CN105797003A - Probiotics fermentation traditional Chinese medicine compound composition for treating colorectal cancer and preparation method and application thereof - Google Patents
Probiotics fermentation traditional Chinese medicine compound composition for treating colorectal cancer and preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a probiotics fermentation traditional Chinese medicine compound composition for treating colorectal cancer. The composition is prepared through the method that pectinase is added for enzymolysis after scutellaria baicalensis, dried rehamnnia roots, radix sophorae flavescentis, radix scrophulariae, all grass of patrina villosa juss, spreading hedyotis herb, moutan barks, fructus aurantii, herba cistanche, bittersweet herb, American ginseng, scorch-fired hawthorn fruits, sargentgloryvine stem and coix seeds are subjected to water extraction, high-temperature enzyme deactivation is conducted, nutrients, inorganic salt and the like with different ratios are added, high-temperature sterilization is conducted, and the mixture is inoculated with composite intestinal probiotics for fermentation. Mice experiments show that the composition can effectively restrain increasing of solid tumors of the colorectal cancer, and the activity for restraining the solid tumors SW1116 of the adenocarcinoma of colon of the composition is obviously improved. The composition can be taken by a patient conveniently, can prolong the lifetime of the patient, improve the immunity of the patient and improve the life quality of the patient, has remarkably curative effect on intestinal cancer treatment, and is safe, effective and free of toxic and side effects.
Description
Technical field
The present invention relates to the fermentation composition that a kind of Chinese medicine extract prepares through intestinal probiotics fermention and the application that this fermentation composition is in preparing the medicine for treating colon cancer, belong to technical field of biological fermentation.
Background technology
Colon cancer is the common malignant tumor of digestive tract betiding colon site, is apt to occur in rectum and sigmoid colon intersection, and the highest with 40～50 years old age group sickness rate, the ratio of men and women is 2～3:1.In China, the M & M of colon cancer has risen to the 4th now and the 5th, there are about 200,000 people every year and dies from colon cancer.This means that tumor cell still can not thoroughly be eradicated by current treatment.
For early stage colorectal cancer patients, can colon cancer be cured and be mainly dependent on whether to take appropriate treatment means in time.In general, early stage colon cancer cancerous protuberance is less, and diffusion transfer does not occur, and its maximally efficient treatment means is excision, and 5 years survival rates after early stage colon cancer excision are up to more than 60%.In general, adopt traditional Chinese medical science cancer therapy drug after treatment after colon cancer Operation in early stage, can effectively reduce the generation of post-operative complication, reduce postoperative metastasis and recurrence.For being difficult to the colon cancer early treatment of tolerance excision, chemicotherapy can being taked directly to kill the Therapeutic mode of cancerous cell, chemicotherapy is applied relatively broad in the early treatment of colon cancer, and Therapeutic Results of Combination Chemotherapy is more preferably.But how very poor the or elderly patient for body constitution, cannot bear the side effect of chemicotherapy, therefore in the early treatment of colon cancer, chemicotherapy with treatment by Chinese herbs combined treatment, to strengthen patient's autoimmunity, and need to alleviate the side effect of chemicotherapy.Chinese medicine decoction is the organic conception using theory of Chinese medical science, determination for the treatment of based on pathogenesis obtained through differentiation of symptoms and signs, determined curative effect, but traditional Chinese medicine decoction taste is extremely bitter, especially the colorectal cancer patients after radiotherapy, health is not good, is difficult to especially swallow, there is certain limitation, and effective slowly, poor effect.Therefore, to be safely and effectively suitable to the Chinese medicine preparation treatment colon cancer that patient takes significant in exploitation.
Summary of the invention
For overcoming the deficiencies in the prior art, it is an object of the present invention to provide a kind of probiotics fermention Traditional Chinese medicine compound composition treating colon cancer, said composition is prepared after composite probiotics ferment by traditional Chinese medicine compound extract.The technical scheme adopted is: Chinese medicine compound medical material adds pectinase enzymatic hydrolysis after water carries, high temperature enzyme denaturing, add nutrient and the inorganic salt etc. of different ratio, high temperature sterilize, access compound intestinal probiotics fermention rear stabilization and process and obtain the probiotics fermention Traditional Chinese medicine compound composition of the present invention.
Described Chinese medicine compound is made up of the Chinese medicine ingredients of following weight portion: Radix Scutellariae 12～40 parts, Radix Rehmanniae 40～60 parts, Radix Sophorae Flavescentis 18～36 parts, Radix Scrophulariae 24～60 parts, Herba Patriniae 36～60 parts, Herba Hedyotidis Diffusae 40～120 parts, Cortex Moutan 12～48 parts, Fructus Aurantii 12～40 parts, Herba Cistanches 24～40 parts, Herba Solani Lyrati 36～120 parts, Radix Panacis Quinquefolii 8.0～24 parts, Fructus Crataegi (parched to brown) 12～48 parts, Caulis Sargentodoxae 40～120 parts, Semen Coicis 36～120 parts.
Preferably, described Chinese medicine compound is made up of the Chinese medicine ingredients of following weight portion: Radix Scutellariae 36 parts, Radix Rehmanniae 60 parts, Radix Sophorae Flavescentis 36 parts, Radix Scrophulariae 36 parts, Herba Patriniae 60 parts, Herba Hedyotidis Diffusae 48 parts, Cortex Moutan 24 parts, Fructus Aurantii 24 parts, Herba Cistanches 40 parts, Herba Solani Lyrati 60 parts, Radix Panacis Quinquefolii 8.0 parts, Fructus Crataegi (parched to brown) 20 parts, Caulis Sargentodoxae 48 parts, Semen Coicis 48 parts.
The preparation method that it is a further object to provide the probiotics fermention Traditional Chinese medicine compound composition of the treatment colon cancer of the present invention, its step is as follows:
A. above 14 taste Chinese medicines, take Radix Panacis Quinquefolii powder and are broken into coarse powder, and fineness is 20 order～40 orders, independent boiling twice, each 1 hour, and filtrate merges, standby；Cortex Moutan add water distillation prepare distillate standby；The medicinal residues of Radix Panacis Quinquefolii and Cortex Moutan and other Chinese medicine boiling twice, first time adds 12 times amount water soaking 1.0 hours, decoct, keep micro-after boiling to boil 2.0 hours, second time adds 10 times amount soak by water, keep micro-after boiling to boil 1.0 hours, merging filtrate, filtrate is regulated pH to 6.0, temperature is to 50 DEG C, add a certain amount of pectase, stir, enzymolysis 2 hours, terminate enzymolysis, 100 DEG C of enzyme denaturing 10min, enzymolysis solution and Radix Panacis Quinquefolii decoction liquor are merged that to be concentrated at 60 DEG C relative density be 1.03～1.07, then concentrated solution and Cortex Moutan distillate are merged to obtain traditional Chinese medicine compound extract；
B. in above-mentioned traditional Chinese medicine compound extract, the glucose of different ratio, peptone, yeast extract, yeast powder and inorganic salt are added, add or without sweeting agent, constant volume, regulate pH value to 7.0, in 115 DEG C of autoclave sterilization 40min, obtain aseptic Chinese medicinal compound extract；
C. when above-mentioned aseptic Chinese medicinal compound extract temperature is down to 39 DEG C, inoculate one or both and above bacillus bifidus bacterium solution that pre-incubated each percent by volume is 0.5%～1.0%, after cellar culture about 2～8 hours, again inoculate one or both and above lactobacillus bacterium solution that pre-incubated each percent by volume is 0.5%～1.0%, continue cultivation 23～28 hours；Or inoculate one or both and above bacillus bifidus bacterium solution that pre-incubated each percent by volume is 0.5%～1.0% simultaneously and each percent by volume is 0.5%～1.0% one or both and above lactobacillus bacterium solution, ferment 26～40 hours, when pH value is down to below 4.1, terminate fermentation, stabilizing treatment, obtains described probiotics fermention Traditional Chinese medicine compound composition.
Described bacillus bifidus is selected from bifidobacterium bifidum, bifidobacterium breve, bifidobacterium adolescentis or bifidobacteria infantis；Described lactobacillus is selected from bacillus acidophilus, Deshi Lactobacillus, Lactobacillus plantarum, lactobacillus casei or lactobacillus rhamnosus.Strain derives from China typical culture collection center (CCTCC) (China, Wuhan) or China General Microbiological culture presevation administrative center (CGMCC) (China, Beijing) or separate from the feces or intestinal submucosa tissue of normal person and obtain these bacterial isolateses.
Pectase addition described in step A is 0.1ml/100ml, the addition of the glucose described in step B is 0.7g/100ml, the addition of described yeast extract is 0.13g/100ml, and the addition of described yeast powder is 0.07g/100ml, and the addition of described peptone is 0.4g/100ml.
Described inorganic salt is K2HPO4·3H2O0.1g/100ml、KH2PO40.1g/100ml, ferrous sulfate 7H2O5.0mg/100ml, zinc sulfate 7H2O4.4mg/100ml, magnesium sulfate 7H2O50mg/100ml。
For improving the mouthfeel of compositions, can add sweeting agent, its kind and addition in preparation method step B is sucrose 3.0g/100ml and/or sucralose 0.008g/100ml.
It is also another object of the present invention to provide the probiotics fermention Traditional Chinese medicine compound composition of the present invention application in the medicine of preparation treatment colon cancer.
The probiotics fermention Traditional Chinese medicine compound composition of the present invention can also add one or more and have identical, similar or different biological is active, and to the existing auxiliary of primary activity component list or synergism, but the mutually not naturally occurring or synthetic other drug composition of antagonism or its mixture again to each other, is applied to the patient needing this treatment in the way of oral and parenteral administration.During for being administered orally, liquid preparation such as water or oil-suspending agent or other liquid preparation such as mixture, Emulsion, syrup etc. can be made into.
Also can by the probiotics fermention Traditional Chinese medicine compound composition of the present invention by suitable drying means, prepare pressed powder, add one or more and have identical, similar or different biological is active, and to the existing auxiliary of primary activity component list or synergism, but the mutually not naturally occurring or synthetic other drug composition of antagonism or its mixture again to each other, is configured to be suitable to the medicine of the dosage forms such as the capsule of oral administration, tablet, granule.
Beneficial effect:
1, the probiotics fermention Traditional Chinese medicine compound composition of the treatment colon cancer of the present invention facilitates patients, and can extend the vital stage of patient, improves the immunity of patient, improves the quality of life of patient, and safely and effectively, have no side effect.
2, Chinese medicine compound involved in the present invention can effectively suppress the growth of the solid tumor of colon cancer, and preparation method involved in the present invention can strengthen the curative effect of involved compound of Chinese medicine colon cancer.
Detailed description of the invention
Embodiment 1: the preparation of probiotics fermention Traditional Chinese medicine compound composition A
Medical material, Radix Scutellariae 36g, Radix Rehmanniae 60g, Radix Sophorae Flavescentis 36g, Radix Scrophulariae 36g, Herba Patriniae 60g, Herba Hedyotidis Diffusae 48g, Cortex Moutan 24g, Fructus Aurantii 24g, Herba Cistanches 40g, Herba Solani Lyrati 60g, Radix Panacis Quinquefolii 8.0g, Fructus Crataegi (parched to brown) 20g, Caulis Sargentodoxae 48g, Semen Coicis 48g is weighed by following recipe quantity.Above 14 taste Chinese medicines, take Radix Panacis Quinquefolii powder and are broken into coarse powder, and fineness is 30 orders, independent boiling twice, each 1 hour, and filtrate merges, standby；Cortex Moutan add water distillation prepare distillate standby；The medicinal residues of Radix Panacis Quinquefolii and Cortex Moutan and other Chinese medicine boiling twice, first time adds 12 times amount water soaking 1.0 hours, decoct, keep micro-after boiling to boil 2.0 hours, second time adds 10 times amount soak by water, keep micro-after boiling to boil 1.0 hours, merging filtrate, filtrate is regulated pH to 6.0, temperature is to 50 DEG C, add 10ml pectase, stir, enzymolysis 2 hours, terminate enzymolysis, 100 DEG C of enzyme denaturing 10min, merge enzymolysis solution and Radix Panacis Quinquefolii decoction liquor that to be concentrated at 60 DEG C relative density be 1.053, and concentrated solution and Cortex Moutan distillate are merged to obtain traditional Chinese medicine compound extract；Glucose 7.0g, yeast extract 1.3g, yeast powder 0.7g, peptone 4.0g, inorganic salt (K is added in above-mentioned traditional Chinese medicine compound extract2HPO4·3H2O1.0g、KH2PO41.0g, ferrous sulfate 7H2O50mg, zinc sulfate 7H2O44mg, magnesium sulfate 7H2O500mg), sucrose 35g, sucralose 0.08g, it is settled to 1000ml, regulates pH value to 7.0, in 115 DEG C of autoclave sterilization 40min, obtain aseptic Chinese medicinal compound extract；When above-mentioned aseptic Chinese medicinal compound extract temperature is down to 39 DEG C, inoculating pre-incubated percent by volume is respectively bifidobacterium bifidum (normal human's separation of 0.5%, identify through Institute of Microorganism, Academia Sinica) and bifidobacterium breve (AS1.2213) bacterium solution, after cellar culture about 5 hours, again inoculate Lactobacillus plantarum (AS1.19) bacterium solution that pre-incubated percent by volume is 0.7%, continue to cultivate 26 hours, when pH value is down to below 4.1, terminate fermentation, stabilizing treatment, obtains described probiotics fermention Traditional Chinese medicine compound composition A.
Embodiment 2: the preparation of probiotics fermention Traditional Chinese medicine compound composition B
Medical material, Radix Scutellariae 12g, Radix Rehmanniae 60g, Radix Sophorae Flavescentis 18g, Radix Scrophulariae 60g, Herba Patriniae 36g, Herba Hedyotidis Diffusae 120g, Cortex Moutan 12g, Fructus Aurantii 40g, Herba Cistanches 24g, Herba Solani Lyrati 120g, Radix Panacis Quinquefolii 8.0g, Fructus Crataegi (parched to brown) 48g, Caulis Sargentodoxae 40g, Semen Coicis 120g is weighed by following recipe quantity.Above 14 taste Chinese medicines, take Radix Panacis Quinquefolii powder and are broken into coarse powder, and fineness is 20 orders, independent boiling twice, each 1 hour, and filtrate merges, standby；Cortex Moutan add water distillation prepare distillate standby；The medicinal residues of Radix Panacis Quinquefolii and Cortex Moutan and other Chinese medicine boiling twice, first time adds 12 times amount water soaking 1.0 hours, decoct, keep micro-after boiling to boil 2.0 hours, second time adds 10 times amount soak by water, keep micro-after boiling to boil 1.0 hours, merging filtrate, filtrate is regulated pH to 6.0, temperature is to 50 DEG C, add 15ml pectase, stir, enzymolysis 2 hours, terminate enzymolysis, 100 DEG C of enzyme denaturing 10min, merge enzymolysis solution and Radix Panacis Quinquefolii decoction liquor that to be concentrated at 60 DEG C relative density be 1.056, and concentrated solution and Cortex Moutan distillate are merged to obtain traditional Chinese medicine compound extract；Glucose 7.0g, yeast extract 1.3g, yeast powder 0.7g, peptone 4.0g, inorganic salt (K is added in above-mentioned traditional Chinese medicine compound extract2HPO4·3H2O1.0g、KH2PO41.0g, ferrous sulfate 7H2O50mg, zinc sulfate 7H2O44mg, magnesium sulfate 7H2O500mg), it is settled to 1000ml, regulates pH value to 7.0, in 115 DEG C of autoclave sterilization 40min, obtain aseptic Chinese medicinal compound extract；When above-mentioned aseptic Chinese medicinal compound extract temperature is down to 39 DEG C, inoculate bifidobacterium adolescentis (AS1.2190) bacterium solution that pre-incubated percent by volume is 1.0%, after cellar culture about 3 hours, again inoculating pre-incubated percent by volume is respectively bacillus acidophilus (AS1.1854) and Deshi Lactobacillus (AS1.1480) bacterium solution of 0.5%, continue to cultivate 27 hours, when pH value is down to below 4.1, terminate fermentation, stabilizing treatment, obtains described probiotics fermention Traditional Chinese medicine compound composition B.
Embodiment 3: the preparation of probiotics fermention Traditional Chinese medicine compound composition C
Medical material, Radix Scutellariae 40g, Radix Rehmanniae 40g, Radix Sophorae Flavescentis 36g, Radix Scrophulariae 24g, Herba Patriniae 60g, Herba Hedyotidis Diffusae 40g, Cortex Moutan 48g, Fructus Aurantii 12g, Herba Cistanches 40g, Herba Solani Lyrati 36g, Radix Panacis Quinquefolii 24g, Fructus Crataegi (parched to brown) 12g, Caulis Sargentodoxae 120g, Semen Coicis 36g is weighed by following recipe quantity.Above 14 taste Chinese medicines, take Radix Panacis Quinquefolii powder and are broken into coarse powder, and fineness is 40 orders, independent boiling twice, each 1 hour, and filtrate merges, standby；Cortex Moutan add water distillation prepare distillate standby；The medicinal residues of Radix Panacis Quinquefolii and Cortex Moutan and other Chinese medicine boiling twice, first time adds 12 times amount water soaking 1.0 hours, decoct, keep micro-after boiling to boil 2.0 hours, second time adds 10 times amount soak by water, keep micro-after boiling to boil 1.0 hours, merging filtrate, filtrate is regulated pH to 6.0, temperature is to 50 DEG C, add 12ml pectase, stir, enzymolysis 2 hours, terminate enzymolysis, 100 DEG C of enzyme denaturing 10min, being concentrated at 60 DEG C by enzymolysis solution and Radix Panacis Quinquefolii decoction liquor relative density is 1.058, and concentrated solution and Cortex Moutan distillate are merged to obtain traditional Chinese medicine compound extract；Glucose 7.0g, yeast extract 1.3g, yeast powder 0.7g, peptone 4.0g, inorganic salt (K is added in above-mentioned traditional Chinese medicine compound extract2HPO4·3H2O1.0g、KH2PO41.0g, ferrous sulfate 7H2O50mg, zinc sulfate 7H2O44mg, magnesium sulfate 7H2O500mg), sucralose 0.08g, it is settled to 1000ml, regulates pH value to 7.0, in 115 DEG C of autoclave sterilization 40min, obtain aseptic Chinese medicinal compound extract；When above-mentioned aseptic Chinese medicinal compound extract temperature is down to 39 DEG C, inoculate pre-incubated percent by volume is respectively bifidobacteria infantis (AS1.853), lactobacillus rhamnosus (AS1.22) and Deshi Lactobacillus (AS1.1480) bacterium solution of 0.5% simultaneously, cellar culture 30 hours, when pH value is down to below 4.1, terminate fermentation, stabilizing treatment, obtains described probiotics fermention Traditional Chinese medicine compound composition C.
Experimental example: probiotics fermention Traditional Chinese medicine compound composition inhibitor against colon carcinoma cells of the present invention is tested
1, animal model
Set up human colon adenocarcinoma's little nude mice original position Implantation matastasis model.BALB/cnu/nu nude mouse, male, 4 ~ 6 weeks ages of Mus, about body weight 20g, raises when SPF level.Adopt human colon cancer cell strain SW1116, will containing 2 × 107The suspension 0.2ml of cancerous cell, in mouse bare subcutaneous injection, goes down to posterity into solid tumor after becoming tumor, and this group tumor source was the 5th generation.Put to death mice with tumor during plantation, take out Subcutaneous tumor, shred into 1.5mm diameter fragment standby.Experiment nude mice gives pentobarbital sodium (40mg/kg) intraperitoneal anesthesia, enters abdomen through median abdominal incision, clamps out caecum wall, cuts serous coat in cap end, tumor tissue sews up serous coat cutting section.
2, given the test agent and experiment packet
Given the test agent: probiotics fermention Traditional Chinese medicine compound composition A, B, C of preparation in embodiment.Given the test agent human body recommended amounts is the 240ml/(day for human beings), by 60kg human body weighing machine, i.e. 4.0ml/(kg day), the dose,equivalent of mice is equivalent to 10 times of human body recommended dose, by the 5 of this human body recommended dose times, 10 times, 20 times, A, B, C sets low dose group (A respectively1、B1、C1), middle dosage group (A2、B2、C2) and high dose group (A3、B3、C3), low dose group: 20ml/(kg day), middle dosage group: 40ml/(kg day), high dose group: 80ml/(kg day), the basic, normal, high dosage group (a of non-fermentation composition corresponding to preferred probiotics fermention Traditional Chinese medicine compound composition A is separately set1、a2、a3), matched group, often group 10.Starting after planting 1 week, matched group is from intraperitoneal injection of saline, and each administration group injects each test dose compositions of same volume, every day 1 time, shares 5 weeks.At the 6th weekend to plantation, 2 nude mices of matched group are dead because of lotus tumor exhaustion.
3, Testing index method
Tumor in situ tumor weight, tumour inhibiting rate: the 6th weekend after plantation, put to death animal by mice with tumor dislocation of cervical vertebra, measures tumor in situ tumor weight (W).Tumour inhibiting rate=(matched group W-experimental group W)/matched group W × 100%.
4, experimental project and result
The tumour inhibiting rate of each dosage group of probiotics fermention Traditional Chinese medicine compound composition of 4.1 present invention compares (see table 1)
As can be seen from the table, the mouse tumor weight of each dosage group of probiotics fermention Traditional Chinese medicine compound composition of the present invention compares with matched group, there is significant difference (P < 0.05), the tumour inhibiting rate that each low dose group is existing higher, middle high dose group tumour inhibiting rate is more notable, the probiotics fermention Traditional Chinese medicine compound composition of the visible present invention has good inhibiting effect for adenocarcinoma of colon SW1116 solid tumor, it is suppressed that rate reaches as high as more than 85%.
The tumour inhibiting rate of each dosage group of table 1 fermentation composition compare (± s)
* compare with matched group and have significant difference, P < 0.05.
Tumour inhibiting rate before and after 4.2 Chinese medicine compound fermentations involved in the present invention compares
The tumour inhibiting rate of each dosage group of the non-fermentation composition a of preferred probiotics fermention Traditional Chinese medicine compound composition A and correspondence thereof compares (result is in Table 2).The tumour inhibiting rate of high, medium and low each dosage group of fermentation group as can be seen from the table compares with corresponding dosage group of not fermenting, all having significant difference, Chinese medicine compound involved in the present invention suppresses the activity of adenocarcinoma of colon SW1116 solid tumor to be remarkably reinforced after probiotics fermention.
The tumour inhibiting rate of each dosage group of table 2A and a compare (± s)
▲ group of not fermenting with same dosage compares significant difference, P < 0.05.
5, conclusion
Experimental administration terminates rear matched group 2 dead mouses, each none dead mouse of dosage group of administration group, illustrates that Traditional Chinese medicine compound composition involved in the present invention can extend the vital stage of mice, improves the immunity of mice.Chinese medicine compound involved in the present invention can effectively suppress the growth of the solid tumor of colon cancer, and Chinese medicine compound involved in the present invention can significantly improve its activity suppressing adenocarcinoma of colon SW1116 solid tumor after probiotics fermention, illustrates that preparation method involved in the present invention enhances the curative effect of the treatment colon cancer of involved Traditional Chinese medicine compound composition.
Claims (10)
1. the probiotics fermention Traditional Chinese medicine compound composition treating colon cancer, it is characterized in that, be made up of the Chinese medicine compound of following weight portion: Radix Scutellariae 12～40 parts, Radix Rehmanniae 40～60 parts, Radix Sophorae Flavescentis 18～36 parts, Radix Scrophulariae 24～60 parts, Herba Patriniae 36～60 parts, Herba Hedyotidis Diffusae 40～120 parts, Cortex Moutan 12～48 parts, Fructus Aurantii 12～40 parts, Herba Cistanches 24～40 parts, Herba Solani Lyrati 36～120 parts, Radix Panacis Quinquefolii 8.0～24 parts, Fructus Crataegi (parched to brown) 12～48 parts, Caulis Sargentodoxae 40～120 parts, Semen Coicis 36～120 parts；Its preparation method is as follows:
A. taking Radix Panacis Quinquefolii powder and be broken into coarse powder, fineness is 20 order～40 orders, independent boiling twice, each 1 hour, and filtrate merges, standby；Cortex Moutan add water distillation prepare distillate standby；The medicinal residues of Radix Panacis Quinquefolii and Cortex Moutan and other Chinese medicine boiling twice, first time adds 12 times amount water soaking 1.0 hours, decoct, keep micro-after boiling to boil 2.0 hours, second time adds 10 times amount soak by water, keep micro-after boiling to boil 1.0 hours, merging filtrate, filtrate is regulated pH to 6.0, temperature is to 50 DEG C, add pectase, stir, enzymolysis 2 hours, terminate enzymolysis, 100 DEG C of enzyme denaturing 10min, merge enzymolysis solution and Radix Panacis Quinquefolii decoction liquor that to be concentrated at 60 DEG C relative density be 1.03～1.07, then concentrated solution and Cortex Moutan distillate are merged to obtain traditional Chinese medicine compound extract；
B. in above-mentioned traditional Chinese medicine compound extract, add glucose, peptone, yeast extract, yeast powder and inorganic salt, add or without sweeting agent, constant volume, regulate pH value to 7.0, in 115 DEG C of autoclave sterilization 40min, obtain aseptic Chinese medicinal compound extract；
C. when above-mentioned aseptic Chinese medicinal compound extract temperature is down to 39 DEG C, inoculating pre-incubated percent by volume is respectively one or both and the above bacillus bifidus bacterium solution of 0.5%～1.0%, after cellar culture about 2～8 hours, again inoculating pre-incubated percent by volume is respectively one or both and the above lactobacillus bacterium solution of 0.5%～1.0%, continues cultivation 23～28 hours；Or inoculate one or both and above bacillus bifidus bacterium solution that pre-incubated each percent by volume is 0.5%～1.0% simultaneously and each percent by volume is 0.5%～1.0% one or both and above lactobacillus bacterium solution, ferment 26～40 hours；When pH value is down to below 4.1, terminates fermentation, stabilizing treatment, obtain described probiotics fermention Traditional Chinese medicine compound composition.
2. the probiotics fermention Traditional Chinese medicine compound composition for the treatment of colon cancer according to claim 1, it is characterized in that, be made up of the Chinese medicine compound of following weight portion: Radix Scutellariae 36 parts, Radix Rehmanniae 60 parts, Radix Sophorae Flavescentis 36 parts, Radix Scrophulariae 36 parts, Herba Patriniae 60 parts, Herba Hedyotidis Diffusae 48 parts, Cortex Moutan 24 parts, Fructus Aurantii 24 parts, Herba Cistanches 40 parts, Herba Solani Lyrati 60 parts, Radix Panacis Quinquefolii 8.0 parts, Fructus Crataegi (parched to brown) 20 parts, Caulis Sargentodoxae 48 parts, Semen Coicis 48 parts.
3. the probiotics fermention Traditional Chinese medicine compound composition for the treatment of colon cancer according to claim 1, it is characterised in that the addition of described pectase is 0.1ml/100ml.
4. the probiotics fermention Traditional Chinese medicine compound composition for the treatment of colon cancer according to claim 1, it is characterised in that the addition of described glucose is 0.7g/100ml；The addition of described yeast extract is 0.13g/100ml；The addition of described yeast powder is 0.07g/100ml；The addition of described peptone is 0.4g/100ml.
5. the probiotics fermention Traditional Chinese medicine compound composition for the treatment of colon cancer according to claim 1, it is characterised in that described inorganic salt is: K2HPO4·3H2O0.1g/100ml、KH2PO40.1g/100ml, ferrous sulfate 7H2O5.0mg/100ml, zinc sulfate 7H2O4.4mg/100ml, magnesium sulfate 7H2O50mg/100ml。
6. the probiotics fermention Traditional Chinese medicine compound composition for the treatment of colon cancer according to claim 1, it is characterised in that described bacillus bifidus is selected from bifidobacterium bifidum, bifidobacterium breve, bifidobacterium adolescentis or bifidobacteria infantis；Described lactobacillus is selected from bacillus acidophilus, Deshi Lactobacillus, Lactobacillus plantarum, lactobacillus casei or lactobacillus rhamnosus.
7. the probiotics fermention Traditional Chinese medicine compound composition of the treatment colon cancer according to claim 1-6, it is characterized in that, for improving the mouthfeel of compositions, can be added with sweeting agent, its kind and addition in preparation method step B is sucrose 3.0g/100ml and/or sucralose 0.008g/100ml.
8. the preparation method of the probiotics fermention Traditional Chinese medicine compound composition of the treatment colon cancer described in any one of claim 1-7, it is characterised in that step is as follows:
A. taking Radix Panacis Quinquefolii powder and be broken into coarse powder, fineness is 20 order～40 orders, independent boiling twice, each 1 hour, and filtrate merges, standby；Cortex Moutan add water distillation prepare distillate standby；The medicinal residues of Radix Panacis Quinquefolii and Cortex Moutan and other Chinese medicine boiling twice, first time adds 12 times amount water soaking 1.0 hours, decoct, keep micro-after boiling to boil 2.0 hours, second time adds 10 times amount soak by water, keep micro-after boiling to boil 1.0 hours, merging filtrate, filtrate is regulated pH to 6.0, temperature is to 50 DEG C, add pectase, stir, enzymolysis 2 hours, terminate enzymolysis, 100 DEG C of enzyme denaturing 10min, merge enzymolysis solution and Radix Panacis Quinquefolii decoction liquor that to be concentrated at 60 DEG C relative density be 1.03～1.07, then concentrated solution and Cortex Moutan distilled liquid are merged to obtain traditional Chinese medicine compound extract；
B. in above-mentioned traditional Chinese medicine compound extract, add glucose, peptone, yeast extract, yeast powder and inorganic salt, add or without sweeting agent, constant volume, regulate pH value to 7.0, in 115 DEG C of autoclave sterilization 40min, obtain aseptic Chinese medicinal compound extract；
C. when above-mentioned aseptic Chinese medicinal compound extract temperature is down to 39 DEG C, inoculate one or both and above bacillus bifidus bacterium solution that pre-incubated each percent by volume is 0.5%～1.0%, after cellar culture about 2～8 hours, again inoculate one or both and above lactobacillus bacterium solution that pre-incubated each percent by volume is 0.5%～1.0%, continue cultivation 23～28 hours；Or inoculate one or both and above bacillus bifidus bacterium solution that pre-incubated each percent by volume is 0.5%～1.0% simultaneously and each percent by volume is 0.5%～1.0% one or both and above lactobacillus bacterium solution, ferment 26～40 hours；When pH value is down to below 4.1, terminates fermentation, stabilizing treatment, obtain described probiotics fermention Traditional Chinese medicine compound composition.
9. the probiotics fermention Traditional Chinese medicine compound composition of the treatment colon cancer described in any one of claim 1-7 application in the medicine of preparation treatment colon cancer.
10. application according to claim 9, it is characterised in that by described probiotics fermention Traditional Chinese medicine compound composition in accordance with known methods, make liquid preparation, capsule, tablet or granule.
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