian larch (RU 2470653, publ. 27.12.2012).

The specified method includes processing the sawn parts of the trunk of a larch, immersed in distilled water, by fluctuations of a given frequency. The emitter was lowered into the bath and the frequencies corresponding to the frequencies of Siberian larch (from 10 to 30 Hz) were selected experimentally on the generator. Received an aqueous extract of Siberian larch.

The resulting preparation contains dihydroquercytin, a complex of polysaccharides, flavonoids, components of essential oils.

However, it does not contain betulin and other biologically active substances and chemical elements that are found only in live birch (lat Betula pendula Roth.), Necessary for the treatment and prevention of gynecological diseases of animals, namely, endometritis in cows due to its unique properties of penetration through cell membranes of a living cell.

It is known that birch bark tissue consists of an inner layer - bast, and an outer layer called birch bark. The physiological function of the bast is to transfer assimilation products and serves to store reserve nutrients.

The birch bark and bast of most species of birch contain triterpenoid betulin, the content of which varies from 5 to 27% from different types of birches. Betulin has a wide spectrum of biological activity. In addition, the content of essential oils in the birch bark reaches 3-5%, the main components of which are sesquiterpenoids, resinous substances, alkaloids, ascorbic acid, flavonoids and higher fatty acids, derivatives of dammaran, coumarins, tannins and flavonoids up to 40%. which are biologically active substances with high pharmacological properties (https://cvberleninka.ru/article/n/vvdelenie-betulina-iz-berestv-berezv-i-izuchenie-eqo-fiziko-himicheskih-i-farmakologicheskih -svoystv).

To obtain a highly effective betulin-containing medicinal product, it is necessary to create conditions that ensure the high solubility of the substance of betulin in the aqueous medium during the production of the aqueous extract, its penetration through the cell membranes of the living animal cells and a high degree of biological activity, which would allow the drug to influence the cause of the disease, and not only relieve its symptoms.

In this regard, a method was developed for producing a drug for the prophylaxis and treatment of endometritis in cows by extraction, including grinding of raw materials, pouring with distilled water and processing with frequency vibrations, in which birch bark and birch bast from waste products from birch wood are used as raw materials in the ratio, wt . %:

birch birch 17-23 birch bast 77-83

Grinding is carried out to a fraction of 1-2 mm, vibration processing is carried out using vibrations in the infrasonic frequency range, the duration of the vibration processing is 15-16 days to obtain a water-dispersed plant extract from birch wood processing wastes with a particle size of 15-27 microns in the following ratio of components, wt. %:

raw materials from birch wood processing waste 18-22

water rest

and in sterile conditions packaged in containers.

Grinding raw materials to a fraction of 1-2 mm allows you to speed up the process of destruction of raw materials, because a fraction of not more than 2 mm helps to accelerate the destruction and increase the yield of useful components.

It has been experimentally proved that from the point of view of biologically active betulin content and chemical composition, the most optimal is the raw material containing birch bark and birch bast in the ratio (17-23) to (77-83), respectively. This ratio of components in raw materials allows you to get the maximum yield of the final product at a low cost of its manufacture.

If the specified ratio is not maintained, then the yield of highly effective plant extract in the composition of the drug is reduced. Moreover, in the composition of the extract, the raw material should not exceed 22 wt. %, but not to be less than 18 wt. %, which is explained by the fact that the yield of biologically active betulin is quite high and amounts to 68-85%.

It has been experimentally proved that biologically active substances in sufficient volumes are transferred to the final product if the vibration processing is carried out by means of vibrations in the frequency infrasonic range, and the duration of this processing is 15-16 days.

The maximum effect is observed when the oscillation frequency during infrasonic processing is 1-4 Hz, which is confirmed by the calculation of the interference pattern created by the infrasonic waves in order to provide the necessary effect of vibrations of a given length on a specific object. In this case, we selected the wavelength, “consonant” (resonant) wave of the plant - birch, which serves as a raw material for the preparation.

The specified method is simple, cheap, technologically advanced and harmless, all operations are easily feasible, because production waste was used and the use of concentrated acids and alkalis was excluded.

The invention is illustrated by the following figures.

In FIG. 1 presents table 1, which shows the yield of betulin depending on the content and composition of the raw materials upon receipt of the drug.

In FIG. 2 presents table 2, showing the chemical composition of the water-dispersed extract.

In FIG. 3 - table 3. illustrating the results of the selection of a therapeutic dose of the drug for the treatment of cows with endometritis.

In FIG. 4 - table 4, which shows the content of leukocytes in the blood of cows of the experimental group where :.

L - white blood cells,

M - monocytes,

B - basophils,

E - eosinophils,

Yu - young

P - stab

C - segmented.

In FIG. 5 presents table 5 with indicators of the phagocytic activity of neutrophils in the blood of cows of the experimental group.

In FIG. 6 is a table 6 illustrating the duration of uterine involution in cows, depending on the use of therapeutic agents and the benefits of the drug.

A preparation is proposed that contains betulin, which is present in the aqueous extract of waste products from birch wood and is an effective, safe and cheap herbal medicine for treating cows, which is especially valuable from the point of view of maintaining the health of a person eating cow meat and milk.

No similar drugs were found in the practice of veterinary gynecology.

It has been experimentally proved that from the point of view of biologically active betulin content and chemical composition, the most optimal is the raw material containing birch bark and birch bast in the ratio (17-23) to (77-83), respectively.

If the specified ratio is not maintained, then the yield of highly effective plant extract in the composition of the drug is reduced. Moreover, in the composition of the extract, the raw material should not exceed 22 wt. %, but not to be less than 18 wt. %, which is explained by the fact that the yield of biologically active betulin is quite high and amounts to 68-85% (table 1 in Fig. 1).

The content of chemical elements and ions in the obtained water-dispersed extract is presented in table 2 in FIG. 2.

Table 2 shows that the product contains such chemical elements as silicon zinc, magnesium and iron and they are in a water-soluble state, therefore they are easily absorbed by the animal’s body, which is positive from the point of view of the pharmacological properties of the drug ..

Get the drug by the method described in the example. Preparation Example

The components of the raw material were waste products from birch wood: birch birch - 20%, wt. and birch bast - 80% wt.

The raw materials were crushed on a crusher-grinder ВХ-42 to a fraction of 1-2 mm and placed in a bathtub with a volume of 500 l.

Distilled water was poured into the bath with crushed raw materials. Then, an electrocoil - emitter was placed over the bathtub, made by wrapping a copper wire with a diameter of 2 mm around a plastic cone with a base diameter of 15 mm and with a tip diameter of 1 cm, which was connected to a PNG-4-type frequency oscillation source. Set a constant oscillation frequency of 4 Hz.

For 15 days, the raw material was under the influence of constant vibration created by infrasonic frequency oscillations.

At this time, the extraction of substances in the raw materials, which gradually turned into a water-dispersed state, was carried out. The extract had the composition: raw materials - 20% wt, water - the rest.

The resulting water-dispersed extract was filtered on a membrane filter with cartridges from 14 microns to 27 microns.

The filtered product under sterile conditions was packaged in a container of 100 and 200 ml.

Below is an example of testing the therapeutic effect of the drug in an experimental group of sick cows, which was carried out at two agricultural enterprises of the Republic of Bashkortostan.

The study subjected 30 cows of black-motley breed at the age of 4 years. The data of biochemical blood tests allowed us to state that in cows of the experimental group there was a significant increase in albumin by 5.1% and a parallel increase in total protein content by 6.1%, which indicates the presence of an inflammatory process in the uterus - postpartum endometritis.

The therapeutic effect of the preparation according to the invention is based on the activation of general and local immunity in animals. Studies have been conducted aimed at studying the effect of the drug on the dynamics of the immunobiological parameters of the body of cows to eliminate disorders in the organs of the reproductive system.

The results of choosing the optimal therapeutic dose of the drug for the treatment of cows with endometritis are given in table. 3 in FIG. 3.

As follows from table 3, the optimal dosage of the drug is 10.0 cm ³ with a single injection for 6 days of treatment.

For the treatment of cows, the drug was injected intramuscularly into the sub-caudal fossa at a dose of 10.0 cm ³ once a day for 6 days.

Animals were monitored daily until completely cured.

To assess the immunobiological status of the animal organism, methods of clinical laboratory diagnostics, including a number of biochemical and morphological blood tests, were used. Blood samples were taken from all cows on the day the experiment began and one month after treatment, when the uterus returned to normal.

After a course of injections with the drug, a decrease in the level of albumin was observed, the amount of which decreased after treatment by almost 6%, which indicates the normalization processes in the focus of inflammation of the animal organism.

After treatment, a marked change in the ratio of globulin fractions of proteins was noted. At the same time, the content of α-globulins significantly increased by 54.5%, which characterizes the course of the inflammatory process in the body, provided with a normally functioning immune system, which predicts a favorable outcome. Under the action of the drug, the β-globulin fraction of blood serum did not undergo changes, which indicates the normal functioning of the kidneys.

Large changes were noted in the γ-globulin fraction of proteins. The difference between the levels of γ-globulins in experimental cows before and after treatment reached 22.5%.

In the blood serum of cows of the experimental group, a slight increase in IgG class immunoglobulins (by 2.9%) and a reliable increase in Ig M class (by 18.1%) were observed. Along with this, a slight increase in the level of total lipids in cows after treatment was found to be 5.8%, while the fluctuation in lipid content did not exceed normative indicators, which indicates a good physiological state of the liver, where these fat fractions are synthesized.

Although the fluctuation of cholesterol in the blood of experimental animals was reliable - 16.8%, it did not exceed the minimum and maximum values of this indicator in the norm. The drug did not negatively affect the function of the liver cells where cholesterol is synthesized.

The content of glucose, hemoglobin in the blood of animals corresponded to the physiological norm. This indicates that the use of the drug did not violate the carbohydrate metabolism in the body of cows.

Indications of hematocrit in all animals did not go beyond the physiological norm.

The blood erythrocyte count in the experimental group of cows was 7.2 × 10 ¹² / L ± 2.10 before treatment and 6.7 × 10 ¹² / L ± 1.01 (P <0.05) after treatment.

Treatment of endometritis in cows with the drug positively affected erythropoiesis, as a result, the number of red blood cells in the blood significantly increased by 6.9% compared with the figures at the beginning of treatment.

The leukocyte formula has great prognostic and diagnostic value about the condition of the animal as a whole and the stage of the course of the disorder, and also allows you to assess the state of the immune reactivity of the body, to monitor the effectiveness of the therapy used.

After treatment of the animals, the number of leukocytes in their blood decreased by 12.4% compared with the leukocyte count at the beginning of the experiment, which is confirmed by the data of table 4 in FIG. four.

The drug possessed not only therapeutic, stimulating, but also corrective action in relation to the immune status, most of the indicators of which after treatment were within the physiological norm.

A significant decrease in the level of leukocytes and a slight decrease in red blood cells indicates a decrease in the inflammatory process in the uterus. Confirmation of this is an increase in the globulin fraction of proteins.

The number of neutrophils before and after treatment was within physiological values. However, after treatment, the content of young neutrophils significantly increased by 25.0% (P <0.05) and stab core - by 36.7% (P <0.01) while reducing the number of segmented by 9.5% (P <0.002) . Such indicators characterized the presence of a regenerative nuclear shift, which indicated the neutrophilic phase of the fight against the inflammatory process in the body, accompanied by the release of neutrophils into the bloodstream, which is shown in Table 5 in FIG. 5.

The research results indicate that when treating endometritis with a drug in cows, the activity of blood-forming organs began to intensify, as a result, leukocyte counts changed, which indicated an increase in non-specific humoral and cellular immunity.

Quantitative indicators of most blood elements after undergoing treatment in animals were within the physiological norm.

The effectiveness of the drug was also confirmed by the fact that the control group of experimental animals, the treatment of which was carried out in the traditional way, the restoration of the reproductive functions of animals took longer, which follows from table 6 in FIG. 6.

Therapeutic experimental and clinical studies in the treatment of endometritis, as well as uterine involution in cows, suggest that the drug has a wide spectrum of antimicrobial action, which eliminates the need for a preliminary determination of the type or strain of the pathogen and accelerates the treatment process.

The speed of the therapeutic effect, as well as the absence of cumulative effects, makes it possible to carry out veterinary measures in a short time, eliminating the possibility of microbes getting used to the drug and the emergence of stable forms. In addition, the fungicidal and antiviral properties of the drug should be noted.

The use of the drug does not cause allergic reactions and side effects in cows.

Claims (12)

1. The drug for the prevention and treatment of endometritis in cows in the form of a plant extract of raw materials from waste products from birch wood in a water-dispersed state in the following ratio of components, wt. %: raw materials from birch wood processing waste 18-22 water rest, while the raw material contains birch bark and birch bast in the ratio, wt. %: birch birch 17-23 birch bast 77-83 2. A method of obtaining a drug for the prevention and treatment of endometritis in cows by extraction, including grinding raw materials, pouring with distilled water and processing with frequency fluctuations, characterized in that birch bark and birch bast from waste products from birch wood are used as raw materials in the ratio, wt. %: birch birch 17-23 birch bast 77-83, and grinding is carried out to a fraction of 1-2 mm, vibration processing is carried out using vibrations in the infrasonic frequency range, the duration of the vibration processing is 15-16 days to obtain a water-dispersed plant extract of raw materials from birch wood processing waste with a particle size of 15-27 microns in the following ratio components, wt. %: raw materials from birch wood processing waste 18-22 water rest and in sterile conditions packaged in containers. 3. The method of obtaining the drug according to claim 2, characterized in that the oscillation frequency during infrasonic processing is 1-4 Hz.

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