CN105749099A - Probiotic fermented traditional Chinese medicine composition for treating gastric cancer and preparation method and application thereof - Google Patents
Probiotic fermented traditional Chinese medicine composition for treating gastric cancer and preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a probiotic fermented traditional Chinese medicine composition for treating a gastric cancer and a preparation method and application thereof.The fermented traditional Chinese medicine composition is prepared through the steps that extraction and enzymolysis are performed on radix panacis quinquefolii, radix bupleuri, fructus aurantii, radix scutellariae, rhizoma pinelliae, herba salviae chinensis, cuttle bones, bulbus fritillariae thunbergii, radix paeoniae alba, rhizoma chuanxiong, rhizoma cyperi processed with vinegar, rhizoma galangae, radix angelica sinensis, herba cistanche and prepared liquorice roots, fructose, peptone, yeast cream, yeast powder, inorganic salt, a sweetening agent and the like are added, probiotic fermentation is performed, and then dietary fiber and prebiotics are added.The probiotic fermented traditional Chinese medicine composition has the effects of inhibiting gastric cancer cell growth, improving the immunity, regulating the intestinal flora balance, improving the gastrointestinal function and the like and is capable of rapidly achieving the effects and good in mouthfeel and treatment effect.Experiments show that the fermented composition can significantly increase the quantity of mouse T lymphocytes and improve the organism immunity, the inhibition ratio on gastric cancer cells can reach 65%, and therefore the fermented composition is good in treatment effect on the gastric cancer.
Description
Technical field
The present invention relates to a kind of probiotics fermention Chinese medicine composition treating gastric cancer and preparation method thereof, belong to technical field of Chinese medicine.
Background technology
Gastric cancer is one of modal malignant tumor in the world, the first place of all kinds of tumor is occupied at its sickness rate of China, there are about 170,000 people every year and die from gastric cancer, almost close to the 1/4 of whole mortality of malignant tumors numbers, and annual also having more than 20,000 new Patients with Gastric Cancer to generate, gastric cancer is strictly the disease of a kind of serious threat people's health.Gastric cancer can betide any age, but common with 40～60 years old, and man, more than female, is about 2:1.Gastric cancer can betide any position of stomach, but is more common in gastric antrum portion, especially lesser gastric curvature side.Untreated person's average life is about 13 months.
Excision is still the only scheme effecting a radical cure early gastric cancer at present, is also the Main Means for the treatment of gastric cancer.For a long time, because it is found that gastric cancer is later, great majority belong to late tumor, and operative effect is not good enough, and within postoperative 5 years, survival rate maintains about 30% always, therefore, it is necessary to seek more more effective therapeutic scheme, to improve survival rate.
Gastric cancer treatment by Chinese herbs is a big characteristic of China's curing gastric cancer, and along with deepening continuously of gastric cancer research controlled by Chinese medicine by China in recent years, treatment by Chinese herbs is made to play more and more important effect in the treatment of gastric cancer, treatment by Chinese herbs gastric cancer is from entirety, gastric cancer treatment by Chinese herbs is not only able to be individually used for the treatment of gastric cancer, but also can with Surgery for Gastric Carcinoma, radiotherapy, the treatment means use in conjunction such as chemotherapy, comprehensive therapeutic effect can be significantly improved, especially the chemicotherapy toxic and side effects to body is being alleviated, the immunity improving patient and the effect improving the aspects such as its quality of life are obvious, can effectively prevent the recurrence of tumor, transfer.
nullAt present，The Patents of domestic compound of Chinese medicine gastric cancer is existing a lot，Such as the Yiqihuoxue Decoction Chinese medicine preparation preventing and treating gastric cancer and preparation method thereof-CN201310543123、The Chinese medicine preparation for the treatment of gastric cancer and preparation method thereof-CN200710052851、A kind of Chinese medicine composition-CN201110262095 treating gastric cancer、A kind of Chinese medicine composition-CN201210416744 treating gastric cancer、A kind of Chinese herbal oral decoction-CN201010003136 treating gastric cancer、A kind of Chinese medicine composition treating gastric cancer and preparation method thereof-CN201310017007、A kind of Chinese medicine treating gastric cancer and preparation method thereof-CN201210582510 etc.，Foregoing invention great majority are classical prescription、Proved recipe，Manufacture method is simple、Extensive，Such as decoct、Pill、Powder、Capsule etc.，Though there being certain curative effect，But raw material availability is low，Mouthfeel extreme difference，Take effect slow，Great misery is brought to patient during long-term taking.
Summary of the invention
Gastric cancer belongs to digestive system disease, and digestive tract environment is caused expensive damage because of disease itself and various chemicotherapy by patient, has especially upset the ecological balance of intestinal beneficial flora.Living away from home in normal human's intestinal substantial amounts of microbial population, wherein health is played vital effect by probiotic bacteria.Probiotic bacteria can catabolism upper digestive tract system can not catabolic composition of food, produce short-chain fatty acid, for gastrointestinal mucosa cells with nutrient material.Some composition simultaneously for oral drugs especially natural drug can carry out catabolism, the precursor substance of medicine is transformed into active pharmaceutical ingredient and plays a role.Therefore, if being aided with regulating intestinal canal flora while treatment disease, great benefit can be brought for patient undoubtedly.
The present invention solves the deficiency of existing Chinese medicinal formulae, first the clinical treatment good Chinese prescription of gastric cancer effect is carried out high efficiency extraction, utilize modern biotechnology, by Chinese medicine extraction liquid through probiotics fermention, in-vitro simulated human body intestinal canal environment, in advance to the various effective ingredient in Chinese medicine extraction liquid, decompose, directly absorbed after making it into human body, alleviate human body burden, it is equipped with dietary fiber and prebiotics simultaneously, regulate human body intestinal canal environment, develop raw material availability height, effect is strong, the Chinese medicine composition that mouthfeel is good and added value is high, meet the demand of patient, wide market.
It is an object of the present invention to provide a kind of probiotics fermention Chinese medicine composition treating gastric cancer, described Chinese medicine composition is made up of the medical material of following weight portion: Radix Panacis Quinquefolii 3 ~ 6 parts, Radix Bupleuri 3 ~ 10 parts, Fructus Aurantii 3 ~ 10 parts, Radix Scutellariae 3 ~ 10 parts, the Rhizoma Pinelliae 3 ~ 9 parts, Herba Salviae Chinensis 5 ~ 20 parts, 5 ~ 10 parts of the Piao sheath in sea, Bulbus Fritillariae Thunbergii 2 ~ 8 parts, the Radix Paeoniae Alba 6 ~ 15 parts, Rhizoma Chuanxiong 3 ~ 10 parts, Rhizoma Cyperi (processed with vinegar) 6 ~ 10 parts, Rhizoma Alpiniae Officinarum 3 ~ 6 parts, Radix Angelicae Sinensis 6 ~ 12 parts, Herba Cistanches 6 ~ 10 parts, Radix Glycyrrhizae Preparata 2 ~ 10 parts；Its preparation method is as follows:
(1) medical material processes:
A. Radix Scutellariae, Herba Salviae Chinensis add 8 ~ water of 15 times of medical material weight together, soak 20 ~ 60 minutes, carry 30 ~ 60 minutes through 115 ~ 121 DEG C of water under high pressures, filter, filtrate sterilizing, for aseptic Radix Scutellariae, Herba Salviae Chinensis mixed liquor, standby；
B. Radix Angelicae Sinensis adds 8 ~ water of 15 times of medical material weight, distills after soaking 20 ~ 60 minutes, collects the aqueous solution after distillate and distillation respectively, and distillate seals, 115 ~ 121 DEG C of sterilizings 30 ~ 60 minutes, obtains aseptic distillate, standby；
C. by adding 8 together with 12 kinds of medical materials of Radix Angelicae Sinensis medicinal residues and all the other ~ water of 15 times of medical material weight, soak 20 ~ 60 minutes, carry 30 ~ 60 minutes through 115 ~ 121 DEG C of water under high pressures, filter, filtrate merges with the aqueous solution after Radix Angelicae Sinensis distillation, obtains herbal extract；
(2) enzymolysis: by step (1) the C herbal extract obtained, maintain 70 ~ 95 DEG C of constant temperature, regulate pH to 6.0 ~ 7.0, increase temperature amylase, enzyme digestion reaction 10 ~ 15 minutes, with iodine liquid for indicator, medicinal liquid meet iodine liquid constant blue time, enzymolysis terminates, and heats to 100 DEG C of enzyme denaturing 10 ~ 20 minutes, filtering, filtrate is standby；
(3) concentration, dispensing: after the filtrate reduced in volume obtain step (2), add fructose, peptone, yeast extract, yeast powder, adds inorganic salt, adds sweeting agent, regulates pH value to 6.5 ~ 7.5, and 115 ~ 121 DEG C of sterilizings 30 ~ 60 minutes obtain sterile liquid medicine；
(4) inoculation, fermentation: when the sterile liquid medicine temperature of step (3) is down to 25 ~ 35 DEG C, the aseptic distillate of (1) B identical temperature obtained is mixed with, inoculate the Leuconostoc mesenteroides bacterium solution that pre-incubated percent by volume is 1% ~ 5%, 25 ~ 35 DEG C of cultivations, when pH value is down to 5.5 ~ 6.5, add glucose, high temperature sterilize, treat that temperature is down to 35 ~ 40 DEG C, inoculate a kind of bacillus bifidus bacterium solution that pre-incubated percent by volume is 1% ~ 5%, cultivate 6 ~ 8 hours for 37 DEG C, again inoculate a kind of lactobacillus bacterium solution that pre-incubated percent by volume is 1% ~ 5%, continue fermentation 20 ~ 24 hours；When pH value is down to 4.0 ~ 4.1, reduce fermentation temperature to 20 ~ 30 DEG C, continue fermentation to pH value and be down to 3.5 ~ 3.8, terminate fermentation, obtain Chinese medicine composition fermentation liquid；
(5) allotment, inactivation: in the Chinese medicine composition fermentation liquid that (4) obtain, add aseptic Radix Scutellariae, Herba Salviae Chinensis mixed liquor that aseptic dietary fiber, prebiotics and step (1) A extract, mixing, 115 ~ 121 DEG C of sterilizings 5 ~ 50 minutes, the probiotics fermention Chinese medicine composition of the gastric cancer that namely obtains medical treatment.
Leuconostoc mesenteroides and tunning thereof can significantly improve animal body GSH-px, SOD activity, reduce serum MDA level, and maintain the long period, there is the effect improving animal body oxidation resistance, simultaneously, the growth of normal intestinal flora can be stimulated, the common pathogens such as Shigella, Salmonella, staphylococcus aureus are had antagonism.
Preferably, described Chinese medicine composition is made up of the medical material of following weight portion: Radix Panacis Quinquefolii 3 parts, Radix Bupleuri 6 parts, Fructus Aurantii 6 parts, Radix Scutellariae 6 parts, the Rhizoma Pinelliae 5 parts, Herba Salviae Chinensis 12 parts, 10 parts of the Piao sheath in sea, Bulbus Fritillariae Thunbergii 4 parts, the Radix Paeoniae Alba 9 parts, Rhizoma Chuanxiong 5 parts, Rhizoma Cyperi (processed with vinegar) 6 parts, Rhizoma Alpiniae Officinarum 4 parts, Radix Angelicae Sinensis 9 parts, Herba Cistanches 10 parts, Radix Glycyrrhizae Preparata 2 parts；
Described amylase is α-alpha-amylase, and its Dosage calculation method is: medical material amount × 30/ enzyme unit alive, is first mixed by enzyme with a small amount of water with front.
The consumption of described fructose is weight g/ volume ml percentage ratio 0.1 ~ 1.0%, and the consumption of described glucose is weight g/ volume ml percentage ratio is 0.1 ~ 1.0%.
The consumption of described peptone be weight g/ volume ml percentage ratio 0.05 ~ 1.5%, yeast extract consumption be weight g/ volume ml percentage ratio 0.01 ~ 0.5%, yeast powder consumption be weight g/ volume ml percentage ratio 0.01 ~ 0.5%.
Described sweeting agent is the sucralose of the sucrose of weight g/ volume ml percentage ratio 3% and weight g/ volume ml percentage ratio 0.007%.
Described inorganic salt is K2HPO43H2O0.1g/100ml、KH2PO40.1g/100ml、FeSO47H2O5.0mg/100ml、ZnSO47H2O4.4mg/100ml、MgSO47H2O25mg/100ml。
Described dietary fiber is the polydextrose of weight g/ volume ml percentage ratio 1 ~ 5%.
Described prebiotics is oligofructose slurry (G55L) of weight g/ volume ml percentage ratio 0.1 ~ 0.7%.
Described Leuconostoc mesenteroides is selected from Leuconostoc mesenteroides dextran subspecies or leuconostoc mesenteroides sub species cremoris；Described bacillus bifidus is selected from bifidobacterium bifidum, bifidobacterium breve, bifidobacterium adolescentis or bifidobacteria infantis；Described lactobacillus is selected from bacillus acidophilus, Deshi Lactobacillus, lactobacillus casei, lactobacillus rhamnosus, Lactobacillus reuteri or Lactobacillus plantarum.
It is also another object of the present invention to provide the probiotics fermention Chinese medicine composition of described treatment gastric cancer suppress Growth of Gastric in preparation, improve immunity, regulating intestinal canal colony balance, the application that improves in the medicine of gastrointestinal function.Can according to known conventional method, probiotics fermention Chinese medicine composition by described treatment gastric cancer, add one or more and have identical, similar or different biological is active, and to the existing auxiliary of primary activity component list or synergism, but mutually not other naturally occurring or synthetic function compositions of antagonism or its mixture again each other, makes the mixture, Emulsion, pill, powder, capsule, tablet or the granule that are suitable to be administered orally.
The invention have the advantages that
1, this probiotics fermention Chinese medicine composition has instant effect, mouthfeel is good, suppress Growth of Gastric, improve immunity, regulating intestinal canal colony balance, improves the effects such as gastrointestinal function.
2, when the present invention is to traditional Chinese medicine extyaction, the characteristic according to medical material, utilize different technique to extract, improve the utilization rate of medical material.
3, the present invention simulates human body intestinal canal environment, first with zymotechnic, Chinese medicine extraction liquid is processed, using it as culture medium, many probiotics are utilized to carry out batch fermentation, the effective ingredient making Chinese crude drug becomes feasible to by the active substance of intestinal absorption, instant effect, good effect, decrease human body intestinal canal burden, compensate for existing tcm product take effect slowly, the deficiency of cycle length.
4, adding the water soluble dietary fiber polydextrose not being absorbed by the body and prebiotic, fructooligosaccharide in this compositions, sweet mouthfeel is agreeable to the taste, in stable condition, can significantly improve human body intestinal canal environment, improves the quality of life of patient.
5, this compositions is not added with any preservative, through high temperature sterilize, sterile filling, long shelf-life, drinks conveniently.
Detailed description of the invention
Below by way of the description of detailed description of the invention, the invention will be further described, but this is not limitation of the present invention, those skilled in the art's basic thought according to the present invention, various modifications may be made or improves, but without departing from the basic thought of the present invention, all within the scope of the present invention；Material described in embodiment, all meets respective country standard.
Embodiment 1: the preparation of the probiotics fermention Chinese medicine composition A for the treatment of gastric cancer
It is made up of the medical material of following weight portion: Radix Panacis Quinquefolii 3 parts, Radix Bupleuri 4 parts, Fructus Aurantii 4 parts, Radix Scutellariae 4 parts, the Rhizoma Pinelliae 4 parts, Herba Salviae Chinensis 8 parts, 10 parts of the Piao sheath in sea, Bulbus Fritillariae Thunbergii 4 parts, the Radix Paeoniae Alba 8 parts, Rhizoma Chuanxiong 4 parts, Rhizoma Cyperi (processed with vinegar) 4 parts, Rhizoma Alpiniae Officinarum 3 parts, Radix Angelicae Sinensis 7 parts, Herba Cistanches 7 parts, Radix Glycyrrhizae Preparata 2 parts, concrete preparation method is as follows:
(1) medical material processes: Radix Scutellariae, Herba Salviae Chinensis add the water of 8 times of medical material amounts together, soak 30 minutes, carry 60 minutes through 115 DEG C of water under high pressures, filters, and filtrate 115 DEG C of sterilizings sterilizing in 60 minutes is standby, for aseptic Radix Scutellariae, Herba Salviae Chinensis mixed liquor；Radix Angelicae Sinensis adds the water of 8 times of medical material amounts, distills after soaking 30 minutes, collects the aqueous solution after distillate and distillation respectively standby, and distillate seals, 115 DEG C of sterilizings 60 minutes, obtains aseptic distillate；Adding the water of 8 times of medical material amounts together with Radix Angelicae Sinensis medicinal residues and 12 kinds of medical materials of residue, soaks 30 minutes, carry 60 minutes through 115 DEG C of water under high pressures, filter, filtrate merges with water under high pressure extracting solution with the aqueous solution after Radix Angelicae Sinensis distillation, obtains herbal extract.
(2) enzymolysis: by (1) Chinese crude drug extracting solution, maintains constant temperature 85 DEG C, regulates pH to 6.5, increases temperature amylase, enzyme digestion reaction 10 ~ 15 minutes, with iodine liquid for indicator, medicinal liquid meet iodine liquid constant blue time, enzymolysis terminates, heating to 100 DEG C of enzyme denaturing 10 minutes, filter, filtrate is standby.
(3) concentration, dispensing: by filtrate reduced in volume in (2) after, interpolation fructose 0.6%(weight g/ volume ml), peptone 0.3%(weight g/ volume ml), yeast extract 0.1%(weight g/ volume ml), yeast powder 0.06%(weight g/ volume ml), inorganic salt (K2HPO43H2O0.1g/100ml、KH2PO40.1g/100ml、FeSO47H2O5.0mg/100ml、ZnSO47H2O4.4mg/100ml、MgSO47H2O25mg/100ml), sucrose 3.0%(weight g/ volume ml) and sucralose 0.007%(weight g/ volume ml).Regulate pH value to 7.0,115 DEG C of sterilizings 60 minutes, obtain sterile liquid medicine.
(4) inoculation, fermentation: when sterile liquid medicine temperature is down to 30 DEG C, the aseptic distillate of temperature identical in (1) is mixed with, inoculate Leuconostoc mesenteroides dextran subspecies (CICC22179) bacterium solution that pre-incubated percent by volume is 2%, 30 DEG C of cultivations, when pH value is down to 5.5, add glucose 0.6%(weight g/ volume ml), 115 DEG C of sterilizings 30 minutes, treat that temperature is down to 38 DEG C, inoculate bifidobacterium breve (AS1.2213) bacterium solution that pre-incubated percent by volume is 1%, cultivate 6 hours for 37 DEG C, again inoculate Lactobacillus plantarum (AS1.19) bacterium solution that pre-incubated percent by volume is 1%, continue fermentation 20 hours.When pH value is down to 4.0, reduce fermentation temperature to 25 DEG C, continue fermentation to pH value and be down to 3.8, terminate fermentation, obtain Chinese medicine composition fermentation liquid.
(5) allotment, inactivation: add the polydextrose 4%(weight g/ volume ml of in advance sterilizing to the Chinese medicine composition fermentation liquid in (4)), oligofructose slurry (G55L) 0.6%(weight g/ volume ml) and (1) in extract aseptic Radix Scutellariae, Herba Salviae Chinensis mixed liquor, mixing, 115 DEG C of sterilizings 20 minutes, the probiotics fermention Chinese medicine composition of gastric cancer, fill under aseptic condition (specification is 100ml/ bottle) must be treated.
Embodiment 2: the preparation of the probiotics fermention Chinese medicine composition B for the treatment of gastric cancer
It is made up of the medical material of following weight portion: Radix Panacis Quinquefolii 3 parts, Radix Bupleuri 7 parts, Fructus Aurantii 7 parts, Radix Scutellariae 4 parts, the Rhizoma Pinelliae 6 parts, Herba Salviae Chinensis 15 parts, 12 parts of the Piao sheath in sea, Bulbus Fritillariae Thunbergii 4 parts, the Radix Paeoniae Alba 9 parts, Rhizoma Chuanxiong 5 parts, Rhizoma Cyperi (processed with vinegar) 6 parts, Rhizoma Alpiniae Officinarum 5 parts, Radix Angelicae Sinensis 6 parts, Herba Cistanches 8 parts, Radix Glycyrrhizae Preparata 2 parts, concrete preparation method is as follows:
(1) medical material processes: Radix Scutellariae, Herba Salviae Chinensis add the water of 10 times of medical material amounts together, soak 60 minutes, carry 50 minutes through 115 DEG C of water under high pressures, filters, and filtrate 121 DEG C of sterilizings sterilizing in 30 minutes is standby, for aseptic Radix Scutellariae, Herba Salviae Chinensis mixed liquor；Radix Angelicae Sinensis adds the water of 10 times of medical material amounts, distills after soaking 60 minutes, collects the aqueous solution after distillate and distillation respectively standby, and distillate seals, 121 DEG C of sterilizings 30 minutes, obtains aseptic distillate；Adding the water of 10 times of medical material amounts together with Radix Angelicae Sinensis medicinal residues and 12 kinds of medical materials of residue, soaks 60 minutes, carry 50 minutes through 115 DEG C of water under high pressures, filter, filtrate merges with the aqueous solution after Radix Angelicae Sinensis distillation, obtains herbal extract.
(2) enzymolysis: by (1) Chinese crude drug extracting solution, maintains constant temperature 85 DEG C, regulates pH to 6.0, increases temperature amylase, enzyme digestion reaction 10 ~ 15 minutes, with iodine liquid for indicator, medicinal liquid meet iodine liquid constant blue time, enzymolysis terminates, heating to 100 DEG C of enzyme denaturing 15 minutes, filter, filtrate is standby.
(3) concentration, dispensing: by filtrate reduced in volume in (2) after, interpolation fructose 0.5%(weight g/ volume ml), peptone 0.2%(weight g/ volume ml), yeast extract 0.1%(weight g/ volume ml), yeast powder 0.06%(weight g/ volume ml), inorganic salt (K2HPO43H2O0.1g/100ml、KH2PO40.1g/100ml、FeSO47H2O5.0mg/100ml、ZnSO47H2O4.4mg/100ml、MgSO47H2O25mg/100ml), sucrose 3.0%(weight g/ volume ml) and sucralose 0.007%(weight g/ volume ml).Regulate pH value to 7.2,121 DEG C of sterilizings 30 minutes, obtain sterile liquid medicine.
(4) inoculation, fermentation: when sterile liquid medicine temperature is down to 28 DEG C, the aseptic distillate of temperature identical in (1) is mixed with, inoculate Leuconostoc mesenteroides dextran subspecies (CICC22179) bacterium solution that pre-incubated percent by volume is 4%, 28 DEG C of cultivations, when pH value is down to 5.5, add glucose, 115 DEG C of sterilizings 30 minutes, treat that temperature is down to 37 DEG C, (normal human separates to inoculate the bifidobacterium bifidum that pre-incubated percent by volume is 1%, identify through Institute of Microorganism, Academia Sinica) bacterium solution, cultivate 8 hours for 37 DEG C, again inoculate Deshi Lactobacillus (AS1.1480) bacterium solution that pre-incubated percent by volume is 1%, continue fermentation 24 hours.When pH value is down to 4.0, reduce fermentation temperature to 28 DEG C, continue fermentation to pH value and be down to 3.7, terminate fermentation, obtain Chinese medicine composition fermentation liquid.
(5) allotment, inactivation: add the polydextrose 5%(weight g/ volume ml of in advance sterilizing to the Chinese medicine composition fermentation liquid in (4)), oligofructose slurry (G55L) 0.5%(weight g/ volume ml) and (1) in extract aseptic Radix Scutellariae, Herba Salviae Chinensis mixed liquor, mixing, 115 DEG C of sterilizings 15 minutes, the probiotics fermention Chinese medicine composition of gastric cancer, fill under aseptic condition (specification is 100ml/ bottle) must be treated.
Embodiment 3: the preparation of the probiotics fermention Chinese medicine composition C for the treatment of gastric cancer
It is made up of the medical material of following weight portion: Radix Panacis Quinquefolii 3 parts, Radix Bupleuri 6 parts, Fructus Aurantii 6 parts, Radix Scutellariae 6 parts, the Rhizoma Pinelliae 5 parts, Herba Salviae Chinensis 12 parts, 10 parts of the Piao sheath in sea, Bulbus Fritillariae Thunbergii 4 parts, the Radix Paeoniae Alba 9 parts, Rhizoma Chuanxiong 5 parts, Rhizoma Cyperi (processed with vinegar) 6 parts, Rhizoma Alpiniae Officinarum 4 parts, Radix Angelicae Sinensis 9 parts, Herba Cistanches 10 parts, Radix Glycyrrhizae Preparata 2 parts, concrete preparation method is as follows:
(1) medical material processes: Radix Scutellariae, Herba Salviae Chinensis add the water of 12 times of medical material amounts together, soak 60 minutes, carry 60 minutes through 115 DEG C of water under high pressures, filtration, and 115 DEG C of sterilizings of filtrate 50 minutes are standby, for aseptic Radix Scutellariae, Herba Salviae Chinensis mixed liquor；Radix Angelicae Sinensis adds the water of 12 times of medical material amounts, distills after soaking 60 minutes, collects the aqueous solution after distillate and distillation respectively standby, and distillate seals, 115 DEG C of sterilizings 50 minutes, obtains aseptic distillate；Adding the water of 12 times of medical material amounts together with Radix Angelicae Sinensis medicinal residues and 12 kinds of medical materials of residue, soaks 60 minutes, carry 60 minutes through 115 DEG C of water under high pressures, filter, filtrate merges with the aqueous solution after Radix Angelicae Sinensis distillation, obtains herbal extract.
(2) enzymolysis: by (1) Chinese crude drug extracting solution, maintains constant temperature 85 DEG C, regulates pH to 6.0, increases temperature amylase, enzyme digestion reaction 10 ~ 15 minutes, with iodine liquid for indicator, medicinal liquid meet iodine liquid constant blue time, enzymolysis terminates, heating to 100 DEG C of enzyme denaturing 10 minutes, filter, filtrate is standby.
(3) concentration, dispensing: by filtrate reduced in volume in (2) after, interpolation fructose 0.4%(weight g/ volume ml), peptone 0.3%(weight g/ volume ml), yeast extract 0.08%(weight g/ volume ml), yeast powder 0.04%(weight g/ volume ml), inorganic salt (K2HPO43H2O0.1g/100ml、KH2PO40.1g/100ml、FeSO47H2O5.0mg/100ml、ZnSO47H2O4.4mg/100ml、MgSO47H2O25mg/100ml), sucrose 3.0%(weight g/ volume ml) and sucralose 0.007%(weight g/ volume ml).Regulate pH value to 7.5,115 DEG C of sterilizings 50 minutes, obtain sterile liquid medicine.
(4) inoculation, fermentation: when sterile liquid medicine temperature is down to 30 DEG C, the aseptic distillate of temperature identical in (1) is mixed with, inoculate leuconostoc mesenteroides sub species cremoris (CICC22181) bacterium solution that pre-incubated percent by volume is 3%, 30 DEG C of cultivations, when pH value is down to 5.8, add glucose 0.4%(weight g/ volume ml), 115 DEG C of sterilizings 30 minutes, treat that temperature is down to 37 DEG C, inoculate bifidobacterium adolescentis (AS1.2190) bacterium solution that pre-incubated percent by volume is 1%, cultivate 7 hours for 37 DEG C, again inoculate bacillus acidophilus (AS1.1854) bacterium solution that pre-incubated percent by volume is 1%, continue fermentation 22 hours.When pH value is down to 4.1, reduce fermentation temperature to 25 DEG C, continue fermentation to pH value and be down to 3.8, terminate fermentation, obtain Chinese medicine composition fermentation liquid.
(5) allotment, inactivation: add the polydextrose 4%(weight g/ volume ml of in advance sterilizing to the Chinese medicine composition fermentation liquid in (4)), oligofructose slurry (G55L) 0.5%(weight g/ volume ml) and (1) in extract aseptic Radix Scutellariae, Herba Salviae Chinensis mixed liquor, mixing, 115 DEG C of sterilizings 15 minutes, the probiotics fermention Chinese medicine composition of gastric cancer, fill under aseptic condition (specification is 100ml/ bottle) must be treated.
Experimental example 1: the action effect of immunity of organisms is tested by the fermented tcm compositions of the present invention
The action effect of immunity of organisms is measured by this experiment mainly for thing of the present invention；Control sample is and the preferred unleavened high pressure extract thing of Chinese medicinal components scheme same concentrations；Experiment medicine used and instrument are commercially available.
The preparation of 1.1 mice spleen suspensions
By Balb/c sacrifice, with 75% alcohol-pickled 5 minutes, the complete spleen of aseptic separation, washes away floating blood with PBS, divest excess tissue composition, inhale 5mlPBS with syringe, insert in spleen, blowout cell, repeats transparent to spleen adventitia for several times, and residue spleen tissue is cut into size 1mm3Fritter, shred and be placed on 200 mesh filter screens, grind with syringe nook closing member, PBS washs, collect cleaning mixture, centrifugal 5 minutes of 1200rpm, abandon supernatant, add 3ml erythrocyte cracked liquid, stand 2 minutes, add 10mlPBS and terminate reaction, centrifugal 5 minutes of 1200rpm, abandon supernatant, PBS washes twice, centrifugal 5 minutes of 1200rpm, wash once by the incomplete culture medium of RPMI-1640, centrifugal 5 minutes of 1200rpm, abandon supernatant, add appropriate RPMI-1640 complete medium (containing 10% hyclone, 100u/ml penicillin, 100ug/ml streptomycin and 10mMHepes), adjust cell concentration to 2 × 104Individual cell/ml, Trypan Blue, detection cell survival rate is more than 95%.
1.2 fermented tcm compositionss of the present invention are on lymphopoietic impact
Fermented tcm compositions A, B, C and the matched group pure water embodiment 1 ~ 3 prepared carry out gradient dilution respectively, obtain respective gradient dilution liquid.Above-mentioned splenocyte suspension is joined in 96 well culture plates by 100ul/ hole, then adds 10ulConA(5ug/ml respectively), the gradient dilution liquid of the 10ul present composition, each gradient does 6 repetitions, and culture plate is placed in 37 DEG C, 5%CO2Incubator in cultivate 72h；Blank (adding 10ul pure water) is set simultaneously.Adopting CCK-8 kit method to measure the impact (representing with stimulation index SI) on spleen lymphocyte proliferation ability of each fermented product, result is as shown in table 1.
Table 1 treats the probiotics fermention Chinese medicine composition of gastric cancer to lymphopoietic impact
Stimulation index SI value and splenocyte how much being proportionate property, as shown in Table 1, compared with the unfermentable Chinese medicine extraction liquid of matched group, the SI value of the fermented tcm compositions of the present invention is all higher than 4, apparently higher than matched group, illustrate that it can dramatically increase the quantity of mouse T lymphocyte, improve immunity of organisms.
Experimental example 2: Growth of Gastric inhibition is tested by the fermented tcm compositions of the present invention
Growth of Gastric inhibition is measured by this experiment mainly for the fermented tcm compositions of the present invention；Control sample is and the preferred unfermentable high pressure extract thing of Chinese medicinal components scheme same concentrations；Drug level is calculated by containing dry medical material gauge, and animal dosage raises animal with quite 10 times of drug port with clinical equivalent dosage, and dosage is big or hour carries out suitably concentration or dilutes；Experiment medicine used and instrument are commercially available.
The preparation of 2.1 pastille animal serums
Rat 196, male, body weight 250 ~ 300g/ only, is randomly divided into 14 groups, often group 14, gastric infusion；(1) blank group, fills the normal saline with experimental group same dose；(2) experimental group be fermentation group (fermented tcm compositions A, fermented tcm compositions B, fermented tcm compositions C), group of not fermenting (drug control group) (basic, normal, high concentration respectively 3.235,6.470,12.94g/kg 7d), dosage 2ml/ times/day, continuous 7 days, administration takes blood after 6 hours every time, and each two repeat；(3) 5-fluorouracil (5-Fu) positive controls, 50mg/kg 7d, lumbar injection；All broken end takes blood, in 4 DEG C, the centrifugal 20min of 2000r/min, separates serum, and subpackage after 56 DEG C of inactivation 30min ,-20 DEG C frozen standby.
2.2 cell inhibitory effect test (MTT reducing process)
Taking the logarithm the SGC-7901 cell of trophophase, adjusting concentration of cell suspension with the RPMI-1640 culture fluid containing 10% hyclone is 2.0 × 104Individual/L inoculating cell, in 96 well culture plates, every hole 200ul, puts 37 DEG C, 5%CO2, after cultivating 24h in the incubator of saturated humidity, change the RPMI-1640 without hyclone and be further cultured for 24h, make cell synchronization；Abandoning supernatant, each group is changed with the RPMI-1640 culture fluid containing 10% corresponding rat blood serum respectively, and often group sets 6 multiple holes and cultivates 48h；Every hole adds 5mg/mlMTT solution 20ul again, continues to hatch 4h, adds 200ulDMSO after abandoning supernatant, micro oscillator vibration 10min, measures 490nm wavelength absorbance value (OD) by microplate reader immediately, calculates cell inhibitory rate.
Cell inhibitory rate (IR)=1-(experimental group mean absorbance values/blank group mean absorbance values) } × 100%.
2.3 fermented tcm compositionss of the present invention affect result to what SGC-7901gastriccarcinomacellline strain was bred:
As shown in Table 2, the suppression ratio of stomach cancer cell is 66% by positive controls (5-Fu), stomach cancer cell suppression ratio is about 65% by fermentation group (height), suppression ratio is more or less the same compared with existing chemicals (5-Fu), stomach cancer cell suppression ratio is about 51% by group of not fermenting (height), fermentation group, higher than not fermenting group, illustrates this compositions therapeutic effect better (the fermentation composition C effect of embodiment 3 preparation is best) to gastric cancer；The suppression of stomach cancer cell is reached the highest when being administered the serum of 5 days and maintains balance afterwards by positive controls (5-Fu), stomach cancer cell is suppressed reach the highest when being administered the serum of 4 days and maintain balance afterwards by fermentation group, stomach cancer cell is suppressed always stable by group of not fermenting, illustrate fermentation composition of the present invention absorb compared with chemicals, group of not fermenting rapidly, instant effect.
The suppression ratio (%) that SGC-7901gastriccarcinomacellline strain is bred by table 2 present composition
Note: time in table (my god) it is administered natural law for rat.
Claims (10)
1. the probiotics fermention Chinese medicine composition treating gastric cancer, it is characterised in that described Chinese medicine composition is made up of the medical material of following weight portion: Radix Panacis Quinquefolii 3 ~ 6 parts, Radix Bupleuri 3 ~ 10 parts, Fructus Aurantii 3 ~ 10 parts, Radix Scutellariae 3 ~ 10 parts, the Rhizoma Pinelliae 3 ~ 9 parts, Herba Salviae Chinensis 5 ~ 20 parts, 5 ~ 10 parts of the Piao sheath in sea, Bulbus Fritillariae Thunbergii 2 ~ 8 parts, the Radix Paeoniae Alba 6 ~ 15 parts, Rhizoma Chuanxiong 3 ~ 10 parts, Rhizoma Cyperi (processed with vinegar) 6 ~ 10 parts, Rhizoma Alpiniae Officinarum 3 ~ 6 parts, Radix Angelicae Sinensis 6 ~ 12 parts, Herba Cistanches 6 ~ 10 parts, Radix Glycyrrhizae Preparata 2 ~ 10 parts；Its preparation method is as follows:
(1) medical material processes:
A. Radix Scutellariae, Herba Salviae Chinensis add 8 ~ water of 15 times of medical material weight together, soak 20 ~ 60 minutes, carry 30 ~ 60 minutes through 115 ~ 121 DEG C of water under high pressures, filter, filtrate sterilizing, for aseptic Radix Scutellariae, Herba Salviae Chinensis mixed liquor, standby；
B. Radix Angelicae Sinensis adds 8 ~ water of 15 times of medical material weight, distills after soaking 20 ~ 60 minutes, collects the aqueous solution after distillate and distillation respectively, and distillate seals, 115 ~ 121 DEG C of sterilizings 30 ~ 60 minutes, obtains aseptic distillate, standby；
C. by adding 8 together with 12 kinds of medical materials of Radix Angelicae Sinensis medicinal residues and all the other ~ water of 15 times of medical material weight, soak 20 ~ 60 minutes, carry 30 ~ 60 minutes through 115 ~ 121 DEG C of water under high pressures, filter, filtrate merges with the aqueous solution after Radix Angelicae Sinensis distillation, obtains herbal extract；
(2) enzymolysis: by step (1) the C herbal extract obtained, maintain 70 ~ 95 DEG C of constant temperature, regulate pH to 6.0 ~ 7.0, increase temperature amylase, enzyme digestion reaction 10 ~ 15 minutes, with iodine liquid for indicator, medicinal liquid meet iodine liquid constant blue time, enzymolysis terminates, and heats to 100 DEG C of enzyme denaturing 10 ~ 20 minutes, filtering, filtrate is standby；
(3) concentration, dispensing: after the filtrate reduced in volume obtain step (2), add fructose, peptone, yeast extract, yeast powder, adds inorganic salt, adds sweeting agent, regulates pH value to 6.5 ~ 7.5, and 115 ~ 121 DEG C of sterilizings 30 ~ 60 minutes obtain sterile liquid medicine；
(4) inoculation, fermentation: when the sterile liquid medicine temperature of step (3) is down to 25 ~ 35 DEG C, the aseptic distillate of (1) B identical temperature obtained is mixed with, inoculate the Leuconostoc mesenteroides bacterium solution that pre-incubated percent by volume is 1% ~ 5%, 25 ~ 35 DEG C of cultivations, when pH value is down to 5.5 ~ 6.5, add glucose, high temperature sterilize, treat that temperature is down to 35 ~ 40 DEG C, inoculate a kind of bacillus bifidus bacterium solution that pre-incubated percent by volume is 1% ~ 5%, cultivate 6 ~ 8 hours for 37 DEG C, again inoculate a kind of lactobacillus bacterium solution that pre-incubated percent by volume is 1% ~ 5%, continue fermentation 20 ~ 24 hours；When pH value is down to 4.0 ~ 4.1, reduce fermentation temperature to 20 ~ 30 DEG C, continue fermentation to pH value and be down to 3.5 ~ 3.8, terminate fermentation, obtain Chinese medicine composition fermentation liquid；
(5) allotment, inactivation: in the Chinese medicine composition fermentation liquid that (4) obtain, add aseptic Radix Scutellariae, Herba Salviae Chinensis mixed liquor that aseptic dietary fiber, prebiotics and step (1) A extract, mixing, 115 ~ 121 DEG C of sterilizings 5 ~ 50 minutes, the probiotics fermention Chinese medicine composition of the gastric cancer that namely obtains medical treatment.
2. the probiotics fermention Chinese medicine composition for the treatment of gastric cancer according to claim 1, it is characterised in that preferred Chinese medicine composition be made up of the medical material of following weight portion: Radix Panacis Quinquefolii 3 parts, Radix Bupleuri 6 parts, Fructus Aurantii 6 parts, Radix Scutellariae 6 parts, the Rhizoma Pinelliae 5 parts, Herba Salviae Chinensis 12 parts, 10 parts of the Piao sheath in sea, Bulbus Fritillariae Thunbergii 4 parts, the Radix Paeoniae Alba 9 parts, Rhizoma Chuanxiong 5 parts, Rhizoma Cyperi (processed with vinegar) 6 parts, Rhizoma Alpiniae Officinarum 4 parts, Radix Angelicae Sinensis 9 parts, Herba Cistanches 10 parts, Radix Glycyrrhizae Preparata 2 parts.
3. the probiotics fermention Chinese medicine composition for the treatment of gastric cancer according to claim 1, it is characterised in that described amylase is α-alpha-amylase, its Dosage calculation method is: medical material amount × 30/ enzyme unit alive, is first mixed by enzyme with a small amount of water with front.
4. the probiotics fermention Chinese medicine composition for the treatment of gastric cancer according to claim 1, it is characterised in that the consumption of described fructose is weight g/ volume ml percentage ratio 0.1 ~ 1.0%, the consumption of described glucose is weight g/ volume ml percentage ratio is 0.1 ~ 1.0%.
5. the probiotics fermention Chinese medicine composition for the treatment of gastric cancer according to claim 1, it is characterized in that, the consumption of described peptone be weight g/ volume ml percentage ratio 0.05 ~ 1.5%, yeast extract consumption be weight g/ volume ml percentage ratio 0.01 ~ 0.5%, yeast powder consumption be weight g/ volume ml percentage ratio 0.01 ~ 0.5%；Described sweeting agent is the sucralose of the sucrose of weight g/ volume ml percentage ratio 3% and weight g/ volume ml percentage ratio 0.007%.
6. the probiotics fermention Chinese medicine composition for the treatment of gastric cancer according to claim 1, it is characterised in that described inorganic salt is K2HPO4·3H2O0.1g/100ml、KH2PO40.1g/100ml、FeSO4·7H2O5.0mg/100ml、ZnSO4·7H2O4.4mg/100ml、MgSO4·7H2O25mg/100ml。
7. the probiotics fermention Chinese medicine composition for the treatment of gastric cancer according to claim 1, it is characterised in that described dietary fiber is weight g/ volume ml percentage ratio is the polydextrose of 1 ~ 5%；Described prebiotics is weight g/ volume ml percentage ratio be 0.1 ~ 0.7% oligofructose slurry G55L.
8. the probiotics fermention Chinese medicine composition for the treatment of gastric cancer according to claim 1, it is characterised in that described Leuconostoc mesenteroides is selected from Leuconostoc mesenteroides dextran subspecies or leuconostoc mesenteroides sub species cremoris；Described bacillus bifidus is selected from bifidobacterium bifidum, bifidobacterium breve, bifidobacterium adolescentis or bifidobacteria infantis；Described lactobacillus is selected from bacillus acidophilus, Deshi Lactobacillus, lactobacillus casei, lactobacillus rhamnosus, Lactobacillus reuteri or Lactobacillus plantarum.
9. the preparation method of the probiotics fermention Chinese medicine composition treating gastric cancer, it is characterised in that step is as follows:
(1) medical material processes:
A. Radix Scutellariae, Herba Salviae Chinensis add 8 ~ water of 15 times of medical material weight together, soak 20 ~ 60 minutes, carry 30 ~ 60 minutes through 115 ~ 121 DEG C of water under high pressures, filter, filtrate sterilizing, for aseptic Radix Scutellariae, Herba Salviae Chinensis mixed liquor, standby；
B. Radix Angelicae Sinensis adds 8 ~ water of 15 times of medical material weight, distills after soaking 20 ~ 60 minutes, collects the aqueous solution after distillate and distillation respectively, and distillate seals, 115 ~ 121 DEG C of sterilizings 30 ~ 60 minutes, obtains aseptic distillate, standby；
C. by adding 8 together with 12 kinds of medical materials of Radix Angelicae Sinensis medicinal residues and all the other ~ water of 15 times of medical material weight, soak 20 ~ 60 minutes, carry 30 ~ 60 minutes through 115 ~ 121 DEG C of water under high pressures, filter, filtrate merges with the aqueous solution after Radix Angelicae Sinensis distillation, obtains herbal extract；
(2) enzymolysis: by step (1) the C herbal extract obtained, maintain 70 ~ 95 DEG C of constant temperature, regulate pH to 6.0 ~ 7.0, increase temperature amylase, enzyme digestion reaction 10 ~ 15 minutes, with iodine liquid for indicator, medicinal liquid meet iodine liquid constant blue time, enzymolysis terminates, and heats to 100 DEG C of enzyme denaturing 10 ~ 20 minutes, filtering, filtrate is standby；
(3) concentration, dispensing: after the filtrate reduced in volume obtain step (2), add fructose, peptone, yeast extract, yeast powder, adds inorganic salt, adds sweeting agent, regulates pH value to 6.5 ~ 7.5, and 115 ~ 121 DEG C of sterilizings 30 ~ 60 minutes obtain sterile liquid medicine；
(4) inoculation, fermentation: when the sterile liquid medicine temperature of step (3) is down to 25 ~ 35 DEG C, the aseptic distillate of (1) B identical temperature obtained is mixed with, inoculate the Leuconostoc mesenteroides bacterium solution that pre-incubated percent by volume is 1% ~ 5%, 25 ~ 35 DEG C of cultivations, when pH value is down to 5.5 ~ 6.5, add glucose, high temperature sterilize, treat that temperature is down to 35 ~ 40 DEG C, inoculate a kind of bacillus bifidus bacterium solution that pre-incubated percent by volume is 1% ~ 5%, cultivate 6 ~ 8 hours for 37 DEG C, again inoculate a kind of lactobacillus bacterium solution that pre-incubated percent by volume is 1% ~ 5%, continue fermentation 20 ~ 24 hours；When pH value is down to 4.0 ~ 4.1, reduce fermentation temperature to 20 ~ 30 DEG C, continue fermentation to pH value and be down to 3.5 ~ 3.8, terminate fermentation, obtain Chinese medicine composition fermentation liquid；
(5) allotment, inactivation: in the Chinese medicine composition fermentation liquid that (4) obtain, add aseptic Radix Scutellariae, Herba Salviae Chinensis mixed liquor that aseptic dietary fiber, prebiotics and step (1) A extract, mixing, 115 ~ 121 DEG C of sterilizings 5 ~ 50 minutes, the probiotics fermention Chinese medicine composition of the gastric cancer that namely obtains medical treatment.
10. the probiotics fermention Chinese medicine composition of the treatment gastric cancer described in claim 1-8 suppress Growth of Gastric in preparation, improve immunity, regulating intestinal canal colony balance, the application that improves in the medicine of gastrointestinal function.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2021109879A1 (en) | 2019-12-06 | 2021-06-10 | 倪健伟 | Composition having wholesome personalized intestinal flora diversity function and application |
| CN113481243A (en) * | 2021-08-16 | 2021-10-08 | 兰州大学 | Angelica sinensis fermentation liquor with effects of resisting oxidation, resisting aging and regulating intestinal flora, and preparation method and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101926949A (en) * | 2009-06-25 | 2010-12-29 | 天津太平洋制药有限公司 | Preparation process of galangal and rhizoma cyperi liquid capsule |
| CN102670729A (en) * | 2012-05-16 | 2012-09-19 | 乌鲁木齐市生命核力高科有限公司 | Preparation method for cistanche tubulosa and American ginseng compound capsule |
| CN104069194A (en) * | 2013-03-26 | 2014-10-01 | 成都地奥集团天府药业股份有限公司 | A traditional Chinese medicine composition having anticancer effects and a preparing method and uses thereof |
| CN105286010A (en) * | 2015-09-17 | 2016-02-03 | 周学义 | Ferment capable of maintaining beauty and keeping young, and production method thereof, as well as oral liquid capable of maintaining beauty and keeping young, and production method thereof |
-
2016
- 2016-03-30 CN CN201610189936.2A patent/CN105749099B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101926949A (en) * | 2009-06-25 | 2010-12-29 | 天津太平洋制药有限公司 | Preparation process of galangal and rhizoma cyperi liquid capsule |
| CN102670729A (en) * | 2012-05-16 | 2012-09-19 | 乌鲁木齐市生命核力高科有限公司 | Preparation method for cistanche tubulosa and American ginseng compound capsule |
| CN104069194A (en) * | 2013-03-26 | 2014-10-01 | 成都地奥集团天府药业股份有限公司 | A traditional Chinese medicine composition having anticancer effects and a preparing method and uses thereof |
| CN105286010A (en) * | 2015-09-17 | 2016-02-03 | 周学义 | Ferment capable of maintaining beauty and keeping young, and production method thereof, as well as oral liquid capable of maintaining beauty and keeping young, and production method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 袁可淼: "尤建良教授从"四不和"辨治胃癌的临证思路与经验简介", 《新中医》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021109879A1 (en) | 2019-12-06 | 2021-06-10 | 倪健伟 | Composition having wholesome personalized intestinal flora diversity function and application |
| CN113481243A (en) * | 2021-08-16 | 2021-10-08 | 兰州大学 | Angelica sinensis fermentation liquor with effects of resisting oxidation, resisting aging and regulating intestinal flora, and preparation method and application thereof |
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| CN105749099B (en) | 2019-09-13 |
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