RU2470653C2 - Agent for burn and wound healing - Google Patents

Abstract

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to pharmaceutical industry, particularly to a medicine for treating depression. An agent for burn and wound healing containing aqueous Siberian larch extract prepared by bioresonance procedure, containing 0.002% of dihydroquercetin of certain particle size and water in certain proportions.

EFFECT: agent is effective for burn and wound healing; it is injectable.

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Description

The invention relates to medicine and the pharmaceutical industry, in particular to the production of a medicinal product, including dihydroquercetin, from natural raw materials not subject to chemical treatment, and can be used to treat wound surfaces, burns, etc.

Dihydroquercetin (DHA) - is a reference antioxidant. It has powerful anti-inflammatory and anti-allergenic properties, strengthens and restores connective tissue, helps lower cholesterol, enhances the action of many useful substances (vitamin C and vitamin E); strengthens blood vessels and capillaries, improves blood microcirculation, prevents blood clots, reduces inflammation in the prostate, strengthens the immune system. Protects the stomach and liver from harmful effects, activates the regeneration of the gastric mucosa. It has a pronounced prevention of the main diseases of aging: cancer, cardiovascular diseases, brain diseases, etc. It increases the resistance of body tissues to the damaging effects of excessive blood sugar, reduces the likelihood of developing diabetes, and also facilitates the course of developed forms. It has a positive effect on the nervous system, activates nervous processes.

Known antioxidant, capillary-protective, anti-inflammatory and antihistamine, which is used as a flavonoid compound - dihydroquercetin (RF patent No. 201441, class A61K 35/78, publ. 30.06.1994).

The dihydroquercetin obtained by the method described in the patent is very slightly soluble in water at room temperature, which makes it impossible to use it in injection practice.

Known phospholipid emulsion containing dihydroquercetin, at least one membrane-forming phospholipid, glycine and an aqueous solution of ethyl alcohol with an alcohol content of from 0.05 to 20%, protected by RF patent No. 2369383, cl. A61K 9/107; A61K 9/127; A61K 31/353; A61K 47/16; A61J 3/00; A61P 17/02, publ. 10/10/2009

To obtain a phospholipid emulsion, a solution of dihydroquercetin and phospholipid in ethyl alcohol and a dispersion medium, including a solution of glycine in deionized water, are prepared, the dispersion medium is untwisted until a stable rotation funnel is formed in it, the dihydroquercetin solution is introduced into the rotation funnel and the resulting mixture is divided into the dispersed phase concentrate and the dispersed phase Wednesday

A disadvantage of the known composition is that DHA is poorly soluble in water and sensitive to the action of metal ions, as a result of which injection is often impossible, and oral administration is fraught with a loss of activity during the passage of the gastrointestinal tract.

The closest technical solution to the claimed invention, selected as a prototype, is a gel balm with a wound healing effect, protected by RF patent No. 2381810, class. A61K 36/55, A61K 36/15, A61K 31/35, A61P 17/02, publ. 02/20/2010

The gel-balm contains dihydroquercetin, medical glycerin, linseed or cedar oil, chitosan water gel, taken in a certain ratio of components.

As the main disadvantage of the known funds can be noted poor solubility in water, as a result of which injection is often impossible.

The technical result consists in increasing the solubility of dihydroquercetin in water.

The specified technical result is achieved in that the agent for treating burns and wounds, including dihydroquercetin, contains an aqueous extract of Siberian larch obtained by the bioresonance method, containing 0.002% dihydroquercetin with particle sizes from 198 to 460 nm, and water with the following content of components, wt.% _:

Water extract of Siberian larch - 5-40

The values of the components of the funds listed above are optimal to achieve the maximum therapeutic effect.

An aqueous extract of Siberian larch is obtained by the bioresonance method. A wooden bath is made from Siberian larch, the trunk of the Siberian larch, sawn into several parts, is immersed in it. Distilled water is poured. The process is carried out using a low-frequency generator, for example, G6-36. A copper wire with a resistance of 8 to 15 Ohms is wound on the generator core. The emitter is lowered into the bath and empirically using the generator select the frequency corresponding to the frequencies of Siberian larch. Due to the coincidence of the phases, a resonance is obtained, which contributes to the release of intracellular fluid from Siberian larch into the water. Frequency range from 10 (-5 degrees) Hz to 30 Hz.

The aqueous extract can be stored for up to a year in a cool, dark place.

The aqueous extract of Siberian larch obtained by the bioresonance method is a dark brown foamy liquid with a pleasant smell. The full UV spectrum of the extract shows that it contains 0.002% dihydroquercetin, a complex of polysaccharides with polyphenolic compounds, terpene saponins, flavonoids with conjugated carbonyl and hydroxyl groups, and components of essential oils. All active substances of the Siberian larch aqueous extract obtained by the bioresonance method have a nanoparticle size, in particular, DHA from 198 to 460 nm, which increases the solubility of DHA in water and makes its injection possible. 10 minutes after injection of DHA, a 4-fold increase in plasma fluidity is noted. After 20-30 minutes, activity of red blood cells and white blood cells is noted, blood counts improve, peripheral blood microcirculation improves.

If we take water more than 95 wt.%, An aqueous extract of Siberian larch obtained by the bioresonance method, less than 5 wt.%, Then when using the product, the therapeutic effect is not achieved, which is confirmed by clinical trials.

If we take water less than 60 wt.%, An aqueous extract of Siberian larch obtained by the bioresonance method, more than 40 wt.%, Then as a result of using the drug, undesirable side effects and exacerbations of diseases are observed. This is confirmed by experimental studies on volunteers.

The quantitative content of the components of the tool is selected empirically.

Siberian larch wood extract obtained by other known methods (for example, by chemical methods) contains dihydroquercytin with a high molecular weight, which is the main obstacle to its injection and penetration through the stratum corneum.

Composition 1: aqueous extract of Siberian larch obtained by the bioresonance method, 40 ml, water - 60 ml.

Composition 2: aqueous extract of Siberian larch obtained by the bioresonance method - 5 ml, water - 95 ml.

The tool was prepared by simple mixing of the components. Packed in bottles of 10-100-200 ml, sterilized for 20 minutes at a temperature of 60 ° C. Sterility is confirmed by studies conducted in the bacteriological laboratory of MLPU "City Clinical Hospital No. 12" in Nizhny Novgorod.

The resulting agent is administered repeatedly in direct proportion to the severity of the disease. Treatment is carried out in courses of 20 injections.

The invention is illustrated by the following examples of the treatment of diseases in volunteers.

Example 1

Woman, 47 years old. The wound surface of the gums with suppuration after the extraction of the molar.

The pain effect decreased by 50-70% after injection of the proposed agent directly into the wound and tamponing of the wound with a swab moistened with an aqueous extract of Siberian larch obtained by the bioresonance method.

The course of treatment of the proposed drug (composition 1) was 3 days. DHA was administered in a dose of 0.5 ml intramuscularly daily. As a result, bleeding after tooth extraction stopped after plugging, and healing of the gums occurred the next day.

Example 2

Woman, 53 liters Fresh laceration of the thigh, 3 cm deep, area 15 cm ² .

The proposed agent was injected into the wound in an amount of 5 ml and a bandage was impregnated with an aqueous extract of Siberian larch obtained by the bioresonance method. The pain syndrome resolved after 5-7 minutes. After 3 days of daily dressings with an aqueous extract of Siberian larch obtained by the bioresonance method (composition 2), complete granulation of the wound without signs of scar was noted and the surface of the wound was leveled. In addition to dressings, 0.5 ml of the extract was administered intramuscularly daily.

Example 3

Man, 33 g. Chemical burn of 2 degrees. The burned areas were washed with distilled water and a gauze bandage moistened with an aqueous extract of Siberian larch obtained by the bioresonance method (composition 2). The dressing was changed to fresh every 4 hours. The pain decreased by 60-70% after the first dressing. After applying 3 dressings, the pain disappeared completely. On the second day, a bandage was applied in the morning and evening. On day 3, the wound was closed with a dry dressing, since persistent tissue regeneration was noted. All 3 days were administered the proposed drug intramuscularly in 0.5 ml.

Example 4

Male, 47 years old. Torn forearm wound with initial suppuration and swelling, area 12 cm ² . The extract was used on day 3 after injury (composition 1). A gauze dressing was moistened with the extract and applied to the wound after preliminary treatment of the wound with hydrogen peroxide. The next day, the effect of relieving swelling and pain, initial granulation was noted. Over the next 4 days, the procedure for applying a bandage soaked in an aqueous extract of Siberian larch obtained by the bioresonance method was repeated. On day 5, the dressing was no longer applied, since the wound was completely granulated without scarring.

Thus, the proposed tool for the treatment of burns and wounds, containing an aqueous extract of Siberian larch obtained by the bioresonance method, including dihydroquercetin, is an effective drug for intramuscular use due to its solubility in water and can be widely used in practical medicine.

Claims (1)

The treatment for burns and wounds, including dihydroquercetin, characterized in that the product contains an aqueous extract of Siberian larch obtained by the bioresonance method, containing 0.002% dihydroquercetin with particle sizes from 198 to 460 nm, and water with the following components, wt.%:
Water extract of Siberian larch 5-40 Water 60-95