CN103181958A - Chinese herbal medicine perfusate for treating endometritis of cow and preparation method thereof - Google Patents
Chinese herbal medicine perfusate for treating endometritis of cow and preparation method thereof Download PDFInfo
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Abstract
Chinese herbal medicine perfusate for treating endometritis of a cow is prepared by BPC (bulk pharmaceutical chemicals) in parts by weight as follows: 5-7 parts of leonurus, 4-6 parts of Chinese angelica, 2-4 parts of szechuan lovage rhizome, 2-4 parts of a baical skullcap root, 2-4 parts of safflower, 2-4 parts of rhubarb, 1-2 parts of a coptis root, 1-2 parts of mint and 1-2 parts of liquorice. The preparation method of the perfusate comprises the steps of weighing the nine Chinese herbal medicine decoction pieces in the weight ratio, grinding respectively into coarse Chinese herbal medicine powder; taking the coarse Chinese herbal medicine powder of the Chinese angelica, the szechuan lovage rhizome, the baical skullcap root, the rhubarb, the coptis root and the liquorice, adding water and performing decoction, then adding the leonurus, the safflower and the mint, performing the decoction for three times, combining three filtrates, subjecting the filtrates to alcohol precipitation after concentration, adjusting pH, performing activated carbon decoloration, using tween-80 to assist solubilization, adding an preservative quantificationally, stirring uniformly, filtering, filling and performing sterilization to obtain the Chinese herbal medicine perfusate for treating the endometritis of the cow. The perfusate provided by the invention can treat the endometritis of the cow effectively.
Description
Technical field
The present invention relates to the Chinese herbal medicine that a kind of milk cattle cultivating is used, be specifically related to a kind of Chinese herbal medicine infusion liquid for the treatment of cow endometritis and preparation method thereof.
Background technology
Cow endometritis is the common disease in the milch cow reproductive system, and sickness rate seriously influences reproductive capacity and the fertility performance of milch cow up to 20%-40%, causes the economic benefit of milk cattle cultivating industry to reduce, and hinders the sound development of dairy.Cow endometritis causes that milch cow is infertile, increases the tire spacing, reduces the output of milk, already causes enormous economic loss for milk cattle cultivating.The pathogen of cow endometritis is kind more than 80 nearly, mainly contains antibacterial, Mycoplasma, fungus and virus, and antibacterial mainly is based on staphylococcus and streptococcus.At present, the medicine for the treatment of cow endometritis is a lot, and adopting more is antibiotics class Western medicine, though have certain curative effect, but shortcoming is that Drug resistance and side effect are more serious, and it is residual to have chemicals in meat and milk, brings potential threat to human health.That Chinese herbal medicine has is natural, have no side effect, do not have that chemicals is residual, characteristics such as be difficult for developing immunity to drugs.Prior art discloses the Chinese herbal medicine for the treatment of cow endometritis, but its weak curative effect, cure rate is low, and the course for the treatment of is long and cost is high.
Summary of the invention
The objective of the invention is at the problems referred to above, a kind of Chinese herbal medicine infusion liquid that can effectively treat cow endometritis is provided.The present invention also aims to provide the preparation method of this Chinese herbal medicine infusion liquid.
Technical scheme of the present invention is: a kind of Chinese herbal medicine infusion liquid for the treatment of cow endometritis, it is to be made by following raw materials by weight proportions: Herba Leonuri 5-7 part, Radix Angelicae Sinensis 4-6 part, Rhizoma Chuanxiong 2-4 part, Radix Scutellariae 2-4 part, Flos Carthami 2-4 part, Radix Et Rhizoma Rhei 2-4 part, Rhizoma Coptidis 1-2 part, Herba Menthae 1-2 part, Radix Glycyrrhizae 1-2 part.
The preferred feedstock medicine weight proportion of making the Chinese herbal medicine infusion liquid that the present invention treats cow endometritis is: 6 parts of Herba Leonuris, 4 parts of Radix Angelicae Sinensis, 2 parts of Rhizoma Chuanxiongs, 2 parts of Radix Scutellariaes, 2 parts on Flos Carthami, 2 parts of Radix Et Rhizoma Rhei, 1 part of Rhizoma Coptidis, 2 parts of Herba Menthaes, 1 part in Radix Glycyrrhizae.
The preparation method that the present invention treats the Chinese herbal medicine infusion liquid of cow endometritis may further comprise the steps:
(1) takes by weighing Herba Leonuri, Radix Angelicae Sinensis, Rhizoma Chuanxiong, Radix Scutellariae, Flos Carthami, Radix Et Rhizoma Rhei, Rhizoma Coptidis, Herba Menthae, Radix Glycyrrhizae herbal decoction piece by weight ratio respectively, and be ground into the Chinese herbal medicine coarse powder respectively;
(2) get Radix Angelicae Sinensis, Rhizoma Chuanxiong, Radix Scutellariae, Radix Et Rhizoma Rhei, Rhizoma Coptidis, Radix Glycyrrhizae Chinese herbal medicine coarse powder, set by step 7 of (1) Chinese herbal medicine coarse powder gross weight of obtaining extraordinarily injection water soak 1-1.5h, heating decocts 25-35min, add Herba Leonuri, Flos Carthami, Herba Menthae Chinese herbal medicine coarse powder decoction 25-35 min again, incline and to boil for the first time liquid, again set by step 5 of (1) Chinese herbal medicine coarse powder gross weight of obtaining extraordinarily injection water decoct twice, decoct 25-35min at every turn, obtain boiling liquid and boiling liquid for the third time for the second time;
(3) liquid is boiled in three times of obtaining of combining step (2), filters, and is concentrated into every milliliter of injection water and contains crude drug 1g, obtains concentrating boiling liquid;
(4) the concentrated concentration of boiling 3 times of weight of liquid adding that step (3) is obtained is 95% ethanol, stirs while adding, and leaves standstill 24h, removes precipitation and reclaims ethanol, is concentrated into every milliliter of injection water and contains crude drug 2g, obtains concentrated medicament;
(5) dripping dilute sodium hydroxide makes the pH of the concentrated medicament that step (4) obtains reach 7.0, add active carbon by 0.15% of described concentrated medicament weight, boil 25-30min, place 12h, respectively add benzyl alcohol and tween 80 by 1% of described concentrated medicament weight again, stir evenly, the G-3 sintered glass funnel filters, and filtrate complements to every milliliter of injection water with injection water and contains crude drug 2g, fill, sterilization namely gets the Chinese herbal medicine infusion liquid that the present invention treats cow endometritis.
The each component pharmacological effect is in the prescription of the present invention:
The Herba Leonuri effect: Herba Leonuri is the young stem and leaf that Labiatae is planted Herba Leonuri.Nature and flavor hardship, suffering are slightly cold.Return liver, pericardium channel.Contain leonurine, stachydrine, leonuridine, leonurinine, linolenic acid, β-linolenic acid, lauric acid, oleic acid, benzoic acid, globulariacitrin, Fumaric acid, sterol, vitamin A etc., also contain arginine, 4-guanidine radicals-1-butanols, 4-guanidine radicals-butanoic acid, stachyose in addition.Herba Leonuri decoct, ethanol extractum and contained leonurine etc. all are excitation to the uterus of multiple animals such as rabbit, cat, dog, Cavia porcellus.Give the rabbit isolated uterine with the Herba Leonuri decoct, no matter unpregnancy, early pregnancy, late pregnancy or puerperal the uterus, all be excitation, to uterus in place, through intravenous injection fast, excitation appears namely behind the 30min, its intensity and action time increase with the consumption increasing.Have invigorate blood circulation, the effect of blood stasis dispelling, the water that disappears.Cure mainly the vaginal bleeding during pregnancy difficult labour, retention of placenta, abdominal pain due to blood stasis is rushed down blood, the carbuncle skin infection.
The Radix Angelicae Sinensis effect: call driedly return, horse hair Radix Angelicae Sinensis, Radix Angelicae Gigatis.Sweet in the mouth, suffering, hardship, warm in nature.Return liver, the heart, spleen channel.Angelica root contains volatile oil and involatile constituent.Neutral oil composition in the volatile oil has butylidenephthalide, nopinene, australene, camphene, to P-cymene, 1-Phenylethanone., β-bisabolence, ligustilide, normal-butyl Si Qingization Fu lactone, n-dodecane alcohol, bergapton etc.Still contain other compositions, as stigmasterol, sitosterol, stigmasterol-D-glycoside etc.In addition, also contain sucrose, fructose, glucose; Kind of inorganic elements surplus vitamin A, vitamin B12, the vitamin E, 17 seed amino acids and sodium, potassium, calcium, magnesium etc. 20.Pregnant and puerperal variously mainly is excitation in the body uterus to various preparations such as Radix Angelicae Sinensis decoct, tincture, petroleum ether immersion, infusion to unpregnancy, early pregnancy, the evening of animals such as anesthesia Canis familiaris L., cat, rabbit.Radix Angelicae Sinensis all has antibacterial action to escherichia coli, streptococcus etc.External can be accelerated wound healing, and contrafluxion, leukocyte and fiber are soaked into, and newborn epithelium regeneration has hemostasis and strengthens the tip Circulation local organization.Have enrich blood, invigorate blood circulation, pain relieving, the effect of moisturizing laxation, cure mainly all cards of blood deficiency, asthenia cold abdominalgia, the dryness of the intestine dyschezia, heavy after the bloody dysentery, ulcer sores.
Rhizoma Chuanxiong effect: Rhizoma Chuanxiong original name rhizome of chuanxiong Qiong.Acrid in the mouth, warm in nature.Return liver, gallbladder, pericardium channel.Contain alkaloid, as ligustrazine (tetramethylpyazine), gland pyrimidine, L-valyl-L-valine acid anhydride, choline.Contain volatile oil, as pentadecanoic acid ethyl ester, ethyl palmitate, heptadecanoic acid ethyl ester, different heptadecanoic acid ethyl ester etc.Contain lactone compound, as butylphthalide, butylidenephthalide, Chuan Xiong Fu lactone.Contain organic acid, as ferulic acid, folic acid, vanillic acid, protocatechuic acid.Also contain phthalide analog compound 4-hydroxyl-3-butylphthalide and phthalide derivant, as cnidium lactone, chuanxingol etc.In addition, still contain vanillin, cupreol, spathulenol, vitamin A, sucrose, fatty wet goods.Rhizoma Chuanxiong has inhibitory action to escherichia coli, Bacillus proteus, bacillus pyocyaneus etc.Rhizoma Chuanxiong water logging agent also has inhibitory action to some pathogenic dermatophytes in vitro.Blood-activating and qi-promoting is arranged, the effect of wind-expelling pain-stopping.Be used for pacifying nerve, lump in the abdomen stomachache, diseases such as breast side of body twinge.
Radix Scutellariae effect: Radix Scutellariae another name Radix Scutellariae, scutellaria tea, tsuchikane tea root etc.The property hardship, cold.Return lung, gallbladder, spleen, large intestine, small intestine meridian.Scutellariae,radix contains flavone compound: baicalin, and neobaicalein, baicalin, wogonin, wogonoside, oroxylin A, 7-methoxyl group baicalin, the dihydro oroxylin, chrysin also contains effective ingredient such as cupreol, campesterol and stigmasterol in addition.Escherichia coli, Bacillus proteus, bacillus pyocyaneus, staphylococcus, hemolytic vessel used to hold grain at the imperial sacrifice coccus etc. there is inhibitory action.Have that heat clearing and damp drying, removing heat from blood are antiabortive, the effect of detoxifcation.Cure mainly diseases such as epidemic febrile disease, damp and hot jaundice, carbuncle furuncle.
The Flos Carthami effect: Flos Carthami claims Flos Carthami again.Warm in nature, acrid in the mouth.GUIXIN, Liver Channel.Contain Carthamus yellow and carthamin, carthamin gets glucose Flos Carthami element through hydrochloric acid hydrolysis.Contain the Polyphenols composition again: chlorogenic acid, with coffee acid, catechol, Jiao's property catechol, DOPA.Also contain kind surplus the volatile ingredient 80, determined having of structure: ethyl acetate, benzene, 1-amylene-3-alcohol etc. also contain rhamnose, arabinose, xylose, glucose, mannose, nonacosane, cupreol, Palmic acid, myristic acid, lauric acid, α, γ-glycerol-1,3-dipalmitate, oleic acid, linoleic acid, cupreol-3-O-glycoside.Other contains the Flos Carthami polysaccharide, is by glucose, xylose, arabinose and galactose etc.The effect that the latus rectum of invigorating blood circulation, eliminating stasis to stop pain are arranged.Be used for diseases such as lochia is not all right, lump in the abdomen mass in the abdomen.
The Radix Et Rhizoma Rhei effect: Radix Et Rhizoma Rhei has another name called RADIX ET RHIZOMA RHEI OFFICILALIS, is being collectively referred to as of perennial plant of multiple Polygonaceae Rheum, and bitter in the mouth is cold.Return stomach, large intestine, liver, spleen channel.Gas delicate fragrance, bitter in the mouth and little puckery.The anthraquinone glycoside that contains and dianthracene ketone glycoside have the diarrhea inducing effect, and the chrysophanic acid that contains, emodin, aloe-emodin have the resisting pathogenic microbes effect, and responsive antibacterial has diseases such as staphylococcus, Hemolytic streptococcus.Also have antiinflammatory, refrigeration function in addition.Anthraquinone derivative can suppress non-specific immunity.The contained tannin of Radix Et Rhizoma Rhei has good antioxidation.Purging heat and dredging bowels, removing pathogenic heat from blood and toxic substance from the body are arranged, the effect of removing blood stasis.Be used for the excess-heat constipation, stagnant stomachache, dysentery is not well, jaundice due to damp-heat.
Rhizoma Coptidis effect: Rhizoma Coptidis another name Wang Lian, Zhi Lian.Dry rhizome for cohosh Rhizoma Coptidis, Coptis deltoidea C.Y.Cheng et Hsiao and Coptis Teeta Wall.(root) hardship, cold, nontoxic.GUIXIN, spleen, stomach, liver, gallbladder, large intestine channel.Rhizome contains multiple alkaloid, mainly is berberine, claims berberine again, is coptisine, 13-methyl-.psi.-coptisine., palmatine, jateorhizine, Columbamine secondly.Still contain obacunone, obakulactone, magnoflorine, ferulic acid etc.Leaf contains berberine.In addition, also contain various trace elements in the Rhizoma Coptidis.Have effects such as anti-staphylococcus aureus, Hemolytic streptococcus.The antagonism bacteriotoxin, reduction staphylococcus aureus coagulase, hemolysin are tired, and reduce colibacillary virulence.Heat clearing and damp drying is arranged, the effect of eliminating fire and detoxication.Be used for damp and hot feeling of fullness, dysentery, jaundice etc.
The Herba Menthae effect: Herba Menthae popular name lunar caustic grass, another name HERBA MENTHAE, water mint, Herba Menthae Rotundifoliae, people's Adiantum monochlamys Eaton. are for the labiate Herba Menthae namely belongs to other dry herbs, acrid in the mouth, cold together.Return lung, Liver Channel.Contain volatile oil, main constituent is menthol in the oil, is menthone secondly, also contains acetic acid peppermint ester, camphene, limonene, isomenthone, pinene piperitenone, resin and a small amount of tannin, rosmarinic acid.This siphonal lobe still contains multiple free amino acids such as threonine, alanine, glutamic acid, agedoite.The wind heat of dispersing is arranged, clearing throat, rash detoxifcation, dispersing the stagnated live-QI to relieve the stagnation of QI and antipruritic effect are used for diseases such as lossless, colic and scrofula.
The Radix Glycyrrhizae effect: Radix Glycyrrhizae mainly contains compositions such as glycyrrhizic acid, glycyrrhizin, 24-hydroxyl enoxolone, licoflavone, glycyrrhizin, isoliquiritigenin, Angelica Polysaccharide.Glycyrrhizic acid is natural antidote, can be used for body defying age, enhancing immunity, keeps water-electrolyte metabolism balance etc.The effect that invigorating the spleen and replenishing QI, heat-clearing and toxic substances removing, relieving spasm to stop pain, coordinating the actions of various ingredients in a prescription are arranged.Be used for weakness of the spleen and stomach, fatigue and weakness, gastral cavity abdomen, cushion toxicity, strong.
Prescription foundation of the present invention: Chinese veterinarian claims that cow endometritis is band disease, many infection because of female animal antibacterial in puerperal, virus, or owing to the pregnancy period feeding and management is not good at, conceived burden is excessive, factors such as excessively usage, childbirth cause QI and blood retardance or deficient, and then the positive deficiency of vital energy of body declines, and pathogen is taken advantage of a weak point.The Therapeutic Principle is blood circulation promoting and blood stasis dispelling normally, the circulation of qi promoting of enriching blood.Therefore, prescription should be with anti-inflammation, blood circulation promoting and blood stasis dispelling, heat-clearing and toxic substances removing, astringing to arrest bleeding, go to lose granulation promoting and enhancing human body immunity power is principle.Rhizoma Coptidis, Radix Scutellariae, Radix Et Rhizoma Rhei anti-inflammation, heat clearing and damp drying, parasite killing in the side.Herba Leonuri has promoting blood flow to regulate menstruation, the effect of the tissue regeneration promoting that gets rid of illness, and can strengthen strength of uterine contraction and contraction frequency, improves the tensile force of uterus muscle, accelerates involution of uterus process.Safflower effective ingredients is Carthamus yellow, but promoting blood circulation to restore menstrual flow, and blood stasis dispelling has stronger blood circulation promoting, adjusts the uterus function, effects such as dissipating blood stasis row is turbid, reducing swelling and alleviating pain.Radix Angelicae Sinensis can promote the macrophage secrete cytokines, strengthens immunologic function, can promote bone marrow and splenocyte hemopoietic function, significantly increases hemoglobin and RBC number.The main component ligustrazine of Rhizoma Chuanxiong, the gathering of platelet inside and outside is all had the obvious suppression effect, the whole blood high viscosity is descended, plasma viscosity, erythrocyte aggregation index, packed cell volume obviously descend, increase deformable index, hemorheological property is had good improvement effect.Herba Menthae has stronger osmosis, can promote medicine to accelerate diffusion in uterus, be evenly distributed, and to the mucosa osmotic absorption, strengthens the anti-inflammatory anti-itch effect, is conducive to give full play to the effectiveness of intrauterine medicine.With the sweet temperature QI invigorating of Radix Glycyrrhizae and middle spleen invigorating, current twelve channels and collaterals, coordinating the actions of various ingredients in a prescription.In the side all medicines mutually and, play blood circulation promoting and blood stasis dispelling, heat-clearing and toxic substances removing, anti-inflammation, the involution of uterus altogether, improve the effect of immunity of organisms and resistance against diseases.Be applicable to the cow endometritis that a variety of causes causes.Use for convenient, make infusion liquid.
The present invention treats the Chinese herbal medicine infusion liquid of cow endometritis, be on the basis of having carried out a large amount of practical applications, according to the traditional medicine ancient prescription, form in conjunction with sick of modern popular, nosetiology and immunologic theoretical screening, be used for the treatment of cow endometritis, short treating period, cost is low, cure rate is high, safely, have no side effect, easy to use.
The specific embodiment
The invention will be further described below in conjunction with embodiment.
Embodiment:
1, difference weighting raw materials (herbal decoction piece): Herba Leonuri 270 g, Radix Angelicae Sinensis 180 g, Rhizoma Chuanxiong 90 g, Radix Scutellariae 90 g, Flos Carthami 90 g, Radix Et Rhizoma Rhei 90 g, Rhizoma Coptidis 45 g, Herba Menthae 100 g, Radix Glycyrrhizae 45 g;
2, the above-mentioned raw materials medicine is ground into the Chinese herbal medicine coarse powder respectively;
3, Radix Angelicae Sinensis, Rhizoma Chuanxiong, Radix Scutellariae, Radix Et Rhizoma Rhei, Rhizoma Coptidis, Chinese medicine of licorice root coarse powder are mixed, add water 7000 mL and soak 1 h, slow fire boiling 30 min, put into Herba Leonuri, Flos Carthami, Herba Menthae Chinese herbal medicine coarse powder again, slow fire boiling 30 min obtain boiling liquid for the first time, and then add water 5000 mL decoction secondary at every turn, each 30 min that decoct obtain boiling liquid and boiling liquid for the third time for the second time;
4, merge above-mentioned three times and boil liquid, filter, be concentrated into every milliliter of injection water and contain crude drug 1g, obtain concentrating and boil liquid, add concentration then and be 95% ethanol 1500 mL, stir while adding, leave standstill 24h, go precipitation to get supernatant, boil off ethanol, be concentrated into every milliliter of injection water and contain crude drug 2g, obtain concentrated medicament;
5, dripping dilute sodium hydroxide makes the pH of above-mentioned concentrated medicament reach 7.0, the active carbon that adds this concentrated medicament weight 0.15% boils 30min, place 12h, filter, respectively add 1% benzyl alcohol and tween 80 again by this concentrated medicament weight, stir evenly, G-3 number vertical banyan glass funnel filters, and filtrate is supplied 500mL with injection water, every bottle of 10mL of fill, 100 ℃, 30 min sterilization namely get the Chinese herbal medicine infusion liquid that the present invention treats cow endometritis.
It is as follows to utilize the present invention to treat some tests that cow endometritis Chinese herbal medicine infusion liquid carries out:
1, Chinese herbal medicine infusion liquid of the present invention is to the drug sensitive test of cow endometritis The main pathogenic fungi
Choose clinical trouble endometritis milch cow case, through separating, identifying, select pathogenic stronger 2 kinds of pathogen such as staphylococcus aureus and streptococcus agalactiae.Take by weighing a certain amount of Chinese herbal medicine infusion liquid of the present invention, 4 ℃ of preservations are standby.Staphylococcus aureus, the streptococcus agalactiae of clinical separation are inoculated in flat board earlier, behind 37 ℃ of cultivation 18 h, select colonies typical and be inoculated in the 5 mL meat soups, behind 37 ℃ of cultivation 18 h, 0.5 unit's standard opacity tube in Maxwell is diluted to 105 CFU/mLs with meat soup with bacterium liquid than turbid, and is standby.
Medicament sensitivity test adopts the plate punch method, adds 1g/mL Chinese herbal medicine infusion liquid of the present invention in the hole, and overfolw hole is not advisable outward to fill it up with.Other gets penicillin 10 IU/ sheets, ampicillin 5 μ g/mL, three kinds of antibiotic drug sensitive tests of ciprofloxacin 1 μ g/mL and compares, and places 37 ℃ of constant incubators to cultivate 24 h, measures antibacterial circle diameter, the drug sensitive test triplicate of every kind of medicine.Criterion: according to the standard of " pharmacology of Chinese materia medica " and " U.S. clinical laboratory Standardization Research the relevant drug sensitive test standardization of version in 2005 ", namely bacteriostatic diameter 〉=20 mm are for extremely quick, 20 mm〉bacteriostatic diameter 〉=15 mm are Gao Min; 15 mm〉bacteriostatic diameter 〉=10 mm are quick in being, and 10 mm are following to be hypoallergenic.
Chinese herbal medicine infusion liquid medicament sensitivity test of the present invention the results are shown in Table 1.
Table 1 Chinese herbal medicine infusion liquid of the present invention is to the drug sensitive tests of two kinds of bacterium mm as a result
| Medicine | Staphylococcus aureus | Streptococcus agalactiae |
| Penicillin | 20.0±1.2 | 19.7±0.5 |
| Ampicillin | 19.3±0.5 | 19.5±1.0 |
| Ciprofloxacin | 19.5±1.0 | 20.0±1.2 |
| Chinese herbal medicine infusion liquid of the present invention | 22.5±1.5 | 21.1±1.6 |
Table 1 shows that to staphylococcus aureus, Chinese herbal medicine infusion liquid of the present invention is for extremely quick, and bacteriostatic diameter reaches 22.5 mm, and penicillin, ampicillin and ciprofloxacin are Gao Min; To streptococcus agalactiae, Chinese herbal medicine infusion liquid of the present invention is for extremely quick, and bacteriostatic diameter reaches 21.1 mm, and other medicines such as penicillin, ampicillin and ciprofloxacin are Gao Min.Drug sensitivity test shows that staphylococcus aureus and streptococcus agalactiae are the most responsive to Chinese herbal medicine infusion liquid of the present invention; Ampicillin takes second place; Chinese herbal medicine infusion liquid of the present invention is stronger to the inhibitory action of staphylococcus aureus and streptococcus agalactiae, and ampicillin takes second place.Chinese herbal medicine infusion liquid of the present invention is good than antibiotic such as penicillins to the inhibition of 2 kinds of pathogen of cow endometritis, should be as first-selection in the practice of control cow endometritis.
2. the safety testing of Chinese herbal medicine infusion liquid of the present invention
2.1 acute toxicity test
2.1.1 the method for gavaging: 20 mices, be divided into 5 groups at random, 4 every group, male and female half and half.Each test group is equivalent to crude drug in whole 1 g with 0.2 mL/20g, 0.4 mL/20g, 0.6 mL/20g, 0.8 mL/20g(1 mL respectively to mice) the filling stomach; Control group mice gavages distilled water with 0.8mL/20g.By experiment, Chinese herbal medicine infusion liquid of the present invention does not cause dead mouse for mouse stomach (1 mL is equivalent to crude drug in whole 1 g) by 0.8 mL/20g.Illustrate mice to the minimum dosis tolerata of Chinese herbal medicine infusion liquid of the present invention more than 0.8 g/20 g.Test group and matched group relatively mice change at aspect no abnormality seens such as spirit, quilt hair, behavior, appetite.
2.1.2 intraperitoneal injection: 6 mices of pilot study are divided into 3 groups, male and female half and half.The lumbar injection said preparation is obtained the maximal dose 0.2 mL/20 g that makes the mice survival according to mice survival number, obtains according to the dead mouse number to make the whole dead minimum dose 1.0 mL/2 0g(1mL of mice be equivalent to crude drug in whole 1g).30 mices of formal test are divided into 6 groups at random, 5 every group.According to the pilot study result, establish 5 dosage groups: the agent distance is than being no more than 1.5 between 10.0 g/kg, 15.0 g/kg, 22.5 g/kg, 33.8 g/kg, 50.6 g/kg(dosage groups), 1 matched group: intraperitoneal injection of saline 1.0 mL/20g.Mice performance rapid breathing after the medication, palpitating speed, exophthalmos is erect by hair, hogback, whole body is little to be trembled, and tail is crispaturaed, and sufficient pawl shrinks, low position walking, and then the postural reflex disappears, catacleisis and discharge of eye is arranged up and down, respiratory frequency slows down, and is then dead.Cut open inspection and see heart congestion, other organ no abnormality seen.By formula: Log LD50=Xm-d (∑ p-0.5), can try to achieve Chinese herbal medicine infusion liquid of the present invention is that 0.6942 g/20g(LD50 is 34710 mg/kg to the LD50 of mice).According to veterinary drug safety evaluatio standard, as LD50〉belong to actual nontoxic during 5 000 mg/kg, Chinese herbal medicine infusion liquid of the present invention is 34710 mg/kg to the LD50 of mice〉5 000 mg/kg, illustrate nontoxic.
2.2 rabbit eye irritation test: adopt the consubstantiality own control.Get 4 of healthy rabbits, check two of every rabbit, normal person can be used for test.Draw back palpebra inferior gently, behind eyeball, Chinese herbal medicine infusion liquid of the present invention and each 0.1 mL of normal saline are splashed into respectively in the right and left eyes conjunctival sac of rabbit, two amounts of splashing into equate, the about 1-2 s of eyelid that gently sleeps then in order to avoid medicinal liquid and normal saline flow out, influences result of the test.The eye conjunctiva has or not hyperemia, edema, photophobia and irritant reaction such as shed tears when observing 1 h, 6 h after the administration, 12 h, 24 h and 36 h.
By the contrast to the medication of 4 rabbit right and left eyes and normal saline, the result shows to have behind medication 1 h and slightly sheds tears and congested phenomenon that shedding tears behind 12 h stops, and congested phenomenon is more obvious, and congested phenomenon weakens gradually behind 24 h, and the congested phenomenon of 36 h disappears.Result of the test shows that the Chinese herbal medicine infusion liquid of developing of the present invention is to the mucosa nonirritant.
2.3 the detection of hematological indices before and after the medication for the treatment of by Chinese herbs cow endometritis: clinical definite is 10 of the cases (treatment of Chinese herbal medicine infusion liquid) of milch cow uterine veil inflammation, 8 of the normal healthy controls group milch cows same period (not medication treatment), 10 of ill matched group milch cows (not medication treatment).Chinese medicinal perfusion liquid treatment group milch cow use treatment by Chinese herbs making a definite diagnosis ill back, and dosage 400-500 mL waits warm uterus and pours into, and every day 1 time, 3 d were 1 course for the treatment of.Chinese herbal medicine infusion liquid treatment group milch cow is taken a blood sample once with each jugular vein of healing back before making a definite diagnosis the back medication respectively, carries out red blood cell count(RBC), numeration of leukocyte, content of hemoglobin, differential blood count etc.Healthy cow matched group and the blood sampling same period of ill milch cow matched group are carried out red blood cell count(RBC), numeration of leukocyte, content of hemoglobin, differential blood count equally.The measurement result of RBC number, hemoglobin content and packed cell volume sees Table 2 in the blood, and blood middle leukocytes sum and differential blood count the results are shown in Table 3.
The measurement result of RBC number, hemoglobin content and packed cell volume in table 2 blood
Table 3 blood middle leukocytes sum and differential blood count result
As can be seen from Table 2, puerperal, cow blood RBC number, content of hemoglobin, the packed cell volume of health maintained normal level substantially, RBC number, content of hemoglobin, the packed cell volume of suffering from the endometritis milch cow are less, RBC number, content of hemoglobin, packed cell volume that Chinese herbal medicine infusion liquid treatment group milch cow of the present invention is cured the back milch cow in medication are kept normal level, illustrate that Chinese herbal medicine infusion liquid of the present invention can increase the erythrocyte of milch cow, content of hemoglobin, packed cell volume quantity.Erythrocyte and hemoglobin play an important role in body hemopoietic and oxygen transportation, and be very important to animal survival, growth and production, and erythrocyte also has the partial immunity function.After the endometritis of Chinese herbal medicine infusion liquid treatment milch cow of the present invention, these three indexs significantly increase, reach normal value, illustrating that Chinese herbal medicine infusion liquid of the present invention has the RBC number that increases in the blood and the effect of content of hemoglobin, may be that the hematonic Radix Angelicae Sinensis in the Chinese herbal medicine infusion liquid of the present invention has been brought into play main effect.
As can be seen from Table 3, suffer from the total white blood cells in the endometritis cow blood, the neutrophilic granulocyte number, mononuclear cell is significantly higher than the healthy cow same period, after Chinese herbal medicine infusion liquid treatment of the present invention, total white blood cells, the neutrophilic granulocyte number, mononuclear cell significantly descends, and without the total white blood cells of the ill milch cow of Chinese herbal medicine infusion liquid of the present invention treatment, the neutrophilic granulocyte number, mononuclear cell maintains higher level all the time, be higher than the normal healthy controls group, illustrate that Chinese herbal medicine infusion liquid of the present invention can reduce the total white blood cells of suffering from the endometritis cow blood, the neutrophilic granulocyte number, mononuclear cell makes it to recover normal level; Suffer from the percentage of lymphocyte of endometritis milch cow than the same period healthy cow on the low side, after Chinese herbal medicine infusion liquid treatment of the present invention, this ratio fast rise, milch cow after the healing is compared this index difference with healthy cow not remarkable, shows that Chinese herbal medicine infusion liquid of the present invention can improve the percentage of lymphocyte of suffering from the endometritis cow blood leukocyte.Leukocyte is body
Important immunocyte has only when body is in and infects defence during the phase, and this cell just can be bred in a large number, much more neutrophilic granulocyte sees that at this moment, this cell is discharged into peripheral blood from the hemopoietic organ in the suppurative infection phase, and from microcirculatory vascular, disengage, soak in areas of inflammation.This cell has active motor capacity and phagocytic function; but the life-span is shorter; become pus cell after the death, this also is one of endometritis reason of often being converted into chronic purulence endometritis, and neutrophilic granulocyte increases the evolution of also having pointed out local inflammation in the milch cow body.In the test, the neutrophilic granulocyte of Chinese herbal medicine infusion liquid treatment group milch cow of the present invention significantly descends, and is just reflecting that also Chinese herbal medicine infusion liquid of the present invention brought into play effect preferably, has eliminated the proinflammatory factors in the body, just makes the ratio of neutrophilic granulocyte return to normal level.Lymphocyte also is important immunologically competent cell, do not possess phagocytic function, the main antibody materials that produces in the body, when viral infection takes place when, quantity significantly rises in the body, when Abwehrkraft des Koepers decline or immunologic hypofunction, the ratio regular meeting of this cell descends, the percentage of lymphocyte of suffering from the endometritis milch cow in this test significantly descends, also reflect these milch cows because the hypoimmunity of self, cause a large amount of breedings of some conditioned pathogens of intrauterine and cause cow endometritis, Chinese herbal medicine infusion liquid of the present invention has been regulated the whole defensive enginery of animal body just, the autoimmunity of ill milch cow is improved, and lymphocytic ratio just rises to normal level.Under the normal condition, mononuclear cell exists in the middle of the blood, but negligible amounts.When the body part was inflamed, because inflammatory stimulus, this cell was bred in a large number, by blood circulation, arrived areas of inflammation, the performance phagocytic function, and it often comes across the inflammation later stage, chronic inflammatory disease process etc.This result of the test shows that Chinese herbal medicine infusion liquid of the present invention can make the mononuclear cell ratio increase, and makes the sx of endometritis, illustrates that body repairs the endometrium that inflammation is destroyed.
Above experimental result shows, characteristics such as endometritis Chinese herbal medicine infusion liquid medical instrument of the present invention has anti-inflammation, adjust the uterus function, improve local blood circulation, adjust intrauterine environment, increase that medicine spreads in uterus, infiltration and Absorption, nonirritant, raising immunity.
Claims (3)
1. a Chinese herbal medicine infusion liquid for the treatment of cow endometritis is characterized in that it is to be made by following raw materials by weight proportions: Herba Leonuri 5-7 part, Radix Angelicae Sinensis 4-6 part, Rhizoma Chuanxiong 2-4 part, Radix Scutellariae 2-4 part, Flos Carthami 2-4 part, Radix Et Rhizoma Rhei 2-4 part, Rhizoma Coptidis 1-2 part, Herba Menthae 1-2 part, Radix Glycyrrhizae 1-2 part.
2. the Chinese herbal medicine infusion liquid for the treatment of cow endometritis according to claim 1 is characterized in that it is to be made by following raw materials by weight proportions: 6 parts of Herba Leonuris, 4 parts of Radix Angelicae Sinensis, 2 parts of Rhizoma Chuanxiongs, 2 parts of Radix Scutellariaes, 2 parts on Flos Carthami, 2 parts of Radix Et Rhizoma Rhei, 1 part of Rhizoma Coptidis, 2 parts of Herba Menthaes, 1 part in Radix Glycyrrhizae.
3. preparation method for the treatment of the Chinese herbal medicine infusion liquid of cow endometritis is characterized in that it may further comprise the steps:
(1) takes by weighing Herba Leonuri, Radix Angelicae Sinensis, Rhizoma Chuanxiong, Radix Scutellariae, Flos Carthami, Radix Et Rhizoma Rhei, Rhizoma Coptidis, Herba Menthae, Radix Glycyrrhizae herbal decoction piece by weight ratio respectively, and be ground into the Chinese herbal medicine coarse powder respectively;
(2) get Radix Angelicae Sinensis, Rhizoma Chuanxiong, Radix Scutellariae, Radix Et Rhizoma Rhei, Rhizoma Coptidis, Radix Glycyrrhizae Chinese herbal medicine coarse powder, set by step 7 of (1) Chinese herbal medicine coarse powder gross weight of obtaining extraordinarily injection water soak 1-1.5h, heating decocts 25-35min, add Herba Leonuri, Flos Carthami, Herba Menthae Chinese herbal medicine coarse powder decoction 25-35 min again, incline and to boil for the first time liquid, again set by step 5 of (1) Chinese herbal medicine coarse powder gross weight of obtaining extraordinarily injection water decoct twice, decoct 25-35min at every turn, obtain boiling liquid and boiling liquid for the third time for the second time;
(3) liquid is boiled in three times of obtaining of combining step (2), filters, and is concentrated into every milliliter of injection water and contains crude drug 1g, obtains concentrating boiling liquid;
(4) the concentrated concentration of boiling 3 times of weight of liquid adding that step (3) is obtained is 95% ethanol, stirs while adding, and leaves standstill 24h, removes precipitation and reclaims ethanol, is concentrated into every milliliter of injection water and contains crude drug 2g, obtains concentrated medicament;
(5) dripping dilute sodium hydroxide makes the pH of the concentrated medicament that step (4) obtains reach 7.0, add active carbon by 0.15% of described concentrated medicament weight, boil 25-30min, place 12h, respectively add benzyl alcohol and tween 80 by 1% of described concentrated medicament weight again, stir evenly, the G-3 sintered glass funnel filters, and filtrate complements to every milliliter of injection water with injection water and contains crude drug 2g, fill, sterilization namely gets the Chinese herbal medicine infusion liquid that the present invention treats cow endometritis.
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| CN110917260A (en) * | 2019-12-27 | 2020-03-27 | 甘肃农业大学 | Traditional Chinese medicine composition for preventing sheep endometritis, preparation method and application |
| CN111521694A (en) * | 2020-04-10 | 2020-08-11 | 中国水产科学研究院长江水产研究所 | Application of ligustilide in eliminating quinolone drug residues in cultured fishes |
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Application publication date: 20130703 |